Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.993
Filtrar
1.
Microbiol Res ; 228: 126327, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31499402

RESUMO

The aim of this work was to investigate the early detection of anthracnose and soft rot diseases in cold stored strawberry fruit by evaluating the CO2 and volatile organic compounds (VOCs) released by the fungi Colletotrichum fragariae and Rhizopus stolonifer. Strawberries were stored at 5, 10 and 21 °C (control group) and the VOCs and CO2 production of inoculated and non-inoculated strawberries were followed by gas chromatography. To evaluate and estimate the growth of both fungi, the CO2 data were fitted to the Gompertz model. Data of the VOCs released at the end of the fungal growth were analyzed using principal components analysis (PCA) to discriminate between infected and non-infected strawberries. The results showed that fungal growth was affected by temperature and C. fragariae had a maximum growth after 14.6 h at 5 °C and R. stolonifer at 21 °C after 45.2 h. On the other hand, through VOCs released by C. fragariae and R. stolonifer and PCA, four groups were obtained: a) strawberry infected with C. fragariae, stored at 10 °C, b) strawberry infected with R. stolonifer, stored at 21 °C, c) control group kept at 10 °C and, d) strawberry infected with C. fragariae and control group (5 and 21 °C), and strawberry infected with R. stolonifer at 5 and 10 °C. In conclusion, CO2 and VOCs released by C. fragariae and R. stolonifer on strawberries could infer the presence of anthracnose and soft rot during storage of the fruit at low temperature.


Assuntos
Dióxido de Carbono/metabolismo , Temperatura Baixa , Colletotrichum/metabolismo , Fragaria/microbiologia , Frutas/microbiologia , Rhizopus/metabolismo , Compostos Orgânicos Voláteis/metabolismo , Dióxido de Carbono/análise , Armazenamento de Alimentos , Análise Multivariada , Doenças das Plantas/microbiologia , Rhizopus/crescimento & desenvolvimento , Compostos Orgânicos Voláteis/antagonistas & inibidores
2.
Food Chem ; 300: 125231, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31374430

RESUMO

This research was to compare mortadella elaborated with synthetic antioxidant and microcrystals of curcumin in relation to its physicochemical and sensorial characteristics for a period of 90 days. It was detect no differences between the three evaluated treatments in relation to the pH, color, and texture profile features. The mortadella with curcumin microcrystals showed significantly lower TBARS values at the end of the storage when compared to the other treatments. In the sensory analysis, the addition of curcumin decreased the acceptance of color's sample and the purchase intention, but no significant difference was observed among the other attributes. The color of the sample containing curcumin also became worse than its day-of-production standard during storage. The results obtained suggest the potential of curcumin in replacing synthetic antioxidants in cooked meat sausage, since it practically does not modify its physicochemical characteristics, besides preventing the oxidation of the food.


Assuntos
Antioxidantes/química , Curcumina/química , Produtos da Carne , Ácido Ascórbico/química , Cor , Comportamento do Consumidor , Culinária , Armazenamento de Alimentos , Humanos , Concentração de Íons de Hidrogênio , Produtos da Carne/análise
3.
Food Chem ; 298: 125093, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260960

RESUMO

The purpose of this study was to investigate the impact of ozonation process on the level of oxidative stress markers in raspberries stored at room temperature. Raspberry fruit was ozonated with an ozone concentration of 8-10 ppm for 30 min, every 12 h, for 72 h of storage at room temperature. Research showed that ozonated raspberries were characterized by higher activity of superoxide dismutase, ascorbate peroxidase and phenylalanine ammonia-lyase. In turn, the ability to generate superoxide anion radical and hydrogen peroxide by ozone-treated fruit was significantly lower than in the control sample due to higher activities of ROS detoxification systems.


