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1.
Sci Total Environ ; 857(Pt 3): 159733, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36306848

RESUMO

Benzophenone-3 (BP3) is an organic UV filter widely used in the commercial formulations of various personal care products. It has been detected ubiquitously in the environment and human tissues. Recently, BP3-induced neurotoxicity has been identified as the main health risk to humans and aquatic organisms. However, most research has been focused on embryonic development, and few studies explore chronic lifetime exposure. In the present study, we evaluated the neurotoxicity of lifetime exposure to an environmentally relevant concentration of BP3 in zebrafish. Our findings revealed that continuous BP3 exposure at 10 µg/L (0.04 µM) from 6 h post fertilization (hpf) to adulthood at 5 months led to female-biased social behavioral deficits and learning and memory impairment. These neurobehavioral effects were characterized by decreased prosocial activities in the social preference test and mirror biting assay, and reduced learning and memory in a T-maze test. Furthermore, these effects were accompanied by female-specific decreases in brain weight and brain dopamine concentration, female-biased decrease of neurogenesis in the telencephalon as well as female-specific increases in apoptotic cells and expression levels of genes and proteins related to the apoptosis pathway in the brain. Our results suggest that BP3-induced social behavior and learning/memory deficits are correlated to the cell loss in the telencephalon region of the zebrafish brain.


Assuntos
Benzofenonas , Peixe-Zebra , Animais , Humanos , Feminino , Adulto , Benzofenonas/toxicidade , Benzofenonas/metabolismo , Comportamento Social , Cognição
2.
J Hazard Mater ; 443(Pt A): 130198, 2023 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-36279648

RESUMO

Laccases are multicopper oxidases of significant importance for the degradation of phenolic pollutants. Because of the inherent defects of natural laccases in practical applications, herein, we discovered highly effective and non-cytotoxic laccase-like metallo-nanofibers based on the supramolecular assembly of single unnatural amino acid, benzophenone-alanine (BpA), in combination with copper ions. Structural analysis revealed that the catalytic BpA-Cu nanofibers possess a Cu(I)-Cu(II) electron transfer system similar to that in natural laccase. Our BpA-Cu nanofibers exhibit 4 times higher substrate affinity and 24% higher catalytic efficiency than the well-known high-performance industrialized laccase (Novozym 51003) in 2,4-dichlorophenol degradation. In addition, the BpA-Cu nanofibers were demonstrated to be stable (>75% residual activity) in long-term storage at a wide range of pH, ionic strength, temperature, ethanol, and water sample, and to be readily recovered for pollutant degradation, keeping 83% of the laccase activity after 10 catalytic recycles. Remarkably, the nanofibers displayed a wide substrate spectrum, detecting and degrading a variety of phenolic pollutants with high activity than other laccase mimics reported in the literature. Furthermore, the biocompatibility of the material was proved with cultured cells. These findings demonstrated the potential of BpA-Cu nanofibers in mimicking laccases for environmental remediation.


Assuntos
Poluentes Ambientais , Lacase , Lacase/metabolismo , Cobre/química , Alanina , Fenóis/química , Benzofenonas
3.
ACS Appl Mater Interfaces ; 14(47): 52579-52598, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36380598

RESUMO

Patients suffering diabetic bone defects still need some new and effective strategies to achieve enhanced prognostic effects. Although medical implants are the common treatment of bone defects, the excessive oxidative stress and high risk of bacterial infection in diabetes mellitus lead to a higher risk of implant failure. To improve the healing ability of diabetic bone defects, herein, polyetheretherketone (PEEK) was modified through a developed layer-by-layer (LBL) construction strategy to obtain multifunctional PEEK (SP@(TA-GS/PF)*3) by the assembly of tannic acid (TA), gentamicin sulfate (GS) and Pluronic F127 (PF127) on the basis of prepared porous PEEK through sulfonation (SPEEK). The prepared SP@(TA-GS/PF)*3 exhibited sustained antimicrobial activity and enhanced the differentiation of osteoblast (MC3T3-E1) for needed osteogenesis. Moreover, SP@(TA-GS/PF)*3 scavenged excessive oxidative stress to promote the growth of H2O2 damaged HUVEC with enhanced secretion of VEGF for neovascularization. In addition, the remarkable in vivo outcomes of angiogenesis and osseointegration were revealed by the subcutaneous implant model and bone tissue implant model in diabetic rats, respectively. The in vitro and in vivo results demonstrated that modified PEEK with multifunction can be an attractive tool for enhancing bone integration under diabetic conditions, underpinning the clinical application potential of modified implants for diabetic osseointegration.


