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1.
Food Chem ; 302: 125347, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31430631

RESUMO

This work aims to prolong the storage stability of polyphenols, obtained from grape pomace, using a spray drying-based microencapsulation technique. The microcapsules obtained under optimal conditions were stored at two different relative humidities (33% and 52%) during 75 days. The analyses of total phenolic content, antioxidant activity, and individual phenolic compounds were carried out every 15 days, and the most stable microcapsules were achieved with maltodextrin DE4-7 prepared by adding gum Arabic to the wall material at a ratio of 8:2. The phenolic content loss rate was found to be in a range of 0.93-5.42 % depending on phenolic compound. The decrease in the content of rutin, chlorogenic acid, epicatechin, caffeic acid, gallic acid, caftaric acid and catechin was only 0.93, 2.09, 2.13, 2.27, 2.41, 3.40 and 5.42%, respectively. These results indicate more efficient storage conditions than those of previously reported studies.


Assuntos
Cápsulas/química , Armazenamento de Alimentos , Polifenóis/química , Vitis/química , Antioxidantes/análise , Antioxidantes/química , Catequina/análise , Catequina/química , Ácido Clorogênico/análise , Ácido Clorogênico/química , Composição de Medicamentos/métodos , Goma Arábica/química , Umidade , Fenóis/análise , Fenóis/química , Extratos Vegetais/química , Polifenóis/análise , Polissacarídeos/química , Rutina/análise , Rutina/química
2.
Zhongguo Zhong Yao Za Zhi ; 44(14): 2953-2959, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31602839

RESUMO

This study aimed to investigate the protective effect and preliminary mechanism of Danzhi Jiangtang Capsules( DJC) on liver of hyperlipidemic rats. The hyperlipidemia models were successfully made by high-fat diet for 12 weeks in male SD rats,and then divided into model control group and DJC treatment groups( 500 and 1 000 mg·kg~(-1)·d-1) via gavage administration for additional 8 weeks.The levels of serum lipid and liver metabolism indices were detected; HE and oil red O staining were used to observe the pathological changes of liver. Expression levels of extracellular regulated protein kinase 1/2( ERK1/2),c-Jun N-terminal kinase( JNK),and p38 mitogen-activated protein kinase( p38 MAPK) were detected by real-time polymerase chain reaction( RT-PCR). Expression of MCP-1,phosphorylated ERK( p-ERK),phosphorylated JNK( p-JNK),and phosphorylated p38 MAPK( p-p38) were analyzed by immunohistochemistry and Western blot. The results showed that DJC decreased body weight and serum levels of total cholesterol( TC),triglyceride( TG),alanine aminotransferase( ALT),aspartate aminotransferase( AST),increased serum high-density lipoprotein cholesterol( HDL-C) level,ameliorate injury and lipid deposition in the liver induced by the high-fat diet,decreased mRNA expression of ERK1/2,JNK and p-38 MAPK as well as protein expression of p-ERK,p-JNK,p-p38,and MCP-1,somewhat showing a dose-dependent effect. Therefore,DJC has an obvious protective effect on liver of hyperlipidemic rats with certain dose-dependent effect,and the mechanism may be related with inhibiting MAPK pathways and inflammation.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Hiperlipidemias/tratamento farmacológico , Sistema de Sinalização das MAP Quinases , Animais , Cápsulas , Dieta Hiperlipídica , Inflamação , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Ratos , Ratos Sprague-Dawley , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
3.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3816-3824, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602958

