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1.
World J Microbiol Biotechnol ; 35(9): 132, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31432260

RESUMO

This paper aims to characterize halophilic bacteria inhabiting Algerian Saline Ecosystems (Sebkha and Chott) located in arid and semi-arid ecoclimate zones (Northeastern Algeria). In addition, screening of enzymatic activities, heavy metal tolerance and antagonistic potential against phytopathogenic fungi were tested. A total of 74 bacterial isolates were screened and phylogenetically characterized using 16S rRNA gene sequencing. The results showed a heterogeneous group of microorganisms falling within two major phyla, 52 strains belonging to Firmicutes (70.2%) and 22 strains (30.8%) of γ-Proteobacteria. In terms of main genera present, the isolates were belonging to Bacillus, Halobacillus, Lentibacillus, Oceanobacillus, Paraliobacillus, Planomicrobium, Salicola, Terribacillus, Thalassobacillus, Salibacterium, Salinicoccus, Virgibacillus, Halomonas, Halovibrio, and Idiomarina. Most of the enzymes producers were related to Bacillus, Halobacillus, and Virgibacillus genera and mainly active at 10% of growing salt concentrations. Furthermore, amylase, esterase, gelatinase, and nuclease activities ranked in the first place within the common hydrolytic enzymes. Overall, the isolates showed high minimal inhibitory concentration values (MIC) for Ni2+ and Cu2+ (0.625 to 5 mM) compared to Cd2+ (0.1 to 2 mM) and Zn2+ (0.156 to 2 mM). Moreover, ten isolated strains belonging to Bacillus, Virgibacillus and Halomonas genera, displayed high activity against the pathogenic fungi (Botrytis cinerea, Fusarium oxyporum, F. verticillioides and Phytophthora capsici). This study on halophilic bacteria of unexplored saline niches provides potential sources of biocatalysts and novel bioactive metabolites as well as promising candidates of biocontrol agents and eco-friendly tools for heavy metal bioremediation.


Assuntos
Antibiose , Bactérias/isolamento & purificação , Bactérias/metabolismo , Biota , Microbiologia Ambiental , Salinidade , Argélia , Bactérias/classificação , Bactérias/genética , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Fungos/crescimento & desenvolvimento , Hidrolases/análise , Metais Pesados/metabolismo , Metais Pesados/toxicidade , Testes de Sensibilidade Microbiana , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
2.
Ann Parasitol ; 65(2): 139-144, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31376343

RESUMO

One of the most important quality characteristics associated with wastewater reuse in discharging in water bodies is the microbial quality. This study aimed to determine the efficiency of Médéa wastewater treatment plant (conventional activated sludge system) in the removal of protozoan cysts and parasitic eggs. This study was carried out during four months and samples were collected at weekly intervals from influent and effluent of the wastewater plant. In order to determine the concentration of ova, samples were analyzed according to Bailenger method. The wastewater treatment plant (WWTP) of Médéa has removed 88.9­100% of parasite eggs and more than 95% of protozoan cysts.


Assuntos
Cistos , Eucariotos , Helmintos , Esgotos , Purificação da Água , Argélia , Animais , Eucariotos/isolamento & purificação , Helmintos/isolamento & purificação , Esgotos/parasitologia , Águas Residuárias/parasitologia , Purificação da Água/métodos
3.
Water Sci Technol ; 80(1): 11-24, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31461418

RESUMO

This paper aims to assess the performance of a distributed hydrological model for simulating the transport of various heavy metals in rivers, to enhance and support environmental monitoring strategies for rivers in developing countries. In this context, we evaluated the performance of the Geophysical flow Circulation (GeoCIRC) model based on Object-Oriented Design (OOD) for the simulation of contamination from multiple heavy metals (Pb, Hg, Cr, and Zn) in Harrach River in Algeria. The results of the case study were in good agreement with the observations. Methodology for the assessment of data quality control and the improvement of monitoring procedures was proposed by using the hydrological model to simulate different scenarios. The GeoCIRC-model-based OOD allowed the prediction of the concentrations of heavy metals with minimal input data. Also, various heavy metals could be numerically treated simultaneously because the OOD increases the model's flexibility to allow the handling of many transportable materials. Therefore, the GeoCIRC model is a powerful tool for the monitoring of environmental contamination in rivers by various heavy metals.