Assuntos
Armazenamento de Alimentos/métodos , Frutas/metabolismo , Estresse Oxidativo , Ozônio/química , Rubus/metabolismo , Antioxidantes/química , Antioxidantes/metabolismo , Ascorbato Peroxidases/metabolismo , Biomarcadores/química , Biomarcadores/metabolismo , Frutas/química , Peróxido de Hidrogênio/química , Peróxido de Hidrogênio/metabolismo , Fenilalanina Amônia-Liase/metabolismo , Espécies Reativas de Oxigênio/química , Espécies Reativas de Oxigênio/metabolismo , Rubus/química , Superóxido Dismutase/metabolismo , Superóxidos/química , Superóxidos/metabolismo , Temperatura Ambiente
4.
Food Chem ; 298: 125083, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31261001

RESUMO

The effects of onion or caraway on changes in the content of biogenic amines were examined in sauerkraut during a fermentation process at 18 °C or 31 °C for 14 days and subsequent storage at 4 °C for 12 weeks. The amines were analysed by high-performance liquid chromatography with pre-column benzoylation. Total biogenic-amine concentration at the end of the fermentation was lower at 31 °C than at 18 °C. However, at this lower temperature, the presence of caraway or onion more significantly (than at 31 °C) reduced the total biogenic-amine content as compared to the control sample without an additive. After 12 weeks of refrigerated storage, concentrations of phenethylamine, tryptamine, and tyramine in the sauerkraut fermented with caraway (and concentrations of putrescine and tryptamine in the sauerkraut fermented with onion) at 31 °C increased as compared to the samples on the last day of fermentation, but did not pose a risk for consumer health.


Assuntos
Aminas Biogênicas/metabolismo , Carum , Alimentos Fermentados , Armazenamento de Alimentos/métodos , Cebolas , Aminas Biogênicas/análise , Brassica , Cromatografia Líquida de Alta Pressão , Temperatura Baixa , Fermentação , Alimentos Fermentados/análise , Humanos , Concentração de Íons de Hidrogênio , Putrescina/análise , Putrescina/metabolismo , Paladar , Tiramina/análise , Tiramina/metabolismo
5.
Food Chem ; 298: 125098, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31276942

RESUMO

This work aimed to optimize an aqueous extract rich in phenolic compounds and potential functional properties made of Ilex paraguariensis, Melissa officinalis, and Cymbopogon citratus. The lyophilized extract was used for the development of an ice cream. Total phenolics, FRAP, DPPH, Folin-Ciocalteu's reducing capacity, and total reducing capacity of different combinations of herbal extracts were tested and modeled using response surface methodology. Simultaneous optimisation was employed to maximize the bioactive compounds in the extract and the lyophilized optimum combination was added to ice cream. The lyophilized extract contained quercetin-3-rutinoside, hesperidin, isoquercetin, caffeic acid, and 5,7-dihydroxyflavone. The optimised extract, which showed antihypertensive, antidiabetic, and antioxidant activity using in vitro protocols, increased total phenolics and antioxidant activity in comparison to the control ice cream. The ice cream presented a sensory acceptance index of 83%. After 72 days of storage (-18 °C), total phenolics and antioxidant activity significantly decreased.


Assuntos
Indústria de Processamento de Alimentos/métodos , Sorvetes , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Adolescente , Adulto , Anti-Infecciosos/farmacologia , Anti-Hipertensivos/química , Anti-Hipertensivos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Linhagem Celular , Cymbopogon/química , Feminino , Flavonoides/análise , Flavonoides/química , Armazenamento de Alimentos , Humanos , Sorvetes/análise , Ilex paraguariensis/química , Masculino , Melissa/química , Pessoa de Meia-Idade , Oligossacarídeos/química , Fenóis/química , Paladar
6.
Food Chem ; 299: 125116, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31295637

RESUMO

The effects of exogenous melatonin treatment on the enzymatic browning and nutritional quality of fresh-cut pear fruit were investigated. Fresh-cut fruit soaked with 0, 0.05, 0.1 and 0.5 mM melatonin were stored at 4 °C. Our results showed that 0.1 mM melatonin treatment was optimal for reducing the surface browning and maintaining the titratable acidity of the fresh-cut fruit, which significantly decreased MDA and H2O2 contents and the growth of microorganism, enhanced total phenolic content and antioxidant capacity, and delayed the reduction of ascorbic acid. Furthermore, melatonin treatment at 0.1 mM decreased the expression of genes involving in enzymatic browning pathway including POD, PPO1, PPO5 and LOX1, and reduced PPO activity. Moreover, this treatment increased the expression of PAL and CHS, and enhanced PAL and CHS activities. These results showed that melatonin treatment might be a promising strategy to alleviate browning and improve the nutritional quality of fresh-cut pear fruit.


