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1.
In. Caribbean Public Health Agency. Caribbean Public Health Agency: 60th Annual Scientific Meeting. Kingston, The University of the West Indies. Faculty of Medical Sciences, 2015. p.[1-75]. (West Indian Medical Journal Supplement).
Monografia em Inglês | MedCarib | ID: med-18064

RESUMO

OBJECTIVE: This study formed part of a soil transmitted helminth (STH) elimination program in Grenada. This study assessed the short and long term knowledge of primary school children on the transmission and prevention of STH using Turning Point Technologies. DESIGN AND METHODS: A longitudinal study was carried out on schoolchildren attending 39 out of all 54 (72.2%) primary schools from Grenada, Carriacou and Petite Martinique between March and November 2014. The schools were randomly selected for inclusion in the study. Turning Point Technology was used to assess the knowledge and administer the subsequent intervention to all students. The Pre, immediate-Post and long-term Post intervention (seven months later) data was analyzed using Chi Square. RESULTS: A total of 962 students participated in March 2014 and 805 students participated in October 2014. There was no significant (p< 0.05) inter school difference in the answering of questions. This displayed a ubiquitous knowledge transfer across the primary schools. There was a significant improvement in knowledge post turning point intervention, which was maintained at 7-months post-intervention. CONCLUSION: The advantages of using turning point technologies to impart this knowledge where class responses appear instantly, anonymously and can be used for immediate discussion were numerous. These include inter alia, active engaged participation.


Assuntos
Conhecimento , Criança , Helmintos , Biologia do Solo/prevenção & controle , Granada
2.
In. Caribbean Public Health Agency. Caribbean Public Health Agency: 60th Annual Scientific Meeting. Kingston, The University of the West Indies. Faculty of Medical Sciences, 2015. p.[1-75]. (West Indian Medical Journal Supplement).
Monografia em Inglês | MedCarib | ID: med-18065

RESUMO

OBJECTIVE: To identify the attitude, behaviour and practice of parents, guardians and adolescents associated with Soil Transmitted Helminth’s (STH’s). DESIGN AND METHODS: A questionnaire was completed by 811 parents /guardians. The questionnaire posed questions on the socio economic, attitudes, behavior and practices of primary school students with regard to factors that could contribute to the transmission of STH’s in Grenada. RESULTS: 534 (66%) parents/guardian reported that their children regularly washed their hands before eating, 690 (85%) had an inside water supply, 465 (57%) had indoor toilets. Also, 579 (71%) said that their children never played around outdoor latrines and 326 (40%) said their children always wore shoes outdoors. 732 (90%) of the parents/guardians reported that vegetables and fruits were always washed before cooking and 662 (82%) said that their children had previously received de-worming medication. CONCLUSION: Practical recommendations such as in-house sanitation facilities and hand washing programs can be made. This will enable limited resources to be used more efficiently to combat the problem of transmission of STH’s.


Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Criança , Biologia do Solo , Helmintos , Granada
3.
Kasmera ; 36(1): 17-27, ene.-jun. 2008. ilus
Artigo em Espanhol | LILACS | ID: lil-517669

RESUMO

Se evaluó la coloración diferencial de fluorescencia modificada en Pseudomonas spp. aisladas de suelos de cultivos agrícolas del estado Sucre, a fin de observar eventos microscópicos relacionados con el ciclo celular. Cada especie de Pseudomonas identificada bioquímicamente se sembró en caldos incubados a temperatura ambiente, aerobiosis, durante 15, 20, 30 y 45 minutos, y 1, 24, 48 y 72 horas; luego, se elaboraron y colorearon los extendidos. En las 24 cepas de Pseudomonas identificadas, P. mendocina (41,67 por ciento), P. aeruginosa (37,50 por ciento) y P. putida (20,83 por ciento), se observaron variaciones de tinción en los diferentes tiempos de incubación como verde, amarilla y anaranjada, fluorescentes y de baja fluorescencia. La coloración emplea naranja de acridina que se intercala al ADN, provocando fluorescencia verde, e interactúa con el ARN provocando fluorescencia anaranjada; el decolorante remueve el naranja de acridina no unido al material genético y la fluoresceína de sodio produce color amarillo en bacterias que retienen suficiente cantidad de naranja de acridina. Las variaciones de tinción citoplasmática en Pseudomonas spp., están asociadas a la cantidad de ARN y ADN presente en la célula de acuerdo a la fase de su ciclo celular.