Assuntos
Monitoramento Ambiental , Metais Pesados/análise , Poluentes Químicos da Água/análise , Argélia , China , Sedimentos Geológicos , Modelos Químicos , Medição de Risco , Rios
4.
Pan Afr Med J ; 33: 48, 2019.
Artigo em Francês | MEDLINE | ID: mdl-31448011

RESUMO

Introduction: diagnostic methods and management of iron deficiency and iron deficiency anemia in clinical practice in Algeria is poorly known. Methods: we conducted a cross-sectional survey among doctors in different specialties treating patients with iron deficiency anemia in 2016. Results: data analysis was based on 349 questionnaires which were validated (anesthesia/resuscitation: 39; obstetrics and gynaecology: 111; oncology/Hematology: 71; hepato-gastroenterology: 64; cardiology: 36; internal medicine: 28). All specialties combined, 73% (254/349) of physicians thought that at least 30% of their patients had iron deficiency anemia; 65% of physicians (226/349) thought that at least 30% of their patients had iron deficiency. Iron deficiency was investigated systematically by 57% (63/111) of physicians of the group obstetrics and gynaecology, but only by 11% (26/238) of the remaining doctors; indeed, 82% (195/238) of physicians investigated it only in patients with anemia. The assessment of iron deficiency showed that the hemoglobin (Hb) was almost always determined (89%; 310/349) while laboratory tests to explore iron metabolism were inadequate: 70% (244/349) of physicians performed serum ferritin test and only 37% (128/349) performed transferrin saturation. Patients with iron deficiency (with or without anemia) received oral iron therapy (prescribed by 92% (322/349) of physicians) and iron injections therapy depending on Hb level (prescribed by 36% (127/349) of physicians). Conclusion: this survey shows that iron deficiency is evaluated only in patients with anemia. In particular, laboratory tests to measure iron deficiency are insufficiently prescribed.


Assuntos
Anemia Ferropriva/diagnóstico , Ferro/deficiência , Médicos/estatística & dados numéricos , Padrões de Prática Médica/estatística & dados numéricos , Adulto , Argélia , Anemia/diagnóstico , Anemia/terapia , Anemia Ferropriva/terapia , Estudos Transversais , Feminino , Ferritinas/sangue , Pesquisas sobre Serviços de Saúde , Hemoglobinas/análise , Humanos , Ferro/administração & dosagem , Masculino , Pessoa de Meia-Idade , Adulto Jovem
5.
Bol. latinoam. Caribe plantas med. aromát ; 18(4): 392-410, jul. 2019. mapas, tab, graf
Artigo em Inglês | LILACS | ID: biblio-1008179

RESUMO

An ethnobotanical study was conducted with the aim to identify the medicinal plants used for the treatment of Diabetes mellitus (DM) in Sidi Bel Abbes region (Northwest Algeria). Sidi Bel Abbes, known for its diverse ecological habitats, such as mountains, steppe, lake, and fertile plains with diverse medicinal plants. The data was collected through questionnaire and interviews with inhabitants and traditional healers. The results obtained revealed that 33 plant species distributed in 20 genera belonging to 21 families for the treatment of DM were used. The most represented families were Lamiaceae, Asteraceae, Myrtaceae, Fabaceae and Lauraceae. Medicinal plants commonly used were Trigonella foenum-graecum, Olea europaea, Cinamomum cassia, Artemisia herba-alba, Lupinus albus, Juniperus communis, Rosmarinus officinalis, Prunus dulcis and Berberis vulgaris. The study revealed that, leaves, followed by seeds and stem bark were mostly used parts. Also, decoction and infusion were the most frequently used method of preparation. This study confirms that most people with Diabetes mellitus in the study areas rely on traditional medicine for their primary health care needs.


