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1.
Molecules ; 26(4)2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33671275

RESUMO

Oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) have long been known for their organoleptic properties. Both plants are widely used in cuisine worldwide in fresh and dried form and as a pharmaceutical raw material. The study aimed to assess if the type of cultivation influenced chosen chemical parameters (total polyphenols by Folin-Ciocalteu method; carotenoids and chlorophyll content by Lichtenthaler method), antimicrobial activity (with chosen reference microbial strains) and shaped cytotoxicity (with L929 mouse fibroblasts cell line) in water macerates of dry oregano and thyme. Polyphenols content and antimicrobial activity were higher in water macerates obtained from conventional cultivation (independently from herb species), unlike the pigments in a higher amount in macerates from organic herbs cultivation. Among all tested macerates stronger antimicrobial properties (effective in inhibiting the growth of Pseudomonas aeruginosa, Bacillus cereus and Salmonella enteritidis) and higher cytotoxicity (abilities to diminish the growth of L929 fibroblasts cytotoxicity) characterized the conventionally cultivated thyme macerate.


Assuntos
Agricultura , Carotenoides/análise , Clorofila/análise , Fenóis/análise , Água/química , Animais , Anti-Infecciosos/farmacologia , Bactérias/efeitos dos fármacos , Morte Celular/efeitos dos fármacos , Linhagem Celular , Camundongos , Testes de Sensibilidade Microbiana , Origanum/química , Extratos Vegetais , Polifenóis/análise , Thymus (Planta)/química
2.
Food Chem ; 349: 129156, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-33581431

RESUMO

This study focused on the identification (by LC-PDA-qTof-ESI-MS) and quantification (by UPLC-PDA) of isoprenoids of the fruit tree leaves (FTL) of commonly consumed fruits: apple, pears, quince, apricot, peach, plums, sweet and sour cherry. The FTL were collected at 2 time points: after tree blooming and after fruit collection. In FTL 7 carotenoids and 16 chlorophylls were identified, but the number of labeled chlorophyll compounds depended on the species. FTL of apple, sour cherry and apricot were identified as the best sources of chlorophylls (mean 404.8, 388.7 and 364.5 mg/100 g dw, respectively) and sweet and sour cherry leaves as the best sources of carotenoids (831.4 and 1162.0 mg/100 g dw, respectively). A lower content of chlorophylls and carotenoids, but not significantly, was detected in leaves after autumn collection of fruits compared to leaves collected after blooming. Fruit tree leaves are good material for isolation of chlorophylls and carotenoids for application in cosmetics, pharmaceuticals or in the food industry, e.g. production of beverages or puree.


Assuntos
Carotenoides/análise , Clorofila/análise , Cromatografia Líquida , Frutas/química , Folhas de Planta/química , Espectrometria de Massas por Ionização por Electrospray , Árvores/química , Malus/química , Rosaceae/química , Terpenos/análise
3.
Food Chem ; 348: 129055, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-33508595

RESUMO

The study evaluated the potential and antioxidant stability of nanoencapsulated carotenoid-rich extract (CE) from Cantaloupe melon (EPG). DPPH and ABTS radical scavenging assays were used to investigate the nanoencapsulation effect on antioxidant potential. CE and EPG stability were evaluated at 25 °C and 5 °C, with and without light (1600 lx) for 60 days, determining the ß-carotene concentration by UHPLC and antioxidant potential by ABTS. The antioxidant potential of carotenoids increased after nanoencapsulation (57-59%). After 60 days, there was low retention of ß-carotene (0-43.6%) in the CE, mainly at 25 °C light (0.00%) and dark (10.0%), and total loss of activity in the four conditions. EPG preserved the ß-carotene concentration in the dark at 25 °C (99.0%) and in the light (83.1%) and dark (99.0%) at 5 °C, maintaining the antioxidant potential (68.7-48.3%). Therefore, EPG enhanced and stabilized the antioxidant potential of carotenoids, beneficial to human health.


