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2.
Mater Sci Eng C Mater Biol Appl ; 104: 109984, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31500006

RESUMO

The present investigation highlights the strong antioxidant, anticancer and larvicidal potential of green synthesized silver nanoparticles (AgNPs) using aqueous leaf extract of Piper longum L. for their diverse therapeutic applications. The optimum conditions for the synthesis of AgNPs were recorded as 1 mM AgNO3, 60 ±â€¯2 °C at pH 6 for 120 min. Synthesized AgNPs proved to be highly stable and monodispersed as characterized through various techniques. UV-Vis spectrum of biosynthesized AgNPs showed a maximum absorption peak at 420 nm. Field emission-Scanning electron microscopy (FE-SEM) and High resolution-Transmission electron microscopy (HR-TEM) micrographs showed the spherical shape of AgNPs with mean diameter size of 28.8 nm. Existence of crystallographic AgNPs was proved by X-ray diffraction (XRD) pattern analysis. Presence of phenolics, terpenoids and flavonoids compounds which act as bioreducing agents were confirmed by Fourier-transform infrared spectroscopy (FTIR) analysis. Furthermore, the AgNPs and leaf extracts prepared individually in different solvents such as methanol, ethyl acetate, chloroform, hexane and aqueous were assessed for their bio-efficacies. AgNPs showed the enhanced antioxidant (IC50 67.56 µg) and radical-scavenging activities (IC50 196.8 µg) as compared to the crude leaf extracts. Anticancer activity revealed the strong and dose-dependent cytotoxic effect of AgNPs against the HeLa cells showing maximum IC50 value being 5.27 µg/mL after 24 h and was also found to be non-toxic to normal cells (HEK). The AgNPs induced the fragmentation of DNA in the cells, indicating the occurrence of apoptosis and necrosis. Subsequently, an efficient larvae mortality was also recorded against Anopheles stephensi having LC50 and LC90 values being 8.969 and 16.102 ppm, followed by Aedes aegypti (LC50;14.791 and LC90;28.526 ppm) and Culex quinquefasciatus (LC50;18.662 and LC90;40.903 ppm) after 72 h of exposure. Besides, they showed no toxicity against Mesocyclops thermocyclopoides (non-target organism). This is the first report showing strong anti-tumorous and larvicidal activity of AgNPs synthesized using P. longum leaf extract against cervical cancer cell line and mosquito vectors causing dengue, malaria and filariasis. Based on our findings, we suggest that AgNPs derived using P. longum leaf extract possessed excellent anti-cancerous and mosquito larvicidal potential and therefore, can be bioprospected further for the management of these hazardous health diseases. This study has given a new insight for the novel drug designing after conducting experiments on the in vivo models.


Assuntos
Nanopartículas Metálicas/química , Piper/química , Extratos Vegetais/química , Prata/química , Aedes/efeitos dos fármacos , Animais , Anopheles/efeitos dos fármacos , Bioengenharia/métodos , Linhagem Celular , Linhagem Celular Tumoral , Culex/efeitos dos fármacos , Células HEK293 , Células HeLa , Humanos , Inseticidas/química , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Dose Letal Mediana , Extratos Vegetais/farmacologia
3.
Mater Sci Eng C Mater Biol Appl ; 104: 109892, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31499962

RESUMO

The bone-derived-osteoblast seeded biomaterials scaffold in tissue engineering, have displayed prominence in the treatment of the osseous medical condition. In vitro osteogenesis of rabbit osteoblast cell (rOb) from bone tissue (rT) and MSC-derived rOb from bone marrow (rM) on Gelatin-Hydroxyapatite (HG) based biomaterials was investigated. In this work, lyophilized biomaterial was prepared by the addition of amorphous chitosan ('C') to 'H' dispersed in 'G' matrix, to find its role in biomaterials biocompatibility. Isolated rOb seeded biomaterials were studied using CLSM and flow cytometry for proliferation potential. The biomaterial's core and surface morphology was studied from SEM-EDX and AFM respectively. Upon co-culture with HCG, rT over rM showed rabbit bone extracellular matrix (ECM) mimicking properties both in in vitro studies and biomaterials micro architecture. The in vitro metabolic behaviour was studied by Alamar Blue (AB) assay, DNA content using Hoechst 33258, potency via the activity of Alkaline Phosphatase (ALP), Calcium's relative content by Alizarin Red S (ARS) assay. A novel combination of biomaterials-cell interaction was observed when rT was co-cultured with HCG and proved effective in osteogenesis with regard to Bone Bioengineering.


