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1.
Zhongguo Zhong Yao Za Zhi ; 46(15): 3893-3899, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34472265

RESUMO

To explore the protective effect and mechanism of ethyl acetate extract from Bidens bipinnata on hepatocyte damage induced by endoplasmic reticulum stress. Tunicamycin was used to establish the damage model in L02 cells. Methyl thiazolyl tetrazolium(MTT) colorimetric assay was used to investigate the survival rate of ethyl acetate extract from B. bipinnata in L02 cells injury induced by endoplasmic reticulum stress; the protein expressions of endoplasmic reticulum stress-related molecule glucose regulated protein 78(GRP78), PKR-like ER kinase(PERK), eukaryotic initiation factor-2(eIF2α), activating transcription factor 4(ATF4), C/EBP homologous protein(CHOP), B-cell CLL/lymphoma 2(Bcl-2), Bal-2 associated X apoptosis regulator(Bax) were examined by Wes-tern blot. The expressions of the above proteins were also detected after endoplasmic reticulum stress inhibitor(4-phenyl butyric acid) and CHOP shRNA-mediated knockdowns were added. The expressions of GRP78, PERK, CHOP in L02 cells were observed by immunofluorescence method. The results showed that ethyl acetate extract from B. bipinnata could significantly increase the survival rate of L02 cell injury caused by endoplasmic reticulum stress in a dose and time-dependent manner(P<0.05 or P<0.01). The expression levels of GRP78, PERK, eIF2α, ATF4, CHOP and Bax in the drug treatment groups were significantly down-regulated(P<0.05 or P<0.01), while Bcl-2 was significantly up-regulated(P<0.01). After endoplasmic reticulum stress inhibitor and CHOP shRNA-mediated knockdowns were added, the expression levels of GRP78, PERK, eIF2α, ATF4, CHOP, Bax in the drug treatment groups were significantly down-regulated(P<0.01), whereas Bcl-2 was significantly up-regulated(P<0.01). Immunofluorescence results showed that the expressions of GRP78, PERK, CHOP were consistent with the Western blot method. In conclusion, ethyl acetate extract from B. bipinnata has a significant protective effect on the damage of L02 cells caused by endoplasmic reticulum stress. The mechanism may be related to the inhibition of endoplasmic reticulum stress and the down-regulation of apoptosis in cells through the PERK/eIF2α/ATF4/CHOP signaling pathway.


Assuntos
Bidens , Estresse do Retículo Endoplasmático , Acetatos , Apoptose , Hepatócitos , Fator de Transcrição CHOP/genética , eIF-2 Quinase/genética
2.
Braz Oral Res ; 35: e063, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34076188

RESUMO

FITOPROT, which contains curcuminoids and Bidens pilosa L. extract, is an innovative mucoadhesive formulation indicated for the topical treatment of chemoradiotherapy-induced oral mucositis (OM) in patients with advanced and visible oral squamous cell carcinoma. The formulation is used as a mouthwash directly on tumor tissue of patients with advanced neoplasms, without triggering cancer cell proliferation or tumor invasiveness. Thus, the aim of this study was to evaluate the biological effects of FITOPROT on an oral squamous cell carcinoma cell line (SCC-4). The viability of SCC-4 cells was assessed after exposure to FITOPROT using MTT reduction assay. The effects of the mucoadhesive formulation on cell cycle progression and cell death parameters were evaluated using flow cytometry. In addition, the inflammatory profile of the tumor cells was evaluated using the cytometric bead array (CBA) assay. FITOPROT promoted a concentration-dependent decrease in cell viability and cell cycle arrest at the G2/M phase (p < 0.05). Mitochondrial membrane potential was also altered after exposure to the formulation (p < 0.05), in parallel with a reduction in VEGF and IL-8 production (p = 0.01 and p = 0.05, respectively). In summary, the results indicate that FITOPROT reduces SCC-4 cell viability, promotes cell cycle arrest, modulates mitochondrial membrane potential, and exhibits antiangiogenic and anti-inflammatory properties, thus indicating its potential for topical use in patients with OM and visible tumors in the mouth.


Assuntos
Bidens , Carcinoma de Células Escamosas , Neoplasias Bucais , Apoptose , Carcinoma de Células Escamosas/tratamento farmacológico , Linhagem Celular , Proliferação de Células , Diarileptanoides , Humanos , Neoplasias Bucais/tratamento farmacológico
3.
Ecotoxicol Environ Saf ; 219: 112332, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34044313