Assuntos
Antioxidantes/química , Antioxidantes/isolamento & purificação , Carotenoides/análise , Cucumis melo/química , Armazenamento de Alimentos , Gelatina/química , Nanoestruturas/química , Cápsulas , Frutas/química , Humanos
4.
J Food Sci ; 86(2): 394-403, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33462859

RESUMO

A novel deodorization method of edible oil by using ethanol steam at low-temperature was developed. We compared the chemical changes in predeodorized rapeseed oil after anhydrous ethanol steam distillation at low temperature (140 to 220 °C) (L-ESD) and conventional high-temperature (250 °C) water-steam distillation (H-WSD) in terms of odor characteristics, physicochemical properties, micronutrient contents, antioxidant performance, and fatty acid composition. Compared with H-WSD (250 °C for 60 min), L-ESD at 180 °C for 80 to 100 min resulted in lower response values of electronic nose, free fatty acid (0.03% to 0.07%), and peroxide value (0.00 to 0.67 meq/kg), but higher retention of tocopherols (554.93 to 551.59 mg/kg), total phenols (43.36 to 45.42 mgGAE/kg), total carotenoids (65.78 to 67.85 mg/kg), phytosterols (585.80 to 596.53 mg/100 g), polyunsaturated fatty acids (27.95 to 28.01%), and better antioxidant properties. In conclusion, L-ESD can mitigate the damage of oil and thus significantly improve the safety of vegetable oils with a high retention of nutrients compared with conventional H-WSD. PRACTICAL APPLICATION: The present study aimed to compare the chemical changes in predeodorized rapeseed oil after anhydrous ethanol steam distillation at low temperature (140 to 220 °C) (L-ESD) and conventional high-temperature (250 °C) water-steam distillation (H-WSD) in terms of odor characteristics, physicochemical properties, micronutrient contents, antioxidant performance, and fatty acid composition. Results indicated that this finding supplies a theoretical basis for developing a method with retaining more micronutrients and producing less harmful substances for the deodorization of rapeseed oil.


Assuntos
Etanol , Manipulação de Alimentos/métodos , Odorantes/prevenção & controle , Óleo de Brassica napus/química , Vapor , Antioxidantes/análise , Carotenoides/análise , Fenômenos Químicos , Destilação/métodos , Ácidos Graxos/análise , Micronutrientes/análise , Fitosteróis/análise , Óleo de Brassica napus/análise , Temperatura , Tocoferóis/análise
5.
Food Chem ; 346: 128888, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33385911

RESUMO

The aim of this study was to analyse contents of phytosterols, carotenoids, tocochromanols and fatty acid composition in oil from nutlets of linden. Standard determination methods for these compounds were applied using liquid and gas chromatography. In the analysed oils from small and large-leaved lime the dominant acids included linoleic (53.1 and 54.1%), oleic (18.5 and 22.2%), palmitic (15.3 and 11.8%). Linden seed oil was characterised by very high contents of phytosterols (2-2.5 g/100 g). ß-Sitosterol was the dominant phytosterol (80-83%). Additionally squalene was detected at 806 and 607 mg/100 g, respectively, for Tilia cordata and T.platyphyllos. It was found that linden seed oil contains tocopherols (93%) and tocotrienols (7%). In terms of tocochromanol contents this oil is surpassed only by wheat germ oil. In terms of the α-T equivalent these oils contain 95 (Tilia cordata) and 50.6 mg/100 g vitamin E (Tilia platyphyllos).


Assuntos
Carotenoides/análise , Tilia/química , Tocoferóis/análise , Tocotrienóis/análise , Cromatografia Gasosa , Cromatografia Líquida de Alta Pressão , Ácidos Graxos/análise , Óleos Vegetais/química , Sementes/química , Sementes/metabolismo , Tilia/metabolismo
6.
J Agric Food Chem ; 69(4): 1300-1307, 2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33494603

RESUMO

We profiled and quantified primary (amine, organic acids, tricarboxylic acid cycle intermediates, amino acids, and carbohydrates) and secondary metabolites (triterpenoids, phenolic acids, carotenoids, flavonoids, and anthocyanins) in the edible parts (leaves and fruits) of the diploid and tetraploid cultivar Morus alba L. 'Cheongil.' Through comprehensive metabolic profiling, the tetraploid mulberry cultivar was able to produce diverse metabolites supported by higher accumulation patterns of primary and secondary metabolites in their edible parts. In particular, the edible parts of the tetraploid showed higher accumulation patterns of most metabolites (amino acids, carbohydrates, carotenoids, and anthocyanins) than the diploid, which was supported by the results of principal component analyses (PCAs) showing a clear separation between the diploid and tetraploid groups. Additionally, this metabolome study comprehensively described the correlation between primary and secondary metabolites in the edible parts of diploid and tetraploid mulberry cultivars and provided information useful for plant breeding strategies to improve metabolite biosynthesis using polyploidy.


