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1.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 479-485, 2019 Oct 12.
Artigo em Chinês | MEDLINE | ID: mdl-31713375

RESUMO

OBJECTIVE: To understand the prevalence and risk factors of Blastocystis hominis infection in inpatients in Jiangjin District, Chongqing City. METHODS: A cross-sectional study was conducted in a community hospital in Jiangjin District, Chongqing City, and the inpatients were surveyed by questionnaires. After obtaining the informed consent from the inpatients or legal guardians, the stool and blood samples were collected and examined by microscopy and PCR from April 17 to May 1, 2018. The univariate analysis and logistic regression analysis were used to analyze the risk factors of the B. hominis infection. RESULTS: A total of 198 hospitalized patients were investigated, and the infection rate of B. hominis was 10.61% (21/198), and the infection rate of the females (12.10%) was higher than that of the males (8.11%), but the difference was not statistically significant. The highest rate of infection was 19.23% in the age group of 10 to 20 years, followed by 17.74% in the age group of 60 years and above, and the lowest rate was 2.38% in the age group of 20 to 40 years. The difference in infection rates of B. hominis among the different age groups was statistically significant (P < 0.05). The infection rate of B. hominis in the people who used dry pail latrines was 33.30%, which was higher than that of the people who used water flush toilets (9.10%) (P < 0.05). The genotypes of B. hominis were ST1, ST3, ST6 and ST7, and ST6 and ST3 being the most predominant genotypes which accounted for 47.62% (10/21) and 38.10% (8/21) respectively, and among the infected males, the genotypes were only ST3 and ST6. The multiple logistic regression analysis showed that among the factors affecting B. hominis infection, only keeping pets was a risk factor [OR = 3.798, 95% CI (1.245, 11.581), P < 0.05]. CONCLUSIONS: A high prevalence of B. hominis infection is found in the inpatients in Jiangjin District, Chongqing City, the predominant genotypes are ST6 and ST3, and keeping pets may be one of the main risk factors.


Assuntos
Infecções por Blastocystis , Blastocystis hominis , Adolescente , Adulto , Infecções por Blastocystis/sangue , Infecções por Blastocystis/epidemiologia , Blastocystis hominis/genética , Criança , China/epidemiologia , Estudos Transversais , Fezes/parasitologia , Feminino , Hospitais/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Inquéritos e Questionários , Adulto Jovem
2.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 498-503, 2019 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-31713378

RESUMO

OBJECTIVE: To investigate the prevalence and risk factors of Blastocystis hominis infections among patients with HIV/AIDS in Fuyang City, Anhui Province. METHODS: A cross-sectional study was conducted in Fuyang City, Anhui Province in 2016. The demographic and socioeconomic status, and the lifestyle and production style were collected using a questionnaire survey. B. hominis DNA was detected in subjects'stool samples using a PCR assay, and the CD4+ T lymphocyte count and HIV viral load were measured in the subjects' blood samples. The risk factors of B. hominis infections among patients with HIV/AIDS were identified using univariate and multivariate logistic regression analyses. RESULTS: A total of 398 HIV/AIDS patients were enrolled in this study, with a mean age of 49.3 years, a mean body weight of 55.9 kg and a mean height of 164.4 cm. The prevalence of B. hominis infection was 6.78% in the study subjects, and no gender- (χ2 = 1.589, P = 0.207), education level- (χ2 =0.508, P = 0.776), marital status- (χ2 = 0.419, P = 0.811) or occupation-specific prevalence (χ2 = 2.744, P = 0.615) was detected. Among the patients with HIV/AIDS, there were no significant differences in the age (t = 0.370, P = 0.712), height (t = 1.587, P =0.113), body weight (t = 0.516, P = 0.606), CD4+ T lymphocyte count (t = 1.187, P = 0.230) or HIV viral load (t = 0.193, P =0.496) between B. hominis-infected and uninfected individuals. Dinking non-tap water [OR = 6.554, 95% CI: (1.876 to 22.903)] and keeping dogs [OR = 5.895, 95% CI: (2.017 to 17.225)] were identified as risk factors for B. hominis infection in patients with HIV/AIDS. CONCLUSIONS: The prevalence of B. hominis infection is high in HIV/AIDS patients, and drinking non-tap water and keeping dogs are risk factors for B. hominis infection among HIV/AIDS patients.


Assuntos
Infecções por Blastocystis , Blastocystis hominis , Infecções por HIV , Animais , Infecções por Blastocystis/complicações , Infecções por Blastocystis/epidemiologia , Estudos Transversais , Cães , Fezes/parasitologia , Feminino , HIV , Infecções por HIV/complicações , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco
3.
Comp Immunol Microbiol Infect Dis ; 65: 160-164, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31300108

RESUMO

BACKGROUND: Blastocystis, a common intestinal protozoan of humans and animals, infected more than 1 billion people around the world. This enteric protozoan is frequently reported in both healthy individuals and patients with gastrointestinal (GI) symptoms. METHODS: Three hundred and forty-five fecal samples including 151 GI patients and 194 healthy individuals were examined by microscopy, culture and PCR-sequencing techniques to determine Blastocystis frequency and subtype (ST) variation. RESULTS: The occurrence of Blastocystis was detected 56 (16.2%) and 85 (24.6%) by microscopy, culture and PCR methods, respectively. Out of the 85 positive patients, 60 (70.6%) were asymptomatic and 25 (29.4%) were symptomatic. The results of 41 successfully sequenced isolates identified 8 (19.5%), 8 (19.5%), and 25 (61.0%) ST1, ST2, and ST3, respectively. CONCLUSION: This study has found that Blastocystis was more common in healthy individuals than GI patients. Another finding was that no correlation was found between clinical symptoms and Blastocystis STs.


Assuntos
Infecções por Blastocystis/epidemiologia , Blastocystis hominis/isolamento & purificação , Gastroenteropatias/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Infecções Assintomáticas/epidemiologia , Blastocystis hominis/classificação , Blastocystis hominis/genética , Criança , Pré-Escolar , Estudos Transversais , DNA de Protozoário/genética , Fezes/parasitologia , Feminino , Gastroenteropatias/parasitologia , Variação Genética , Voluntários Saudáveis , Humanos , Lactente , Irã (Geográfico)/epidemiologia , Masculino , Pessoa de Meia-Idade , Filogenia , Prevalência , Adulto Jovem
4.
Acta Crystallogr D Struct Biol ; 75(Pt 7): 647-659, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31282474

RESUMO

Succinyl-CoA synthetase (SCS) catalyzes the only step of the tricarboxylic acid cycle that leads to substrate-level phosphorylation. Some forms of SCS are specific for ADP/ATP or for GDP/GTP, while others can bind all of these nucleotides, generally with different affinities. The theory of `gatekeeper' residues has been proposed to explain the nucleotide-specificity. Gatekeeper residues lie outside the binding site and create specific electrostatic interactions with incoming nucleotides to determine whether the nucleotides can enter the binding site. To test this theory, the crystal structure of the nucleotide-binding domain in complex with Mg2+-ADP was determined, as well as the structures of four proteins with single mutations, K46ßE, K114ßD, V113ßL and L227ßF, and one with two mutations, K46ßE/K114ßD. The crystal structures show that the enzyme is specific for ADP/ATP because of interactions between the nucleotide and the binding site. Nucleotide-specificity is provided by hydrogen-bonding interactions between the adenine base and Gln20ß, Gly111ß and Val113ß. The O atom of the side chain of Gln20ß interacts with N6 of ADP, while the side-chain N atom interacts with the carbonyl O atom of Gly111ß. It is the different conformations of the backbone at Gln20ß, of the side chain of Gln20ß and of the linker that make the enzyme ATP-specific. This linker connects the two subdomains of the ATP-grasp fold and interacts differently with adenine and guanine bases. The mutant proteins have similar conformations, although the L227ßF mutant shows structural changes that disrupt the binding site for the magnesium ion. Although the K46ßE/K114ßD double mutant of Blastocystis hominis SCS binds GTP better than ATP according to kinetic assays, only the complex with Mg2+-ADP was obtained.


Assuntos
Trifosfato de Adenosina/metabolismo , Blastocystis hominis/enzimologia , Modelos Moleculares , Succinato-CoA Ligases/química , Succinato-CoA Ligases/genética , Succinato-CoA Ligases/metabolismo , Sítios de Ligação , Cristalografia por Raios X/métodos , Escherichia coli/genética , Fluorometria/métodos , Ligações de Hidrogênio , Cinética , Mutação , Ligação Proteica , Domínios Proteicos
5.
J Parasitol ; 105(3): 480-483, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31251704

RESUMO

Stool ova and parasite examination using concentration methods remains the gold standard for the investigation of digestive parasitosis. Recently, single-use filtration devices have been marketed for stool concentration sedimentation methods such as MIF or Bailenger, which improve the analytical quality by avoiding contact with feces. In this study, the Parasep® device was adapted to the Faust technique flotation method. In addition, the performance between conventional techniques (MIF concentration and Faust) and techniques using this device was evaluated on 25 formalin-preserved stools and 3 fresh stools. With the Parasep device, the main parasites (protozoa or helminths) were isolated, and the technical requirements such as hygiene control for the operator and realization according to good laboratory practice were improved due to the filtration device.


Assuntos
Fezes/parasitologia , Parasitos/isolamento & purificação , Doenças Parasitárias/diagnóstico , Animais , Blastocystis hominis/isolamento & purificação , Diarreia/parasitologia , Entamoeba/isolamento & purificação , Giardia lamblia/isolamento & purificação , Doenças Parasitárias/parasitologia
6.
Turkiye Parazitol Derg ; 43(2): 70-73, 2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31204458

RESUMO

Objective: The aim of present study was to retrospectively determine the prevalence of intestinal parasites in patients, who were referred to Parasitology Laboratory in Van Yüzüncü Yil University, Faculty of Medicine during an 11-year period. Methods: Stool samples of 69633 individuals admitted to the outpatient clinics in the hospital were initially evaluated with native-Lugol, and then by flotation, sedimentation, trichrome staining, modified acid-fast staining and ELISA methods. Results: Twenty-four parasite species were identified in parasite-positive patients. At least one or more parasite species were found in 34.1% of all patients. The most commonly observed parasite was Blastocystis hominis (26.5%). Among pathogen parasites, Giardia intestinalis (G. intestinalis) was detected in 9.3%, Ascaris lumbricoides (A. lumbricoides) was detected in 2.5%, Entamoeba histolytica/Entamoeba dispar in 0.8%, Cystoisospora belli in 0.004%, Fasciola hepatica in 0.04%, Dicrocoelium dendriticum in 0.001%, Strongyloides stercoralis in 0.001% and hookworm in 0.001% of the patients. Conclusion: It was determined that, pathogen parasites such as G. intestinalis and A. lumbricoides were still observed at high rates in Van province, especially in children, and the problem of parasitosis is still continuing, although the prevalence of parasites has declined when compared to the previous years.


Assuntos
Helmintíase/epidemiologia , Enteropatias Parasitárias/epidemiologia , Infecções por Protozoários/epidemiologia , Adolescente , Animais , Ascaríase/epidemiologia , Ascaris lumbricoides/isolamento & purificação , Infecções por Blastocystis/epidemiologia , Blastocystis hominis/isolamento & purificação , Criança , Dicrocelíase/epidemiologia , Dicrocoelium/isolamento & purificação , Entamoeba/classificação , Entamoeba/isolamento & purificação , Entamebíase/epidemiologia , Fasciola hepatica/isolamento & purificação , Fezes/parasitologia , Feminino , Giardia lamblia/isolamento & purificação , Giardíase/epidemiologia , Humanos , Masculino , Parasitos , Prevalência , Estudos Retrospectivos , Strongyloides stercoralis/isolamento & purificação , Turquia/epidemiologia
7.
Epidemiol Health ; 41: e2019009, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30999737

RESUMO

OBJECTIVES: Blastocystis hominis is a very common large intestinal protozoan with global prevalence in humans and non-human hosts. No precise statistics exist regarding the geographical distribution of Blastocystis that would enable the identification of high-risk communities. Therefore, the current research aimed to characterize the spatial patterns and demographic factors associated with B. hominis occurrence in northern Iran. METHODS: The current study was performed among 4,788 individuals referred to health centers in Mazandaran Province, from whom stool samples were obtained. Socio-demographic data were gathered using a questionnaire. Samples were examined by a direct wet mount, the formalin-ethyl acetate concentration technique, and trichrome staining. Moran local indicators of spatial association and a geographically weighted regression model were utilized to analyze the results. RESULTS: Generally, the infection rate of Blastocystis parasites was 5.2%, and was considerably higher in the age group of 10-14 years (10.6%) than in other age groups (p=0.005). Our data showed important associations between the occurrence of B. hominis and age, residence, job, contact with domestic animals, anti-parasitic drug consumption, and elevation above sea level (p<0.001). CONCLUSIONS: The current study characterized for the first time the infection rate and risk of B. hominis in the north of Iran, and produced a prediction map. It is expected that this map will help policymakers to plan and implement preventive measures in high-risk areas and to manage already-infected patients.


Assuntos
Infecções por Blastocystis/epidemiologia , Blastocystis hominis/isolamento & purificação , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Estudos Transversais , Fezes/parasitologia , Feminino , Sistemas de Informação Geográfica , Humanos , Lactente , Irã (Geográfico)/epidemiologia , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Análise Espacial , Inquéritos e Questionários , Adulto Jovem
8.
Turkiye Parazitol Derg ; 43(1): 16-20, 2019 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-30938126

RESUMO

Objective: Food handlers play an important role in the production and distribution of parasitic contaminations in these societies. The aim of this study was to assess the prevalence of intestinal parasitic infections among the food handlers in Qazvin, Iran. Methods: Totally, 1530 stool samples were collected from food handlers in Qazvin, Iran. Direct feces examination and formalinethyl acetate concentration method followed by Trichrome staining were performed to detect the parasites. Results: The prevalence of intestinal parasites was 82 (5.4%) among food handlers. The species of protozoan parasites found were Blastocystis hominis, Giardia lamblia, Entamoeba coli, and Endolimax nana. The outbreaks of Blastocystis hominis (2.9%), and Endolimax nana (0.3%) were the most and the least protozoan infections observed among the specimens, respectively. Also, there was no helminthic infection detected among the samples. The prevalence of intestinal parasites among the food handlers, based on variables including sex, age, job, and education, did not differ, whereas the prevalence of intestinal symptoms was shown to be higher in infected food handlers (p<0.05). Conclusions: Despite a decrease in the incidence of intestinal parasites, the protozoan infections are still among the health concerns in these areas. Therefore, continuous monitoring and training of food handlers by local health authorities should be performed on a regular basis.


Assuntos
Manipulação de Alimentos , Enteropatias Parasitárias/epidemiologia , Adulto , Infecções por Blastocystis/epidemiologia , Blastocystis hominis/isolamento & purificação , Fezes/parasitologia , Feminino , Giardia lamblia/isolamento & purificação , Giardíase/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Turquia/epidemiologia
9.
Trop Doct ; 49(3): 184-188, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30885054

RESUMO

The pathogenesis of irritable bowel syndrome (IBS) is not yet clear. Our study suggested parasitic infection and other plausible risk factors among Egyptian IBS patients. We studied 40 IBS patients diagnosed according to Rome III criteria (Group I), 40 with other gastrointestinal symptoms (Group II) and 40 healthy controls (Group III). Stool samples were examined using direct wet smear, sedimentation technique, trichrome stain and immune-chromatographic tests for Cryptosporidium parvum. IBS patients displayed a significantly greater percentage of Blastocystis hominis infection (45%) than non-IBS patients (20%) and healthy controls (10%). Dientamoeba fragilis was identified in two IBS patients. Detection of B. hominis was independent of demographic characters, IBS subtype, Helicobacter pylori infection or medications, but with a positive association with a history of antibiotic intake with IBS.


Assuntos
Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Síndrome do Intestino Irritável/epidemiologia , Síndrome do Intestino Irritável/parasitologia , Adolescente , Adulto , Blastocystis hominis/isolamento & purificação , Dientamoeba/isolamento & purificação , Egito/epidemiologia , Fezes/parasitologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Adulto Jovem
10.
Turk J Gastroenterol ; 30(1): 40-46, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30644363

RESUMO

BACKGROUND/AIMS: Several studies have shown that a change in microbiota plays an important role in the pathogenesis of inflammatory bowel disease (IBD). Furthermore, with the emergence in recent studies of differences according to the subtype of IBD and whether the disease is active or in remission, there has started to be research into the relationship between IBD and several microorganisms. Blastocystis hominis is primary among these organisms. The aim of the present study was to determine the role of B. hominis in the acute flare-up of ulcerative colitis (UC). MATERIALS AND METHODS: A total of 114 patients with UC were included in the study, with 52 in the active phase. The Mayo scoring system was used for the activity index. Patients determined with a flare-up agent other than B. hominis were excluded from the study. Fecal samples of the patients were examined by the polymerase chain reaction method for the presence of B. hominis. RESULTS: B. hominis positivity was determined in 37 (34%) patients with UC. Of the patients, 17 (32.6%) were in the acute flare-up phase, and 20 (32.2%) were in remission (p=0.961). In 11 (64.7%) of the B. hominis positive patients, the disease severity was determined as mild-moderate (p<0.001). CONCLUSION: The results of the present study showed that while there was no difference between the active and remission phases in respect of B. hominis presence, there was milder involvement in those determined with B. hominis.


Assuntos
Infecções por Blastocystis/complicações , Blastocystis hominis , Colite Ulcerativa/parasitologia , Exacerbação dos Sintomas , Adulto , Fezes/parasitologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Indução de Remissão
11.
Acta Parasitol ; 63(4): 819-825, 2018 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-30367755

RESUMO

The aim of the research was to determine the prevalence of non-pathogenic protozoa circulating in the human population of Slovakia. We particularly focused on the socially deprived areas with poor sanitation conditions, as they are one of the factors affecting the transmission of these infections. Within this study, 2760 people were coprologically screened for the presence of protozoan cysts. The analyzed group comprised 1173 men and 1587 women from different regions of Slovakia. The total prevalence (2.03%) of non-pathogenic protozoa species was determined. The prevalence of Entamoeba coli was 0.80%, the prevalence of Endolimax nana 0.58%, and the prevalence of Blastocystis hominis was 0.65%. The presence of non-pathogenic protozoa was more frequent in women than that in men, in all age groups. The highest incidence of Entamoeba coli was found in children aged one month - seven years (0.79%), the lowest in the age group of 19-88 years (0.66%). Endolimax nana was most frequent in 8-18 year-olds (0.95%), where the statistical significance was found (p<0.05). The prevalence of Blastocystis hominis by the age group ranged from 0.39 to 0.95%. We did not find any statistical significance (p>0.05) for Entamoeba coli, and similarly for Blastocystis hominis associated with the sex and age. Although the circulation of non-pathogenic protozoa in the human population is far from being limited to the developing countries, their occurrence is also frequent in the population of developed countries. Despite their controversial pathogenicity, they should not be neglected, particularly in the patients with gastrointestinal symptoms.


Assuntos
Gastroenteropatias/epidemiologia , Enteropatias Parasitárias/epidemiologia , Infecções por Protozoários/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Amebíase/epidemiologia , Amebíase/parasitologia , Infecções por Blastocystis/epidemiologia , Infecções por Blastocystis/parasitologia , Blastocystis hominis/isolamento & purificação , Criança , Pré-Escolar , Endolimax/isolamento & purificação , Entamoeba/isolamento & purificação , Entamebíase/epidemiologia , Entamebíase/parasitologia , Fezes/parasitologia , Feminino , Gastroenteropatias/parasitologia , Humanos , Incidência , Lactente , Enteropatias Parasitárias/parasitologia , Masculino , Pessoa de Meia-Idade , Prevalência , Infecções por Protozoários/parasitologia , Distribuição por Sexo , Eslováquia/epidemiologia , Adulto Jovem
12.
Turkiye Parazitol Derg ; 42(2): 113-117, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30070641

RESUMO

OBJECTIVE: The purpose of the present study was to investigate the distribution of intestinal parasites in patients hospitalized in a child intensive care unit. METHODS: The study was conducted on 150 patients between the ages of 2 months and 17 years between April 2016 and December 2016. Stool samples were tested by Native-Lugol, sedimentation (formol-ether solution), and acid fast method. Moreover, a questionnaire was used to obtain information about the socio-economic status of the patient, education level of the families, living conditions, and whether or not they have chronic illnesses. RESULTS: 40% of all patients were infected with one or more parasites 41.7% of girls and 38.2% of boys. In the present study, Giardia intestinalis (12.6%), Blastocystis hominis (12.6%), Entamoeba coli (7.3%), Cyclospora cayetanensis (5.3%), and Cryptosporidium spp. (2%) were detected. CONCLUSION: Statistical analysis was evaluated with the frequency of parasitism and revenue level, educational level of families, settlement area, whether there is a sewage network or not, whether animal husbandry is done or not, and source of water in home is from tap or well. The differences were not significantly. The possible reasons for the high rate of parasitosis, especially with G. intestinalis, E. coli, and B. hominis, are inadequate education, living in small houses with large numbers of people, sewage system, and lack of clean and safe drinking water.


Assuntos
Enteropatias Parasitárias/epidemiologia , Adolescente , Animais , Blastocystis hominis/isolamento & purificação , Criança , Pré-Escolar , Cryptosporidium/isolamento & purificação , Cyclospora/isolamento & purificação , Entamoeba/isolamento & purificação , Fezes/parasitologia , Feminino , Giardia lamblia/isolamento & purificação , Hospitalização , Humanos , Lactente , Unidades de Terapia Intensiva Pediátrica , Enteropatias Parasitárias/parasitologia , Masculino , Classe Social , Inquéritos e Questionários , Turquia/epidemiologia
14.
Acta Trop ; 185: 193-203, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29802845

RESUMO

Blastocystis is a prevalent eukaryotic microorganism that has been frequently reported from the stool samples of human and animals, all over the world. The prevalence of this parasite has close correlation with the climate and hygiene situation of studied populations. For the current study, English and Persian databases were searched for the following terms: "Blastocystis hominis", "Blastocystis sp.", "Intestinal Parasites", "Prevalence" and "Iran". In order to data analysis and assess the possibility of publication bias, STATA v11 and Begg's Funnel plot were employed respectively. From 605 searched cases, 86 publications met our criteria for the analysis. The pooled prevalence of Blastocystis was 9.1% (95%CI: 8.2%, 10.1%). The results represented that the prevalence of the parasite using molecular techniques or cultivation followed by molecular techniques (20.89%) was higher than only microscopical examination (8.96%). The prevalence rate of Blastocystis according to human development index was 7.6%, 7.2%, 11.9%, 13.4%, and 3.3% for ranks 1 to 5, respectively. This is the first study signifying the prevalence of Blastocystis regarding the human development index and climate conditions. The findings represented that although human development index, low levels of socioeconomic situations together with appropriate climate such as sufficient humidity and high temperature increase the chance of transmission of Blastocystis in a society.


Assuntos
Infecções por Blastocystis/epidemiologia , Blastocystis hominis , Clima , Desenvolvimento Humano , Animais , Fezes/parasitologia , Humanos , Irã (Geográfico)/epidemiologia , Prevalência
15.
Pesqui. vet. bras ; 38(5): 847-851, May 2018. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-955422

RESUMO

The health monitoring and management systems of coturniculture can be deemed to be in a developmental phase when compared to the poultry industry. Studies regarding taxonomy and parasitic biology in quails (Coturnix japonica) has not been well conducted in Brazil. Most of the information is available from the autopsy case reports, in many ways the parasitic fauna of quails is still unknown. The aim of this study was to conduct a parasitological research in quails in order to contribute to ameliorate this situation. 31 quails, which were 12 months old, were used for the study. Their carcasses and viscera were sent to the Laboratory of Entomology and Tropical Diseases, INPA, Manaus/AM. The circulatory, nervous, respiratory, digestive and reproductive systems of these were studied separately. No blood parasites were found, however, nine species of endoparasites were registered which were distributed among the classes Cestoda, Nematoda and Protozoa. The helminths were distributed in the duodenum, jejunum, ileum, cecum and oviduct. The cecum was found to be the most parasitized organ and contained a wide range of parasites having three species of protozoa and three species of nematodes. Six morphotypes of Eutrichomastix globosus were recorded, and some morphotypes were hyperparasitized with sporangia Sphaerita sp. in the cytoplasm. A large number of parasites were recorded in this study, as well as the protozoan Blastocystis hominis was first being observed for quail.(AU)


A coturnicultura conta com um monitoramento sanitário e sistemas de manejo ainda em desenvolvimento quando comparado à avicultura industrial. Pesquisas de taxonomia e biologia parasitárias em codornas (Coturnix japonica) são pouco realizadas no Brasil, sendo a maioria das informações disponíveis referentes a relatos de caso em achados de necropsia, portanto, em muitos aspectos a fauna parasitária de codornas é ainda desconhecida. Este trabalho teve por objetivo realizar uma pesquisa parasitológica em codornas em fim de postura. Para pesquisa foram disponibilizadas 31 codornas com idades de 12 meses. As carcaças e suas vísceras foram encaminhadas ao Laboratório de Entomologia e Doenças Tropicais INPA, Manaus/AM. Foram estudados separadamente os sistemas circulatórios, nervoso, respiratório, digestivo e reprodutivo. Das 31 codornas examinadas nenhuma apresentou hemoparasitos, contudo, foram registradas nove espécies de endoparasitos distribuídas entre as classes Cestoda, Nematoda e protozoários. Os helmintos distribuíam-se pelo duodeno, jejuno, íleo, cecos e oviduto. O ceco foi o órgão mais parasitado e com maior diversidade de parasitas, sendo três espécies de protozoários e três de nematóides. Foram registrados seis morfotipos de Eutrichomastix globosus, sendo que, alguns morfótipos estavam hiperparasitados com esporângio Sphaerita sp. no citoplasma. Uma grande variedade de parasitos foi registrada nesta pesquisa, bem como, o protozoário Blastocystis hominis pela primeira vez sendo descrito para codornas.(AU)


Assuntos
Animais , Blastocystis hominis/patogenicidade , Coturnix/parasitologia , Parabasalídeos/patogenicidade , Fazendas/organização & administração
16.
Rev. clín. esp. (Ed. impr.) ; 218(3): 115-120, abr. 2018. tab
Artigo em Espanhol | IBECS | ID: ibc-174240

RESUMO

Objetivos. Blastocystis hominis (B. hominis) es uno de los parásitos intestinales más frecuentemente aislados en el ser humano. Puede producir sintomatología gastrointestinal o, en la mayoría de los casos, permanecer asintomático. Existen dudas sobre el carácter patógeno del parásito. El objetivo de este estudio fue analizar las características clínicas y epidemiológicas de la parasitación por B. hominis, con y sin otras coparasitaciones. Pacientes y métodos. Estudio observacional retrospectivo de aislamientos de B. hominis en heces, desde octubre del 2004 hasta marzo del 2016 en una Unidad de Medicina Tropical. Se revisó a todos los pacientes con parasitación exclusiva, o no, por B. hominis. Resultados. Se estudió a 3.070 pacientes. En 570 (18%) se diagnosticó infección por B. hominis, de los que en 245 (43%) representó el único aislamiento; 325 (57%) presentaron otras coparasitaciones (Entamoeba hystolitica o dispar, Strongyloides stercoralis, uncinarias y Schistosoma sp.). El síntoma principal fue dolor abdominal (41,8%). En un 31,2% el parásito se detectó en el cribado de enfermedades importadas en pacientes asintomáticos. De los que recibieron tratamiento con metronidazol, un 78,2% mejoró y en el 82,6% los parásitos se negativizaron. Conclusiones. La parasitación por B. hominis es una de las enfermedades más frecuentes en nuestra Unidad de Medicina Tropical. La mayoría de los pacientes están asintomáticos o bien la clínica puede ser atribuida a otras parasitaciones. En aquellos casos en los que persisten los síntomas sin poder ser atribuidos a otras causas, es recomendable un tratamiento específico


Objectives. Blastocystis hominis (B. hominis) is one of the most common intestinal parasites isolated in humans. The parasite can cause gastrointestinal symptoms or, in most cases, remain asymptomatic. There are issues concerning the parasite's pathogenic character. The aim of this study was to analyse the clinical and epidemiological characteristics of the parasite infection by B. hominis, with or without other parasitic co-infections. Patients and methods. An observational retrospective study was conducted of B. hominis isolates in faeces from October 2004 to March 2016 in a tropical medicine unit. We reviewed all patients with a parasite infection, exclusively or not by B. hominis. Results. We studied 3070 patients, 570 (18%) of whom were diagnosed with B. hominis infection, which was the only isolate in 245 (43%) of the 570 patients. A total of 325 (57%) patients presented other parasitic co-infections (Entamoeba histolytic or Entamoeba dispar, Strongyloides stercoralis, hookworm and Schistosoma spp.). The main symptom was abdominal pain (41.8%). In 31.2% of cases, the parasite was detected in the imported diseases screening of asymptomatic patients. Of those who underwent treatment with metronidazole, 78.2% improved. The parasite was neutralised in 82.6% of the patients. Conclusions. Parasite infection by B. hominis is one of the most common diseases in our tropical medicine unit. Most patients are asymptomatic, or their symptoms can be attributed to other parasite infections. In those cases in which symptoms persist without being able to attribute them to other causes, a specific treatment is recommended


Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Enteropatias Parasitárias/epidemiologia , Infecções por Blastocystis/epidemiologia , Blastocystis hominis/isolamento & purificação , Metronidazol/uso terapêutico , Dor Abdominal/etiologia , Combinação Trimetoprima e Sulfametoxazol/uso terapêutico , Estudos Retrospectivos , Enteropatias Parasitárias/tratamento farmacológico , Anamnese/métodos , Enteropatias Parasitárias/urina , Esquistossomose/urina , Microfilárias , Microfilárias/parasitologia , Espanha/epidemiologia , Fezes/parasitologia
18.
New Microbiol ; 41(2): 173-177, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29498738

RESUMO

In the reported case, a 41-year-old Italian man came to the clinician's observation reporting cramps, bloating and watery diarrhoea a few days after drinking water indicated as unpotable from a fountain in a farm area. The medical suspicion was directed at both gluten intolerance and enteric infection, eventually of waterborne origin. Gluten intolerance was investigated by intestinal biopsy and excluded, while stool analyses ruled out infective bacteriological or viral agents and parasites. Subsequently, a persistent eosinophilia was revealed and a parasitological analysis was again suggested, planning for a more sensitive molecular method. Therefore, a multiplex-PCR of enteric protozoa species DNA was performed on an intestinal biopsy and faecal samples revealing only Blastocystis hominis protozoa, subsequently typed as subtype 1 by RFLP-PCR method. B. hominis is an anaerobic protozoa found in the human and animal intestinal tract, recently associated with a pathogenic role characterized by chronic development. Since blastocystosis has been demonstrated as a waterborne infection, a sample of water matrix was analysed, revealing the B. hominis subtype 1 DNA inside. A probable water transmission of Blastocystis infection has been demonstrated in this case report. Only a probiotic treatment based on Saccharomyces boulardii was administered to the patient and this apparently resolved the infection. In summary, the case described here is a chronic blastocystosis of possible waterborne origin, controlled by assuming a yeast treatment.


Assuntos
Infecções por Blastocystis/parasitologia , Blastocystis hominis/isolamento & purificação , Água/parasitologia , Adulto , Infecções por Blastocystis/patologia , Infecções por Blastocystis/terapia , Infecções por Blastocystis/transmissão , Humanos , Itália , Masculino , Probióticos/uso terapêutico
19.
Clin Microbiol Infect ; 24(12): 1333-1337, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29581055

RESUMO

OBJECTIVES: Multiplex PCR assays offer highly sensitive and specific tools for the detection of enteric pathogens. This prospective study aimed at comparing the novel Roche LightMix Modular Assay Gastro Parasites (LMAGP) detecting Giardia duodenalis, Entamoeba histolytica, Cryptosporidium spp., Blastocystis hominis, and Dientamoeba fragilis with routine laboratory procedures. METHODS: Stool specimens (n = 1062 from 1009 patients) were consecutively examined by LMAGP, R-Biopharm Ridascreen enzyme immunoassays (EIAs) detecting G. duodenalis or E. histolytica/dispar, and microscopy of wet mounts. Discrepant results were analysed by in-house PCR. RESULTS: D. fragilis or B. hominis were detected by LMAGP in 131 (14.4%) and 179 (19.9%; 16 samples positive by microscopy; p < 0.0001) of 909 samples, respectively. Of 918 samples analysed for Cryptosporidium spp., six were positive by LMAGP (three could be confirmed by Kinyoun staining and one by in-house PCR). G. duodenalis was detected by LMAGP, EIA, or microscopy in 20, 16, or 9 of 1039 stool samples, respectively; all four samples missed by EIA were confirmed by in-house PCR. In total, 938 stool samples were analysed for E. histolytica/dispar. Nine of ten EIA-positive samples were negative by LMAGP but positive by in-house PCR for E. dispar. One E. histolytica infection (positive by both LMAGP and in-house PCR) was missed by EIA and microscopy. Parasites only detected by microscopy included Enterobius vermicularis eggs (n = 3) and apathogenic amoebae (n = 27). CONCLUSIONS: The data call for routine use of multiplex PCR assays for the detection of enteric protozoan parasites in laboratory diagnostics.


Assuntos
Blastocystis hominis/genética , Cryptosporidium/genética , Dientamoeba/genética , Entamoeba histolytica/genética , Giardia lamblia/genética , Enteropatias Parasitárias/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Blastocystis hominis/isolamento & purificação , Criança , Pré-Escolar , Técnicas de Laboratório Clínico/métodos , Cryptosporidium/isolamento & purificação , Dientamoeba/isolamento & purificação , Entamoeba histolytica/isolamento & purificação , Fezes/parasitologia , Feminino , Giardia lamblia/isolamento & purificação , Humanos , Técnicas Imunoenzimáticas/métodos , Lactente , Recém-Nascido , Enteropatias Parasitárias/parasitologia , Masculino , Microscopia/métodos , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Multiplex/métodos , Estudos Prospectivos , Kit de Reagentes para Diagnóstico/estatística & dados numéricos , Sensibilidade e Especificidade , Adulto Jovem
20.
Am J Trop Med Hyg ; 98(3): 763-767, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29363443

RESUMO

Intestinal parasitic infection rate among school-aged children in Thailand has been decreasing. However, certain intestinal parasites remain problematic in some regions. This cross-sectional study was conducted between February and September 2016 in three suburban government primary schools (KK, BR, and HK), Saraburi, Thailand. Stool was collected from 263 asymptomatic subjects (4-15 years old), using simple direct smear, formalin-ether concentration, Boeck and Drbohlav's Locke-Egg-Serum (LES) medium culture, and agar plate culture. A self-administered questionnaire was used to collect data about lifestyle and socioeconomic status. The overall rate of intestinal parasites was 22.1% (15.6% single infection and 6.5% multiple infections). The helminths involving the digestive system found were Strongyloides stercoralis (1.5%) and Opisthorchis viverrini (0.4%). For protozoan infection, the major cause was Blastocystis hominis (17.5%). The other protozoa included Endolimax nana (4.6%), Entamoeba coli (3.4%), Entamoeba histolytica/Entamoeba dispar (1.1%), and Giardia intestinalis (0.8%). The sensitivity for the detection of B. hominis increased with the LES culture technique. The infection rate of each organism was not significantly different among the three schools except for B. hominis which showed the highest prevalence in the HK school (P = 0.001). This was correlated with the questionnaire results in which the HK school showed the highest risk of drinking contaminated water (P = 0.004). The present study emphasized the persistent problems of protozoan infections among suburban school-aged children. Lifestyle was still an important factor for intestinal parasitic infections among suburban school-aged Thai children in this study. Health education as well as routine surveillance was necessary to control the infections.


Assuntos
Amebíase/epidemiologia , Infecções por Blastocystis/epidemiologia , Entamebíase/epidemiologia , Opistorquíase/epidemiologia , Estrongiloidíase/epidemiologia , Adolescente , Amebíase/diagnóstico , Amebíase/parasitologia , Animais , Infecções por Blastocystis/diagnóstico , Infecções por Blastocystis/parasitologia , Blastocystis hominis/isolamento & purificação , Blastocystis hominis/patogenicidade , Criança , Pré-Escolar , Estudos Transversais , Endolimax/isolamento & purificação , Endolimax/patogenicidade , Entamoeba/isolamento & purificação , Entamoeba/patogenicidade , Entamebíase/diagnóstico , Entamebíase/parasitologia , Fezes/parasitologia , Feminino , Humanos , Masculino , Opistorquíase/diagnóstico , Opistorquíase/parasitologia , Opisthorchis/isolamento & purificação , Opisthorchis/patogenicidade , Classe Social , Strongyloides stercoralis/isolamento & purificação , Strongyloides stercoralis/patogenicidade , Estrongiloidíase/diagnóstico , Estrongiloidíase/parasitologia , Inquéritos e Questionários , Tailândia/epidemiologia
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