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1.
Zhongguo Zhong Yao Za Zhi ; 44(18): 3974-3978, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31872733

RESUMO

Callicarpa nudiflora,which is a big brand of Li nationality medicine with Hainan characteristics,has the effects of dissolving stasis,hemostasis,anti-inflammatory and antibacterial. At present,there is a lack of information about the reference genome of C. nudiflora. The study of the genome size,heterozygosity rate and characteristics of SSR of C. nudiflora,can provide an effective basis for the formulation of the whole genome de novo sequencing strategy and development of SSR molecular markers of C. nudiflora. To realize this purpose,high throughput sequencing platform Illumina Hiseq was used to sequence the genome structure of C. nudiflora and K-mer analysis was applied to estimate genome size,repeat sequences and heterozygosity rate. Simple-sequence repeat( SSR) loci that are suitable as markers were identified by MISA software. The results showed the estimated genome size of C. nudiflora was 822. 43 Mb,with a 0. 85% heterozygosity rate and 71. 67% repeats,and the GC content of genome was about 49. 20%. Therefore,C. nudiflora belongs to a complex genome with high heterozygosity and repetition. SSR molecular genetic markers were analyzed in the genome sequence,and a total of 206 049 SSRs were identified,among which mono-nucleotide,di-nucleotide and tri-nucleotide repetitive motifs summed up to 198 993,accounting for 96. 57% of the total SSRs. Among the 2-6 nucleotide repeats,AT/AT,AAT/ATT,AGCC/CTGG,AAAAT/ATTTT and AGATAT/ATATCT have the largest number,respectively. This report represents the first genome-wide characterization of C. nudiflora,and provides a reference for the construction of the library for the fine sequencing of the genome,and a molecular basis for the development of SSR molecular markers as well as for the protection and utilization of gene resources.


Assuntos
Callicarpa/genética , Genoma de Planta , Repetições de Microssatélites , Marcadores Genéticos , Polimorfismo Genético
2.
Zhongguo Zhong Yao Za Zhi ; 44(21): 4661-4669, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872662

RESUMO

The aim of this study was to clarify the main components of the green leaves of Callicarpa nudiflora,and to compare the difference of main components between the green leaves,yellow leaves,branches and seeds. In this study,ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS) coupled with the UNIFI scientific information system was adopted. And the identification of the main chemical components of C. nudiflora was combined with reference materials,literatures and online database. In addition,the difference of main components was analyzed by Progenesis QI,principal component analysis(PCA) and orthogonal partial least squares discriminant(OPLS-DA). A total of 57 compounds were identified in green leaves,including phenylpropanoids,flavonoids and iridoids. Among them,the relative content of phenylethanoid glycosides was highest. Furthermore,the PCA analysis showed that there are significant differences in main components of the branches and other parts of C. nudiflora. Combined with OPLS-DA analysis,nudifloside,parvifloroside B and ß-hydroxysamioside were selected as the characteristic components for distinguish the leaves and branches of C. nudiflora. Our study provided a scientific basis for the collection and identification of C. nudiflora.


Assuntos
Callicarpa , Espectrometria de Massas em Tandem , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Flavonoides
3.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4073-4077, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872678

RESUMO

Taking the Xiushui township of Baisha county in Hainan province as the research area,the random forest algorithm with obvious advantages in feature selection and classification extraction was used to extract the information of the Callicarpa nudiflora planting in the study area. Firstly,four kinds of different characteristic variables were generated based on World View-3 data,including spectral features,principal component features,vegetation index and texture features. Secondly,the spatial distribution of the C. nudiflora in the study area was extracted by remote sensing by random forest classification algorithm. Finally,the feature space of the random forest classification algorithm was optimized based on the feature importance to obtain the best random forest classification results,and this result is compared with the classification result of the random forest algorithm of the unoptimized feature space. The results showed that:①The overall accuracy of the C. nudiflora extracted by World View-3 image was 89. 97%,and the Kappa coefficient was 0. 84,which indicates that the random forest algorithm had higher classification accuracy and better applicability in Hainan C. nudiflora recognition.② The overall accuracy of extracting C. nudiflora with the dimension reduction feature was 90. 4,and the Kappa coefficient was 0. 85,which indicates that the random forest algorithm can effectively select features. At the same time as the feature variable data mining,the precision of the information extraction of the C. nudiflora was still guaranteed,and the operation efficiency was improved. This study provides a new idea,method and technical means for information extraction of cultivated medicinal plant resources in terms of feature selection and method selection.


