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1.
Nanotheranostics ; 6(4): 436-450, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36051856

RESUMO

Viral infection is a globally leading health issue. Annually, new lethal RNA viruses unexpectedly emerged and mutated threatening health and safety. Meanwhile, it is urgent to explore novel antiviral agents, which, however, takes years to be clinically available. Nonetheless, the development of carbon dots (CDs) in the past 20 years has exhibited their vast application potentials and revealed their promising capacity as future antiviral agents considering their versatile properties and significant antiviral responses. Thus, CDs have been widely investigated as an alternative of traditional chemotherapy for inhibiting viral infection and replication in vitro. Meanwhile, attempts to apply CDs to in vivo systems are in high demand. In this review, recent developments of CDs-based antiviral therapies are systematically summarized. Furthermore, the role of CDs in photodynamic inactivation to kill viruses or bacteria is briefly discussed.


Assuntos
Pontos Quânticos , Infecções por Vírus de RNA , Viroses , Antivirais/farmacologia , Carbono/farmacologia , Humanos
2.
Sci Rep ; 12(1): 16082, 2022 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-36167895

RESUMO

Silicon (Si) may be involved in the modification of C:N:P stoichiometry and in physiological processes, increasing sorghum growth and grain production. The objective was to evaluate the effect of Si supply on C:N:P:Si stoichiometry, physiological response, growth, and grain production of sorghum. The experiment was carried out in pots with four concentrations of Si: 0; 1.2; 2.4; and 3.6 mmol L-1 in a completely randomized design, with six replicates. Physiological attributes and dark green color index were measured and grain and biomass production were determined. Posteriorly, the plant material was ground to determine silicon (Si), carbon (C), nitrogen (N), and phosphorus (P) contents in order to analyze C:N:P:Si stoichiometry. C:Si and C:N ratios decreased at all Si concentrations applied (1.2, 2.4, and 3.6 mmol L-1) and in all plant parts studied, being lower at 3.6 mmol L-1. The lowest C:P ratios of leaves and roots were observed at 3.6 mmol L-1 Si and the lowest C:P ratio of stems was observed at 1.2 mmol L-1 Si. Si concentrations were not significant for the N:P ratio of leaves. The highest N:P ratio of stems was observed at 3.6 mmol L-1, while the lowest N:P ratio of roots was observed at 2.4 and 3.6 mmol L-1. Regardless of photosynthetic parameters, the application of 1.2 mmol L-1 Si enhanced photosynthetic rate. The application of 2.4 and 3.6 mmol L-1 enhanced stomatal conductance and dark green color index. The mass of 1000 grains was not influenced by Si applications, while Si applications at all concentrations studied (1.2, 2.4, and 3.6 mmol L-1) enhanced shoot and total dry matter, not affecting root dry matter and grain production. In conclusion, Si supply modifies C:N:P:Si stoichiometry and increases physiologic parameters, growth, development, and grain production in sorghum.


Assuntos
Silício , Sorghum , Carbono/farmacologia , Grão Comestível , Nitrogênio/farmacologia , Fósforo/farmacologia , Folhas de Planta , Silício/farmacologia
3.
J Nanobiotechnology ; 20(1): 423, 2022 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-36153528

RESUMO

Cancer often develops multidrug resistance (MDR) when cancer cells become resistant to numerous structurally and functionally different chemotherapeutic agents. MDR is considered one of the principal reasons for the failure of many forms of clinical chemotherapy. Several factors are involved in the development of MDR including increased expression of efflux transporters, the tumor microenvironment, changes in molecular targets and the activity of cancer stem cells. Recently, researchers have designed and developed a number of small molecule inhibitors and derivatives of natural compounds to overcome various mechanisms of clinical MDR. Unfortunately, most of the chemosensitizing approaches have failed in clinical trials due to non-specific interactions and adverse side effects at pharmacologically effective concentrations. Nanomedicine approaches provide an efficient drug delivery platform to overcome the limitations of conventional chemotherapy and improve therapeutic effectiveness. Multifunctional nanomaterials have been found to facilitate drug delivery by improving bioavailability and pharmacokinetics, enhancing the therapeutic efficacy of chemotherapeutic drugs to overcome MDR. In this review article, we discuss the major factors contributing to MDR and the limitations of existing chemotherapy- and nanocarrier-based drug delivery systems to overcome clinical MDR mechanisms. We critically review recent nanotechnology-based approaches to combat tumor heterogeneity, drug efflux mechanisms, DNA repair and apoptotic machineries to overcome clinical MDR. Recent successful therapies of this nature include liposomal nanoformulations, cRGDY-PEG-Cy5.5-Carbon dots and Cds/ZnS core-shell quantum dots that have been employed for the effective treatment of various cancer sub-types including small cell lung, head and neck and breast cancers.


