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1.
Dev Comp Immunol ; 99: 103405, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31145913

RESUMO

The Gamma interferon inducible lysosomal thiol reductase (GILT) plays a key biological role in the immune responses and involves in the processing of class II MHC-restricted antigen by stimulating disulfide bond reduction in mammals. To determine the biological function of GILT in the innate immune system of crustaceans, we sequenced and cloned GILT gene from red swamp crayfish, Procambarus clarkii (Pc-GILT). The deduced amino acid sequence of Pc-GILT contained the putative conserved structures of the GILT family proteins: the GILT signature (CQHGX2ECX2NX4C) sequence and the active site (CXXS) motif. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis suggested that a recombinant Pc-GILT protein was successfully expressed in Escherichia coli (E. coli). Quantitative real-time PCR analysis showed that Pc-GILT transcript level was highest in the hepatopancreas followed by the gut, heart and muscles. Additionally, we analyzed the transcription level of Pc-GILT gene in hepatopancreas of red swamp crayfish under biotic stress conditions. The expression of Pc-GILT gene upregulated after viral (poly I:C) and bacterial (peptidoglycan, lipopolysaccharide) infection. The suppression of Pc-GILT by double stranded RNA influenced the transcript levels of various immune-related genes. These observations indicate that the Pc-GILT probably plays a key biological role in the innate immune responses of red swamp crayfish, since it modulates the expression of genes associated with immune pathways.


Assuntos
Proteínas de Artrópodes/imunologia , Astacoidea/imunologia , Imunidade Inata/genética , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/imunologia , Sequência de Aminoácidos , Animais , Proteínas de Artrópodes/antagonistas & inibidores , Proteínas de Artrópodes/química , Proteínas de Artrópodes/genética , Astacoidea/classificação , Astacoidea/genética , Sequência de Bases , Expressão Gênica , Regulação da Expressão Gênica/imunologia , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/antagonistas & inibidores , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/química , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/genética , Filogenia , Interferência de RNA , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Alinhamento de Sequência , Estresse Fisiológico/genética , Estresse Fisiológico/imunologia , Distribuição Tecidual
2.
Sci Rep ; 8(1): 2430, 2018 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-29403068

RESUMO

The pancrustacean theory groups crustaceans and hexapods (once thought to comprise separate clades within the Arthropoda) into a single clade. A key feature common to all pancrustaceans is their chitinous exoskeleton, with a major contribution by cuticular proteins. Among these, are the CPAP3's, a family of cuticular proteins, first identified in the hexapod Drosophila melanogaster and characterized by an N-terminal signaling peptide and three chitin-binding domains. In this study, CPAP3 proteins were mined from a transcriptomic library of a decapod crustacean, the crayfish Cherax quadricarinatus. Phylogenetic analysis of other CPAP3 proteins from hexapods and other crustaceans showed a high degree of conservation. Characterization of the crayfish proteins, designated CqCPAP3's, suggested a major role for CPAP3'sin cuticle formation. Loss-of-function experiments using RNAi supported such a notion by demonstrating crucial roles for several CqCPAP3 proteins during molting. A putative mode of action for the CqCPAP3 proteins -theoretically binding three chitin strands- was suggested by the structural data obtained from a representative recombinant CqCPAP3. The similarities between the CqCPAP3 proteins and their hexapod homologues further demonstrated common genetic and proteinaceous features of cuticle formation in pancrustaceans, thereby reinforcing the linkage between these two highly important phylogenetic groups.