RESUMO

Cadmium (Cd) due to its strong toxicity and high mobility, which poses a considerable threat to soil environment and human health, has aroused widespread concern. Biochar has been used for remediating Cd-contaminated soil recently, however this method has the risk of fixed-Cd re-release. Phytoremediation can make up for its shortcoming. In this study, a pot experiment was carried out, where Bidens pilosa L. (B.pilosa) was as the tested plant and biochars (maize straw biochar and wheat straw biochar with two particle sizes) were as amendments. The mechanism of how biochars promoted B.pilosa Cd accumulation in Cd-contaminated farmland soil was explored. Results showed that the application of 5% wheat straw fine biochar (WF), wheat straw coarse biochar (WC), maize straw fine biochar (MF) and maize straw coarse biochar (MC) increased the total Cd accumulation of B.pilosa to 251.57%, 217.41%, 321.64% and 349.66%, respectively. Biochars amendment significantly promoted B.pilosa growth and increased Cd accumulation by improving soil physical properties, nutrient levels (available nitrogen, available phosphorus (AP), available potassium (AK) and organic matter (OM)) and microbial activity, and changing the nutrients distribution in B.pilosa organs although tissues although DTPA-Cd reduced to some extent. The effect of MF on AP increase was better than MC, while the effect of WF on AK increase was better than WC. Fine-particle was superior to coarse-particle in increasing B.pilosa biomass of aboveground, OM and microbial activity in soil. The changes of N, P and K concentrations in B.pilsosa roots, stems and leaves were closely related to the changes of AN, AP and AK in soil after biochars application. The results indicated that the combination of straw biochars and hyperaccumulators had the synergistic effect. This study can provide data support and meaningful reference values for remediating actual Cd-contaminated soil.


Assuntos
Bidens/fisiologia , Biodegradação Ambiental , Cádmio/análise , Poluentes do Solo/análise , Biomassa , Carvão Vegetal , Fazendas , Humanos , Nitrogênio , Fósforo , Raízes de Plantas/química , Solo , Triticum , Zea mays
4.
J Ethnopharmacol ; 275: 114097, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33823164

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: As a traditional Chinese medicinal, Bidens bipinnata L. has been used to treat many diseases with a long history in China. The anti-diabetic effects of extract from B. bipinnata have been demonstrated in the previous reports. AIM OF THE STUDY: The protective effects of flavonoids-rich extract from B. bipinnata (BBTF) on cell damage induced by H2O2 in pancreatic ß cell and its potential mechanisms were evaluated. MATERIALS AND METHODS: MTT, ROS production, nuclear staining and flow cytometry assays were adopted to determine the effects of BBTF on cell viability, production of ROS and cell apoptosis in H2O2-treated INS-1 cell. Cell apoptosis-related proteins expressions were detected by Western blot assay. RESULTS: Pre-treatment of BBTF could significantly increase INS-1 cell viability, inhibit the production of intracellular ROS and reduced the characteristic features of cell apoptosis induced by H2O2 in INS-1 cells. The studies of the underlying mechanism showed that BBTF could regulate Bax and Bcl-2 proteins expressions, suppress the phosphorylation of JNK, ERK and p38, as well as down-regulate Fas and FasL proteins expressions induced by H2O2. The expressions of caspase-8, caspase-9 and caspase-3 were therefore decreased. CONCLUSION: The results indicated that flavonoids-rich extract from B. bipinnata could be a natural agent in diabetic prevention and therapy.


Assuntos
Apoptose/efeitos dos fármacos , Bidens/química , Medicamentos de Ervas Chinesas/farmacologia , Flavonoides/farmacologia , Células Secretoras de Insulina/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Animais , Caspases/metabolismo , Linhagem Celular Transformada , Sobrevivência Celular/efeitos dos fármacos , Medicamentos de Ervas Chinesas/isolamento & purificação , Proteína Ligante Fas/metabolismo , Flavonoides/isolamento & purificação , Peróxido de Hidrogênio/toxicidade , Células Secretoras de Insulina/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Substâncias Protetoras/isolamento & purificação , Substâncias Protetoras/farmacologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Espécies Reativas de Oxigênio/metabolismo , Proteína X Associada a bcl-2/metabolismo , Receptor fas/metabolismo
5.
Molecules ; 26(7)2021 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-33808049

RESUMO

Cyclic imides belong to a well-known class of organic compounds with various biological activities, promoting a great interest in compounds with this functional group. Due to the structural complexity of some molecules and their spectra, it is necessary to use several spectrometric methods associated with auxiliary tools, such as the theoretical calculation for the structural elucidation of complex structures. In this work, the synthesis of epoxy derivatives of 5-methylhexahydroisoindole-1,3-diones was carried out in five steps. Diels-Alder reaction of isoprene and maleic anhydride followed by reaction with m-anisidine afforded the amide (2). Esterification of amide (2) with methanol in the presence of sulfuric acid provided the ester (3) that cyclized in situ to give imides 4 and 4-ent. Epoxidation of 4 and 4-ent with meta-chloroperbenzoic acid (MCPBA) afforded 5a and 5b. The diastereomers were separated by silica gel flash column chromatography, and their structures were determined by analyses of the spectrometric methods. Their structures were confirmed by matching the calculated 1H and 13C NMR chemical shifts of (5a and 5b) with the experimental data of the diastereomers using MAE, CP3, and DP4 statistical analyses. Biological assays were carried out to evaluate the potential herbicide activity of the imides. Compounds 5a and 5b inhibited root growth of the weed Bidens pilosa by more than 70% at all the concentrations evaluated.