Assuntos
Antineoplásicos , Neoplasias , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Carbono/farmacologia , Resistência a Múltiplos Medicamentos , Resistencia a Medicamentos Antineoplásicos , Humanos , Nanotecnologia , Neoplasias/metabolismo , Microambiente Tumoral
4.
Int J Biol Macromol ; 216: 799-809, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35917850

RESUMO

Food-borne carbon dots (FCDs) produced naturally during food thermal processing are one of important factors affecting human health. The FCDs will inevitably encounter blood proteins after oral administration and spontaneously form protein coronas. In this study, the interaction of three major blood proteins, including albumin, gamma globulin, and fibrinogen, with FCDs from roasted mackerel was investigated for the first time. The purpose of the research is to explore the effect of the protein corona on the biological effects of cytotoxicity and the metabolic response. The results showed that FCDs spontaneously bound to the three blood proteins, and the process involved the participation of multiple interaction forces. Three protein coronas attenuated FCD-mediated cell viability damage, oxidative stress, and mitochondrial membrane potential. Further metabolomics analysis showed that FCDs disrupted cellular carbohydrate, amino acid, and nucleotide metabolism and significantly affected the expression of six metabolic pathways in normal rat kidney cells. The protein corona alleviated the disorder of energy and substance metabolism pathways. However, the protein corona inevitably expands the range of affected metabolic responses. The results of this study are of great value in exploring the toxicity characteristics of FCDs and their protein coronas.


Assuntos
Perciformes , Coroa de Proteína , Animais , Proteínas Sanguíneas/química , Carbono/farmacologia , Sobrevivência Celular , Humanos , Coroa de Proteína/química
5.
ACS Appl Mater Interfaces ; 14(33): 37528-37539, 2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-35944155

RESUMO

Carbon dots (CDs) were synthesized with the facile hydrothermal method to produce CDs/polyvinyl alcohol (PVA) active food packaging films. The CDs had a diameter ranging from 2.01 to 5.61 nm and were well-dispersed. The effects of different concentrations of CDs on mechanical strength, water resistance, morphology, optical, and thermal performance of the CDs/PVA films were discussed. The incorporation of CDs in the PVA film improved its mechanical properties, water resistance properties, UV blocking properties, and thermal stability and endowed the composite film with antioxidant and antimicrobial properties. The maximum scavenging rates of 2,2-diphenyl-1-picrylhydrazyl and ABTS free radicals by the 0.50% CDs/PVA film were 72.81 and 97.08%, respectively. The inhibition zone diameters of the 0.50% CDs/PVA solution against Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis), and Escherichia coli (E. coli) were 9.52, 8.21, and 9.05 mm, respectively. Using the 0.50% CDs/PVA film as active packaging, the shelf life of banana, jujube, and fried meatballs was observed to be extended significantly. These results demonstrate the viability of the CDs/PVA composite film as a promising active food packaging material.