Assuntos
Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Gelatina/química , Gelatina/farmacologia , Osteoblastos/efeitos dos fármacos , Fosfatase Alcalina/química , Animais , Bioengenharia/métodos , Osso e Ossos/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Quitosana/química , Técnicas de Cocultura/métodos , Durapatita/química , Células-Tronco Mesenquimais/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Coelhos , Engenharia Tecidual/métodos , Tecidos Suporte/química
4.
Nat Commun ; 10(1): 3571, 2019 08 08.
Artigo em Inglês | MEDLINE | ID: mdl-31395864

RESUMO

Oral biofilm accumulation in pets is a growing concern. It is desirable to address this problem via non-invasive teeth cleaning techniques, such as through friction between teeth and food during chewing. Therefore, pet food design tools are needed towards optimising cleaning efficacy. Developing such tools is challenging, as several parameters affecting teeth cleaning should be considered: the food's complex mechanical response, the contacting surfaces topology as well as the wide range of masticatory and anatomical characteristics amongst breeds. We show that Finite Element (FE) models can efficiently account for all these parameters, through the simulation of food deformation and fracture during the first bite. This reduces the need for time consuming and costly in-vivo or in-vitro trials. Our in-silico model is validated through in-vitro tests, demonstrating that the initial oral processing stage can be engineered through computers with high fidelity.


Assuntos
Ração Animal , Bioengenharia/métodos , Mastigação/fisiologia , Higiene Bucal/veterinária , Animais de Estimação/fisiologia , Animais , Biofilmes/crescimento & desenvolvimento , Gatos/microbiologia , Gatos/fisiologia , Simulação por Computador , Cães/microbiologia , Cães/fisiologia , Análise de Elementos Finitos , Mandíbula/anatomia & histologia , Mandíbula/fisiologia , Maxila/anatomia & histologia , Maxila/fisiologia , Modelos Biológicos , Animais de Estimação/microbiologia , Dente/anatomia & histologia , Dente/microbiologia , Dente/fisiologia
5.
Nat Commun ; 10(1): 3873, 2019 08 27.
Artigo em Inglês | MEDLINE | ID: mdl-31455784

RESUMO

Development of the next generation of bio- and nano-electronics is inseparably connected to the innovative concept of emulation and reproduction of biological sensorimotor systems and artificial neurobotics. Here, we report for the first time principally new artificial bioinspired optoelectronic sensorimotor system for the controlable immitation of opto-genetically engineered neurons in the biological motor system. The device is based on inorganic optical synapse (In-doped TiO2 nanofilm) assembled into a liquid metal (galinstan) actuator. The optoelectronic synapse generates polarised excitatory and inhibitory postsynaptic potentials to trigger the liquid metal droplet to vibrate and then mimic the expansion and contraction of biological fibre muscle. The low-energy consumption and precise modulation of electrical and mechanical outputs are the distinguished characteristics of fabricated sensorimotor system. This work is the underlying significant step towards the development of next generation of low-energy the internet of things for bioinspired neurorobotic and bioelectronic system.