Se realizó un estudio etnobotánico con el objetivo de identificar las plantas medicinales utilizadas para el tratamiento de la Diabetes mellitus (DM) en la región de Sidi Bel Abbes (noroeste de Algeria). Sidi Bel Abbes, conocida por sus diversos hábitats ecológicos, como montañas, estepas, lagos y fértiles llanuras con plantas medicinales. Los datos se recopilaron mediante cuestionarios y entrevistas con habitantes y curanderos tradicionales. Los resultados obtenidos revelaron que 33 especies vegetales distribuidas en 20 géneros pertenecientes à 21 familias fueron utilizadas para el tratamiento de DM. Las familias más representadas fueron Lamiaceae, Asteraceae, Myrtaceae, Fabaceae y Lauraceae. Las plantas medicinales comúnmente utilizadas fueron Trigonella foenum-graecum, Olea europaea, Cinamomum cassia, Artemisia herba-alba, Lupinus albus, Juniperus communis, Rosmarinus officinalis, Prunus amygdalus y Berberis vulgaris. El estudio reveló que las hojas, seguidas de las semillas y la corteza de tallo, eran en su mayoría partes usadas. Sin embargo, la infusión y la decocción son el método de preparación más utilizado. El estudio reveló que las hojas, seguidas de las semillas y las partes del área, eran en su mayoría partes utilizadas. Tambien, la decocción y la infusión fueron los métodos de preparación más utilizado. Este estudio confirma que la mayoría de las personas con Diabetes mellitus en las áreas d'estudio dependen de la medicina tradicional para sus necesidades d'atenciónn primaria de la salud.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Plantas Medicinais , Folhas de Planta , Etnobotânica , Preparações de Plantas/uso terapêutico , Diabetes Mellitus/tratamento farmacológico , Sementes , Extratos Vegetais/uso terapêutico , Inquéritos e Questionários , Argélia , Medicina Tradicional
6.
Pan Afr Med J ; 32: 197, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31312309

RESUMO

Introduction: Just recently, it has been established that the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism is linked to the pathogenesis and to the evolution of human cancers. Therefore, the present study was concerned with the investigation of an eventual association between glioma and I/D polymorphism of the ACE gene. Methods: The expression of ACE gene was detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis in 36 Algerian patients with glioma and 195 healthy controls. Results: In glioma cases, allelic frequencies and genotypes distribution of the ACE I/D polymorphism were different from controls cases. ACE DD genotype were highly presented in glioma cases (63.9%) than controls (33.8%) and conferred 3.64-fold risk for predisposition in glioma cases (vs ID genotype, p<0.001). Recessive model (ACE II + ID genotypes vs DD) was associated with a 72% reduced risk of glioma (OR = 0.28, 95% CI: 0.13-0.60, p <0.001). Per copy D allele frequency was found higher in glioma cases (79.2%) than in controls (63.3 %), OR = 2.20, 95% CI: 1.20 - 4.03, p = 0.009. Conclusion: The obtained data showed that the presence of the D allele might be a risk factor for the development of glioma. Further studies considering different ethnic groups with large samples are required to confirm this finding.


Assuntos
Neoplasias Encefálicas/genética , Predisposição Genética para Doença , Glioma/genética , Peptidil Dipeptidase A/genética , Adulto , Argélia , Estudos de Casos e Controles , Feminino , Deleção de Genes , Frequência do Gene , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Mutagênese Insercional , Reação em Cadeia da Polimerase , Polimorfismo Genético , Polimorfismo de Fragmento de Restrição , Fatores de Risco
7.
BMC Complement Altern Med ; 19(1): 146, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31227024

RESUMO

BACKGROUND: There is increasing interest in the pharmaceutical and food industries to substitute synthetic chemicals with naturally occurring compounds possessing bioactive properties. Plants are valuable sources of bioactive compounds. The present study investigates the chemical composition and antioxidant, antimicrobial, and anticancer activities of ethanolic extracts (EEs) and essential oils (EOs) from two species in the Lamiaceae family, Ocimum basilicum L. and Thymus algeriensis Boiss. & Reut., cultivated in the Algerian Saharan Atlas. METHODS: The total flavonoid contents of the plants' ethanolic extracts were determined by the aluminium chloride method, while the total phenols were determined using the Folin-Ciocalteu method. Essential oils were obtained by hydrodistillation of the aerial parts of the plants and were analysed by GC-MS. The free radical-scavenging ability and antioxidant potential of the plants' EEs and EOs were probed using the 2, 2-diphenyl-picrylhydrazyl radical-scavenging, ABTS radical-scavenging, ferric-reducing power and phosphomolybdenum assays. The antimicrobial activities were evaluated against several pathogens characteristic of gram-negative bacteria (three species), gram-positive bacteria (three species) and fungi (two species). The microdilution method was used to estimate the minimum inhibitory concentrations (MICs). The oils' anticancer potential against several cancer types was also studied using the MTT assay and reported as the toxic doses that resulted in a 50% reduction in cancer cell growth (LD50). RESULTS: Phenolic compounds in the EEs from both plants were analysed by HPLC and demonstrated a rich flavonoid content. Chemical analysis of the essential oil from Ocimum basilicum revealed 26 unique compounds, with linalool (52.1%) and linalyl acetate (19.1%) as the major compounds. A total of 29 compounds were identified in the essential oil from Thymus algeriensis, with α-terpinyl acetate (47.4%), neryl acetate (9.6%), and α-pinene (6.8%) as the major compounds. The ethanolic extracts and essential oils from both plants exhibited moderate antioxidant activities and moderate to weak antimicrobial activities. Furthermore, anticancer activities against the examined human cancer cell lines were associated with only the EOs from both plants, with LD50 values ranging between 300 and 1000 µg/mL. CONCLUSION: The results suggest that the bioactive compounds found in the ethanolic extracts and essential oils from Ocimum basilicum and Thymus algeriensis, with diverse antioxidant, antimicrobial and anticancer activities, may have beneficial applications in nutraceutical and pharmaceutical technologies.


