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1.
Bioresour Technol ; 296: 122336, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31704603

RESUMO

Bauhinia variegate flower (BVF) was supposed to improve silage fermentation due to its abundant active components. Thus, corn stalk and stylo were ensiled with addition of 0, 5% or 10% BVF, and then ensiling characteristics, protein fraction and bacterial community were analyzed after 60-day fermentation. The contents of butyric acid (2.9 vs not detected, 13.2 vs 3.0 g/kg DM in corn stalk and stylo silage, respectively), ammonia-N (100.2 vs 83.2, 110.8 vs 61.9 g/kg total N) and free amino acid (35.6 vs 16.5, 35.0 vs 16.4 g/kg total N) were decreased in 10% BVF treated silages. The bacterial diversity was increased, where the relative abundance of Enterobacter or Clostridium decreased and that of lactic acid producing bacteria such as Lactobacillus, Weissella or Enterococcus increased. It is suggested that BVF could be used to improve fermentation quality and nutrient preservation of high-moisture corn stalk and stylo silage.


Assuntos
Bauhinia , Silagem , Bactérias , Fermentação , Flores , Proteólise , Características de Residência , Zea mays
2.
J Photochem Photobiol B ; 197: 111536, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31326846

RESUMO

The latent utilization of biomaterials that are osteo-conducive in the advancement of healing bone fracture has fascinated extensive consideration. This work includes the synthesis of silver nanoparticles (AgNPs) with the help of a Bauhinia acuminate plant flower extract through an ecofriendly synthetic process without any use of harmful reductants. In the fabrication of AgNPs, Bauhinia acuminate plant flower extract bio constituents acts as both stabilizing and reducing agent. The studies of Fourier transform infrared (FTIR) and X-ray diffraction (XRD) techniques confirmed the formation of AgNPS. TEM images revealed that AgNPs are uniform with average particle size of 17 nm. Further, this work explored if silver nanoparticles (AgNPs) might endorse the osteogenesis and proliferation of mesenchymal stem cells (MSCs) and advance the curing of bone fractures. We also exhibited that the prepared AgNPs could promote the in -vitro osteogenic differentiation and proliferation of MSCs'. Also, the prepared AgNps could stimulate the proliferation of mMSCs at specific concentrations of 6-20 µM. Further, cell viability studies showed that AgNPs exhibited no reduction in mouse mesenchymal stem cell viability at <4 µM. Further, these results indicated the induction effects of AgNPs on osteogenic differentiation and proliferation on MSCs, as well as the advancement of meniscus injury healing.


Assuntos
Bauhinia/química , Nanopartículas Metálicas/química , Osteogênese , Extratos Vegetais/química , Prata/química , Animais , Bauhinia/metabolismo , Diferenciação Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Flores/química , Flores/metabolismo , Consolidação da Fratura/efeitos dos fármacos , Química Verde , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Nanopartículas Metálicas/toxicidade , Camundongos , Microscopia Eletrônica de Transmissão , Osteogênese/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier
3.
Int J Biol Macromol ; 134: 498-506, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31100390

RESUMO

The present investigation determines the extraction and characterization of seed galactomannans from the Leguminosae taxa of Bauhinia vahlii, Delonix elata, and Peltophorum pterocarpum. The seed galactomannans presented a total yield of 30.82, 24.01 and 25.25% with a Man/Gal ratios of 4.21:1, 2.55:1 and 3.03:1 for B. vahlii, D. elata, and P. pterocarpum, respectively exhibiting 1-4 mannose linkages with galactose side chains at C6 position. The galactomannans presented an efficient water holding capacity, solubility and emulsion properties. Intrinsic viscosity from combined Huggins and Kraemer extrapolations are 3.807, 3.424 and 3.331 dl g-1 and the viscosity average molecular weight by Mark-Houwink relationship are 5.29 × 105, 5.29 × 105 and 4.95 × 105 for B. vahlii, D. elata, and P. pterocarpum, respectively. TEM analysis of the polysaccharide showed an aggregated amorphous smooth particles, oval or tubular in shape, branched with compact surfaces. SEM micrographs of the powdered galactomannan indicate well defined amorphous material with pores and cervices on the rough surface. The thermal property studied by DSC and TG-DTA suggests good thermal stability. These findings demonstrate that the seed galactomannan of B. vahlii, D. elata, and P. pterocarpum could be explored as an effective alternative to commercial galactomannans for industrial applications.