Assuntos
Proteínas de Artrópodes/química , Astacoidea/genética , Quitina/química , Insetos/genética , Filogenia , Transcriptoma , Exoesqueleto/química , Exoesqueleto/metabolismo , Animais , Proteínas de Artrópodes/antagonistas & inibidores , Proteínas de Artrópodes/genética , Proteínas de Artrópodes/metabolismo , Astacoidea/classificação , Astacoidea/metabolismo , Biomineralização/genética , Quitina/biossíntese , Quitina/genética , Clonagem Molecular , Escherichia coli/genética , Escherichia coli/metabolismo , Expressão Gênica , Vetores Genéticos/química , Vetores Genéticos/metabolismo , Insetos/classificação , Insetos/metabolismo , Muda , Isoformas de Proteínas/antagonistas & inibidores , Isoformas de Proteínas/química , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo
3.
J Biosci ; 43(1): 189-223, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29485126

RESUMO

In the last 15 years, considerable attempts have been undertaken to develop the obligately parthenogenetic marbled crayfish Procambarus virginalis as a new model in biology. Its main advantage is the production of large numbers of offspring that are genetically identical to the mother, making this crustacean particularly suitable for research in epigenetics. Now, a draft genome, transcriptome and genome-wide methylome are available opening new windows for research. In this article, I summarize the biological advantages and genomic and epigenetic features of marbled crayfish and, based on first promising data, discuss what this new model could contribute to answering of ''big'' biological questions. Genome mining is expected to reveal new insights into the genetic specificities of decapod crustaceans, the genetic basis of arthropod reproduction, moulting and immunity, and more general topics such as the genetic underpinning of adaptation to fresh water, omnivory, biomineralization, sexual system change, behavioural variation, clonal genome evolution, and resistance to cancer. Epigenetic investigations with the marbled crayfish can help clarifying the role of epigenetic mechanisms in gene regulation, tissue specification, adult stem cell regulation, cell ageing, organ regeneration and disease susceptibility. Marbled crayfish is further suitable to elucidate the relationship between genetic and epigenetic variation, the transgenerational inheritance of epigenetic signatures and the contribution of epigenetic phenotype variation to the establishment of social hierarchies, environmental adaptation and speciation. These issues can be tackled by experiments with highly standardized laboratory lineages, comparison of differently adapted wild populations and the generation of genetically and epigenetically edited strains.


Assuntos
Proteínas de Artrópodes/genética , Astacoidea/genética , Epigênese Genética , Genoma , Partenogênese , Adaptação Fisiológica , Animais , Proteínas de Artrópodes/metabolismo , Astacoidea/classificação , Astacoidea/crescimento & desenvolvimento , Astacoidea/metabolismo , Comportamento Animal , Metilação de DNA , Feminino , Regulação da Expressão Gênica , Engenharia Genética/métodos , Especiação Genética , Masculino , Processos de Determinação Sexual , Transcriptoma
4.
Mol Phylogenet Evol ; 118: 88-98, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-28966124

RESUMO

To further understand the evolutionary history and mitogenomic features of Australia's highly distinctive freshwater crayfish fauna, we utilized a recently described rapid mitogenome sequencing pipeline to generate 24 new crayfish mitogenomes including a diversity of burrowing crayfish species and the first for Astacopsis gouldi, the world's largest freshwater invertebrate. Whole mitogenome-based phylogeny estimates using both Bayesian and Maximum Likelihood methods substantially strengthen existing hypotheses for systematic relationships among Australian freshwater crayfish with evidence of pervasive diversifying selection and accelerated mitochondrial substitution rate among the members of the clade representing strongly burrowing crayfish that may reflect selection pressures for increased energy requirement for adaptation to terrestrial environment and a burrowing lifestyle. Further, gene rearrangements are prevalent in the burrowing crayfish mitogenomes involving both tRNA and protein coding genes. In addition, duplicated control regions were observed in two closely related Engaeus species, together with evidence for concerted evolution. This study significantly adds to the understanding of Australian freshwater crayfish evolutionary relationships and suggests a link between mitogenome evolution and adaptation to terrestrial environments and a burrowing lifestyle in freshwater crayfish.