Assuntos
Ocimum basilicum/química , Óleos Voláteis/química , Óleos Vegetais/química , Thymus (Planta)/química , Argélia , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Linhagem Celular Tumoral , Sobrevivência Celular , Cromatografia Líquida de Alta Pressão , Fungos/efeitos dos fármacos , Fungos/crescimento & desenvolvimento , Humanos , Fenol/química , Fenol/farmacologia , Óleos Vegetais/farmacologia
8.
Water Sci Technol ; 79(7): 1357-1366, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31123235

RESUMO

This work concerns the elimination of the organic pollutant; Bemacid Red (BR), a rather persistent dye present in wastewater from the textile industry in western Algeria, by adsorption on carbon from an agricultural waste in the optimal conditions of the adsorption process. An active carbon was synthesized by treating an agro-alimentary waste, the date stones that are very abundant in Algeria, physically and chemically. Sample after activation (SAA) with phosphoric acid was highly efficient for the removal of BR. The characterization of this porous material has shown a specific surface area that exceeds 900 m2/g with the presence of mesopores. The iodine value also indicates that the activated carbon obtained has a large micro porosity. The reduction of the infrared spectroscopy (FTIR) bands reveals that the waste has been synthesized and activated in good conditions. Parameters influencing the adsorption process have been studied and optimized, such as contact time, adsorbent mass, solution pH, initial dye concentration and temperature. The results show that for a contact time of 60 min, a mass of 0.5 g and at room temperature, the adsorption rate of the BR by the SAA is at its maximum. Pseudo-first-order, pseudo-second-order and intraparticle diffusion models were studied to analyse adsorption kinetics. The result shows the adsorption kinetic is best with the pseudo-second-order model. In this study, Langmuir, Freundlich and Temkin isotherms were investigated for adsorption of BR onto SAA. The Freundlich and Temkin isotherms have the highest correlations coefficients. The suggested adsorption process involves multilayer adsorption with the creation of chemical bonds. The mechanism of adsorption of BR by SAA is spontaneous and exothermic, and the Gibbs free energy values confirm that the elimination of the textile dye follows a physisorption.


Assuntos
Carvão Vegetal/química , Corantes/química , Poluentes Químicos da Água/química , Purificação da Água/métodos , Adsorção , Argélia , Carbono , Concentração de Íons de Hidrogênio , Cinética , Termodinâmica , Poluentes Químicos da Água/análise
9.
Ticks Tick Borne Dis ; 10(4): 924-928, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31080140

RESUMO

Livestock and their ectoparasites are involved in the epidemiology of several zoonotic diseases. Studies regarding the molecular detection of infectious agents in ticks from Northwestern Algeria are scarce. Thus, the presence of spotted fever group Rickettsia spp., Anaplasmataceae microorganisms and Coxiella burnetii was investigated in ticks collected from ruminants in Sidi Bel Abbes and Saida provinces. Rickettsia aeschlimannii was detected in one Hyalomma excavatum pool and one H. marginatum pool. Moreover, 'Candidatus Rickettsia barbariae' was found in one H. excavatum and six Rhipicephalus bursa pools. Lastly, Coxiella burnetii was amplified in two H. excavatum and two R. bursa pools. No Anaplasmataceae bacterium was detected. This study demonstrates the presence of the tick-associated microorganism 'Candidatus R. barbariae' in the North of Africa, and corroborates the presence of the zoonotic pathogens R. aeschlimannii and C. burnetii in Algeria.