Assuntos
Bauhinia/química , Fabaceae/química , Fenômenos Químicos , Cromatografia Gasosa-Espectrometria de Massas , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/ultraestrutura , Sementes/química , Análise Espectral , Termogravimetria
4.
Pharm Biol ; 57(1): 269-279, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31007116

RESUMO

CONTEXT: Bauhinia L. species, including Bauhinia holophylla (Bong.) Steud. (Fabaceae), have traditionally been used to treat diabetes. Bauhinia is a complex botanical genus, and the indiscriminate use of the diverse Bauhinia species is reflected in the experimental divergence of their medicinal potential. OBJECTIVE: The hypoglycaemic and hypolipidaemic effects, molecular mechanism of action and phytochemical properties of an authentic extract of B. holophylla leaves were evaluated. MATERIALS AND METHODS: A phytochemical study of a 70% EtOH extract was performed using FIA-ESI-IT-MS/MSn and HPLC-PAD-ESI-IT-MS. The extract (200 or 400 mg/kg b.w.) was administered for 14 days to streptozotocin-induced diabetic Swiss mice. Glucose tolerance and insulin sensitivity, blood parameters, gene and protein expression, and the in vivo and in vitro inhibition of intestinal glucosidases were assessed. RESULTS: HPLC-PAD-ESI-IT-MS analysis identified flavonoid derivatives of quercetin, myricetin, luteolin and kaempferol. Treatment with 400 mg/kg of the extract reduced blood glucose (269.0 ± 32.4 mg/dL vs. 468.0 ± 32.2 mg/dL for diabetic animals), improved glucose tolerance, decreased cholesterol and triglyceride levels, and increased the mRNA expression of proteins involved in glucogenesis in the liver and muscle, such as PI3-K/Akt, GS, GSK3-ß (ser-9), AMPK and Glut4. The activity of intestinal maltase was inhibited in vitro (IC50: 43.0 µg/mL for the extract compared to 516.4 µg/mL for acarbose) and in vivo. DISCUSSION AND CONCLUSIONS: Treatment with B. holophylla was associated with a marked hypoglycaemic effect through the stimulation of glycogenesis and inhibition of gluconeogenesis and intestinal glucose absorption, without increasing basal insulinaemia.


Assuntos
Bauhinia/química , Glicemia/biossíntese , Diabetes Mellitus Experimental/tratamento farmacológico , Glicogênio Sintase Quinase 3 beta/antagonistas & inibidores , Hipoglicemiantes/uso terapêutico , Extratos Vegetais/uso terapêutico , Animais , Diabetes Mellitus Experimental/sangue , Relação Dose-Resposta a Droga , Hipoglicemiantes/isolamento & purificação , Masculino , Camundongos , Extratos Vegetais/isolamento & purificação , Estreptozocina
5.
Indian J Pharmacol ; 51(1): 25-30, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31031464

RESUMO

OBJECTIVE: Bauhinia purpurea (BP) Linn. (Caesalpiniaceae) is a plant of great medicinal importance and has been used since ancient times for treating many inflammatory conditions including arthritis. This study investigates the anti-arthritic potential of the hydroalcoholic extract from the stem bark of BP. MATERIALS AND METHODS: The anti-inflammatory and anti-arthritic activity of BP at various doses was used to evaluate its anti-inflammatory activity and anti-arthritic activity. Serum of arthritic rats was collected at day 21 for detecting serum cytokines level and to evaluate the effect of BP on its serum level. Furthermore, the safety of BP was evaluated in acute (5 days) and subacute (28 days) toxicity study in rats. RESULTS: There was a significant inhibition (P < 0.01) in paw edema at a different time scale with different doses of BP (50, 100, and 200 mg/kg). BP also demonstrated dose-dependent anti-arthritic activity on all observation days (3, 7, 14, and 21). In addition, there was also a significant decrease (P < 0.01) in oxidative stress markers, circulating pro-inflammatory cytokine (tumor necrosis factor alpha from 45.91 to 37.44, interleukin-1 (IL-1) ß from 18.24 to 16.06, and IL-6 from 69.77 to 58.44) and an increase in anti-inflammatory cytokine (IL-10 from 8.07 to 12.07) levels. BP was found to be safe with an oral LD50 value of >2 g/kg in acute toxicity study and also no toxicological effect was observed in the oral subacute toxicity study. CONCLUSION: This study demonstrates that BP bark possesses anti-arthritic activity potential and confirm its folklore use in the treatment of inflammatory conditions.