Assuntos
Antioxidantes , Diabetes Mellitus Experimental , Ratos , Animais , Antioxidantes/farmacologia , Peróxido de Hidrogênio/farmacologia , Diabetes Mellitus Experimental/tratamento farmacológico , Benzofenonas/farmacologia , Cetonas/farmacologia , Polietilenoglicóis/farmacologia , Osseointegração , Osteogênese , Osso e Ossos , Antibacterianos/farmacologia , Propriedades de Superfície
4.
Mar Pollut Bull ; 185(Pt A): 114313, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36327937

RESUMO

Benzophenone-3 (BP-3), 4-methyl-benzylidene camphor (4-MBC) and 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) are commonly used organic ultraviolet (UV) filters and are frequently detected in water environments. In the present study, we studied the potential adverse impacts of UV filter exposures in Ruditapes philippinarum by investigating transcriptomic profiles and non-specific immune enzyme activities. Transcriptome analysis showed that more genes were differentially regulated in EHMC-treated group, and down-regulated genes (2009) were significantly more than up-regulated ones (410) at day 7. Function annotation revealed that pathways "immune system", "cell growth and death" and "infectious diseases" were significantly enriched. Generally, combined qPCR and biochemical analyses demonstrated that short-term exposure to low dose of UV filters could activate immune responses, whereas the immune system would be restrained after prolonged exposure. Taken together, the present study firstly demonstrated the immunotoxicology induced by BP-3, 4-MBC and EHMC on R. philippinarum, indicating their potential threats to the survival of marine bivalves.


Assuntos
Bivalves , Transcriptoma , Animais , Bivalves/genética , Perfilação da Expressão Gênica , Benzofenonas/análise
5.
Anal Chem ; 94(45): 15586-15594, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36318671

RESUMO

Hydrogels are incorporated into microfluidic devices to provide enhanced functionality by enabling processes such as enzyme immobilization, sample enrichment, and ionic current rectification. However, in the microfluidic devices with the commonly used material poly(dimethylsiloxane) (PDMS), hydrogels are very difficult to polymerize in situ in an ambient atmosphere because of the high oxygen concentration in PDMS. Even with very high (1.8%) photoinitiator concentrations, the polymerized hydrogel does not completely fill the microchannel. Here, we report a facile and broadly applicable protocol that utilizes microchannel pretreatment with 20% benzophenone in acetone to provide a hydrogel plug that completely fills the microchannel cross section by consuming the oxygen in the PDMS substrate near the microchannel wall. Both negatively charged and neutral hydrogels are polymerized from monomer solutions that utilize the photoinitiator/solvent combinations of VA-086 in water and benzophenone or IRG in DMSO. The photoinitiators were tested at different concentrations and in devices with different levels of oxidation. The hydrogel morphology is characterized using phase contrast microscopy and is related to the hydrogel's performance for concentration enrichment and ionic current rectification. A novel method is employed to confine the precursor solution in desired locations so that a photomask is not required for the spatial control of the plug location. Among six hydrogel formulations, at 100 V, the best current rectification factor obtained is ∼600 and the best analyte enrichment achieved is ∼120-fold in 5 min. This method provides a rapid and simple approach to increase the capabilities of PDMS microfluidic devices through improved polymerization of nanoporous hydrogels.


Assuntos
Dimetilpolisiloxanos , Hidrogéis , Hidrogéis/química , Polimerização , Dimetilpolisiloxanos/química , Benzofenonas , Oxigênio
6.
Sci Rep ; 12(1): 19070, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36351981

RESUMO

We conducted a six-month clinical follow-up on computer-aided design/computer-aided manufacturing-fabricated molar polyetheretherketone PEEK crowns to investigate their therapeutic effect. Only the PEEK crowns were examined as our study focused on short-term clinical evaluation of the new PEEK material. Twenty-three cases of PEEK crowns placed on the molars of 20 subjects (7 males and 13 females, mean age: 60.6 ± 14.2 years) were included in the study. The evaluation items were the condition of the crowns at the time of cementation and after six months, patient satisfaction, masticatory ability, and occlusal force. Mann-Whitney U tests with a significance level of 5% were used to examine the difference in glucose concentration by masticatory ability, occlusal pressure, and occlusal force, with and without PEEK crowns. The occlusion, margin fit, and contact of all 23 cases at the time of cementing were good. Six months after cementation, there was no crown desorption, fracture or crack, and prosthodontics was not needed in the 22 cases (one patient dropped out). No wear of the dental antagonist was observed. Patient satisfaction was generally high. There was no significant difference in masticatory ability between the groups with and without PEEK crowns. The subject's occlusal force was within the normal range. PEEK crowns used on molars can replace metal crowns and hold promise for an appropriate and effective treatment.


