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1.
Vet Parasitol ; 277: 109017, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31901535

RESUMO

Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is the complex Echinococcus granulosus sensu lato (s. l.), composed of several species/genotypes. Diagnosis in the definitive host and molecular epidemiology studies are important points for cystic echinococcosis control. Here we developed a new copro-LAMP assay, LAMP EGSL, for diagnosis in the definitive host for simultaneous detection of Echinococcus granulosus sensu stricto (s. s.), Echinococcus ortleppi, and Echinococcus canadensis species. Also, the analytical sensitivity, specificity and plausibility of performance in a rural context of a previously reported species-specific LAMP reaction, was evaluated. Both reactions showed high analytical sensitivity values (10 fg-100 fg DNA) and did not show cross reaction with DNA from host or other helminthic parasites. LAMP EGSL was performed with samples from an endemic area. In addition, the alkaline hydrolysis of one E. granulosus s. s. adult parasite followed by specific LAMP to E. granulosus s. s. was performed in a laboratory with low resources from another cystic echinococcosis endemic area. The results obtained suggest that LAMP EGSL represents a potential tool for canine diagnosis that could be useful for cystic echinococcosis control programs. In addition, we showed that LAMP reaction for E. granulous s. s., E. ortleppi and E. canadensis specific detection, could be useful for molecular epidemiology studies applicable to the definitive host. Both reactions were performed in endemic, rural areas without sophisticated equipment.


Assuntos
Testes Diagnósticos de Rotina/métodos , Doenças do Cão/diagnóstico , Equinococose/veterinária , Echinococcus granulosus , Parasitologia/métodos , Animais , Doenças do Cão/parasitologia , Cães , Equinococose/diagnóstico , Equinococose/parasitologia , Sensibilidade e Especificidade , Especificidade da Espécie
2.
Vet Parasitol ; 277: 109015, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31874403

RESUMO

Canine leishmaniosis (CanL)-associated chronic kidney disease is a leading cause of morbidity and mortality in Mediterranean countries. Novel renal biomarkers, such as serum symmetric dimethylarginine (sSDMA), may be useful surrogates for the detection of renal functional impairment. The objectives of this study were to investigate sSDMA concentrations in dogs with CanL, with and without azotemia, and to establish any potential association with the prevalence and severity of proteinuria, with the prevalence of decreased urine specific gravity and with the LeishVet clinical stages of CanL. Serum samples from 68 dogs with CanL (50 nonazotemic and 18 azotemic) and 17 healthy dogs were retrospectively examined. Increased sSDMA was documented in 26 % of dogs with CanL without azotemia and in 83.3 % of dogs with azotemia. Serum SDMA was significantly higher in azotemic compared to nonazotemic dogs and was associated with the presence and severity of proteinuria, the decreased urine specific gravity and the advanced clinical stages of CanL. The results of the present study indicate that sSDMA may be a useful adjunct to serum creatinine and urine protein/creatinine ratio for the detection of CanL-associated nephropathy, but it is of limited value for distinguishing among the LeishVet clinical stages of CanL.


Assuntos
Arginina/análogos & derivados , Doenças do Cão/diagnóstico , Leishmaniose Visceral/complicações , Leishmaniose Visceral/diagnóstico , Insuficiência Renal Crônica/etiologia , Animais , Arginina/sangue , Azotemia/veterinária , Biomarcadores/sangue , Doenças do Cão/sangue , Cães , Leishmania infantum/fisiologia , Insuficiência Renal Crônica/sangue , Estudos Retrospectivos
3.
J Enzyme Inhib Med Chem ; 35(1): 227-234, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31752563

RESUMO

A novel series of thieno[3,2-d]pyrimidine derivatives were synthesised and their inhibitory effects against diacylglycerol acyltransferase 1 (DGAT-1) were assessed. cis-Isomer 17a showed potent and selective inhibitory activity against DGAT-1 in SF9 cells. In addition, 17a had an acceptable pharmacokinetic profile and accumulated mainly in the small intestine and liver. Oral administration of 17a led to a significant reduction in plasma triacylglycerol level during an oral lipid tolerance test (OLTT) in murine and canine models. Taken together, 17a is a high-quality candidate that deserves further investigation.