Assuntos
Compostos de Epóxi , Herbicidas , Imidas , Sementes/crescimento & desenvolvimento , Bidens/crescimento & desenvolvimento , Cucumis sativus/crescimento & desenvolvimento , Compostos de Epóxi/síntese química , Compostos de Epóxi/química , Herbicidas/síntese química , Herbicidas/química , Imidas/síntese química , Imidas/química , Alface/crescimento & desenvolvimento , Estrutura Molecular , Sorghum/crescimento & desenvolvimento
6.
Planta Med ; 87(7): 550-559, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33572002

RESUMO

Rheumatoid arthritis is a chronic autoimmune disease characterized by the infiltration of synovial inflammatory cells and progressive joint destruction. Total flavonoids of Bidens pilosa have been used against inflammation in rheumatoid arthritis, but its role in bone destruction remains to be explored. The aim of this paper was to study whether total flavonoids of B. pilosa relieve the severity of collagen-induced arthritis in rats, particularly whether it regulates the production of proinflammatory cytokines and the receptor activator of nuclear factor-κB/receptor activator of nuclear factor-κB ligand/osteoprotegerin signaling pathway. In this research, a collagen-induced disease model was induced in adult rats by subcutaneous injection of collagen II. Total flavonoids of B. pilosa at different doses (40, 80, and 160 mg/kg/d) were administered intragastrically, while methotrexate (1 mg/kg/w) was injected intraperitoneally as a positive control. Paw swelling, arthritis score, and body weight were assessed and evaluated. The severity of joint damage was determined using X-ray and confirmed by histopathology. The expression levels of receptor activator of nuclear factor-κB ligand, osteoprotegerin, IL-1ß, IL-17, and TNF in the serum and tissue were assayed using ELISA and immunohistochemistry. We found that total flavonoids of B. pilosa attenuated collagen-induced arthritis at the macroscopic level, and total flavonoids of B. pilosa-treated rats showed reduced paw swelling, arthritis scores, and X-ray appearance of collagen-induced arthritis in addition to improved histopathological results. These findings were consistent with reduced serum and tissue receptor activator of nuclear factor-κB ligand, TNF, IL-1ß, and IL-17 levels but increased osteoprotegerin levels. Our data suggest that total flavonoids of B. pilosa attenuate collagen-induced arthritis by suppressing the receptor activator of nuclear factor-κB ligand/receptor activator of nuclear factor-κB/osteoprotegerin pathway and the subsequent production of proinflammatory cytokines. In addition, total flavonoids of B. pilosa may be a promising therapeutic candidate for the management of rheumatoid arthritis.


Assuntos
Artrite Experimental , Artrite Reumatoide , Bidens , Animais , Artrite Experimental/tratamento farmacológico , Artrite Reumatoide/tratamento farmacológico , Citocinas , Flavonoides/farmacologia , NF-kappa B , Ratos
7.
Molecules ; 26(2)2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33419109

RESUMO

Bidens pilosa L. (Asteraceae) has been used historically in traditional Asian medicine and is known to have a variety of biological effects. However, the specific active compounds responsible for the individual pharmacological effects of Bidens pilosa L. (B. pilosa) extract have not yet been made clear. This study aimed to investigate the anti-inflammatory phytochemicals obtained from B. pilosa. We isolated a flavonoids-type phytochemical, isookanin, from B. pilosa through bioassay-guided fractionation based on its capacity to inhibit inflammation. Some of isookanin's biological properties have been reported; however, the anti-inflammatory mechanism of isookanin has not yet been studied. In the present study, we evaluated the anti-inflammatory activities of isookanin using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We have shown that isookanin reduces the production of proinflammatory mediators (nitric oxide, prostaglandin E2) by inhibiting the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated macrophages. Isookanin also inhibited the expression of activator protein 1 (AP-1) and downregulated the LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-jun NH2-terminal kinase (JNK) in the MAPK signaling pathway. Additionally, isookanin inhibited proinflammatory cytokines (tumor necrosis factor-a (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-1ß (IL-1ß)) in LPS-induced THP-1 cells. These results demonstrate that isookanin could be a potential therapeutic candidate for inflammatory disease.


Assuntos
Anti-Inflamatórios , Bidens/química , Bioensaio , Chalconas , Macrófagos/metabolismo , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Chalconas/química , Chalconas/isolamento & purificação , Chalconas/farmacologia , Humanos , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Lipopolissacarídeos/toxicidade , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Macrófagos/patologia , Camundongos , Monocinas/metabolismo , Células RAW 264.7 , Células THP-1
8.
Chemosphere ; 273: 129652, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33515966

RESUMO

Heavy metal concentrations accumulated by different ecotypes of the same hyperaccumulator, collected from contaminated and uncontaminated areas, were found to vary significantly. Very few studies have compared the accumulative properties of two ecotype hyperaccumulators originating from clean soils. Here we compared the Hanzhong ecotype of Bidens pilosa L. (HAE), originating from clean soil in a subtropical monsoon climate zone Hanzhong city, with the Shenyang ecotype (SHE), originating from clean soil in a temperate semi humid continental climate zone Shenyang city, and we universally observed higher Cd concentration and higher biomass in the HAE ecotype. Both HAE and SHE demonstrated similar general Cd hyperaccumulator properties in S1 soil (4.43 mg kg-1 Cd) and S2 soil (49.79 mg kg-1 Cd, but HAE exhibited a higher net photosynthetic rate, transpiration rate, SOD activity and greater extractable Cd concentration in its rhizospheric soil. These results might imply that some ecotypes of hyperaccumulator in different climate zone may show higher phytoextraction potential. The differences of Cd accumulation among ecotypes may be more useful for the identification of genes relevant to plant hyperaccumulation.