RESUMO

To systemically evaluate the benefits and side effects of Shensong Yangxin Capsules( SYC) in the adjuvant treatment of stable angina pectoris( SAP). Chinese and English databases( PubMed,EMbase,the Cochrane Library,CBM,CNKI,VIP,Wan Fang database) were retrieved to collect the randomized controlled trials( RCTs) about therapeutic efficacy of SYC combined with routine drug( trial group) vs routine drug( control group) in the treatment of SAP. The methodological quality of the RCTs was evaluated based on the cochrane risk of bias assessment tool. The data were extracted and Meta-analyzed by Reviewer Manager 5. 3. TSA 0. 9 software was used for trial sequential analysis( TSA) of the total effective rate of symptoms improvement. A total of 15 RCTs with 1 316 participants were included. RESULTS:: of Meta-analysis showed that the total effective rate of angina symptoms improvement( RR = 1. 15,95% CI[1. 09,1. 21],P<0. 001) of trial group were significantly higher than those of control group,with statistical significance,the total effective rate of electrocardiograms( ECG) improvement( RR = 1. 10,95% CI[0. 94,1. 29],P = 0. 25) of trial group were significantly higher than those of control group,but the difference was not statistically significant. After treatment,the improvement of the total time of 24 h general ischemia( SMD =-1. 21,95%CI[-1. 97,-0. 45],P = 0. 002),the ST-segment depression amplitude( SMD =-1. 30,95%CI [-1. 52,-1. 09],P<0. 001),the duration of angina pectoris attack( SMD =-1. 16,95% CI[-1. 36,-0. 95],P< 0. 001),the angina pectoris attack every week( SMD =-0. 80,95%CI[-1. 10,-0. 50],P<0. 001),the onsumption of nitroglycerin every week( SMD=-0. 72,95%CI[-1. 05,-0. 39],P<0. 001) in the trial group were better than that of the control group,and the difference was statistically significant. Besides,the improvement of the blood lipid and high sensitivity C reactive protein( hs-CRP) in the trial group were better than those of the control group after treatment,and the difference was statistically significant( P< 0. 001). Funnel plots and Egger's linear regression showed that there was no publication bias. By sensitivity analysis,it showed that the results of this study were stable and reliable. No obvious adverse drug reactions were observed in all studies. TSA analysis showed that the evidence of Meta-analysis was reliable. SYC combined with routine Western medicine treatment for SAP can improve the total effective rate of angina pectoris,reduce 24 h total ischemia time,ST segment depression amplitude,duration of angina pectoris attack,frequency of angina pectoris attack and nitroglycerin dosage,and also can improve blood lipid and hs-CRP levels.


Assuntos
Angina Estável/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Adjuvantes Farmacêuticos , Proteína C-Reativa/análise , Cápsulas , Eletrocardiografia , Humanos , Lipídeos/sangue , Ensaios Clínicos Controlados Aleatórios como Assunto
4.
Eur J Pharm Biopharm ; 144: 174-179, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31541663

RESUMO

Capsules are a widely used oral dosage form due to their simplicity and ease of manufacture. They are equally popular for both pharmaceutical and nutraceutical products and since they do not need extensive formulation development, it is a dosage form of choice for new drugs undergoing animal or clinical trials. In addition to the standard hard-gelatin or cellulose-based vegetarian capsules, functional capsules such as those with built-in gastroresistance would be of great value. In this work, commonly used enteric polymers were investigated for the production of hard-capsules. The polymers used in this study included cellulose derivatives (HPMC AS-LF and HP-55) and acrylic/methacrylic acid derivatives (EUDRAGIT L100 and S100). A range of concentrations of polymers and plasticisers were tested to optimise the formulation for the production of capsule shells with desirable physicochemical and gastroresistance characteristics. Drug release from optimised capsules produced from HPMC AS-LF, HP-55, EUDRAGIT L100 and S100 was shown to be comparable to drug release from corresponding polymer-coated tablets in both compendial and physiological bicarbonate buffer. In summary, herein we report a simple method for producing enteric capsule shells which do not need an additional coating step which, if validated at large scale, can significantly reduce the cost of manufacturing of conventional enteric coated dosage forms. These capsules are also likely to improve the inter-tablet variability in post-gastric drug release inherent in conventional dosage forms due to coating variability.


Assuntos
Cápsulas/química , Polímeros/química , Bicarbonatos/química , Tampões (Química) , Celulose/química , Química Farmacêutica/métodos , Preparações de Ação Retardada/química , Sistemas de Liberação de Medicamentos/métodos , Liberação Controlada de Fármacos/efeitos dos fármacos , Excipientes/química , Gelatina/química , Concentração de Íons de Hidrogênio , Metacrilatos/química , Metilcelulose/análogos & derivados , Metilcelulose/química , Ácidos Polimetacrílicos/química , Solubilidade/efeitos dos fármacos , Comprimidos/química
6.
Braz Oral Res ; 33(0): e089, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31531552

RESUMO

The objectives of this double-blind randomized clinical trial were to compare (a) the clinical times and (b) the occurrence and severity of postoperative sensitivity, of posterior restoration that used a universal adhesive, in a self-etch or selective enamel-etching technique, along with incremental or bulk-fill composites (presented in syringes or capsules). A total of 295 posterior restorations were placed according to the following groups: SETB - self-etch/bulk-fill in syringe; SETC - self-etch/bulk-fill in capsules; SETI - self-etch/incremental; SEEB - selective enamel-etching/bulk-fill in syringe; SEEC - selective enamel-etching/bulk-fill in capsules; and SEEI - selective enamel-etching/incremental. Clinical time was assessed by a reason (s/mm3) between the total volume of resin inserted and the total time required to perform the restorations. Postoperative sensitivity was evaluated using two scales (Numeric Rating Scale and Visual Analogue Scale). Mean clinical time results, analyzed by Wald's Chi-square, showed significant statistical differences among all groups (p<0.001), indicating that the restorative strategy affected the time required for the restoration. A generalized estimating equation model statistical analysis, performed to compare postoperative sensitivity, showed that neither the restorative technique, the adhesive strategy nor the presentation mode of the bulk-fill composite affected the overall risk of postoperative sensitivity (4.06 [2.22-6.81]). The use of bulk-fill composite, presented in capsules or syringes, is less time consuming and does not increase the risk or intensity of postoperative sensitivity relative to the traditional incremental technique.