Assuntos
Bioengenharia/métodos , Optogenética/métodos , Robótica/instrumentação , Ligas/química , Sinapses Elétricas/fisiologia , Técnicas Eletroquímicas/métodos , Potenciais Pós-Sinápticos Inibidores/fisiologia , Metais Pesados/química , Músculos/inervação , Músculos/fisiologia , Neurônios/fisiologia
6.
Sci Total Environ ; 693: 133677, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31377348

RESUMO

Soil loss and erosion is a major environmental problem in the Mediterranean. Soil and water bioengineering uses plants and/or parts of plants along with inert material to create solutions to fulfill soil conservation objectives combined with an ecological rehabilitation approach. The ECOMED project developed novel approaches and tools to specialize the soil and water bioengineering sector within the Mediterranean. The first activity was the Sector Needs Analysis were the responses to an online questionnaire of 110 stakeholders from the region were analyzed. The main conclusion was the need to specialize the soil and water bioengineering sector in the Mediterranean. In addition, 21 soil and water bioengineering case studies in the Mediterranean were reviewed. Many works of this type are implemented in the region, but have flaws because of the lack of training material, design routines, protocols, specific to the region. The second activity developed New Design Routines and Protocols. Specifically, three protocols, one template and one plant database were developed for the region. Finally, in the Training Material activity, six educational modules along with a handbook (that contained modules, protocols, template and case studies) were developed. Overall the ECOMED project generated new and novel material and tools that were lacking in the region to enhance the specialization process of the soil and water bioengineering sector. These should increase the adoption of soil and water bioengineering techniques with better trained and new professionals as well as improve the work performance of these interventions.


Assuntos
Bioengenharia/métodos , Agricultura , Conservação dos Recursos Naturais/métodos , Ecologia , Plantas , Solo , Água
7.
Nature ; 572(7768): 224-229, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31391558

RESUMO

Living systems are capable of locomotion, reconfiguration and replication. To perform these tasks, cells spatiotemporally coordinate the interactions of force-generating, 'active' molecules that create and manipulate non-equilibrium structures and force fields of up to millimetre length scales1-3. Experimental active-matter systems of biological or synthetic molecules are capable of spontaneously organizing into structures4,5 and generating global flows6-9. However, these experimental systems lack the spatiotemporal control found in cells, limiting their utility for studying non-equilibrium phenomena and bioinspired engineering. Here we uncover non-equilibrium phenomena and principles of boundary-mediated control by optically modulating structures and fluid flow in an engineered system of active biomolecules. Our system consists of purified microtubules and light-activatable motor proteins that crosslink and organize the microtubules into distinct structures upon illumination. We develop basic operations-defined as sets of light patterns-to create, move and merge the microtubule structures. By combining these operations, we create microtubule networks that span several hundred micrometres in length and contract at speeds up to an order of magnitude higher than the speed of an individual motor protein. We manipulate these contractile networks to generate and sculpt persistent fluid flows. The principles of boundary-mediated control that we uncover may be used to study emergent cellular structures and forces and to develop programmable active-matter devices.


Assuntos
Bioengenharia/métodos , Cinesina/metabolismo , Cinesina/efeitos da radiação , Luz , Microtúbulos/química , Microtúbulos/efeitos da radiação , Cinesina/química , Microtúbulos/metabolismo
8.
J Appl Microbiol ; 127(4): 1125-1134, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31336395