Assuntos
Frutas/química , Frutas/metabolismo , Morus/genética , Antocianinas/análise , Antocianinas/metabolismo , Carotenoides/análise , Carotenoides/metabolismo , Diploide , Frutas/genética , Metabolômica , Morus/química , Morus/metabolismo , Extratos Vegetais , Metabolismo Secundário , Tetraploidia
7.
Food Chem ; 340: 127912, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32916404

RESUMO

Palm-pressed mesocarp oil has been found to contain plenty of naturally occurring valuable phytonutrients. The application and study of the oil are limited, therefore, quality assessment of refined red palm-pressed mesocarp olein (PPMO) is deemed necessary to provide data in widening the applications as a niche products or raw material for the nutraceutical industry. Results showed that refined PPMO has comparable physicochemical properties and oxidative stability with commercial cooking oil, palm olein (PO). The food safety parameters and contaminants (PAH, 3-MCPD ester, 2-MCPD ester, glycidyl ester and trace metals) analyses proven that refined PPMO is safe to be consumed. Besides, refined PPMO contains remarkably greater concentrations of phytonutrients including carotenoids, phytosterols, squalene and vitamin E than PO, postulating its protective health benefits. The overall quality assessment of refined PPMO showed that it is suitable for human consumption and it is a good source for food applications and dietary nutritional supplements.


Assuntos
Contaminação de Alimentos/análise , Qualidade dos Alimentos , Indústria de Processamento de Alimentos , Óleo de Palmeira/química , Carotenoides/análise , Culinária , Ésteres/análise , Ácidos Graxos/análise , Glicerol/análogos & derivados , Glicerol/análise , Oxirredução , Óleo de Palmeira/análise , Fitosteróis/análise , Vitamina E/análise , alfa-Cloridrina/análise
8.
Food Chem ; 340: 127897, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32871355

RESUMO

This research study presents information for the first time on the nutritionally relevant lipophilic compounds obtained from Ecklonia radiata, a poorly studied brown kelp. The major lipophilic compounds were analyzed utilizing liquid chromatography (LC)-tandem mass spectrometry (MS/MS) and gas chromatography (GC)-mass spectrometry (MS). The LC-MS/MS results revealed the presence of eight major lipophilic compounds, including sterols, carotenoids, vitamin E, and phylloquinone (vitamin K1). Quantitative analysis showed that fucosterol was the most predominant phytosterol in the fronds and stipes of E. radiata. The carotenoids (all-E)-fucoxanthin and (all-E)-ß-carotene were present in higher yield. In terms of vitamin E, α-tocopherol was identified as the main tocol. The coenzyme, phylloquinone, important for protein synthesis, was also identified in E. radiata. GC-MS identified 13 fatty acids with palmitic (C16:0) and oleic acid (C18:1n9c) present in the highest quantities. To our knowledge, this is the first report on E. radiata, and the valuable data presented herein can be used as a baseline for developing novel nutraceuticals.


Assuntos
Kelp/química , Carotenoides/análise , Cromatografia Líquida/métodos , Ácidos Graxos/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Interações Hidrofóbicas e Hidrofílicas , Fitosteróis/análise , Esteróis/análise , Espectrometria de Massas em Tandem/métodos , Vitamina K 1/análise , Xantofilas/análise , alfa-Tocoferol/análise , beta Caroteno/análise
9.
Food Chem ; 340: 127901, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32889204

RESUMO

Cauliflowers are generally associated with healthy diets due to their positive impact on health. This research aims to evaluate the effects of cooking processes (boiling, steaming and microwaving) and different preparation times, on the content of carotenoids and provitamin A and tocopherols, in cauliflowers and to verify the effect of the cooking process on maintaining the coloring. The results revealed that the thermal processinfluencedthe antioxidant compounds releaseindependent of genotype. The highest content of zeaxanthin and lutein was found in 'Verde di Macerata' after boiling for 20 min. 'Cheddar' presented the highest content of all carotenoids and when steamed for 20 min, the highest levels ofprovitamin Awere observed.Microwaved and bolied 'Grafitti' for longer times showed the highest retention of tocopherol. The cooking did not negatively affect the visual aspect. 'Verde di Macerata' and 'Cheddar' may be good sources of carotenoids and tocopherols.