Assuntos
Diacilglicerol O-Aciltransferase/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Pirimidinas/farmacologia , Animais , Diacilglicerol O-Aciltransferase/metabolismo , Cães , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Humanos , Masculino , Camundongos , Camundongos Endogâmicos ICR , Estrutura Molecular , Pirimidinas/síntese química , Pirimidinas/química , Relação Estrutura-Atividade
4.
Ultrasonics ; 101: 106033, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31561207

RESUMO

The objective of this study was to use ultrasound in combination with nanoparticulate formulations of taxane drugs for an additive approach to overcome multidrug resistance (MDR). Polymeric nanoparticulate formulations containing both chemotherapeutic taxane drugs and a polymeric inhibitor (MePEG17-b-PCL5) of drug resistant proteins have been previously developed in an attempt to overcome MDR in cells. High frequency (>1 MHz) ultrasound has been shown to increase the uptake of cytotoxic drugs in MDR proliferating cells and has been suggested as a different way to overcome MDR, resensitize drug resistant cancer cells and allow for chemotherapeutic efficacy. MDCK-MDR cells were incubated with docetaxel (DTX) or paclitaxel (PTX) loaded, solid core, nanoparticles made from a 50:50 ratio of two diblock copolymers, MePEG114-b-PCL200 and MePEG17-b-PCL5 (PCL200/PCL5). The accumulation of drug in MDCK-MDR cells was measured using radiolabeled drug and the viability of cells was determined using an MTS cell proliferation assay. The effect of ultrasound (4 MHz, 32 W/cm2, 10 s, 25% duty cycle) on drug uptake and cell viability was studied. Using free DTX or PTX, MDCK-MDR cells were killed at sublethal doses of drug with the P-gp inhibitor (MePEG17-b-PCL5) present at a concentration of just 0.006% (m/v) and cell death began after just 3 h of incubation. Using sublethal incubation doses of PTX or DTX in PCL200/PCL5 nanoparticles for 90 min, followed by a second exposure to blank PCL200/PCL5 nanoparticles, cell viability dropped by approximately 60% at 24 h. Drug accumulation increased by 1.43-1.9 fold following five bursts of ultrasound applied at 90 min. Both, increased ultrasound exposure and increased concentrations of blank nanoparticles during the second incubation allowed for increased levels of cell death. The combined use of ultrasound with taxane and P-gp inhibitor loaded polymeric nanoparticles may allow for increased accumulation of drug and inhibitor which may then release both agents inside cells in a controlled manner to overcome drug resistance in MDR cells.


Assuntos
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/antagonistas & inibidores , Antineoplásicos Fitogênicos/farmacologia , Docetaxel/farmacologia , Nanopartículas/química , Paclitaxel/farmacologia , Polietilenoglicóis/farmacologia , Ondas Ultrassônicas , Animais , Antineoplásicos Fitogênicos/química , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Docetaxel/química , Cães , Sistemas de Liberação de Medicamentos , Resistência a Múltiplos Medicamentos , Resistencia a Medicamentos Antineoplásicos , Paclitaxel/química , Polietilenoglicóis/química , Polímeros/química , Polímeros/farmacologia , Células Tumorais Cultivadas
5.
Vet Clin North Am Small Anim Pract ; 50(1): 135-153, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31635915

RESUMO

Minimally invasive plate osteosynthesis (MIPO) is a biologically friendly approach to fracture reduction and stabilization that is applicable to many radius and ulna fractures in small animals. An appropriate knowledge of the anatomy of the antebrachium and careful preoperative planning are essential. This article describes the MIPO technique, which entails stabilization of the fractured radius with a bone plate and screws that are applied without performing an extensive open surgical approach. This technique results in good outcomes, including a rapid time to union and return of function.