Assuntos
Astacoidea/classificação , DNA Mitocondrial/genética , Evolução Molecular , Animais , Astacoidea/genética , Austrália , Teorema de Bayes , Códon , DNA/química , DNA/isolamento & purificação , DNA/metabolismo , DNA Mitocondrial/química , DNA Mitocondrial/classificação , DNA Mitocondrial/metabolismo , Água Doce , Ordem dos Genes , Funções Verossimilhança , Filogenia , Análise de Sequência de DNA
5.
Sci Rep ; 7(1): 8272, 2017 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-28811671

RESUMO

Discerning the dispersal patterns of invasive species is critically important for the design of effective management strategies and the development of appropriate theoretical models predicting the spatial expansion of introduced populations. Post-introduction dispersal may occur naturally or via human transport, but for many organisms, assessing the relative contribution of each of these factors is difficult using traditional methods. Here, we explored the genetic patterns associated with the spread of red swamp crayfish (Procambarus clarkii) among 21 populations in the Pearl River basin and 2 peripheral populations in the Yangtze River basin. We found the genetic diversity of P. clarkii in the Pearl River basin was somewhat lower than in the Yangtze River basin. We also found (1) there was significant genetic differentiation between populations, (2) genetic differentiation was not related to geographic distance (i.e., isolation by distance), and (3) a Bayesian assignment analysis revealed three distinct genetic clusters and genetic admixture. Our results therefore provide evidence that human-mediated multiple introductions occurred in the Pearl River basin. Anthropogenic activities such as commercial transportation were likely responsible for the long-distance dispersal of P. clarkii. This study provides useful information for developing management strategies.


Assuntos
Astacoidea/genética , Repetições de Microssatélites , Rios , Agricultura , Animais , Astacoidea/classificação , China , Evolução Molecular , Variação Genética , Genótipo
6.
Evolution ; 71(10): 2522-2532, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28804900

RESUMO

Caves are perceived as isolated, extreme habitats with a uniquely specialized biota, which long ago led to the idea that caves are "evolutionary dead-ends." This implies that cave-adapted taxa may be doomed for extinction before they can diversify or transition to a more stable state. However, this hypothesis has not been explicitly tested in a phylogenetic framework with multiple independently evolved cave-dwelling groups. Here, we use the freshwater crayfish, a group with dozens of cave-dwelling species in multiple lineages, as a system to test this hypothesis. We consider historical patterns of lineage diversification and habitat transition as well as current patterns of geographic range size. We find that while cave-dwelling lineages have small relative range sizes and rarely transition back to the surface, they exhibit remarkably similar diversification patterns to those of other habitat types and appear to be able to maintain a diversity of lineages through time. This suggests that cave adaptation is not a "dead-end" for freshwater crayfish, which has positive implications for our understanding of biodiversity and conservation in cave habitats.


Assuntos
Adaptação Fisiológica , Astacoidea/genética , Evolução Molecular , Filogenia , Animais , Astacoidea/classificação , Cavernas
7.
BMC Evol Biol ; 17(1): 122, 2017 05 30.
Artigo em Inglês | MEDLINE | ID: mdl-28558646

RESUMO

BACKGROUND: The noble crayfish (Astacus astacus) displays a complex historical and contemporary genetic status in Europe. The species divergence has been shaped by geological events (i.e. Pleistocene glaciations) and humanly induced impacts (i.e. translocations, pollution, etc.) on its populations due to species commercial value and its niche degradation. Until now, limited genetic information has been procured for the Balkan area and especially for the southernmost distribution of this species (i.e. Greece). It is well known that the rich habitat diversity of the Balkan Peninsula offers suitable conditions for genetically diversified populations. Thus, the present manuscript revisits the phylogenetic relationships of the noble crayfish in Europe and identifies the genetic make-up and the biogeographical patterns of the species in its southern range limit. RESULTS: Mitochondrial markers (i.e. COI and 16S) were used in order to elucidate the genetic structure and diversity of the noble crayfish in Europe. Two of the six European haplotypic lineages, were found exclusively in Greece. These two lineages exhibited greater haplotypic richness when compared with the rest four (of "Central European" origin) while they showed high genetic diversity. Divergence time analysis identified that the majority of this divergence was captured through Pleistocene, suggesting a southern glacial refugium (Greece, southern Balkans). Furthermore, six microsatellite markers were used in order to define the factors affecting the genetic structure and demographic history of the species in Greece. The population structure analysis revealed six to nine genetic clusters and eight putative genetic barriers. Evidence of bottleneck effects in the last ~5000 years (due to climatic and geological events and human activities) is also afforded. Findings from several other research fields (e.g. life sciences, geology or even archaeology) have been utilized to perceive the genetic make-up of the noble crayfish. CONCLUSIONS: The southernmost part of Balkans has played a major role as a glacial refugium for A. astacus. Such refugia have served as centres of expansion to northern regions. Recent history of the noble crayfish in southern Balkans reveals the influence of environmental (climate, geology and/or topology) and anthropogenic factors.


Assuntos
Astacoidea/classificação , Astacoidea/genética , Animais , Península Balcânica , DNA Mitocondrial/genética , Variação Genética , Genética Populacional , Grécia , Repetições de Microssatélites , Filogenia
8.
Sci Rep ; 6: 38658, 2016 12 07.
Artigo em Inglês | MEDLINE | ID: mdl-27924858

RESUMO

The Australian redclaw crayfish (Cherax quadricarinatus) has recently received attention as an emerging candidate for sustainable aquaculture production in Australia and worldwide. More importantly, C. quadricarinatus serves as a good model organism for the commercially important group of decapod crustaceans as it is distributed worldwide, easy to maintain in the laboratory and its reproductive cycle has been well documented. In order to better understand the key reproduction and development regulating mechanisms in decapod crustaceans, the molecular toolkit available for model organisms such as C. quadricarinatus must be expanded. However, there has been no study undertaken to establish the C. quadricarinatus neuropeptidome. Here we report a comprehensive study of the neuropeptide genes expressed in the eyestalk in the Australian crayfish C. quadricarinatus. We characterised 53 putative neuropeptide-encoding transcripts based on key features of neuropeptides as characterised in other species. Of those, 14 neuropeptides implicated in reproduction regulation were chosen for assessment of their tissue distribution using RT-PCR. Further insights are discussed in relation to current knowledge of neuropeptides in other species and potential follow up studies. Overall, the resulting data lays the foundation for future gene-based neuroendocrinology studies in C. quadricarinatus.


Assuntos
Astacoidea/genética , Gânglios/metabolismo , Neuropeptídeos/genética , Transcriptoma , Sequência de Aminoácidos , Animais , Astacoidea/classificação , Austrália , Biologia Computacional/métodos , Perfilação da Expressão Gênica , Sequenciamento de Nucleotídeos em Larga Escala , Especificidade de Órgãos/genética , Filogenia
9.
Zootaxa ; 4208(2): zootaxa.4208.2.4, 2016 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-27988532

RESUMO

The crayfish subgenus Procericambarus (genus Orconectes) found in the Eastern Highlands of the United States represents a rich assemblage of stream-dwelling macroinvertebrates. While the taxonomic status and distribution of its members were clarified in a previous study, newly diagnosed characters and newly collected specimens warranted a revision to previous hypotheses of Procericambarus membership in the southeastern United States. Using morphological characters, we describe herein a new species of crayfish occurring in the Tennessee River drainage of northern Alabama and southwestern Tennessee. We also provide a revised key for the identification of members of O. juvenilis Species Complex.


Assuntos
Astacoidea/anatomia & histologia , Astacoidea/classificação , Distribuição Animal , Animais , Astacoidea/fisiologia , Feminino , Masculino , Rios , Especificidade da Espécie
10.
Zootaxa ; 4193(2): zootaxa.4193.2.8, 2016 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-27988721

RESUMO

Cambarus (Depressicambarus) clairitae, new species, is an epigean crayfish from two drainages of the Locust Fork system in Blount and Jefferson counties, Alabama. It belongs to the halli Group in the subgenus Depressicambarus. The new species is morphologically most similar to Cambarus (Depressicambarus) englishi. They differ in a several morphological characters. Cambarus englishi has a more strongly recurved central projection, a wider areola, and a more distinct and set off rostral acumen than the new species. It also has light gray to white antennae while the antennae of the new species are brown. In addition to the description of the new species, the halli Group in Alabama is discussed.


Assuntos
Astacoidea/classificação , Alabama , Distribuição Animal , Estruturas Animais/anatomia & histologia , Estruturas Animais/crescimento & desenvolvimento , Animais , Astacoidea/anatomia & histologia , Astacoidea/crescimento & desenvolvimento , Tamanho Corporal , Ecossistema , Feminino , Masculino , Tamanho do Órgão
11.
Zootaxa ; 4158(3): 301-24, 2016 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-27615888

RESUMO

Two new species of Parastacus Huxley, 1879 are described from material collected in the state of Rio Grande do Sul, southern Brazil: Parastacus fluviatilis sp. nov. from highland streams and Parastacus caeruleodactylus sp. nov. from wetlands. Parastacus fluviatilis sp. nov. is distinguished mainly by large chelipeds with dense setae cover on the cutting edge of fingers, telson subtriangular with two lateral blunt spines and strongly concave ventral surface of lateral process of thoracic sternites 6 and 7. Parastacus caeruleodactylus sp. nov. is distinguished mainly by blue cheliped fingers and a large gap between them, reduced abdomen, dorsal and ventral margins of dactylus, propodus and carpus of second pair of pereiopods with tufts of long setae and mid-dorsal carina of exopod of uropods unarmed. According to IUCN Red List criteria both species are considered endangered. Habitat characterization and a method for defining the shape of second abdominal pleura are also provided.


Assuntos
Astacoidea/anatomia & histologia , Astacoidea/classificação , Distribuição Animal , Estruturas Animais/anatomia & histologia , Estruturas Animais/crescimento & desenvolvimento , Animais , Astacoidea/crescimento & desenvolvimento , Tamanho Corporal , Brasil , Ecossistema , Feminino , Água Doce/análise , Masculino , Tamanho do Órgão
12.
Zootaxa ; 4162(1): 173-87, 2016 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-27615965

RESUMO

A new species of burrowing crayfish, Cambarus (Jugicambarus) adustus, is described from Lewis County in northeastern Kentucky, USA. The new species is most similar morphologically to C. dubius. Cambarus adustus coloration differs from C. dubius by lacking red, orange and blue hues, and instead is brown over the entire body surface. Morphological differences between C. dubius and C. adustus exist in the form I male gonopod, with C. adustus possessing a caudal knob, while C. dubius does not. In addition, the lateral carapace of C. adustus is distinctly tuberculate, whereas in C. dubius the carapace lacks extensive tuberculation. Cambarus (J.) adustus appears to have an extremely small geographic range (~19.5 km2), and as such we suggest its consideration for both state and federal levels of protection.


Assuntos
Astacoidea/anatomia & histologia , Astacoidea/classificação , Distribuição Animal/fisiologia , Animais , Astacoidea/genética , Astacoidea/fisiologia , Complexo IV da Cadeia de Transporte de Elétrons/genética , Feminino , Kentucky , Masculino , Filogenia , Especificidade da Espécie
13.
Fish Shellfish Immunol ; 58: 59-66, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27623341

RESUMO

Drosophila Toll and mammalian Toll-like receptors (TLRs) are a family of evolutionarily conserved immune receptors that play a crucial role in the first-line defense against intruded pathogens. Activating transcription factor 4 (ATF4), a member of the ATF/CREB transcription factor family, is an important factor that participates in TLR signaling and other physiological processes. However, in crustaceans, whether ATF4 homologs were involved in TLR signaling remains unclear. In the current study, we identified a Toll homolog PcToll2 and a novel ATF4 homolog PcATF4 from Procambarus clarkii, and analyzed the likely regulatory activity of PcATF4 in PcToll2 signaling. The complete cDNA sequence of PcToll2 was 4175 bp long containing an open reading frame of 2820 bp encoding a 939-amino acid protein, and the cDNA sequence of PcATF4 was 2027 bp long with an open reading frame of 1296 bp encoding a 431-amino acid protein. PcToll2 and human TLR4 shared the high identity and they were grouped into a cluster. Furthermore, PcToll2 had a close relationship with other shrimp TLRs that possessed potential antibacterial activity. PcToll2 was highly expressed in the hemocytes, heart and gills, while PcATF4 mainly distributed in gills. Upon challenge with Vibrio parahemolyticus, PcToll2 and PcATF4 together with the antimicrobial peptides of ALF1 and ALF2 were significantly up-regulated in the hemocytes, and the PcATF4 was translocated into the nucleus. After PcToll2 silencing and challenge with Vibrio, the translocation of PcATF4 into the nucleus was inhibited and the expression of ALF1 and ALF2 was reduced, but the expression of PcDorsal and PcSTAT was not affected. Furthermore, after PcATF4 knockdown and challenge with or without Vibrio, the expression of ALF1 and ALF2 was also decreased while the expression of PcToll2 was upregulated. These results suggested that PcToll2 might regulate the expression of ALF1 and ALF2 by promoting the import of PcATF4, instead of the routine transcription factor PcDorsal, into the nucleus participating in the immune defense against Gram-negative bacteria.


Assuntos
Peptídeos Catiônicos Antimicrobianos/genética , Proteínas de Artrópodes/metabolismo , Astacoidea/imunologia , Astacoidea/microbiologia , Receptores Toll-Like/metabolismo , Vibrio parahaemolyticus/fisiologia , Fator 4 Ativador da Transcrição/química , Fator 4 Ativador da Transcrição/genética , Fator 4 Ativador da Transcrição/metabolismo , Animais , Proteínas de Artrópodes/química , Proteínas de Artrópodes/genética , Proteínas de Artrópodes/imunologia , Astacoidea/classificação , Astacoidea/genética , DNA Complementar/genética , DNA Complementar/metabolismo , Regulação da Expressão Gênica , Filogenia , Transporte Proteico/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Alinhamento de Sequência , Análise de Sequência de DNA , Análise de Sequência de Proteína , Distribuição Tecidual , Receptores Toll-Like/química , Receptores Toll-Like/genética
14.
Mol Phylogenet Evol ; 103: 26-40, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27404041

RESUMO

In this study, the evolutionary history of the white-clawed crayfish (WCC) was evaluated using large-scale datasets comprising >1350 specimens from the entire distribution range. Using species delimitation methods on mitochondrial DNA (mtDNA) sequences, we propose four primary species hypotheses for WCC. Sequences for several nuclear regions were screened but none showed significant variation within WCC. This result favours a single secondary species hypothesis and indicates the existence of a mito-nuclear discordance in WCC. Therefore, mtDNA groups were considered only as genetic units that carry information about ancient divergences within WCC and not as taxonomic units. The reconstruction of ancestral ranges and divergence time estimates were used to link the current genetic structure with paleogeographic processes. These results showed that the emergence of mtDNA groups in WCC could be related to the Messinian Salinity Crisis, the climate cooling during the Pliocene and Pleistocene, and (paleo)shifting of the Adriatic Sea coastline in the Padanovenezian Plain. The most recent common ancestor of the mtDNA groups most likely originated from Dalmatia (eastern Adriatic coast) as indicated by the reconstruction of ancestral ranges. This ecoregion, along with the Gulf of Venice Drainages, harbours a high genetic diversity and should be emphasised as an area of the highest conservation priority.