Assuntos
Callicarpa , Plantas Medicinais , Algoritmos
4.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4078-4081, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872679

RESUMO

In order to solve the problem of manual area measurement,the traditional methods of medicinal planting area statistics are difficult to meet the needs of rapid area survey application. This paper uses the UAV remote sensing method with the advantages of unmanned,automatic,high efficiency,high score and short production cycle to monitor the shape of Callicarpa nudiflora. A solution for aerial photography,image data acquisition and data processing of drones were designed for characteristics and planting conditions. After data processing and statistical analysis,detailed information on the location and area of the C. nudiflora in the target area was obtained. Then the accuracy comparison analysis was carried out with the measured results of the C. nudiflora. The results show that the UAV is feasible for the monitoring of C. nudiflora,and has a good application prospect in the monitoring of Chinese herbal medicine planting.


Assuntos
Callicarpa , Plantas Medicinais , Tecnologia de Sensoriamento Remoto , Fotografação
5.
Nat Prod Res ; 33(20): 2904-2910, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30445877

RESUMO

Three new labdane-type diterpenoids, callicapene M3-M5 (1-3) were isolated from the Callicarpa macrophylla Vahl. Their structures were identified by spectroscopic method. The isolated compounds were evaluated for inhibitory activity on NO production in LPS-activated RAW 264.7 macrophage cells by using MTT assays. Compounds 1-3 showed potent inhibitory activity, with IC50 value of 48.15, 46.31 and 38.72 µM respectively.


Assuntos
Callicarpa/química , Diterpenos/isolamento & purificação , Óxido Nítrico/antagonistas & inibidores , Animais , Diterpenos/química , Diterpenos/farmacologia , Concentração Inibidora 50 , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Estrutura Molecular , Óxido Nítrico/biossíntese , Folhas de Planta/química , Células RAW 264.7
6.
Chin J Nat Med ; 16(11): 871-880, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30502769

RESUMO

Poliumoside is representative of phenylethanoid glycosides, which are widely found in many plants. Poliumoside is also regarded as the main active component of Callicarpa kwangtungensis Chun (CK), though its oral bioavailability in rat is extremely low (0.69%) and its in vivo and in vitro metabolism has not yet been systematically investigated. In the present study, an ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was employed to identify the metabolites and investigate the metabolic pathways of poliumoside in rat after oral administration 1.5 g·kg-1 of poliumoside. As a result, a total of 34 metabolites (30 from urine, 17 from plasma, and 4 from bile) and 9 possible metabolic pathways (rearrangment, reduction, hydration, hydrolyzation, dehydration, methylation, hydroxylation, acetylation, and sulfation) were proposed in vivo. The main metabolite, acteoside, was quantified after incubated with rat intestinal bacteria in vitro. In conclusion, the present study systematically explored the metabolites of poliumoside in vivo and in vitro, proposing metabolic pathways that may be significant for further metabolic studies of poliumoside.


Assuntos
Bactérias/metabolismo , Bile/química , Ácidos Cafeicos/química , Callicarpa/química , Medicamentos de Ervas Chinesas/química , Glicosídeos/química , Intestinos/microbiologia , Plasma/química , Urina/química , Administração Oral , Animais , Ácidos Cafeicos/administração & dosagem , Ácidos Cafeicos/sangue , Ácidos Cafeicos/urina , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/metabolismo , Glicosídeos/administração & dosagem , Glicosídeos/sangue , Glicosídeos/urina , Masculino , Espectrometria de Massas/métodos , Estrutura Molecular , Ratos , Ratos Sprague-Dawley
7.
Int J Biol Macromol ; 120(Pt B): 2279-2284, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30144551

