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2.
Ann Biol Clin (Paris) ; 78(3): 269-277, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: covidwho-600996

RESUMO

The SARS-CoV-2 virus is responsible for an epidemic disease called COVID-19, which was initially evidenced in Wuhan, China, and spread very rapidly in China and around the world. In France, the first isolated case seems now to be reported in December 2019, stage 3 of the COVID-19 epidemic was triggered on March 14th, the start of the planned containment exit from May 11th. Healthcare services have faced a large influx of patients who may be beyond their capacity to receive and care, particularly in the Large-East and Ile-de-France regions. Some patients show an evolution of the disease never observed before with other coronaviruses and develop in a few days a very important inflammatory reaction, which can lead to death of patients. A working group of the French Society of Clinical Biology (SFBC) was set up with the objective of providing updated information on the current status of the biological prescriptions (focusing on biochemistry ones) and their evolution during the epidemic, and of analyzing the biological parameters associated with comorbidities and patient evolution in order to link biological results with medical events. The expanded working group covers all sectors of medical biology in France and extends to the French-speaking world: hospital sectors (CHU and CH, Army Training Hospitals) and the private sector opening a field of view on the biological situation in establishments for dependent elderly, social establishments and clinical medical institutions. The purpose of this article is the presentation of this working group and its immediate and future actions.


Assuntos
Betacoronavirus , Bioquímica/organização & administração , Biomarcadores/análise , Serviços de Laboratório Clínico/organização & administração , Infecções por Coronavirus/diagnóstico , Pneumonia Viral/diagnóstico , Sociedades Científicas/organização & administração , Betacoronavirus/isolamento & purificação , Betacoronavirus/patogenicidade , Bioquímica/normas , Biomarcadores/sangue , Serviços de Laboratório Clínico/normas , Redes Comunitárias/organização & administração , Redes Comunitárias/normas , Redes Comunitárias/tendências , Infecções por Coronavirus/sangue , Infecções por Coronavirus/epidemiologia , Surtos de Doenças , França/epidemiologia , História do Século XXI , Humanos , Colaboração Intersetorial , Pandemias , Pneumonia Viral/sangue , Pneumonia Viral/epidemiologia , Prática Profissional/organização & administração , Prática Profissional/normas , Prática Profissional/tendências , Sociedades Científicas/normas , Comunicação por Videoconferência/organização & administração , Comunicação por Videoconferência/normas
3.
Ann Biol Clin (Paris) ; 78(3): 269-277, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: covidwho-600995

RESUMO

The SARS-CoV-2 virus is responsible for an epidemic disease called COVID-19, which was initially evidenced in Wuhan, China, and spread very rapidly in China and around the world. In France, the first isolated case seems now to be reported in December 2019, stage 3 of the COVID-19 epidemic was triggered on March 14th, the start of the planned containment exit from May 11th. Healthcare services have faced a large influx of patients who may be beyond their capacity to receive and care, particularly in the Large-East and Ile-de-France regions. Some patients show an evolution of the disease never observed before with other coronaviruses and develop in a few days a very important inflammatory reaction, which can lead to death of patients. A working group of the French Society of Clinical Biology (SFBC) was set up with the objective of providing updated information on the current status of the biological prescriptions (focusing on biochemistry ones) and their evolution during the epidemic, and of analyzing the biological parameters associated with comorbidities and patient evolution in order to link biological results with medical events. The expanded working group covers all sectors of medical biology in France and extends to the French-speaking world: hospital sectors (CHU and CH, Army Training Hospitals) and the private sector opening a field of view on the biological situation in establishments for dependent elderly, social establishments and clinical medical institutions. The purpose of this article is the presentation of this working group and its immediate and future actions.


