Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 121
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Int J Mol Sci ; 22(16)2021 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-34445327

RESUMO

The fight against cancer is one of the main challenges for medical research. Recently, nanotechnology has made significant progress, providing possibilities for developing innovative nanomaterials to overcome the common limitations of current therapies. In this context, silver nanoparticles (AgNPs) represent a promising nano-tool able to offer interesting applications for cancer research. Following this path, we combined the silver proprieties with Artemisia arborescens characteristics, producing novel nanoparticles called Artemisia-AgNPs. A "green" synthesis method was performed to produce Artemisia-AgNPs, using Artemisia arborescens extracts. This kind of photosynthesis is an eco-friendly, inexpensive, and fast approach. Moreover, the bioorganic molecules of plant extracts improved the biocompatibility and efficacy of Artemisia-AgNPs. The Artemisia-AgNPs were fully characterized and tested to compare their effects on various cancer cell lines, in particular HeLa and MCF-7. Artemisia-AgNPs treatment showed dose-dependent growth inhibition of cancer cells. Moreover, we evaluated their impact on the cell cycle, observing a G1 arrest mediated by Artemisia-AgNPs treatment. Using a clonogenic assay after treatment, we observed a complete lack of cell colonies, which demonstrated cell reproducibility death. To have a broader overview on gene expression impact, we performed RNA-sequencing, which demonstrated the potential of Artemisia-AgNPs as a suitable candidate tool in cancer research.


Assuntos
Antineoplásicos , Apoptose/efeitos dos fármacos , Artemisia/química , Nanopartículas Metálicas , Prata , Antineoplásicos/síntese química , Antineoplásicos/química , Antineoplásicos/uso terapêutico , Apoptose/genética , Artemisia/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/genética , Ensaios de Seleção de Medicamentos Antitumorais , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Química Verde , Células HeLa , Humanos , Células MCF-7 , Nanopartículas Metálicas/química , Nanopartículas Metálicas/uso terapêutico , Células PC-3 , Extratos Vegetais/química , Prata/química , Prata/uso terapêutico
2.
Ecotoxicol Environ Saf ; 223: 112566, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34340153

RESUMO

Artemisia ordosica is one of the main shrubby perennials belonging to Artemisia species of Asteraceae and could be used in folk Chinese/Mongolian medicine to treat symptoms of various inflammatory ailments. The present study was conducted to investigate the protective effects of dietary Artemisia ordosica polysaccharide (AOP) against lipopolysaccharide (LPS) induced oxidative stress in broilers via Nrf2/Keap1 and TLR4/NF-κB pathway. A total of 192 1-day-old Arbor Acres male broilers were randomly allotted to four treatments with 6 replicates (n = 8): (1) CON group, non-challenged broilers fed basal diet; (2) LPS group, LPS-challenged broilers fed basal diet; (3) AOP group, non-challenged broilers fed basal diet supplemented with 750 mg/kg AOP; (4) LPS+AOP group, LPS-challenged broilers fed basal diet supplemented with 750 mg/kg AOP. The trial included starter phase (d 1-14), stress period Ⅰ (d 15-21), convalescence Ⅰ (d 22-28), stress period Ⅱ (d 29-35) and convalescence Ⅱ (d 36-42). During stress period Ⅰ (on d 15, 17, 19 and 21) and stress period Ⅱ (on d 29, 31, 33 and 35), broilers were injected intra-abdominally either with LPS solution or with an equal amount of sterile saline. The results showed that dietary AOP supplementation alleviated LPS-induced reduction in antioxidant enzyme activity and excessive production of ROS, 8-OHdG and PC in serum of broilers challenged with LPS. Moreover, dietary AOP supplementation alleviated the decrease of T-AOC and activities of SOD, CAT and GPx in liver of broilers challenged with LPS by increasing expression of Nrf2, and inhibiting over-expression of Keap1 both at gene and protein level. Additionally, dietary AOP supplementation decreased the over-production of IL-1ß and IL-6 in liver of broilers challenged by LPS through decreasing mRNA expression of TLR4, MyD88, NF-κB P65, IL-1ß and IL-6, and alleviating the increase of protein expression of TLR4, IKKß, NF-κB P65, IL-1ß, IL-6, and the decrease of protein expression of IkBα. In conclusion, dietary AOP supplementation could alleviate LPS-induced oxidative stress through Nrf2/Keap1 and TLR4/NF-κB pathway.


Assuntos
Artemisia , Lipopolissacarídeos , Ração Animal/análise , Animais , Artemisia/metabolismo , Galinhas/metabolismo , Dieta , Suplementos Nutricionais , Proteína 1 Associada a ECH Semelhante a Kelch/genética , Lipopolissacarídeos/toxicidade , Masculino , Fator 2 Relacionado a NF-E2/genética , NF-kappa B/genética , NF-kappa B/metabolismo , Estresse Oxidativo , Polissacarídeos , Receptor 4 Toll-Like/genética
3.
Molecules ; 26(12)2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-34203927

RESUMO

Artemisia ludoviciana subsp. mexicana has been traditionally used for the treatment of digestive ailments such as gastritis, whose main etiological agent is Helicobacter pylori. In a previous screening study, the aqueous extract exhibited a good in vitro anti-H. pylori activity. With the aim of determining the efficacy of this species as a treatment for H. pylori related diseases and finding bioactive compounds, its aqueous extract was subjected to solvent partitioning and the fractions obtained were tested for their in vitro anti-H. pylori effect, as well as for their in vivo gastroprotective and anti-inflammatory activities. The aqueous extract showed a MIC = 250 µg/mL. No acute toxicity was induced in mice. A gastroprotection of 69.8 ± 3.8%, as well as anti-inflammatory effects of 47.6 ± 12.4% and 38.8 ± 10.2% (by oral and topical administration, respectively), were attained. Estafiatin and eupatilin were isolated and exhibited anti-H. pylori activity with MBCs of 15.6 and 31.2 µg/mL, respectively. The finding that A. ludoviciana aqueous extract has significant anti-H. pylori, gastroprotective and anti-inflammatory activities is a relevant contribution to the ethnopharmacological knowledge of this species. This work is the first report about the in vivo gastroprotective activity of A. ludoviciana and the anti-H. pylori activity of eupatilin and estafiatin.


Assuntos
Artemisia/metabolismo , Flavonoides/farmacologia , Helicobacter pylori/efeitos dos fármacos , Animais , Antiulcerosos/farmacologia , Flavonoides/metabolismo , Gastrite/tratamento farmacológico , Masculino , Medicina Tradicional , Camundongos , Camundongos Endogâmicos , Extratos Vegetais/farmacologia , Sesquiterpenos/metabolismo , Sesquiterpenos/farmacologia , Úlcera Gástrica/tratamento farmacológico
4.
Molecules ; 26(10)2021 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-34065533

RESUMO

The Artemisia genus includes a large number of species with worldwide distribution and diverse chemical composition. The secondary metabolites of Artemisia species have numerous applications in the health, cosmetics, and food sectors. Moreover, many compounds of this genus are known for their antimicrobial, insecticidal, parasiticidal, and phytotoxic properties, which recommend them as possible biological control agents against plant pests. This paper aims to evaluate the latest available information related to the pesticidal properties of Artemisia compounds and extracts and their potential use in crop protection. Another aspect discussed in this review is the use of nanotechnology as a valuable trend for obtaining pesticides. Nanoparticles, nanoemulsions, and nanocapsules represent a more efficient method of biopesticide delivery with increased stability and potency, reduced toxicity, and extended duration of action. Given the negative impact of synthetic pesticides on human health and on the environment, Artemisia-derived biopesticides and their nanoformulations emerge as promising ecofriendly alternatives to pest management.


Assuntos
Artemisia/metabolismo , Nanotecnologia , Controle Biológico de Vetores/métodos , Praguicidas/metabolismo , Produtos Agrícolas/microbiologia
5.
Chem Biodivers ; 18(8): e2100241, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34081830

RESUMO

Artemisia fragrans is commonly used as a folk medicine as antispasmodic, anti-pyretic, anti-inflammatory, and abortifacient agents. The villagers use its pungent odor to repel rodents, mites, and pests, as well as its essential oil and smoke after burning to treat lung infections after uprooting the plant. Herein, we extracted the essential oils (EOs) of different parts of the plant and analyzed their chemical compositions and antibacterial activities. The chemical analysis led to the identification of 73, 59, and 57 compounds in the EOs of the stem, leaf, and flower, respectively. All of the EOs exhibited antibacterial activities against both G+ and G- bacteria. The EOs of the leaf and flower were more effective against tested bacteria, except B. anthracis and P. aeruginosa, compared to that of the stem. The binary combination of the EOs (stem and flower) or (stem and leaf) showed a synergistic effect. Statistical analysis indicated EOs of leaf and flower are more potent than that of the stem. These findings suggest the application of leaf and flower of the plant, which not only can prevent its uprooting but also ensure better therapeutic function.


Assuntos
Anti-Infecciosos/química , Artemisia/química , Óleos Voláteis/química , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Artemisia/metabolismo , Flores/química , Flores/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Medicina Tradicional , Testes de Sensibilidade Microbiana , Óleos Voláteis/farmacologia , Folhas de Planta/química , Folhas de Planta/metabolismo , Caules de Planta/química , Caules de Planta/metabolismo , Terpenos/química , Terpenos/isolamento & purificação , Terpenos/farmacologia
6.
Ecotoxicol Environ Saf ; 220: 112315, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34015628

RESUMO

Particulate organic matter (POM) is an effective adsorbent for decreasing the contaminant of cadmium, but little is known about the relevant mechanisms under the effect of plant. In this work, POM were used to study the removal of Cd2+ in the initial concentration range of 0-4.46 mmol L-1 at pH 5.5, and the effect of Artemisia ordosica roots and pH on kinetics and equilibrium of cadmium adsorption on POM and soils were examined. The result indicated that adsorption kinetics fit well with the pseudo-second-order kinetic model, and the equilibrium data for Cd adsorption fit much well to the Langmuir model. The maximum adsorption capacity for POM at equilibrium corresponding to the monolayer coverage reached 0.287 mmol/g for Cd. The amount of Cd adsorbed in the POM and soil increased with the increase of pH from 4 to 8.5. The Artemisia ordosica roots decreased Cd adsorption in POM; instead, the adsorption capacity of soil for Cd was improved under the effect of Artemisia ordosica roots. The Fourier Transform Infrared spectroscopic (FTIR) analysis indicated that the complexation of POM and Cd was mainly through sulfhydryl, hydroxyl and carboxyl groups.


Assuntos
Artemisia/metabolismo , Cádmio/metabolismo , Material Particulado/química , Raízes de Plantas/metabolismo , Poluentes do Solo/química , Poluentes do Solo/metabolismo , Solo/química , Adsorção , Compostos Orgânicos/química
7.
Chem Pharm Bull (Tokyo) ; 69(4): 411-413, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33518581

RESUMO

Due to high need for medical purposes, multiple harvests of mugwort (Artemisia argyi) have been extensively applied in China for the increase of mugwort yield recently. However, the investigation on the mineral elements in different crops, which are significantly related to mugwort growth and the clinical efficacy of this medicinal herb, has not been conducted. This study provided an analytical method and quality evaluation for mineral elements in Nanyang mugwort leaves harvested from three different crops. The contents of 35 mineral elements were determined by inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma atomic emission spectrometry (ICP-AES). ANOVA, principal component analysis and factor analysis were applied to evaluate the results. Four principal components were identified and their comprehensive evaluation function was as follows: F = 0.7008Fl + 0.1236F2 + 0.0936F3 + 0.0321F4. The comprehensive scores of the mugwort leaves from different crops were ranked as follows: 3rd crop > 2nd crop ≈ 1st crop. These findings can provide a reference for the quality control and clinical use of mugwort leaves, and a guidance of differential nourishing strategies for different crops.


Assuntos
Artemisia/química , Espectrometria de Massas , Minerais/análise , Espectrofotometria Atômica , Artemisia/metabolismo , Medicamentos de Ervas Chinesas/análise , Folhas de Planta/química , Folhas de Planta/metabolismo , Análise de Componente Principal
8.
Int J Biol Macromol ; 172: 263-269, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33453254

RESUMO

In insects, the cytochrome P450 CYP6B family plays key roles in the detoxification of toxic plant substances. However, the function of CYP6 family genes in degrading plant toxicants in Tribolium castaneum, an extremely destructive global storage pest, have yet to be elucidated. In this study, a T. castaneum CYP gene, TcCYP6BQ7, was characterized. TcCYP6BQ7 expression was significantly induced after exposure to essential oil of the plant Artemisia vulgaris (EOAV). Spatiotemporal expression profiling revealed that TcCYP6BQ7 expression was higher in larval and adult stages of T. castaneum than in other developmental stages, and that TcCYP6BQ7 was predominantly expressed in the brain and hemolymph from the late larval stage. TcCYP6BQ7 silencing by RNA interference increased larvae mortality in response to EOAV from 49.67% to 71.67%, suggesting that this gene is associated with plant toxicant detoxification. Combined results from this study indicate that the CYP6 family gene TcCYP6BQ7 likely plays a pivotal role in influencing the susceptibility of T. castaneum to plant toxicants. These findings may have implications for the development of novel therapeutics to control this agriculturally important pest.


Assuntos
Sistema Enzimático do Citocromo P-450/genética , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Óleos Voláteis/farmacologia , Proteínas de Plantas/genética , Pupa/efeitos dos fármacos , Tribolium/efeitos dos fármacos , Animais , Artemisia/química , Artemisia/metabolismo , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Sistema Enzimático do Citocromo P-450/metabolismo , Feminino , Regulação da Expressão Gênica , Hemolinfa/efeitos dos fármacos , Hemolinfa/metabolismo , Inseticidas/isolamento & purificação , Inseticidas/metabolismo , Larva/genética , Larva/crescimento & desenvolvimento , Larva/metabolismo , Longevidade/efeitos dos fármacos , Longevidade/genética , Masculino , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/metabolismo , Proteínas de Plantas/antagonistas & inibidores , Proteínas de Plantas/metabolismo , Pupa/genética , Pupa/crescimento & desenvolvimento , Pupa/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Tribolium/genética , Tribolium/crescimento & desenvolvimento , Tribolium/metabolismo
9.
Nat Prod Res ; 35(1): 152-156, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31135230

RESUMO

The importance of the present study is to find out the phytochemical profile of essential oil (EO) of Artemisia tournefortiana Reichb. and its biological evaluation. Nineteen volatile constituents were identified from EO representing 93.47% of total oil composition. Oxygenated monoterpenes (54.46%) were found to be dominant over other class of compounds. cis-spiroether (47.66%), Z-ß-farnesene (22.83%), trans-nerolidol (3.89%) and camphor (3.80%) were found to be the major constituents. cis-spiroether is first time identified in this region. Antibacterial effects were observed against both gram-positive and gram-negative bacteria with maximum zone of inhibition (32 mm) against Staphylococcus aureus and minimum inhibitory concentration (MIC) of all tested strains were found in the range of 1.6-3.4 µg/mL. Hence the antimicrobial effect of this plant EO obtained from the natural source could be utilised in order to overcome the problem of microbial drug resistance. The EO showed moderate antioxidant effect through DPPH assay with IC50 value of 56.2 µg/mL. The significant antioxidant activity can be attributed to the presence of various conjugated secondary metabolites, phenolics and hydroxyl group bearing constituents present in the essential oil.[Figure: see text].


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Artemisia/química , Óleos Voláteis/farmacologia , Compostos Orgânicos Voláteis/análise , Antibacterianos/química , Antioxidantes/química , Artemisia/metabolismo , Cânfora/análise , Avaliação Pré-Clínica de Medicamentos , Cromatografia Gasosa-Espectrometria de Massas , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Índia , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Monoterpenos/análise , Monoterpenos/química , Óleos Voláteis/análise , Óleos Voláteis/química , Sesquiterpenos/análise , Sesquiterpenos/química , Staphylococcus aureus/efeitos dos fármacos
10.
Nat Prod Res ; 35(12): 1959-1967, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31364880

RESUMO

Artemisia herba-alba is widely used in traditional medicines for the treatment of several diseases. From the aerial parts organic extract of A. herba-alba, two new compounds, 1,3,8-trihydroxyeudesm-4-en-7α,11ßH-12,6α-olide (1) and 5-ß-​D-​glucopyranosyloxy​-​7-methoxy-​6H-​benzopyran-​2-​one (2), respectively, together with five known metabolites: 3α,8ß-dihydroxygermacr-4(15),9(10)-dien-7ß,11αH,12,6α-olide (3), 1ß,8α-dihydroxy-11α,13-dihydrobalchanin (4), 11-epiartapshin (5), tomenin (6) and benzoic acid, p-​(ß-​D-​glucopyranosyloxy)​-​methyl ester (7), were isolated and identified. The chemical structures were proven depending upon spectroscopic analysis, including 1 D/2D NMR as and ESI-MS. Compound 1 inhibited Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus. Compounds 2 and 3 exhibited antibacterial activity against both gram-positive and gram-negative bacteria.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Artemisia/metabolismo , Artemisia/química , Bacillus subtilis/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Bactérias Gram-Negativas/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/química , Plantas Medicinais/química , Plantas Medicinais/metabolismo , Espectrometria de Massas por Ionização por Electrospray , Staphylococcus aureus/efeitos dos fármacos
11.
Carbohydr Polym ; 251: 117044, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33142602

RESUMO

Seed mucilage has significant economic value. However, the identification of key regulatory genes in mucilage formation and their molecular regulatory mechanism remain unknown. Artemisia sphaerocephala seeds are rich in mucilage. In this study, A. sphaerocephala seeds in 10, 20, 30, 40, 50, 60 and 70 days after flowering were used as materials to reveal their molecular regulatory mechanism in mucilage formation by RNA-sequencing and weighted gene co-expression network analysis (WGCNA). 21 key regulatory genes for mucilage formation were identified, including AsKNAT7 and AsTTG1 genes, as well as AsNAM and AsAP2 gene families. From 10-30 days after flowering, both AsNAM and AsAP2 supported mucilage formation. From 40-70 days after flowering, promotion by AsNAM and AsAP2 was weakened and the up-regulation of AsKNAT7 inhibited mucilage formation, leading to no further increases in mucilage content. This in depth elucidation of seed mucilage formation lays the foundation for the application of mucilage.


Assuntos
Artemisia/crescimento & desenvolvimento , Artemisia/genética , Mucilagem Vegetal/biossíntese , Polissacarídeos/biossíntese , Adaptação Fisiológica , Artemisia/metabolismo , Regulação da Expressão Gênica de Plantas , Germinação , Mucilagem Vegetal/genética , Polissacarídeos/genética , Sementes/genética , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Transcriptoma
12.
Nat Prod Res ; 34(21): 3061-3065, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31084212

RESUMO

Two new compounds, named ordosacid A (5) and ordosacid B (6), along with four known compounds: 3,4-dihydroxybenzaldehyde (1), p-hydroxybenzoic acid (2), p-hydroxycinnamic acid (3) and o-hydroxycinnamic acid (4), were isolated from the ethyl acetate extract of Artemisia ordosica Krasch. The structures of new compounds were elucidated on the basis of spectroscopic methods including UV, IR, ESI-MS, 1D NMR, 2D NMR, HR-ESI-MS and modified Mosher's method.


Assuntos
Artemisia/química , Produtos Biológicos/química , Artemisia/metabolismo , Produtos Biológicos/isolamento & purificação , China , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Parabenos/química , Parabenos/isolamento & purificação , Extratos Vegetais/química , Propionatos/química , Propionatos/isolamento & purificação , Metabolismo Secundário , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria Ultravioleta
13.
New Phytol ; 225(6): 2251-2266, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31651036

RESUMO

Glandular trichomes are epidermal outgrowths that are the site of biosynthesis and storage of large quantities of specialized metabolites. Besides their role in the protection of plants against biotic and abiotic stresses, they have attracted interest owing to the importance of the compounds they produce for human use; for example, as pharmaceuticals, flavor and fragrance ingredients, or pesticides. Here, we review what novel concepts investigations on glandular trichomes have brought to the field of specialized metabolism, particularly with respect to chemical and enzymatic diversity. Furthermore, the next challenges in the field are understanding the metabolic network underlying the high productivity of glandular trichomes and the transport and storage of metabolites. Another emerging area is the development of glandular trichomes. Studies in some model species, essentially tomato, tobacco, and Artemisia, are now providing the first molecular clues, but many open questions remain: How is the distribution and density of different trichome types on the leaf surface controlled? When is the decision for an epidermal cell to differentiate into one type of trichome or another taken? Recent advances in gene editing make it now possible to address these questions and promise exciting discoveries in the near future.


Assuntos
Genes de Plantas , Lycopersicon esculentum , Redes e Vias Metabólicas , Folhas de Planta/metabolismo , Proteínas de Plantas , Tabaco , Tricomas/metabolismo , Artemisia/genética , Artemisia/metabolismo , Flavonoides/metabolismo , Humanos , Lycopersicon esculentum/genética , Lycopersicon esculentum/metabolismo , Modelos Biológicos , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Metabolismo Secundário , Terpenos/metabolismo , Tabaco/genética , Tabaco/metabolismo , Tricomas/crescimento & desenvolvimento
14.
BMC Plant Biol ; 19(1): 481, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703625

RESUMO

BACKGROUND: Linoleic acid is an important polyunsaturated fatty acid, required for all eukaryotes. Microsomal delta-12 (Δ12) oleate desaturase (FAD2) is a key enzyme for linoleic acid biosynthesis. Desert shrub Artemisia sphaerocephala is rich in linoleic acid, it has a large FAD2 gene family with twenty-six members. The aim of this work is to unveil the difference and potentially functionality of AsFAD2 family members. RESULTS: Full-length cDNAs of twenty-one AsFAD2 genes were obtained from A. sphaerocephala. The putative polypeptides encoded by AsFAD2 family genes showed a high level of sequence similarity and were relatively conserved during evolution. The motif composition was also relatively conservative. Quantitative real-time PCR analysis revealed that the AsFAD2-1 gene was strongly expressed in developing seeds, which may be closely associated with the high accumulating ability of linoleic acid in A. sphaerocephala seeds. Although different AsFAD2 family members showed diverse response to salt stress, the overall mRNA levels of the AsFAD2 family genes was stable. Transient expression of AsFAD2 genes in the Nicotiana benthamiana leaves revealed that the encoded proteins were all located in the endoplasmic reticulum. Heterologous expression in Saccharomyces cerevisiae suggested that only three AsFAD2 enzymes, AsFAD2-1, - 10, and - 23, were Δ12 oleate desaturases, which could convert oleic acid to linoleic acid, whereas AsFAD2-1 and AsFAD2-10 could also produce palmitolinoleic acid. CONCLUSIONS: This research reported the cloning, expression studies, subcellular localization and functional identification of the large AsFAD2 gene family. These results should be helpful in understanding fatty acid biosynthesis in A. sphaerocephala, and has the potential to be applied in the study of plant fatty acids traits.


Assuntos
Artemisia/genética , Ácidos Graxos Dessaturases/genética , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Estresse Salino/genética , Artemisia/enzimologia , Artemisia/metabolismo , Ácidos Graxos Dessaturases/metabolismo , Perfilação da Expressão Gênica , Genes de Plantas , Família Multigênica , Filogenia , Proteínas de Plantas/metabolismo
15.
Mol Immunol ; 116: 140-150, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31654938

RESUMO

BACKGROUND: Non-specific lipid transfer proteins (LTPs) are important allergens in fruits, pollen, vegetables, nuts and latex. Due to their compact structure, LTPs are highly resistant to heat treatment. Here, Art v 3 from mugwort pollen and Pru p 3 from peach were used as model allergens to in-depth investigate structural and immunological properties upon thermal treatment at different buffer conditions. METHODS: Recombinant Art v 3 and Pru p 3 were purified from E. coli and incubated at 95 °C up to 120 min using sodium phosphate buffer pH 3.4 or 7.3. Physicochemical properties of allergens were analyzed in circular dichroism spectroscopy, Fourier transform infrared spectroscopy, dynamic light scattering, size exclusion chromatography, and mass spectrometry. The crystal structure of Art v 3.0201 was determined to 1.9 Šresolution. IgG and IgE binding was investigated in ELISA using murine and LTP allergic patients' sera. RESULTS: Highly pure and homogenous recombinant allergens were obtained from bacterial production. The crystal structure of Art v 3.0201 revealed an antiparallel four helix bundle with a C-terminal extension mediating an asymmetric, transient dimer interface and differently sized cavities. Both allergens showed high thermal stability at acidic conditions. In contrast, extensive heat treatment in neutral buffer induced irreversible structural changes due to lanthionine-based cysteine rearrangement. This fostered loss of the typical α-helical structure, increased molecular size and abrogation of IgG and IgE binding epitopes. Pru p 3 lost its structural integrity at shorter heat stress duration than Art v 3, which did however only partially affect the molecule's IgE binding epitopes. CONCLUSION: During thermal treatment, susceptibility to structural changes of the LTP-fold is highly dependent on the surrounding environment but also on intrinsic features of individual LTPs. This is a crucial fact to consider when processing LTP-containing food or food products as this will directly influence their allergenic potential.


Assuntos
Alanina/análogos & derivados , Antígenos de Plantas/metabolismo , Proteínas de Transporte/metabolismo , Cisteína/metabolismo , Proteínas de Plantas/metabolismo , Sulfetos/metabolismo , Alanina/metabolismo , Sequência de Aminoácidos , Animais , Artemisia/metabolismo , Reações Cruzadas/fisiologia , Epitopos/metabolismo , Escherichia coli/metabolismo , Hipersensibilidade Alimentar/metabolismo , Humanos , Imunoglobulina E/metabolismo , Imunoglobulina G/metabolismo , Camundongos , Pólen/metabolismo , Prunus/metabolismo
16.
J Food Biochem ; 43(7): e12855, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31353702

RESUMO

This study was performed to investigate the effects of Artemisia argyi and 4,5-dicaffeyolquinic acid (4,5-diCQA) as a main compound of ethyl acetate fraction from Artemisia argyi (EFAA) on high-fat diet (HFD)-induced cognitive dysfunction. Both EFAA and 4,5-diCQA were effective in improving cognitive function on HFD-induced cognitive dysfunction. In brain tissue analysis, it was confirmed that EFAA and 4,5-diCQA inhibited the reduction of neurotransmitters as well as oxidative stress and mitochondrial dysfunction. In addition, they inhibited amyloid ß (Aß) accumulation by increasing the expression of insulin-degrading enzyme and consequently prevented apoptosis. In conclusion, it is presumed that Artemisia argyi may help to improve the cognitive impairment due to the HFD, and it is considered that this effect is closely related to the physiological activity of 4,5-diCQA. PRACTICAL APPLICATIONS: Artemisia argyi is used in traditional herbal medicine in Asia. Type 2 diabetes mellitus has been proven by a variety of epidemiological studies to be a risk factor for cognitive impairment, such as Alzheimer's disease. This study confirmed that 4,5-diCQA is a bioactive compound of Artemisia argyi on improving HFD-induced cognitive dysfunction. Therefore, this study can provide useful information to the effect of Artemisia argyi and related substance.


Assuntos
Artemisia , Disfunção Cognitiva/tratamento farmacológico , Insulisina/efeitos dos fármacos , Extratos Vegetais/farmacologia , Ácido Quínico/análogos & derivados , Peptídeos beta-Amiloides/efeitos dos fármacos , Peptídeos beta-Amiloides/metabolismo , Apoptose/efeitos dos fármacos , Artemisia/química , Artemisia/metabolismo , Encéfalo/efeitos dos fármacos , Encéfalo/fisiopatologia , Dieta Hiperlipídica/efeitos adversos , Insulisina/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Fitoterapia , Plantas Medicinais/metabolismo , Ácido Quínico/farmacologia
17.
Phytochemistry ; 164: 144-153, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31151061

RESUMO

Triquinane is a type of sesquiterpenoid with a unique structure that contains a fused tricyclopentane ring and exhibits a wide range of bioactivities. Like other sesquiterpenoids, the first committed step in triquinane-type sesquiterpenoid biosynthesis is the cyclization of farnesyl pyrophosphate (FPP), a common precursor of all sesquiterpenoids, catalyzed by sesquiterpene synthase. Artemisia abrotanum L. (Asteraceae), a common plant used in the culinary and cosmetics industries, has been reported to accumulate high levels of triquinane silphiperfol-5-en-3-one A. This compound is potentially biosynthesized from the cyclization of FPP into 7-epi-silphiperfol-5-ene followed by a multi-step oxidation to silphiperfol-5-en-3-one A. In this study, we aimed to identify the sesquiterpene synthase responsible for the synthesis of 7-epi-silphiperfol-5-ene. We performed RNA sequencing of A. abrotanum leaves and gene candidates were mined by homology searches using the triquinane α-isocomene synthase of chamomile (MrTPS2) as query. After gene cloning, we obtained five variants of putative sesquiterpene synthase showing greater than 85% amino acid identity to MrTPS2 and greater than 95% amino acid identity to each other. Heterologous expression of these variants in a FPP-high-producing yeast strain revealed the first four variants to be (+)-α-bisabolol synthases (AabrBOS1-4). However, the fifth candidate cyclized FPP into 7-epi-silphiperfol-5-ene and can therefore be defined as a 7-epi-silphiperfol-5-ene synthase (AabrSPS). These findings revealed the first committed enzyme involved in silphiperfol-5-en-3-one A and (+)-α-bisabolol biosyntheses in A. abrotanum. Furthermore, the results of this study will be useful for enhancing the production of these compounds for further applications.


Assuntos
Alquil e Aril Transferases/análise , Artemisia/química , Artemisia/enzimologia , Sesquiterpenos/análise , Alquil e Aril Transferases/metabolismo , Artemisia/metabolismo , Relação Dose-Resposta a Droga , Conformação Molecular , Sesquiterpenos Monocíclicos , Sesquiterpenos/metabolismo , Relação Estrutura-Atividade
18.
Genome Biol Evol ; 11(7): 2023-2034, 2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-31135036

RESUMO

Sage-grouse are two closely related iconic species of the North American West, with historically broad distributions across sagebrush-steppe habitat. Both species are dietary specialists on sagebrush during winter, with presumed adaptations to tolerate the high concentrations of toxic secondary metabolites that function as plant chemical defenses. Marked range contraction and declining population sizes since European settlement have motivated efforts to identify distinct population genetic variation, particularly that which might be associated with local genetic adaptation and dietary specialization of sage-grouse. We assembled a reference genome and performed whole-genome sequencing across sage-grouse from six populations, encompassing both species and including several populations on the periphery of the species ranges. Population genomic analyses reaffirmed genome-wide differentiation between greater and Gunnison sage-grouse, revealed pronounced intraspecific population structure, and highlighted important differentiation of a small isolated population of greater sage-grouse in the northwest of the range. Patterns of genome-wide differentiation were largely consistent with a hypothesized role of genetic drift due to limited gene flow among populations. Inferred ancient population demography suggested persistent declines in effective population sizes that have likely contributed to differentiation within and among species. Several genomic regions with single-nucleotide polymorphisms exhibiting extreme population differentiation were associated with candidate genes linked to metabolism of xenobiotic compounds. In vitro activity of enzymes isolated from sage-grouse livers supported a role for these genes in detoxification of sagebrush, suggesting that the observed interpopulation variation may underlie important local dietary adaptations, warranting close consideration for conservation strategies that link sage-grouse to the chemistry of local sagebrush.


Assuntos
Artemisia/metabolismo , Genômica/métodos , Animais , Artemisia/genética , Sistema Enzimático do Citocromo P-450/genética , Sistema Enzimático do Citocromo P-450/metabolismo , Ecossistema
19.
Mater Sci Eng C Mater Biol Appl ; 100: 82-93, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30948119

RESUMO

An improved active packaging system was developed for fresh fruits using silver nanoparticles (AgNPs) coupled with calcium alginate (Ca-ALG). For the synthesis of AgNPs aqueous, ethanol and methanol extracts of Artemisia scoparia (AS) were used. These AgNP's were characterized using UV-Vis, SEM, EDS, AFM, FTIR and gel electrophoresis. Ethanol extract of AS (ASE) produced AgNPs with smallest size in comparison to aqueous AS (ASA) and methanol extract of AS (ASM). AgNPs synthesized from ASE had a size range of 12.0-23.3 nm and were tested on Human Corneal Epithelial Cells to evaluate their cytotoxicity. At 0.05 ng/mL of AgNP's concentration, no toxic effects were observed on the evaluated cell line. Therefore, 0.05 ng/mL of AgNPs mixed with edible coating of Ca-ALG were applied on strawberries and loquats as active coating to increase their shelf life. Significant improvement was observed in the quality parameters of strawberries and loquats such as microbial analysis, acidity loss, soluble solid content loss, weight loss and quality decay. Ca-ALG coating incorporated with AgNPs enhanced the shelf life of strawberries and loquats in comparison to no treatment and simple Ca-ALG coatings. This study provides an insight to food industry to extend the shelf life of fresh fruits using AgNP's formulated coatings.


Assuntos
Artemisia/química , Embalagem de Alimentos/métodos , Nanopartículas Metálicas/química , Prata/química , Artemisia/metabolismo , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Frutas/química , Frutas/microbiologia , Humanos , Concentração de Íons de Hidrogênio , Nanopartículas Metálicas/toxicidade , Extratos Vegetais/química
20.
Artif Cells Nanomed Biotechnol ; 47(1): 1122-1131, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30942629

RESUMO

Clinically, available synthetic chemotherapeutics in the treatment for leishmaniasis are associated with serious complications, such as toxicity and emergence of resistance. Natural products from plants can provide better remedies against the Leishmania parasite and can possibly minimize the associated side effects. In this study, various extracts of the callus cultures of Artimisia scoparia established in response to different plant growth regulators (PGRs) were evaluated for their anti-leishmanial effects against Leishmania tropica promastigotes, followed by an investigation of the possible mechanism of action through reactive apoptosis assay using fluorescent microscopy. Amongst the different callus extracts, higher anti-leishmanial activity (IC50:19.13 µg/mL) was observed in the callus raised in-vitro in the presence of 6-Benzylaminopurine (BA) plus 2,4-Dichlorophenoxyacetic Acid (2,4-D) at the concentration of 1.5 mg/L, each. Further, the results of apoptosis assay showed a large number of early-stage apoptotic (EA) and late-stage apoptotic (LA) cells in the Leishmania under the effect of callus extract grown in-vitro at BA plus 2,4-D. For the determination of the potent natural products in the callus extracts responsible for the anti-leishmanial activity, extracts were subjected to Gas chromatography-mass spectrometry (GC-MS) for the metabolite analysis. Nonetheless, higher levels of the metabolites, such as nerolidol (22%), pelletierine (18%), aspidin (15%) and ascaridole (11%) were detected in the callus grown in vitro at BA plus 2,4-D (1.5 mg/L, each). This protocol determines a novel method of production of anti-leishmanial natural products through callus cultures of A. scoparia, a medicinal plant.


Assuntos
Artemisia/crescimento & desenvolvimento , Artemisia/metabolismo , Produtos Biológicos/metabolismo , Produtos Biológicos/farmacologia , Leishmania tropica/efeitos dos fármacos , Extratos Vegetais/biossíntese , Extratos Vegetais/farmacologia , Antioxidantes/química , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Antiprotozoários/química , Antiprotozoários/metabolismo , Antiprotozoários/farmacologia , Apoptose/efeitos dos fármacos , Produtos Biológicos/química , Linhagem Celular , Flavonoides/análise , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/metabolismo , Sequestradores de Radicais Livres/farmacologia , Leishmania tropica/citologia , Extratos Vegetais/química , Polifenóis/análise
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...