RESUMO

A biopolymer membrane chitosan-collagen/organomontmorillonite loaded with Callicarpa nudiflora (CS-COL/CN-OMMT) was prepared as a wound dressing. Three composite membranes including chitosan-collagen (CS-COL), chitosan-collagen/montmorillonite (CS-COL/MMT) and chitosan-collagen/organomontmorillonite (CS-COL/OMMT) were studied from physicochemical, swelling ratio, degradation ratio in vitro and moisture permeability properties. The CS-COL/OMMT composite membrane with porous layered structure exhibited a significantly higher swelling ratio, lower degradation ratio and rather excellent moisture permeability properties than other membranes. Callicarpa nudiflora were loaded on CS-COL/OMMT composite membrane to improve antibacterial activity from 20.20 ±â€¯0.50% to 68.60 ±â€¯0.10%.


Assuntos
Antibacterianos/farmacologia , Bandagens/microbiologia , Bentonita/química , Callicarpa/química , Quitosana/química , Colágeno/química , Membranas Artificiais , Antibacterianos/química , Permeabilidade , Vapor , Cicatrização/efeitos dos fármacos
8.
J Nat Prod ; 81(4): 998-1006, 2018 04 27.
Artigo em Inglês | MEDLINE | ID: mdl-29578342

RESUMO

Species belonging to the genus Callicarpa are used traditionally in Chinese medicine for the treatment of inflammation, rheumatism, and pain. Investigation of the leaves and twigs of Callicarpa bodinieri resulted in the isolation of nine new abietane diterpenoids, bodinieric acids A-I (1-9), along with six known compounds (10-15). The structures of 1-9 were elucidated on the basis of the interpretation of their HRESIMS and NMR data and by ECD calculations. To explore the potential therapeutic target of this plant for immune-mediated disease, the inhibitory activities of the isolates obtained were determined against 13 kinase enzymes. Eight compounds exhibited moderate inhibitory effects on spleen tyrosine kinase (SYK), and the IC50 values of compounds 2 and 6 were 7.2 and 10.7 µM, respectively. In addition, a preliminary structure-activity relationship of this scaffold was analyzed with both molecular docking and a 3D-QSAR pharmacophore model.


Assuntos
/química , Callicarpa/química , Inibidores de Proteínas Quinases/química , Inibidores de Proteínas Quinases/isolamento & purificação , Baço/efeitos dos fármacos , Quinase Syk/antagonistas & inibidores , /farmacologia , Simulação de Acoplamento Molecular , Ressonância Magnética Nuclear Biomolecular/métodos , Folhas de Planta/química , Inibidores de Proteínas Quinases/farmacologia , Relação Quantitativa Estrutura-Atividade , Relação Estrutura-Atividade
9.
Phytochemistry ; 149: 31-41, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29455054

RESUMO

Nine previously undescribed seco-labdane diterpenoids, nudiflopenes A-I, were isolated from the leaves of Callicarpa nudiflora. Their structures were elucidated on the basis of extensive 1D and 2D NMR spectroscopic data analysis, and the absolute configurations of these compounds were established by the modified Mosher's method and experimental and calculated electronic circular dichroism spectra. Nudiflopenes A-I belong to the class of seco-labdane diterpenoids. All of the isolates showed inhibitory activities on lipopolysaccharide-induced nitric oxide (NO) production in murine microglial BV-2 cells. The possible mechanism of NO inhibition of some bioactive compounds was also investigated using molecular docking, which revealed interactions of bioactive compounds with the inducible nitric oxide synthase (iNOS) protein.


Assuntos
Callicarpa/química , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Folhas de Planta/química , Animais , Diterpenos/química , Medicamentos de Ervas Chinesas/química , Lipopolissacarídeos/farmacologia , Camundongos , Microglia/metabolismo , Estrutura Molecular , Óxido Nítrico/biossíntese , Óxido Nítrico Sintase Tipo II/antagonistas & inibidores , Ressonância Magnética Nuclear Biomolecular
10.
J Asian Nat Prod Res ; 20(3): 242-248, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28537085

RESUMO

Two new iridoid glycosides, callicoside C (1) and callicoside D (2), together with three known compounds (3-5), were isolated from the leaves of Callicarpa nudiflora. Their structures were established by 1D and 2D NMR spectroscopy and mass spectrometry. In an in vitro bioassay, compound 1 showed pronounced hepatoprotective activity against d-galactosamine-induced toxicity in WB-F344 rat hepatic epithelial stem-like cells.