Assuntos
Coxiella burnetii/genética , Ixodidae/microbiologia , Gado/parasitologia , Rickettsia/genética , Infestações por Carrapato/veterinária , Argélia , Animais , Proteínas da Membrana Bacteriana Externa/genética , Coxiella burnetii/isolamento & purificação , DNA Bacteriano/genética , Feminino , Ixodidae/genética , Gado/microbiologia , Filogenia , Reação em Cadeia da Polimerase , Rickettsia/isolamento & purificação , Ruminantes/microbiologia , Ruminantes/parasitologia
10.
Work ; 62(4): 657-665, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31104049

RESUMO

BACKGROUND: Despite the call for the application of ergonomics in developing countries, the African share of ergonomic studies is modest. Date palm farming is considered one of the most important economic resources in hot and dry areas. In African countries, including Algeria, there exist millions of date palms. Date palm work can be precarious and associated with higher rates of work related musculoskeletal disorders. OBJECTIVE: This paper aims to:1.Reveal how the workers climb the trunk of the date palm to get to the crown.2.Detect the amount of work related musculoskeletal disorders (WRMSDs) in date palm trees work, and how to combat it. METHOD: The descriptive method was used. RESULTS: Objective 1, Majority of date palm workers are still using the traditional methods, i.e. free climbing, and belt aided climbing. However, a minority of workers have started using modern methods i.e. ladders, climbing devices, and modern technology (e.g. hydraulic lifts). Objective 2, the workers complained about WRMSDs presence in the shoulders, hands, wrists, lower back, hips knees, and feet. Efforts to combat these WRMSDs are personal, educational and scientific efforts. CONCLUSION: Regarding climbing, the traditional methods are still dominant, and the use of technology is very limited. As regards WRMSDs, date palm workers complain about their presence in many parts of the body.


Assuntos
Agricultura/métodos , Ergonomia/normas , Agricultura/estatística & dados numéricos , Argélia/epidemiologia , Ergonomia/métodos , Fazendeiros/estatística & dados numéricos , Humanos , Traumatismos Ocupacionais/epidemiologia , Traumatismos Ocupacionais/etiologia , Fatores de Risco , Árvores
11.
Chem Biodivers ; 16(7): e1900094, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31099458

RESUMO

This review updates the information upon the chemical composition of propolis from all Mediterranean countries as well as their biological properties and applications. The non-volatile fraction of propolis was characterized by the presence of phenolic acids and their esters and flavonoids. Nevertheless, in some countries, diterpenes were also present: Sicily (Italy), Croatia, Malta, Creta (Greece), Turkey, Cyprus, Egypt, Libya, Algeria and Morocco. The volatile fraction of propolis was characterized by the presence of benzoic acid and its esters, mono- and sesquiterpenes, being the oxygenated sesquiterpene ß-eudesmol characteristic of poplar propolis, whereas the hydrocarbon monoterpene α-pinene has been related with the presence of conifers. Regardless the chemical composition, there are common biological properties attributed to propolis. Owing to these attributes, propolis has been target of study for applications in diverse areas, such as food, medicine and livestock.


Assuntos
Própole/farmacologia , Argélia , Chipre , Egito , Grécia , Itália , Líbia , Marrocos , Própole/química , Própole/isolamento & purificação , Turquia
12.
Vet Ital ; 55(1): 35-46, 2019 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-30951180

RESUMO

The objective of the study was to determine the virulence and antimicrobial resistance traits of 100 fecal E. coli strains isolated from clinically healthy chickens in Algeria. Most of isolates belonged to phylogroups A (45%) and B1 (37%) and showed a great diversity in DNA profiles. The genes fimH, tsh, entB, iutA, irp2, fyuA, iroN, sitA, etsA, etsB, eitA, iss, traT, ompT, hlyF, vat, ibeA, cvaA, cvaB5', cvaB3', cvaC, cma and cbi were detected. Combinations of virulence genes defined 67 virulence profiles. High resistance rates (62­97%) were noted for amoxicillin, amoxicillin­clavulanic acid, cefazolin, fluoroquinolones, tetracycline, trimethoprim, sulfonamides and sulfamethoxazole/ trimethoprim, and 93% of strains were multidrug­resistant. Combinations of resistance phenotypes defined 59 resistance patterns. The genes blaTEM, blaSHV, blaCTX­M­1, tetA, tetB, qnrB, qnrS1, sul1, sul2, sul3, dfrA1, dfrA7, dfrA12 and dfrA14 were identified and class 1 integrons were detected in 49% of isolates. A rate of 37% of strains was resistant to mercury, with the presence of merA gene. The study reports the presence in the avian strains isolated from fecal swabs of virulence genes of plasmid origin characteristic of ExPEC strains associated with high resistance to first­line antibiotics and class 1 integrons, this augurs a risk for human and animal health.