RESUMO

AIM: Escherichia coli is the conventional choice as the host strain for whole-cell bioreporter construction due to its well-understood genetics and well-established cloning protocols. However, for real-world environmental biosensing applications, it is often beneficial to use a bacterial strain derived directly from the environment under study to better ensure chemical target specificity and optimal response time. The aim of this study was to develop a whole-cell bioreporter for detection of bioavailable mercury by replacing E. coli with a wild-type bacterial host derived from a soil environment. MATERIALS AND RESULTS: In this study, an Enterobacter cloacae strain isolated from soil derived from a municipal and electronic waste dumping site was engineered to serve as a bioluminescent bioreporter for mercury toxicity by linking its merR-like gene and promoter sequence to a reorganized luxABCDE gene cassette from Photorhabdus luminescens. This bioreporter, designated as E. cloacae DWH4lux , detected mercury (HgCl2 ) at a minimum concentration of 0·2 µg l-1 with a linear response profile being maintained between a range of 0·4-1600 µg l-1 (R2  = 0·9604) with a peak bioluminescent response occurring within 1 h after exposure. No significant synergistic or antagonistic influences were observed on the bioluminescent response by other contaminating metal elements. Enterobacter cloacae DWH4lux was also demonstrated to detect mercury effectively in artificially contaminated water sample with linear correlation (R2  = 0·9623). CONCLUSIONS: The results indicated that E. cloacae DWH4lux could detect mercury in quantities below the US Environmental Protection Agency's permitted limit values (2 µg l-1 ). Hence, it is concluded that E. cloacae DWH4lux has the potential to serve as an effective whole-cell bioreporter for the environmental monitoring of mercury contamination. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides new insight into the recruitment of mercury-tolerant bacterial hosts derived from environmental samples over the conventional lab-based E. coli host for the construction of mercury bioreporters. With improved response time and selectivity, the environmentally sourced bacteria can serve as an alternative host choice to improve biosensing technology in the near future.


Assuntos
Bioengenharia/métodos , Enterobacter cloacae , Monitoramento Ambiental/métodos , Mercúrio/análise , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Enterobacter cloacae/genética , Enterobacter cloacae/metabolismo , Medições Luminescentes , Photorhabdus/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Microbiologia do Solo
9.
Appl Microbiol Biotechnol ; 103(15): 6107-6117, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31187211

RESUMO

Noncoding RNAs (ncRNAs), including microRNAs (miRNAs), small interfering RNAs (siRNAs), and long noncoding RNAs (lncRNAs), regulate target gene expression and can be used as tools for understanding biological processes and identifying new therapeutic targets. Currently, ncRNA molecules for research and therapeutic use are limited to ncRNA mimics made by chemical synthesis. We have recently established a high-yield and cost-effective method of producing bioengineered or biologic ncRNA agents (BERAs) through bacterial fermentation, which is based on a stable tRNA/pre-miR-34a carrier (~ 180 nt) that accommodates target small RNAs. Nevertheless, it remains a challenge to heterogeneously express longer ncRNAs (e.g., > 260 nt), and it is unknown if single BERA may carry multiple small RNAs. To address this issue, we hypothesized that an additional human pre-miR-34a could be attached to the tRNA/pre-miR-34a scaffold to offer a new tRNA/pre-miR-34a/pre-miR-34a carrier (~ 296 nt) for the accommodation of multiple small RNAs. We thus designed ten different combinatorial BERAs (CO-BERAs) that include different combinations of miRNAs, siRNAs, and antagomirs. Our data showed that all target CO-BERAs were successfully expressed in Escherichia coli at high levels, greater than 40% in total bacterial RNAs. Furthermore, recombinant CO-BERAs were purified to a high degree of homogeneity by fast protein liquid chromatography methods. In addition, CO-BERAs exhibited strong anti-proliferative activities against a variety of human non-small cell lung cancer cell lines. These results support the production of long ncRNA molecules carrying different warhead small RNAs for multi-targeting which may open avenues for developing new biologic RNAs as experimental, diagnostic, and therapeutic tools.


Assuntos
Antagomirs/biossíntese , Antagomirs/genética , Bioengenharia/métodos , RNA Longo não Codificante/biossíntese , RNA Longo não Codificante/genética , RNA Interferente Pequeno/biossíntese , RNA Interferente Pequeno/genética , Cromatografia Líquida , Escherichia coli/genética , Escherichia coli/metabolismo , Expressão Gênica , Humanos , RNA Longo não Codificante/isolamento & purificação
11.
Science ; 364(6439)2019 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-31048459