Assuntos
Callicarpa/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Glicosídeos Iridoides/isolamento & purificação , Folhas de Planta/química , Animais , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Galactosamina/farmacologia , Glicosídeos Iridoides/química , Glicosídeos Iridoides/farmacologia , Fígado/efeitos dos fármacos , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Ratos , Ratos Endogâmicos F344
11.
Phytomedicine ; 36: 273-282, 2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-29157825

RESUMO

BACKGROUND: Platelet activation and subsequent accumulation at sites of vascular injury perform a central role in thrombus formation, which is believed to be the trigger of several cardiovascular diseases, such as atherosclerosis, myocardial infarction and strokes. In this sense, the search for agents that are capable of blocking platelets aggregation has important implications for these diseases. Callicarpa nudiflora (C. nudiflora) Hook is a traditional Chinese medicine herb for eliminating stasis to subdue swelling and hemostasis. Our previous study found several compounds extracted from this herb, including 1, 6-di-O-caffeoyl-ß-D-glucopyranoside (CGP), showed inhibitory effects on adenosine diphosphate (ADP) induced platelet aggregation. PURPOSE: The aim of current study is confirmation of the anti-platelet effects and elucidation of the probable mechanisms. METHODS: The experiments were performed on platelet rich plasma freshly isolated from SD rat. ADP, U46619 or arachidonic acid (AA) induced platelet aggregation assay were performed to evaluate the anti-platelet properties of CGP. Activated αIIbß3 integrin abundance, serotonin (5-HT) secretion, thromboxane A2 (TXA2) synthesis was determined to assess the effects of CGP on platelet activation. Furthermore, RhoA and PI3K/Akt/GSK3ß signal transduction were analyzed by Western Blotting assay. In addition, radiolabelled ligand binding assay was involved to evaluate the ability of CGP binding to thromboxane prostanoid (TP) and P2Y12 receptors. RESULTS: CGP inhibited platelet aggregation induced by ADP, U46619 and arachidonic acid (AA), significantly. Furthermore, it is also found that LGP exhibited obvious inhibitory effects on αIIbß3 integrin activation, serotonin (5-HT) secretion from granule and thromboxane A2 (TXA2) synthesis. Next, we found that CGP suppressed RhoA and PI3K/Akt/GSK3ß signal transduction. Data from radiolabelled ligand binding assay showed that CGP displayed apparent competing effects on TP and P2Y12 receptors. CONCLUSION: Collectively, the data presented here demonstrated that CGP, a natural compound from Callicarpa nudiflora Hook, inhibited the development of platelet aggregation and amplification of platelet activation. These inhibitory effects may be associated with its dual-receptor inhibition on P2Y12 and TP receptors.


Assuntos
Ácidos Cafeicos/farmacologia , Callicarpa/química , Glucosídeos/farmacologia , Ativação Plaquetária/efeitos dos fármacos , Agregação Plaquetária/efeitos dos fármacos , Receptores Purinérgicos P2/metabolismo , Receptores de Tromboxano A2 e Prostaglandina H2/metabolismo , Ácido 15-Hidroxi-11 alfa,9 alfa-(epoximetano)prosta-5,13-dienoico/farmacologia , Difosfato de Adenosina/farmacologia , Animais , Feminino , Fosfatidilinositol 3-Quinases/metabolismo , Inibidores da Agregação de Plaquetas/farmacologia , Complexo Glicoproteico GPIIb-IIIa de Plaquetas/metabolismo , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Tromboxano A2/metabolismo
12.
Biomed Pharmacother ; 95: 84-94, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28826101