Assuntos
Gatos/cirurgia , Cães/cirurgia , Membro Anterior/cirurgia , Fixação Interna de Fraturas/veterinária , Fraturas Ósseas/veterinária , Procedimentos Cirúrgicos Minimamente Invasivos/veterinária , Animais , Gatos/lesões , Cães/lesões , Membro Anterior/lesões , Fixação Interna de Fraturas/métodos , Fraturas Ósseas/cirurgia
6.
Vet Clin North Am Small Anim Pract ; 50(1): 123-134, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31635917

RESUMO

A thorough knowledge of humeral anatomy is critical to performing minimally invasive techniques. Fluoroscopy, when available, is invaluable in optimizing fracture repair with minimally invasive techniques. Minimally invasive approaches decrease morbidity and allow an earlier return to function. Minimally invasive fracture repair is performed using implant systems similar to open approaches.


Assuntos
Gatos/cirurgia , Cães/cirurgia , Membro Anterior/cirurgia , Fixação Interna de Fraturas/veterinária , Fraturas Ósseas/veterinária , Procedimentos Cirúrgicos Minimamente Invasivos/veterinária , Animais , Gatos/lesões , Cães/lesões , Membro Anterior/lesões , Fixação Interna de Fraturas/métodos , Fraturas Ósseas/cirurgia
7.
Vet Clin North Am Small Anim Pract ; 50(1): 101-121, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31653537

RESUMO

This article describes the technique of percutaneous pinning in dogs and cats. Only acute fractures evaluated within the first 48 hours after trauma are selected for percutaneous pinning. Reduction is performed with careful manipulation of the fracture to minimize the trauma to the growth plate. After ensuring the fracture is reduced anatomically, smooth pins of appropriate size are inserted through stab incisions or through large-gauge needles. Depending on the anatomic location, the pins are cut flush with the bone or bent over. The main advantages of this technique are the minimal surgical trauma and lower perioperative morbidity.


Assuntos
Pinos Ortopédicos/veterinária , Gatos/cirurgia , Cães/cirurgia , Fraturas Ósseas/veterinária , Animais , Gatos/lesões , Cães/lesões , Fraturas Ósseas/cirurgia , Procedimentos Cirúrgicos Reconstrutivos
8.
J Sci Food Agric ; 100(1): 325-334, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31584699

RESUMO

BACKGROUND: Meat fraud and adulteration incidents occur frequently in almost all regions of the globe, especially with the increase in the world's population. To ensure the authenticity of meat products, we developed a 10-plex xMAP assay to simultaneously detect ten animal materials: bovine, caprine, poultry, swine, donkey, deer, horse, dog, fox and mink. RESULTS: This method was investigated by analyzing DNA extracts from raw muscle, muscle mixtures, meat products and animal feeds. Our results indicated that the species of interest can be identified, differentiated and detected down to 1 g kg-1 in binary mixtures or 0.01-0.001 ng of genomic DNA from specific species. Testing of 125 commercial samples showed a 97.4% coincidence rate with the method used in routine testing in our lab. CONCLUSION: These results indicated that the method established in this study could detect ten animal materials simultaneously within 3 h, which provides a new, useful tool for animal ingredient analysis in meat products and animal feeds. © 2019 Society of Chemical Industry.


Assuntos
DNA Mitocondrial/genética , Contaminação de Alimentos/análise , Produtos da Carne/análise , Técnicas de Amplificação de Ácido Nucleico/métodos , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Ração Animal/análise , Animais , Bovinos , Cervos , Cães , Raposas , Cabras , Cavalos , Vison , Técnicas de Amplificação de Ácido Nucleico/instrumentação , Aves Domésticas , Suínos
9.
Artigo em Inglês | MEDLINE | ID: mdl-31815976

RESUMO

The purpose of this study was to evaluate the effect that microgrooved abutments may have on peri-implant tissues. After the flapless extractions of the mandibular premolars of eight dogs, four Laser-Lok implants were placed in each mandibular quadrant, and half of them received laser-microgrooved abutments. A xenograft (MinerOss X, BioHorizons) was used in the gaps. The dogs were euthanized 12 weeks after treatment, and the histomorphometric analysis was performed. Unlike the machined abutments, the microgrooved abutments influenced the orientation of connective tissue fibers, which appeared perpendicularly and adhered to the implant-abutment surfaces, preventing the apical migration of the junctional epithelium. Laser-microgrooved abutments showed superior results.