Assuntos
Bidens , Poluentes do Solo , Biodegradação Ambiental , Cádmio/análise , Ecótipo , Solo , Poluentes do Solo/análise
9.
Ecotoxicol Environ Saf ; 209: 111847, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33388723

RESUMO

Bidens pilosa L. is a widely distributed Cd-hyperaccumulator species in the world with large biomass and fast growth rate. The Cd accumulating differences between different ecotypes of B. pilosa is not clear. This experiment firstly compared the Cd concentrations and relative physio-biochemical indices using two B. pilosa ecotypes collected from clean soils. The results showed that the Cd concentrations of stems and leaves of Hanzhong ecotype of B. pilosa (HZ) and Shenyang ecotype (SY) were all higher than their root Cd concentrations in different Cd concentration gradient experiment (from 2.57 mg kg-1 to 37.17 mg kg-1 in soils). Cd concentrations of the roots, stems and leaves of HZ and SY were all higher than in the soils either. However, HZ accumulated higher Cd concentrations than SY, i.e. roots increased by 32.7-45.8%, stems increased by 32.3-46.6% and leaves increased by 33.4-68.4%, respectively. Furthermore, the biomasses of HZ were all higher than the SY either. Compared to SY, higher Cd accumulation of HZ might be relevant with its higher photosynthetic pigment content, stomatal conductance, intercellular CO2 concentration, some antioxidant enzyme activities, H+-ATPase, Ca2+-ATPase and 5'-AMPase activities, and lower malondialdehyde (MDA) content. Particularly, the changes of extractable Cd concentrations in rhizospheric soils of HZ and SY were corresponding to their Cd concentrations. Considering the two different ecotypes of HZ and SY were all collected from different clean farmlands, the new foundings that different mechanisms of HZ and SY accumulating Cd from the soil might be very important for screening and constructing ideal hyperaccumulator aimed at improving phytoremediation capacities in the future.


Assuntos
Bidens/fisiologia , Cádmio/metabolismo , Poluentes do Solo/metabolismo , Biodegradação Ambiental , Biomassa , Cádmio/análise , Ecótipo , Fazendas , Malondialdeído , Folhas de Planta/química , Raízes de Plantas/química , Solo , Poluentes do Solo/análise
10.
Chemosphere ; 269: 128732, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33143889

RESUMO

Phytoremediation, especially phytoextraction, is a good alternative for remediation of soils contaminated with heavy metals. This method requires selection of species for their tolerance, high accumulation levels in harvestable parts, and high biomass production. Bidens pilosa L. has been reported as tolerant to and potentially hyperaccumulator of several heavy metals, including Pb, but with variable results in terms of effectiveness. The aim of this study was to analyse the intra- and interpopulation variability of B. pilosa in response to Pb in individuals from two populations: one historically exposed to Pb and another with no history of exposure. Bidens pilosa L. presented tolerance to Pb pollution in soil, evidenced in a higher survival rate, a better antioxidant response, and an efficient reduction in cell membrane damage mainly due to history of exposure. The period of exposure (30 years) was not long enough to obtain a B. pilosa population that provides seeds for phytoextraction projects, since the average value of total extraction was relatively low. Collecting seeds from a historically exposed population will provide some suitable individuals with Pb accumulation and translocation capabilities, but not a sufficient amount to conduct a large phytoremediation project. The individual accumulator profile of B. pilosa is not related to the physiological behaviour or to the Pb entry into the vascular bundle in root, but to the incorporation of other heavy metals that are micronutrients.


Assuntos
Bidens , Metais Pesados , Poluentes do Solo , Biodegradação Ambiental , Humanos , Chumbo , Solo , Poluentes do Solo/análise
11.
Biomed Chromatogr ; 35(4): e5026, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33169423

RESUMO

Bidens bipinnata L. is a folk medicinal plant in China that shows significant antihyperlipidemia effectiveness. However, studies of the underlying mechanism study are lacking. In order to explore the potential action sites and the underlying mechanism of treating hyperlipidemic, this work undertook tissue distribution and molecular docking research on the markers of B. bipinnata L., which were obtained through serum pharmacochemistry and network database retrieval. The results showed that seven compounds (gallic acid, protocatechuic acid, rutin, hyperoside, bipinnate polyacetylenicloside, luteolin and quercetin) were screened out as markers. Owing to the diversity of chemical structures, they exhibited an inconsistent trend in tissue distribution. However, all of them had high levels in the liver and no specific distribution in other tissues. More interestingly, seven proteins-HMGCR (1HWK), NR3C1 (4P6W), CYP1A2 (2HI4), RXRA (4PP3), CES1 (1MX1), HSD11B1 (2RBE) and CYP1A1 (4I8V)-showed significant binding affinity with three or more markers, suggesting that they may be the target proteins of B. bipinnata L. This study preliminarily sheds light on the tissue distribution and targets of B. bipinnata L., providing some useful information on the underlying mechanisms of the antihyperlipidemia effect.


Assuntos
Bidens/química , Medicamentos de Ervas Chinesas , Hiperlipidemias/metabolismo , Animais , Catequina/análise , Catequina/farmacocinética , Cromatografia Líquida de Alta Pressão , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/farmacocinética , Ácido Gálico/análise , Ácido Gálico/farmacocinética , Modelos Lineares , Fígado/química , Fígado/metabolismo , Simulação de Acoplamento Molecular , Ratos , Reprodutibilidade dos Testes , Rutina/análise , Rutina/farmacocinética , Sensibilidade e Especificidade , Espectrometria de Massas em Tandem , Distribuição Tecidual
12.
J Exp Bot ; 72(2): 525-541, 2021 02 02.
Artigo em Inglês | MEDLINE | ID: mdl-33063830

RESUMO

Polyacetylene compounds from Bidens pilosa are known to have several pharmacological activities. In this study, we identified major genes encoding enzymes involved in the biosynthesis of polyacetylene in B. pilosa. Seven polyacetylene metabolites present in B. pilosa leaves were induced by methyl jasmonate (MeJA) treatment and physical wounding. Transcriptome analysis via high-throughput sequencing revealed 39 202 annotated gene fragment sequences. A DNA microarray established by the 39 202 annotated genes was used to profile gene expression in B. pilosa leaf and root tissues. As no polyacetylene compounds were found in roots, the gene expression pattern in root tissue was used as a negative control. By subtracting MeJA-induced genes in roots, we obtained 1216 genes in leaves showing an approximate three-fold increase in expression post-MeJA treatment. Nine genes encoding enzymes with desaturation function were selected for confirmation of expression by qRT-PCR. Among them, two genes, BPTC030748 and BPTC012564, were predicted to encode Δ12-oleate desaturase (OD) and Δ12-fatty acid acetylenase (FAA), respectively. In B. pilosa leaves, RNAi knock-down concomitantly decreased, while virus-mediated transient overexpression of either gene elevated polyacetylene content. In summary, we demonstrate that two important enzymes, Δ12-oleate desaturase and Δ12-fatty acid acetylenase, involved in desaturation of linear fatty acid precursors play a role in polyacetylene biosynthesis in an important medicinal plant, Bidens pilosa.


Assuntos
Bidens , Plantas Medicinais , Bidens/genética , Vias Biossintéticas , Folhas de Planta , Polímero Poliacetilênico
13.
J Environ Manage ; 280: 111746, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33298398

RESUMO

Exotic invasive plants endanger the integrity of agricultural and natural systems throughout the world. Thus, the development of cost-effective and economic application of invasive plants is warranted. Here, we characterized fifteen biochars derived from five invasive plants at different temperatures (300, 500, and 700 °C) by determining their yield, ash content, pH, CEC, surface area, elementary composition, functional groups, and mineral composition. We conducted batch adsorption experiments to investigate the adsorption capacity and efficiency for Cd2+ and Cu2+ in wastewater. Our results suggest that all invasive plants are appropriate for biochar production, temperature and plant species had interacting effects on biochar properties, and the biochars pyrolyzed at 500 and 700 °C exhibited high metal adsorption capacity in neutral (pH = 7) solutions. The adsorption kinetics can be explained adequately by a pseudo-second-order model. BBC500 (Bidens pilosa L. derived biochar at 500 °C) and MBC500 (Mikania micrantha) exhibited higher metal equilibrium adsorption capacities (38.10 and 38.02 mg g-1 for Cd2+, 20.01 and 20.10 mg g-1 for Cu2+) and buffer abilities to pH than other biochars pyrolyzed at 500 °C. The Langmuir model was a better fit for IBC500 (Ipomoea cairica), MBC500, and LBC500 (Lantana camara L.) compared to the Freundlich model, whereas the opposite was true for BBC500 and PBC500 (Praxelis clematidea). These results suggest that the adsorption of metals by IBC500, MBC500, and LBC500 was mainly monolayer adsorption, while that by BBC500 and PBC500 was mainly chemical adsorption. Our results are important for the utilization and control of invasive plants as well as the decontamination of aqueous pollution.


Assuntos
Asteraceae , Bidens , Ipomoea , Lantana , Mikania , Poluentes Químicos da Água , Adsorção , Cádmio/análise , Carvão Vegetal , Espécies Introduzidas , Poluentes Químicos da Água/análise
14.
São Paulo; s.n; 2021. 31 p. ilus.
Tese em Português | LILACS, Coleciona SUS, Sec. Est. Saúde SP, SESSP-SUCENPROD, Sec. Est. Saúde SP, SESSP-ESPECIALIZACAOSESPROD, Sec. Est. Saúde SP | ID: biblio-1179000

RESUMO

Os mosquitos da ordem Diptera e família Culicidae, configuram um grupo expressivo dentre os insetos. O gênero Culex é composto por 768 espécies divididas em 26 subgêneros. A estratificação vertical das espécies do gênero Culex varia de acordo com as tendências alimentares e sua oferta, entretanto, as fêmeas necessitam de aporte sanguíneo para a maturação dos ovos. Essa hematofagia faz com que os culicídeos sejam importantes vetores de arboviroses e outras doenças transmitidas pela picada destes insetos. Estudos demonstram que o gênero Culex é de extrema importância epidemiológica, sendo necessária a identificação correta dos insetos em regiões endêmicas como uma ferramenta vital de vigilância. A Morfometria Geométrica Alar (WGM) entra como um reforço, ou mesmo como alternativa, uma vez que tem obtido êxito em identificar corretamente espécies de mosquitos, provando ser uma técnica tão eficiente quanto a taxonomia clássica e a biologia molecular. A Simetria Bilateral usando WGM nos fornece a Assimetria Flutuante como um importante dado derivado, que pode ser definida por pequenos desvios de uma simetria bilateral perfeita para uma determinada característica morfológica. Neste estudo foram utilizadas asas de mosquitos culicídeos do gênero Culex, subgênero Culex previamente armazenadas de coletas realizadas na Fundação Parque Zoológico de São Paulo, Parques Municipais de São Paulo no Brasil e Patagônia, Argentina, entre os anos de 2015 a 2020. Após a identificação taxonômica, ambas as asas foram coletadas e posteriormente fixadas em lâmina e lamínula com Bálsamo do Canadá. Imagens foram capturadas através de lupa estereoscópica Leica com câmera acoplada. Nos resultados observamos que Culex habilitator e Culex coronator apresentaram maior simetria entre as duas asas, enquanto as asas de Culex acharistus e Culex habilitator apresentaram assimetrias mais acentuadas. A árvore fenética agrupou os pares de asas como o esperado, com excessão das asas de Culex habilitator coletadas no ano de 2015. Comparativamente, os Culex habilitator coletados em 2020 apresentaram mais simetria alar do que os coletados em 2015, e valores de reclassificação superiores. Esses resultados indicam que a técnica de WGM aplicada a simetria bilateral se mostra uma ferramenta versátil, permitindo a realização de diferentes testes estatísticos e morfológicos baseado nos mesmos 18 pontos já utilizados para identificação de mosquitos, porém evidenciando de forma mais sensível a influência sofrida por cada população amostrada pelo seu ambiente. Tendo as amostras de Culex bidens e Culex coronator como referências de Assimetria Flutuante mais próximas do ideal, observamos nas amostragens de Culex acharistus variações importantes, porem esperadas, por se tratar de uma população coletada em ambiente menos favorável para o desenvolvimento do mosquito. Também observamos variações importantes dentro das amostragens de Culex habilitator, que podem ter sido causadas por erro de identificação taxonômica ou ação de herbicida, porém essas duas possibilidades precisam ser melhor investigadas.


Assuntos
Classificação , Culex , Bidens
15.
J Appl Microbiol ; 131(2): 885-897, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33331046

RESUMO

AIMS: Sclerotinia sclerotiorum, the causal agent of white mold, can infect several host species, including economically important crops. In this study, we propose and validate a new in vitro system able to mimic the conditions of interaction with the host and promote the induction of S. sclerotiorum effectors. METHODS AND RESULTS: For culture media production, we selected three plant species, common bean (Phaseolus vulgaris L, cv. Requinte.), maize (Zea mays, cv. BRS1030) and beggarticks (Bidens pilosa). To validate this system as an in vitro inducer of effectors, the qRT-PCR technique was used to investigate the expression profile of some S. sclerotiorum effector genes in each growth medium at different times after inoculation. CONCLUSION: The results obtained in this study provide a validation of a new method to study S. sclerotiorum during mimetic interaction with different hosts. Although leaf extract does not fully represent the plant environment, the presence of plant components in the culture medium seems to induce effector genes, mimicking in planta conditions. The use of MEVM is simpler than in planta growth, bypasses problems such as the amount of mycelium produced, as well as contamination of host cells during transcriptomic and proteomic analyses. SIGNIFICANCE AND IMPACT OF THE STUDY: We have devised MEVM media as a model mimicking the interaction of S. sclerotiorum and its hosts and used it to evaluate in vitro expression of effectors normally expressed only in planta.


Assuntos
Ascomicetos/genética , Meios de Cultura/química , Doenças das Plantas , Bidens/microbiologia , Regulação Fúngica da Expressão Gênica , Interações Hospedeiro-Patógeno , Phaseolus/microbiologia , Doenças das Plantas/microbiologia , Extratos Vegetais/farmacologia , Proteômica , Transcriptoma , Zea mays/microbiologia
16.
Braz. oral res. (Online) ; 35: e063, 2021. tab, graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1249369

RESUMO

Abstract: FITOPROT, which contains curcuminoids and Bidens pilosa L. extract, is an innovative mucoadhesive formulation indicated for the topical treatment of chemoradiotherapy-induced oral mucositis (OM) in patients with advanced and visible oral squamous cell carcinoma. The formulation is used as a mouthwash directly on tumor tissue of patients with advanced neoplasms, without triggering cancer cell proliferation or tumor invasiveness. Thus, the aim of this study was to evaluate the biological effects of FITOPROT on an oral squamous cell carcinoma cell line (SCC-4). The viability of SCC-4 cells was assessed after exposure to FITOPROT using MTT reduction assay. The effects of the mucoadhesive formulation on cell cycle progression and cell death parameters were evaluated using flow cytometry. In addition, the inflammatory profile of the tumor cells was evaluated using the cytometric bead array (CBA) assay. FITOPROT promoted a concentration-dependent decrease in cell viability and cell cycle arrest at the G2/M phase (p < 0.05). Mitochondrial membrane potential was also altered after exposure to the formulation (p < 0.05), in parallel with a reduction in VEGF and IL-8 production (p = 0.01 and p = 0.05, respectively). In summary, the results indicate that FITOPROT reduces SCC-4 cell viability, promotes cell cycle arrest, modulates mitochondrial membrane potential, and exhibits antiangiogenic and anti-inflammatory properties, thus indicating its potential for topical use in patients with OM and visible tumors in the mouth.


Assuntos
Humanos , Neoplasias Bucais/tratamento farmacológico , Carcinoma de Células Escamosas/tratamento farmacológico , Bidens , Linhagem Celular , Apoptose , Diarileptanoides , Proliferação de Células
17.
Molecules ; 25(21)2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33182287

RESUMO

Plants are considered green resources for thousands of bioactive compounds. Essential oils (EOs) are an important class of secondary compounds with various biological activities, including allelopathic and antimicrobial activities. Herein, the present study aimed to compare the chemical profiles of the EOs of the widely distributed medicinal plant Calotropis procera collected from Saudi Arabia and Egypt. In addition, this study also aimed to assess their allelopathic and antimicrobial activities. The EOs from Egyptian and Saudi ecospecies were extracted by hydrodistillation and analyzed via GC-MS. The correlation between the analyzed EOs and those published from Egypt, India, and Nigeria was assessed by principal component analysis (PCA) and agglomerative hierarchical clustering (AHC). The allelopathic activity of the extracted EOs was tested against two weeds (Bidens pilosa and Dactyloctenium aegyptium). Moreover, the EOs were tested for antimicrobial activity against seven bacterial and two fungal strains. Ninety compounds were identified from both ecospecies, where 76 compounds were recorded in Saudi ecospecies and 33 in the Egyptian one. Terpenes were recorded as the main components along with hydrocarbons, aromatics, and carotenoids. The sesquiterpenes (54.07%) were the most abundant component of EO of the Saudi sample, while the diterpenes (44.82%) represented the mains of the Egyptian one. Hinesol (13.50%), trans-chrysanthenyl acetate (12.33%), 1,4-trans-1,7-cis-acorenone (7.62%), phytol (8.73%), and myristicin (6.13%) were found as the major constituents of EO of the Saudi sample, while phytol (38.02%), n-docosane (6.86%), linoleic acid (6.36%), n-pentacosane (6.31%), and bicyclogermacrene (4.37%) represented the main compounds of the Egyptian one. It was evident that the EOs of both ecospecies had potent phytotoxic activity against the two tested weeds, while the EO of the Egyptian ecospecies was more effective, particularly on the weed D. aegyptium. Moreover, the EOs showed substantial antibacterial and antifungal activities. The present study revealed that the EOs of Egyptian and Saudi ecospecies were different in quality and quantity, which could be attributed to the variant environmental and climatic conditions. The EOs of both ecospecies showed significant allelopathic and antimicrobial activity; therefore, these EOs could be considered as potential green eco-friendly resources for weed and microbe control, considering that this plant is widely grown in arid habitats.


Assuntos
Alelopatia , Anti-Infecciosos/química , Bidens/efeitos dos fármacos , Calotropis/química , Óleos Voláteis/química , Poaceae/efeitos dos fármacos , Antioxidantes/química , Análise por Conglomerados , Ecossistema , Egito , Cromatografia Gasosa-Espectrometria de Massas , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Plantas Daninhas/efeitos dos fármacos , Análise de Componente Principal , Arábia Saudita , Terpenos/química , Compostos Orgânicos Voláteis/química
18.
PLoS One ; 15(10): e0238478, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33112879

RESUMO

Environments in both biotic and abiotic ecosystems have been affected by the colonization of non-native flora. In this study, we examined the effect of Bidens alba invasion on different land-use types along a coastline in southern China. Bacterial communities in each site were determined using 16S rDNA sequencing, and soil physicochemical properties were analyzed using standard methods. Although our results indicated that B. alba invasion did not have a significant effect on the alpha diversity of bacteria, it caused significant differences in soil bacterial community composition between invaded and uninvaded soil across different land-use types. Beta diversity and several physicochemical properties in forest, orchard and waterfront environments were recorded to be more susceptible to B. alba invasion. A high proportion of the variation of bacterial communities can be explained by a combination of environmental variables, indicating that environmental selection rather than plant invasion is a more effective process in coastal microbial assemblages. By comparing topological roles of shared OTUs among invaded and uninvaded soil, keystone taxa in invaded soil were identified. Acidobacteria was the major phyla involved in the invasive process which could be driven by environmental selection. How key phyla react in our experiment should be verified by further studies.


Assuntos
Bidens/fisiologia , Espécies Introduzidas , Microbiologia do Solo , Acidobacteria/genética , Acidobacteria/isolamento & purificação , Biodiversidade , China , Ecossistema , Microbiota/genética , Recursos Naturais , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Solo/química
19.
Plant Dis ; 104(11): 2768-2773, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32915706

RESUMO

Bidens pilosa is an invasive weed that threatens the growth of crops and biodiversity in China. In 2017, suspected bacterial wilt of B. pilosa was discovered in Qinzhou and Beihai, Guangxi, China. A variety of weeds are considered as reservoirs harboring bacterial wilt pathogens, but most do not show obvious symptoms in the field. Identifying the classification status of the B. pilosa bacterial wilt pathogen and exploring its geographical origin might be helpful for clarifying the role of weeds in the circulation of the disease. Phylotyping, sequevar analysis, and cross inoculation of pathogens isolated from B. pilosa and nearby peanut (Arachis hypogaea), balsam gourd (Momordica charantia), and eucalyptus (Eucalyptus robusta) plants were carried out. Three isolates of B. pilosa (Bp01, Bp02, and Bp03) were identified as Ralstonia pseudosolanacearum, race 1, biovar 3, and phylotype I, and belonged to sequevars 17 and 44, and an unknown sequevar. The sequevars isolated from B. pilosa were not completely consistent with those of the nearby hosts, and the virulence of these isolates differed when cross inoculated. The Bp03 sequevar was different from peanut isolate sequevars in the same field and was not identical to any previously designated sequevars. The isolates from B. pilosa and other nearby hosts displayed low or no virulence toward their cross hosts (with wilt incidences less than 33.33%). An exception to this was the isolates from B. pilosa, which displayed high virulence toward eucalyptus (with a wilt incidence of 70.00 to 100.00%). This is the first report of different sequevars of R. pseudosolanacearum causing typical bacterial wilt symptoms in B. pilosa in the field.


Assuntos
Bidens , Ralstonia solanacearum , China , Filogenia , Doenças das Plantas , Ralstonia
20.
Zhongguo Zhong Yao Za Zhi ; 45(16): 3915-3921, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893589

RESUMO

This study aimed to investigate the effect and possible mechanism of Bidens pilosa decoction on non-alcoholic fatty liver disease(NAFLD) induced by high fat and high glucose in mice. Bald/c mice were randomly divided into normal group, model group, metformin(200 mg·kg~(-1)) treatment group, Bidens pilosa decoction(10 g·kg~(-1)) treatment group, metformin and B. pilosa decoction(100 mg·kg~(-1)+5 g·kg~(-1)) treatment group. Except for the normal group, mice in the other four groups were fed with high-fat and high-glucose diet for 8 weeks to establish the non-alcoholic fatty liver model. After 4 weeks of treatment, blood was collected from the eyeballs, the mice were sacrificed, and relevant indicators were detected. The results showed that compared with the model group, blood lipid and blood glucose levels of each treatment group were significantly lower(P<0.05); HE staining results showed that liver pathological damage in each treatment group was significantly improved; oil red O staining results showed fat distribution in each treatment group significantly reduced(P<0.01); immunohistochemical staining showed that glucose regulated the protein expression of protein 78(GRP78) in liver tissues of each treatment group was also significantly reduced(P<0.01); Western blot results showed that endoplasmic reticulum stress signal pathway-related factors GRP78, phosphorylated-protein kinase R-like ER kinase(p-PERK), eukaryotic translation-initiation factor 2α(eIF2α), activating transcription factor 4(ATF4), C/EBP homologous protein(Chop), inositol requiring 1α(IRE1α), and cleaved-cysteinyl aspartate specific proteinase 12(cleaved-caspase-12) were significantly reduced(P<0.01). The results of the combined drug treatment group were better than those of the single drug treatment group. These results showed that B. pilosa decoction had the effect in improving non-alcoholic fatty liver, and its mechanism may be related to the down-regulation of the expression of endoplasmic reticulum stress(ERS)-related factors, and the reduction of the apoptosis of hepatocytes caused by ERS and the down-regulation of blood lipid and blood glucose levels.


Assuntos
Bidens , Hepatopatia Gordurosa não Alcoólica , Animais , Apoptose , Estresse do Retículo Endoplasmático , Endorribonucleases , Glucose , Camundongos , Proteínas Serina-Treonina Quinases
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