Assuntos
Frutas/efeitos dos fármacos , Melatonina/farmacologia , Valor Nutritivo , Pyrus/efeitos dos fármacos , Antioxidantes/análise , Antioxidantes/metabolismo , Ácido Ascórbico/análise , Ácido Ascórbico/metabolismo , Armazenamento de Alimentos , Frutas/química , Frutas/metabolismo , Frutas/microbiologia , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Peróxido de Hidrogênio/análise , Peróxido de Hidrogênio/metabolismo , Malondialdeído/análise , Malondialdeído/metabolismo , Fenóis/análise , Pyrus/química , Pyrus/genética , Pyrus/metabolismo
7.
Food Chem ; 298: 125017, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260967

RESUMO

The aim of the study was to evaluate an elevated (3.0 °C) and low (1.0 °C) storage temperature combined with dynamic controlled atmosphere monitored by respiratory quotient (DCA-RQ) and chlorophyll fluorescence (DCA-CF) on anaerobic metabolism, physiological storage disorders and overall quality of 'Nicoter' ('Kanzi®') apples after 5.5 and 8.0 months of storage plus 7d shelf-life. Fruit stored under DCA-RQ 2.0 accumulated the highest amounts of anaerobic metabolites (acetaldehyde, ethanol and ethyl acetate), regardless of storage temperature and timing of storage outturn evaluation, but it did not result in higher electrolyte leakage. Flesh breakdown, core breakdown and cavity formation were reduced at 3 °C. Storage at 3 °C combined with DCA maintained higher flesh firmness after 8.0 months storage plus 7d shelf-life. 'Nicoter' apples can be stored at 3 °C using a DCA system, based either on CF or on RQ, to save electrical energy.


Assuntos
Armazenamento de Alimentos/métodos , Frutas/metabolismo , Malus/metabolismo , Acetaldeído/metabolismo , Acetatos/metabolismo , Anaerobiose , Atmosfera , Clorofila/metabolismo , Etanol/metabolismo , Fluorescência , Temperatura Ambiente
8.
Food Chem ; 298: 125019, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260984

RESUMO

This study aims to investigate the postharvest physiology and texture of garlic cloves packaged in polyethylene terephthalate (PET), polyethylene (PE), aluminized kraft paper (AKP), single kraft paper (SKP), and mesh bag. Germination rate, electrical conductivity, respiration intensity, water content, and texture were determined during 180 d storage at -2 °C. Results showed that the germination of garlic cloves packaged in PET, PE, and AKP was effectively inhibited during storage. PE effectively reduced the degree of damage to the cell membranes of the garlic cloves. PE and SKP significantly inhibited respiratory intensity during storage. Garlic cloves water content did not change significantly in 90 d storage which packaged in PE and SKP. PE exhibited better effect on the texture and freshness of garlic cloves than the other materials. In conclusion, PE is the best packaging material for maintaining the quality attributes and extending the shelf lives of garlic cloves.