Assuntos
Álcool de Polivinil , Staphylococcus aureus , Carbono/farmacologia , Escherichia coli , Embalagem de Alimentos , Conservação de Alimentos , Água
6.
Biomater Adv ; 137: 212837, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35929242

RESUMO

The herbicide and viologen, N, N'-dimethyl-4,4'-bipyridinium dichloride (Paraquat) is known to be toxic to neuronal cells by a multifactorial process involving an elevation in the levels of reactive oxygen species (ROS), the triggering of amyloid-protein aggregation and their accumulation, collectively leading to neuronal dyshomeostasis. We demonstrate that green-chemistry-synthesized sustainable gelatin-derived carbon quantum dots (CQDs) mitigate paraquat-induced neurotoxic outcomes and resultant compromise in organismal mortality. Gelatin-derived CQDs were found to possess antioxidant properties and ameliorated ROS elevation in paraquat-insulted neuroblastoma-derived SHSY-5Y cells, protecting them from herbicide-induced cell death. These CQDs also increased lifespan in paraquat-compromised Caenorhabditis elegans and herbicide-mediated dopamine neuron ablation. Collectively, the data underscore the ability of this sustainably synthesized, environmentally friendly biocompatible nanomaterial to protect cell lines and organisms against neurotoxic outcomes. The study findings strategically position this relatively novel nanoscopic carbon quantum framework for further testing in vertebrate trials of neurotoxic insult.


Assuntos
Herbicidas , Síndromes Neurotóxicas , Pontos Quânticos , Carbono/farmacologia , Gelatina , Herbicidas/toxicidade , Humanos , Síndromes Neurotóxicas/etiologia , Paraquat/toxicidade , Pontos Quânticos/toxicidade , Espécies Reativas de Oxigênio
7.
J Nanobiotechnology ; 20(1): 368, 2022 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-35953858

RESUMO

Developing highly efficient pharmaceuticals to eradicate pathogens and facilitate wound healing is of great concern. Despite some cationic carbon dots (CDs) have been used for sterilization, hardly any anionic CDs with antimicrobial activity have appeared. In the present work, we engineered a string of anionic CDs (especially CD31) as valid broad-spectrum bactericides to kill bacteria. Furthermore, CD31 conjugated with ɛ-polylysine (Plys) to construct injectable, and self-healing hydrogel (CD-Plys) that possess the advantages of remarkable broad spectrum antibacterial activity, excellent wound healing ability and satisfied biocompatibility. CD-Plys could dramatically accelerate wound healing with epithelization and enhanced angiogenesis. Taken together, this work provides a two-pronged strategy to explore CDs-based antimicrobial agents for disease therapy and tissue engineering.


Assuntos
Hidrogéis , Polilisina , Antibacterianos/farmacologia , Bactérias , Carbono/farmacologia , Hidrogéis/farmacologia , Cicatrização
8.
Anal Methods ; 14(34): 3289-3298, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35968579

RESUMO

The first-ever neomycin antibiotic-based carbon dots (Neo-CDs) were synthesized via a low-cost, eco-friendly, and single-step hydrothermal method using neomycin as a single precursor. The as-prepared Neo-CDs exhibited strong and stable blue fluorescence and were well characterized by TEM, UV-vis absorption, fluorescence emission, IR, XRD, Raman and XPS spectroscopy methods. The Neo-CDs showed a well-distributed size within the range of 4.5 to 7.8 nm, comprising various functional groups on the surface of the carbon core. The Neo-CDs exhibited exceptional emission behaviour, and fluorescence quantum yield was calculated to be 55% in double distilled water. Neo-CDs have been used as a fluorescent sensor for selective and sensitive detection of Fe3+ ions in aqueous solution in the fluorescence turn-off mode. From the set of metal ions, only the Fe3+ ion showed quenching of fluorescence due to photoinduced (PET) electron transfer from Neo-CDs to the half-filled 3d orbital of Fe3+ ions. The limit of detection for Fe3+ ions was calculated to be 0.854 µM. Further, the quenching efficiency and Stern-Volmer quenching constant have been calculated which are about 94% and 5.6 × 106 M-1, respectively.


