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1.
Khirurgiia (Mosk) ; (10): 44-48, 2020.
Artigo em Russo | MEDLINE | ID: mdl-33047585

RESUMO

OBJECTIVE: To reveal the association of tension-free inguinal hernia repair and pathospermia in fertile men. MATERIAL AND METHODS: We have retrospectively analyzed medical records of 512 men who appealed to andrologist with complaints of the absence of pregnancy in wife in 2018. We evaluated duration and features of infertility, presence/absence of previous inguinal hernia repair, spermogram data (according to WHO criteria, 2010) in all patients. RESULTS: Duration of infertility in men after inguinal hernia repair persists for 4.2±2.1 years. Right-sided hernia repair was performed in 36 (48.6%) patients, left-side - 23 (31%), bilateral repair - 15 (20.2%) patients. Men with impaired sperm motility prevailed among patients after right-sided inguinal hernia repair (17 (47.2%) people). Left-sided hernia repair was followed by asthenozoospermia in 8 (34.7%) cases, bilateral hernia repair - in 3 (20%) cases. The most severe abnormalities in semen analysis (azoospemia) develop after bilateral hernia repair. CONCLUSION: Inguinal tension-free hernia repair is a risk factor for male infertility in 14.4% of cases. It is very important to examine a man in case of infertile marriage. Previous surgical interventions including inguinal hernia repair should be considered.


Assuntos
Hérnia Inguinal/cirurgia , Herniorrafia/efeitos adversos , Infertilidade Masculina/etiologia , Astenozoospermia/diagnóstico , Astenozoospermia/etiologia , Azoospermia/diagnóstico , Azoospermia/etiologia , Herniorrafia/métodos , Humanos , Infertilidade Masculina/diagnóstico , Masculino , Estudos Retrospectivos , Fatores de Risco , Análise do Sêmen
2.
Andrologia ; 52(1): e13379, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31746488

RESUMO

Male infertility is commonly associated with sperm abnormalities including asthenozoospermia. The molecular basis of asthenozoospermia was linked to mitochondrial DNA (mtDNA) mutations. The 4,977-bp human mtDNA deletion is one of the most common mutations of spermatozoa and results in loss of about 33% of the mitochondrial genome. In this preliminary study, we aimed to investigate the presence of 4,977-bp mtDNA deletion in asthenozoospermic infertile men in Jordan. Semen specimens of 120 asthenozoospermic infertile men and 80 normozoospermic individuals were collected at the in vitro fertilization unit. MtDNA was extracted after the enrichment of spermatozoa; then, polymerase chain reaction was performed using 4,977-bp mtDNA deletion-specific primers. The deletion of 4,977-bp mtDNA was detected in 79.2% of asthenozoospermic patients compared to 10% in normozoospermic controls. The results showed a significant association between the presence of 4,977-bp mtDNA deletion and the asthenozoospermia and infertility (OR = 34.2000, 95% CI = 14.57-80.26, p-value < .001). In conclusion, our findings underscored a strong association between 4,977-bp mtDNA deletion and asthenozoospermia in the Jordanian population.


Assuntos
Astenozoospermia/genética , DNA Mitocondrial/genética , Deleção de Sequência , Motilidade Espermática/genética , Espermatozoides/patologia , Astenozoospermia/diagnóstico , Astenozoospermia/patologia , Sequência de Bases/genética , Estudos de Casos e Controles , DNA Mitocondrial/isolamento & purificação , Humanos , Jordânia , Masculino
3.
Andrologia ; 52(1): e13415, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31670423

RESUMO

Oligoasthenozoospermia is a common factor leading to male infertility. Acupuncture has been applied for treating male infertility for several thousand years in China, but clinical evidence of its efficacy and safety in treating oligoasthenozoospermia is yet to be established. This review aimed to systematically assess the evidence on the effect of acupuncture in males with oligoasthenozoospermia. Databases (PubMed, EMBASE, SINOMED, CNKI, Wanfang database and Cochrane Library) were searched to identify related studies published before 30th June 2019. The Cochrane risk of bias tool and Jadad score was adopted to assess the methodological quality of included studies. Twelve randomised controlled trials (RCTs) with 1,088 participants were included in this review. The aim of this study was to perform a meta-analysis, but it was not possible due to considerable clinical heterogeneity among the included studies. According to the narrative analysis, acupuncture or acupuncture combined with another intervention was effective in improving the semen quality based on the included studies. However, this result should be interpreted with caution due to high risk. The methodological quality of most included studies was low. The current evidence on acupuncture for oligoasthenozoospermia is inadequate to draw a solid conclusion due to the poor methodological quality. Rigorous full-scale RCTs are needed to validate the therapeutic efficacy and safety of acupuncture in treating oligoasthenozoospermia.