Assuntos
Galinhas , Farmacorresistência Bacteriana/genética , Infecções por Escherichia coli/veterinária , Escherichia coli/isolamento & purificação , Perfil Genético , Doenças das Aves Domésticas/epidemiologia , Argélia/epidemiologia , Animais , Anti-Infecciosos/farmacologia , Escherichia coli/classificação , Escherichia coli/genética , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Fezes/microbiologia , Filogenia , Doenças das Aves Domésticas/microbiologia , Virulência/genética
13.
Arch Environ Contam Toxicol ; 77(1): 127-143, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31020374

RESUMO

In this study, 28 surface water samples were collected from eight different sites throughout the Chott Merouane. Samples were detected by atomic absorption spectrometry for Cd, Co, Cr, Cu, Fe, Ni, Mn, Pb, and Zn. The dissolved metal concentrations (mg/L) ranged from 0.05 to 0.90 mg/L for Cd, 0.13-6.45 mg/L for Co, bDL-2.05 mg/L for Cr, 0.03-0.27 mg/L for Cu, 0.34-7.41 mg/L for Fe, 01.6-4.54 mg/L for Ni, 0.15-1.19 mg/L for Mn, 0.23-5.88 mg/L for Pb, and 0.01-0.28 mg/L for Zn. Compared with U.S. EPA standards and other freshwaters worldwide, the most mean concentration of metals in surface water of this salt lake are higher than the guideline levels of aquatic life. This was further corroborated by results from the water quality indices that Chott Merouane is seriously polluted by metals. The values of the metal pollution index indicated that metal pollution level was Cd > Pb > Ni > Fe > Cr > Cu > Mn > Zn, and those metals belong to moderate or high pollution level. The Nemerow pollution index further indicated that Chott Merouane was suffering from serious metal contamination. Based on geostatistics analyses, generally distributions of these metal contents decreased in the order of the North Chott Merouane ≈ the Northwest Chott Merouane > the Eastern part of Chott Merouane > the South Chott Merouane. The quality of water has drastically deteriorated due to the mixed source of anthropogenic inputs. Therefore, necessary conservation and management measures should be taken to improve the water quality of this Ramsar wetland.


Assuntos
Metais/análise , Poluentes Químicos da Água/análise , Argélia , Monitoramento Ambiental/métodos , Metais/toxicidade , Medição de Risco , Poluentes Químicos da Água/toxicidade , Qualidade da Água
14.
Can J Microbiol ; 65(6): 405-420, 2019 Jun.
Artigo em Francês | MEDLINE | ID: mdl-30935210

RESUMO

In the dairy industry, bacterial contaminants persist on equipment surfaces due to spore and biofilm formation. These are involved in cross-contamination problems that affect the quality of processed products and limit their life. The pasteurization process, in which milk is submitted to moderate heat treatment, is inefficient against bacterial spores. The most prevalent sporulated bacteria belong to Bacillus and related genera. The situation is more complicated in countries where pasteurized milk is derived from imported milk powder originally contaminated by bacterial spores. Studies have shown biofilm formation on dairy equipment by mesophilic strains from the group Bacillus cereus and thermophilic strains from the genus Geobacillus. These biofilms are resistant to cleaning procedures and are sources of chronic contamination of pasteurized milk. This review analyzes the dairy situation in Algeria exposed to sporulated flora and derived biofilm problems, with the aim of proposing efficient solutions in the light of current knowledge. [Journal translation].


Assuntos
Biofilmes , Indústria de Laticínios , Leite/microbiologia , Argélia , Animais , Bacillus/isolamento & purificação , Esporos Bacterianos
15.
Work ; 62(3): 435-442, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30909260

RESUMO

BACKGROUND: There is a consensus in the evidence literature on the importance of Quality of Work Life (QWL) as it is a prerequisite to increase employees' productivity and wellbeing [2], [1], [11]. While studies of the effect of demographic characteristics (gender, age, work experience and socio-professional categories) on QWL have conflicting results [1], [14], [15], [16], particularly in the services (tertiary) sector of activity, which is economically considered as the most important sector, as it encompasses a large spectrum of activities. OBJECTIVE: This paper aims to measure the level of QWL and to study the differences in demographic characteristics (gender, age, work experience and socio-professional category) among managerial staff. METHODS: Managerial staff members in the Algerian tertiary sector (N = 252) participated in the study. Data were collected using a questionnaire. RESULTS: The analysis of the results suggested that the level of QWL was average (x¯= 253.61, SD = 46.775), on an ordinal scale ranging from a score value of 75 (min.) to 375 (max.) as shown in Table 2, and, there were no statistically significant differences in QWL between demographic characteristics categories: (1) gender, (2) age, (3) work experience and (4) socio-professional categories. The results are discussed in the light of previous researches. CONCLUSIONS: The study concluded that the QWL needs more attention from management levels in the public sector in Algeria.