Assuntos
Betacoronavirus , Bioquímica/organização & administração , Biomarcadores/análise , Serviços de Laboratório Clínico/organização & administração , Infecções por Coronavirus/diagnóstico , Pneumonia Viral/diagnóstico , Sociedades Científicas/organização & administração , Betacoronavirus/isolamento & purificação , Betacoronavirus/patogenicidade , Bioquímica/normas , Biomarcadores/sangue , Serviços de Laboratório Clínico/normas , Redes Comunitárias/organização & administração , Redes Comunitárias/normas , Redes Comunitárias/tendências , Infecções por Coronavirus/sangue , Infecções por Coronavirus/epidemiologia , Surtos de Doenças , França/epidemiologia , História do Século XXI , Humanos , Colaboração Intersetorial , Pandemias , Pneumonia Viral/sangue , Pneumonia Viral/epidemiologia , Prática Profissional/organização & administração , Prática Profissional/normas , Prática Profissional/tendências , Sociedades Científicas/normas , Comunicação por Videoconferência/organização & administração , Comunicação por Videoconferência/normas
4.
Ann Biol Clin (Paris) ; 78(3): 269-277, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32540814

RESUMO

The SARS-CoV-2 virus is responsible for an epidemic disease called COVID-19, which was initially evidenced in Wuhan, China, and spread very rapidly in China and around the world. In France, the first isolated case seems now to be reported in December 2019, stage 3 of the COVID-19 epidemic was triggered on March 14th, the start of the planned containment exit from May 11th. Healthcare services have faced a large influx of patients who may be beyond their capacity to receive and care, particularly in the Large-East and Ile-de-France regions. Some patients show an evolution of the disease never observed before with other coronaviruses and develop in a few days a very important inflammatory reaction, which can lead to death of patients. A working group of the French Society of Clinical Biology (SFBC) was set up with the objective of providing updated information on the current status of the biological prescriptions (focusing on biochemistry ones) and their evolution during the epidemic, and of analyzing the biological parameters associated with comorbidities and patient evolution in order to link biological results with medical events. The expanded working group covers all sectors of medical biology in France and extends to the French-speaking world: hospital sectors (CHU and CH, Army Training Hospitals) and the private sector opening a field of view on the biological situation in establishments for dependent elderly, social establishments and clinical medical institutions. The purpose of this article is the presentation of this working group and its immediate and future actions.


Assuntos
Betacoronavirus , Bioquímica/organização & administração , Biomarcadores/análise , Serviços de Laboratório Clínico/organização & administração , Infecções por Coronavirus/diagnóstico , Pneumonia Viral/diagnóstico , Sociedades Científicas/organização & administração , Betacoronavirus/isolamento & purificação , Betacoronavirus/patogenicidade , Bioquímica/normas , Biomarcadores/sangue , Serviços de Laboratório Clínico/normas , Redes Comunitárias/organização & administração , Redes Comunitárias/normas , Redes Comunitárias/tendências , Infecções por Coronavirus/sangue , Infecções por Coronavirus/epidemiologia , Surtos de Doenças , França/epidemiologia , História do Século XXI , Humanos , Colaboração Intersetorial , Pandemias , Pneumonia Viral/sangue , Pneumonia Viral/epidemiologia , Prática Profissional/organização & administração , Prática Profissional/normas , Prática Profissional/tendências , Sociedades Científicas/normas , Comunicação por Videoconferência/organização & administração , Comunicação por Videoconferência/normas
9.
Klin Lab Diagn ; 65(3): 197-200, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32163696

RESUMO

In connection with the introduction of annexes and changes to the Federal State Educational Standard of Higher Education in the specialty 30.05.01 «Medical Biochemistry¼, a need arose to improve the assessment tool of general professional and professional competencies formation in the discipline - clinical laboratory diagnostics. A competence-oriented exam (COE) in the studied discipline is developed and implemented. Admission to the exam is the development of practical skills by students in the discipline modules, portfolio design and posting the results on the "Moodle" platform. COE includes two stages: testing to assess the level of theoretical knowledge deepening and solving a situational problem to assess the skills and abilities, interpret the results of laboratory tests and prepare a clinical and laboratory report. Situational tasks and checklists have been developed that make it possible to conduct an objective and comprehensive assessment of the competencies formed by students during the training process, which allows the graduate completed the program to solve professional problems.


Assuntos
Desempenho Acadêmico , Bioquímica/educação , Competência Clínica , Técnicas de Laboratório Clínico , Estudantes , Avaliação Educacional , Humanos
10.
Nat Commun ; 11(1): 793, 2020 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-32034152

RESUMO

Fluorescence-based technologies have revolutionized in vivo monitoring of biomolecules. However, significant technical hurdles in both probe chemistry and complex cellular environments have limited the accuracy of quantifying these biomolecules. Herein, we report a generalizable engineering strategy for dual-emission anti-Kasha-active fluorophores, which combine an integrated fluorescein with chromene (IFC) building block with donor-π-acceptor structural modification. These fluorophores exhibit an invariant near-infrared Kasha emission from the S1 state, while their anti-Kasha emission from the S2 state at around 520 nm can be finely regulated via a spirolactone open/closed switch. We introduce bio-recognition moieties to IFC structures, and demonstrate ratiometric quantification of cysteine and glutathione in living cells and animals, using the ratio (S2/S1) with the S1 emission as a reliable internal reference signal. This de novo strategy of tuning anti-Kasha-active properties expands the in vivo ratiometric quantification toolbox for highly accurate analysis in both basic life science research and clinical applications.


Assuntos
Bioquímica/métodos , Corantes Fluorescentes/química , Imagem Molecular/métodos , Células A549 , Animais , Benzopiranos/química , Cisteína/análise , Feminino , Fluoresceína/química , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/metabolismo , Glutationa/análise , Células Hep G2 , Humanos , Camundongos Endogâmicos BALB C , Estrutura Molecular , Neoplasias Experimentais/diagnóstico por imagem , Piranos/química , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Espironolactona/química
11.
Nat Commun ; 11(1): 784, 2020 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-32034159

RESUMO

Relatively robust dynamic covalent interactions have been employed extensively to mediate molecular self-assembly reactions; however, these assembly processes often do not converge to a thermodynamic equilibrium, instead yielding mixtures of kinetically-trapped species. Here, we report a dynamic covalent self-assembly process that mitigates kinetic trapping such that multiple unique oligomers bearing covalently coreactive pendant groups are able to undergo simultaneous, sequence-selective hybridization with their complementary strands to afford biomimetic, in-registry molecular ladders with covalent rungs. Analogous to the thermal cycling commonly employed for nucleic acid melting and annealing, this is achieved by raising and lowering the concentration of a multi-role reagent to effect quantitative dissociation and subsequently catalyze covalent bond rearrangement, affording selective assembly of the oligomeric sequences. The hybridization specificity afforded by this process further enabled information encoded in oligomers to be retrieved through selective hybridization with complementary, mass-labeled sequences.


Assuntos
Bioquímica/métodos , Peptoides/química , Aldeídos/química , Aminas/química , Fluoresceínas/química , Transferência Ressonante de Energia de Fluorescência , Iminas/química , Cinética , Hibridização de Ácido Nucleico , Peptoides/síntese química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , p-Dimetilaminoazobenzeno/análogos & derivados , p-Dimetilaminoazobenzeno/química
14.
J Biol Chem ; 295(3): 822-832, 2020 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-31953248

RESUMO

I have traveled many roads during my career. After spending my first 19 years in Los Angeles, I became somewhat of an academic nomad, studying and/or working in six universities in the United States and three in Sweden. In chronological order, I have a B.A. in Scandinavian languages and literature from UCLA, a Ph.D. in biochemistry from Uppsala University, and an M.S. in toxicology from the Karolinska Institute. I have been in schools of natural science, pharmacy, and medicine and have worked in multiple basic science departments and one clinical department. I have served as a research-track and tenured faculty member, department chair, associate dean, and dean. My research has spanned toxinology, biochemistry, toxicology, and pharmacology. Through all the moves, I have gained much and lost some. For the past 40 years, my interest has been cytochrome P450 structure-function and structure-activity relationships. My lab has focused on CYP2B enzymes using X-ray crystallography, site-directed mutagenesis, deuterium-exchange MS, isothermal titration calorimetry, and computational methods in conjunction with a variety of functional assays. This comprehensive approach has enabled detailed understanding of the structural basis of the remarkable substrate promiscuity of CYP2B enzymes. We also have investigated the mechanisms of CYP3A4 allostery using biophysical and advanced spectroscopic techniques, and discovered a pivotal role of P450-P450 interactions and of multiple-ligand binding. A major goal of this article is to provide lessons that may be useful to scientists in the early and middle stages of their careers and those more senior scientists contemplating an administrative move.


Assuntos
Bioquímica/educação , Escolha da Profissão , Relação Estrutura-Atividade , Cristalografia por Raios X , Sistema Enzimático do Citocromo P-450/química , Humanos , Idioma , Mutagênese Sítio-Dirigida , Suécia , Estados Unidos , Universidades
15.
Nat Protoc ; 15(3): 925-990, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31996842

RESUMO

Since the advent of modern science, researchers have had to rely on their technical skills or the support of specialized workshops to construct analytical instruments. The notion of the 'fourth industrial revolution' promotes construction of customized systems by individuals using widely available, inexpensive electronic modules. This protocol shows how chemists and biochemists can utilize a broad range of microcontroller boards (MCBs) and single-board computers (SBCs) to improve experimental designs and address scientific questions. We provide seven example procedures for laboratory routines that can be expedited by implementing this technology: (i) injection of microliter-volume liquid plugs into microscale capillaries for low-volume assays; (ii) transfer of liquid extract to a mass spectrometer; (iii) liquid-gas extraction of volatile organic compounds (called 'fizzy extraction'), followed by mass spectrometric detection; (iv) monitoring of experimental conditions over the Internet cloud in real time; (v) transfer of analytes to a mass spectrometer via a liquid microjunction interface, data acquisition, and data deposition into the Internet cloud; (vi) feedback control of a biochemical reaction; and (vii) optimization of sample flow rate in direct-infusion mass spectrometry. The protocol constitutes a primer for chemists and biochemists who would like to take advantage of MCBs and SBCs in daily experimentation. It is assumed that the readers have not attended any courses related to electronics or programming. Using the instructions provided in this protocol and the cited material, readers should be able to assemble simple systems to facilitate various procedures performed in chemical and biochemical laboratories in 1-2 d.


Assuntos
Bioquímica/instrumentação , Bioquímica/métodos , Computadores , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Software
16.
Biochemistry ; 59(1): 1-7, 2020 01 14.
Artigo em Inglês | MEDLINE | ID: mdl-31931576

Assuntos
Bioquímica , Animais , Ásia , Humanos
17.
Biochem Mol Biol Educ ; 48(1): 54-60, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31724817

RESUMO

One of the goals for requiring all college students to take science courses is to develop critical thinking about scientific issues they will encounter as citizens, consumers, and patients. This article integrates skill development activities targeting civic scientific literacy skills in a nonscience majors (liberal arts) biochemistry course, but similar principles could be integrated into nursing and major levels biochemistry courses. Strategies include teaching information acquisition, source analysis argument analysis, and civic engagement. These can be integrated into classroom activities, homework assignments, and outside of class projects. The impact of these activities was assessed through performance on the activities, open ended exam questions, and an end of course survey. Students gained both biochemical content and skills in evaluating claims with evidence, and reported the course helped them better understand what scientists do and how to make decisions based upon scientific evidence without detracting from the typical content learning goals of the course.


Assuntos
Bioquímica/educação , Currículo , Avaliação Educacional , Alfabetização , Ciência , Humanos , Estudantes , Pensamento , Universidades
18.
Biochem Mol Biol Educ ; 48(1): 74-79, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31532881

RESUMO

Enzyme kinetics is the study of enzymatic catalytic rates in biochemical reactions. This topic is commonly taught to life science students in introductory biochemistry courses during their undergraduate education. Unlike most other biochemistry topics, which focus on visual structures of biomolecules and their processes, enzyme kinetics is explained primarily through abstract mathematical and two-dimensional graphical plots. However, these abstract/symbolic representations often make it difficult for students to relate the kinetic parameters to the underlying molecular system that is being described. In this article, we present the design and development of a web-based multimedia interactive learning tool, biomolecular interactive tutorials (BIOMINT) to help students better bridge the relationships between these abstract mathematical models and the molecular behaviors, interactions, and dynamics that produce kinetic phenomena. This learning tool can be accessed at https://bit.ly/biomint. © 2019 International Union of Biochemistry and Molecular Biology, 48(1):74-79, 2020.


Assuntos
Bioquímica/educação , Enzimas/metabolismo , Aprendizagem , Projetos de Pesquisa , Estudantes , Disciplinas das Ciências Biológicas , Humanos , Cinética , Universidades
19.
Biochem Mol Biol Educ ; 48(1): 61-66, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31532885

RESUMO

Flow cytometry has become an important tool in the life sciences and medical fields, yet there are often few opportunities for undergraduate students to receive training with this type of instrumentation as part of life science curricula at many colleges and universities. We describe a straightforward laboratory exercise designed for a college-level biochemistry course that uses flow cytometry to investigate changes in bacterial membrane potential and cell viability in response to various treatments. Anecdotally, we have noticed that many students often have difficulty understanding the concept of membrane potential and the essential role that it plays in cellular processes. Therefore, this exercise also provides a pedagogical tool for visualizing changes in cellular membrane potential as way to enhancing students' comprehension of this abstract concept. Students' understanding of flow cytometry and membrane potential was assessed using a preactivity and postactivity quiz; the results indicate that the students' understanding of these concepts significantly improved after they completed this laboratory exercise. © 2019 International Union of Biochemistry and Molecular Biology, 48(1):61-66, 2020.


Assuntos
Membrana Externa Bacteriana/metabolismo , Bioquímica/educação , Currículo , Citometria de Fluxo , Laboratórios , Staphylococcus aureus/metabolismo , Sobrevivência Celular , Tecnologia Educacional , Humanos , Potenciais da Membrana , Staphylococcus aureus/citologia , Estudantes , Universidades
20.
Ann Rev Mar Sci ; 12: 23-48, 2020 01 03.
Artigo em Inglês | MEDLINE | ID: mdl-31433959

RESUMO

Biogeochemical-Argo (BGC-Argo) is a network of profiling floats carrying sensors that enable observation of as many as six essential biogeochemical and bio-optical variables: oxygen, nitrate, pH, chlorophyll a, suspended particles, and downwelling irradiance. This sensor network represents today's most promising strategy for collecting temporally and vertically resolved observations of biogeochemical properties throughout the ocean. All data are freely available within 24 hours of transmission. These data fill large gaps in ocean-observing systems and support three ambitions: gaining a better understanding of biogeochemical processes (e.g., the biological carbon pump and air-sea CO2 exchanges) and evaluating ongoing changes resulting from increasing anthropogenic pressure (e.g., acidification and deoxygenation); managing the ocean (e.g., improving the global carbon budget and developing sustainable fisheries); and carrying out exploration for potential discoveries. The BGC-Argo network has already delivered extensive high-quality global data sets that have resulted in unique scientific outcomes from regional to global scales. With the proposed expansion of BGC-Argo in the near future, this network has the potential to become a pivotal observation system that links satellite and ship-based observations in a transformative manner.


Assuntos
Monitoramento Ambiental/instrumentação , Tecnologia de Sensoriamento Remoto/métodos , Água do Mar/química , Bioquímica , Técnicas Biossensoriais/instrumentação , Clorofila A/análise , Concentração de Íons de Hidrogênio , Nitratos/análise , Oceanos e Mares , Oxigênio/análise
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