Assuntos
Anti-Inflamatórios/uso terapêutico , Artrite Experimental/tratamento farmacológico , Bauhinia , Extratos Vegetais/uso terapêutico , Animais , Anti-Inflamatórios/farmacologia , Artrite Experimental/sangue , Citocinas/sangue , Edema/sangue , Edema/tratamento farmacológico , Masculino , Extratos Vegetais/farmacologia , Ratos Wistar , Testes de Toxicidade Subaguda
6.
J Med Food ; 22(3): 321-323, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30817232

RESUMO

Twenty-five type 2 diabetic volunteer patients (mean age 62 years) were recruited in a quasi-experimental study without a control group to evaluate the effects of Bauhinia forficata Link tea on lipid profiles. Participants drank the tea of 0.4% B. forficata in 200 mL of water twice a day for 3 months. The clinical parameters evaluated were cholesterol and triglycerides (mg/dL), total cholesterol (mg/dL), weight (kg), postprandial glycemia (mg/dL), and glycosylated hemoglobin (HbA1c). For the study period, statistically significant decreases in triglycerides and total cholesterol levels of 48 and 17 mg/dL, respectively, were observed. B. forficata tea as a complementary therapy in type 2 diabetic patients may help to reduce the levels of some lipid profile parameters. Further studies are suggested to evaluate the effect of the tea.


Assuntos
Bauhinia/química , Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemiantes/administração & dosagem , Metabolismo dos Lipídeos/efeitos dos fármacos , Extratos Vegetais/administração & dosagem , Idoso , Glicemia/metabolismo , Colesterol/metabolismo , Diabetes Mellitus Tipo 2/metabolismo , Feminino , Hemoglobina A Glicada/metabolismo , Humanos , Lipídeos/química , Pessoa de Meia-Idade , Fitoterapia , Folhas de Planta/química , Chá/química
7.
Int J Biol Macromol ; 128: 877-884, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30721748

RESUMO

In this study, we describe the characterization of a new lectin, BfL-II, purified from the seeds of Bauhinia forficata, which is distinct, at sequence-level, from the previously reported lectin from the same specie (BfL). In addition, the gene for this lectin was cloned and expressed in Escherichia coli and its antiproliferative activity was evaluated against human breast and colorectal cancer cells (MCF-7 and HT-29, respectively). The treatment with 100 µg/µL of either native or recombinant BfL (nBfL or rBfL) significantly reduced the proliferation of both cancer cell lines (p < 0.01). The inhibition of HT-29 cell proliferation was as high as 82.5% and 93.6% with 100 µg/µL of rBfL-I and nBfL after 24 h, respectively. Therefore, BfL-II presents a promising antiproliferative activity, which may be applied in the development of new anticancer treatments.


Assuntos
Antineoplásicos/metabolismo , Antineoplásicos/farmacologia , Bauhinia/genética , Galectinas/genética , Galectinas/farmacologia , Lectinas de Plantas/genética , Lectinas de Plantas/farmacologia , Sequência de Aminoácidos , Antineoplásicos/química , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Galectinas/química , Expressão Gênica , Células HT29 , Humanos , Células MCF-7 , Modelos Moleculares , Lectinas de Plantas/química , Conformação Proteica
8.
Chemosphere ; 222: 364-370, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30710762

RESUMO

Two recombinant protease inhibitors from Bauhinia bauhinioides, rBbKI (kallikrein inhibitor) and rBbCI (cruzipain inhibitor) were evaluated for insecticidal activity against workers and soldiers of Nasutitermes corniger (order: Isoptera; family: Termitidae) through the inhibitors' effect on the insect's gut enzymes. The inhibitor rBbKI was more effective than rBbCI in inhibiting the termite's gut enzymes. The kallikrein inhibitor showed termiticidal activity in workers with an LC50 of 0.9 mg mL-1 after 4 days. Conversely, rBbKI did not affect the survival of soldiers and rBbCI did not show termiticidal activity against N. corniger. The two inhibitors showed different specificity towards the termite's gut enzymes, representing interesting tools to characterize N. corniger enzymes. The different effects of rBbKI and rBbCI on the termite's enzymes and survival may be linked to slight structural differences between these inhibitors.


Assuntos
Bauhinia/química , Inseticidas/farmacologia , Isópteros/enzimologia , Inibidores de Proteases/farmacologia , Animais , Cisteína Endopeptidases , Humanos , Calicreínas/antagonistas & inibidores , Proteínas de Protozoários/antagonistas & inibidores , Especificidade por Substrato
9.
Acta Crystallogr D Struct Biol ; 75(Pt 1): 56-69, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30644845