RESUMO

An important goal in synthetic biology is to engineer biochemical pathways to address unsolved biomedical problems. One long-standing problem in molecular medicine is the specific identification and ablation of cancer cells. Here, we describe a method, named Rewiring of Aberrant Signaling to Effector Release (RASER), in which oncogenic ErbB receptor activity, instead of being targeted for inhibition as in existing treatments, is co-opted to trigger therapeutic programs. RASER integrates ErbB activity to specifically link oncogenic states to the execution of desired outputs. A complete mathematical model of RASER and modularity in design enable rational optimization and output programming. Using RASER, we induced apoptosis and CRISPR-Cas9-mediated transcription of endogenous genes specifically in ErbB-hyperactive cancer cells. Delivery of apoptotic RASER by adeno-associated virus selectively ablated ErbB-hyperactive cancer cells while sparing ErbB-normal cells. RASER thus provides a new strategy for oncogene-specific cancer detection and treatment.


Assuntos
Apoptose/genética , Bioengenharia/métodos , Neoplasias/genética , Neoplasias/terapia , Receptor ErbB-2/antagonistas & inibidores , Receptor ErbB-2/genética , Adenoviridae , Proteína 9 Associada à CRISPR , Sistemas CRISPR-Cas , Linhagem Celular Tumoral , Endopeptidases/genética , Humanos , Modelos Teóricos , Neoplasias/patologia , Estabilidade Proteica , Proteólise , Receptor ErbB-2/metabolismo , Transdução de Sinais , Biologia Sintética , Transcrição Genética , Proteínas não Estruturais Virais/genética
13.
PLoS One ; 14(4): e0213847, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30990818

RESUMO

Bioengineering, which studies the principles and design of biological systems, is a field that has inspired the development of several technologies that are currently in use. In this work, we use concepts from the fish lateral line sensing mechanism and apply them to seismic imaging processing. The lateral line is a sensory system composed of an integrated array of mechanical sensors spanning along the fish body. We compare the array of sensors along body fish with the seismic acquisition, which employs an array of equally spaced identical mechanical sensors to image the Earth's subsurface. In both situations, the mechanical sensors capture and process mechanical vibrations from the environment to produce useful information. We explore the strategy of using the low-pass and high-pass sensors schema of fish lateral line to improve the seismic technique. We use the full-wave inversion method to compare the conventional acquisition procedure of identical sensors with alternative sets of different sensors, which mimics the fish lateral line. Our results show that the alternate sensors arrangement surpasses the performance of the conventional acquisition method, using just half of the input information. The results point at an image processing technique that is computationally more efficient and economical than the usual seismic processing method.


Assuntos
Bioengenharia/métodos , Desastres/prevenção & controle , Terremotos , Monitoramento Ambiental/instrumentação , Mecanorreceptores/fisiologia , Animais , Monitoramento Ambiental/métodos , Desenho de Equipamento , Peixes/fisiologia , Geografia , Processamento de Imagem Assistida por Computador , Sistema da Linha Lateral/citologia , Sistema da Linha Lateral/fisiologia , Sistemas Microeletromecânicos
15.
Plast Reconstr Surg ; 143(6): 1648-1655, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30907806

RESUMO

BACKGROUND: Fat grafting is a common procedure in plastic surgery. A major limitation is unpredictable graft retention, in part caused by inadequate oxygen delivery during the early posttransfer period. METHODS: The authors present a bioengineered approach to the design of a fat graft based on mathematical theory, which can estimate the limitations of oxygen delivery. To simplify the problem, four variables were defined: (1) recipient-site oxygen partial pressure; (2) adipose tissue oxygen permeability; (3) adipose tissue oxygen consumption rate; and (4) fat graft size. Recipient-site oxygen partial pressure and adipose tissue oxygen permeability were estimated from literature, whereas adipose tissue oxygen consumption rate was measured using stirred microchamber technology. Calculations were performed in both spherical and planar geometry to calculate the maximum allowable fat graft size from an oxygen delivery standpoint. RESULTS: As expected, planar geometry is less favorable for oxygenation but represents a realistic configuration for a fat graft. Maximum allowable fat graft thickness is only approximately 1 to 2 mm at external oxygen partial pressures of 10 to 40 mm Hg; any thicker and an anoxic or necrotic core likely develops. Given a reasonably large surface area and assuming several planes of injection, the maximum allowable fat graft volume is tens of milliliters. CONCLUSIONS: A systematic bioengineered approach may help better design a fat graft. Applying principles of mass transfer theory can predict whether a fat graft has a favorable chance of surviving from an oxygen delivery standpoint and can direct the development of strategies for improved fat graft oxygenation.