Assuntos
Terapia por Acupuntura/efeitos adversos , Astenozoospermia/terapia , Oligospermia/terapia , Astenozoospermia/diagnóstico , Humanos , Masculino , Oligospermia/diagnóstico , Ensaios Clínicos Controlados Aleatórios como Assunto , Análise do Sêmen , Resultado do Tratamento
4.
Hum Reprod ; 34(10): 2071-2079, 2019 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-31621862

RESUMO

The use of high-throughput sequencing techniques has allowed the identification of numerous mutations in genes responsible for severe astheno-teratozoospermia due to multiple morphological abnormalities of the sperm flagella (MMAF). However, more than half of the analysed cases remain unresolved suggesting that many yet uncharacterised gene defects account for this phenotype. Based on whole-exome sequencing data from a large cohort of 167 MMAF-affected subjects, we identified two unrelated affected individuals carrying a homozygous deleterious mutation in CFAP70, a gene not previously linked to the MMAF phenotype. One patient had a homozygous splice variant c.1723-1G>T, altering a consensus splice acceptor site of CFAP70 exon 16, and one had a likely deleterious missense variant in exon 3 (p.Phe60Ile). The CFAP70 gene encodes a regulator protein of the outer dynein arms (ODA) strongly expressed in the human testis. In the sperm cells from the patient carrying the splice variant, immunofluorescence (IF) experiments confirmed the absence of the protein in the sperm flagellum. Moreover, IF analysis showed the absence of markers for the ODAs and the central pair complex of the axoneme. Interestingly, whereas CFAP70 staining was present in sperm cells from patients with mutations in the three other MMAF-related genes ARMC2, FSIP2 and CFAP43, we observed an absence of staining in sperm cells from patients mutated in the WDR66 gene, suggesting a possible interaction between two different axonemal components. In conclusion, this work provides the first evidence that loss of CFAP70 function causes MMAF and that ODA-related proteins may be crucial for the assembly and/or stability of the flagellum axoneme in addition to its motility.


Assuntos
Astenozoospermia/genética , Proteínas Associadas aos Microtúbulos/genética , Cauda do Espermatozoide/patologia , Astenozoospermia/diagnóstico , Astenozoospermia/patologia , Axonema/patologia , Análise Mutacional de DNA , Éxons/genética , Homozigoto , Humanos , Masculino , Proteínas Associadas aos Microtúbulos/metabolismo , Mutação , Mutação de Sentido Incorreto , Sítios de Splice de RNA/genética , Índice de Gravidade de Doença , Sequenciamento Completo do Exoma
5.
Hum Reprod ; 34(6): 1155-1164, 2019 06 04.
Artigo em Inglês | MEDLINE | ID: mdl-31119281

RESUMO

STUDY QUESTION: Can whole exome sequencing (WES) reveal a novel pathogenic variant in asthenozoospermia in a multiplex family including multiple patients? SUMMARY ANSWER: Patients were discovered to be homozygous for a rare 2-bp deletion in the ADCY10 coding region (c.1205_1206del, rs779944215). WHAT IS KNOWN ALREADY: ADCY10 encodes for soluble adenylyl cyclase (sAC), which is the predominant adenylate cyclase in sperm. It is already established that proper sAC activity and a constant supply of cAMP are crucial to sperm motility regulation, and knockout mouse models have been reported as severely asthenozoospermic. ADCY10 is a susceptibility gene for dominant absorptive hypercalciuria (OMIM#143870); however, no ADCY10 variations have been confirmed to cause human asthenozoospermia to date. STUDY DESIGN, SIZE, DURATION: This was a retrospective genetics study of a highly consanguineous pedigree of asthenozoospermia. The subject family was recruited in Iran in 2016. PARTICIPANTS/MATERIALS, SETTING, METHODS: The two patients were diagnosed as asthenozoospermic through careful clinical investigations. Both patients, respective parents, and an unaffected brother were subjected to WES. The discovered variant was validated by Sanger sequencing and segregated with the phenotype. To confirm the pathogenicity of the variant, sperm samples from both patients, 10 normozoospermic men and 10 asthenozoospermic patients not representing the variation, were treated with a cAMP analogue dissolved in human tubal fluid medium, followed by computer-assisted sperm analysis and statistical analyses. MAIN RESULTS AND THE ROLE OF CHANCE: The discovered homozygous variant occurs at 10 amino acids upstream of the ADCY10 nucleotide binding site leading to a premature termination (p.His402Argfs*41). Treatment of the patients' sperm samples with a cell-permeable cAMP analogue resulted in a significant increase in sperm motility, indicating the pathogenic role of the variant. Moreover, absorptive hypercalciuria, segregating within the family, was also associated with the same variant following a dominant inheritance. LIMITATIONS, REASONS FOR CAUTION: Though nonsense-mediated decay is highly likely to occur in the mutated transcripts, we were not able to confirm this due to low RNA levels in mature sperm. WIDER IMPLICATIONS OF THE FINDINGS: Our finding enlarges the phenotypic spectrum associated with the ADCY10 gene, previously described as a susceptibility gene for dominant absorptive hypercalciuria. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by grants from the Royan Institute, Tehran, Iran, and San Raffaele Hospital, Milan, Italy. The authors have no conflict of interest. TRIAL REGISTRATION NUMBER: N/A.