RESUMO

BACKGROUND: Despite the availability of synthetic antidiabetic drugs, diabetes mellitus is still affecting millions of people with increasing rate of disease incidence and mortality throughout the world. Ethnomedicinal survey documents the traditional usefulness of Callicarpa arborea Roxb. stem bark in the management of diabetes mellitus. MATERIALS AND METHODS: In our study, hydro-alcoholic extract (HAE) of Callicarpa arborea stem bark was prepared according to WHO guidelines for herbal drugs, and screened for antidiabetic activity in streptozotocin (STZ)-induced diabetic rats. Acute oral toxicity and in vitro antioxidant activity studies along with phytochemical analyses of HAE were also carried out. RESULTS: Acute oral toxicity study indicated that HAE was safe up to a dose of 2000mg/kg body weight of rats. Results of antidiabetic activity study revealed that HAE of C. arborea stem bark possesses significant (p<0.05) hypoglycemic activity compared to normal control group in experimental rats. Histological observations of treated pancreas and liver tissues confirmed the antidiabetic efficacy of HAE. In antioxidant activity, HAE exhibited significant radical scavenging activity. CONCLUSION: From results, it can be concluded that HAE of C. arborea stem bark may have possible role as herbal antioxidants in the prevention and/or treatment of oxidative stress-induced diabetes mellitus. The antioxidant property of plant phenolic and flavonoid contents present in HAE might be responsible for the antidiabetic efficacy of C. arborea stem bark.


Assuntos
Antioxidantes/farmacologia , Callicarpa/química , Diabetes Mellitus Experimental/tratamento farmacológico , Hipoglicemiantes/farmacologia , Casca de Planta/química , Extratos Vegetais/farmacologia , Animais , Antioxidantes/química , Hipoglicemiantes/efeitos adversos , Hipoglicemiantes/química , Extratos Vegetais/efeitos adversos , Extratos Vegetais/química , Caules de Planta , Ratos , Superóxidos
13.
J Pharm Biomed Anal ; 145: 666-674, 2017 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-28800528

RESUMO

Traditional Chinese medicine (TCM) materials with closely related species are frequently fungible in clinical use. Therefore, holistic comparison of the composition in bioactive compounds is essential to evaluate whether they are equivalent in efficacy. Taking three officinal species of Callicarpa as a case, we proposed and validated a standardized strategy for the discrimination of closely related TCM materials, which focused on the extraction, profiling and multivariate statistical analysis of their biochemome. Firstly, serial liquid-liquid extractions were utilized to prepare different batches of Callicarpa biochemome, and the preparation yields were utilized for the normalization of sampling quantity prior to UHPLC-IT-MS analysis. Secondly, 34 compounds, including 19 phenylethanoid glycosides, 10 flavonoids and 5 terpenoids, were identified based on an untargeted UHPLC-IT-MS method. Thirdly, method validation of linearity, precision and stability showed that the UHPLC-IT-MS system was qualified (R2>0.995, RSD<15%) for subsequent biochemome profiling. After PCA and PLS-DA analysis, 30 marker compounds were screened and demonstrated to be of good predictability using genetic algorithm optimized support vector machines. Finally, a heatmap visualization was employed for clarifying the distribution of marker compounds, which could be helpful to determine whether the three Callicarpa species are, in fact, equivalent substitutes. This study provides a standardized biochemome profiling strategy for systemic comparison analysis of closely related TCM materials, which shows promising perspectives in tracking the supply chain of pharmaceutical suppliers.


Assuntos
Callicarpa , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas , Extração Líquido-Líquido , Medicina Tradicional Chinesa
14.
Molecules ; 22(5)2017 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-28534843

RESUMO

Three new abietane-type diterpenoids, named callicapoic acid M3 (1), callicapoic acid M4 (2) and callicapoic acid M5 (3), were isolated from the Callicarpa macrophylla Vahl. Their structures were established by spectroscopic techniques (IR, UV, MS, 1D and 2D NMR). All the isolated three compounds were evaluated for inhibitory activity on NO production in LPS-activated RAW 264.7 macrophage cells by using MTT assays. Compounds 1, 2 and 3 showed potent inhibitory activity, with inhibition rates of 34.47-40.13%.