Assuntos
Embalagem de Alimentos , Alho/fisiologia , Polietileno , Condutividade Elétrica , Armazenamento de Alimentos , Germinação , Polietilenotereftalatos , Refrigeração , Água/análise
9.
Food Chem ; 298: 124980, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260963

RESUMO

Effect of phlorotannins (PT) treatments on the ployhenoloxidase (PPO) activity and quality changes of Pacific white shrimp during a 16-day period of storage in ice were studied. Among seaweeds, Sargassum tenerimum had the highest amount of PT (10.00 mg phloroglucinol/g), PPO inhibitory activity (71.94%) and therefore selected for PT extraction. The shrimp treated with 5% PT (w/v) showed the least melanosis score, pH, TVB-N values and lipid oxidation among all treatments throughout the iced storage. Lower counts of mesophilic and psychrotrophic bacteria (1-2 log CFU/g) were obtained with 5% PT treatment compared to the control at the last day of storage (P < 0.05). Sensory evaluation proved that 5% PT treatment could cause a 4-days increase in the shelf-life of shrimp compared to the control, PT1% and PT2% treatments. Therefore, 5% phlorotannins from S. tenerimum could be used as a safe melanosis inhibitor for the treatment of shrimp.


Assuntos
Armazenamento de Alimentos/métodos , Penaeidae , Floroglucinol/farmacologia , Frutos do Mar , Animais , Bactérias/efeitos dos fármacos , Catecol Oxidase/antagonistas & inibidores , Catecol Oxidase/química , Catecol Oxidase/metabolismo , Microbiologia de Alimentos , Qualidade dos Alimentos , Gelo , Melanose , Oxirredução , Penaeidae/efeitos dos fármacos , Penaeidae/microbiologia , Sargassum/química , Frutos do Mar/microbiologia
10.
Food Chem ; 299: 125112, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31299521

RESUMO

The objective of this work was to assess the impact of high-pressure processing (HPP) on the carotenoid profile, colour as well as the microbial and enzymatic stability of cloudy carrot juice. The predominant carotenoids in the fresh juices were by far the provitamin A carotenoids ß-carotene and α-carotene. Others were ζ-carotene, phytofluene, phytoene and lutein. HPP at 300 MPa in three cycles caused the highest carotenoids degradation (41%) whereas the lowest degradation (26%) was achieved at 600 MPa. The highest inactivation of POD (31%) and PPO (57%) was achieved with 600 MPa and 300 MPa applied in three cycles, respectively what indicates that POD is more responsible for carotenoids degradation. The colour differences (ΔE*ab) between fresh juice and HPP-treated juices ranged from 3.02 to 4.15 CIELAB units. As far as the impact on microorganism was concerned, there was a clear trend between the applied pressure and the microbial reduction achieved.


Assuntos
Carotenoides/química , Daucus carota/química , Indústria de Processamento de Alimentos/métodos , Sucos de Frutas e Vegetais , Carotenoides/análise , Catecol Oxidase/química , Cor , Estabilidade Enzimática , Armazenamento de Alimentos , Sucos de Frutas e Vegetais/microbiologia , Pressão
11.
Food Chem ; 300: 125194, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31325749

RESUMO

The effects of near freezing temperature (NFT) storage at -1.9 °C on cell wall degradation of 'Shushanggan' apricot was studied comparing to 0 °C and 5 °C storage. Our results indicated that NFT storage strongly inhibited the solubilization of Na2CO3-soluble pectin and cellulose, by the suppression of cell wall modifying enzymes (polygalacturonase, ß-Galactosidase, pectin methyl esterase and cellulase) and related genes expressions. The loss of side chains was the main modification in CDTA (Cyclohexane-diamine-tetraacetic Acid)-soluble pectin during storage and made the main contribution to the softening of apricot, while the loss of side chain was suppressed by NFT storage. Microscopic observation showed that NFT storage delayed the degradation of pectin fraction and protected cell wall structure from loosing. This study proves that NFT storage is an effective technology to suppress the cell wall polysaccharides degradation and ultrastructure modification of apricot.