Assuntos
Resinas Compostas/uso terapêutico , Restauração Dentária Permanente/efeitos adversos , Sensibilidade da Dentina/etiologia , Cápsulas , Método Duplo-Cego , Feminino , Humanos , Masculino , Complicações Pós-Operatórias/etiologia , Valores de Referência , Reprodutibilidade dos Testes , Fatores de Risco , Seringas , Fatores de Tempo , Resultado do Tratamento
7.
Exp Parasitol ; 205: 107735, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31381870

RESUMO

Two experiments were performed to determine whether oral administration of copper oxide capsules controlled helminthic infections in Lacaune sheep without acute collateral effects on animal health. In experiment 1, 48 multiparous lactating sheep (60.1 ±â€¯8.5 kg) were stratified according to initial number of eggs (Haemonchus contortus) per gram of feces (EPG) and were assigned randomly to 1 of two treatments (24 sheep/treatment): no oral administration (control) or oral administration of two copper capsules (treated; approximately 58 mg copper/kg body weight). Blood and fecal samples were collected on days 0, 15 and 45. Animals treated with copper capsules showed lower of EPG, eosinophils, acetylcholinesterase (AChE) in whole blood, and lower butyrylcholinesterase (BChE) activity in serum. Treated sheep had higher erythrocyte numbers, hemoglobin concentrations, hematocrit, and lymphocyte numbers. In experiment 2, 12 male lambs negative for helminths and coccidia were assigned randomly to one of two treatments (six lambs/treatment): control or treated (one copper capsule; approximately 58 mg copper/kg body weight); the experiment was designed to determine whether the results of experiment 1 were due to treatment or parasitism. Blood samples were collected on days 0, 5, 10 and 15 and fecal samples were collected on days 0, 7 and 15. Treated animals showed greater concentrations of lymphocytes; however, treatment had no effect on other hemogram variables, AChE and BChE activities, or levels of alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, creatinine, urea, albumin, total protein, and reactive oxygen species. These data suggest that copper capsules in dairy sheep efficiently controlled H. contortus infections. Treatment was not harmful to lambs during the first 15 days, i.e. there were no signs of acute toxicity.


Assuntos
Cobre/administração & dosagem , Hemoncose/veterinária , Helmintíase Animal/tratamento farmacológico , Lactação , Doenças dos Ovinos/tratamento farmacológico , Acetilcolinesterase/sangue , Administração Oral , Animais , Butirilcolinesterase/sangue , Cápsulas , Cobre/uso terapêutico , Indústria de Laticínios , Resíduos de Drogas , Eosinófilos/efeitos dos fármacos , Contagem de Eritrócitos/veterinária , Fezes/parasitologia , Feminino , Hemoncose/tratamento farmacológico , Hemoncose/prevenção & controle , Helmintíase Animal/sangue , Helmintíase Animal/prevenção & controle , Hematócrito/veterinária , Hemoglobinas/análise , Contagem de Linfócitos/veterinária , Masculino , Leite/química , Contagem de Ovos de Parasitas/veterinária , Paridade , Distribuição Aleatória , Ovinos , Doenças dos Ovinos/sangue , Doenças dos Ovinos/parasitologia , Doenças dos Ovinos/prevenção & controle
8.
Int J Nanomedicine ; 14: 6001-6018, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31447554

RESUMO

Background: The gastroretentive drug delivery system is an effective administration route, which can improve the bioavailability of the drug and the therapeutic effect by prolonging the release time of the drug and controlling the release rate in the stomach. Methods: Inspired by the excellent adhesion properties of mussel protein, we prepared novel catechol-grafted chitosan alginate/barium sulfate microcapsules (Cat-CA/BS MCs) with mucoadhesive properties and computed tomography (CT) imaging function for gastric drug delivery. First, barium sulfate nanoclusters used as CT contrast agent were synthesized in situ in the Cat-CA/BS MCs through a one-step electronic spinning method. Next, catechol-grafted chitosan as the mucoadhesive moiety was coated on the surface of Cat-CA/BS MCs by polyelectrolyte molecule self-assembly. Results: The prepared Cat-CA/BS MCs could effectively retained in the stomach for 48 hours and successively released ranitidine hydrochloride, which could be used for the treatment of gastric ulcer. Cat-CA/BS MCs exhibited superior CT contrast imaging properties for real-time tracking in vivo after oral administration. Conclusion: These findings demonstrate that Cat-CA/BS MCs serving as multifunctional oral drug carriers possess huge potential in gastroretentive drug delivery and non-invasive visualization.


Assuntos
Sulfato de Bário/química , Cápsulas/química , Catecóis/farmacologia , Quitosana/química , Sistemas de Liberação de Medicamentos , Estômago/efeitos dos fármacos , Estômago/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Adesividade , Administração Oral , Animais , Preparações de Ação Retardada/farmacologia , Portadores de Fármacos , Feminino , Camundongos Endogâmicos C57BL , Muco/química , Nanopartículas/química , Nanopartículas/ultraestrutura , Ranitidina/farmacologia , Espectroscopia de Luz Próxima ao Infravermelho , Estômago/patologia
9.
Food Chem ; 300: 125171, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31330369

RESUMO

This study aimed to examine the modification effect of whey protein concentrate (WPC), WPC-gum arabic (WPC-GA) or WPC-high methoxyl pectin (WPC-PEC) complex to tailor-modify W/O/W emulsion for secondary microencapsulation of hydrophilic arbutin and hydrophobic coumaric acid. The stability and rheological properties of coated emulsions, encapsulation yield, release and degradation kinetics of arbutin and coumaric acid were investigated. Results revealed that WPC-PEC complex (at the ratio of 1:3) coating W/O/W emulsion exhibited the highest viscosity and stability, with the highest encapsulation yield of 91.08% for arbutin and 80.92% for coumaric acid, respectively. Tighter coating structure of the WPC-PEC complex (1:3) forming a stronger gel network structure was confirmed, accounting for the larger mean particle size of 569.67 nm. Moreover, the WPC-PEC (1:3) coating W/O/W emulsion also showed controlled release of arbutin and coumaric acid in simulated conditions. The k value of degradation kinetics for arbutin (7.99 × 10-4 at pH = 1.2, 4.19 × 10-4 at 90 °C and 7.52 × 10-4 at UV-C treatment) and coumaric acid (5.18 × 10-4 at pH = 1.2, 3.24 × 10-4 at 90 °C and 6.90 × 10-4 at UV-C treatment) indicated low degradation rate. The present study revealed that the WPC-PEC (1:3) coating W/O/W emulsion could provide a better synergistic effect on higher encapsulation yield and stability of arbutin and coumaric acid.


Assuntos
Arbutina/química , Cápsulas/química , Ácidos Cumáricos/química , Emulsões/química , Polissacarídeos/química , Arbutina/farmacocinética , Composição de Medicamentos , Goma Arábica/química , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Tamanho da Partícula , Pectinas/química , Reologia , Temperatura Ambiente , Raios Ultravioleta , Viscosidade , Proteínas do Soro do Leite/química
10.
Food Chem ; 300: 125174, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31330370

RESUMO

This paper focuses on the use of a new type of yeast encapsulation procedure, applying the chitosan-calcium alginate double layer microcapsules, for the production of Riesling sparkling wine. Four different sparkling wines were produced by free or encapsulated yeasts. The four types of yeast used were adapted (Free EtOH-A, Encapsulated EtOH-A) and non-adapted to ethanol (Free, Encapsulated). The different yeast-inoculating formats had a significant impact on oxygen consumption and pressure increase rate in the bottle during the prise de mousse. Similarly to the free form, encapsulated yeast successfully completed the secondary fermentation. After an ageing period of 6 months, volatiles and sensory profiles of sparkling wines were compared. Although, some differences in volatile profiles were found among samples, sparkling wines produced by Encapsulated EtOH-A showed sensory properties, in terms of aroma, taste and body, similar to those produced by free yeast (both adapted and non-adapted to ethanol).


Assuntos
Alginatos/química , Quitosana/química , Saccharomyces cerevisiae/fisiologia , Vinho/microbiologia , Adulto , Idoso , Cápsulas , Etanol/química , Etanol/farmacologia , Feminino , Fermentação , Humanos , Masculino , Pessoa de Meia-Idade , Odorantes , Pressão , Saccharomyces cerevisiae/efeitos dos fármacos , Paladar , Compostos Orgânicos Voláteis/análise , Vinho/análise
11.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2637-2643, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359734

RESUMO

To investigate the effect of Fuyanshu Capsules combined with Western medicine antibiotics on symptoms and inflammatory factors IL-10 and IL-1ß in patients with pelvic inflammatory disease and its possible mechanism. Totally 112 patients with pelvic inflammatory disease of damp-heat stagnation treated since April 2017 to April 2018 were randomly divided into treatment group( group A,57 cases) and control group( group B,55 cases). The treatment group was given Fuyanshu Capsules for 56 d,and levofloxacin hydrochloride tablets and metronidazole tablets for 14 d. The control group was given Fuyanshu Capsules as its analogue. The curative rate,effective rate and inefficiency,serum IL-10 and IL-1ß levels were compared between the two groups. The curative effect was evaluated with McCormack score and traditional Chinese medicine( TCM) syndrome score. The recurrence rate and chronic pelvic pain were followed up after one menstrual cycle. It was found that the curative rate and effective rate of group A were higher than those of group B after treatment. After 28 d of treatment,there was a difference in the effective rate of TCM syndrome score between group A and group B( 62. 71% vs 8. 47%,P < 0. 01). After 56 d of treatment,serum IL-10 increased,while IL-1ß decreased in group A,which was significantly different from that in group B( P<0. 01). The recurrence rate of PID and chronic pelvic pain in group A were significantly lower than those in group B( P<0. 01). The results showed that Fuyanshu Capsules combined with levofloxacin and metronidazole could alleviate the clinical symptoms and signs of chronic pelvic inflammation of damp-heat stagnation type,reduce the recurrence rate of pelvic inflammation,relieve pelvic pain,and alleviate the inflammation status of patients by regulating the expression of IL-10 and IL-1ß in peripheral serum.


Assuntos
Antibacterianos/uso terapêutico , Medicamentos de Ervas Chinesas/uso terapêutico , Doença Inflamatória Pélvica/tratamento farmacológico , Cápsulas , Feminino , Humanos , Interleucina-10/sangue , Interleucina-1beta/sangue , Levofloxacino , Medicina Tradicional Chinesa , Metronidazol
12.
Food Chem ; 298: 125045, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31261002

RESUMO

In this study, sacha inchi oil (SIO) (Plukenetia volubilis L.) was microencapsulated via complex coacervation of ovalbumin (OVA) and sodium alginate (AL), and the microcapsule properties were characterized. The omega-3 content in the SIO was evaluated after in vitro gastric simulation and microencapsulation. The coacervate complex between OVA and AL was evaluated based on electrostatic interactions and developed for use as a wall material via the SIO microencapsulation process. The best mass ratio for the biopolymers (OVA:AL) was 4:1 at pH 3.8, and the complex exhibited a thermal resistance at 189.86 °C. The SIO microcapsules showed a high encapsulation efficiency of approximately 94.12% in the ratio (OVA:AL) of 1:1. Furthermore, microencapsulated SIO presented resistance under gastric conditions with a low release of acyl (ω-3) units. These results demonstrate that it is possible to use OVA:AL as encapsulating agents to protect bioactive compounds and to improve the thermal behavior of microcapsules.


Assuntos
Composição de Medicamentos/métodos , Euphorbiaceae/metabolismo , Óleos Vegetais/química , Alginatos/química , Varredura Diferencial de Calorimetria , Cápsulas/química , Euphorbiaceae/química , Ácidos Graxos Ômega-3/química , Concentração de Íons de Hidrogênio , Ovalbumina/química , Eletricidade Estática
13.
Zhongguo Zhong Yao Za Zhi ; 44(11): 2317-2323, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359658

RESUMO

This paper was mainly to discuss the potential role and mechanism of Lianhua Qingwen Capsules(LHQW) in inhibiting pathological inflammation in the model of acute lung injury caused by bacterial infection. For in vitro study, the mRNA expression of MCP-1 in RAW264.7 cells and THP-1 cells, the content of MCP-1 in cell supernatant, as well as the effect of LHQW on chemotaxis of macrophages were detected. For in vivo study, mice were randomly divided into 7 groups, including normal group, model group(LPS 5 mg·kg~(-1)), LHQW 300, 600 and 1 200 mg·kg~(-1)(low, middle and high dose) groups, dexamethasone 5 mg·kg~(-1) group and penicillin-streptomycin group. Then, the anal temperature was detected two hours later. Dry weight and wet weight of lung tissues in mice were determined; TNF-α and MCP-1 levels in alveolar lavage fluid and MCP-1 in serum were detected. In addition, the infiltration of alveolar macrophages was also observed and the infiltration count of alveolar macrophages was measured by CCK-8 method. HE staining was also used to observe the inflammatory infiltration of lung tissues in mice. Both of the in vitro and in vivo data consistently have confirmed that: by down-regulating the expression of MCP-1, LHWQ could efficiently decrease the chemotaxis of monocytes toward the pulmonary infection foci, thus blocking the disease development in ALI animal model.


Assuntos
Lesão Pulmonar Aguda/microbiologia , Infecções Bacterianas/tratamento farmacológico , Quimiotaxia , Medicamentos de Ervas Chinesas/farmacologia , Macrófagos/efeitos dos fármacos , Animais , Líquido da Lavagem Broncoalveolar , Cápsulas , Quimiocina CCL2/metabolismo , Humanos , Lipopolissacarídeos , Pulmão , Camundongos , Células RAW 264.7 , Distribuição Aleatória , Células THP-1 , Fator de Necrose Tumoral alfa/metabolismo
14.
Zhongguo Zhong Yao Za Zhi ; 44(11): 2324-2330, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359659

RESUMO

The aim of this paper was to investigate the preventive effects of Keluoxin Capsules(KLX) on diabetic retinopathy in db/db mice. One hundred male db/db diabetic mice(45-55 g, 8 weeks) were randomly divided into 5 groups(model, KLX low dose, KLX middle dose, KLX high dose, Dobesilate) and 20 male C57 BL/KsJdb~(+/+) were taken as control group. Body weight and fasting blood-glucose were detected every week. Mice were administrated with saline(control and model group), KLX(780, 1 560, 3 120 mg·kg~(-1)·d~(-1), ig), Dobesilate(195 mg·kg~(-1)·d~(-1), ig) for 20 weeks, respectively. At the end of the administration, optical coherence tomography, fundus fluorescein angiography and electroretinogram of the retina were measured. The eyeball was extirpated and retina was isolated to make paraffin section, followed by HE staining and glial fibrillary acidic protein(GFAP) immunohistochemistry. The results indicated that KLX has no obvious effect on body weight and fasting blood level in db/db mice. However, KLX could significantly regulate the thickness of retinal ganglion layer and inner plexiform layer. KLX was able to remarkably reduce the quantity of diabetic microvessel. Meanwhile, KLX could notably improve retinal function. Moreover, KLX could observably modulate the cell arrangement and edema in each layer. There was no markable difference in retina according to the immunochemistry assay. In the present study, KLX exert marked preventive effects on diabetic retinopathy in db/db mice, which provided an experimental evidence for clinical use.


Assuntos
Diabetes Mellitus Experimental , Retinopatia Diabética/tratamento farmacológico , Hipoglicemiantes/farmacologia , Animais , Cápsulas , Angiofluoresceinografia , Masculino , Camundongos , Distribuição Aleatória , Retina/efeitos dos fármacos
15.
Food Chem ; 298: 125079, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260959

RESUMO

Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%). The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin. The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ±â€¯0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ±â€¯0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration. Furthermore, our results showed that the antibacterial activity of the obtained microcapsules significantly depends on both citral concentration and interfacial membrane composition. The presence of two layers surrounding the citral droplets may result in a progressive and controlled release of the encapsulated citral.


Assuntos
Emulsões/química , Monoterpenos/química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Cápsulas/química , Caseínas/química , Dessecação , Listeria/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Tamanho da Partícula , Pectinas/química , Polissacarídeos/química , Staphylococcus aureus/efeitos dos fármacos
16.
Zhongguo Zhong Yao Za Zhi ; 44(13): 2858-2864, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31359701

RESUMO

To evaluate the efficacy and safety of Gantaishu Capsules in the treatment of viral B hepatitis. The randomized controlled trials( RCT) retrieved from Cochrane Library,PubMed,Sino Med,CNKI,Wan Fang and VIP were enrolled. The methodology quality of the included studies was evaluated,and a Meta-analysis was performed using Rev Man 5. 3 software. A total of six randomized controlled trials were included. Meta-analysis results showed that the similarities in the negative conversion rate of HBe Ag( RR = 2. 09,95%CI[0. 90,4. 85],P = 0. 09,I2= 0%),the HBV-DNA negative rate( RR = 1. 49,95% CI[0. 56,3. 95],P = 0. 43,I2= 0%) and the changes in ALT levels before and after treatment( RR =-6. 28,95%CI[-72. 83,60. 27],P = 0. 85,I2= 99%),with no statistical difference. In terms of quality of life,Gantaishu Capsules can significantly alleviate the symptoms of hepatitis B patients,with less adverse reactions. Gantaishu Capsules and Dongbao Gantai Tablets were similar in antiviral effect. In this term,Gantaishu Capsules was superior to Dangfei Liganning Capsules. It can significantly alleviate the symptoms of chronic hepatitis B patients,with a good clinical safety.Therefore,it can be applied in the case of syndrome differentiation and treatment. In view of the low quality of the included studies,more high-quality clinical trials were required to confirm its efficacy.


Assuntos
Antivirais/uso terapêutico , Medicamentos de Ervas Chinesas/uso terapêutico , Hepatite B Crônica/tratamento farmacológico , Cápsulas , DNA Viral/sangue , Antígenos E da Hepatite B/sangue , Humanos , Qualidade de Vida
17.
Exp Parasitol ; 204: 107726, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31299264

RESUMO

The aims of this study were to evaluate if the use of copper oxide wire particles, isolated or in association with closantel, in lambs infected with Haemonchus contortus enhances the anthelmintic efficacy of closantel, as well as to evaluate the effects of treatment in hepatic energy metabolism, inflammatory markers and hematological and biochemical tests. The lambs were randomly divided into five groups (6 animals each), as follows: uninfected animals (Control); animals infected with H. contortus (HC); infected and treated with closantel (HC + CL); infected and treated with copper oxide wire particles (HC + Cu); and infected and treated with closantel plus copper oxide wire particles (HC + CL + Cu). The animals of infected groups were infected orally with H. contortus (5,000 L3 -larvae) and on day 14 post infection (p.i) the treatments were initiated. The egg per gram of feces (EPG), butyrylcholinesterase (BuChE), myeloperoxidase (MPO), adenylate kinase (AK) and pyruvate kinase (PK) activities and hematological and biochemical tests were evaluated. Treatments with copper oxide (isolated and associated) were able to reduce the EPG count on days 28, 35, 42 and 49 p.i when compared to HC group, while closantel was able to reduce EPG only from day 35 p.i. Moreover, treatment with closantel (isolated or associated) was able to prevent the inhibition of hepatic AK and PK activities caused by H. contortus infection, which may contribute to efficient intracellular energetic communication in order to maintain the balance between cellular ATP consumption and production. Butyrylcholinesterase and MPO activities were higher in infected lambs compared to uninfected, while treated groups showed lower enzymatic activity compared to the group HC. The use of all therapeutic protocols was able to reduce the EPG count. Based on these evidences, the use of copper oxide plus closantel may be considered an alternative to treat lambs infected by H. contortus.


Assuntos
Anti-Helmínticos/administração & dosagem , Cobre/administração & dosagem , Hemoncose/veterinária , Inflamação/veterinária , Salicilanilidas/administração & dosagem , Doenças dos Ovinos/tratamento farmacológico , Abomaso/metabolismo , Adenilato Quinase/metabolismo , Administração Oral , Animais , Anti-Helmínticos/farmacologia , Anti-Helmínticos/uso terapêutico , Análise Química do Sangue/veterinária , Butirilcolinesterase/sangue , Cápsulas , Metabolismo Energético/efeitos dos fármacos , Contagem de Eritrócitos/veterinária , Fezes/parasitologia , Hemoncose/complicações , Hemoncose/tratamento farmacológico , Hemoncose/metabolismo , Hematócrito/veterinária , Hemoglobinas/análise , Inflamação/tratamento farmacológico , Inflamação/prevenção & controle , Fígado/efeitos dos fármacos , Fígado/enzimologia , Fígado/metabolismo , Masculino , Contagem de Ovos de Parasitas/veterinária , Peroxidase/sangue , Piruvato Quinase/metabolismo , Distribuição Aleatória , Salicilanilidas/farmacologia , Salicilanilidas/uso terapêutico , Ovinos , Doenças dos Ovinos/metabolismo , Doenças dos Ovinos/prevenção & controle
18.
Zhongguo Zhong Yao Za Zhi ; 44(9): 1904-1910, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31342720

RESUMO

Xixian Tongshuan Capsules with functions of promoting blood circulation and removing blood stasis,dispelling wind and resolving phlegm,relaxing muscles and activating collaterals,restoring consciousness and inducing resuscitation,has significant effects on main and concurrently symptoms of apoplexy. In this research,908 chemical compounds of Xixian Tongshuan Capsules were collected,and 337 potential targets were discovered by pharmacophore based reverse target identification. Protein interaction network( PIN)was then constructed and Identifying Protein Complex Algorithm( IPCA) was used to obtain the modules of the capsule and analyze the potential action mechanism. According to the research,Xixian Tongshuan Capsules could play a therapeutic role for hyperlipidemia and hypertension by regulating lipid metabolic process and blood pressure,the most direct risk factors of apoplexy. It could be used to treat the cerebral thrombosis and irreversible death of nerve tissue caused by insufficient supply of cerebral tissue blood and oxygen,in a way of regulating blood circulation system and nervous system. Xixian Tongshuan Capsules could also treat stroke-induced inflammation and inflammatory immune response through its regulatory effect on inflammatory immune response. Based on the network analysis,the antiinflammatory activity of Xixian Tongshuan Capsules extracts was investigated by measuring the NO release with Griess reagent method through LPS-induced in vitro inflammation model of RAW264. 7 cells. The results showed that Xixian Tongshuan Capsules extracts inhibited the secretion of NO by LPS-induced RAW264. 7 cells,indicating favorable anti-inflammatory activity. This research illuminates the mechanism of Xixian Tongshuan Capsules based on the PIN analysis at molecular network level,providing a scientific basis for its clinical application.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Inflamação/tratamento farmacológico , Acidente Vascular Cerebral/tratamento farmacológico , Animais , Cápsulas , Humanos , Camundongos , Óxido Nítrico/metabolismo , Mapas de Interação de Proteínas , Células RAW 264.7
19.
Food Chem ; 300: 125183, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31336276

RESUMO

A reasonable, high sensitive and accurate analytical method for the determination of 20 allergenic disperse dyes in foodstuffs was developed and validated. The obtained results showed that an ultra high liquid performance chromatography system - equipped with tandem quadrupole mass spectrometry (UHPLC-MS/MS) proved to be ideal for the selected method enabling multidimensional processing of the samples. Under optimized conditions, validation results showed excellent linearity (5-1000 µg/L, r2 ≥ 0.997), limits of detection (LODs, 1.1-10.8 µg/kg), recoveries (60.2-110.3%) and precision (RSDs ≤ 12.6%) for the twenty disperse dyes under investigation. The developed method was successfully applied to the analysis of 20 disperse dyes in real foodstuffs demonstrating the validity and applicability of the current method for continuing monitoring of the selected dyes. The proposed UHPLC-MS/MS is thus proved to be a convenient, effective, sensitive and timesaving method for the isolation and determination of allergenic disperse dyes in edible packaging and other foodstuffs.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Análise de Alimentos/métodos , Corantes de Alimentos/análise , Espectrometria de Massas em Tandem/métodos , Cápsulas/análise , Frutas/química , Limite de Detecção , Sensibilidade e Especificidade
20.
Expert Rev Clin Pharmacol ; 12(8): 729-739, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31282768

RESUMO

Introduction: Many women seek treatment to alleviate menopausal vasomotor symptoms (VMS). Numerous women use combination compounded hormone therapy (CHT) to achieve the benefits of estrogen/progesterone for endometrial protection. TX-001HR is a combination of bioidentical 17ß-estradiol (E2) and progesterone (P4) in a single capsule designed for continuous daily use to treat moderate to severe VMS. Areas covered: This drug profile describes the efficacy and safety of 4 doses of this E2/P4 (mg/mg: 1/100, 0.5/100, 0.5/50, 0.25/50) for treating moderate to severe VMS in menopausal woman with a uterus. Expert opinion: In REPLENISH (NCT01942668), the two highest doses of TX-001HR significantly reduced VMS frequency and severity at 4 and 12 weeks versus placebo (co-primary endpoints); all doses met the primary endpoint of endometrial safety. Rates of amenorrhea were high and improved over time; the Menopause Quality of Life and Medical Outcomes Study-Sleep instruments improved with E2/P4. TX-001HR was well tolerated and had no clinically significant impact on vital signs, metabolic or coagulation parameters, or breast safety. The combination bioidentical E2/P4 capsule (1 mg/100 mg dose was FDA-approved as Bijuva in October 2018) may provide a safe, effective, rigorously studied alternative for women with a uterus who prefer CHT for relief of VMS.


Assuntos
Estradiol/administração & dosagem , Menopausa , Progesterona/administração & dosagem , Qualidade de Vida , Administração Oral , Cápsulas , Relação Dose-Resposta a Droga , Estradiol/efeitos adversos , Estradiol/farmacologia , Feminino , Fogachos/tratamento farmacológico , Humanos , Progesterona/efeitos adversos , Progesterona/farmacologia , Índice de Gravidade de Doença , Sudorese/efeitos dos fármacos
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