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1.
Anal Bioanal Chem ; 412(3): 611-620, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31900539

RESUMO

Infections caused by foodborne microorganisms are a great threat to the global environment and public healthcare today. Thus, rapid, portable and sensitive assays that can realize the identification of foodborne bacteria are highly desired. In this study, a smart fluorescent and colorimetric dual-readout sensing system has been established for simple and rapid E. coli determination by utilizing the Cu2+-triggered oxidation of o-phenylenediamine (OPD). Initially, Cu2+ can oxidize OPD to OPDox, resulting in an orange-yellow fluorescence and visible pale-yellow color. However, E. coli can effectively reduce Cu2+ into Cu+, inhibiting the Cu2+-triggered oxidation of OPD to OPDox. Consequently, the introduction of E. coli can turn off both the fluorescence and the UV-vis absorbance signals of the OPD-Cu2+ system, illustrating an original mechanism for fluorescent and colorimetric dual-channel detection of E. coli. Moreover, a filter paper-based visual sensor was built and coupled with OPD-Cu2+ solution under the assistance of a UV lamp. The as-prepared sensor can detect E. coli quantitatively with the help of a typical smartphone color-scanning application (APP). Thus, this study offers a valid dual-mode assay for sensitive and on-site visible detection of E. coli, guaranteeing the reliability of the results and is more attractive for practical use. Graphical Abstract Schematic illustration of the smartphone-integrated sensing system for fluorescent and colorimetric dual-channel detection of E. coli based on the Cu2+-OPD system.


Assuntos
Bactérias/isolamento & purificação , Colorimetria/métodos , Corantes Fluorescentes/química , Microbiologia de Alimentos , Papel , Smartphone , Espectrometria de Fluorescência/métodos , Integração de Sistemas , Bactérias/patogenicidade , Técnicas Biossensoriais , Reprodutibilidade dos Testes , Espectrofotometria Ultravioleta/métodos
2.
Adv Exp Med Biol ; 1131: 827-855, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31646536

RESUMO

Calcium (Ca2+) is a universal signaling ion, whose major informational role shaped the evolution of signaling pathways, enabling cellular communications and responsiveness to both the intracellular and extracellular environments. Elaborate Ca2+ regulatory networks have been well characterized in eukaryotic cells, where Ca2+ regulates a number of essential cellular processes, ranging from cell division, transport and motility, to apoptosis and pathogenesis. However, in bacteria, the knowledge on Ca2+ signaling is still fragmentary. This is complicated by the large variability of environments that bacteria inhabit with diverse levels of Ca2+. Yet another complication arises when bacterial pathogens invade a host and become exposed to different levels of Ca2+ that (1) are tightly regulated by the host, (2) control host defenses including immune responses to bacterial infections, and (3) become impaired during diseases. The invading pathogens evolved to recognize and respond to the host Ca2+, triggering the molecular mechanisms of adhesion, biofilm formation, host cellular damage, and host-defense resistance, processes enabling the development of persistent infections. In this review, we discuss: (1) Ca2+ as a determinant of a host environment for invading bacterial pathogens, (2) the role of Ca2+ in regulating main events of host colonization and bacterial virulence, and (3) the molecular mechanisms of Ca2+ signaling in bacterial pathogens.


Assuntos
Bactérias , Cálcio , Interações entre Hospedeiro e Microrganismos , Virulência , Bactérias/patogenicidade , Infecções Bacterianas/microbiologia , Infecções Bacterianas/fisiopatologia , Cálcio/metabolismo , Humanos , Virulência/fisiologia
4.
Pan Afr Med J ; 34: 24, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31762893