RESUMO

Structures of a recombinant Kunitz-type serine protease inhibitor from Bauhinia bauhinioides (BbKI) complexed with bovine trypsin were determined in two crystal forms. The crystal structure with the L55R mutant of BbKI was determined in space group P64 at 1.94 Šresolution and that with native BbKI in the monoclinic space group P21 at 3.95 Šresolution. The asymmetric unit of the latter crystals contained 44 independent complexes, thus representing one of the largest numbers of independent objects deposited in the Protein Data Bank. Additionally, the structure of the complex with native BbKI was determined at 2.0 Šresolution from P64 crystals isomorphous to those of the mutant. Since BbKI has previously been found to be a potent inhibitor of the trypsin-like plasma kallikrein, it was also tested against several tissue kallikreins. It was found that BbKI is a potent inhibitor of human tissue kallikrein 4 (KLK4) and the chymotrypsin-like human tissue kallikrein 7 (KLK7). Structures of BbKI complexed with the catalytic domain of human plasma kallikrein were modeled, as well as those with KLK4 and KLK7, and the structures were analyzed in order to identify the interactions that are responsible for inhibitory potency.


Assuntos
Bauhinia/química , Calicreínas/química , Proteínas de Plantas/química , Tripsina/química , Animais , Bovinos , Cristalografia por Raios X , Humanos , Calicreínas/antagonistas & inibidores , Modelos Moleculares
10.
Nat Prod Res ; 33(23): 3432-3435, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29781298

RESUMO

The inhibitory activity of a Bauhinia forficata tincture (TBF) was investigated against oral microorganism's strains and against a mature oral biofilm. The viability of planktonic cells was analyzed by Minimal Inhibitory and Microbicidal concentrations of TBF. Salivary samples from health volunteers were collected and mixed to form a saliva pool. An aliquot from this pool were seeded on membranes, which were incubated to form biofilm (48 h). The biofilm was treated according to the groups: G1-Chlorhexidine 0.12%; G2-TBF at the highest MMC; G3-Ethanol at the TBF highest MMC. G4 was the growth control. Streptococcus spp. (S) and total microorganisms (TM) from biofilm were counted. TBF was microbicidal against all oral pathogens. G2 was able to reduce the counts of S and TM from biofilm compared to G3 and G4, but less than G1 (p < 0.05). TBF is able to reduce the microbial levels from a mature oral biofilm.


Assuntos
Anti-Infecciosos/isolamento & purificação , Bauhinia/química , Biofilmes/efeitos dos fármacos , Saliva/microbiologia , Anti-Infecciosos/química , Clorexidina/farmacologia , Voluntários Saudáveis , Humanos , Extratos Vegetais/farmacologia , Folhas de Planta/química
11.
Bioprocess Biosyst Eng ; 42(2): 305-319, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30421171

RESUMO

The synthesis of metal nanoparticles by green methods attained enormous attention in recent years due to its easiness, non-toxicity, and eco-friendly nature. In the present study, noble metal nanoparticles such as silver and gold were prepared using an aqueous leaf extract of a medicinal plant, Bauhinia purpurea. The leaf extract performed as both reducing and stabilizing agents for the development of nanoparticles. The formations of silver and gold nanoparticles were confirmed by observing the surface plasmon resonance peaks at 430 nm and 560 nm, respectively, in UV-Vis absorption spectrum. Various properties of nanoparticles were demonstrated using the characterization techniques such as FTIR, XRD, TEM, and EDX. The synthesized silver and gold nanoparticles had a momentous anticancer effect against lung carcinoma cell line A549 in a dose-dependent manner with IC50 values of 27.97 µg/mL and 36.39 µg/mL, respectively. The antimicrobial studies of synthesized nanoparticles were carried out by agar well diffusion method against six microbial strains. Silver and gold nanoparticles were also showed high antioxidant potentials with IC50 values of 42.37 µg/mL and 27.21 µg/mL, respectively; it was measured using DPPH assay. Additionally, the nanoparticles were observed to be good catalysts for the reduction of organic dyes.


Assuntos
Anti-Infecciosos/química , Antineoplásicos/química , Antioxidantes/química , Bauhinia/química , Nanopartículas Metálicas/química , Folhas de Planta/química , Células A549 , Catálise , Ouro/química , Química Verde , Humanos , Concentração Inibidora 50 , Tamanho da Partícula , Extratos Vegetais/química , Plantas Medicinais/química , Prata/química , Espectroscopia de Infravermelho com Transformada de Fourier , Ressonância de Plasmônio de Superfície , Difração de Raios X
12.
Phytother Res ; 33(1): 90-106, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30281176

RESUMO

This study evaluated the effects of flavonoid-rich fraction from Bauhinia forficata leaves (FRF-BF), against intestinal toxicity induced by irinotecan. The leaves of this plant are used like tea in Brazilian folk medicine, and it is rich in flavonoids, mainly kaempferitrin. First, the chemopreventive effects of FRF-BF and kaempferitrin were evaluated in intestinal cells (IEC-6 cells) exposed to irinotecan. Next, the effects were evaluated against irinotecan-induced mucositis in mice. Lastly, melanoma was induced in C57BL/6 mice to evaluate FRF-BF interference on irinotecan antitumor activity. The results showed that FRF-BF and kaempferitrin exert no cytotoxic effects in IEC-6 cells and confirmed that pretreatment with FRF-BF and kaempferitrin displays chemoprotective effects against cytotoxicity induced by irinotecan. Interestingly, the FRF-BF (100 mg/kg, p.o) reduced the intestinal motility in mice and attenuated parameters linked to irinotecan-induced intestinal mucositis, including diarrhea, histological damage, depletion of duodenal GSH, amount of TNF-α, and MPO activity in the small intestine. Also, FRF-BF does not interfere in the antitumor activity of irinotecan and exerted antitumoral activity in murine melanoma. In conclusion, FRF-BF (100 mg/kg, p.o) presents promising pharmacological potential to prevent and attenuate the severity of intestinal mucositis during chemotherapy treatment, related to the presence of kaempferitrin.


Assuntos
Bauhinia/química , Flavonoides/química , Irinotecano/efeitos adversos , Extratos Vegetais/química , Folhas de Planta/química , Animais , Irinotecano/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL
13.
Nat Prod Res ; 33(17): 2574-2578, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29620448

RESUMO

Several Bauhinia species are widely used in Southern South America in the treatment of infections, pain and several diseases including diabetes. Flavonoid compounds based on quercetin and kaempferol glycoside derivatives are believed to be responsible for their therapeutic properties. To investigate this, we have studied two native species from Argentina: B. uruguayensis (BU) and B. forficata subsp. pruinosa (BF). We have analyzed the major polyphenol components in hydro-methanolic extracts of leaves, by high performance liquid chromatography tandem mass spectrometry. Chromatographic analysis yielded five main compounds in BF, corresponding to rutinosides and rhamnosides derivatives of kaempferol and quercetin, which are considered chemotaxonomic markers and responsible for antioxidant activity. The presence of kaempferitrin, an antidiabetic agent, has been confirmed. In extracts of BU, four major compounds were identified as rhamnosides and galloyl derivates from quercetin and kaempferol. One of these compounds, quercitrin-3-rhamnoside may confer anti-inflammatory and analgesic properties to BU extracts.


Assuntos
Bauhinia/química , Flavonoides/análise , Glicosídeos/análise , Analgésicos/isolamento & purificação , Anti-Inflamatórios/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Flavonoides/química , Glicosídeos/química , Hipoglicemiantes/isolamento & purificação , Quempferóis/análise , Extratos Vegetais/química , Folhas de Planta/química , Quercetina/análogos & derivados , Quercetina/análise , Espectrometria de Massas em Tandem
14.
Microsc Res Tech ; 82(4): 345-351, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30575198

RESUMO

Bauhinia forficata is a medicinal plant that has flavonoid components with hypoglycemic, antioxidant, hepatoprotective, antibacterial, antiviral and anti-inflammatory action. Aim of this study is to evaluate the action of B. forficata alcoholic extract in the male genital system of adult male Wistar rats. For that, 20 adult male Wistar rats were distributed into two experimental groups: the B. forficata group, receiving B. forficata alcoholic extract (0.1 ml/10 g body weight/day) on alternate days, and the control group, receiving just the vehicle for 30 days straight both via gavage. On the 31st day, the animals were euthanized, and the testis and epididymis were collected for histopathological, biochemical, morphometric, and sperm count analysis. Mass spectrometry identified new compounds in the extract: trans-caffeic acid, liquiritigenin, gallocatechin, and 2,4,6-trihydroxyphenanthren-2-glycoside. Biochemical analysis showed higher total cholesterol levels in the testis and lower malondialdehyde levels in the testis and epididymis, in the B. forficata group. The mast cell count showed a reduction in degranulated mast cells in the caput region of the epididymis, in the B. forficata group. The luminal compartment of the caput and the epithelial of the epididymis cauda were reduced, whereas the stromal region of the epididymis caput was increased in the B. forficata group, compared with the control group. The testicular tissue was less impaired, considering that all the histological analyses were similar to the control. We believe that B. forficata alcoholic extract in the male genital system showed antioxidant action, especially in the epididymal tissue.


Assuntos
Antioxidantes/farmacologia , Bauhinia/química , Epididimo/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Extratos Vegetais/farmacologia , Testículo/metabolismo , Animais , Degranulação Celular/efeitos dos fármacos , Colesterol/análise , Masculino , Malondialdeído/análise , Mastócitos/citologia , Fitoterapia , Ratos , Ratos Wistar
15.
Complement Ther Med ; 41: 1-9, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30477823

RESUMO

BACKGROUND: There are several ways to identify medicinal power of phytoconstituents, such as in silico evaluations. Furthermore, ethnopharmacological researches are important alternatives for the identification of plants with medicinal potential. Significantly, medicinal plants are widely used by persons with Diabetes mellitus (DM) to treat manifestations of this syndrome. OBJECTIVES: i) to investigate the use of medicinal plants for individuals with DM and their health profile; ii) to evaluate in silico possible antidiabetic activities for main phytoconstituents of the commonly used plants. METHODS: A questionnaire was used to measure consumption of medicinal plants. The Prediction of Activity Spectra for Substances (PASS) platform was employed to perform in silico evaluations. In silico predictions for antidiabetic activities were performed with the main compounds identified in a literature review which focused on the more utilized plants. RESULTS: We interviewed 105 persons with DM, most them women (73.34%). Overall mean age was 59.35 years, and 97.14% of them were diagnosed with type 2 DM. An evaluation of the routine exams of the interviewees showed that they have a poor metabolic control. Among the interviewees, 67.62% confirmed the use medicinal plants. Main forms of consumed plant preparation were infusion of leaves and in association with mate (a typical beverage of southern Brazil). Most interviewees consume five or more cups of infusion per day, and when consumed with the mate, 1.73 liters per day. Forty-six medicinal plants were mentioned, and cow's paw (Bauhinia) and jambolan (Syzygium cumini) were the most used. The main informed objective for the plant use was blood glucose control (69.01%). The PASS analysis presented six phytoconstituents with high antidiabetic prediction, especially, vicenin-2, the main phytochemical identified in Passiflora genus (Pa = 0.822). CONCLUSION: Our data show that persons with DM use many plants as a complementary treatment to the traditional medicine. Moreover, part of these plants presented phytoconstituents with antidiabetic potential. These data can serve as a basis for future investigations, with the objective of exploring in vitro and in vivo antidiabetic effects of these plants and its compounds.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemiantes/uso terapêutico , Magnoliopsida/química , Aceitação pelo Paciente de Cuidados de Saúde , Compostos Fitoquímicos/uso terapêutico , Fitoterapia , Preparações de Plantas/uso terapêutico , Apigenina/farmacologia , Apigenina/uso terapêutico , Bauhinia/química , Glicemia/metabolismo , Brasil , Diabetes Mellitus Tipo 2/sangue , Feminino , Glucosídeos/farmacologia , Glucosídeos/uso terapêutico , Humanos , Hipoglicemiantes/farmacologia , Masculino , Medicina Tradicional , Pessoa de Meia-Idade , Passiflora/química , Compostos Fitoquímicos/farmacologia , Preparações de Plantas/farmacologia , Plantas Medicinais/química , Syzygium/química
16.
Biosci. j. (Online) ; 34(6 Supplement 1): 120-130, nov./dec. 2018.
Artigo em Inglês | LILACS | ID: biblio-968877

RESUMO

The pata-de-vaca (Bauhinia divaricata L.) tree is a species widely distributed in Brazil, it has a high ornamental and economic value. Its leaves are used in renal inflammations, such as diuretic, hypoglycemic, being considered a medicinal plant of popular use. The research was carried out in a greenhouse belonging to the Laboratório de Ecologia Vegetal, at the Centro de Ciências Agrárias (CCA), from Universidade Federal of Paraíba (UFPB). The objective was to evaluate the morphophysiological characteristics of B. divaricata plants at different water regimes. The experimental design was completely randomized with five water treatments 1 (100% control), 2 (80%), 3 (60%), 4 (40%) and 5 (20%) of the container capacity. The height, number of leaves and stem diameter were evaluated weekly. At the end of the experiment the plants had their organs separated and taken to the greenhouse to obtain the dry matter of the leaves, stem, roots and total dry matter, and also, biomass allocation in the leaves, stem and roots. Plant height did not differ statistically between the treatments. The number of leaves, diameter, leaf, stem and root allocation and root and shoot dry matter were higher under water availability (100% of container capacity). For the production of B. divaricata L. seedlings water regime should be 100% and 80% of the container capacity, but the seedlings grow satisfactorily.


A pata-de-vaca (Bauhinia divaricata L.) é uma espécie arbórea, amplamente distribuída no Brasil, de alto valor ornamental e econômico. Suas folhas são utilizadas nas inflamações renais, como diuréticas, hipoglicemiantes, sendo considerada uma planta medicinal de uso popular. A pesquisa foi desenvolvida em casa de vegetação pertencente ao Laboratório de Ecologia Vegetal, Centro de Ciências Agrárias (CCA), Universidade Federal da Paraíba (UFPB). O Objetivo foi avaliar as características morfofisiológicas em plantas de Bauhinia divaricata em diferentes regimes hídricos. O delineamento experimental foi inteiramente casualizado com cinco tratamentos hídrico 100%, 80%, 60%, 40% e 20% da capacidade de pote. Avaliou-se, semanalmente a altura, número de folhas e diâmetro do caule. Ao final do experimento as plantas tiveram seus órgãos separados e levados à estufa para obtenção da matéria seca das folhas, caule, raízes e massa seca total, e ainda, alocação de biomassa nas folhas, caule e raízes. A altura de plantas não diferiu estatisticamente entre os tratamentos, o número de folhas, diâmetro, alocação de folhas, caules e raízes e massas seca da raiz e parte aérea foram maiores sob maior disponibilidade de água (100% da capacidade do recipiente). Para a produção de mudas de B. divaricata L. o regime hídrico deve ser de 100% e 80% da capacidade do recipiente, porém as mudas crescem satisfatoriamente.


Assuntos
Plantas , Água , Biomassa , Bauhinia , Fabaceae
17.
J Trace Elem Med Biol ; 50: 146-153, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30262272

RESUMO

To develop novel nanoformulated insecticides and antimicrobials, herein we produced Ag nanoparticles (AgNPs) using the Bauhinia acuminata leaf extract. This unexpensive aqueous extract acted as a capping and reducing agent for the formation of AgNPs. We characterized B. acuminata-synthesized AgNPs by UV-vis and FTIR spectroscopy, XRD and TEM analyses. UV-vis spectroscopy analysis of B. acuminata-synthesized AgNPs showed a peak at 441.5 nm. FTIR shed light on functional groups from the phytoconstituents involved in nanosynthesis. XRD of B. acuminata-synthesized AgNPs suggested a face-centered cubic structure, with a highly crystalline nature. TEM of B. acuminata-synthesized AgNPs revealed mean size of 25 nm, with round shape. AgNPs tested at 60 µg/mL inhibited the growth of 5 bacteria and 3 fungal pathogens. In the insecticidal assays on important mosquito species, LC50 of the aqueous extract of B. acuminata leaves on the larvae of Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus were 204.07, 226.02, and 249.24 µg/mL, respectively. The B. acuminata-synthesized AgNPs exhibited higher larvicidal efficacy, with LC50 values of 24.59, 27.19, and 30.19 µg/mL, respectively. Therefore, herein we developed a single-step, reliable, inexpensive, and environmentally non-toxic synthesis process to obtain AgNPs with high bioactivity against pathogens and vectors. Given the effective antimicrobial and larvicidal activity, nanoparticles fabricated using plant extracts and extremely low concentrations of trace elements, such as silver, can be exploited for multipurpose activities. Our results pointed out that B. acuminata-synthesized AgNPs have a promising potential in antimicrobial food packaging, as well as a foliar spray to control plant pathogens in the field, and to synergize the efficacy of fungicidal and larvicidal formulations.


Assuntos
Anti-Infecciosos/química , Bauhinia/química , Inseticidas/química , Nanopartículas Metálicas/química , Compostos Fitoquímicos/química , Extratos Vegetais/química , Prata/química , Animais , Espectroscopia de Infravermelho com Transformada de Fourier
18.
Int J Biol Macromol ; 119: 811-820, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30071232

RESUMO

Lectins are proteins of non-immune origin present throughout all kingdoms of life. They are capable of binding to specific carbohydrates reversibly, thus performing several biological roles. Plant lectins are the most studied ones, with hundreds of isolated and characterized hemagglutinins. Most of the known lectins have been isolated from plants belonging to family Leguminosae, which includes genus Bauhinia. This genus comprises over 300 species located in the tropical zones of the planet, where these are utilized in folk medicine because of their numerous medicinal effects, such as anti-inflammatory and antidiabetic actions. Despite being studied for over fifty years, the literature regarding Bauhinia hemagglutinins is scarce, describing just ten proteins isolated from seven different species. Structurally as well as biophysically, there is great similarity among all the known Bauhinia lectins, which may classify them as chemotaxonomic markers; however, the carbohydrate-binding sites and further specificities are unique for each of these proteins. The activities identified for these lectins include growth inhibition in cancer cell lines, cell marking, anti-inflammatory and insecticidal effects, which are just a few among their various other activities of high economic importance. Besides their versatility, four recombinant Bauhinia lectins have already been successfully expressed in heterologous microbial systems, further suggesting that these proteins could serve as promising biotechnological products in future.