Assuntos
Administração de Consultório/normas , Qualidade de Vida/psicologia , Adulto , Argélia , Estudos Transversais , Demografia/métodos , Emprego/psicologia , Emprego/normas , Feminino , Humanos , Indústrias/métodos , Satisfação no Emprego , Masculino , Pessoa de Meia-Idade , Inquéritos e Questionários
16.
Parasitol Res ; 118(5): 1417-1428, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30915549

RESUMO

The monogenean Microcotyle erythrini is atypical because it has been recorded from several fish host species in the Mediterranean Sea and Atlantic Ocean, in contrast to many species which are considered strictly specific. This could indicate a true lack of specificity or that several cryptic species are involved. This paper is a partial attempt to solve this problem. Specimens of a monogenean resembling M. erythrini were collected from bogues, Boops boops, caught off Algeria. A comparison with published descriptions and with museum specimens of M. erythrini did not yield any clear morphological difference. However, sequences of cytochrome c oxidase subunit I (COI) differed by 16.3% from that of M. erythrini (from GenBank, material collected from the type-host Pagellus erythrinus), indicating that the species was different. The species from B. boops is therefore described here as Microcotyle isyebi n. sp. and differential diagnoses with Microcotyle species from the Mediterranean and from sparids are provided. These results suggest that a molecular re-evaluation of other M. erythrini-like specimens from various fish hosts could reveal the existence of additional parasite biodiversity.


Assuntos
Complexo IV da Cadeia de Transporte de Elétrons/genética , Doenças dos Peixes/parasitologia , Perciformes/parasitologia , Trematódeos/classificação , Argélia , Animais , Oceano Atlântico , Peixes/parasitologia , Mar Mediterrâneo , Trematódeos/anatomia & histologia , Trematódeos/isolamento & purificação
17.
Bull Environ Contam Toxicol ; 102(4): 555-559, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30859248

RESUMO

The aim of this work is to assess biomarker responses to stresses caused by pollution of the marine environment in the Gulf of Annaba, using the bivalve mollusc Donax trunculus and Mytilus galloprovincialis. Males and females were collected during the summer from site 1 considered to be far from pollution sources (SM1 and SD1), site 2 (SM2 and SD2) exposed to a mixture of contaminants, and site 3 (SM3 and SD3) receiving the untreated sewage. The whole body was used for the determination of malonaldehyde (MDA), reduced glutathione (GSH) and DNA damage. The MDA concentration from site 2 of both species has indicated significant augmentation. The two bivalves from S2 and S3 have recorded significant decrease of GSH level, with the highest number of Comet scores. A significant site and sex effect was measured for the level of GSH and MDA, accompanied with a significant site effect for DNA damage. The genotoxicity seems correlated with the stress biomarkers and dependent on the effluent types.


Assuntos
Bivalves/efeitos dos fármacos , Monitoramento Ambiental , Poluentes Químicos da Água/toxicidade , Argélia , Animais , Biomarcadores , Dano ao DNA , Feminino , Glutationa , Masculino , Malondialdeído , Especificidade da Espécie , Poluentes Químicos da Água/análise
18.
Parasitol Res ; 118(4): 1179-1192, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30847611

RESUMO

The liver fluke Fasciola hepatica is the main cause of fasciolosis in North Africa leading to significant economic losses and public health problems. In this study, the ribosomal internal transcribed spacer (ITS), cytochrome c oxidase I (COI), the mitochondrial region spanning the COI-trnT-rrnL, and the NADH dehydrogenase subunit I (NADI) markers were used to characterize Fasciola flukes from Algeria. Fasciola appeared widespread from the east to the west of Algeria. Among 1701 sampled cattle from 8 Algerian provinces, 5% were infected. Using morphological and morphometric analysis, one morphotype of Fasciola was observed. Nuclear ITS marker indicated that all collected flukes belong to F. hepatica. Multiple alignments of ITS dataset revealed two haplotypes, one described here for the first time. Analysis of molecular variance (AMOVA) of mitochondrial markers revealed weak population structure in Algeria. Mismatch distributions, neutrality tests, and median-joining network analysis all were compatible with a recent expansion of Algerian F. hepatica population. Fasciolosis appeared common in Algerian cattle, it seems that the absence of control strategy coupled to the favorable Mediterranean climate may lead to a reconstruction and dispersion of its populations. This study provides important results concerning the genetic characterization and variability of F. hepatica in Algeria as well as the significant role of cattle importation in shaping its dispersal route worldwide.


Assuntos
Doenças dos Bovinos/parasitologia , DNA de Helmintos/genética , Fasciola hepatica/genética , Fasciolíase/veterinária , Variação Genética/genética , Argélia , Animais , Bovinos , DNA Espaçador Ribossômico/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Fasciola hepatica/isolamento & purificação , Haplótipos/genética , NADH Desidrogenase/genética , Filogenia , Reação em Cadeia da Polimerase
19.
Onderstepoort J Vet Res ; 86(1): e1-e3, 2019 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-30843409

RESUMO

Clinical indicators such as diarrhoea (DISCO) or anaemia (FAMACHA©) are used as a measure for targeted selective treatments against gastrointestinal nematodes (GIN). Enteric cestodes such as Moniezia may interfere directly with DISCO or indirectly with the FAMACHA© score. We investigated 821 Ouled Djellal rams naturally infected in a steppe environment (GIN alone, cestodes alone, GIN and cestodes) or not. The rams were treated with ivermectin 2 months before being slaughtered to reduce the impact of nematodes on the clinical scores; however, persistent or newly acquired GINs were not related to both scores. Of the non-infected rams (n = 296), 26% identified as needing treatment against GIN using the FAMACHA score, and 34.5% using DISCO would have been thus selected. This implies that the clinical indicators used for the targeted selective treatment of gastrointestinal nematodes are not fully reliable when a low infection is recorded and may well be influenced by confounding factors. As expected, only DISCO was affected by cestode infection, and we suggest that the presence of Moniezia should also be taken into consideration.


Assuntos
Anti-Helmínticos/uso terapêutico , Infecções por Cestoides/veterinária , Ivermectina/uso terapêutico , Infecções por Nematoides/veterinária , Doenças dos Ovinos/tratamento farmacológico , Doenças dos Ovinos/epidemiologia , Argélia/epidemiologia , Animais , Cestoides/efeitos dos fármacos , Infecções por Cestoides/tratamento farmacológico , Infecções por Cestoides/epidemiologia , Infecções por Cestoides/parasitologia , Enteropatias Parasitárias/tratamento farmacológico , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Enteropatias Parasitárias/veterinária , Masculino , Nematoides/efeitos dos fármacos , Infecções por Nematoides/tratamento farmacológico , Infecções por Nematoides/epidemiologia , Infecções por Nematoides/parasitologia , Prevalência , Ovinos , Doenças dos Ovinos/parasitologia
20.
Microbiol Res ; 221: 60-69, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30825942

RESUMO

Bacteria isolated from different environments can be exploited for biocontrol purposes by the identification of the molecules involved in the antifungal activity. The present study was aimed at investigating antifungal protein compounds purified from a previously identified plant growth promoting bacterium, Pseudomonas protegens N isolated from agricultural land in northern Algeria. Therefore, a novel protein was purified by chromatographic and ultrafiltration steps and its antifungal activity together with growth-inhibition mechanism was evaluated against different fungi by plate-based assays. In addition, stereomicroscopy and transmission electron microscopy (TEM) was performed to explore the inhibition activity of the compound on spore germination processes. The protein, showing a molecular mass of about 100 kDa under native conditions, was revealed to be in the surface-membrane fraction and displayed an efficient activity against a variety of phytopathogenic fungi, being Alternaria the best target towards which it exhibited a marked fungicidal action and inhibition of spore germination. Moreover, the compound was able to significantly decrease fungal infection on tomato fruits producing also morphological aberrations on conidia. The obtained results suggested that the isolated compound could represent a promising agent for eco-friendly management of plant pathogens in agriculture.


Assuntos
Alternaria/crescimento & desenvolvimento , Antifúngicos/farmacologia , Agentes de Controle Biológico/farmacologia , Pseudomonas/metabolismo , Esporos Fúngicos/crescimento & desenvolvimento , Argélia , Alternaria/efeitos dos fármacos , Lycopersicon esculentum/microbiologia , Testes de Sensibilidade Microbiana , Desenvolvimento Vegetal/fisiologia , Reguladores de Crescimento de Planta/metabolismo , Plantas/microbiologia , Pseudomonas/isolamento & purificação , Rizosfera , Microbiologia do Solo , Esporos Fúngicos/efeitos dos fármacos
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