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1.
Food Chem ; 308: 125607, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31677599

RESUMO

Lipid sources as alternatives to fish oil could alter the nutritional value and flavor quality of crab meat affecting consumer preferences. Herein, an 8-week nutritional trial was designed to investigate the effects of dietary lipid sources including fish oil (FO), krill oil (KO), palm oil, rapeseed oil, soybean oil and linseed oil on profiles of amino acids, fatty acids and volatiles in muscle of swimming crab (Portunus trituberculatus). Volatiles of crab muscle were characterized by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry. Results revealed that crabs fed FO and KO had significantly higher levels of protein, indispensable amino acids, eicosapentaenoic acid and docosahexaenoic acid in muscle. Principal component analysis and hierarchical cluster analysis demonstrated that muscle volatiles of crabs fed different dietary oils exhibited significant variations. Dietary FO and KO significantly increased the relative levels of 3-methylbutanal, heptanal, benzaldehyde and nonanal in muscle, which may produce more pleasant flavors.


Assuntos
Braquiúros/química , Aromatizantes/análise , Animais , Gorduras na Dieta/análise , Ácidos Docosa-Hexaenoicos/análise , Ácido Eicosapentaenoico/análogos & derivados , Ácido Eicosapentaenoico/análise , Ácidos Graxos/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Estado Nutricional , Valor Nutritivo , Alimentos Marinhos/análise , Microextração em Fase Sólida , Natação , Paladar
2.
J Sci Food Agric ; 100(1): 384-393, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31595518

RESUMO

BACKGROUND: Seafood processing generates significant amounts of solid and liquid waste in the environment. Such waste represents a potential source of high-value biomolecules for food, pharmaceutic and cosmetic applications. There are very few studies on the valorization of wastewaters compared to solid by-products. However, cooking waters are characterized by a high organic polluting load, which could contain valuable molecules such as proteins, pigments and flavor compounds. Snow crab (Chionoecetes opilio) processing is included among the most important processes in Canadian fisheries, although its cooking effluent composition is not well characterized. RESULTS: The present study concentrated and valorized the biomass in snow crab cooking wastewaters for the development of products for food applications. A membrane process was designed and optimized to concentrate the effluents. The chemical composition of the concentrates was analyzed, including characterizing the flavor profile compounds. The extracts were mainly composed of proteins (592 g kg-1 ) and minerals (386 g kg-1 ) and contained desirable flavor compounds. Their functional properties (solubility, water-holding capacity, oil-holding capacity) and antioxidant activities were also assessed, and their safety was verified. CONCLUSION: The cooking effluents generated by snow crab processing facilities, usually considered as waste, can be concentrated and turned into a natural aroma for the food industry. © 2019 Society of Chemical Industry.


Assuntos
Proteínas de Artrópodes/análise , Braquiúros/química , Resíduos/análise , Águas Residuárias/química , Animais , Proteínas de Artrópodes/isolamento & purificação , Culinária , Indústria de Processamento de Alimentos
3.
Food Chem ; 302: 125334, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31419773

RESUMO

Carotenoids, natural pigments, are a group of chemically heterogeneous molecules, present in numerous taxonomical clusters. Because of their various bioactivities, carotenoids are day-by-day applied in numerous fields. The present work aimed to investigate an efficient extraction process of carotenoids from blue crab shells and their identification by HR-ESI-MS technique. In this context, different methods (enzymatic, maceration, Soxhlet, etc.) and solvents (variable polarity index) were tested. Maceration using the binary system hexane/isopropanol (50/50) was found to be the most efficient process, producing high carotenoids content and low total phenolic and soluble protein amounts (p < 0.05). When combined with an enzymatic pretreatment, this procedure was found to be remarkably (p < 0.05) more efficient and selective especially towards astaxanthin (p < 0.05). The HR-ESI-MS identified 23 compounds, depending on the adopted extraction approach. The compounds identified may have potential for applications in food or pharmaceutical industries.