Assuntos
Tecido Adiposo/transplante , Bioengenharia/métodos , Consumo de Oxigênio/fisiologia , Cirurgia Plástica/métodos , Sobrevivência de Enxerto , Humanos , Modelos Teóricos , Valor Preditivo dos Testes , Transplante de Tecidos/métodos , Coleta de Tecidos e Órgãos/métodos
16.
Mar Drugs ; 17(3)2019 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-30889823

RESUMO

In recent years, the demand for naturally derived products has hiked with enormous pressure to propose or develop state-of-the-art strategies to meet sustainable circular economy challenges. Microalgae possess the flexibility to produce a variety of high-value products of industrial interests. From pigments such as phycobilins or lutein to phycotoxins and several polyunsaturated fatty acids (PUFAs), microalgae have the potential to become the primary producers for the pharmaceutical, food, and agronomical industries. Also, microalgae require minimal resources to grow due to their autotrophic nature or by consuming waste matter, while allowing for the extraction of several valuable side products such as hydrogen gas and biodiesel in a single process, following a biorefinery agenda. From a Mexican microalgae biodiversity perspective, more than 70 different local species have been characterized and isolated, whereas, only a minimal amount has been explored to produce commercially valuable products, thus ignoring their potential as a locally available resource. In this paper, we discuss the microalgae diversity present in Mexico with their current applications and potential, while expanding on their future applications in bioengineering along with other industrial sectors. In conclusion, the use of available microalgae to produce biochemically revenuable products currently represents an untapped potential that could lead to the solution of several problems through green technologies. As such, if the social, industrial and research communities collaborate to strive towards a greener economy by preserving the existing biodiversity and optimizing the use of the currently available resources, the enrichment of our society and the solution to several environmental problems could be attained.


Assuntos
Biodiversidade , Bioengenharia/métodos , Produtos Biológicos/química , Microalgas/química , Crescimento Sustentável , Biocombustíveis , Biomassa , México
17.
Pest Manag Sci ; 75(9): 2368-2377, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30884099

RESUMO

Plants are sessile organisms that have evolved various mechanisms to adapt to complex and changing environments. One important feature of plant adaption is the production of specialised metabolites. Terpenes are the largest class of specialised metabolites, with over 80 000 structures reported so far, and they have important ecological functions in plant adaptation. Here, we review the current knowledge on plant terpenes that mediate below-ground interactions between plants and other organisms, including microbes, herbivores and other plants. The discovery, functions and biosynthesis of these terpenes are discussed, and prospects for bioengineering terpenoids for plant protection are considered. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.


Assuntos
Bioengenharia/métodos , Proteção de Cultivos/métodos , Plantas/metabolismo , Terpenos/metabolismo
19.
Int J Cardiol ; 283: 67-72, 2019 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-30826192

RESUMO

BACKGROUND: The COMBO stent (OrbusNeich Medical, Ft. Lauderdale, Florida) is a new-generation bio-engineered drug eluting stent, combining an abluminal coating of a bioabsorbable polymer matrix for sustained release of sirolimus and luminal anti-CD34 coating for endothelial progenitor cell capture and rapid endothelialization. METHODS: The Multinational Abluminal Sirolimus Coated BiO-Engineered StenT (MASCOT) registry was a prospective post-marketing study conducted from June 2014-May 2017 across 60 centers globally. Patients were eligible if COMBO stent implantation was attempted, and they received dual antiplatelet therapy (DAPT) per local guidelines. Follow-up was conducted by trained research staff at 1, 6 and 12 months by phone or clinic visit to capture clinical events and DAPT cessation events. The primary endpoint was 1-year target lesion failure (TLF), composite of cardiac death, non-fatal myocardial infarction not clearly attributable to a non-target vessel, or ischemia-driven target lesion revascularization. RESULTS: A total of 2614 patients were enrolled over the study period with 96.7% completion of 1-year follow-up. The mean age of enrolled patients was 62.9 ±â€¯11.2 years and 23.0% were female. Diabetes mellitus was present at baseline in 33.5%. A total of 56.1% patients underwent PCI for acute coronary syndrome (ACS). The 1-year primary endpoint of TLF occurred in 3.4% patients (n = 88). Definite stent thrombosis occurred in 0.5% patients (n = 12). CONCLUSION: The MASCOT post marketing registry provides comprehensive safety and efficacy outcomes following contemporary PCI using the novel COMBO stent in an all-comer population. This platform is associated with low rates of 1-year TLF and ST. CLINICALTRIALS. GOV IDENTIFIER: NCT02183454.


Assuntos
Doença da Artéria Coronariana/cirurgia , Stents Farmacológicos , Intervenção Coronária Percutânea/métodos , Vigilância de Produtos Comercializados/estatística & dados numéricos , Sistema de Registros , Sirolimo/farmacologia , Bioengenharia/métodos , Feminino , Seguimentos , Humanos , Imunossupressores/farmacologia , Masculino , Pessoa de Meia-Idade , Desenho de Prótese , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento
20.
Curr Pharm Biotechnol ; 20(1): 32-46, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30727886

RESUMO

Osteoarthritis (OA) is a common degenerative disease which involves articular cartilage, and leads to total joint disability in the advanced stages. Due to its avascular and aneural nature, damaged cartilage cannot regenerate itself. Stem cell therapy and tissue engineering represent a promising route in OA therapy, in which cooperation of mesenchymal stem cells (MSCs) and three-dimensional (3D) scaffolds contribute to cartilage regeneration. However, this approach still presents some limits such as poor mechanical properties of the engineered cartilage. The natural dynamic environment of the tissue repair process involves a collaboration of several signals expressed in the biological system in response to injury. For this reason, tissue engineering involving exogenous "influencers" such as mechanostimulation and functional biomolecule delivery systems (BDS), represent a promising innovative approach to improve the regeneration process. BDS provide a controlled release of biomolecules able to interact between them and with the injured tissue. Nano-dimensional BDS is the future hope for the design of personalized scaffolds, able to overcome the delivery problems. MSC-derived extracellular vesicles (EVs) represent an attractive alternative to BDS, due to their innate targeting abilities, immunomodulatory potential and biocompatibility. Future advances in cartilage regeneration should focus on multidisciplinary strategies such as modular assembly strategies, EVs, nanotechnology, 3D biomaterials, BDS, mechanobiology aimed at constructing the functional scaffolds for actively targeted biomolecule delivery. The aim of this review is to run through the different approaches adopted for cartilage regeneration, with a special focus on biomaterials, BDS and EVs explored in terms of their delivery potential, healing capabilities and mechanical features.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Bioengenharia/métodos , Cartilagem Articular/fisiologia , Sistemas de Liberação de Medicamentos/métodos , Osteoartrite/terapia , Regeneração/fisiologia , Animais , Bioengenharia/tendências , Cartilagem Articular/efeitos dos fármacos , Sistemas de Liberação de Medicamentos/tendências , Humanos , Transplante de Células-Tronco Mesenquimais/métodos , Transplante de Células-Tronco Mesenquimais/tendências , Células-Tronco Mesenquimais/fisiologia , Regeneração/efeitos dos fármacos , Engenharia Tecidual/métodos , Engenharia Tecidual/tendências , Tecidos Suporte/tendências
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