Assuntos
Coroas , Polímeros , Masculino , Feminino , Humanos , Pessoa de Meia-Idade , Idoso , Seguimentos , Benzofenonas , Cetonas , Desenho Assistido por Computador , Polietilenoglicóis
7.
Angew Chem Int Ed Engl ; 61(49): e202213065, 2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36250269

RESUMO

Colloidal quantum dots (QDs) can photocatalyze diverse organic reactions. However, reported QD-photocatalysts often contain highly-toxic elements Cd or Pb, and have not surpassed prototypical transition-metal complexes in terms of their photoredox power or excited-state energy. Here we report low-toxicity ZnSe/ZnS core/shell QDs as potent visible photocatalysts to drive challenging organic transformations. To overcome the limitation of short excited-state lifetime of the QDs, we functionalize their surfaces with benzophenone ligands which can rapidly extract electrons from photoexcited QDs and sustain long-lived charge-separated states. The benzophenone anions function as potent electron relay to drive dehalogenation of aryl chlorides and additive-free polymerization of acrylates. Alternatively, the QDs are functionalized with biphenyl ligands to store energy in long-lived, energetic triplets, enabling [2+2] homo-cycloaddition of styrene and cycloaddition of carbonyls with alkenes.


Assuntos
Pontos Quânticos , Pontos Quânticos/toxicidade , Compostos de Zinco , Sulfetos , Benzofenonas
8.
Ann Afr Med ; 21(3): 217-222, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36204906

RESUMO

Background: Abutment surfaces are being designed to promote gingival soft tissue attachment and integration. This confirms implant survival for long term by forming a seal around the prosthetics. Objectives: This study was done to compare the biocompatibility of three implant abutments: titanium uncoated, Ti-nitride coated, and modified polyetheretherketone (PEEK) with human gingival keratinocytes. Materials and Methods: The titanium-uncoated, titanium-nitride-coated, and modified PEEK discs (13 mm × 3 mm) were fabricated and compared with uncoated polyester cell culture discs, which were used as controls. These three implant abutments were evaluated for biocompatibility with respect to human gingival keratinocytes for viability, morphology, proliferation, and migration by scanning electron microscopy imaging and scratch wound healing assays. Measurements of roughness show changes between the investigated surfaces. Results: Keratinocytes cultured on all examined surfaces indicated adhesion and attachment. An assay of cell viability showed no substantial variances among the groups. The modified PEEK surface showed greater cell proliferation and migration among the three abutment materials. Conclusion: All three abutment material surface types showed similar epithelial biological responses. However, modified PEEK material showed the highest biocompatibility.


Résumé Contexte: Les surfaces des piliers sont conçues pour favoriser la fixation et l'intégration des tissus mous gingivaux. Cela confirme l'implantation survie à long terme en formant un joint autour des prothèses. Objectifs : Cette étude a été réalisée pour comparer la biocompatibilité de trois Piliers Implantaires : titane non revêtu, revêtu de nitrure de titane et polyétheréthercétone modifié (PEEK) avec des kératinocytes gingivaux humains. Matériaux et Méthodes: les disques PEEK non revêtus de titane, revêtus de nitrure de titane et modifiés (13 mm × 3 mm) ont été fabriqués et comparés à des disques de culture cellulaire en polyester non revêtus, qui ont été utilisés comme témoins. Ces trois piliers implantaires ont été évalués pour biocompatibilité vis-à-vis des kératinocytes gingivaux humains pour la viabilité, la morphologie, la prolifération et la migration par balayage électronique l'imagerie microscopique et les tests de cicatrisation des plaies. Les mesures de rugosité montrent des changements entre les surfaces étudiées. Résultats: Les kératinocytes cultivés sur toutes les surfaces examinées ont indiqué une adhérence et une fixation. Un test de viabilité cellulaire n'a montré aucune écarts entre les groupes. La surface PEEK modifiée a montré une plus grande prolifération et migration cellulaire parmi les trois matériaux de pilier. Conclusion: Les trois types de surfaces de matériaux de pilier ont montré des réponses biologiques épithéliales similaires. Cependant, le matériau PEEK modifié a montré la biocompatibilité la plus élevée. Mots-clés: Biocompatibilité, implant, kératinocytes, prothèses, microscopie électronique à balayage.