Assuntos
Astacoidea/classificação , Animais , Astacoidea/genética , DNA/química , DNA/isolamento & purificação , DNA/metabolismo , DNA Mitocondrial/classificação , DNA Mitocondrial/genética , Variação Genética , Filogenia , Filogeografia , RNA Ribossômico 16S/classificação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
15.
Fish Shellfish Immunol ; 56: 162-168, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27417230

RESUMO

Cathepsin B is a lysosomal cysteine protease of the papain-like enzyme family with multiple biological functions. In the present study, a cathepsin B gene (named PcCTSB) was cloned and characterized from the red crayfish, Procambarus clarkii. The cDNA fragments of PcCTSB was 990 bp in length. It encoded a putative protein of 329 amino acid residues with predicted molecular weight of 36.4 kDa and isoelectric point of 7.020. Sequence alignment revealed that PcCTSB protein is 53.6%-80.4% identical with those from other 10 species. The predicted tertiary structure of PcCTSB protein was highly similar to that of animals. The results of the phylogenetic analysis indicated that the PcCTSB protein could be clustered with the Eriocheir sinensis cathepsin B protein. The recombinant protein of PcCTSB was expressed successfully in Escherichia coli cells. The mRNA expressions of PcCTSB were detected in all tested tissues, particularly high in the hepatopancreas. After lipopolysaccharide (LPS) challenge, the expression levels of PcCTSB were up-regulated significantly at different time points compared with control. Our results suggested that the PcCTSB might play an important role in defending against the pathogenes infection.


Assuntos
Proteínas de Artrópodes/genética , Astacoidea/genética , Astacoidea/imunologia , Catepsina B/genética , Regulação da Expressão Gênica/imunologia , Sequência de Aminoácidos , Animais , Proteínas de Artrópodes/química , Proteínas de Artrópodes/metabolismo , Astacoidea/classificação , Astacoidea/metabolismo , Sequência de Bases , Catepsina B/química , Catepsina B/metabolismo , Clonagem Molecular , DNA Complementar/genética , DNA Complementar/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Lipopolissacarídeos/farmacologia , Filogenia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência
16.
Zootaxa ; 4144(4): 575-83, 2016 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-27470874

RESUMO

A new primary burrowing crayfish, Fallicambarus schusteri, is described from the Red River drainage of extreme southeastern Oklahoma and southwestern Arkansas and is placed in the subgenus Fallicambarus. The species occurs in roadside ditches that seasonally flood and have silt and silt-loam dominated soils. Falllicambarus schusteri differs from all other members of the genus Fallicambarus in possessing a thin gradually tapering central projection and a wide triangular cephalic process on the first pleopod of form I males, a sufflamen on the cheliped, and an antennal scale that is widest at its midpoint.


Assuntos
Astacoidea/classificação , Distribuição Animal/fisiologia , Animais , Arkansas , Astacoidea/anatomia & histologia , Astacoidea/fisiologia , Comportamento Animal , Feminino , Masculino , Oklahoma , Rios , Especificidade da Espécie
17.
Zootaxa ; 4103(1): 43-53, 2016 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-27394612

RESUMO

Examination of specimens of the crayfish species Cambarus (Hiaticambarus) longirostris and two recently described crayfish revealed the existence of an undescribed species from the Flint River watershed in northern Alabama and southern Tennessee. Cambarus (Hiaticambarus) lentiginosus differs from C. longirostris, C. (Hiaticambarus) andersoni, and C. (Hiaticambarus) diupalma in aspects of carapace and chela morphology and pigmentation pattern. Cambarus (H.) lentiginosus possesses a median carina on the dorsal surface of the acumen of the rostrum, a distinctly enlarged tubercle near the base of the opposable surface of the dactyl, and a speckled pigmentation pattern over a light base color; these characters are lacking in other known Hiaticambarus species. The known range of the species is restricted to the Flint River watershed. Cambarus (H.) lentiginosus is considered Endangered using American Fisheries Society conservation categorization.