Assuntos
Exoesqueleto/química , Braquiúros/química , Carotenoides/isolamento & purificação , Fracionamento Químico/métodos , 2-Propanol/química , Animais , Carotenoides/análise , Hexanos/química , Fenóis/análise , Fenóis/química , Proteínas/análise , Proteínas/isolamento & purificação , Solventes/química , Espectrometria de Massas por Ionização por Electrospray , Espectroscopia de Infravermelho com Transformada de Fourier , Xantofilas/análise , Xantofilas/isolamento & purificação
4.
J Agric Food Chem ; 67(46): 12918-12926, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31668066

RESUMO

The triosephosphate isomerase (TIM), Scy p 8, is a crab allergen and shows cross-reactivity in the shellfish. Here, recombinant Scy p 8 was expressed, and its crystal structure was determined at a resolution of 1.8 Å. The three-dimensional structure of Scy p 8 is primarily composed of a (ß/α)8-barrel motif prototype. Additionally, Scy p 8 showed cross-reactivity with high sequential and secondary structural identity among TIMs from shellfish species. The site-directed mutagenesis of critical amino acids of conformational epitopes was carried out, and the mutants of Trp 168 and Lys 237 to Ala reduced immunoglobulin E (IgE)-binding activity by approximately 30%, compared with wild-type TIM in an inhibition ELISA; however, it still induced basophil activation despite the interpatient variability between patients. These results can help to provide an accurate template for the analysis of the IgE binding and establish meaningful relationships between structure and allergenicity.


Assuntos
Braquiúros/enzimologia , Epitopos/química , Triose-Fosfato Isomerase/imunologia , Sequência de Aminoácidos , Animais , Braquiúros/química , Braquiúros/genética , Braquiúros/imunologia , Reações Cruzadas , Cristalização , Epitopos/genética , Epitopos/imunologia , Humanos , Imunoglobulina E/imunologia , Conformação Molecular , Conformação Proteica , Alinhamento de Sequência , Frutos do Mar/análise , Hipersensibilidade a Frutos do Mar/imunologia , Triose-Fosfato Isomerase/química , Triose-Fosfato Isomerase/genética
5.
J Food Sci ; 84(12): 3594-3600, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31762029

RESUMO

The effects of four different extraction methods (Folch, Soxhlet, two-step, and enzyme-assisted aqueous extraction) on the yields, lipid class, fatty acids (FAs) composition, minor components (including carotenoid, cholesterol), and thiobarbituric acid reactive substances values of lipids in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis) were investigated. The C16:0, C18:1, and C18:2 were identified to be the dominant FAs in crab lipids, and the FAs were present in the form of triglycerides. The Soxhlet and enzyme-assisted extraction were more suitable for crab lipids extraction, showing higher extraction rates and oxidative stability. Especially, the lipid extracted by enzyme-assisted extraction has high carotenoids content. The components of crab lipids extracted by enzyme-assisted aqueous extraction were further identified using untargeted metabolomics methods. The polyunsaturated fatty acid, sterols, amino acids, products of lipid ß-oxidation and ATP degradation, phosphatidyl ethanolamine, and astaxanthin were founded in crab oil. PRACTICAL APPLICATION: The Chinese mitten crab (Eriocheir sinensis) is a popular aquatic food in China. The hepatopancreas is the major lipid storage organ of crab, and the distinctive flavor of crab is mainly from it. To compare the different extraction methods on yield, composition and properties of crab lipids can be helpful for lipids production from crab hepatopancreas. Meanwhile, the crab hepatopancreas lipids are rich in polyunsaturated fatty acids and astaxanthin, and have potential to be as a functional component and a crab flavor additive in food industry.


Assuntos
Métodos Analíticos de Preparação de Amostras/métodos , Braquiúros/química , Hepatopâncreas/química , Lipídeos/isolamento & purificação , Animais , China , Ácidos Graxos/química , Ácidos Graxos/isolamento & purificação , Aromatizantes/química , Aromatizantes/isolamento & purificação , Lipídeos/química
6.
Mar Pollut Bull ; 145: 1-4, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31590765

RESUMO

The total mercury (T-Hg) and methylmercury (MeHg) concentrations in red snow crabs (Chionoecetes japonicus) caught off the coast of Japan were analyzed. The T-Hg concentration ranged from 0.03 to 0.56 mg/kg (mean: 0.21 mg/kg) in the raw muscle, and 0.02 to 0.74 mg/kg (mean: 0.27 mg/kg) in the boiled muscle. The MeHg concentration ranged from 0.04 to 0.54 mg/kg (mean: 0.20 mg/kg) in the raw muscle. The mean ratio of MeHg to T-Hg was 0.88. The crab body weight was found to significantly correlate with the concentrations of T-Hg (r = 0.488) and MeHg (r = 0.490) (p ≤ 0.01). For the general population in Japan, the intake of MeHg from eating red snow crab was estimated to be lower than 0.013 mg/week, which was less than one-sixth of the tolerable MeHg intake (0.08 mg/week).


Assuntos
Braquiúros/química , Mercúrio/análise , Compostos de Metilmercúrio/análise , Frutos do Mar/análise , Animais , Braquiúros/crescimento & desenvolvimento , Braquiúros/metabolismo , Qualidade de Produtos para o Consumidor , Contaminação de Alimentos/análise , Humanos , Japão , Mercúrio/metabolismo , Compostos de Metilmercúrio/metabolismo
7.
Mar Pollut Bull ; 145: 200-207, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31590776

RESUMO

A community-based participatory research was utilized to address the coastal community's concern regarding Deepwater Horizon oil contamination of seafood. Therefore, we analyzed polycyclic aromatic hydrocarbons (PAHs), major toxic constituents of crude oil, in the seafood collected from gulf coast (Louisiana, Alabama and Mississippi) during December 2011-February 2014. PAHs were extracted from edible part of shrimp, oysters, and crabs by the QuEChERS/dsPE procedure and analyzed by gas chromatography-mass spectrometry. The total PAHs data were further analyzed using the General Linear Mixed Model procedure of the SAS (Version 9.3, SAS Institute, Inc., Cary, NC) statistical software. Brown shrimp showed statistically significant differences in PAHs levels with respect to time and locations while white shrimp showed differences at various time points. PAHs levels in oyster and crab samples were not statistically different at the Type I error of 0.05. Overall, the PAHs levels are far below FDA levels of concern for human consumption.


Assuntos
Contaminação de Alimentos/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Alimentos Marinhos/análise , Poluentes Químicos da Água/análise , Alabama , Animais , Braquiúros/química , Monitoramento Ambiental/métodos , Cromatografia Gasosa-Espectrometria de Massas , Louisiana , Mississippi , Ostreidae/química , Penaeidae/química , Poluição por Petróleo/análise
8.
Environ Sci Pollut Res Int ; 26(31): 31581-31591, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31502055

RESUMO

Shellfish cultivation is an expanding economic activity worldwide. However, the rapid development of crab farming and processing result in a large number of crab shells (CS). Utilizing CS could not only benefit the environment and economy but also promote the sustainable development of aquaculture. In this work, it reviews and analyzes recent attempts in CS recycling, including extracting chitin and its derivatives, for use as adsorbent and flocculant and for preparing polymer composites and catalysts, as well as medical applications. The challenges in these utilizations are discussed, and future research directions are proposed as well. Extracting chitin and its derivates, for use as adsorbent and flocculant, are recent major recycling approaches. Preparing polymer composites and carbon materials has gained more and more attentions. Biotechnology is an alternative method for extracting chitin and its derivates from CS, and high-efficiency desalted and deproteinized bacteria need to be screened. Immobilizing the CS-based adsorbents is the key of treating wastewater in continuous systems. Using CS as a biofiller to prepare polymer composites is promising, and surface modification to improve the interfacial compatibility between CS-based fillers and matrix needs to be further studied.


Assuntos
Braquiúros/química , Quitina/análise , Animais , Aquicultura , Bactérias , Materiais Biocompatíveis , Biotecnologia , Quitina/química , Reciclagem
9.
J Photochem Photobiol B ; 199: 111620, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31522113

RESUMO

Current scenario of bio-nanotechnology, successfully fabrication of ultrafine titanium dioxide nanoparticles (TiO2NPs) using various biological protein sources for the multipurpose targets. The present research report involves synthesis of TiO2NPs using antimicrobial peptide (AMP) crustin (Cr). Crustin previously purified from the blue crab, Portunus pelagicus haemolymph, by blue Sepharose CL-6B matrix assisted affinity column chromatography. Synthesized Cr-TiO2NPs was physico-chemically characterized by UV-Visible spectroscopy (UV-Visible), X-ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), High-resolution transmission electron microscopy (HR-TEM) and zeta potential examination. X-ray diffraction analysis for crystalline nature and phase identification of titanium dioxide nanoparticles was absorbed. Functional groups were found through FTIR ranges between 1620 and 1700 cm-1. HR-TEM analysis showed that the synthesized Cr-TiO2NPs tetragonal shape and sizes ranging from 10 to 50 nm. Finally, the surface charge of the Cr-TiO2NPs was confirmed through zeta potential analysis. Furthermore, the characterized Cr-TiO2NPs exhibited good biofilm inhibition against GPB - S. mutans (Gram Positive Bacteria- Streptococcus mutans), GNB - P. vulgaris (Gram Negative Bacteria- Proteus vulgaris) and fungal Candida albicans. Moreover, photocatalysis demonstrated that the Cr-TiO2NPs was effectively explored the degradation of dyes. The results suggest that Cr-TiO2NPs is an excellent bactericidal, fungicidal and photocatalytic agent that can be supportively used for biomedical and industrial applications.


Assuntos
Anti-Infecciosos/química , Peptídeos Catiônicos Antimicrobianos/química , Inseticidas/química , Nanocápsulas/química , Processos Fotoquímicos , Titânio/química , Animais , Anti-Infecciosos/farmacologia , Peptídeos Catiônicos Antimicrobianos/farmacologia , Biofilmes , Braquiúros/química , Candida albicans/efeitos dos fármacos , Catálise , Sobrevivência Celular/efeitos dos fármacos , Culicidae , Liberação Controlada de Fármacos , Humanos , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Luz , Estrutura Molecular , Proteus vulgaris/efeitos dos fármacos , Streptococcus mutans/efeitos dos fármacos
10.
Mar Pollut Bull ; 148: 116-122, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31422296

RESUMO

Mercury is a potent neurotoxin. Its main source of entry into the human body is the consumption of fish and seafood. Within the past few decades a new species of crab has appeared in the food web of the southern Baltic: Rhithropanopeus harrisii. The aim of the present study was to estimate the level of total and labile mercury concentrations in this species collected from the southern Baltic Sea. Analyses were performed using the thermodesorption method. The share of organic mercury fraction, as well as the Hgtot, increased with the size class of the crabs. Due to the increasingly common occurrence of R. harrisii and the low HgTOT concentration in its body, and assuming that it is bound to become a more and more common component of the diet of fish, it can be supposed that the load of toxic mercury entering the food web is likely to decrease.


Assuntos
Braquiúros/química , Mercúrio/química , Animais , Países Bálticos , Braquiúros/metabolismo , Monitoramento Ambiental , Cadeia Alimentar , Contaminação de Alimentos/análise , Humanos , Espécies Introduzidas , Mercúrio/metabolismo , Frutos do Mar/análise , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/metabolismo
11.
J Agric Food Chem ; 67(37): 10458-10469, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31469568

RESUMO

Mud crab (Scylla paramamosain) is a commonly consumed seafood as a result of its high nutritional value; however, it is associated with food allergy. The current understanding of crab allergens remains insufficient. In the present study, an 18 kDa protein was purified from crab muscle and confirmed to be myosin light chain 1 (MLC1) by matrix-assisted laser desorption/ionization-tandem time-of-flight mass spectrometry. Total RNA was isolated and amplified to obtain a MLC1 open reading frame of 462 bp, encoding 154 amino acids. A structural analysis revealed that recombinant MLC1 (rMLC1) expressed in Escherichia coli contained α-helix and random coil. Moreover, rMLC1 displayed strong immunoactivity by dot blot and a basophil activation test. Furthermore, seven allergenic epitopes of MLC1 were predicted, and five critical epitope regions were identified by an inhibition enzyme-linked immunosorbent assay and human mast cell degranulation assay. This comprehensive research of an allergen helps to conduct component-resolved diagnoses and immunotherapies related to crab allergies.


Assuntos
Alérgenos/imunologia , Proteínas de Artrópodes/imunologia , Braquiúros/genética , Clonagem Molecular , Epitopos/imunologia , Cadeias Leves de Miosina/imunologia , Alérgenos/química , Alérgenos/genética , Sequência de Aminoácidos , Animais , Proteínas de Artrópodes/química , Proteínas de Artrópodes/genética , Braquiúros/química , Braquiúros/imunologia , Degranulação Celular , Epitopos/química , Epitopos/genética , Humanos , Mastócitos/imunologia , Cadeias Leves de Miosina/química , Cadeias Leves de Miosina/genética , Fases de Leitura Aberta , Alinhamento de Sequência
12.
J Agric Food Chem ; 67(29): 8253-8267, 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31294564

RESUMO

The study focused on the determination of arsenic species in the top ten most consumed seafoods in the United States. Fifty-four samples were collected from local supermarkets, and their species identities were confirmed by DNA barcoding. The total arsenic in the samples varied greatly in the range of 8-22200 ng/g (wet mass). Speciation analysis based on extraction of water-soluble and nonpolar arsenic showed that inorganic arsenic (iAs) was found only in clams and crabs, while arsenobetaine (AsB) predominates in most samples. Among the other arsenicals, trimethylarsoniopropionate (TMAP) was found in most matrices with higher concentrations in crabs, and arsenosugars existed in most clams and crabs. Nonpolar arsenic accounted for 1-46% of the total arsenic in the samples. The accuracy of the analytical results was evaluated using standard reference materials and spike recovery tests. The survey showed that the iAs concentrations in America's most consumed seafood products are much lower than the tolerable intake set by the Joint FAO/WHO Expert Committee, even at the highest levels found in this study.


Assuntos
Arsênico/química , Contaminação de Alimentos/análise , Alimentos Marinhos/análise , Animais , Bivalves/química , Braquiúros/química , Qualidade de Produtos para o Consumidor , Contaminação de Alimentos/economia , Contaminação de Alimentos/estatística & dados numéricos , Espectrometria de Massas , Alimentos Marinhos/economia , Estados Unidos
13.
Chemosphere ; 230: 559-566, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31125884

RESUMO

Large volume of multidimensional data can be summarised, both in terms of tabulated statistics, and as graphic geospatial visualisations. The latter approach allows rapid interpretation and communication of complex information to stake-holders such as regulators, risk assessors and policy makers. In the main study on polychlorinated naphthalene (PCN), individual samples representing different edible fish species were analysed from around the UK. PCNs were observed in all samples with nearly all of the twelve measured congeners being detected. Summed congener concentrations ranged from 0.7 ng/kg ww (turbot) to 265 ng/kg ww (sprats). The highest contamination levels were recorded for sprats and mackerel with mean summed concentrations of 67 ng/kg ww and 68 ng/kg ww respectively. Two ancillary studies, on potentially toxic elements (PTEs) in crabs from China and aflatoxin in children's blood from Tanzania, demonstrate the wide applicability of this approach. The PTE contents in crab showed strong dependence on the tested tissues and elements, and crabs from Tai and Yangcheng Lakes showed obviously higher PTE levels than the other lakes. Geospatial distribution of the aflatoxin biomarker AF-alb in children's serum from 3 locations showed how individual anthropometric or socio-economic data reveals the relationship between family size, socio-economic score and magnitude of serum aflatoxin levels. In addition to facilitating the flow of interpreted data to stakeholders, these techniques can direct the formulation of risk mitigation activities and help with the identification of data gaps. When combined with hierarchical cluster analyses, correlations within the data can also be predicted.


Assuntos
Aflatoxinas/sangue , Monitoramento Ambiental/métodos , Contaminação de Alimentos/análise , Substâncias Perigosas/análise , Naftalenos/análise , Alimentos Marinhos/análise , Poluentes Químicos da Água/análise , Animais , Biomarcadores/sangue , Braquiúros/química , Criança , China , Sistemas de Informação Geográfica , Substâncias Perigosas/sangue , Humanos , Lagos/análise , Análise Espacial , Tanzânia , Poluentes Químicos da Água/sangue
14.
Mar Drugs ; 17(5)2019 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-31100859

RESUMO

Therapeutic options for Alzheimer's disease, the most common form of dementia, are currently restricted to palliative treatments. The glycosaminoglycan heparin, widely used as a clinical anticoagulant, has previously been shown to inhibit the Alzheimer's disease-relevant ß-secretase 1 (BACE1). Despite this, the deployment of pharmaceutical heparin for the treatment of Alzheimer's disease is largely precluded by its potent anticoagulant activity. Furthermore, ongoing concerns regarding the use of mammalian-sourced heparins, primarily due to prion diseases and religious beliefs hinder the deployment of alternative heparin-based therapeutics. A marine-derived, heparan sulphate-containing glycosaminoglycan extract, isolated from the crab Portunus pelagicus, was identified to inhibit human BACE1 with comparable bioactivity to that of mammalian heparin (IC50 = 1.85 µg mL-1 (R2 = 0.94) and 2.43 µg mL-1 (R2 = 0.93), respectively), while possessing highly attenuated anticoagulant activities. The results from several structural techniques suggest that the interactions between BACE1 and the extract from P. pelagicus are complex and distinct from those of heparin.


Assuntos
Doença de Alzheimer/enzimologia , Secretases da Proteína Precursora do Amiloide/metabolismo , Ácido Aspártico Endopeptidases/metabolismo , Braquiúros/química , Ativação Enzimática/efeitos dos fármacos , Glicosaminoglicanos/farmacologia , Animais , Anticoagulantes/química , Anticoagulantes/isolamento & purificação , Anticoagulantes/farmacologia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Inibidores Enzimáticos/farmacologia , Glicosaminoglicanos/química , Glicosaminoglicanos/isolamento & purificação
15.
J Trace Elem Med Biol ; 54: 8-20, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31109625

RESUMO

Recently, biogenic nanoparticles have been considered promising candidates for manufacturing antibacterial nanodrugs. Here, we synthesized AgNPs using the crab-borne antibacterial agent hemocyanin and assessed the antibacterial action against several pathogenic bacteria. In this study, the crustacean immune protein hemocyanin (Pp-Hc, 78 kDa) purified from Portunus pelagicus hemolymph was used to fabricate silver nanoparticles. Characterization of hemocyanin-fabricated AgNPs (Pp-Hc AgNPs) were achieved using ultraviolet-visible spectrophotometer, X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), high-resolution-transmission electron microscopy (HR-TEM), and energy-dispersive X-ray spectroscopy. The antibacterial efficacy of AgNO3,Pp-Hc and Pp-Hc AgNPs was compared by growth inhibition, antibiofilm and live and dead analyses. Based on the results, Pp-Hc AgNPs was more efficient than Pp-Hc and AgNO3 against pathogenic bacteria. Mechanistic analysis revealed membrane damage and reactive oxygen species (ROS) generation, suggesting that Pp-Hc and Pp-Hc AgNPs rely to similar modes of action. Intracellular protein molecules and nucleic acid leakage confirmed that Pp-Hc AgNPs increase membrane permeability, leading to cell death. Based on our results, capping of the exterior surface of nanoparticles with antimicrobial crab-borne peptides, such as Pp-Hc, improves their functions as potential agents against bacterial diseases, which may be useful in clinical applications.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Braquiúros/química , Hemocianinas/química , Hemocianinas/farmacologia , Nanopartículas Metálicas/química , Prata/química , Animais , Bactérias/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Fragmentação do DNA/efeitos dos fármacos , Malondialdeído/metabolismo , Testes de Sensibilidade Microbiana , Peroxidase/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Superóxido Dismutase/metabolismo
16.
Biomed Pharmacother ; 114: 108841, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30981106

RESUMO

Porcupine bezoar (PB) is a calcified undigested material generally found in porcupine's (Hystrix brachyura) gastrointestinal tract. The bezoar is traditionally used in South East Asia and Europe for the treatment of cancer, poisoning, dengue, typhoid, etc. However, limited scientific studies have been performed to verify its anticancer potential to substantiate its traditional claims in the treatment of cancers. Hence, this study was aimed at investigating the in vitro and in vivo anticancer properties of two grassy PB aqueous extract (PB-A and PB-B) using A375 cancer cell line and zebrafish model, respectively. This paper presents the first report on in vitro A375 cell viability assay, apoptosis assay, cell cycle arrest assay, migration assay, invasion assay, qPCR experimental assay and in vivo anti-angiogenesis assay using the grassy PBs. Experimental findings revealed IC50 value are 26.59 ± 1.37 µg/mL and 30.12 ± 3.25 µg/mL for PB-A and PB-B respectively. PBs showed anti-proliferative activity with no significant cytotoxic effect on normal human dermal fibroblast (NHDF). PBs were also found to induce apoptosis via intrinsic pathway and arrest cell cycle at G2/M phase. Additionally, the findings indicated its ability to debilitate migration and invasion of A375 cells. Further evaluation using embryo zebrafish model revealed LC50 = 450.0 ± 2.50 µg/mL and 58.7 ± 5.0 µg/mL for PB-A and PB-B which also exerted anti-angiogenesis effect in zebrafish. Moreover, stearic acid, ursodeoxycholic acid and pregnenolone were identified as possible metabolites that might contribute to the anticancer effect of the both PBs. Overall, this study demonstrated that PB-A and PB-B possess potential in vitro and in vivo anticancer effects which are elicited through selective cytotoxic effect, induction of apoptosis, inhibition of migration and invasion and anti-angiogenesis. This study provides scientific evidence that the porcupine bezoar do possess anti-cancer efficacy and further justifies its traditional utility. However, more experiments with higher vertebrae models are still warranted to validate its traditional claims as an anticancer agent.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Bezoares/metabolismo , Braquiúros/química , Neoplasias/tratamento farmacológico , Porcos-Espinhos/metabolismo , Animais , Apoptose/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Linhagem Celular , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Modelos Animais de Doenças , Fibroblastos/efeitos dos fármacos , Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos , Humanos , Peixe-Zebra
17.
Food Chem ; 289: 413-418, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-30955631

RESUMO

In Taiwan, crab is one of the main causes for food allergy. Several proteins are recognized as crustacean allergens, and tropomyosin is known to be the major one. However, sensitization patterns of Taiwanese patients to crustacean allergens remain unclear. Therefore, we analyzed the specific-IgE binding ability of crucifix crab (Charybdis feriatus) allergens by western blot using patients' sera. In particular, we found a 56 kDa protein in crucifix crab reacted with specific-IgEs in patients' sera, and we further identified the protein as a novel crab allergen pyruvate kinase 2. Additionally, little is known about tropomyosin contents in crabs consumed in Taiwan. Thus, we also quantified the levels of tropomyosin by using enzyme-linked immunosorbent assay (ELISA) among raw and cooked crab species. Our results showed tropomyosin levels varied depending on crab species. In summary, these findings improve the understanding of crustacean allergens and contribute to the clinical diagnosis of crustacean allergies.


Assuntos
Alérgenos/análise , Braquiúros/imunologia , Piruvato Quinase/análise , Piruvato Quinase/imunologia , Frutos do Mar/análise , Adulto , Animais , Western Blotting , Braquiúros/química , Ensaio de Imunoadsorção Enzimática , Hipersensibilidade Alimentar/sangue , Hipersensibilidade Alimentar/imunologia , Humanos , Imunoglobulina E/sangue , Alimentos Marinhos/análise , Taiwan , Tropomiosina/análise
18.
Mar Pollut Bull ; 141: 273-282, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30955735

RESUMO

The concentrations of nine heavy metals accumulated in different organs of two crab species collected from the South East coast of India were estimated. The order of trace metal concentration in different organs of Portunus pelagicus was Cu > Mn > Cd > Ni > Pb > Co > Hg = Cr = U in gills, Cu > Mn > Cd > Ni > Pb = Co > Hg > Cr = U in hepatopancreas, and Cu > Cr > Ni > Mn > Cd > Pb > Co > Hg > U in muscles, whereas that for Portunus sanguinolentus was Cu > Mn > Cd > Ni > Pb > Co > Hg > Cr = U in gills, Cu > Mn > Cd > Ni > Pb = Co > U > Cr = Hg in hepatopancreas, and Cr > Cu > Ni > Mn > Cd > Co > Pb > Hg = U in muscles. The order of trace metal uptake for different organs was gills > hepatopancreas > muscles. Individual mean bioaccumulation index (IMBI) values varied between 0.0 and 0.52, 0.0 and 0.28, and 0.06 and 0.30 for gills, hepatopancreas, and muscles, respectively. Cr in muscles of P. sanguinolentus and Cu and Cd in all organs of both the species were found to be higher than the maximum permitted concentration recommended by food safety guidelines. Target hazard quotient (THQ) results suggested that there is a potential risk due to Co, Cd, and Cr, particularly for children, if the crab consumption frequency is more than once a month.


Assuntos
Braquiúros/química , Monitoramento Ambiental/métodos , Metais Pesados/análise , Alimentos Marinhos/análise , Oligoelementos/análise , Animais , Criança , Brânquias/química , Hepatopâncreas/química , Humanos , Índia , Medição de Risco , Poluentes Químicos da Água/análise
19.
Chemosphere ; 226: 340-350, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30939373

RESUMO

We analyzed the distribution and bioaccumulation of six heavy metals (Cu, Pb, Zn, Cr, Cd and Hg) in marine organisms, water, and sediments from China's Hainan and Zhoushan coastal regions. In all marine organism, seawater, and sediment samples, Zn and Hg had the highest and lowest concentrations, respectively. Heavy metals in marine organisms varied by region and species, with concentrations being higher in Zhoushan than in Hainan (except for Zn) and in crab than in fish. Marine organisms' ability to digest and eliminate heavy metals (bioaccumulation ability), based on bioaccumulation factors, was significantly higher for heavy metals in seawater than in sediment. Higher sediment background values may explain the higher heavy metal concentrations in crab. Generally, marine organisms' bioaccumulation ability was higher for Cu and Zn and lower for Pb. Findings specific to the coastal environments may be generalizable to other coastal areas in China or worldwide.


Assuntos
Organismos Aquáticos/metabolismo , Monitoramento Ambiental , Metais Pesados/análise , Poluentes Químicos da Água/análise , Animais , Braquiúros/química , Braquiúros/metabolismo , China , Peixes/metabolismo , Sedimentos Geológicos/química , Água do Mar/química
20.
Mar Drugs ; 17(4)2019 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-31013628

RESUMO

Chitin was extracted from Polybius henslowii, a swimming crab, captured in large quantities throughout the Portuguese coast by purse seine vessels as bycatch. After standard chitin extraction procedures, water-soluble chitosan products were obtained via two different methods: (1) N-acetylation with the addition of acetic anhydride and (2) a reaction with hydrogen peroxide. The chemical structure and molecular weight of chitosan derivatives, water-soluble chitosan (WSC) and chitooligosaccharides (COS), were confirmed by Fourier Transform Infrared Spectroscopy (FT-IR) and gel permeation chromatography (GPC). Antioxidant and metal chelation activities were evaluated, and the growth inhibition capacity was tested on four phytopatogens. The chitooligosaccharides from pereopods (pCOS) and shell body parts (sCOS) inhibited all fungal species tested, particularly Cryphonectria parasitica with 84.7% and 85.5%, respectively. Both radical scavenging and antifungal activities proved to be dose-dependent. Chitooligosaccharides with a low molecular weight (2.7, 7.4, and 10.4 Kg·mol-1) showed the highest activity among all properties tested. These results suggested that chitosan derivatives from P. henslowii raw material could potentially be used against phytopathogens or as ingredient in cosmetics and other products related to oxidative stress.


Assuntos
Antifúngicos/farmacologia , Antioxidantes/farmacologia , Braquiúros/química , Quitosana/farmacologia , Animais , Antifúngicos/química , Antioxidantes/química , Quitina/análogos & derivados , Quitina/química , Quitina/farmacologia , Quitosana/química , Fungos/efeitos dos fármacos , Peróxido de Hidrogênio/farmacologia , Peso Molecular , Polímeros/química , Polímeros/farmacologia , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Água
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