Assuntos
Parede Celular/ultraestrutura , Armazenamento de Alimentos/métodos , Polissacarídeos/química , Prunus armeniaca/química , Hidrolases de Éster Carboxílico/química , Hidrolases de Éster Carboxílico/metabolismo , Parede Celular/química , Parede Celular/metabolismo , Celulose/química , Temperatura Baixa , Congelamento , Frutas/química , Frutas/citologia , Frutas/ultraestrutura , Pectinas/química , Células Vegetais/química , Células Vegetais/ultraestrutura , Poligalacturonase/química , Poligalacturonase/metabolismo , Polissacarídeos/metabolismo , Prunus armeniaca/citologia , Solubilidade , beta-Galactosidase/química , beta-Galactosidase/metabolismo
12.
Food Chem ; 300: 125169, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31336273

RESUMO

Red drupelet is a postharvest disorder of blackberries with several drupelets turning back to red. This affects visual quality and thus marketability and consumers' acceptance. However, the cause of this disorder as well as metabolite changes during color reversion have not been fully understood. Anthocyanins, cyanidin 3-glucoside, cyanidin 3-malonylglucoside, cyanidin 3-dioxalylglucoside, and total anthocyanin, were significantly lower in red drupelets than in black drupelets after 7 days of storage. Sugars and organic acids, lipids, and free amino acids also changed with storage and by color reversion. The untargeted metabolomics analyses indicated that red drupelets were generally differentiated from berries at harvest or black drupelets at metabolite level. The results of this study help better understand the red drupelet disorder. To our knowledge, this is the first study investigating red drupelet disorder by comparing black and red drupelets at metabolite level.


Assuntos
Metabolômica/métodos , Rubus/metabolismo , Aminoácidos/análise , Aminoácidos/metabolismo , Antocianinas/análise , Antocianinas/metabolismo , Cor , Qualidade dos Alimentos , Armazenamento de Alimentos , Frutas/química , Glucosídeos/análise , Glucosídeos/metabolismo , Lipídeos/análise , Rubus/química
13.
Toxicol Lett ; 314: 75-81, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31284020

RESUMO

Aflatoxin is a fungal secondary metabolite with high toxicity that is capable of contaminating various types of food crops. It has been identified as a Group 1 human carcinogen by the International Agency for Research on Cancer. Chronic aflatoxin exposure has caused worldwide concern as a matter of public food safety. Peanuts and peanut products are the major sources of aflatoxin exposure. Therefore, some reduction interventions have been developed to minimize contamination throughout the peanut production chain. The purpose of this study is to estimate the efficacy of interventions in reducing the health impact of hepatocellular carcinoma caused by aflatoxin contamination in peanuts. The estimated total Disability-Adjusted Life Years (DALYs) were calculated using FDA-iRISK software. Six aflatoxin reduction strategies were evaluated, including good agricultural practice (GAP), biocontrol, Purdue Improved Crop Storage packaging, basic processing, ozonolysis, and ultraviolet irradiation. The results indicated that basic processing could prevent huge public health loss of 4,079.7-21,833 total DALYs per year. In addition, GAP and biocontrol were both found to be effective strategies in the farm field. Meanwhile, the other three interventions had limited effectiveness in reducing total DALYs. In conclusion, this study could help farmers, processing plants, and government policy makers to alleviate aflatoxin contamination issues in the peanut production chain.


Assuntos
Aflatoxinas/efeitos adversos , Arachis/microbiologia , Carcinoma Hepatocelular/prevenção & controle , Produtos Agrícolas/microbiologia , Exposição Dietética/efeitos adversos , Exposição Dietética/prevenção & controle , Microbiologia de Alimentos/métodos , Doenças Transmitidas por Alimentos/prevenção & controle , Neoplasias Hepáticas/prevenção & controle , Anos de Vida Ajustados por Qualidade de Vida , Agentes de Controle Biológico , Carcinoma Hepatocelular/diagnóstico , Carcinoma Hepatocelular/epidemiologia , Efeitos Psicossociais da Doença , Avaliação da Deficiência , Manipulação de Alimentos/métodos , Armazenamento de Alimentos , Doenças Transmitidas por Alimentos/diagnóstico , Doenças Transmitidas por Alimentos/epidemiologia , Humanos , Neoplasias Hepáticas/diagnóstico , Neoplasias Hepáticas/epidemiologia , Ozônio/química , Fatores de Proteção , Medição de Risco , Fatores de Risco , Fatores de Tempo , Raios Ultravioleta
14.
J Agric Food Chem ; 67(30): 8312-8318, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31287303