Assuntos
/química , Anti-Inflamatórios/química , Callicarpa/química , Macrófagos/efeitos dos fármacos , Óxido Nítrico/antagonistas & inibidores , /isolamento & purificação , Animais , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Linhagem Celular , Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Macrófagos/citologia , Macrófagos/metabolismo , Espectroscopia de Ressonância Magnética , Camundongos , Óxido Nítrico/biossíntese , Extratos Vegetais/química , Folhas de Planta/química
15.
Nat Prod Res ; 31(2): 181-189, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27650129

RESUMO

Four new iridoid glycoside derivatives (1-4), along with ten known iridoid glycosides (5-14), were isolated from Callicarpa nudiflora Hook et Arn. The structure of the new iridoid glycosides was elucidated as 5″-methoxy-ampicoside (1), 6″-O-trans-caffeoylcatalpol (2), 6″-O-trans-feruloylcatalpol (3) and 3″-methoxy-agnucastoside C (4) on the basis of spectroscopic analysis. Compounds 1-11 were reported from this plant for the first time. The cytotoxic activity of the isolated compounds against human cervical carcinoma Hela cells and ovarian carcinoma HeyA8 cells was evaluated using the microculture tetrazolium assay. Compounds 4, 5, 8, 12 and 13 showed cytotoxic activity against the Hela cell line with IC50 values of 25.3, 48.1, 17.3, 38.3 and 28.2 µM, respectively. While only compound 8 showed cytotoxicity against the HeyA8 cell line, with an IC50 of 35.5 µM.


Assuntos
Callicarpa/química , Glicosídeos Iridoides/isolamento & purificação , Antineoplásicos/isolamento & purificação , Antineoplásicos/farmacologia , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Humanos , Glicosídeos Iridoides/química , Glicosídeos Iridoides/farmacologia , Estrutura Molecular , Análise Espectral
16.
Bioorg Med Chem Lett ; 27(3): 670-674, 2017 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-27989668

RESUMO

A phytochemical investigation to obtain new NO inhibitors resulted in the isolation of a new diterpenoid with a rare 9,10-seco-abietane skeleton (1) and twelve known terpenoids (2-13) from Callicarpa kwangtungensis. Their structures were elucidated on the basis of extensive 1D and 2D NMR spectroscopic data analyses, and the absolute configuration of compound 1 was established by comparison of the calculated and experimental electronic circular dichroism (ECD) spectra. The inhibitory activities on lipopolysaccharide-induced NO production in murine microglial BV-2 cells of these terpenoids were evaluated, and all of the compounds showed inhibitory effects. The following molecular docking studies showed interactions of the bioactive compounds with the iNOS protein.


Assuntos
/química , Callicarpa/química , Diterpenos/química , Óxido Nítrico/metabolismo , /isolamento & purificação , Animais , Sítios de Ligação , Callicarpa/metabolismo , Linhagem Celular , Dicroísmo Circular , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Espectroscopia de Ressonância Magnética , Camundongos , Microglia/citologia , Microglia/efeitos dos fármacos , Microglia/metabolismo , Conformação Molecular , Simulação de Acoplamento Molecular , Óxido Nítrico Sintase Tipo II/química , Óxido Nítrico Sintase Tipo II/metabolismo , Folhas de Planta/química , Folhas de Planta/metabolismo , Estrutura Terciária de Proteína
17.
Fitoterapia ; 113: 151-7, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27491749

RESUMO

A phytochemical investigation of the leaves of Callicarpa kwangtungensis led to the isolation of three new diterpenoids (1-3), callipenes A-C, and eleven known analogues (4-14). Their structures were established on the basis of extensive analysis of NMR spectroscopic data, X-ray diffraction data, and experimental and calculated electronic circular dichroism spectra. Compounds 1 and 2 are rare abietane diterpenoids possessing a peroxide bridge. All of the isolates were found to inhibit LPS-induced NO production in BV-2 cells.