Assuntos
Brassica/química , Carotenoides/análise , Culinária/métodos , Tocoferóis/análise , Antioxidantes/análise , Antioxidantes/química , Brassica/genética , Carotenoides/química , Genótipo , Pigmentação , Fatores de Tempo , Tocoferóis/química , Vitamina E/análise , Vitamina E/química
10.
Food Chem ; 337: 127786, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32795861

RESUMO

The dehydration process is a prerequisite to preserve saffron for a long time. According to this process, saffron shows differences in the main compounds responsible for its quality (colour, taste, aroma, and flavonol content). At present, the freeze-drying method obtains dried products with the highest quality. Viruses can modify the physiology and metabolism of plants, being able to affect the activities of several enzymes. For this reason, the main compounds of saffron have been analyzed under two different dehydrating processes, freeze-drying and dark-drying, considering their infection status with the Saffron latent virus (SaLV). Results showed that the picrocrocin and safranal content enables to differ dark-dried samples from freeze-dried ones. Besides, the kaempferol-3-O-sophoroside-7-O-glucoside content allows differentiating between SaLV-infected (SaLV+) and uninfected (SaLV-) saffron samples. Moreover, our data suggest that the freeze-drying would decrease crocins content, and dark-drying can nullify the adverse effect of SaLV on crocins content.


Assuntos
Crocus/virologia , Dessecação/métodos , Compostos Fitoquímicos/análise , Viroses/epidemiologia , Carotenoides/análise , Carotenoides/metabolismo , Crocus/classificação , Crocus/metabolismo , Cicloexenos/análise , Cicloexenos/metabolismo , Glucosídeos/análise , Glucosídeos/metabolismo , Irã (Geográfico) , Quempferóis/análise , Quempferóis/metabolismo , Compostos Fitoquímicos/metabolismo , Doenças das Plantas , Prevalência , Terpenos/análise , Terpenos/metabolismo
11.
Food Chem ; 337: 127771, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32777564

RESUMO

Faveleira (Cnidoscolus quercifolius) is an emerging Brazilian plant, with seeds rich in edible oil. This study investigates physicochemical properties, chemical composition, thermal and oxidative stability, in vitro and in vivo toxicity, antioxidant, antinociceptive and anti-inflammatory activities of faveleira seed oil. It was observed that the oil has low acidity, value of peroxide, chlorophyll, carotenoids, ß-carotene and high concentrations of unsaturated fatty acids. In addition to presenting thermal and oxidative stability and high total phenolic content, with vanillin, eugenol and quercetin were predominating. The oil showed no toxicity in vitro and in vivo, and presented antioxidant, anti-inflammatory and antinociceptive activities. These findings provide relevant and appropriate conditions for processing of faveleira seed oil as functional food.


Assuntos
Euphorbiaceae/metabolismo , Óleos Vegetais/química , Analgésicos/química , Analgésicos/farmacologia , Analgésicos/uso terapêutico , Animais , Antioxidantes/química , Brasil , Carotenoides/análise , Sobrevivência Celular/efeitos dos fármacos , Clorofila/análise , Euphorbiaceae/química , Ácidos Graxos/análise , Masculino , Camundongos , Dor/induzido quimicamente , Dor/prevenção & controle , Fenóis/análise , Óleos Vegetais/farmacologia , Óleos Vegetais/uso terapêutico , Células RAW 264.7 , Sementes/química , Sementes/metabolismo
12.
Food Chem ; 345: 128772, 2021 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-33310255

RESUMO

We developed a whey protein admixture of turmeric extract by spray drying (TWPC-SD) and by foam mat drying (TWPC-FMD) and compared its bioactive compounds and nutrients contents. TWPC samples were evaluated for preference and acceptability. Vitamins and carotenoids were determined by high-performance liquid chromatography. Total phenolics, curcumin and antioxidant capacity were determined by spectrophotometry. Centesimal composition was performed according to the Association of Official Analytical Chemists. Chemical elements were determined by inductively coupled plasma optical emission spectrometry. TWPC containing 3.6 mg of curcumin showed good acceptability. Carotenoids and riboflavin were not detected in either TWPC. Vitamin C content was maintained, and antioxidant capacity was increased in both products (p < 0.05). TWPC-SD showed higher total phenolic and curcumin contents compared to TWPC-FMD (p < 0.05). Thus, the TWPC-SD is a good alternative for human consumption since it showed good sensory acceptability and its nutrients and bioactive compounds can contribute to human health.


Assuntos
Misturas Complexas/química , Curcuma/química , Nutrientes/análise , Extratos Vegetais/química , Proteínas do Soro do Leite/química , Antioxidantes/análise , Ácido Ascórbico/análise , Carotenoides/análise , Cromatografia Líquida de Alta Pressão , Dessecação , Humanos , Fenóis/análise , Vitaminas/análise
13.
Food Chem ; 340: 127951, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32896777

RESUMO

Wheat is a relevant source not only of essential macronutrients but also of many other health-promoting phytochemicals (carotenoids, anthocyanins, tocols, phenolic acids, etc.). Colored-grain wheats were used for extrusion and kernel puffing. The total content of carotenoids (sum of lutein, zeaxanthin, antheraxanthin, α- and ß-carotene, and xanthophyll esters) decreased significantly due to extrusion (to 25.7%) and puffing (to 31.6%), compared to the content in the raw material. Zeaxanthin was shown to be the most stable among all detected carotenoids (30.8 and 48.7% was preserved). The results of the performed analyses have not confirmed greater stability of xanthophyll esters against higher temperatures (decrease to 29.5 and 22.1%). Both technologies induced E-to Z-isomerization of all-E-lutein and puffing also of all-E-zeaxanthin. Higher concentrations of 13-Z- and 9-Z-zeaxanthin were identified in puffed grains (2× and 37× on average). To preserve more carotenoids, it is appropriate to look for a more suitable food processing technology.


Assuntos
Carotenoides/análise , Manipulação de Alimentos , Triticum/química , Antocianinas/análise , Grão Comestível/química
14.
J Sci Food Agric ; 101(1): 54-64, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32949019

RESUMO

BACKGROUND: Reducing the negative effects of chilling injury (CI) in tomatoes after harvest is essential to ensure good quality and to minimize losses. CI is a postharvest disorder associated with the generation of reactive oxygen species (ROS) in the fruit. Therefore, antioxidant accumulation can counteract ROS, alleviating CI symptoms. In this sense, it has been confirmed that a brief hot-water (HW) immersion promotes the synthesis of antioxidants. RESULTS: HW treatment at 52 °C for 5 min significantly reduced chilling-associated decay, from 66.7% to 17.2% in breaker turning (BT) and from 55.8% to 9.8% in mature green (MG) 'BHN-602' tomatoes stored at 5 °C for 2 weeks and from 26.7% to 6.7% in BT tomatoes stored at 5 °C for 1 week. Also, HW treatment significantly increased lycopene content by 17% in BT tomatoes stored at 5 °C for 2 weeks, as well as ascorbic acid by 11%, lipophilic phenolics by 18% and total phenolics by 6.5% in BT tomatoes stored at 12.5 °C for 1 week. Despite the increase of antioxidants, HW treatment did not enhance the sensory aromatic profile, color and antioxidant capacity. Interestingly, HW treatment reduced ripening time by 3 days in MG tomatoes stored at 5 °C for 2 weeks or at 12.5 °C for 1 week. CONCLUSION: HW treatment applied to MG or BT 'BHN-602' tomatoes can alleviate the development of some CI symptoms, particularly decay, possibly by increasing antioxidants that scavenge ROS. © 2020 Society of Chemical Industry.


Assuntos
Conservação de Alimentos/métodos , Lycopersicon esculentum/química , Água/química , Antioxidantes/análise , Antioxidantes/metabolismo , Ácido Ascórbico/análise , Ácido Ascórbico/metabolismo , Carotenoides/análise , Carotenoides/metabolismo , Temperatura Baixa , Armazenamento de Alimentos , Frutas/química , Frutas/metabolismo , Lycopersicon esculentum/metabolismo , Espécies Reativas de Oxigênio/metabolismo
15.
Food Chem ; 334: 127595, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32711277

RESUMO

The present study report on the application of an on line supercritical fluid extraction-supercritical fluid chromatography-triple quadrupole/mass spectrometry methodology to the first apocarotenoids profiling of seventeen different chilli peppers cultivars belonging to Capsicum annuum, Capsicum baccatum and Capsicum chinense species. A total of 19 free apocarotenoids and 8 apocarotenoids fatty acid esters were identified; ß-Apo-8'-carotenal and Apo-8'-zeaxanthinal were also quantified and the ß-Apo-8'-carotenal occurrence was in the percentage ranges relative to ß-carotene of 0.03-3.87%. PCA was performed as a multivariate display method on the quantified carotenoids and apocarotenoids, in order to visualize the data structure. Moreover, different ε-apoluteinals and 4-oxo-apo-ß-carotenals were detected in Capsicum species also for the first time and, to the best of authors knowledge, in any food matrix.


Assuntos
Capsicum/química , Carotenoides/análise , Cromatografia com Fluido Supercrítico/métodos , Análise de Alimentos/métodos , Análise de Alimentos/estatística & dados numéricos , Frutas/química , Espectrometria de Massas/métodos , Análise de Componente Principal , beta Caroteno/análise
16.
Food Chem ; 339: 127818, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32854038

RESUMO

Native extracts from orange peels were obtained by a conventional method using acetone and, an alternative method using ionic liquid (1-butyl-3-methylimidazolium chloride ([C4mim]Cl)). The bioaccessibilities and cellular uptakes of carotenoids, esters and chlorophylls were evaluated, since the influence of esterification on bioaccessibility and bioavailability is not well established. For this, the extracts were emulsified, submitted to in vitro simulated digestion model according to the INFOGEST protocol, followed by uptake by Caco-2 cells. Compounds were separated, identified and quantified by HPLC-PDA-MS/MS. After digestion, 22.0% and 26.2% of the total carotenoids and 45.9% and 68.7% of the chlorophylls were bioaccessible from the acetone and [C4mim]Cl extracts, respectively. The bioaccessibilities of xanthophylls and carotenes were significantly higher than those of the mono- and diesters. The uptake by Caco-2 cells varied from 130.2 to 131.9 ng/mg cell protein for total carotenoids and from 243.8 to 234.2 ng/mg cell protein for chlorophylls in the acetone and [C4mim]Cl extracts, respectively. In general, xanthophylls and esters were better absorbed than carotenes.


Assuntos
Carotenoides/farmacocinética , Fracionamento Químico/métodos , Clorofila/farmacocinética , Citrus sinensis/química , Disponibilidade Biológica , Células CACO-2 , Carotenoides/análise , Carotenoides/isolamento & purificação , Clorofila/análise , Clorofila/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Digestão , Ésteres/farmacocinética , Frutas/química , Humanos , Líquidos Iônicos/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacocinética , Espectrometria de Massas em Tandem , Xantofilas/análise , Xantofilas/isolamento & purificação , Xantofilas/farmacocinética
17.
Food Chem ; 338: 127916, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32942087

RESUMO

Spondias tuberosa is a highly perishable fruit that is difficult to transport, which limits its consumption in local areas. Thus, for economic reasons, there is a need for technologies to extend post-harvest shelf life of these fruits. The aim of this study was to evaluate the life of Spondias tuberosa fruit using edible coatings composed of Chlorella sp. and pomegranate seed oil (PSO) during cold storage. Coated and uncoated S. tuberosa fruits were stored for 12 days at 14 ± 2 °C and 85 ± 5% RH and evaluated every 3 days. Chlorella sp. + PSO coatings retarded ripening, maintained firmness, mass, and a greener color when compared to control treatment - since by default controls are not treated. Coatings in association with the best quality fruit presented 2.0% of Chlorella sp.


Assuntos
Anacardiaceae/efeitos dos fármacos , Chlorella/química , Filmes Comestíveis , Armazenamento de Alimentos/métodos , Óleos Vegetais/farmacologia , Romã (Fruta)/metabolismo , Anacardiaceae/química , Anacardiaceae/metabolismo , Carotenoides/análise , Temperatura Baixa , Cor , Frutas/química , Frutas/efeitos dos fármacos , Frutas/metabolismo , Frutas/fisiologia , Fenóis/análise , Fenóis/química , Óleos Vegetais/química , Romã (Fruta)/química , Espectrofotometria , Vitaminas/análise
18.
Food Chem ; 339: 128057, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32947106

RESUMO

The effect of light exposure on sensory quality, health-promoting phytochemical contents, and antioxidant capacity in the lateral buds of baby mustard plants was investigated at ambient storage temperature (20 °C). The results showed that light exposure (36 µmol m-2 s-1) during post-harvest storage significantly prolonged shelf life (more than 1.75-fold), delayed the weight loss and the decrease of firmness. Light treatments also enhanced chlorophyll and carotenoid contents, and retarded declines in contents of soluble sugars, ascorbic acid, flavonoids and glucosinolates, as well as antioxidant capacity. The quality of baby mustard plants receiving 24 h daily light treatment was superior to those in plants receiving 12 h treatment and constant darkness at 20 °C. These findings indicate that light exposure, especially 24 h treatment, is an effective method of prolonging shelf life and maintaining sensory and nutritional qualities in baby mustard plants stored at ambient temperature.


Assuntos
Antioxidantes/análise , Armazenamento de Alimentos/métodos , Mostardeira/química , Compostos Fitoquímicos/análise , Antioxidantes/química , Ácido Ascórbico/análise , Carotenoides/análise , Clorofila/análise , Cor , Qualidade dos Alimentos , Glucosinolatos/análise , Luz , Valor Nutritivo , Temperatura
19.
Food Chem ; 338: 128004, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32950868

RESUMO

The aim was to enhance provitamin A carotenoid (proVA CAR) concentrations and bioaccessibility in carrots by manipulating post-harvest factors. To that end, we assessed the effects of Ultraviolet-C light, pulsed light, storage temperature, and storage duration. We also measured CAR bioaccessibility by using an in vitro model. Pulsed light, but not Ultraviolet-C, treatment increased proVA CAR concentrations in the cortex tissue (p < 0.05). Longer storage times and higher temperatures also increased concentrations (p < 0.05). The maximal increase induced by pulsed light was obtained after treatment with 20 kJ/m2 and 3-days of storage at 20 °C. However, the positive effect induced by pulsed light decreased considerably over the next seven days. ProVA CAR in carrots with the highest concentrations also proved to be more bioaccessible (p < 0.05). Thus, proVA CAR concentrations in stored carrots can be increased significantly through storage times and temperatures. Pulsed light can also significantly increase proVA CAR concentrations, but only temporarily.


Assuntos
Carotenoides/análise , Daucus carota/química , Armazenamento de Alimentos/métodos , Provitaminas/análise , Disponibilidade Biológica , Carotenoides/química , Digestão , Luz , Provitaminas/química , Provitaminas/farmacocinética , Temperatura , Fatores de Tempo , Raios Ultravioleta , Vitamina A/química
20.
Nutr Metab Cardiovasc Dis ; 31(2): 592-601, 2021 02 08.
Artigo em Inglês | MEDLINE | ID: mdl-33358716

RESUMO

BACKGROUND AND AIMS: Upon consumption, carotenoids, which may attenuate cardiovascular disease (CVD) risk, diffuse from the blood and accumulate in the skin. This study aimed to assess the associations between dietary, plasma, and skin carotenoids with CVD risk indicators and to examine the mediational role of plasma carotenoids in the relationship between skin carotenoids status (SCS) and CVD risk. METHODS AND RESULTS: Dietary, plasma, and skin carotenoids were assessed in a cross-sectional study from a community in Singapore (n = 103) aged 50 to 75 y. Multiple linear regression and binary logistics regression models were used to examine the associations between the carotenoids status with classical CVD risk factors and composite CVD risk indicators. After controlling for covariates, SCS and plasma carotenoids were inversely associated with systolic blood pressure (skin: P < 0.001; plasma: P < 0.05) and diastolic blood pressure (skin: P < 0.001; plasma: P < 0.005). Additionally, each increment of 1000 in SCS was associated with an odds ratio of 0.924 (P < 0.01) for metabolic syndrome diagnosis and 0.945 (P < 0.05) for moderate to high CVD risk classification. Associations between SCS and composite CVD risk indicators were null when adjusted for the corresponding plasma carotenoids, indicating complete mediation. Dietary carotenoids, however, showed no relationship with the CVD risk indicators. CONCLUSION: Carotenoids bioavailability may be important for cardiovascular protection. SCS, driven by the corresponding plasma carotenoids, could be a potential noninvasive surrogate marker for CVD risk determination in middle-aged and older adults. CLINICAL TRIAL REGISTRATION: NCT03554954, https://clinicaltrials.gov/. TRIAL REGISTRATION DATE: 13 June 2018.


Assuntos
Doenças Cardiovasculares/metabolismo , Carotenoides/análise , Pele/química , Fatores Etários , Idoso , Biomarcadores/análise , Doenças Cardiovasculares/diagnóstico , Doenças Cardiovasculares/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Prognóstico , Medição de Risco , Singapura/epidemiologia , Fatores de Tempo
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