Assuntos
Adenilil Ciclases/genética , Astenozoospermia/genética , Hipercalciúria/genética , Cálculos Renais/genética , Adulto , Astenozoospermia/diagnóstico , Cálcio/urina , Consanguinidade , CMP Cíclico/análogos & derivados , CMP Cíclico/farmacologia , Análise Mutacional de DNA , Mutação da Fase de Leitura , Homozigoto , Humanos , Hipercalciúria/diagnóstico , Hipercalciúria/urina , Irã (Geográfico) , Cariotipagem , Cálculos Renais/diagnóstico , Cálculos Renais/urina , Masculino , Linhagem , Motilidade Espermática/efeitos dos fármacos , Motilidade Espermática/genética , Resultado do Tratamento
6.
Fertil Steril ; 111(5): 897-908.e2, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-31029245

RESUMO

OBJECTIVE: To characterize in depth and investigate the role of exosomes present in seminal plasma in affecting parameters underlying sperm activity. DESIGN: In vitro experimental study. SETTING: Research hospital. PATIENT(S): Normozoospermic, severe asthenozoospermic, and post-vasectomy azoospermic men 18-55 years of age were considered for the study. Seminal plasma was collected and processed to separate spermatozoa and exosomes. INTERVENTION(S): None. MAIN OUTCOMES MEASURE(S): Exosomes from seminal plasma were isolated and characterized by means of nanoparticle tracking analysis, transmission electron microscopy and Western blot. Exosome uptake by spermatozoa was monitored by means of immunofluorescence and flow cytometry. The effect of exosomes on spermatozoa was determined by evaluating progressive motility and capacitation, the latter assessed by means of tyrosine phosphorylation and acrosome reaction. RESULT(S): We isolated and characterized exosomes from seminal plasma of normo-, astheno-, and azoospermic patients. They display similar features in terms of shape, size, expression of canonic exosome markers and proteins involved in spermatozoa maturation, and fertilization capacity. After ejaculation, sperm cells are still receptive and are able to take up exosomes in a time- and pH-dependent manner. Exosomes derived from normozoospermic but not from asthenozoospermic individuals improve spermatozoa motility and trigger capacitation. Transfer of cysteine-rich secretory protein 1 from exosomes to spermatozoa may have a role in these phenomena. CONCLUSION(S): These findings provide evidence that: 1) sperm can still receive vesicle-derived cargo after ejaculation; 2) sperm motility and ability to undergo capacitation can benefit from exosomal transfer; and 3) semen quality is affected by male tract exosomes.


Assuntos
Astenozoospermia/diagnóstico , Exossomos/fisiologia , Sêmen/fisiologia , Capacitação Espermática/fisiologia , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Adolescente , Adulto , Astenozoospermia/genética , Astenozoospermia/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Adulto Jovem
7.
J Assist Reprod Genet ; 36(2): 267-275, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30397898

RESUMO

PURPOSE: Alternations to the paternal epigenome, specifically the components of sperm chromatin, can lead to infertility in humans and potentially transmit aberrant information to the embryo. One key component of sperm chromatin is the post-translational modification of histones (PTMs). We previously identified a comprehensive profile of histone PTMs in normozoospermic sperm; however, only specific histone PTMs have been identified in abnormal sperm by antibody-based approaches and comprehensive changes to histone PTM profiles remain unknown. Here, we investigate if sperm with abnormalities of total motility, progressive motility, and morphology have altered histone PTM profiles compared to normozoospermic sperm samples. METHODS: Discarded semen samples from 31 men with normal or abnormal semen parameters were analyzed for relative abundance of PTMs on histone H3 and H4 by "bottom-up" nano-liquid chromatography-tandem mass spectrometry. RESULTS: Asthenoteratozoospermic samples (abnormal motility, forward progression, and morphology, n = 6) displayed overall decreased H4 acetylation (p = 0.001) as well as alterations in H4K20 (p = 0.003) and H3K9 methylation (p < 0.04) when compared to normozoospermic samples (n = 8). Asthenozoospermic samples (abnormal motility and progression, n = 5) also demonstrated decreased H4 acetylation (p = 0.04) and altered H4K20 (p = 0.005) and H3K9 methylation (p < 0.04). Samples with isolated abnormal progression (n = 6) primarily demonstrated decreased acetylation on H4 (p < 0.02), and teratozoospermic samples (n = 6) appeared similar to normozoospermic samples (n = 8). CONCLUSION: Sperm samples with combined and isolated abnormalities of total motility, progressive motility, and morphology display distinct and altered histone PTM signatures compared to normozoospermic sperm. This provides evidence that alterations in histone PTMs may be important for normal sperm function and fertility.


Assuntos
Astenozoospermia/genética , Código das Histonas/genética , Infertilidade/genética , Espermatozoides/metabolismo , Adulto , Astenozoospermia/diagnóstico , Astenozoospermia/patologia , Cromatina/genética , Epigênese Genética , Histonas/genética , Humanos , Infertilidade/diagnóstico , Infertilidade/patologia , Masculino , Processamento de Proteína Pós-Traducional/genética , Motilidade Espermática/genética , Espermatozoides/crescimento & desenvolvimento
8.
Fertil Steril ; 110(6): 1058-1066, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30396550

RESUMO

OBJECTIVE: To investigate the potential effects of TAT-PRDX2 protein supplementation to the cryopreservation medium on post-thaw sperm quality and function. DESIGN: In vitro prospective study. SETTING: Medical university hospital. PATIENT(S): Fifty normozoospermic, 50 asthenozoospermic, and 50 oligoasthenozoospermic men undergoing semen analysis for couple infertility. INTERVENTION(S): Each semen sample was divided into three aliquots: fresh, cryopreserved control (without additive), and cryopreserved with TAT-PRDX2 protein. MAIN OUTCOME MEASURE(S): Sperm motility, viability, mitochondrial potential, and DNA damage as well as reactive oxygen species (ROS) levels and lipid peroxidation were analyzed. Acrosome reaction and zona-free hamster oocyte penetration tests were performed to assess the fertilization ability of cryopreserved spermatozoa. RESULT(S): In normozoospermic and asthenozoospermic groups, the addition of 150 µg/mL TAT-PRDX2 significantly reduced intracellular ROS and malondialdehyde levels and enhanced post-thaw sperm motility and viability when compared with the cryopreserved control of the respective groups but did not produce any significant protective effect in the oligoasthenozoospermic group. Mitochondrial potential was significantly increased, whereas DNA fragmentation was significantly decreased, after TAT-PRDX2 supplementation only in the asthenozoospermic group when compared with the cryopreserved control. Although the penetration rate and the penetration index were not markedly improved, TAT-PRDX2 supplementation obviously reduced spontaneous acrosome reaction and increased calcium ionophore-induced acrosome reaction in the normozoospermic and asthenozoospermic groups. CONCLUSION(S): TAT-PRDX2 protein effectively exerted cryoprotective effects on spermatozoa by reducing intracellular ROS level and thereby improved post-thaw sperm quality and function, especially for asthenozoospermic samples. TAT-PRDX2 protein is a promising additive for developing a new and highly efficient semen cryoprotectant.


Assuntos
Criopreservação/métodos , Produtos do Gene tat/administração & dosagem , Peroxirredoxinas/administração & dosagem , Preservação do Sêmen/métodos , Espermatozoides/metabolismo , Adulto , Animais , Astenozoospermia/diagnóstico , Astenozoospermia/metabolismo , Astenozoospermia/terapia , Cricetinae , Feminino , Humanos , Masculino , Oócitos/efeitos dos fármacos , Oócitos/metabolismo , Estudos Prospectivos , Espécies Reativas de Oxigênio/metabolismo , Análise do Sêmen/métodos , Espermatozoides/efeitos dos fármacos
9.
Andrologia ; 50(7): e13048, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29808481

RESUMO

Growth hormone (GH) and insulin-like growth factor 1 (IGF-1) have been proposed to play a pivotal role in male infertility due to their anabolic effects. The aim of this study was to investigate possible associations between seminal plasma levels of GH and IGF-1 and sperm parameters. Fifty men participated in this study. Semen analysis was performed, while cell-free seminal plasma was collected following sperm centrifugation. Seminal plasma concentrations of IGF-1 and GH were determined by enzyme-linked immunosorbent assay (ELISA). Due to the presence of asthenozoospermia in all participants who presented with abnormal sperm parameters, the participants were further subdivided into normal (group A), asthenozoospermic (group B) and asthenozoospermic plus at least one additional abnormal parameter (group C). A marginally nonsignificant statistical difference (p = 0.063) was revealed between the GH levels corresponding to the asthenozoospermic and the normal group with the latter presenting with higher GH levels. A statistically significant positive correlation (p < 0.05) was noted between levels of GH and IGF-1 in group C. The above relationship has also been observed in men with low sperm concentration, vitality, volume and abnormal morphology. These novel findings require further investigation in order for the biological significance of those associations to be clarified.


Assuntos
Astenozoospermia/diagnóstico , Hormônio do Crescimento Humano/análise , Fator de Crescimento Insulin-Like I/análise , Sêmen/química , Adulto , Biomarcadores/análise , Ensaio de Imunoadsorção Enzimática , Humanos , Masculino , Pessoa de Meia-Idade , Análise do Sêmen , Contagem de Espermatozoides
10.
Chin J Integr Med ; 24(7): 483-486, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29700764

RESUMO

Oligo-astheno-teratozoospermia (OAT) which perplexes doctors and patients due to its complicated etiology, atypical symptoms and poor clinical efficacy, is a general term for the three pathological states of abnormal semen. OAT is the main factor of male infertility. It is also a hot and difficult point in present studies. Empiric drug is the most popular treatment of this disease in the modern medicine. Chinese medicine (CM) is one of the main methods for the treatment of this disease, and it has certain clinical effect. The author believes that the use of modern medical technology to make the diagnosis as clear as possible is the key to treat OAT. The combination of syndrome and disease differentiation is the main mode in the treatment of OAT. Microscopic syndrome differentiation and macroscopic evidence embodies the basic principle of "Si Wai Chuai Nei" and broadens the perspective of CM syndrome differentiation. Classification and treatment are basic methods for the treatment of OAT. The treatment should not be limited to the Shen (Kidney), instead it should focus on the whole body condition. At different stages, the treatment should also pay attention to strengthening the Pi (Spleen), nourishing the Gan (Liver) and promoting blood circulation. Complementing Chinese and Western medicine, and highlighting the characteristics and advantages of CM treatment, have a great guiding value for the treatment of OAT.


Assuntos
Astenozoospermia/terapia , Medicina Integrativa/métodos , Medicina Tradicional Chinesa/métodos , Oligospermia/terapia , Astenozoospermia/diagnóstico , Terapia Combinada , Diagnóstico Diferencial , Humanos , Masculino , Oligospermia/diagnóstico , Análise do Sêmen , Ocidente
11.
Expert Rev Mol Diagn ; 18(4): 331-346, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29540081

RESUMO

INTRODUCTION: Male infertility affects about 7% of the general male population, and it is a multifactorial, polygenic pathological condition. Known genetic factors, accounting for about 20-25% of male factor infertility, are present in each etiological category: i) hypothalamic-pituitary axis dysfunction; ii) quantitative and qualitative alterations of spermatogenesis; iii) ductal obstruction/dysfunction. Areas covered: All routinely available genetic tests are described. Indication for testing for chromosomal anomalies and Y chromosome microdeletions is based on sperm count (severe oligozoospermia/azoospermia). Mutation screening in candidate genes is indicated in specific semen/testis phenotypes. In about 40% of infertile patients, the aetiology remains unknown ('idiopathic cases') and whole exome sequencing may reveal novel genetic causes. Expert commentary: Genetic testing is essential for its relevance in clinical decision-making. For instance, it helps to avoid unnecessary surgical or medical treatments and it may provide prediction for testicular sperm retrieval. The highest frequency of genetic anomalies is observed in severe spermatogenic impairment, which can be treated with in vitro fertilization (IVF). Given the risk of transmitting genetic disorders to the future offspring through IVF, the diagnosis of known and the discovery of novel genetic factors in idiopathic infertility is of outmost clinical importance.


Assuntos
Infertilidade Masculina/genética , Astenozoospermia/diagnóstico , Astenozoospermia/genética , Azoospermia/diagnóstico , Azoospermia/genética , Deleção Cromossômica , Cromossomos Humanos Y/genética , Testes Genéticos , Hormônio Liberador de Gonadotropina/deficiência , Hormônio Liberador de Gonadotropina/genética , Humanos , Hipogonadismo/diagnóstico , Hipogonadismo/genética , Infertilidade Masculina/diagnóstico , Síndrome de Kallmann/genética , Síndrome de Klinefelter/genética , Masculino , Doenças Urogenitais Masculinas/genética , Oligospermia/diagnóstico , Oligospermia/genética , Aberrações dos Cromossomos Sexuais , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/diagnóstico , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/genética , Maturação do Esperma/genética , Recuperação Espermática , Espermatogênese/genética , Teratozoospermia/diagnóstico , Teratozoospermia/genética , Ducto Deferente/anormalidades
12.
Adv Med Sci ; 62(1): 74-77, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28189122

RESUMO

PURPOSE: C-type natriuretic peptide (CNP) is a proinflammatory peptide. The highest concentration of CNP is found in male reproductive organs. This study aims to analyze the role of N-terminal C-type natriuretic propeptide (NT-proCNP) as a new indicator of asthenozoospermia. MATERIAL/METHODS: Semen was collected after 3-5 days of sexual abstinence from 86 men. The participants were between 25 and 38 years old, 51 of which had asthenozoospermia and 35 of which had normal sperm motility. Semen was analyzed for the concentrations of NT-proCNP, spermatozoa, percentages of live sperm, and sperm exhibiting a specific type of movement. The laboratory tests and analyses were performed using accepted methods and under appropriate conditions. RESULTS: A significant difference in the concentration and motility of spermatozoa was observed between the focus and control groups. The concentration of spermatozoa in the focus group was significantly lower than in the control group (median: 38.5 vs. 69.8mln/ml [p=0.016] respectively). The progressive motility of spermatozoa demonstrated a significantly lower performance in the focus group than in the control group (median: 10.4% vs. 45% respectively). The concentration of NT-proCNP was significantly higher in the focus group (median: 29.1 vs. 17.9pmol/l; p<0.001). The Area under the Receiver Operating Characteristic (AUROC) curve for the concentration of NT-proCNP - as an indicator of asthenozoospermia - was 0.733. Participants with a concentration of NT-proCNP higher than 28.8pmol/l had asthenozoospermia with 52.9% sensitivity and 94.3% specificity. CONCLUSIONS: NT-proCNP - an indicator of inflammatory reaction - should be evaluated as an indicator of asthenozoospermia.


Assuntos
Astenozoospermia/diagnóstico , Biomarcadores/metabolismo , Peptídeo Natriurético Tipo C/metabolismo , Sêmen/metabolismo , Adulto , Astenozoospermia/metabolismo , Estudos de Casos e Controles , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Motilidade Espermática
13.
Fertil Steril ; 106(5): 1061-1069.e3, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27424049

RESUMO

OBJECTIVE: To determine whether microRNA (miRNA) expression profile is different in extracellular microvesicles collected from seminal plasma of men with oligoasthenozoospermia, to gain further insight into molecular mechanisms underlying male infertility. DESIGN: Microarray with quantitative real-time polymerase chain reaction validation and Western blot analysis confirmation. SETTING: University research and clinical institutes. PATIENT(S): A total of 24 men, including 12 oligoasthenozoospermic subfertile men and 12 normozoospermic men. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Statistically significant altered miRNA expression profiles in oligoasthenozoospermic subfertile men compared with normozoospermic fertile men. RESULT(S): Extracellular microvesicles including exosomes were isolated from seminal plasma by ultracentrifugation. Presence of exosome-specific proteins was confirmed by Western blotting. In the extracellular microvesicles, we analyzed 1,205 miRNAs by microarray and identified 36 miRNAs with altered expression levels in oligoasthenozoospermic compared with normozoospermic fertile men. Seven miRNAs were overexpressed and 29 miRNAs were underexpressed in oligoasthenozoospermic men. Using quantitative real-time polymerase chain reaction as an independent method, we confirmed the significantly higher expression levels of miR-765 and miR-1275 and the significantly lower expression level of miR-15a in oligoasthenozoospermic subfertile men as compared with the normozoospermic men. CONCLUSION(S): We identified altered expression levels of miRNAs in extracellular microvesicles from seminal plasma as part of the molecular events in the male genital tract. These miRNAs may help to understand the molecular mechanisms underlying male infertility.


Assuntos
Astenozoospermia/genética , Micropartículas Derivadas de Células/genética , Exossomos/genética , Fertilidade/genética , MicroRNAs/genética , Oligospermia/genética , Sêmen/química , Transcriptoma , Adulto , Astenozoospermia/diagnóstico , Astenozoospermia/fisiopatologia , Western Blotting , Estudos de Casos e Controles , Perfilação da Expressão Gênica/métodos , Redes Reguladoras de Genes , Marcadores Genéticos , Predisposição Genética para Doença , Alemanha , Humanos , Masculino , Análise de Sequência com Séries de Oligonucleotídeos , Oligospermia/diagnóstico , Fenótipo , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Adulto Jovem
14.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 33(3): 320-3, 2016 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-27264812

RESUMO

OBJECTIVE: To explore the stability of seminal plasma miR-122-3p and miR-141-5p and their diagnostic value for idiopathic asthenospermia. METHODS: Seminal plasma miR-122-3p and miR-141-5p with various incubation time, freeze-thaw cycles and incubation time were analyzed. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the content of miR-122-3p and miR-141-5p, with U6 snRNA as the reference. The idiopathic asthenospermia group was further divided into grade I and II based on the progressive motility percent, and the content of miR-122-3p and miR-141-5p in the two grades were compared. RESULTS: No difference was detected after incubation at room temperature and 4 degrees Celsius. But seminal plasma miR-122-3p and miR-141-5p have decreased along with the increase in freeze-thaw cycles increases. RT-PCR assayed showed that the miR-122-3p content in the idiopathic asthenospermia group was significantly lower than the control group, while miR-141-5p was significantly higher. The difference in both miR-122-3p and miR-141-5p content was statistically significant (P < 0.05). A significant difference in miR-122-3p and miR-141-5p content between a and b groups was also detected (P < 0.05). The AUC-ROC of miR-122-3p was 0.88. At the cutoff value of 1.02, the sensitivity and specificity was 83% and 84%, respectively. At the cutoff of 2.95, the AUC-ROC of miR-141-5p was 0.88. The sensitivity and specificity was 84% and 70%, respectively. CONCLUSION: MiR-122-3p and miR-141-5p in seminal plasma are stable and have certain value for the diagnosis of idiopathic asthenospermia.


Assuntos
Astenozoospermia/diagnóstico , MicroRNAs/análise , Sêmen/química , Adulto , Astenozoospermia/genética , Humanos , Masculino , MicroRNAs/química , Pessoa de Meia-Idade , Curva ROC , Reação em Cadeia da Polimerase em Tempo Real
15.
Andrologia ; 48(1): 45-50, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25880899

RESUMO

Testicular adrenal rest tumours (TARTs) have been described in patients with congenital adrenal hyperplasia (CAH). The aim of the study was to determine the prevalence of TARTs in patients with CAH, the associated factors and their impact on gonadal function. It is a prospective study concerning six young adult men with CAH, four cases with 21-hydroxylase deficiency and two cases with 11-hydroxylase deficiency. All patients were under glucocorticoid therapy. The mean age was 25 years (range: 20-31). All patients underwent a physical examination with testicular palpation, scrotal ultrasonography, a blood sample for serum testosterone, FSH, LH, inhibin B, ∆4-androstenedione and 17-OH-progesterone measurements and a semen analysis. Ultrasound revealed TARTs in four patients; three were bilateral. The mean tumour size was 6.3 ml (range: 0.02-14.1). The tumours were palpable in two cases. 17-OH-progesterone was <10 ng/ml in all cases. Decreased testosterone level was found in one case. The semen analysis revealed azoospermia in one case and poor semen quality in four patients. TARTs were common and associated with impaired spermatogenesis.


Assuntos
Hiperplasia Suprarrenal Congênita/epidemiologia , Tumor de Resto Suprarrenal/epidemiologia , Astenozoospermia/epidemiologia , Azoospermia/epidemiologia , Neoplasias Primárias Múltiplas/epidemiologia , Oligospermia/epidemiologia , Neoplasias Testiculares/epidemiologia , 17-alfa-Hidroxiprogesterona/metabolismo , Hiperplasia Suprarrenal Congênita/metabolismo , Tumor de Resto Suprarrenal/diagnóstico , Adulto , Androstenodiona/metabolismo , Astenozoospermia/diagnóstico , Azoospermia/diagnóstico , Estudos de Coortes , Hormônio Foliculoestimulante/metabolismo , Humanos , Inibinas/metabolismo , Hormônio Luteinizante/metabolismo , Masculino , Neoplasias Primárias Múltiplas/diagnóstico , Oligospermia/diagnóstico , Prevalência , Estudos Prospectivos , Contagem de Espermatozoides , Motilidade Espermática , Neoplasias Testiculares/diagnóstico , Testosterona/metabolismo , Adulto Jovem
16.
Andrology ; 3(6): 1173-82, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26446356

RESUMO

Phosholipase A2 (PLA2 ) activity in the seminal plasma and in sperm heads is closely related to sperm motility and male fertility. Therefore, the purpose of this study was to investigate the possible involvement of different isoforms of phospholipase in asthenozoospermia. To accomplish this, cPLA2 , phospho-cPLA2 , iPLA2 , and sPLA2 were evaluated by immunofluorescence and immunoblot analyses in spermatozoa obtained from 22 normozoospermic men and 28 asthenozoospermic patients. We found significant differences in cPLA2 and its phosphorylated/activated form, iPLA2 , and sPLA2 content and distribution in normal and asthenozoospermic patients. cPLA2 was localized in heads, midpieces, and tails of all spermatozoa as constitutive enzyme, less expressed in the tail of spermatozoa with low progressive motility. While active phospho-cPLA2 distribution was homogeneous throughout the cell body of control-donor spermatozoa, lower levels were detected in the tails of asthenozoospermic patients, as opposed to its strong presence in heads. Low immunofluorescence signal for iPLA2 was found in astenozoospermic patients, whereas sPLA2 was significantly lower in the heads of asthenozoospermic patients. Spermatozoa with low progressive motility showed differences both in terms of total specific activity and of intracellular distribution. cPLA2 , iPLA2 , and sPLA2 specific activities correlated positively and in a significantly manner with sperm progressive motility both in normozoospermic men and asthenozoospermic patients. In conclusion, PLA2 s are expressed in different areas of human spermatozoa. Spermatozoa with low motility showed differences in total specific activity and enzyme distributions. We speculated that PLA2 expression and/or different distribution could be potential biomarkers of asthenozoospermia, one of the major causes of male factor infertility.


Assuntos
Astenozoospermia/enzimologia , Membrana Celular/enzimologia , Fosfolipases A2 do Grupo VI/análise , Fosfolipases A2 Secretórias/análise , Espermatozoides/enzimologia , Astenozoospermia/diagnóstico , Astenozoospermia/fisiopatologia , Biomarcadores/análise , Western Blotting , Estudos de Casos e Controles , Fertilidade , Imunofluorescência , Humanos , Masculino , Microscopia Confocal , Fosforilação , Contagem de Espermatozoides , Motilidade Espermática , Espermatozoides/patologia
17.
Fertil Steril ; 104(3): 591-601, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26143365

RESUMO

OBJECTIVE: To compare the microRNA (miRNA) expression profile in spermatozoa from three infertile populations vs. a group of fertile men. DESIGN: Evaluation of the expression level of 736 miRNAs in human spermatozoa using TaqMan quantitative reverse transcription-polymerase chain reaction. SETTING: University research facility. PATIENT(S): Semen samples with a single seminal alteration were collected from infertile individuals: asthenozoospermic (n = 10), teratozoospermic (n = 10), and oligozoospermic (n = 10). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Correlation of the expression level of each miRNA with seminal parameters, age, and chromosome instability; clustering of the individuals according to their miRNA expression profiles and influence of the seminogram, age, chromosome instability, and assisted reproductive technology outcome in the clustering; analysis of the differentially expressed miRNAs (DE-miRNAs) in each infertile population; genome annotation of these DE-miRNAs; and ontological analysis of their predicted targets. RESULT(S): The hsa-miR-34b-3p correlated with age, the hsa-miR-629-3p with sperm motility, and the hsa-miR-335-5p, hsa-miR-885-5p, and hsa-miR-152-3p with sperm concentration. The individuals clustered into two groups, and only the seminogram was differentially distributed. We identified 32 DE-miRNAs in the asthenozoospermic group, 19 in the teratozoospermic group, and 18 in the oligozoospermic group. The up-regulated miRNAs presented an enriched localization in introns, affecting relevant genes for spermatogenesis. The predicted targets of the DE-miRNAs contained critical genes associated to infertility, and their ontological analysis revealed significantly associated functions related to the seminal alterations of each group. CONCLUSION(S): Spermatozoa from patients with seminal alterations exhibit a differential miRNA profile. This provides new evidence that miRNAs have an essential role in spermatogenesis, contributing to the mechanisms involved in human fertility.


Assuntos
Fertilidade/genética , Infertilidade Masculina/diagnóstico , Infertilidade Masculina/genética , MicroRNAs/genética , Espermatozoides/química , Astenozoospermia/diagnóstico , Astenozoospermia/genética , Astenozoospermia/fisiopatologia , Azoospermia/diagnóstico , Azoospermia/genética , Azoospermia/fisiopatologia , Estudos de Casos e Controles , Instabilidade Cromossômica , Análise por Conglomerados , Perfilação da Expressão Gênica/métodos , Marcadores Genéticos , Humanos , Infertilidade Masculina/fisiopatologia , Masculino , Oligospermia/diagnóstico , Oligospermia/genética , Oligospermia/fisiopatologia , Idade Paterna , Fatores de Risco , Contagem de Espermatozoides , Motilidade Espermática , Espermatozoides/patologia
19.
World J Urol ; 33(5): 633-8, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25717016

RESUMO

OBJECTIVES: To investigate the expression level of testis-specific calcium-binding protein CBP86-IV in normal and asthenozoospermic human sperm. METHODS: The total RNA was extracted from human sperm, and target cDNA was obtained by reverse transcription-polymerase chain reaction. Then the cDNA was used for quantitative PCR analysis and cloned into the prokaryotic expression vector pET-28a, respectively. The fusion protein was induced and expressed as inclusion body which was used to produce the polyclonal antibody against TSCBP86-IV. The protein expression level of TSCBP86-IV from normal human sperm and idiopathic asthenozoospermic samples was detected by the purified antibody. RESULTS: The experimental results showed that the protein expression of TSCBP86-IV was reduced in idiopathic asthenozoospermia and consistent with the transcriptional changing tendency which was detected by quantitative PCR analysis. CONCLUSIONS: The stable and reliable change of TSCBP86-IV may be taken as a new molecular marker for clinical diagnosis of idiopathic asthenozoospermia.


Assuntos
Astenozoospermia/diagnóstico , Astenozoospermia/metabolismo , Proteínas de Ligação ao Cálcio/metabolismo , Fosfoproteínas/metabolismo , Testículo/metabolismo , Adulto , Biomarcadores/metabolismo , Proteínas de Ligação ao Cálcio/genética , Estudos de Casos e Controles , Regulação da Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Fosfoproteínas/genética , RNA Mensageiro/metabolismo , Motilidade Espermática
20.
Urol Int ; 94(1): 111-6, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-24246711

RESUMO

OBJECTIVE: To evaluate whether couples with moderate male infertility should be treated with conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). PATIENTS AND METHODS: A total of 249 couples with moderate male infertility undergoing their first IVF/ICSI cycle were enrolled in the study. The couples were divided into two groups according to the results of semen analysis: moderate oligozoospermia (O group) and moderate oligoasthenozoospermia (OA group). Sibling oocytes were randomized into groups to be inseminated either by conventional IVF or ICSI. Fertilization rate, embryo quality, implantation rate, and clinical pregnancy rate were examined. RESULTS: There was no difference in the fertilization, implantation, and pregnancy rates between conventional IVF and ICSI in either the O group or OA group (p > 0.05). Additionally, in the OA group, the good quality embryo rate was similar after IVF or ICSI (p > 0.05). However, in the O group, the good quality embryo rate was significantly higher after ICSI than after IVF (p < 0.05). CONCLUSIONS: Couples with moderate oligozoospermia or moderate oligoasthenozoospermia did not influence the major indices of IVF. Because of the uncertainties concerning the safety of ICSI, couples with moderate oligozoospermia or moderate oligoasthenozoospermia need not be subjected to this procedure.


Assuntos
Astenozoospermia/terapia , Ejaculação , Fertilização In Vitro , Oligospermia/terapia , Injeções de Esperma Intracitoplásmicas , Adulto , Astenozoospermia/diagnóstico , Astenozoospermia/fisiopatologia , China , Implantação do Embrião , Transferência Embrionária , Feminino , Fertilização In Vitro/efeitos adversos , Humanos , Masculino , Oligospermia/diagnóstico , Oligospermia/fisiopatologia , Seleção de Pacientes , Gravidez , Taxa de Gravidez , Medição de Risco , Fatores de Risco , Análise do Sêmen , Injeções de Esperma Intracitoplásmicas/efeitos adversos , Resultado do Tratamento
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