Assuntos
/química , Callicarpa/química , Diterpenos/química , Óxido Nítrico/metabolismo , /isolamento & purificação , Animais , Linhagem Celular , Diterpenos/isolamento & purificação , Camundongos , Estrutura Molecular , Folhas de Planta/química
18.
J Pharm Biomed Anal ; 124: 93-103, 2016 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-26938160

RESUMO

Direct spray such as leaf spray and paper spray ionization mass spectrometry (MS) is a powerful type of ambient MS for phytochemical analysis. In this paper, direct spray MS methods to rapidly distinguish and analyze five species of Callicarpa L. have been developed. To distinguish species, leaf spray MS was employed to directly analyze leaves. A small triangular leaf sample was wetted with 15 µL of spray solvent and a high DC voltage was then simply applied to the wet leaf sample, which was positioned in front of the inlet of a mass spectrometer to produce electrospray ionization. The MS signals of phenylpropanoid glycosides, i.e. forsythiaside B, poliumoside, verbascoside in leaves could be sensitively detected. The content characteristics of the phenylpropanoid glycosides in five species including Callicarpae kwangtungensis Folium, Callicarpae macrophyllae Folium, Callicarpa nudiflora Folium, Callicarpae formosanae Folium, Callicarpa longissima Folium could be used to distinguish them, then the mass spectra of the Callicarpa L. samples were analyzed using principal component analysis(PCA) or partial least squares-discriminant analysis(PLS-DA). For the rapid semi-quantitative analysis, of phenylpropanoid glycosides in leaves, paper spray MS was employed to determine phenylpropanoid glycosides in the extracts of Callicarpa L. leaves. Ginsenoside Rg1 was selected as an internal standard (I.S.). The calibration curves were constructed through ratios of target ion abundance to I.S. ion abundance vs. concentration of targets. The linearity range was 8-250 µg/mL (R(2)=0.9947) for forsythiaside B, 9-280 µg/mL (R(2)=0.9939) for verbascoside, and 9-260 µg/mL (R(2)=0.9917) for poliumoside, respectively. The limit of detection (LOD) was 1 µg/mL, 0.5 µg/mL and 1 µg/mL for forsythiaside B, verbascoside, and poliumoside, respectively.


Assuntos
Callicarpa/química , Espectrometria de Massas por Ionização por Electrospray/métodos
19.
J Nat Med ; 70(1): 28-35, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26267810

RESUMO

Cosmetic industries focus on developing materials and resources that regulate skin pigmentation. Melanin, the major pigment in human skin, protects the skin against damage from ultraviolet light. An ethanolic extract of the leaves of Callicarpa longissima inhibits melanin production in B16F10 mouse melanoma cells by suppressing microphthalmia-associated transcription factor (MITF) gene expression. Following purification and analysis using liquid chromatography-mass spectrometry (LC-MS), NMR, and biochemical assays, carnosol was determined to be responsible for the major inhibitory effect of the C. longissima extract on melanin production. Carnosol is an oxidative product of carnosic acid, whose presence in the extract was also confirmed by an authentic reference. The carnosol and carnosic acid content in the extract was approximately 16% (w/w). These results suggest that C. longissima is a novel, useful, and attractive source of skin-whitening agents.


Assuntos
/farmacologia , Callicarpa/química , Diferenciação Celular/efeitos dos fármacos , Melaninas/biossíntese , Melanoma Experimental/metabolismo , Fator de Transcrição Associado à Microftalmia/biossíntese , Extratos Vegetais/farmacologia , /química , Animais , Linhagem Celular Tumoral , Cromatografia Líquida , Expressão Gênica/efeitos dos fármacos , Humanos , Espectrometria de Massas , Camundongos , Fator de Transcrição Associado à Microftalmia/genética , Preparações Clareadoras de Pele/farmacologia , Pigmentação da Pele/efeitos dos fármacos
20.
J Asian Nat Prod Res ; 18(3): 274-9, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26507813

RESUMO

Two new iridoid glucosides, callicoside A (1) and callicoside B (2), were isolated from the leaves of Callicarpa nudiflora. Their structures were elucidated by means of spectroscopic methods and chemical evidences. In an in vitro bioassay, compound 1 showed pronounced hepatoprotective activity against D-galactosamine-induced toxicity in WB-F344 rat hepatic epithelial stem-like cells.


Assuntos
Callicarpa/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Glucosídeos Iridoides/isolamento & purificação , Glucosídeos Iridoides/farmacologia , Fígado/efeitos dos fármacos , Animais , Medicamentos de Ervas Chinesas/química , Galactosamina/farmacologia , Glucosídeos Iridoides/química , Fígado/citologia , Estrutura Molecular , Folhas de Planta/química , Ratos , Ratos Endogâmicos F344
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