Assuntos
Carbono , Pontos Quânticos , Carbono/química , Carbono/farmacologia , Corantes Fluorescentes/química , Íons/química , Neomicina , Pontos Quânticos/química
9.
Bioprocess Biosyst Eng ; 45(10): 1673-1682, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35964262

RESUMO

Polymalic acid (PMA) is a water-soluble polyester produced by Aureobasidium pullulans. In this study, the physiological response of A. pullulans after the addition of vegetable oils was investigated. Soybean oil (SBO) is pivotal for shortening fermentation time and achieving high PMA titer. With the addition of 1% (w/v) SBO, the titer and productivity of PMA was, respectively, increased by 34.2% and 80%. SBO acted as a chemical stimulatory agent rather than a carbon source, the enhancement on PMA production was attributed to the component of fatty acid. SBO induced the dimorphism (yeast-like cells and mycelia) of A. pullulans, in vitro enzyme activities indicated that the TCA oxidative branch for malic acid synthesis might be strengthened, which could generate more ATP for PMA synthesis, and the assay of intracellular energy supply validated this deduction. This study provided a new sight for recognizing the regulatory behavior of SBO in A. pullulans.


Assuntos
Ascomicetos , Óleo de Soja , Trifosfato de Adenosina , Aureobasidium , Carbono/farmacologia , Ácidos Graxos , Fermentação , Malatos/farmacologia , Poliésteres , Polímeros , Óleo de Soja/farmacologia , Água
10.
Chemosphere ; 307(Pt 3): 135836, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35973491

RESUMO

Environmental microplastic accumulation can lead to a range of problems and the potential loss of ecosystem services. However, the impacts of microplastics on sediment environmental properties and microbial ecosystems remain unclear. Therefore, this article aimed to better elucidate the effects of microplastics on sediment physicochemical properties, enzymatic activities and the diversity and composition of sediment microbial communities. We conducted a 60-day sediment-incubation experiment using sediments with three concentrations (2%, 5%, and 10%, w/w) and two types (polyethylene (PE) and polyvinyl chloride (PVC)) of microplastics (550 µm) in a climate-controlled chamber. The addition of both PE and PVC microplastics reduced sediment catalase, polyphenol oxidase (PO), and urease activities, and decreased physicochemical indicators, including total organic carbon (TOC), total nitrogen (TN), and pH value. In addition, microplastics reduced bacterial community diversity and inhibited the recovery of community richness, implying that microplastics may compete with sediment microorganisms for niches. Stronger effects were generally detected under a high microplastic concentration (10%), reflecting the influence of the concentration of microplastic exposure on the bacterial community. The dominant bacterial phyla were Chloroflexi, Proteobacteria, Bacteroidota, Firmicutes, and Actinobacteriota, and the relative abundances of Proteobacteria, and Bacteroidota significantly increased in the microplastic treatments during the late incubation period, indicating that microplastic addition may have improved nutritional conditions later in the incubation process. Structural equation modeling indicated that shifts in PO activity had a significant correlation with changes in Proteobacteria abundance (P < 0.01), and important drivers affecting the dissimilarity of Bacteroidota abundance were the changes of TN content and catalase activity (P < 0.05). These results indicated that microplastics with diverse characteristics affected the environmental properties of the sediment, while the physicochemical properties and enzymatic activity of the sediment could directly or indirectly exert different impacts on the dominant bacteria. This study can provide a theoretical basis for the ecological effects of microplastic contamination.


Assuntos
Microbiota , Poluentes Químicos da Água , Bactérias , Carbono/farmacologia , Catalase , Catecol Oxidase/farmacologia , Monitoramento Ambiental , Microplásticos/toxicidade , Nitrogênio/farmacologia , Plásticos/toxicidade , Polietileno/toxicidade , Cloreto de Polivinila , Urease , Poluentes Químicos da Água/análise
11.
Int J Nanomedicine ; 17: 2907-2924, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35814612