RESUMO

The role of inositol 1,4,5-trisphosphate (IP3) in nitric oxide (NO)-reduced chilling injury (CI) in peach fruit was investigated. The fruit were immersed in sodium nitroprusside (SNP) (NO donor) and neomycin (IP3 inhibitor). Results showed that chilling tolerance was enhanced upon exogenous SNP in postharvest peach fruit. Further, GABA accumulation was stimulated by SNP. The increase in protein expression and activity for enzymes in GABA biosynthesis, including glutamate decarboxylase (GAD), polyamine oxidase (PAO), and amino aldehyde dehydrogenase (AMADH), upon SNP treatment was also observed. Also, the up-regulation of Δ1-pyrroline-5-carboxylate synthetase (P5CS) and ornithine d-aminotransferase (OAT) and the down-regulation of proline dehydrogenase (PDH) were induced by SNP treatment, thereby accelating proline production. Additionally, SNP treatment elevated protein expression and activity of alternative oxidase (AOX). The above effects induced upon SNP were partly weakened by neomycin. Therefore, IP3 mediated NO-activated GABA and proline accumulation as well as AOX, thus inducing chilling tolerance in postharvest peach fruit.


Assuntos
Frutas/química , Inositol 1,4,5-Trifosfato/metabolismo , Óxido Nítrico/metabolismo , Prunus persica/metabolismo , Aldeído Desidrogenase/metabolismo , Temperatura Baixa , Armazenamento de Alimentos , Frutas/metabolismo , Glutamato Descarboxilase/metabolismo , Proteínas Mitocondriais/metabolismo , Oxirredutases/metabolismo , Oxirredutases atuantes sobre Doadores de Grupo CH-NH/metabolismo , Proteínas de Plantas/metabolismo , Prolina/metabolismo , Prunus persica/química , Prunus persica/enzimologia , Ácido gama-Aminobutírico/metabolismo
15.
Food Chem ; 300: 125219, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31351259

RESUMO

Although storage temperature is important for partial coalescence, the literature is scant on exploring the partial coalescence behavior between refrigerated and room temperature storage. In this study, comparison of the partial coalescence behavior between 4 and 20 °C was investigated towards corresponding oil-in-water emulsions, and subsequently towards the ultimate properties of the aerated colloidal system. As expected, compared to the value of Avrami constant (K) at 20 °C, the value of palm kernel stearin (PKS85) and mixtures of PKS85 and glycerol monostearate (PKS85-GMS) obtained at 4 °C increased by 22 and 14 times, respectively. PKS85 and PKS85-GMS displayed the needlelike appearance (N-type crystal) with a little layer crystal (L-type crystal) at 4 °C and spherical shape formed by L-type crystal along with granular crystal at 20 °C. Interestingly, several unstable air bubbles with irregularly-shape were observed in the aerated emulsions at 20 °C, while these emulsions at 4 °C displayed numerous rounded and uniform air bubbles with glossy surface. This was attributed to the sufficient stiff needle crystals at 4 °C, facilitating the coalescence of fat globules via liquid fat bridges, further forming a rigid crystal-based network and trapping the air bubbles. Therefore, our findings gained an insight into the partial coalescence behavior in emulsion, providing theoretical support for designing and optimizing the production process of foam structure products.


Assuntos
Emulsões/química , Armazenamento de Alimentos/métodos , Ar , Cristalização , Óleo de Palmeira/química , Ácidos Esteáricos/química , Temperatura Ambiente , Água/química
16.
Food Chem ; 300: 125242, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31352285