RESUMO

Introduction: HIV infection is characterized by changes in the composition and functions of gut microbiota. We carried out a study aiming at comparing the compositional changes of the digestive flora of HIV infected infants versus that of non infected infants in Cameroon. Methods: A case-control study was carried out during which stool sample was collected from each participant after obtaining the proxy consent. Stools were cultured using aerobic, strict anaerobic, 10% CO2 and micro-aerophilic conditions and specific culture media and bacteria were identified biochemically. Fisher's exact test was used for data analyses. Results: From the 80 infants enrolled for the study, 33 (41.3%) were HIV positive. A statistically significant difference was observed between the number of infected versus non infected infants harboring the following bacteria: Clostridium spp. (P=0.009); Enterococcus spp. (p<0.001); Klebsiella (p<0.001); Shigella (<0.001); Staphylococcus aureus (p=0.006) and Streptococcus spp. (P=0.015). Among infected infants, WHO-stage 3 and 4 infants harbored more opportunistic bacteria than stage 1 and stage 2 and Bacteriodes spp. population was depleted as the disease progresses, although not significantly. There was an imbalance in bacteria flora in HIV infected infants harboring qualitatively more bacteria including more opportunistic and pathogenic bacteria than in HIV non-infected infants. Conclusion: HIV infected infants presented a qualitatively different flora from HIV non infected infants. They habored more pathogenic bacteria Than non infected infants. Systematic stool culture could benefit for follow-up of HIV infected infants to reduce the risk of gastrointestinal disorders and thus the risk of high morbidity or high mortability.


Assuntos
Bactérias/isolamento & purificação , Sistema Digestório/microbiologia , Fezes/microbiologia , Infecções por HIV/complicações , Bactérias/patogenicidade , Camarões , Estudos de Casos e Controles , Pré-Escolar , Progressão da Doença , Feminino , Humanos , Lactente , Masculino
5.
Niger J Clin Pract ; 22(10): 1378-1387, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31607727

RESUMO

Objective: The objective was to determine the antibacterial activity of Salvadora persica extract against bacteria isolated from dental plaque of patients. Materials and Methods: Out of 40 different clinical specimens collected from patients suffering from plaque-induced gingivitis, 12 Staphylococcus aureus and 8 Streptococcus sp. isolates were recovered. The isolates were screened for their biofilm-forming capacity using tissue culture plate (TCP), tube method (TM), and congo red agar (CRA) method. Antibacterial activity of methanolic S. persica extract as well as of commercial antimicrobials against tested isolates was performed. High-performance liquid chromatography-mass spectrometry (HPLC-MS) and gas chromatography-MS (GC-MS) analysis were performed for S. persica crude extract and its volatile oil, respectively, to determine their constituents. Results: Out of 20 isolates, 80%, 85%, and 90% showed positive results using TM, CRA, and TCP, respectively. The highest antimicrobial activity of methanolic S. persica extract was observed at 200 mg/ml. HPLC-MS analysis shows many polyphenols in S. persica extract such as Chrysin-8-c-ß-D-glucopyranoside, ferulic acid, gallic acid, and stigmasterol. Chemical composition of the essential oil of S. persica was determined by GC-MS yield; a mixture of monoterpene and hydrocarbons. The major compounds were butylated hydroxytoluene followed by benzene (isothiocyanatomethyl). Conclusion: Methanolic extract of S. persica had significant antibacterial effect against S. aureus and Streptococcus sp. isolates, and it may be gave a good alternative method for controlling oral pathogen.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Placa Dentária/microbiologia , Gengivite/microbiologia , Boca/efeitos dos fármacos , Extratos Vegetais/farmacologia , Salvadoraceae/química , Staphylococcus aureus/efeitos dos fármacos , Adulto , Antibacterianos/química , Antibacterianos/isolamento & purificação , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , Bactérias/patogenicidade , Cromatografia Líquida de Alta Pressão , Cárie Dentária/microbiologia , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Gengivite/tratamento farmacológico , Humanos , Masculino , Testes de Sensibilidade Microbiana , Boca/microbiologia , Extratos Vegetais/química , Streptococcus/efeitos dos fármacos
6.
Orv Hetil ; 160(39): 1533-1541, 2019 Sep.
Artigo em Húngaro | MEDLINE | ID: mdl-31544493