Assuntos
Dente Suporte , Implantes Dentários , Animais , Remodelação Óssea , Implantação Dentária Endo-Óssea , Cães , Inserção Epitelial , Mandíbula
10.
Zhongguo Zhong Yao Za Zhi ; 44(20): 4529-4537, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872642

RESUMO

Ultra-fast performance liquid chromatography-mass spectrometry( UFLC-MS/MS) was used to study the anti-inflammatory active ingredient of Millettia pachyloba,6-methoxy-8,8-dimethyl-3-( 2,4,5-trimethoxyphenyl)-4 H,8 H-pyrano[2,3-f]chromen-4-one( HN-1),in liver microsomes of rats,mice,rhesus monkeys,Beagle dogs and humans metabolic stability,and compare the metabolic differences between different species. The metabolic phenotype in human liver microsomes was determined by chemical inhibitor method. Using UPLC-Q-TOF-MS/MS detection method,the in vitro metabolites of various liver microsomes were preliminarily inferred by comparing the samples incubated for 0 min and 60 min in vitro. The metabolites of HN-1 in SD rats were presumed by comparing feces,urine,plasma blanks and samples after administration. The results showed that the metabolism of HN-1 in various liver microsomes was stable,and the metabolic properties of dog and human liver microsomes were the closest. It is mainly catabolized by CYP1 A1,CYP2 D6 and CYP3 A4 isoenzymes in human liver microsomes. The metabolites of HN-1 in vitro and in vivo,including 3 in vitro metabolites and5 in vivo metabolites,were preliminarily estimated. The results laid the foundation for further pharmacological studies of HN-1.


Assuntos
Medicamentos de Ervas Chinesas , Millettia , Espectrometria de Massas em Tandem , Animais , Cromatografia Líquida de Alta Pressão , Cães , Humanos , Camundongos , Microssomos Hepáticos , Ratos , Ratos Sprague-Dawley
11.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 486-490, 2019 Oct 11.
Artigo em Chinês | MEDLINE | ID: mdl-31713376

RESUMO

OBJECTIVE: To identify the main risk factors of human cystic echinococcosis in Shiqu County, Sichuan Province, so as to provide the reference for the formulation of echinococcosis control strategies in Shiqu County. METHODS: During the period from November 2015 through June 2017, the patients with cystic echinococcosis (case group) and healthy controls (control group) were randomly sampled from Shiqu County as the study subjects. A questionnaire survey was conducted to capture the study subjects'age, gender, ethnicity, occupation, religion, lifestyle, education level, number of household dogs, bovine and sheep, and density of dog feces in the courtyard. The major risk factors of human cystic echinococcosis were identified using a logistic regression model. RESULTS: Univariate logistic regression analysis showed 7 risk factors with statistical significance between the case and control groups, and age, lifestyle, number of household bovine, number of household sheep, number of house-hold dogs, and the density of dog feces in the courtyard were included in the multivariate logistic regression model (OR = 1.026, 4.792, 1.067, 1.022, 1.709 and 1.095, respectively). CONCLUSIONS: High age, pastoral nomadic lifestyle, high number of house-hold bovine, high number of household sheep, high number of household dogs and high density of dog feces in the courtyard are strongly associated with the riks of human cystic echinococcosis in Shiqu County.


Assuntos
Doenças do Cão , Equinococose , Animais , Estudos de Casos e Controles , Bovinos , China/epidemiologia , Cães , Equinococose/epidemiologia , Fezes , Humanos , Prevalência , Fatores de Risco , Ovinos
12.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 498-503, 2019 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-31713378

RESUMO

OBJECTIVE: To investigate the prevalence and risk factors of Blastocystis hominis infections among patients with HIV/AIDS in Fuyang City, Anhui Province. METHODS: A cross-sectional study was conducted in Fuyang City, Anhui Province in 2016. The demographic and socioeconomic status, and the lifestyle and production style were collected using a questionnaire survey. B. hominis DNA was detected in subjects'stool samples using a PCR assay, and the CD4+ T lymphocyte count and HIV viral load were measured in the subjects' blood samples. The risk factors of B. hominis infections among patients with HIV/AIDS were identified using univariate and multivariate logistic regression analyses. RESULTS: A total of 398 HIV/AIDS patients were enrolled in this study, with a mean age of 49.3 years, a mean body weight of 55.9 kg and a mean height of 164.4 cm. The prevalence of B. hominis infection was 6.78% in the study subjects, and no gender- (χ2 = 1.589, P = 0.207), education level- (χ2 =0.508, P = 0.776), marital status- (χ2 = 0.419, P = 0.811) or occupation-specific prevalence (χ2 = 2.744, P = 0.615) was detected. Among the patients with HIV/AIDS, there were no significant differences in the age (t = 0.370, P = 0.712), height (t = 1.587, P =0.113), body weight (t = 0.516, P = 0.606), CD4+ T lymphocyte count (t = 1.187, P = 0.230) or HIV viral load (t = 0.193, P =0.496) between B. hominis-infected and uninfected individuals. Dinking non-tap water [OR = 6.554, 95% CI: (1.876 to 22.903)] and keeping dogs [OR = 5.895, 95% CI: (2.017 to 17.225)] were identified as risk factors for B. hominis infection in patients with HIV/AIDS. CONCLUSIONS: The prevalence of B. hominis infection is high in HIV/AIDS patients, and drinking non-tap water and keeping dogs are risk factors for B. hominis infection among HIV/AIDS patients.


Assuntos
Infecções por Blastocystis , Blastocystis hominis , Infecções por HIV , Animais , Infecções por Blastocystis/complicações , Infecções por Blastocystis/epidemiologia , Estudos Transversais , Cães , Fezes/parasitologia , Feminino , HIV , Infecções por HIV/complicações , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco
13.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 519-521, 2019 Oct 14.
Artigo em Chinês | MEDLINE | ID: mdl-31713383

RESUMO

OBJECTIVE: To understand the epidemic trend of schistosomiasis in China from 2010 to 2017 so as to provide the scientific evidence for schistosomiasis elimination. METHODS: The information of schistosomiasis control nationwide from 2010 to 2017 was collected, including the endemic of population, status of livestock control, and Oncomelania hupensis snail control. Microsoft Excel was applied for datum management and analysis. RESULTS: From 2010 to 2017, the epidemic of schistosomiasis in China dropped significantly. The decreasing amplitude of estimated number of patients nationwide was 88.46%. Seventy-one acute schistosomiasis patients were reported and 12.68% (9/71) of them were imported. The decreasing rate of cultivated cattle was 50.09%, and the accumulative number of schistosome-infected cattle was 17 239, and the average positive rate of stool examinations decreased from 1.04% to 0.000 22%. The area with snails nationwide was 373 596.18 to 363 068.95 hm2, and the new detected area with snails was 46.71 to 1 346.73 hm2. The area with schistosome-infected snails was 171.68 hm2 in 2012 and it was 9.25 hm2 in 2013. In 72 key monitoring points of 7 endemic provinces, there were 17 schistosome positive points of water body in 2010 and 6 points in 2016. There were some high risk-factors related to schistosomiasis transmission including schisto-some-infected cattle, dogs, and field rats, and the field stools, and the pasture in the area with snails in schistosomiasis monitoring points. CONCLUSIONS: The endemic status of schistosomiasis in China has dropped significantly, and the transmission level is very low. However, the infectious source and risk factors in the endemic environments have not be eliminated. Therefore, the infectious source control, health education, snail control, and transmission monitoring should be strengthened, so as to promote the progress of schistosomiasis elimination.


Assuntos
Doenças Endêmicas , Esquistossomose , Animais , Bovinos , China/epidemiologia , Cães , Doenças Endêmicas/estatística & dados numéricos , Fezes , Humanos , Ratos , Schistosoma , Esquistossomose/epidemiologia , Caramujos
14.
Zootaxa ; 4564(1): zootaxa.4564.1.6, 2019 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-31716519

RESUMO

The taxonomic status and systematic nomenclature of the Australian dingo remain contentious, resulting in decades of inconsistent applications in the scientific literature and in policy. Prompted by a recent publication calling for dingoes to be considered taxonomically as domestic dogs (Jackson et al. 2017, Zootaxa 4317, 201-224), we review the issues of the taxonomy applied to canids, and summarise the main differences between dingoes and other canids. We conclude that (1) the Australian dingo is a geographically isolated (allopatric) species from all other Canis, and is genetically, phenotypically, ecologically, and behaviourally distinct; and (2) the dingo appears largely devoid of many of the signs of domestication, including surviving largely as a wild animal in Australia for millennia. The case of defining dingo taxonomy provides a quintessential example of the disagreements between species concepts (e.g., biological, phylogenetic, ecological, morphological). Applying the biological species concept sensu stricto to the dingo as suggested by Jackson et al. (2017) and consistently across the Canidae would lead to an aggregation of all Canis populations, implying for example that dogs and wolves are the same species. Such an aggregation would have substantial implications for taxonomic clarity, biological research, and wildlife conservation. Any changes to the current nomen of the dingo (currently Canis dingo Meyer, 1793), must therefore offer a strong, evidence-based argument in favour of it being recognised as a subspecies of Canis lupus Linnaeus, 1758, or as Canis familiaris Linnaeus, 1758, and a successful application to the International Commission for Zoological Nomenclature - neither of which can be adequately supported. Although there are many species concepts, the sum of the evidence presented in this paper affirms the classification of the dingo as a distinct taxon, namely Canis dingo.


Assuntos
Canidae , Lobos , Animais , Austrália , Cães , Filogenia
15.
Zootaxa ; 4564(1): zootaxa.4564.1.7, 2019 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-31716520

RESUMO

Adopting the name Canis dingo for the Dingo to explicitly denote a species-level taxon separate from other canids was suggested by Crowther et al.  (2014) as a means to eliminate taxonomic instability and contention. However, Jackson et al.  (2017), using standard taxonomic and nomenclatural approaches and principles, called instead for continued use of the nomen C. familiaris for all domestic dogs and their derivatives, including the Dingo. (This name, C. familiaris, is applied to all dogs that derive from the domesticated version of the Gray Wolf, Canis lupus, based on nomenclatural convention.) The primary reasons for this call by Jackson et al.  (2017) were: (1) a lack of evidence to show that recognizing multiple species amongst the dog, including the Dingo and New Guinea Singing Dog, was necessary taxonomically, and (2) the principle of nomenclatural priority (the name familiaris Linnaeus, 1758, antedates dingo Meyer, 1793). Overwhelming current evidence from archaeology and genomics indicates that the Dingo is of recent origin in Australia and shares immediate ancestry with other domestic dogs as evidenced by patterns of genetic and morphological variation. Accordingly, for Smith et al.  (2019) to recognise Canis dingo as a distinct species, the onus was on them to overturn current interpretations of available archaeological, genomic, and morphological datasets and instead show that Dingoes have a deeply divergent evolutionary history that distinguishes them from other named forms of Canis (including C. lupus and its domesticated version, C. familiaris). A recent paper by Koepfli et al.  (2015) demonstrates exactly how this can be done in a compelling way within the genus Canis-by demonstrating deep evolutionary divergence between taxa, on the order of hundreds of thousands of years, using data from multiple genetic systems. Smith et al.  (2019) have not done this; instead they have misrepresented the content and conclusions of Jackson et al.  (2017), and contributed extraneous arguments that are not relevant to taxonomic decisions. Here we dissect Smith et al.  (2019), identifying misrepresentations, to show that ecological, behavioural and morphological evidence is insufficient to recognise Dingoes as a separate species from other domestic dogs. We reiterate: the correct binomial name for the taxon derived from Gray Wolves (C. lupus) by passive and active domestication, including Dingoes and other domestic dogs, is Canis familiaris. We are strongly sympathetic to arguments about the historical, ecological, cultural, or other significance of the Dingo, but these are issues that will have to be considered outside of the more narrow scope of taxonomy and nomenclature.


Assuntos
Lobos , Animais , Austrália , Cães , Nova Guiné
16.
Artigo em Inglês | MEDLINE | ID: mdl-31778390

RESUMO

Public parks are leisure environments widely used by both, adults and children, often accompained by their pets. Soil contamination of these environments by enteric viruses and intestinal parasites occurs through these animals feces. The aim of this work was to detect Carnivore protoparvovirus 1 (CPV-1) and different species of Mastadenovirus in soils samples from a park located in a medium-sized city in Brazil and evaluate the presence of helminth eggs and larvae in 18 points of a public park soil samples, as well as feces found on this site during six months. Parasitological analyzes were conducted through flotation and sedimentation techniques, and polymerase chain reaction (PCR) was used for viral detection. Of the 216 soil and 16 feces samples, 49% (106/216) and 12% (2/16) were positivefor nematodes larvae, respectively, through sedimentation techniques. Toxocara spp eggs were found in one soil sample and one feces sample, Trichuris spp eggs were found in only one feces sample and Hookworms eggs were found in four soil samples. After reconstruction work in the streets near the park, 30% (64/216) of the samples were positive for Human Mastadenovirus C (HAdV-C), 1.4% (3/216) for HAdV-E and 0.4% (1/216) for Canine Mastadenovirus A (CAdV-A). The parasitic forms found in this study have demonstrated that the contamination of the park's soil pose a threat to human and animal health. This was the first study to report the presence of HAdVs and CAdVs in soil samples.


Assuntos
Ancylostomatoidea/isolamento & purificação , Mastadenovirus/isolamento & purificação , Microbiologia do Solo , Solo/parasitologia , Toxocara/isolamento & purificação , Ancylostomatoidea/classificação , Ancylostomatoidea/genética , Animais , Cães , Fezes/parasitologia , Humanos , Mastadenovirus/classificação , Mastadenovirus/genética , Parques Recreativos , Reação em Cadeia da Polimerase em Tempo Real , Toxocara/classificação , Toxocara/genética
17.
Rev Med Liege ; 74(11): 611-615, 2019 Nov.
Artigo em Francês | MEDLINE | ID: mdl-31729851

RESUMO

Since centuries, the human clinical observation skills include our capacity to smell. However, it is well known that this particular capacity is largely outraced by our lovely dogs. Some centers around the world are actively training these dogs in order to provide a correct diagnosis for particular diseases. This clinical observation has given rise to intensive development of digital tools which are able to recognize patterns of volatile organic compounds (VOC's) in the exhaled air, but not only. It is not a single VOC which is important but an association - a specific pattern - which at the end represents the signature of a disease. This widely opens the door to applications both in the diagnostic field, as well as for follow-up of treated patients.


Assuntos
Diagnóstico , Olfato , Compostos Orgânicos Voláteis , Animais , Cães , Humanos
18.
J Vet Med Educ ; 46(4): 438-448, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31756148

RESUMO

The stress of veterinary school and the high prevalence of psychological distress among veterinary students have been well documented. Pet ownership is known to improve overall health and reduce stress among the public. Yet, for veterinary students, owning a pet (especially a dog) can offer both rewards and challenges. The academic schedule for veterinary students often comprises long hours that can make caring for a dog challenging. This study explores the area of veterinary students' dog care options and perspectives by examining two aspects of this issue: (a) currently available options, as reported by academic administrators, and (b) perceived need for these options, as reported by veterinary students. A survey of associate deans for academic affairs (n = 30) found that routine on-site kenneling options for student-owned dogs are available at eight (26.6%) veterinary schools. Simultaneously, results of a student survey (n = 768) revealed a great desire for on-campus services. Among students who did not have access to on-campus kenneling facilities, 71.5% (453 of 634) felt that creating these options would be important or very important. Across all students surveyed, 76% (581 of 764) felt it would be important to have on-site dog housing/care available. Students experience considerable stress over having to find accommodations or care for their dogs while engaged in academic activities. Thus, providing on-site boarding and care options for student-owned dogs can play an important role in both recruiting prospective veterinary students and enhancing the well-being of those currently in the program.


Assuntos
Educação em Veterinária , Propriedade , Animais de Estimação/psicologia , Faculdades de Medicina Veterinária/estatística & dados numéricos , Estudantes de Medicina/psicologia , Animais , Cães , Humanos , Estudos Prospectivos , Inquéritos e Questionários
19.
Codas ; 31(5): e20180274, 2019.
Artigo em Português, Inglês | MEDLINE | ID: mdl-31721913

RESUMO

PURPOSE: To verify the effect of dog intervention on the regular session of speech therapy for developmental stuttering in adults. METHODS: The study involved young adults and adults with developmental stuttering. The study sample was composed of eight participants, six males and two females, ranging in age from 16 to 45 years. Participants were divided into two groups: G1 - those who underwent treatment for stuttering with the presence of a dog-therapist in the therapy room and G2 - those who underwent treatment for stuttering without the presence of the dog therapist. We included a control group, G3, composed of fluent participants, matched in age and sex to G1 and G2 to control the natural variability of speech fluency. RESULTS: Comparative results between the groups indicated that the group that performed the treatment without the presence of the dog achieved better performance, evolution and efficacy rates. CONCLUSION: for the population in this study, the dog intervention on speech therapy did not improve treatment.


Assuntos
Terapia Assistida por Animais/métodos , Fonoterapia/métodos , Gagueira/terapia , Adolescente , Adulto , Animais , Cães , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Adulto Jovem
20.
Arerugi ; 68(9): 1141-1147, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31723111

RESUMO

We present a case of early childhood-onset pork-cat syndrome possibly due to sensitization by both cats and dogs. A 6-year-old girl was referred to our hospital because of repetitive episodes of urticaria when she consumed pork meat. The patient lived with a dog and the ground floor of her house was a veterinary clinic run by her veterinarian parents. Blood tests demonstrated high specific IgE (≥50UA/ml) against cat dander, dog dander, pork, Sus s 1, Fel d 2, Can f 1, Can f 2, and Can f 3. The skin prick test was positive for raw pork and beef. Western blotting analysis detected hot spots on 67-kDa proteins in pork meat and cat dander extract. Cross-reactivity between these two proteins was confirmed by an inhibition test. Furthermore, crossreactivity between pork meat and dog dander extract was also noted. Taken together, the diagnosis of porkcat syndrome was made, and both cats and dogs were suggested to have led to the sensitization. The patient was advised to only eat well-cooked pork, and has been followed thereafter without additional reactions. The previously reported cases of this syndrome developed during adolescence and young adulthood because a considerable period from the sensitization to the development cross-reactivity with pork meat is required. To our best knowledge, this is the youngest reported case of pork-cat syndrome among English and Japanese literatures. The nomenclature of this syndrome as pet animal-meat syndrome improves the understanding of the underlying pathogenesis of cross-reactivity between animal albumins and meat albumins.


Assuntos
Hipersensibilidade Alimentar/imunologia , Carne Vermelha , Alérgenos , Animais , Gatos , Bovinos , Criança , Reações Cruzadas , Cães , Feminino , Humanos , Imunoglobulina E/sangue , Testes Cutâneos , Suínos , Adulto Jovem
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