RESUMO

New antioxidant polyamide was prepared by total immersion in active extract for 48 h. Its antioxidant performance was tested using DPPH (IC50 = 270 ±â€¯21 µg/g) and ORAC (1.52 µmol Trolox/g). In vivo study was done using fresh minced meat stored at 4 °C and analysed after 0, 6, 13, 19, 23 days. After 23 days metmyoglobin (MetMbBK = 31.3 ±â€¯2.9% and MetMbAOX = 25.9 ±â€¯0.8%), CIE L*a*b* (a*BK = 15.0 ±â€¯0.4 and a*AOX = 16.6 ±â€¯0.3) and TBARS (MDABK = 0.0060 ±â€¯0.0003 µg/g and MDAAOX = 0.0044 ±â€¯0.0002 µg/g) showed an improvement of meat shelf life. The results showed that this active film protected meat during 23 days. Migration study to food simulants was done by UPLC®-QqQ-MS and UPLC®-ESI-Q-TOF-MSE. A decrease of oligomers migration (for caprolactam n = 3: BK = 0.050 ±â€¯0.004 mg/Kg; AOX = 0.019 ±â€¯0.001 mg/Kg) was found. INDUSTRIAL RELEVANCE: Nowadays, food industry is focused on improving shelf life of products by controlling its lipid oxidation using natural antioxidants. The obtained results let us design a new active packaging based on natural antioxidants for extending the shelf life of fresh minced meat at industrial scale.


Assuntos
Antioxidantes/química , Embalagem de Alimentos/métodos , Carne , Nylons/química , Chá/química , Animais , Antioxidantes/farmacologia , Cromatografia Líquida de Alta Pressão , Conservação de Alimentos/métodos , Armazenamento de Alimentos/métodos , Metabolismo dos Lipídeos , Carne/análise , Metamioglobina/análise , Nylons/farmacologia , Oxirredução , Espectrometria de Massas por Ionização por Electrospray , Substâncias Reativas com Ácido Tiobarbitúrico/análise
17.
Food Chem ; 300: 125249, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31352291

RESUMO

Under the optimal conditions, a crosslinked electrospun polyvinyl alcohol/cinnamon essential oil/ß-cyclodextrin (CPVA-CEO-ß-CD) nanofibrous films for sustained release of antimicrobials were successfully prepared. Cinnamon essential oil (CEO) can be sustainably released due to CPVA-CEO-ß-CD nanofibers complex delivery systems. The chemical crosslinking and physical welding achieved simultaneously by glutaraldehyde atomization fumigation, making fibers more suitable for fresh food packaging. Nanofibrous films were characterized in terms of SEM, ATR-FTIR, DSC, water contact angle analysis and antibacterial trials. ATR-FTIR and DSC data indicated that CEO was encapsulated in a ß-CD cavity and they coexisted in PVA nanofibers. The water contact angle of the crosslinked PVA nanofibrous films increased with CEO and the values were always below 90°. Crosslinked nanofibers possessed fine properties in vitro antibacterial against Staphylococcus aureus and Escherichia coli. Furthermore, CPVA/ß-CD/CEO nanofibrous films delayed decay of mushroom during storage, indicating their potential implementation in active food packaging.


Assuntos
Agaricales/fisiologia , Embalagem de Alimentos/métodos , Nanofibras/química , Álcool de Polivinil/química , beta-Ciclodextrinas/química , Antibacterianos/química , Antibacterianos/farmacologia , Cinnamomum zeylanicum/química , Reagentes para Ligações Cruzadas/química , Escherichia coli/efeitos dos fármacos , Embalagem de Alimentos/instrumentação , Armazenamento de Alimentos , Glutaral/química , Microscopia Eletrônica de Varredura , Óleos Voláteis/química , Espectroscopia de Infravermelho com Transformada de Fourier , Staphylococcus aureus/efeitos dos fármacos
18.
Food Chem ; 299: 125163, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31319344