The modified fluorescence staining differential was evaluated using Pseudomonas spp. isolated from cultivated agricultural soils in the State of Sucre, in order to observe microscopic events related to the cellular cycle. Each species of biochemically identified Pseudomonas was inoculated into a broth and incubated at room temperature, aerobiosis, for 15, 20, 30 and 45 minutes and 1, 24, 48 and 72 hours; then, slides were made and stained. For the 24 identified strains of Pseudomonas, P. mendocina (41.67 percent), P. aeruginosa (37.50 percent) and P. putida (20.83 percent), staining variations such as green, yellow and orange, fluorescent and low fluorescence were observed for the different incubation times. The stain uses acridine orange that interacts with DNA by intercalation, causing green fluorescence; it interacts with RNA by electrostatic attraction causing orange fluorescence; the alcohol-acetone decolorant removes the acridine orange not united with the genetic material and sodium fluorescein produces a yellow color in bacteria that retain a sufficient amount of acridine orange. Cytoplasmatic staining variations in Pseudomonas spp., are associated with the amount of RNA and DNA present in the cells according to the phase of their cellular cycle.


Assuntos
Laranja de Acridina/análise , Laranja de Acridina/química , Fluorescência , Pseudomonas/classificação , Pseudomonas/química , Análise do Solo , Microbiologia , Biologia Molecular , Biologia do Solo
4.
Int. microbiol ; 8(1): 13-21, mar. 2005. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-038284

RESUMO

Soil health refers to the biological, chemical, and physical features of soil that are essential to long-term, sustainable agricultural productivity with minimal environmental impact. Thus, soil health provides an overall picture of soil functionality. Although it cannot be measured directly, soil health can be inferred by measuring specific soil properties (e.g. organic matter content) and by observing soil status (e.g. fertility). There is also increased interest in studying soil microorganisms in their particular environments, as microbial diversity is intimately related to soil structure and function. One of the key objectives in determining soil health is to acquire indicators that can be used to evaluate the soil’s current status and hence to develop sustainable agricultural systems. In this regard, significant progress has been made over the last few years in the development of specific biomarkers and macromolecular probes, enabling rapid and reliable measurements of soil microbial communities. In addition, modern molecular biological techniques, such as fluorescence in situ hybridization (FISH), reverse transcriptase polymerase chain reaction (RT-PCR), denaturing gradient gel electrophoresis (DGGE), and terminal restriction fragment length polymorphism (T-RFLP), have facilitated the analysis of microbial biodiversity and activity, whereas the application of modern analytical techniques, such as nuclear magnetic resonance (NMR) and pyrolysis gas chromatography-mass spectrometry (Py-GC-MS), have provided data on soil chemistry. The combination of these two approaches offers promise in determinings oil health status (AU)


El concepto de salud del suelo se relaciona con las características biológicas, químicas y físicas que son esenciales para una productividad agrícola sostenible a largo plazo con un mínimo impacto ambiental. La salud del suelo es el más fiel reflejo de su funcionalidad. Aunque no puede medirse directamente, se puede inferir a partir de la determinación de propiedades específicas del mismo suelo (p.e. el contenido en materia orgánica) y por la observación de su estado (p.e. la fertilidad). El interés por el estudio de los microorganismos del suelo en su propio medio está aumentando, puesto que la diversidad microbiana está estrechamente relacionada con la estructura y función del suelo. Un objetivo clave para determinar la salud de un suelo es la disponibilidad de indicadores factibles de ser utilizados en la evaluación de su estado y, a partir de aquí, implementar sistemas de agricultura sostenible. El desarrollo de biomarcadores específicos y sondas macromoleculares ha evolucionado considerablemente en los últimos años, lo cual ha permitido obtener medidas fiables y rápidas de las comunidades microbianas del suelo. Por otro lado, las modernas técnicas biomoleculares más recientes (p.e. la hibridación in situ mediante fluorescencia [FISH], la reacción en cadena de la polimerasa mediante transcriptasa inversa [RT-PCR], la electroforesis en gel con gradiente desnaturalizante [DGGE]y el análisis del polimorfismo en la longitud de los fragmentos de restricción terminales [T-RFLP]) permiten analizar la biodiversidad y actividad microbianas, mientras que la aplicación de técnicas analíticas modernas (p.e. resonancia magnética nuclear [NMR], pirolisis-cromatografía de gases-espectrometría de masas [Py-GC-MS]) proporciona datos sobre la química del suelo. La combinación de estas dos aproximaciones metodológicas ofrece buenas perspectivas en la determinación del estado de salud del suelo (AU)


Assuntos
Microbiologia do Solo , Análise do Solo , Biologia do Solo , Características do Solo , Condições do Solo , Biomassa , Biodiversidade , Cultivos Agrícolas
6.
An. R. Acad. Farm ; 70(3): 743-776, jul. 2004.
Artigo em Espanhol | IBECS | ID: ibc-36135

RESUMO

Se pone de relieve la importancia de los microorganismos del suelo, que establecen simbiosis con las plantas como biofertilizantes y su aplicación en el control biológico de patógenos. Las interacciones beneficiosas: bacteria-planta y hongo-planta tienen gran interés por su impacto en la Agricultura, Silvicultura y Medioambiente y constituyen una alternativa a la aplicación de fertilizantes químicos que actúan como contaminantes de suelos y aguas con gran perjuicio para la salud. Las bacterias fijadoras de nitrógeno y los hongos micorrizógenos se encuentran entre los simbiontes de plantas más extendidos y ecológicamente más importantes. El potencial de los microorganismos del suelo parece ilimitado. Corresponde a la ciencia realizar el estudio profundo de las interacciones de organismos autóctonos del suelo con las plantas, con el fin de que éstas puedan autoabastescerse y autodefenderse en condiciones ambientales adversas, y además se cumpla con el deber de mantener nuestro planeta en óptimas condiciones de salud ambiental para las generaciones futuras (AU)


Assuntos
Fertilizantes/análise , Controle Biológico de Vetores/métodos , Rhizobiaceae , Biologia do Solo , Nitrogênio/análise , Microbiologia do Solo , Simbiose , Micorrizas , Fabaceae
7.
In. Ahmad, Rafi, ed. Natural hazards and hazard management in the greater Caribbean and Latin America : Proceedings of the second caribbean conference on natural hazards and disasters held in Kingston, Jamaica. Kingston, Jamaica. University of the West Indies. Unit for Disaster Studies, 1997. p.112-23, mapas, tab. (Publication, 3).
Monografia em Inglês | Desastres | ID: des-10737

RESUMO

Landslides are verry common on Jamaican roads, and the consequences of these slides are costly. We have corried out a rapid assessment of landslides hazard alone the Guinea Corn to Corner Shop Road, via Johns Hall in Central jamaican identify road sections where bio-engineering may be used for the effective road maintenance. This road is subject to recurrent landslide activity and flooding following every significant rainfall event in the Mahoe River watershed. The existing vegetation types along the road have been described in term of their fuction in arresting slope movements and the protective cover they offer against infiltration. In areas where vegetation cover is inadequate or instability observed, recommendations are made as to vegetation types and techniques which may be implemented for slope stabilization. Many of the initiatives currently practiced by local population alrealy make a positive contribution to road-side stabilization. This research programme has been initiated in jamaica jointly by Natural Resoreces Institute (UK) and Departments og Geography and Geology, and Life Sciences, University of the West Indies, Mona. (AU)


Assuntos
Deslizamentos de Terra , Biologia do Solo , Engenharia , Estabilização , Jamaica , Geografia , Geologia
8.
Buenos Aires; El Ateneo; 5a. ed; 1984. 499 p. ilus, tab.
Monografia em Espanhol | BINACIS | ID: bin-135725
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