Assuntos
Queratinócitos , Titânio , Benzofenonas , Humanos , Cetonas , Poliésteres , Polietilenoglicóis , Polímeros , Propriedades de Superfície , Titânio/toxicidade
9.
Anal Chim Acta ; 1231: 340405, 2022 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-36220296

RESUMO

Phthalic acid esters (PAEs) are considered endocrine disruptors and potential carcinogens. Consequently, efficient and accurate environmental monitoring of trace levels of these organic pollutants is necessary to protect the population against their hazardous effects. Passive sampling techniques have gained notoriety for environmental monitoring and have been proven highly sensitive to temporal variations. This study developed a miniaturized passive sampling device (MPSD) based on hollow fiber liquid-phase microextraction (HF-LPME). The devices were calibrated in the laboratory using an automated calibration system. The results demonstrated the first-order uptake ranges for Diethyl phthalate (DEP), Diisobutyl phthalate (DiBP), Dibutyl phthalate (DBP), Benzyl butyl phthalate (BBP) and Bis(2-ethylhexyl phthalate) (DEHP) between 30 min and 24 h with sampling rates equivalent to 0.009; 0.021; 0.033; 0.085 and 0.003 mL h-1 respectively (R2 between 0.88 and 0.99). The calibrated devices were deployed in 12 marginal lagoons, stretching approximately 330 km along the main river. The extracts recovered from the devices were analyzed by gas chromatography (GC), resulting in the identification and quantification of DEP (0.697-13.7 ng L-1), DiBP (0.100-4.43 ng L-1), DBP (0.014-1.21 ng L-1), BBP (0.218-5.67 ng L-1), and DEHP (0.002-2.24 ng L-1). Despite being frequently identified, DEHP concentrations were well below the maximum established limits, revealing a good water quality in terms of the target PAEs. In contrast, screening the extracts using GCxGC was possible to detect other hazardous pollutants such as pesticides, drugs, and their metabolites. The described device was effective and reliable, providing accurate PAE measurements following short exposure periods. In this sense, its deployment during emergency operations, such as accidental discharges of industrial effluents into natural waters, could continuously and cost-effectively monitor water quality.


Assuntos
Dietilexilftalato , Disruptores Endócrinos , Poluentes Ambientais , Microextração em Fase Líquida , Praguicidas , Ácidos Ftálicos , Benzofenonas , Ácidos Borônicos , Carcinógenos/análise , Celulose , Dibutilftalato/análogos & derivados , Dibutilftalato/análise , Disruptores Endócrinos/análise , Monitoramento Ambiental , Poluentes Ambientais/análise , Ésteres/análise , Etilaminas , Praguicidas/análise , Ácidos Ftálicos/análise
10.
Molecules ; 27(20)2022 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-36296651

RESUMO

Two new benzophenone glycosides, hypersens A and B, along with four known compounds, (S)-(+)-5,7-dihydroxy-2-(1-methylpropyl) chromone (3), 5,7-dihydroxy-2-isopropylchromone (4), urachromone B (5), and 3-8'' bisapigenin (6), were isolated from Hypericum seniawinii. The structures of new compounds (1 and 2) were elucidated according to comprehensive spectroscopic data analyses. The absolute configurations of 1 and 2 were determined by electronic circular dichroism (ECD) calculations. All isolated compounds were evaluated for their neuroprotective effect using corticosterone-induced PC12 cell injury. In addition, compounds 1-6 were evaluated for their anti-inflammatory activity in lipopolysaccharide-induced RAW 264.7 cells. Compound 6 was a biflavonoid and significantly inhibited the production of nitric oxide with an IC50 value of 11.48 ± 1.23 µM.


Assuntos
Biflavonoides , Hypericum , Fármacos Neuroprotetores , Animais , Hypericum/química , Cromonas/farmacologia , Cromonas/química , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/química , Óxido Nítrico , Lipopolissacarídeos , Corticosterona , Benzofenonas/química , Glicosídeos/farmacologia , Glicosídeos/química , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/química , Estrutura Molecular
11.
Environ Pollut ; 315: 120405, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36228842

RESUMO

Many environmental phenols, such as bisphenols, benzophenones and parabens, are known as endocrine disruptors and can adversely affect human health. However, the knowledge of human exposure to common environmental phenols in Chinese rural areas is insufficient. In this context, 181 urine samples were collected from participants in a rural area in Northwest China and were analyzed for nine bisphenols, three benzophenones and four parabens. Bisphenol A (BPA), bisphenol S, benzophenone-1 (BP-1), benzophenone-3 (BP-3), 4-hydroxybenzophenone, methylparaben (MeP), ethylparaben and propylparaben (PrP) were detected in more than 50% of the urine samples, with median concentrations of 0.938 ng/mL, 0.0111 ng/mL, 0.191 ng/mL, 1.30 ng/mL, 0.0320 ng/mL, 25.9 ng/mL, 4.31 ng/mL and 1.94 ng/mL, respectively. A significant positive correlation was observed between BP-1 and BP-3, as well as between MeP and PrP, indicating metabolic transformation and combined use, respectively. The concentrations of MeP and PrP in females were significantly higher than those in males, suggesting that females were exposed to more MeP and PrP than males. Urinary concentrations of BPA, BP-3, MeP and PrP could be influenced by age. Other demographic information, such as annual household income, education and occupation was not associated with the exposure level of the targeted phenols in adults. The estimated daily intakes of the analytes except BPA were all below their respective tolerable/acceptable daily intake levels. This study profiles the demographic differences in the exposure to environmental phenols in general populations from rural areas and provides information on risk assessments.


Assuntos
Exposição Ambiental , Parabenos , Adulto , Masculino , Feminino , Humanos , Parabenos/análise , Exposição Ambiental/análise , Compostos Benzidrílicos/análise , Fenóis/análise , Benzofenonas , China
12.
Drug Des Devel Ther ; 16: 3573-3588, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36248245

RESUMO

Data from globocan statistic in 2020 indicate that breast cancer has become highest incidence rate of cancer. Estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) are known immunohistochemistry (IHC) markers that mediate cell growth and survival signaling. Furthermore, regulator proteins, receptors, and their downstream signaling pathways have emerged as critical components in breast cancer formation and proliferation, and have become well-established therapeutic targets and the core focus of breast cancer therapy research. Garcinia is a big genus in the Clusiaceae family that contains a wide spectrum of biologically active metabolites for the chemical composition of their isolated fruits, stem barks, seeds, leaves, and roots, have resulted including polyisoprenylated benzophenones, polyphenols, bioflavonoids, xanthones, lactones, and triterpenes. This review article aimed to analyze the potential of Garcinia phytochemicals as a molecular therapy of breast cancer. The results showed that phytochemicals of Garcinia (i.e., α-mangostin, Cambogin, Gambogic Acid [GA], Garcinol, Griffipavixanthone, Friedolanostane triterpenoid, Hexane, Neobractatin, 7-Epiclusianone, xanthochymol - guttiferone E, and isoxanthochymol - cycloxanthochymol) have anticancer properties, including apoptosis, inhibition of proliferation, and metastasis. This review is important to provide information regarding phytochemicals of Garcinia as an alternative treatment for breast cancer patients. This article selected 28 article researches based on inclusion criteria with the keyword "Garcinia" and "Breast cancer", in English, and available in full text and abstract searching on PubMed.


Assuntos
Garcinia , Neoplasias , Plantas Medicinais , Triterpenos , Xantonas , Benzofenonas/química , Benzofenonas/farmacologia , Garcinia/química , Medicina Herbária , Hexanos , Humanos , Lactonas , Compostos Fitoquímicos/farmacologia , Polifenóis , Receptores de Estrogênio , Receptores de Progesterona , Triterpenos/química , Xantonas/química
13.
Inorg Chem ; 61(45): 18033-18043, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36315847

RESUMO

A novel mechanism of organobase-mediated Brook rearrangement and C-C coupling in the copper-catalyzed reductive coupling of benzaldehyde and benzophenone is proposed. The results demonstrate that this reaction proceeds mainly through five sequential elementary steps: transmetalation, carbonyl addition, σ-bond metathesis, Brook rearrangement, and C-C coupling. The organobases played a significant role not only in forming the active catalyst but also in mediating the Brook rearrangement and chemoselectivity in homo- and cross-coupling. Brook rearrangement mediated by organobases is more favored than that without organobases. In the C-C coupling step, the cation bridge combines two O atoms with the same electronegativity to form a pre-reaction complex. Moreover, a significant charge difference is a major factor in the selectivity of carbonyl addition and C-C coupling.


Assuntos
Benzaldeídos , Cobre , Catálise , Benzofenonas , Cátions
14.
Reprod Toxicol ; 114: 44-51, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36209923

RESUMO

It has been demonstrated that benzophenone-3 is one of the endocrine-disrupting compounds which are considered as potential risk factors of adverse health effects. However, whether benzophenone-3 exposure can influence the sex steroid hormones levels remains unknown. We used data from the National Health and Nutrition Examination Survey, which is a cross-sectional dataset, from 2013 to 2016. A total of 1690 male US participants aged 18 or above were included. Urinary benzophenone-3, serum total testosterone, serum estradiol, serum sex hormone-binding globulin were measured. Confounders including age, body mass index, race, education level, urinary creatinine, ratio of family income to poverty, alcohol use, time of venipuncture, cardiac arterial diabetic score, energy intake, bisphenol A, triclosan and total parabens were controlled. After full adjustment (Model III), the upper benzophenone-3 quintiles had odds ratios (95 % confidence intervals) of testosterone deficiency of 1.75 (1.03, 2.99), 2.47 (1.53, 3.98), 2.08 (1.13, 3.84) and 1.74 (0.94, 3.23) compared with quintile 1. Compared with quintile 1, percent changes (95 % confidence intervals) in testosterone were - 12 % (-19 %, -5 %) and - 9 % (-17 %, -1 %) for quintile 3 and quintile 5 in Model III. Estradiol and sex hormone-binding globulin were generally similar to total testosterone in the associations with benzophenone-3. In conclusion, our results demonstrated that adult men in the US with higher urinary benzophenone-3 had a higher risk of testosterone deficiency and had inverse associations with total testosterone, estradiol and sex hormone-binding globulin. To confirm the causal links between benzophenone-3 and sex steroid hormones, prospective studies are needed.


Assuntos
Benzofenonas , Disruptores Endócrinos , Hormônios Esteroides Gonadais , Globulina de Ligação a Hormônio Sexual , Adulto , Humanos , Masculino , Estudos Transversais , Estradiol , Inquéritos Nutricionais , Testosterona , Estados Unidos/epidemiologia , Disruptores Endócrinos/toxicidade , Benzofenonas/toxicidade
15.
Mediators Inflamm ; 2022: 8886087, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36081652

RESUMO

Background: Guttiferone E is a naturally occurring polyisoprenylated benzophenone exhibiting a wide range of remarkable biological activities. But its therapeutic application is still limited due to its poor water solubility. This study is aimed at preparing guttiferone E-loaded liposomes and assessing their in vitro cytotoxicity and anti-inflammatory effect. Methods: Liposomes containing guttiferone E were prepared by the thin film hydration method, and the physicochemical characteristics were determined using dynamic light scattering, laser Doppler velocimetry, and atomic force microscopy. The cytotoxicity was assessed by the MTT assay. The fluorometric cyclooxygenase (COX) activity assay kit was used to assess the COX activity while the nitric oxide production was evaluated by the Griess reagent method. Results: The liposomes with a mean size of 183.33 ± 17.28 nm were obtained with an entrapment efficiency of 63.86%. Guttiferone E-loaded liposomes successfully decreased the viability of cancer cells. The overall IC50 values varied between 5.46 µg/mL and 22.25 µg/mL. Compared to the untreated control, guttiferone E-loaded liposomes significantly reduced the nitric oxide production and the activity of COX in a concentration-dependent manner. Conclusion: This study indicates that liposomes can be an alternative to overcome the water insolubility issue of the bioactive guttiferone E.


Assuntos
Lipossomos , Neoplasias , Anti-Inflamatórios/farmacologia , Benzofenonas/farmacologia , Humanos , Lipopolissacarídeos/farmacologia , Macrófagos , Óxido Nítrico , Tamanho da Partícula , Água
16.
Int J Oral Implantol (Berl) ; 15(3): 277-286, 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36082661

RESUMO

PURPOSE: To prove that a fully digital workflow, even for registration of the maxillomandibular relationship, can be employed to produce implant-supported overdentures and demonstrate that CAD/CAM techniques can be used to mill permanent implant-supported overdentures from polymethylmethacrylate discs, using polyetheretherketone as the sliding mechanism. MATERIALS AND METHODS: An edentulous 64-year-old woman received six implants in the maxilla after a bone augmentation procedure and two implants in the mandible. Five months after implant placement, intraoral scans were taken of her original complete dentures, of each prosthesis individually, and of both in centric relation, and another was taken of both edentulous arches, including the scan bodies. Along with facial photographs, sufficient digital data were gathered to design and mill titanium bars, polyetheretherketone female parts and a trial implant-supported overdenture. The tooth positions and colour were discussed with the patient using Smile Design software (3Shape, Copenhagen, Denmark). In the second session, the bars and trial implant-supported overdenture were inserted and checked, and in the third session, the final implant-supported overdentures were inserted. RESULTS: The milled titanium bar exhibited a passive fit, as did the implant-supported overdentures. After 1 year, no prosthetic complications were noted; the measured pockets were all less than 4 mm in depth. On a visual analogue scale from 0 to 10, with 0 being the worst and 10 being the best, the patient awarded a score of 9 for her satisfaction with the implant-supported overdentures. CONCLUSION: A fully digital workflow enables the production of robust wear-resistant implant-supported overdentures milled from polymethylmethacrylate, using polyetheretherketone female parts as the sliding mechanism, in just three clinical sessions.


Assuntos
Implantes Dentários , Arcada Edêntula , Benzofenonas , Prótese Dentária Fixada por Implante , Revestimento de Dentadura , Feminino , Humanos , Cetonas , Pessoa de Meia-Idade , Polímeros , Polimetil Metacrilato , Titânio , Fluxo de Trabalho
17.
J Mater Sci Mater Med ; 33(10): 68, 2022 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-36178551

RESUMO

Percutaneous implants are frequently affected by bacterial growth at the skin-implant interface. Integration between implant and surrounding skin is important to prevent bacteria from spreading to the underlying tissue. The standard method to evaluate skin-implant integration is by histomorphometry on samples which have been placed in tissue grown in vivo or ex vivo. In this study, a biomechanical method was developed and evaluated. The integration of implants into porcine skin was studied in an ex vivo model, where pig skin samples were cultivated in a nutrient solution. Cylindrical shaped implants, consisting of polyether ether ketone (PEEK) and titanium (Ti) with different surface treatments, were implanted in the skin tissue and the skin was grown in nutrient solution for 2 weeks. The implants were then extracted from the implantation site and the mechanical force during extraction was measured as a quantitative assessment of skin-implant integration. Implants from each group were also processed for histomorphometry and the degree of epidermal downgrowth (ED) and tissue to implant contact (TIC) was measured. A higher mean pullout force was observed for the PEEK implants compared to the Ti implants. Applying nanosized hydroxyapatite (HA) on Ti and PEEK increased the pullout force compared to uncoated controls, 24% for machined and 70% for blasted Ti, and 51% for machined PEEK. Treatment of Ti and PEEK with nanosized zirconium phosphate (ZrP) did not increase the pullout force. The histomorphometry analysis showed correlation between ED and pullout force, where the pullout force was inversely proportional to ED. For TIC, no significant differences were observed between the groups of same material (i.e. Ti, Ti+HA, Ti+ZrP, and PEEK, PEEK + HA, PEEK + ZrP), but it was significantly higher for PEEK compared to Ti. Scanning electron microscopy analysis was done on samples before and after the pullout tests, showing that the ZrP coating was unaffected by the 2 week ex vivo implantation and pullout procedure, no dissolution or detachment of the coating was observed. For the HA coating, a loss of coating was seen on approximately 5% of the total surface area of the implant. Graphical abstract.


Assuntos
Implantes Dentários , Titânio , Animais , Benzofenonas , Durapatita , Éteres , Cetonas , Osseointegração , Polietilenoglicóis , Polímeros , Propriedades de Superfície , Suínos
18.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 36(9): 1126-1131, 2022 Sep 15.
Artigo em Chinês | MEDLINE | ID: mdl-36111476

RESUMO

Objective: To compare the effectiveness between three-dimensional (3D) printed porous titanium alloy cage (3D Cage) and poly-ether-ether-ketone cage (PEEK Cage) in the posterior lumbar interbody fusion (PLIF). Methods: A total of 66 patients who were scheduled to undergo PLIF between January 2018 and June 2019 were selected as the research subjects, and were divided into the trial group (implantation of 3D Cage, n=33) and the control group (implantation of PEEK Cage, n=33) according to the random number table method. Among them, 1 case in the trial group did not complete the follow-up exclusion study, and finally 32 cases in the trial group and 33 cases in the control group were included in the statistical analysis. There was no significant difference in gender, age, etiology, disease duration, surgical segment, and preoperative Japanese Orthopaedic Association (JOA) score between the two groups (P>0.05). The operation time, intraoperative blood loss, complications, JOA score, intervertebral height loss, and interbody fusion were recorded and compared between the two groups. Results: The operations of two groups were completed successfully. There was 1 case of dural rupture complicated with cerebrospinal fluid leakage during operation in the trial group, and no complication occurred in the other patients of the two groups. All incisions healed by first intention. There was no significant difference in operation time and intraoperative blood loss between groups (P>0.05). All patients were followed up 12-24 months (mean, 16.7 months). The JOA scores at 1 year after operation in both groups significantly improved when compared with those before operation (P<0.05); there was no significant difference between groups (P>0.05) in the difference between pre- and post-operation and the improvement rate of JOA score at 1 year after operation. X-ray film reexamination showed that there was no screw loosening, screw rod fracture, Cage collapse, or immune rejection in the two groups during follow-up. At 3 months and 1 year after operation, the rate of intervertebral height loss was significantly lower in the trial group than in the control group (P<0.05). At 3 and 6 months after operation, the interbody fusion rating of trial group was significantly better in the trial group than in the control group (P<0.05); and at 1 year after operation, there was no significant difference between groups (P>0.05). Conclusion: There is no significant difference between 3D Cage and PEEK Cage in PLIF, in terms of operation time, intraoperative blood loss, complications, postoperative neurological recovery, and final intervertebral fusion. But the former can effectively reduce vertebral body subsidence and accelerate intervertebral fusion.


Assuntos
Cetonas , Fusão Vertebral , Ligas , Benzofenonas , Perda Sanguínea Cirúrgica , Parafusos Ósseos , Éter , Éteres , Etil-Éteres , Humanos , Vértebras Lombares/cirurgia , Polímeros , Porosidade , Fusão Vertebral/métodos , Titânio , Resultado do Tratamento
19.
Sci Rep ; 12(1): 15816, 2022 09 22.
Artigo em Inglês | MEDLINE | ID: mdl-36138117

RESUMO

Radiolucent carbon-fiber reinforced PEEK (CFRP) implants have helped improve oncological follow-up and radiation therapy. Here, we investigated the performance of 3D intraoperative imaging and navigation systems for instrumentation and precision assessment of CFRP pedicle screws across the thoraco-lumbar spine. Thirty-three patients with spinal tumors underwent navigated CFRP instrumentation with intraoperative CT (iCT), robotic cone-beam CT (rCBCT) or cone-beam CT (CBCT) imaging. Two different navigation systems were used for iCT-/rCBCT- and CBCT-based navigation. Demographic, clinical and outcome data was assessed. Four blinded observers rated image quality, assessability and accuracy of CFRP pedicle screws. Inter-observer reliability was determined with Fleiss` Kappa analysis. Between 2018 and 2021, 243 CFRP screws were implanted (iCT:93, rCBCT: 99, CBCT: 51), of which 13 were non-assessable (iCT: 1, rCBCT: 9, CBCT: 3; *p = 0.0475; iCT vs. rCBCT). Navigation accuracy was highest using iCT (74%), followed by rCBCT (69%) and CBCT (49%) (*p = 0.0064; iCT vs. CBCT and rCBCT vs. CBCT). All observers rated iCT image quality higher than rCBCT/CBCT image quality (*p < 0.01) but relevant pedicle breaches were reliably identified with substantial agreement between all observers regardless of the imaging modality. Navigation accuracy for CFRP pedicle screws was considerably lower than expected from reports on titanium implants and CT may be best for reliable assessment of CFRP materials.


Assuntos
Fusão Vertebral , Cirurgia Assistida por Computador , Benzofenonas , Fibra de Carbono , Humanos , Cetonas , Plásticos , Polietilenoglicóis , Polímeros , Reprodutibilidade dos Testes , Estudos Retrospectivos , Fusão Vertebral/métodos , Cirurgia Assistida por Computador/métodos , Titânio , Tomografia Computadorizada por Raios X/métodos
20.
Aquat Toxicol ; 251: 106285, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36087491

RESUMO

The health concern associated with the dangers related to exposure to UV radiation has led to an increase in the use of sunscreens containing UV-filters that can reach aquatic environments and possibly affect ecosystems. Benzophenone-3 (BP-3) and benzophenone-4 (BP-4) are two of the most used UV-filters. In the present work, the microalga Chlamydomonas reinhardtii was exposed to several concentrations of both chemicals. To evaluate their potential cytotoxicity on microalgal cells, different parameters were analysed including fast response biomarkers (increase in intracellular free Ca2+) as well as biomarkers related with the presence of oxidative stress (lipid peroxidation), energy metabolism (photosynthetic yield and cytoplasmic lipid accumulations), cell division (proliferation and F-actin content), programmed cell death (PCD) (caspase activation and DNA fragmentation) and possible mechanisms of resistance to xenobiotics (operation of extrusion pumps and presence of autophagic vacuoles). Results showed an increment of the percentage of cells with cytosolic free Ca2+ that could act as a secondary messenger in response to the stress. A decrease in photosynthetic yield and an increase in cytoplasmic lipid accumulations and lipid peroxidation levels were also detected. In addition, a decrease in cell proliferation was observed, linked to a decrease in the percentage of cells with F-actin. The increase observed in the microalgal population with caspase activity, together with the DNA fragmentation and the alterations in the cytoskeleton, suggested the induction of processes linked to PCD. Moreover, a blockage of extrusion pumps, which could be related to the toxicity mechanism of these compounds, and an increase in autophagic vacuoles, as an attempt to repair the damage caused by benzophenones, were detected. Overall, these biomarkers indicate that both UV-filters can be a serious threat to non-target photosynthetic microorganisms in aquatic environments, although BP-3 affected C. reinhardtii more markedly.


Assuntos
Chlamydomonas reinhardtii , Poluentes Químicos da Água , Actinas/metabolismo , Apoptose , Benzofenonas/toxicidade , Biomarcadores/metabolismo , Caspases/metabolismo , Chlamydomonas reinhardtii/metabolismo , Ecossistema , Lipídeos , Estresse Oxidativo , Protetores Solares/toxicidade , Poluentes Químicos da Água/toxicidade
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