Assuntos
Bauhinia/química , Lectinas de Plantas/química , Lectinas de Plantas/farmacologia , Biotecnologia , Fenômenos Químicos , Lectinas de Plantas/isolamento & purificação , Proteínas Recombinantes , Relação Estrutura-Atividade
19.
Mater Sci Eng C Mater Biol Appl ; 91: 436-444, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-30033274

RESUMO

Nanobiotechnology has become a newly evolving field of interest in biomedical applications due to its biocompatibility and non-toxic nature towards the environment. Metal and metal oxide nanoparticles have been widely used as an antibacterial agent due to the emergence of antibiotic resistant pathogens, which leads to the outbreak of infectious diseases. In the present paper, biogenic synthesis of magnesium oxide (MgO) nanoflakes is reported by using Bauhinia purpurea leaf extract through alkaline precipitation method along with its detailed characterization. The average size of synthesized nanoflakes was found to be around 11 nm. Electron microscopy was used to investigate the morphology of the MgO nanoflakes. Additionally, the presence of antioxidants, phenolics and flavonoids in B. purpurea leaf extract has been studied by using different assays, which suggested the efficacy of leaf extract as a potential reducing agent for MgO nanoflakes synthesis. Antibacterial activity of synthesized MgO nanoflakes was investigated against Staphylococcus aureus, a gram positive bacteria known to cause various infections in humans. Results suggested the high efficacy of MgO nanoflakes as a potential antibacterial agent against S. aureus at meager dose size (250 µg/ml) and possible mode of action was investigated through surface morphology analysis of bacterial cells by field emission scanning electron microscopy.


Assuntos
Antibacterianos/farmacologia , Bauhinia/química , Óxido de Magnésio/farmacologia , Nanopartículas/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Staphylococcus aureus/efeitos dos fármacos , Antioxidantes/farmacologia , Contagem de Colônia Microbiana , Flavonoides/análise , Depuradores de Radicais Livres/farmacologia , Testes de Sensibilidade Microbiana , Viabilidade Microbiana/efeitos dos fármacos , Nanopartículas/ultraestrutura , Oxirredução , Fenóis/análise , Espécies Reativas de Oxigênio/metabolismo , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier , Staphylococcus aureus/citologia , Difração de Raios X
20.
Drug Dev Res ; 79(4): 157-164, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29732583

RESUMO

Hit, Lead & Candidate Discovery The acetic acid-induced writhing and hot plate models in mice were utilized to determine the analgesic effect of epicatechin gallate (ECG) isolated from Bauhinia hookeri. The anti-inflammatory activity of ECG was determined using carrageenan-induced paw edema model. The pro-inflammatory mediators (PGE2 , TNF-α, IL-1ß, and IL-6) were estimated in the plasma of different treatment groups. ECG was tested at doses of 100, 200, and 400 mg/kg p.o. and diclofenac sodium was used as a standard drug (100 mg/kg) in all experiments. ECG significantly (p < .001) suppressed the writhing response in mice. The inhibition percentages were 32, 52, and 62%, at the tested doses of ECG, respectively as compared to the positive control group receiving acetic acid only. Furthermore, ECG significantly (p < .001) increased the reaction time in hot plate model. The maximum analgesic effect was evident after 120 min. ECG demonstrated a significant anti-inflammatory activity as evidenced by the inhibition of carrageenan-induced paw edema (46, 50, and 58%, at the tested doses, respectively). This effect was persistent all over the experimental period. ECG produced a significant (p < .001) reduction in plasma PGE2 (by 27, 38, and 50%), TNF-α (15, 33, and 41%), IL-1ß (17, 25, and 33%), and IL-6 (22, 32, and 43%), at the tested doses, respectively. This study supports the use of ECG as both analgesic and anti-inflammatory agent.


Assuntos
Anti-Inflamatórios/farmacologia , Bauhinia/química , Catequina/análogos & derivados , Medição da Dor/efeitos dos fármacos , Analgésicos/farmacologia , Animais , Catequina/isolamento & purificação , Catequina/farmacologia , Catequina/toxicidade , Diclofenaco/farmacologia , Dinoprostona/sangue , Relação Dose-Resposta a Droga , Interleucina-1beta/sangue , Interleucina-6/sangue , Masculino , Camundongos , Fator de Necrose Tumoral alfa/sangue
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