RESUMO

Background: After the synthesis of selenium doped carbon quantum dots (Se/CDs) via a step-by-step hydrothermal synthesis method with diphenyl diselenide (DPDSe) as precursor, the beneficial effects of Se/CDs' supplementation on the in vitro development competence of ovine oocytes were firstly investigated in this study by the assay of maturation rate, cortical granules' (CGs) dynamics, mitochondrial activity, reactive oxygen species (ROS) production, epigenetic modification, transcript profile, and embryonic development competence. Results: The results showed that the Se/CDs' supplementation during the in vitro maturation (IVM) process not only enhanced the maturation rate, CGs' dynamics, mitochondrial activity and embryonic developmental competence of ovine oocytes, but remarkably decreased the ROS production level of ovine oocytes. In addition, the expression levels of H3K9me3 and H3K27me3 in the ovine oocytes were significantly up-regulated after the Se/CDs' supplementation, in consistent with the expression levels of 5mC and 5hmC. Moreover, 2994 up-regulated differentially expressed genes (DEGs) and 846 repressed DEGs were found in the oocytes after the Se/CDs' supplementation. According to the analyses of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), these DEGs induced by the Se/CDs' supplementation were positively related to the progesterone mediated oocyte maturation and mitochondrial functions. And these remarkably up-regulated expression levels of DEGs related to oocyte maturation, mitochondrial function, and epigenetic modification induced by the Se/CDs' supplementation further confirmed the beneficial effect of Se/CDs' supplementation on the in vitro development competence of ovine oocytes. Conclusion: The Se/CDs prepared in our study significantly promoted the in vitro development competence of ovine oocytes, benefiting the extended research about the potential applications of Se/CDs in mammalian breeding technologies.


Assuntos
Pontos Quânticos , Selênio , Animais , Carbono/farmacologia , Suplementos Nutricionais , Desenvolvimento Embrionário , Feminino , Técnicas de Maturação in Vitro de Oócitos/métodos , Mamíferos , Oócitos/metabolismo , Gravidez , Espécies Reativas de Oxigênio/metabolismo , Selênio/metabolismo , Selênio/farmacologia , Ovinos
12.
Photodiagnosis Photodyn Ther ; 39: 103033, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35905831

RESUMO

Due to their excellent photophysical properties, carbon quantum dots have great potential in biomedical and drug delivery fields. In this study, nitrogen-doped carbon quantum dots with good water solubility were prepared using citric acid and ethylenediamine as precursors, and compounded with curcumin, a photosensitive component, to produce composite nanomaterials with photodynamic therapy and synergistic photothermal therapy. The formation of nitrogen-doped carbon quantum dots and composite nanomaterials was verified using physical and optical means. In addition, the composite nanomaterials produced single-linear oxygen and exacerbated the increase of solution temperature under blue (405 nm) and near-infrared (808 nm) light irradiation, respectively. The plate counting method showed that the composite nanomaterials exhibited good photodynamic synergistic photothermal antibacterial properties against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus under dual light source (405+808 nm) irradiation, which improved the photoinactivation of curcumin against bacteria. In addition, the composite nanomaterials exhibited low toxicity and good hemocompatibility. These experimental results suggested that the composite nanomaterials showed great potential in a multimodal photodynamic therapy synergistic photothermal treatment platform.


Assuntos
Curcumina , Nanocompostos , Fotoquimioterapia , Antibacterianos/farmacologia , Carbono/farmacologia , Curcumina/farmacologia , Nitrogênio , Fotoquimioterapia/métodos , Terapia Fototérmica
13.
Bioresour Technol ; 360: 127563, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35788386

RESUMO

Exploring the interaction between denitrifying microbial species is significant for improving denitrification performance. In this study, the effects of co-culturing fungus Penicillium citrinum and strain Citrobacter freundii on denitrification were investigated. Results showed that the maximum nitrate removal and carbon utilization in co-culture were 68.0 and 14.1 mg·L-1·d-1, respectively. The total adenosine triphosphatase activity was increased to 9.87 U‧mg-1 protein in co-culture, and nicotinamide adenine dinucleotide production was 1.7-2.3 times that of monoculture, attributing to increased carbon utilization. Further metabolomics and membrane permeability assay revealed that co-culture increased the metabolism of glycerides, thereby enhancing the membrane permeability of strain Citrobacter freundii and promoting the transmembrane transport of nitrate and glucose, which boosted nitrate reductase activity and nicotinamide adenine dinucleotide production in turn. In summary, co-culturing promoted carbon utilization and enhanced substrate removal efficiency through the metabolism of glycerides, which provided a strategy to enhance denitrification performance in wastewater treatment.


Assuntos
Desnitrificação , Nitratos , Reatores Biológicos/microbiologia , Carbono/farmacologia , Citrobacter freundii , Técnicas de Cocultura , Fungos/metabolismo , Glicerídeos/farmacologia , NAD/metabolismo , Nitratos/metabolismo , Nitrogênio/farmacologia , Óxidos de Nitrogênio , Penicillium
14.
ACS Biomater Sci Eng ; 8(8): 3608-3622, 2022 08 08.
Artigo em Inglês | MEDLINE | ID: mdl-35892286

RESUMO

In this work, a series of mesoporous carbon nanocapsules (mCNS) of size below 10 nm have been prepared from Azadirachta indica seeds with a very easy and cost-effective approach. These nanocapsules can emit red and green light and are effective for cell imaging. Further, these carbon nanocapsules are biocompatible toward the normal healthy cells, however, they possess modest cytotoxicity against the MCF-7 (human breast cancer) and triple-negative breast cancer (TNBC) (MDA- MB-231 breast cancer cells), and the rate of killing cancer cells strongly depends on the dose of mCNCs. Further, the mitochondrial membrane potential and apoptosis assay were performed to analyze the therapeutic significance of these nanocapsules to kill breast cancer. Results showed that these carbon nanocapsules can depolarize the mitochondrial membrane potential alone (without using conventional drugs) and can change the physiological parameters and cellular metabolic energy of the cancer cells and kill them. The apoptosis results confirmed the death of breast cancer cells in the form of apoptosis and necrosis. Moreover, the results suggested that the porous carbon nanocapsules (mCNCs) reported herein can be used as a potential candidate and useful for the theranostic applications such as for cancer cell detection and therapy without using any conventional drugs.


Assuntos
Azadirachta , Nanocápsulas , Neoplasias de Mama Triplo Negativas , Carbono/farmacologia , Carbono/uso terapêutico , Linhagem Celular Tumoral , Humanos , Potencial da Membrana Mitocondrial , Nanocápsulas/uso terapêutico , Neoplasias de Mama Triplo Negativas/tratamento farmacológico
15.
J Nanobiotechnology ; 20(1): 331, 2022 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-35842638

RESUMO

BACKGROUND: Food-borne carbon dots (CDs) are widely generated during food processing and are inevitably ingested by humans causing toxicity. However, the toxic effects of food-borne CDs on the blood glucose metabolism are unknown. RESULTS: In this study, we brewed beer via a representative strategy and extracted the melting-barley CDs (MBCDs) to explore the toxic effects on blood glucose in mice. We found the accumulation of fluorescent labeled MBCDs in various organs and oral administration of MBCDs can cause visceral toxicity, manifested as liver damage. Mice were orally administered MBCDs (5 and 25 mg/kg) for 16 weeks, and increased levels of fasting blood glucose were observed in both MBCDs-treated groups. Transcriptomic analyses revealed that MBCDs activate oxidative stress, inflammatory responses, the MAPK cascade, and PI3K/Akt signaling in mice livers. Mechanistically, MBCDs exposure-induced reactive oxygen species (ROS) overproduction activates the nuclear factor-κB (NF-κB) signaling pathway and MAPK cascade, thereby promoting phosphorylated insulin receptor substrate (IRS)-1 at Ser307 and inducing insulin resistance (IR). Meanwhile, the IR promoted gluconeogenesis, which enhanced MBCDs-induced hyperglycemia of mice. Importantly, inhibition of the ROS significantly attenuated the MBCDs-induced inflammatory response and MAPK cascade, thereby alleviating IR and hyperglycemia in mice. CONCLUSION: In summary, this study revealed that MBCDs promote ROS overproduction and thus induced IR, resulting in imbalance of glucose homeostasis in mice. More importantly, this study was further assessed to reveal an imperative emphasis on the reevaluation of dietary and environmental CDs exposure, and has important implications for T2DM prevention research.


Assuntos
Hordeum , Hiperglicemia , Resistência à Insulina , Animais , Glicemia/metabolismo , Carbono/farmacologia , Hordeum/metabolismo , Humanos , Hiperglicemia/metabolismo , Insulina/farmacologia , Camundongos , NF-kappa B/metabolismo , Estresse Oxidativo , Fosfatidilinositol 3-Quinases/metabolismo , Espécies Reativas de Oxigênio/metabolismo
16.
Int J Mol Sci ; 23(15)2022 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-35897812

RESUMO

The high mortality rate caused by atherosclerosis makes it necessary to constantly search for new and better treatments. In previous reports, chemically modified carbon-coated iron nanoparticles (Fe@C NPs) have been demonstrated a high biocompatibility and promising anti-plaque properties. To further investigate these effects, the interaction of these nanoparticles with the adipose tissue of Wistar rats (in vivo) and human atherosclerotic plaques (ex vivo) was studied. For the in vivo study, cobalt-chromium (CoCr) alloy tubes, which are used for coronary stent manufacturing, were prepared with a coating of polylactic acid (PLA) which contained either modified or non-modified Fe@C NPs in a 5% by weight concentration. The tubes were implanted into an area of subcutaneous fat in Wistar rats, where changes in the histological structure and functional properties of the surrounding tissue were observed in the case of coatings modified with Fe@C NPs. For the ex vivo study, freshly explanted human atherosclerotic plaques were treated in the physiological solution with doses of modified Fe@C NPs, with mass equal to 5% or 25% relative to the plaques. This treatment resulted in the release of cholesterol-like compounds from the surface of the plaques into the solution, thus proving a pronounced destructive effect on the plaque structure. Chemically modified Fe@C NPs, when used as an anti-atherosclerosis agent, were able to activate the activity of macrophages, which could lead to the destruction of atherosclerotic plaques structures. These findings could prove the fabrication of next-generation vascular stents with built-in anti-atherosclerotic agents.


Assuntos
Aterosclerose , Nanopartículas , Placa Aterosclerótica , Tecido Adiposo/patologia , Animais , Aterosclerose/tratamento farmacológico , Aterosclerose/patologia , Carbono/farmacologia , Carbono/uso terapêutico , Humanos , Ferro/uso terapêutico , Nanopartículas/química , Placa Aterosclerótica/tratamento farmacológico , Placa Aterosclerótica/patologia , Ratos , Ratos Wistar
17.
Biomater Adv ; 139: 212995, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35882144

RESUMO

The blood-brain barrier (BBB) is the major obstacle limiting the reach of therapeutic drugs into the brain. Herein, an aspirin-based anti-inflammatory replenisher (aspCD) was fabricated by carbonizing aspirin to deliver drugs into the brain visually. The as-prepared aspCD combined the BBB-penetrating and anti-inflammatory effects of aspirin with the fluorescent and drug-loading properties of carbon dots (CDs), thereby delivering therapeutic drugs into the brain and acting as imaging agent as well as anti-inflammatory replenisher. In vivo experiments of mice and zebrafish revealed that fluorescence aspCD could effectively penetrate BBB. In vitro and in vivo inflammatory models demonstrated that aspCD could be regarded as an excellent anti-inflammatory replenisher. In addition, as a functional carrier, aspCD was proved to be capable of loading drugs with different polarity. In summary, carbonization of active precursors (therapeutic drugs) into CDs could be a promising strategy to achieve the loading and visualization of drugs as well as the retainment of their biological activities.


Assuntos
Barreira Hematoencefálica , Carbono , Animais , Anti-Inflamatórios/farmacologia , Aspirina/farmacologia , Carbono/farmacologia , Peixe-Zebra
18.
Biomater Adv ; 139: 212978, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35891599

RESUMO

The pharmacological effects of curcumin can be ascribed to its dose-dependent activity. Therapeutic application of curcumin is hindered by its poor solubility and low bioavailability. Carbon dots are gaining attention in biomedical applications in view of their unique photo-physical properties. Some carbon dots derived from bioactive molecules have shown superior activity than the parent compound. With an aim to address the limitations of curcumin, herein we compared the wound healing activity of curcumin-derived carbon dots (CurCD) with curcumin. The improved solubility and stability of CurCD, combined with its superior proliferative, proangiogenic and anti-bacterial activity suggested that CurCD would be more beneficial than curcumin in wound healing. To enable the sustained release of CurCD at the wound site, a protease-responsive hydrogel (GHCD) was prepared with CurCD acting as a cross-linker. A comparative study using a skin excision model revealed that GHCD supported faster wound closure with improved angiogenesis and complete restoration of the epithelium. Apart from the establishment of CurCD as a wound healing agent, the study provides a novel carbon dot based approach for molecules with limitations of solubility and bioavailability.


Assuntos
Curcumina , Carbono/farmacologia , Curcumina/farmacologia , Hidrogéis/farmacologia , Peptídeo Hidrolases/farmacologia , Cicatrização
19.
Nanoscale ; 14(31): 11359-11368, 2022 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-35894806

RESUMO

Drug-derived carbon dots (CDs) not only have excellent photoluminescence properties of CDs, but also maintain pharmacological effects of original drugs, so as to realize extended applications for both bioimaging and chemotherapy. In this work, metformin (Met)-derived CDs (Met-CDs) as multifunctional nanocarriers with tumor cell imaging and cancer therapy are synthesized using Met and citric acid as precursors. The created Met-CDs exhibit obvious resistance to photobleaching, significant pH sensitivity in acidic environments, good pH stability in alkaline environments, and high temperature sensitivity. In addition, we further investigate the biological activity of Met-CDs using diabetic cell models, which demonstrate the ability of Met-CDs to treat diabetes and reduce the production of reactive oxygen species in diseased cells. Subsequently, human alveolar adenocarcinoma basal epithelial cells (A549) are cultured in both normal glucose and low glucose media, and different concentrations of Met and Met-CDs are added to investigate the effect of Met-CDs on A549 cells. Finally, we successfully utilize the prepared Met-CDs to image live A549 cells in vitro in normal glucose medium. The Met-CDs prepared in this work reveal high potential to be used as both fluorescent probes and drug agents for tumor therapy, realizing controllable integrated diagnosis and treatment of diseases.


Assuntos
Carbono , Pontos Quânticos , Carbono/química , Carbono/farmacologia , Corantes Fluorescentes/química , Glucose , Humanos , Preparações Farmacêuticas , Pontos Quânticos/química
20.
Chemosphere ; 305: 135496, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35764114

RESUMO

Recently, increasing attention has been paid to the inactivation of antibiotic resistant bacteria (ARB) during the electrochemical disinfection. However, no available information could be found on ARB inactivation in water during metal-free electrochemical disinfection. In this study, polyvinylidene fluoride (PVDF)-based carbon catalyst (PPC) was chosen as working electrode. Batch experiments were conducted to investigate key design for ARB inactivation, effects of water matrix and potential risks after the disinfection under the pre-determined conditions. The disinfection with current density at 2.25 mA/cm2 and Air/Water ratio of 10:1 was optimal with the largest ARB inactivation (5.0 log reduction for 40 min), which was in line with the profile and yield of hydrogen peroxide (H2O2) during the disinfection. Effects of water matrix analysis implied that ARB inactivation efficiencies during the disinfection in acidic solutions were better than the one in alkaline solutions, which could be due to rich CC levels on surface of PPC cathode. After the optimal disinfection, ARB counts increased slightly at the first 2 h and then tended to disappear, and there were no conjugation transfer and little transformation for target antibiotic resistance genes, indicating that potential risks could be blocked after the disinfection for 40 min. Furthermore, intermittent flow was more effective in inactivating ARB compared with continuous flow. These suggested that the application of metal-free electrochemical disinfection with PPC to inactivate ARB in water was feasible and desirable in this study.


Assuntos
Desinfecção , Purificação da Água , Antagonistas de Receptores de Angiotensina/farmacologia , Inibidores da Enzima Conversora de Angiotensina/farmacologia , Antibacterianos/farmacologia , Bactérias , Carbono/farmacologia , Peróxido de Hidrogênio/farmacologia , Águas Residuárias/microbiologia , Água/farmacologia
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