RESUMO

Our health is highly determined by the diverse microbial community living within our body and upon our skin. Balance among the members of the commensal microbiota is essential for the preservation of health. New generation sequencing is a rapid, sensitive method for determining the whole microbiome without prior hypothesis and also gives information on the resistance and virulence status. Application of this method can help to identify the pathogens contributing to different diseases, and also the protective bacteria inhibiting their growth. Detecting the changes of the microbiome helps to identify new therapeutic targets and establish targeted antibiotic therapy. Broad-spectrum antibiotics also act against the beneficial members of the microbial flora, which may lead to the development of recurrent or chronic disease. Ear, nose and throat infections are the most common infective diseases in humans and the leading cause for antibiotic prescription worldwide. In recent years, many studies using molecular biology methods were performed examining the microbiome of healthy humans and in otorhinolaryngologic diseases. In the present work, the authors review the changes of the microbiological communities in the healthy state and in various pathologic states in the anatomic regions of the ear, nose and throat. Orv Hetil. 2019; 160(39): 1533-1541.


Assuntos
Bactérias/classificação , Orelha/microbiologia , Microbiota , Nariz/microbiologia , Otolaringologia , Otorrinolaringopatias/microbiologia , Faringe/microbiologia , Antibacterianos/uso terapêutico , Bactérias/genética , Bactérias/patogenicidade , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/microbiologia , Humanos , Otolaringologia/tendências , Otorrinolaringopatias/tratamento farmacológico , Otorrinolaringopatias/terapia , Análise de Sequência de DNA/métodos
7.
Nihon Saikingaku Zasshi ; 74(2): 157-165, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31474648

RESUMO

Bacteria, life living at microscale, can spread only by thermal fluctuation. However, the ability of directional movement, such as swimming by rotating flagella, gliding over surfaces via mobile cell-surface adhesins, and actin-dependent movement, could be useful for thriving through searching more favorable environments, and such motility is known to be related to pathogenicity. Among diverse migration mechanisms, perhaps flagella-dependent motility would be used by most species. The bacterial flagellum is a molecular nanomachine comprising a helical filament and a basal motor, which is fueled by an electrochemical gradient of cation across the cell membrane (ion motive force). Many species, such as Escherichia coli, possess flagella on the outside of the cell body, whereas flagella of spirochetes reside within the periplasmic space. Flagellar filaments or helical spirochete bodies rotate like a screw propeller, generating propulsive force. This review article describes the current knowledge of the structure and operation mechanism of the bacterial flagellum, and flagella-dependent motility in highly viscous environments.


Assuntos
Bactérias/patogenicidade , Fenômenos Fisiológicos Bacterianos , Movimento Celular/fisiologia , Flagelos/fisiologia , Movimento/fisiologia , Bactérias/citologia , Bactérias/genética , Bactérias/metabolismo , Membrana Celular/metabolismo
8.
Cell Host Microbe ; 26(2): 173-182, 2019 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-31415750

RESUMO

Eukaryotic small RNAs (sRNAs) are short non-coding regulatory molecules that induce RNA interference (RNAi). During microbial infection, host RNAi machinery is highly regulated and contributes to reprogramming gene expression and balancing plant immunity and growth. While most sRNAs function endogenously, some can travel across organismal boundaries between hosts and microbes and silence genes in trans in interacting organisms, a mechanism called "cross-kingdom RNAi." During the co-evolutionary arms race between fungi and plants, some fungi developed a novel virulence mechanism, sending sRNAs as effector molecules into plant cells to silence plant immunity genes, whereas plants also transport sRNAs, mainly using extracellular vesicles, into the pathogens to suppress virulence-related genes. In this Review, we highlight recent discoveries on these key roles of sRNAs and RNAi machinery. Understanding the molecular mechanisms of sRNA biogenesis, trafficking, and RNAi machinery will help us develop innovative strategies for crop protection.


Assuntos
Interações Hospedeiro-Patógeno/genética , Imunidade Vegetal/genética , Plantas/genética , RNA Interferente Pequeno/genética , Arabidopsis/genética , Arabidopsis/imunologia , Bactérias/genética , Bactérias/patogenicidade , Fungos/genética , Fungos/patogenicidade , Interações Hospedeiro-Patógeno/imunologia , Doenças das Plantas/imunologia , Imunidade Vegetal/imunologia , Plantas/imunologia , Interferência de RNA/imunologia , RNA Interferente Pequeno/imunologia , Pequeno RNA não Traduzido/imunologia , Simbiose , Virulência/genética
10.
Physiol Rev ; 99(4): 1877-2013, 2019 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-31460832

RESUMO

The importance of the gut-brain axis in maintaining homeostasis has long been appreciated. However, the past 15 yr have seen the emergence of the microbiota (the trillions of microorganisms within and on our bodies) as one of the key regulators of gut-brain function and has led to the appreciation of the importance of a distinct microbiota-gut-brain axis. This axis is gaining ever more traction in fields investigating the biological and physiological basis of psychiatric, neurodevelopmental, age-related, and neurodegenerative disorders. The microbiota and the brain communicate with each other via various routes including the immune system, tryptophan metabolism, the vagus nerve and the enteric nervous system, involving microbial metabolites such as short-chain fatty acids, branched chain amino acids, and peptidoglycans. Many factors can influence microbiota composition in early life, including infection, mode of birth delivery, use of antibiotic medications, the nature of nutritional provision, environmental stressors, and host genetics. At the other extreme of life, microbial diversity diminishes with aging. Stress, in particular, can significantly impact the microbiota-gut-brain axis at all stages of life. Much recent work has implicated the gut microbiota in many conditions including autism, anxiety, obesity, schizophrenia, Parkinson's disease, and Alzheimer's disease. Animal models have been paramount in linking the regulation of fundamental neural processes, such as neurogenesis and myelination, to microbiome activation of microglia. Moreover, translational human studies are ongoing and will greatly enhance the field. Future studies will focus on understanding the mechanisms underlying the microbiota-gut-brain axis and attempt to elucidate microbial-based intervention and therapeutic strategies for neuropsychiatric disorders.


Assuntos
Bactérias/metabolismo , Encefalopatias/microbiologia , Encéfalo/microbiologia , Microbioma Gastrointestinal , Intestinos/microbiologia , Fatores Etários , Envelhecimento , Animais , Bactérias/imunologia , Bactérias/patogenicidade , Comportamento , Encéfalo/imunologia , Encéfalo/metabolismo , Encéfalo/fisiopatologia , Encefalopatias/metabolismo , Encefalopatias/fisiopatologia , Encefalopatias/psicologia , Disbiose , Sistema Nervoso Entérico/metabolismo , Sistema Nervoso Entérico/microbiologia , Sistema Nervoso Entérico/fisiopatologia , Interações Hospedeiro-Patógeno , Humanos , Intestinos/imunologia , Neuroimunomodulação , Plasticidade Neuronal , Fatores de Risco
11.
IET Nanobiotechnol ; 13(6): 597-601, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31432792

RESUMO

Nanobiotechnology is a promising field concerned with the using of engineered nanomaterials, which leads to the improvement of new human remedial against pathogenic bacteria modalities. In this work, silver nanoparticles (AgNPs) were prepared by an easy, cheap and low-cost electro-chemical method. The AgNPs were then loaded successfully on to multi-walled carbon nanotubes (MWCNTs) using a modified chemical reaction process. The AgNPs on the MWCNTs were well spread and evenly distributed on the surfaces of the long nanotubes with well-graphitised walls as examined by high-resolution transmission electron microscopy. X-ray diffraction and transmission electron microscopy were used for sample characterisation. Good dispersion of AgNPs was obtained on the surface of MWCNTs, resulting in an efficient reactivity of the carbon nanotubes surfaces. Finally, the antibacterial activity of AgNPs/MWCNTs hybrid was evaluated against two pathogenic bacteria Pseudomonas aeruginosa and Staphylococcus aureus exhibited excellent activity.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Nanotubos de Carbono , Antibacterianos/química , Bactérias/patogenicidade , Escherichia coli , Nanopartículas Metálicas/química , Nanopartículas Metálicas/toxicidade , Testes de Sensibilidade Microbiana , Viabilidade Microbiana/efeitos dos fármacos , Nanotubos de Carbono/química , Nanotubos de Carbono/microbiologia , Nanotubos de Carbono/toxicidade , Pseudomonas aeruginosa , Prata/química , Prata/farmacologia , Staphylococcus aureus , Propriedades de Superfície
12.
Food Microbiol ; 84: 103277, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31421776

RESUMO

The purpose of this study was to evaluate the synergistic bactericidal efficacy of combining ultrasound (US) and fumaric acid (FA) treatment against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes in apple juice and to identify the synergistic bactericidal mechanisms. Additionally, the effect of combination treatment on juice quality was determined by measuring the changes in color, pH, non-enzymatic browning index, and total phenolic content. A mixed cocktail of the three pathogens was inoculated into apple juice, followed by treatment with US (40 kHz) alone, FA (0.05, 0.1, and 0.15%) alone, and a combination of US and FA for 1, 2, 3, 4, and 5 min. Combined US and 0.15% FA treatment for 5 min achieved 5.67, 6.35, and 3.47 log reductions in E. coli O157:H7, S. Typhimurium, and L. monocytogenes, respectively, with the 1.55, 2.37, and 0.57 log CFU reductions attributed to the synergistic effect. Although the pH value slightly decreased as FA increased, there were no significant (P > 0.05) differences in color values, browning indices, and phenolic content between untreated and treated samples. To identify the mechanism of this synergistic bactericidal action, membrane integrity, malfunctions in the membrane efflux pump, and intracellular enzyme activity were measured. The analyses confirmed that damage to the cell envelope (membrane integrity and efflux pump) was strongly related to the synergistic microbial inactivation. These results suggest that simultaneous application of US treatment and FA is a novel method for ensuring the microbial safety of apple juice.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos da radiação , Sucos de Frutas e Vegetais/microbiologia , Fumaratos/farmacologia , Malus/microbiologia , Viabilidade Microbiana/efeitos da radiação , Ondas Ultrassônicas , Bactérias/patogenicidade , Contagem de Colônia Microbiana , Escherichia coli O157/efeitos dos fármacos , Escherichia coli O157/efeitos da radiação , Microbiologia de Alimentos/métodos , Conservação de Alimentos/métodos , Listeria monocytogenes/efeitos dos fármacos , Listeria monocytogenes/efeitos da radiação , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/efeitos da radiação
13.
Int J Mol Sci ; 20(15)2019 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-31382674

RESUMO

Both the prevalence of antibiotic resistance and the increased biofilm-associated infections are boosting the demand for new advanced and more effective treatment for such infections. In this sense, nanotechnology offers a ground-breaking platform for addressing this challenge. This review shows the current progress in the field of antimicrobial inorganic-based nanomaterials and their activity against bacteria and bacterial biofilm. Herein, nanomaterials preventing the bacteria adhesion and nanomaterials treating the infection once formed are presented through a classification based on their functionality. To fight infection, nanoparticles with inherent antibacterial activity and nanoparticles acting as nanovehicles are described, emphasizing the design of the carrier nanosystems with properties targeting the bacteria and the biofilm.


Assuntos
Bactérias/efeitos dos fármacos , Infecções Bacterianas/terapia , Nanoestruturas/uso terapêutico , Nanotecnologia , Antibacterianos/uso terapêutico , Bactérias/patogenicidade , Aderência Bacteriana/efeitos dos fármacos , Infecções Bacterianas/microbiologia , Biofilmes/efeitos dos fármacos , Humanos
14.
Int J Mol Sci ; 20(15)2019 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-31382580

RESUMO

Present day awareness of biofilm colonization on polymeric surfaces has prompted the scientific community to develop an ever-increasing number of new materials with anti-biofilm features. However, compared to the large amount of work put into discovering potent biofilm inhibitors, only a small number of papers deal with their validation, a critical step in the translation of research into practical applications. This is due to the lack of standardized testing methods and/or of well-controlled in vivo studies that show biofilm prevention on polymeric surfaces; furthermore, there has been little correlation with the reduced incidence of material deterioration. Here an overview of the most common methods for studying biofilms and for testing the anti-biofilm properties of new surfaces is provided.


Assuntos
Bactérias/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Equipamentos e Provisões/microbiologia , Polímeros/farmacologia , Bactérias/crescimento & desenvolvimento , Bactérias/patogenicidade , Biofilmes/crescimento & desenvolvimento , Humanos , Polímeros/química , Propriedades de Superfície
16.
Comp Immunol Microbiol Infect Dis ; 65: 154-159, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31300107

RESUMO

Turtoises are a great puzzle when it comes to their bacterial flora, the composition and structure of which are still unknown in details. Its component which has been best described so far is Salmonella spp., presumably due to the threat of reptile-associated salmonellosis in humans. This investigation tried to assess and characterize intestinal bacterial flora of imported tortoises found dead during quarantine. Most of the animals carried various serovars of Salmonella showing no antimicrobial resistance. Presence of multiresistant Escherichia coli was possibly a result of industrial breeding and high usage of antimicrobials. Thirteen bacterial species or genera like Citrobacter spp., Morganella spp., Pseudomonas spp. were identified. Their commensal character is assumed, although pathogenic potential might be verified. The results indicate global tortoise trade as a source of common and exotic bacteria or antimicrobial resistance mechanisms in new geographical areas. These dangers indicate the need for a systematic survey of exotic pets and establishment of legal requirements for reptile health conditions on breeding, trade premises and in households with such pets.


Assuntos
Bactérias/isolamento & purificação , Infecções Bacterianas/veterinária , Microbiota , Animais de Estimação/microbiologia , Tartarugas/microbiologia , Animais , Bactérias/efeitos dos fármacos , Bactérias/patogenicidade , Infecções Bacterianas/mortalidade , Doenças Transmissíveis Importadas/microbiologia , Doenças Transmissíveis Importadas/transmissão , Farmacorresistência Bacteriana Múltipla , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Feminino , Microbioma Gastrointestinal , Testes de Sensibilidade Microbiana , Saúde Pública , Quarentena/veterinária , Salmonella/efeitos dos fármacos , Salmonella/isolamento & purificação , Salmonelose Animal/microbiologia , Salmonelose Animal/mortalidade , Zoonoses/microbiologia , Zoonoses/transmissão
17.
Appl Microbiol Biotechnol ; 103(18): 7481-7490, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31300853

RESUMO

Cutaneous leishmaniasis being a neglected tropical disease (NTD) faces several challenges in chemotherapy. If infected with secondary bacterial infections, the treatment regime of cutaneous ulcers in cutaneous leishmaniasis is further complicated which usually require two or more than two chemotherapeutic agents for healing. In the current study, seven curcumin-loaded self-emulsifying drug delivery system (cu-SEDDS) formulations (namely F1-F7) were prepared by mixing different excipients (oils, surfactants, and co-solvents) through stirring (vortex) and sonication. The formulations were characterized regarding their droplet size, polydispersity index (PDI), and zeta potential by zeta sizer. The cu-SEDDS formulations displayed different sizes ranging from 32.4 up to 80.0 nm. The zeta potential of the formulations ranged from - 1.56 up to - 4.8. The antileishmanial activities of the cu-SEDDS formulations in terms of IC50 against Leishmania tropica ranged from 0.19 up to 0.37 µg/ml. The minimum inhibitory concentrations (MICs) of these formulations against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Klebsiella pneumoniae were in the range of 48-62 µg/ml. The hemolysis caused by formulations was 1-2%. The spreading potential of the formulations (F1 and F5) over damaged skin model was remarkable. These results suggest that cu-SEDDS further enhanced the broad spectrum antileishmanial and antibacterial profile of curcumin and could be used for the treatment of cutaneous leishmaniasis and its associated secondary infections.


Assuntos
Antibacterianos/farmacologia , Infecções Bacterianas/tratamento farmacológico , Coinfecção/tratamento farmacológico , Curcumina/uso terapêutico , Emulsões/química , Leishmaniose Cutânea/microbiologia , Bactérias/efeitos dos fármacos , Bactérias/patogenicidade , Infecções Bacterianas/parasitologia , Química Farmacêutica , Coinfecção/microbiologia , Composição de Medicamentos , Sistemas de Liberação de Medicamentos , Emulsões/uso terapêutico , Excipientes , Humanos , Leishmaniose Cutânea/complicações , Testes de Sensibilidade Microbiana , Tamanho da Partícula , Pseudomonas aeruginosa/efeitos dos fármacos , Solubilidade , Staphylococcus aureus/efeitos dos fármacos
18.
Yi Chuan ; 41(7): 611-624, 2019 Jul 20.
Artigo em Chinês | MEDLINE | ID: mdl-31307970

RESUMO

Rapid detection of pathogenic microorganisms is key to the epidemiologic identification, prevention and control of disease in the field of public health. PCR-based pathogen detection methods have been widely used because they overcome the time-consuming issues that traditional culture-based methods required including the limited window required by immunological detection. However, the requirement on precision temperature-controlled thermal cyclers severely limits their use in resource-limited areas. The detection methods of pathogenic microorganisms based on isothermal amplification of nucleic acids are free of dependence on high-precision temperature control equipment, but requirements for nucleic acids extraction, amplification and detection must be defined. In recent years, a number of alternative methods for pathogenic microorganism detection have been developed by combining microfluidic technology with nucleic acid isothermal amplification technology. By designing the chip structures, optimizing the injection modes, and utilizing multiple detection and quantitative methods, the integration of pathogen nucleic acid extraction, amplification and detection is achieved. The method provides advantages of less instrument dependence, decreased operator requirements, smaller sample size, and higher automation which are suitable for the rapid detection of pathogenic microorganisms in various environments. In this review, we summarize several microfluidic detection methods based on nucleic acid isothermal amplification for pathogens including amplification principles, injection methods and detection methods. These methods provide more capability for the rapid screening of pathogenic microorganisms which enhances the management of infectious diseases in the field of public health.


Assuntos
Bactérias/isolamento & purificação , Dispositivos Lab-On-A-Chip , Técnicas de Amplificação de Ácido Nucleico , Ácidos Nucleicos/análise , Vírus/isolamento & purificação , Bactérias/patogenicidade , Reação em Cadeia da Polimerase , Vírus/patogenicidade
20.
Microbiol Res ; 226: 1-9, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31284938

RESUMO

Citrus crops have great economic importance worldwide. However, citrus production faces many diseases caused by different pathogens, such as bacteria, oomycetes, fungi and viruses. To overcome important plant diseases in general, new technologies have been developed and applied to crop protection, including RNA interference (RNAi) and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) systems. RNAi has been demonstrated to be a powerful tool for application in plant defence mechanisms against different pathogens as well as their respective vectors, and CRISPR/Cas system has become widely used in gene editing or reprogramming or knocking out any chosen DNA/RNA sequence. In this article, we provide an overview of the use of RNAi and CRISPR/Cas technologies in management strategies to control several plants diseases, and we discuss how these strategies can be potentially used against citrus pathogens.


Assuntos
Citrus/genética , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Doenças das Plantas/genética , Doenças das Plantas/prevenção & controle , Interferência de RNA , Bactérias/genética , Bactérias/patogenicidade , Sistemas CRISPR-Cas , Citrus/microbiologia , Produtos Agrícolas , Fungos/genética , Fungos/patogenicidade , Edição de Genes/métodos , Engenharia Genética , Vírus/genética , Vírus/patogenicidade
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