Assuntos
Astacoidea/classificação , Alabama , Distribuição Animal , Estruturas Animais/anatomia & histologia , Estruturas Animais/crescimento & desenvolvimento , Animais , Astacoidea/anatomia & histologia , Astacoidea/crescimento & desenvolvimento , Tamanho Corporal , Ecossistema , Feminino , Masculino , Tamanho do Órgão , Rios , Tennessee
18.
Integr Zool ; 11(6): 457-468, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27264730

RESUMO

The phylogenetic relationships among imported ornamental crayfish belonging to the genus Cherax were inferred from a combined dataset of 3 mitochondrial genes (COI, 16S and 12S) and by comparison with available GenBank sequences of 14 Cherax species. Furthermore, the concordance of previously described species obtained from a wholesaler (Cherax boesemani, C. holthuisi and C. peknyi) with available GenBank sequences was verified based on COI with special respect to comparison with sequences assigned as Cherax species. Recently described species C. gherardiae, C. pulcher and C. subterigneus belong to the northern group of Cherax species. Comparison and analysis with other GenBank COI sequences show previously unreported diversity of New Guinean species, suggesting 5 putative new species. Surprisingly, species assigned to the subgenus Astaconephrops do not form a monophyletic clade; this subgenus should be reappraised relative to the purported typical morphological characteristic of the uncalcified patch on male chelae. Increasing importation of crayfish underscores the importance of accurate species identification. Use of basic molecular methods is a necessary requisite for documenting occurrence, abundance and population trends of target species. Consequently, it helps to support eventual conservation decision-making by stakeholders.


Assuntos
Astacoidea/classificação , Astacoidea/genética , Animais , DNA Mitocondrial/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Nova Guiné , Filogenia , RNA Ribossômico/genética , RNA Ribossômico 16S/genética , Especificidade da Espécie
19.
Sci Rep ; 6: 26569, 2016 05 26.
Artigo em Inglês | MEDLINE | ID: mdl-27225308

RESUMO

Freshwater biodiversity is globally threatened by various factors while severe weather events like long-term droughts may be substantially devastating. In order to remain in contact with the water or stay in a sufficiently humid environment at drying localities, the ability to withstand desiccation by dwelling in the hyporheic zone, particularly through vertical burrowing is crucial. We assessed the ability of three European native and five non-native crayfish as models to survive and construct vertical burrows in a humid sandy-clayey substrate under a simulated one-week drought. Three native species (Astacus astacus, A. leptodactylus, and Austropotamobius torrentium) suffered extensive mortalities. Survival of non-native species was substantially higher while all specimens of Cherax destructor and Procambarus clarkii survived. The native species and Pacifastacus leniusculus exhibited no ability to construct vertical burrows. Procambarus fallax f. virginalis and P. clarkii constructed bigger and deeper burrows than C. destructor and Orconectes limosus. In the context of predicted weather fluctuations, the ability to withstand desiccation through constructing vertical burrows into the hyporheic zone under drought conditions might play a significant role in the success of particular crayfish species, as well as a wide range of further hyporheic-dwelling aquatic organisms in general.


Assuntos
Astacoidea/fisiologia , Secas , Animais , Astacoidea/classificação , Biodiversidade , Água Doce , Modelos Biológicos , Análise de Sobrevida
20.
Mitochondrial DNA A DNA Mapp Seq Anal ; 27(5): 3329-30, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-25738217

RESUMO

The Austropotamobius pallipes complete mitogenome has been recovered using Next-Gen sequencing. Our sample of A. pallipes has a mitogenome of 15,679 base pairs (68.44% A + T content) made up of 13 protein-coding genes, 2 ribosomal subunit genes, 22 transfer RNAs, and a 877 bp non-coding AT-rich region. This is the first mitogenome sequenced for a crayfish from the family Astacidae and the 4(th) for northern hemisphere genera.


Assuntos
Astacoidea/classificação , Astacoidea/genética , Genoma Mitocondrial , Animais , Composição de Bases , Códon , Biologia Computacional/métodos , Ordem dos Genes , Genes Mitocondriais , Tamanho do Genoma , Fases de Leitura Aberta , Análise de Sequência de DNA , Sequenciamento Completo do Genoma
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