RESUMO

Citrus fruit postharvest degreening is a critical stage in marketing, carried out by exposure to ethylene or ethephon. Genome-wide screening of the AP2/ERF superfamily indicated that a novel ERF-II (CitERF6) was shown to trans-activate the CitPPH promoter. Expression of CitERF6 is associated with both developmental and postharvest degreening in citrus fruit. Transient and stable over-expression of CitERF6 in Nicotiana tabacum leaves and 'Ponkan' fruit also results in rapid chlorophyll degradation. Auto- and mutual-regulation was also found between CitERF6 and the previously characterized CitERF13 using the dual-luciferase and yeast one-hybrid assays. Moreover, substitution of the 35S promoter for endogenous promoters showed that both pCitERF6::CitERF6 and pCitERF13::CitERF13 were effective in trans-activating their promoters or triggering chlorophyll degradation. It is proposed that ethylene is one of the triggers activating promoters of CitERF6 and CitERF13, and subsequent auto- and mutual-regulation between CitERF6 and CitERF13 might facilitate the effect of ethylene, leading to fruit degreening.


Assuntos
Citrus/fisiologia , Etilenos/metabolismo , Frutas/fisiologia , Proteínas de Plantas/metabolismo , Clorofila/genética , Clorofila/metabolismo , Armazenamento de Alimentos , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas , Regiões Promotoras Genéticas , Tabaco/genética
19.
Food Chem ; 299: 125132, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31299519

RESUMO

Protein oxidation of beef patties stored in high oxygen modified atmosphere packaging for 9 days was investigated. Meat was either stored in the dark, under light, or in the dark with addition of FeCl2/H2O2/myoglobin (forced oxidation). SDS-PAGE analysis showed high degree of protein polymerization for meat exposed to light, compared to the other samples. Light exposure induced reducible (disulfide) and non-reducible cross-links, while mainly disulfides were formed in meat stored in the dark. Light exposure was responsible for 58% loss of free thiols (Cys residues). No significant loss of other amino acid residues was observed and none of the most common oxidation products of tryptophan, tyrosine, and phenylalanine were detected. Intrinsic fluorescence measurements of tryptophan showed 27% loss in samples exposed to light, which was ascribed to loss of protein solubility via protein polymerization rather than tryptophan oxidation. Protein carbonyls were mainly detected in forced oxidized samples at Day 0.


Assuntos
Armazenamento de Alimentos/métodos , Proteínas de Carne/química , Carne Vermelha , Animais , Bovinos , Dissulfetos/química , Embalagem de Alimentos , Peróxido de Hidrogênio/química , Luz , Mioglobina/química , Oxirredução , Oxigênio , Polimerização , Carne Vermelha/análise , Carne Vermelha/efeitos da radiação , Compostos de Sulfidrila/química , Triptofano/química , Tirosina/química , Raios Ultravioleta
20.
Food Chem ; 299: 125138, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31302430

RESUMO

As an environmentally friendly approach for fruit quality improvement, the effect of preharvest UV-C on the physiology of strawberry fruit during postharvest storage remains to be assessed. Strawberry fruit developed with supplementary UV-C were stored at room temperature for 2 weeks. Preharvest UV-C attenuated fruit postharvest senescence and altered phytochemicals composition. Higher ester titer was found in the treated fruit at harvest, whereas higher terpene and furanone contents were detected after 72 h of storage. At harvest, polyphenolics accumulated to a higher level in UV-C group, but the difference disappeared after 24 h of storage. Meanwhile, the intrinsic level of abscisic acid and the expressions of FaPYR1, SnRK2, and FaASR in the UV-C-treated fruit was enhanced at harvest but returned to a lower level as storage proceeded. This study highlights the time-dependent effect of preharvest UV-C on strawberry fruit postharvest biochemical indexes and the possible involvement of abscisic acid signaling factors.


Assuntos
Ácido Abscísico/metabolismo , Armazenamento de Alimentos/métodos , Fragaria/fisiologia , Frutas/fisiologia , Compostos Fitoquímicos/metabolismo , Ácido Abscísico/genética , Fragaria/química , Fragaria/efeitos da radiação , Frutas/química , Frutas/metabolismo , Frutas/efeitos da radiação , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Compostos Fitoquímicos/análise , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Polifenóis/análise , Polifenóis/metabolismo , Terpenos/análise , Terpenos/metabolismo , Raios Ultravioleta
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA