Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 3.223
Filtrar
1.
Forensic Sci Int ; 306: 110052, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31778923

RESUMO

Metabarcoding through Next Generation Sequencing (NGS) has revolutionized environmental biological studies. The availability of this technical approach has opened the opportunity for a systematic implementation of fungal metabarcoding analysis in forensics, where standardized, sensitive and reproducible protocols are highly desirable. In the present paper, a pipeline including a semi-automated molecular protocol and user-friendly bioinformatics tools are applied to several kinds of environmental samples and forensic caseworks. The identification of fungi that characterize specific environments (like Aspergillus for indoor walls, or Penicillium, Debaryomices and Wickerhamomyces for food storage) can be informative for the provenance of samples. In some situations, fungal analysis cannot allow the identification of a defined environment but seems useful to cluster samples with similar provenance. Based on these considerations, fungal analysis can be included in a wider process of non-human DNA identification in order to provide clues on sample provenance.


Assuntos
Código de Barras de DNA Taxonômico , DNA Fúngico/genética , Fungos/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Microbiologia Ambiental , Ciências Forenses , Análise de Componente Principal , Análise de Sequência de DNA , Software
2.
J Forensic Sci ; 65(1): 276-282, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31305956

RESUMO

A body of an unknown adult female was found within a shallow burial ground in Malaysia whereas the skull was exposed and visible on the ground. During autopsy examination, nine insect larvae were recovered from the interior of the human skull and subsequently preserved in 70% ethanol. The larvae were greyish in appearance, each with a posterior elongated breathing tube. A week after the autopsy, more larvae were collected at the burial site, and some of them were reared into adults. Adult specimens and larvae from the skull and from the burial site were sequenced to obtain DNA barcodes. Results showed all adult flies reared from the burial site, as well as the larvae collected from the skull were identified as Eristalinus arvorum (Fabricius, 1787) (Diptera: Syrphidae). Here, we report the colonization of E. arvorum larvae on a human corpse for the first time.


Assuntos
Dípteros/genética , Dípteros/fisiologia , Crânio/patologia , Adulto , Animais , Sepultamento , Código de Barras de DNA Taxonômico , Bases de Dados de Ácidos Nucleicos , Complexo IV da Cadeia de Transporte de Elétrons/genética , Comportamento Alimentar , Feminino , Humanos , Larva/fisiologia , Malásia , Mudanças Depois da Morte
3.
Sci Total Environ ; 702: 134704, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31726353

RESUMO

The difficulty of censusing fish diversity hampers effective management and conservation in estuarine and coastal ecosystems, especially wetland ecosystems. Improved noninvasive fish diversity monitoring programs are becoming increasingly crucial for coastal ecosystems. In this study, we investigated fish diversity and its seasonal variation in the Nansha wetland ecosystem using environmental DNA (eDNA) metabarcoding and bottom trawling, and the two approaches were compared. With the combination of the two methods, the identified fish taxa included 78 species within 60 genera and 33 families, and five nontarget taxa were only identified by eDNA metabarcoding. Compared to the two surveys, eDNA metabarcoding identified a significantly greater number of fish species per site and per season than bottom trawling (p < 0.05), with eDNA metabarcoding identifying 32.05% more fish species than bottom trawling. The overwhelming majority of the fish orders captured in the Nansha coastal wetland by bottom trawling were recovered from eDNA analysis, although certain taxa were not sampled due to limitations. Furthermore, the Whittaker index and relative abundance analysis of the two methods showed distinct differences between the sampling seasons, suggesting seasonal variations and reflecting the current or recent existence of fish species in the coastal ecosystem. Thus, our work provides more detailed seasonal data on biodiversity in the Nansha wetland of the Pearl River Estuary, which is essential for the long-term management and conservation of coastal biodiversity. Our study also adds to the evidence that the eDNA metabarcoding approach can be used in coastal environments to monitor a broad range of taxa and reflect seasonal fluctuations in fish diversity. As an emerging and transformative method, eDNA metabarcoding shows great potential for fish diversity monitoring in coastal wetland ecosystems.


Assuntos
Biodiversidade , Conservação dos Recursos Naturais/métodos , Monitoramento Ambiental/métodos , Peixes , Animais , China , Código de Barras de DNA Taxonômico , Estuários , Áreas Alagadas
4.
Acta Trop ; 201: 105220, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31618609

RESUMO

Mexico has great diversity of phlebotomine sand flies related to cases of leishmaniasis, yet few studies have dressed the molecular taxonomy of these sand fly species. The use of the cytochrome oxidase subunit 1 (COI) gene, as a DNA Barcode has facilitated the molecular identification of sand flies species worldwide. We use the DNA barcode as a useful tool for the identification of phlebotomine sand flies of the natural reserve Los Tuxtlas from Veracruz, México. A fragment of 536 bp of the COI gene was obtained from 36 individuals belonging to eight species of five genera (Dampfomyia, Lutzomyia, Psathyromyia, Psychodopygus and Brumptomyia) with coverage between 92-100%, and found similarities ranging from 93-98% with other New World phlebotomine sand flies. The NJ dendogram grouped sand flies into eight clusters according to identified species, supported by bootstrap of 97%-100%. In conclusion, all phlebotomine sand flies were correctly identified and agree with the morphological identification, also could separate genetics the isomorphic females of the genus Brumptomyia.


Assuntos
Código de Barras de DNA Taxonômico , Vetores de Doenças/classificação , Complexo IV da Cadeia de Transporte de Elétrons/genética , Leishmaniose/genética , Phlebotomus/classificação , Phlebotomus/genética , Animais , Brasil , Feminino , México
5.
Insect Sci ; 27(1): 143-158, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29873880

RESUMO

Accurate species-level identifications underpin many aspects of basic and applied biology; however, identifications can be hampered by a lack of discriminating morphological characters, taxonomic expertise or time. Molecular approaches, such as DNA "barcoding" of the cytochrome c oxidase (COI) gene, are argued to overcome these issues. However, nuclear encoding of mitochondrial genes (numts) and poor amplification success of suboptimally preserved specimens can lead to erroneous identifications. One insect group for which these molecular and morphological problems are significant are the dacine fruit flies (Diptera: Tephritidae: Dacini). We addressed these issues associated with COI barcoding in the dacines by first assessing several "universal" COI primers against public mitochondrial genome and numt sequences for dacine taxa. We then modified a set of four primers that more closely matched true dacine COI sequence and amplified two overlapping portions of the COI barcode region. Our new primers were tested alongside universal primers on a selection of dacine species, including both fresh preserved and decades-old dry specimens. Additionally, Bactrocera tryoni mitochondrial and nuclear genomes were compared to identify putative numts. Four numt clades were identified, three of which were amplified using existing universal primers. In contrast, our new primers preferentially amplified the "true" mitochondrial COI barcode in all dacine species tested. The new primers also successfully amplified partial barcodes from dry specimens for which full length barcodes were unobtainable. Thus we recommend these new primers be incorporated into the suites of primers used by diagnosticians and quarantine labs for the accurate identification of dacine species.


Assuntos
Código de Barras de DNA Taxonômico/métodos , Primers do DNA/análise , Tephritidae/classificação , Animais , Ásia Sudeste , Austrália , Sequência de Bases , Complexo IV da Cadeia de Transporte de Elétrons/análise , Proteínas de Insetos/análise , Masculino , Ilhas do Pacífico , Filogenia , Tephritidae/genética
6.
Food Chem ; 305: 125431, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31610425

RESUMO

Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products. Currently, no strategy targeting such impurities exists in enforcement laboratories. Therefore, a generic strategy of first line screening was developed to detect and identify, through PCR amplification and sequencing of the 16S-rRNA gene, the potential presence of FE producing bacteria in FE preparations. First, the specificity was verified using all microbial species reported to produce FE. Second, an in-house database, with 16S reference sequences from bacteria producing FE, was constructed for their fast identification through blast analysis. Third, the sensitivity was assessed on a spiked FE preparation. Finally, the applicability was verified using commercial FE preparations. Using straightforward PCR amplifications, Sanger sequencing and blast analysis, the proposed strategy was demonstrated to be convenient for implementation in enforcement laboratories.


Assuntos
Bactérias/isolamento & purificação , Código de Barras de DNA Taxonômico , RNA Ribossômico 16S/análise , Bactérias/genética , Bactérias/metabolismo , Manipulação de Alimentos , Reação em Cadeia da Polimerase
7.
Mem Inst Oswaldo Cruz ; 114: e190208, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800740

RESUMO

Sand fly identification is complex because it depends on the expertise of the taxonomist. The females show subtle morphological differences and the occurrence of the species complexes are usual in this taxon. Therefore, a fragment of the cytochrome c oxidase subunit I (COI) gene is used for taxon barcoding to resolve this kind of problem. This study incorporates barcode sequences, for the first time, for Evandromyia cortelezzii and Migonemyia migonei from Argentina. The nucleotide sequence divergences were estimated to generate a neighbour-joining (NJ) tree. The automatic barcode gap discovery (ABGD) approach was employed to find the barcode gaps and the operational taxonomic unit (OTU) delimitation. Other species of the subtribe were included. The frequency histogram of divergences showed a barcoding gap. The ABGD analysis identified 14 operational taxonomic units (OTUs) from 13 morphological species. Sequences of Ev. cortelezzii and Mg. migonei formed well supported clusters and were diagnosed as primary species. These sequences are useful tools for molecular identification of the sand flies of the New World.


Assuntos
Código de Barras de DNA Taxonômico , Complexo IV da Cadeia de Transporte de Elétrons/genética , Psychodidae/genética , Animais , Argentina , Sequência de Bases , Feminino , Variação Genética , Filogenia , Psychodidae/classificação , Especificidade da Espécie
8.
Huan Jing Ke Xue ; 40(9): 4052-4060, 2019 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854868

RESUMO

Eukaryotic nano-phytoplankton plays an indispensable role in energy flow, material circulation, and stability maintenance of marine ecosystems. In this research, DNA metabarcoding technology was applied to elucidate the distribution features of eukaryotic nano-phytoplankton in the Yellow Sea for the first time. The community structure and diversity of eukaryotic nano-phytoplankton were compared based mainly on amplicons of internal transcribed spacer (ITS), 18S rDNA V4 and 18S rDNA V9. Analysis of the correlation between diversity and temperature/salinity was performed as well. The following results were noted. ① Higher proportions of Chlorophyta were obtained based on the ITS region compared with other two amplicons, while the relative abundance of Dinoflagellata was larger when 18S rDNA V4 and 18S rDNA V9 regions were applied.②The abundance-based coverage estimators (ACE), Chao1, Shannon diversity, and Simpson diversity indices were significantly higher based on the amplicon of 18S rDNA V9 than those based on ITS and 18S rDNA V4. The numbers of phyla, classes, order, and families were larger when the 18S rDNA V9 region was applied.③ Based on the amplicons of ITS, 18S rDNA V4, and 18S rDNA V9, the Shannon diversity index showed a significant positive correlation with temperature (P<0.01), indicating that as the temperature increased, the diversity of the eukaryotic nano-phytoplankton increased. The tendency was opposite for salinity, however. With an increase in salinity, the Shannon diversity index decreased significantly (P<0.05). This research compared the community structure and diversity of eukaryotic nano-phytoplankton under different amplicons and will provide a scientific basis for ecological studies of eukaryotic nano-phytoplankton, monitoring of biodiversity and dynamic changes of biological resources, and sustainable development.


Assuntos
Código de Barras de DNA Taxonômico , Fitoplâncton , Biodiversidade , China , DNA Ribossômico , Ecossistema , RNA Ribossômico 18S
9.
Zhongguo Zhong Yao Za Zhi ; 44(22): 4813-4819, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872587

RESUMO

By exploring additional phylogenetic information hidden in ITS2 secondary structure,the possibility of identifying Chrysanthemum indicum and its related species with DNA barcode of ITS2 nucleic acid sequence and its structure information were discussed.The genomic DNA was extracted from 12 samples. The ITS2 fragments were amplified by PCR and sequenced bidirectionally to obtain ITS2 sequence information. 28 sequences of related species for Ch. indicum were downloaded from Gen Bank. Until all 40 ITS2 sequences were aligned,ITS2 secondary structure prediction and structure comparison were finished. Then ITS2 secondary structure information was coded. After comparing ITS2 structure information and nucleic acid information,MP phylogenetic trees were built. The results showed that the secondary structures of ITS2 shared the same structure model--a four-fingered hand. They not only have the common characteristics of ITS2 secondary structures in plants,but also have many other conservative sequences,and their overall conservativeness is high. Among all species used in this study,their ITS2 secondary structures had obvious difference. In addition,the number of mutation sites in the joint matrix compared with the nucleic acid sequences increased by nearly 90%,which greatly enriched the number of mutation sites. This method of information analysis distinguished Ch. indicum from its related species. At the same time,the support rate of the branches of evolutionary trees and the identification rate of species were significantly improved. Although there was no distinction between Ch. zawadskii and Ch. morifolium,it effectively distinguished the three species,namely,Ch. hypargyrum,Ch.oreastrum,and Ch. dichrum. Therefore,the authors suggest that the ITS2 sequence combined with its structural data information should be applied to the identification of Ch. indicum and its related species,and be widely applied to DNA barcode research.


Assuntos
Chrysanthemum , Código de Barras de DNA Taxonômico , DNA de Plantas , DNA Espaçador Ribossômico , Filogenia , Plantas
10.
Zhongguo Zhong Yao Za Zhi ; 44(22): 4837-4843, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872590

RESUMO

The combination of morphological characteristics and DNA barcodes was used to a systematic study of Hippocampus spinosissimus,laying the foundation for rapid and accurate identification for the medical seahorse species. According to the reported literature and observation on seahorse samples,the typical characteristics of the H. spinosissimus include highly developed spiny,much short nose,single or double cheeks and strongly developed spines bordering pouch. Genomic DNAs of H. spinosissimus and other related seahorse species were extracted using the TIANamp Marine Animals DNA Kit. The COⅠ and ATP6 genes were amplified and sequenced in both directions. After the verification by Blast,the GC content,intraspecific and interspecific genetic distance,and the Neighbor joining( NJ) phylogenetic trees were analyzed by MEGA 7. The lengths of the COⅠ and ATP6 genes were 649 bp and 602-603 bp,respectively,with the average GC content of 39. 96% and 35. 37%. The maximum intraspecific genetic distances in H. spinosissimus based on COⅠ and ATP were both far less than the minimum interspecific genetic distance between H. spinosissimus and other seahorses,suggesting a significant barcoding gap. NJ analysis results of COⅠ and ATP6 exhibited that all H. spinosissimus species clustered together,indicating that the two DNA barcode could identify H. spinosissimus from other seahorses accurately and quickly. In addition,H. spinosissimus shared a close genetic relationship between H. kelloggi according to the NJ tree. Furthermore,there exits three stable subgroup structure of H. spinosissimus,indicating that COⅠ and ATP6 barcodes could be applied the indicator for the geographical ecology research of H. spinosissimus. The results obtained the typical morphological and molecular identification characteristics of H. spinosissimus,which played central roles for the development of species identification. This study provides an important basis data for expanding the medical seahorse resources and ensuring the safety of clinical medicine.


Assuntos
Código de Barras de DNA Taxonômico , Smegmamorpha/genética , Animais , Composição de Bases , DNA , Filogenia
11.
Zhongguo Zhong Yao Za Zhi ; 44(20): 4419-4425, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872627

RESUMO

In order to identify the source of Citrus grandis and evaluate its quality originate from two areas comprehensively,DNA barcode was used to identify 26 samples of C. grandis. The content of naringin,rhoifolin,naringenin and apigenin was determined by UPLC method,and the color difference was numerically studied by color difference analyzer,which was related to the effective components of C. grandis. The results showed that samples was the source of C. grandis in both regions. The ITS2 sequence length was about400-500 bp,and the sequence similarity reached 99. 82%. There was only one base deletion in the two groups. There was one base A in some medicinal materials of Guangdong at 330 bp,but no base in Chongqing. The contents of naringin and rhoifolin in Chongqing samples were higher than those in Guangdong samples,and there were statistical differences between naringenin and apigenin. The chroma value showed that L*value of Guangdong was larger,a*value was smaller,L*value of Chongqing was smaller,and a*value was larger,while the b*value of both was not significantly different; The results of correlation analysis showed that naringin,rhoifolin,naringenin were positively correlated with L*,b*value,negatively correlated with a*value,and apigenin had no correlation with L*,a*,b*value. In this study,the scientific identification and evaluation of C. grandis was carried out to provide a new idea for the further study of the rapid identification and evaluation of C. grandis.


Assuntos
Citrus/genética , Apigenina , Citrus/classificação , Código de Barras de DNA Taxonômico , Medicamentos de Ervas Chinesas
12.
Zootaxa ; 4612(2): zootaxa.4612.2.2, 2019 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-31717064

RESUMO

Taxonomic status of the widely spread xiphydriid woodwasp Xiphydria camelus (Linné, 1758) was revised by examining morphology of 964 specimens and by molecular analysis using COI barcode sequences. Both morphological and molecular approaches indicated existence of three separate species masquerading in X. camelus of authors in East Asia. The three species were finally determined as X. camelus, X. eborata Konow, 1899, and a new species, X. albopicta. Xiphydria eborata is revived from synonymy with X. camelus and X. albopicta is described as a new species from Russia (Primorskij Kraj), China (Heilongjiang) and Japan (Hokkaido). Collection records are given for all the specimens examined.


Assuntos
Himenópteros , Animais , Ásia , Camelus , China , Código de Barras de DNA Taxonômico , Extremo Oriente , Japão , Federação Russa
13.
Zootaxa ; 4612(1): zootaxa.4612.1.5, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-31717080

RESUMO

Two morphologically similar species of the fungicola species group of Bradysia Winnertz, 1867 were studied in China: Bradysia chenjinae Yang, Zhang Yang, 1993 and Bradysia minorlobus Yang, Shi Huang sp. n. The morphological species concepts were supported by the DNA barcodes of COI sequences. The genetic distances of 16 Bradysia fungicola group species were analyzed and a neighbor-joining tree was constructed. The morphological characters of both Chinese species were described and illustrated.


Assuntos
Dípteros , Animais , China , Código de Barras de DNA Taxonômico , Dípteros/genética , Nematóceros
14.
Zootaxa ; 4609(3): zootaxa.4609.3.4, 2019 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-31717094

RESUMO

New Guinea is home to the world's most diverse insular frog biota, but only a small number of taxa have been included in genetically informed assessments of species diversity. Here we describe two new species of New Guinea treefrog in the genus Litoria that were first flagged during assessments of genetic diversity (DNA barcoding) and are currently only known from the holotypes. Litoria pterodactyla sp. nov. is a large green species in the Litoria graminea species complex from hill forests in Western Province, Papua New Guinea and is the third member of this group known from south of the Central Cordillera. Litoria vivissimia sp. nov. is a small, spike-nosed species from mid-montane forests on the Central Cordillera. It is morphologically very similar to Litoria pronimia, but occurs nearly 1000 m higher than any known locality for that species. More extensive genetically informed assessment of diversity in New Guinea frogs seems certain to reveal many more as-yet-unrecognised taxa in complexes of morphologically similar species.


Assuntos
Anuros , Código de Barras de DNA Taxonômico , Animais , Cor , Nova Guiné , Papua Nova Guiné
15.
Zootaxa ; 4695(4): zootaxa.4695.4.7, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31719345

RESUMO

Based upon the comparison of the 649 bp COI gene sequences, Hasora mavis Evans, 1934 is proved to be the female of a sexually dimorphic species, H. leucospila leucospila (Mabille, 1891), and thus treated as a junior subjective synonym of the latter.


Assuntos
Borboletas , Código de Barras de DNA Taxonômico , Animais , Borboletas/genética , Feminino
16.
Zootaxa ; 4686(1): zootaxa.4686.1.6, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31719502

RESUMO

An illustrated morphological description of the adult males of Tanytarsus heliomesonyctios Langton, 1999, is provided for the first time. The males were found in mountain lakes Bolshoi Darpir (Momsky District of the Republic of Sakha (Yakutia)) and Momontai (Susumansky District of the Magadan Region), located in the Kolyma River basin. Females, pupae and larvae of T. heliomesonyctios was previously described from Spitsbergen, Jan Mayen (Norway) and Ellesmere Island (Arctic Canada), and considered parthenogenetic. Tanytarsus heliomesonyctios is here for the first time noted for the fauna of Russia. Comparison of DNA barcodes shows high K2P nucleotide distances (1.7%) between the sexual populations (Norway and Russia) and the parthenogenetic populations (Svalbard and Canada). In the Bayesian tree, the COI- sequences from adult males group as sister to a strongly supported clade of sequences from parthenogenetic populations. This apparently indicates a single origin of parthenogeneticity, perhaps due to extreme environmental conditions.


Assuntos
Chironomidae , Animais , Teorema de Bayes , Canadá , Código de Barras de DNA Taxonômico , Feminino , Masculino , Noruega , Federação Russa , Svalbard
17.
Zootaxa ; 4576(1): zootaxa.4576.1.4, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31715775

RESUMO

Eleven (two known and nine new) species of the subgenus Stegana (Steganina) from China are described or redescribed: S. (S.) longifibula Takada, 1968, S. (S.) toyaensis Okada Sidorenko, 1992, S. (S.) biflava sp. nov., S. (S.) flavivittata sp. nov., S. (S.) hirtifoliacea sp. nov., S. (S.) latitabula sp. nov., S. (S.) panda sp. nov., S. (S.) pinguifoliacea sp. nov., S. (S.) spatulata sp. nov., S. (S.) stachydifolia sp. nov. and S. (S.) unguiculata sp. nov.; they are assigned into the coleoptrata, ornatipes and undulata species groups, respectively. A total of 130 DNA sequences of partial mitochondrial cytochrome c oxidase subunit I (mtCOI) gene of 38 species (including the 11 species) of above-mentioned three groups are newly obtained in this study. These sequences and other available barcoding sequences of the three groups are involved in a molecular analysis using neighbor-joining (NJ) method, in order to assess the availability of DNA barcoding for delimiting the Steganina species. The result indicates that all the sampled Steganina morphospecies within the three groups are monophyletic.


Assuntos
Drosophilidae , Animais , China , DNA , Código de Barras de DNA Taxonômico , Drosophilidae/genética , Filogenia
18.
Zootaxa ; 4678(1): zootaxa.4678.1.1, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31715979

RESUMO

A total of 50 (43 known and seven new) species in the subgenus Phortica (sensu stricto) were surveyed and (re)described from China: P. bicornuta (Chen Toda, 1997); P. bipartita (Toda Peng, 1992); P. biprotrusa (Chen Toda, 1998); P. cardua (Okada, 1977); P. chi (Toda Sidorenko, 1996); P. conifera (Okada, 1977); P. eparmata (Okada, 1977); P. eugamma (Toda Peng, 1990); P. excrescentiosa (Toda Peng, 1990); P. fangae (Máca, 1993); P. flexuosa (Zhang Gan, 1986); P. foliata (Chen Toda, 1997); P. gamma (Toda Peng, 1990); P. gigas (Okada, 1977); P. glabtabula Chen Gao, 2005; P. hainanensis (Chen Toda, 1998); P. hongae (Máca, 1993); P. huazhii Cheng Chen, 2008; P. iota (Toda Sidorenko, 1996); P. jadete Zhu, Cao Chen, 2018; P. kappa (Máca, 1977); P. lambda (Toda Peng, 1990); P. latifoliacea Chen Watabe, 2008; P. magna (Okada, 1960); P. okadai (Máca, 1977); P. omega (Okada, 1977); P. orientalis (Hendel, 1914); P. pangi Chen Wen, 2005; P. paramagna (Okada, 1971); P. perforcipata (Máca Lin, 1993); P. pi (Toda Peng, 1990); P. protrusa (Zhang Shi, 1997); P. pseudopi (Toda Peng, 1990); P. pseudotau (Toda Peng, 1990); P. psi (Zhang Gan, 1986); P. rhagolobos Chen Gao, 2008; P. saeta (Zhang Gan, 1986); P. setitabula Chen Gao, 2005; P. subradiata (Okada, 1977); P. tau (Toda Peng, 1990); P. uncinata Chen Gao, 2005; P. unipetala Chen Wen, 2005; P. allomega Gong Chen, sp. nov.; P. archikappa Gong Chen, sp. nov.; P. dianzangensis Gong Chen, sp. nov.; P. imbacilia Gong Chen, sp. nov.; P. liukuni Gong Chen, sp. nov.; P. tibeta Gong Chen, sp. nov.; and P. xianfui Gong Chen, sp. nov. In addition, seven new synonyms were recognized: P. acongruens (Zhang Shi, 1997), syn. nov.; P. antillaria (Chen Toda, 1997), syn. nov.; P. kukuanensis Máca, 2003, syn. nov.; P. linae (Máca Chen, 1993), syn. nov.; P. shillongensis (Singh Gupta, 1979), syn. nov.; P. takadai (Okada, 1977), syn. nov.; and P. watanabei (Máca Lin, 1993), syn. nov. A key to all Asian species (except for the eparmata species complex) of this subgenus was provided. All currently available DNA barcode (partial mitochondrial cytochrome c oxidase subunit I (COI) gene) sequences of this subgenus (217 sequences of 54 species) are employed in a molecular analysis using different species delimitation methods. The results indicate that approximately 68.5% (37 of 54 spp.) of Phortica (s. str.) species could be clearly distinguished from closely related morphospecies or cryptic species.


Assuntos
Código de Barras de DNA Taxonômico , Drosophilidae , Animais , China , Drosophilidae/genética , Extremo Oriente , Filogenia
19.
Zootaxa ; 4674(4): zootaxa.4674.4.2, 2019 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-31715992

RESUMO

The specimens of the family Hesperiidae collected from Tibet during 2016-2018 are identified using morphology. COI sequences of 76 individuals are newly obtained. The result of our morphological study is congruent with COI gene analyses. Maximum likehood (ML) and Bayesina inferences (BI) analyses reveal that individuals identified morphologically as the same species cluster cohesively. The minimum interspecific genetic distance is 1.7% between Halpe aucma and H. filda, and the genetic distance between conspecific individuals ranged from 0 to 0.2% for the genus Halpe. A total of 51 species are recognized, and six of them, Celaenorrhinus consanguineus Leech, 1891, Barca bicolor (Oberthür, 1896), Aeromachus propinquus Alphéraky, 1897, Pedesta bivitta (Oberthür, 1886), Baoris penicillata chapmani Evans, 1937, and Ochlodes brahma Moore, 1878, are reported from Tibet for the first time, and the last species is new to China.


Assuntos
Borboletas , Animais , Borboletas/genética , China , Código de Barras de DNA Taxonômico , Filogenia , Tibet
20.
Zootaxa ; 4674(2): zootaxa.4674.2.4, 2019 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-31716012

RESUMO

Illustrated redescription of adult male and description for the first time of pupa and fourth instar larva, as well as DNA barcoding, of Hydrobaenus laticaudus Sæther in comparison with close related known species of Hydrobaenus Fries, and an updated part of a key to species of Hydrobaenus from the Russian Far East are provided. A reference 658 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for species delimitation. For the estimation of interspecific distances and constructing detailed Bayesian tree within Hydrobaenus, we have obtained DNA barcoding for the two other species, H. distinctus and Hydrobaenus sp.1. The intraspecific sequence divergence of H. laticaudus based on the Kimura-2-parameter (K2P) distance ranged from 0.0-0.031, whereas the interspecific sequence divergence based on the K2P distance ranged from 0.106-0.197% between H. laticaudus and other species of genus Hydrobaenus. The well-supported monophyly as well as results of an ABGD analysis confirms the validity of H. laticaudus.


Assuntos
Chironomidae , Animais , Teorema de Bayes , Chironomidae/genética , Código de Barras de DNA Taxonômico , Extremo Oriente , Masculino , Filogenia , Rios , Federação Russa
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA