Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 7.578
Filtrar
1.
Gene ; 732: 144350, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31935505

RESUMO

THO complex is a multisubunit family with a function in transcription and mRNA export. In the present study, transcripts of THO complex (thoc) were identified in developing ovary of common carp and their role during ovarian development and growth has been characterized for the first time in a teleost using expression profiling and transient siRNA silencing. Thoc expression revealed a spatiotemporal pattern in the gonads with high levels at 120 days post-hatch, with moderately high levels thereafter. In situ hybridization and immunohistochemical localization revealed the presence of thoc3 in follicular layer of stage-III/IV oocytes. High levels of thoc3, thoc5, and thoc7 genes in the follicular layer suggest a possible role in ovarian growth. Reduced levels of serum estradiol-17ß and 17α, 20ß-dihydroxypregn-4-en-3-one after thoc3 transient silencing indicated differential action on steroidogenic enzyme, transcription factor, and growth factor genes. Furthermore, transient silencing of thoc3, in vivo and in vitro, downregulated ad4bp/sf1, amh, cyp19a1a, foxl2, hsd3b, hsd11b1, hsd20b, hsd17b1, rspo1, and vtg. Incidentally, gdf9 and igf1 were upregulated, while no change was seen in esr1/2, nanos, and vasa. These observations imply that thoc3 seems to regulate ovarian function including steroidogenesis, either directly or indirectly.


Assuntos
Carpas/genética , Perfilação da Expressão Gênica/veterinária , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Ovário/crescimento & desenvolvimento , Animais , Núcleo Celular/genética , Estradiol/metabolismo , Evolução Molecular , Feminino , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Folículo Ovariano , Ovário/química , RNA Interferente Pequeno/farmacologia , Diferenciação Sexual
2.
Dis Aquat Organ ; 137(3): 175-183, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31942863

RESUMO

Flavobacterial infections are among the causes of fish losses in farms with the emergence of antibiotic-resistant isolates. Silver nanoparticles (AgNPs) are known for their potent antimicrobial activity against different types of bacteria. In this study, we evaluated the antibacterial properties of AgNPs (diameter: 23 nm) against Flavobacterium johnsoniae infection in common carp Cyprinus carpio. The assays included both in vitro and in vivo antibacterial tests in addition to evaluation of cell toxicity effects on the fish cell lines. The in vitro results revealed potent inhibitory effects of AgNPs on the growth of F. johnsoniae with a minimum inhibitory concentration of 34 µg ml-1. Fish cell (epithelioma papulosum cyprini and koi carp fin) viability was 95-100% after exposure to 500 ng ml-1 (and lower concentrations) of AgNPs. In the exposure experiment, mortality rates decreased from 45% in the infected non-treated group to 30 and 15% in the intraperitoneal injection and immersion-treated groups, respectively. Neither of the treated groups showed any clinical signs or histopathological lesions. The single-dose treatment with AgNPs during early infection with F. johnsoniae aided in minimizing fish losses.


Assuntos
Carpas , Nanopartículas Metálicas , Animais , Flavobacterium , Testes de Sensibilidade Microbiana , Prata
3.
J Sci Food Agric ; 100(3): 1195-1203, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31721226

RESUMO

BACKGROUND: The present study investigated the effect of two-step heat treatments on the structure of grass carp myofibrillar proteins (MPs) and their binding ability for selected aldehydes (hexanal, heptanal, octanal and nonanal). RESULTS: Within 30 min of the first heating step at 40 °C and 5-10 min of the second heating step at 90 °C, the enhancement of the flavor-binding ability was likely explained by the increases in surface hydrophobicity and total sulfhydryl content due to the unfolding of secondary structures of MPs through exposure of hydrophobic amino acids and sulfhydryl groups. Nevertheless, lengthy heating at 90 °C accelerated the aggregation of unfolded MPs and reduced the hydrophobic bonding sites, thus weakening the hydrophobic interactions and decreasing the resultant binding ability of MPs with aldehydes. CONCLUSION: The binding ability of aldehydes to MPs was found to be strongly influenced by changes in protein structure and surface during the two-step heating process. The results provided insight into improving the flavor characteristics of freshwater fish surimi products. © 2019 Society of Chemical Industry.


Assuntos
Aldeídos/análise , Produtos Pesqueiros/análise , Proteínas de Peixes/química , Miofibrilas/química , Animais , Carpas , Aromatizantes/química , Temperatura Alta , Interações Hidrofóbicas e Hidrofílicas , Estrutura Secundária de Proteína
4.
Food Chem ; 303: 125367, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31442901

RESUMO

Fish muscle firmness is an important quality trait for consumer acceptance. Phosphorylation is known to change chemical and physical properties of proteins and is thus expected to affect muscle firmness, but only few such phosphoproteins have been identified. To explore phosphoproteins that affect fish muscle firmness, firm muscle (crisp grass carp) and soft muscle (ordinary grass carp) were analyzed by quantitative phosphoproteomics. We identified 27 up-regulated and 22 down-regulated phosphopeptides in crisp grass carp (ratio ≥1.5 or ≤0.667, and P-value < 0.05) and their potential upstream kinases. Protein-protein interaction analysis clustered these phosphoproteins into four groups, many of which have been suggested to impact muscle firmness and its postmortem changes: muscle fiber, connective tissue, carbohydrate metabolism and signal regulation. These results provide novel insights into the role of protein phosphorylation in fish muscle firmness and will contribute to the quality improvement of fish products.


Assuntos
Carpas/metabolismo , Proteínas de Peixes/metabolismo , Músculos/metabolismo , Fosfoproteínas/metabolismo , Proteômica , Animais , Fenômenos Biomecânicos , Alimentos Marinhos
5.
Food Chem ; 308: 125576, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31648092

RESUMO

This study investigated the effects of cold storage at different temperatures (4, -0.5, -3, and -20 °C) on protein degradation and its relationship to structural changes of black carp muscle. At -0.5 and 4 °C, major structural changes occurred, including the formation of gaps between myofibers and myofibrils, breakage of myofibrils and myofibers, and degradation of sarcoplasmic reticulum. Gel-based proteomic analysis showed that these structural changes were accompanied by degradation of a series of myofibrillar proteins, including titin, nebulin, troponin, myosin, myomesin, myosin-binding protein, and α-actinin. Loss of extractable gelatinolytic and caseinolytic protease activities was also observed. At -3 and -20 °C, formation of ice crystals was the most noticeable change. The major proteins were degraded at different locations in the black carp muscle, and gelatinolytic and caseinolytic proteases appear to contribute to the degradation of those proteins.


Assuntos
Carpas/metabolismo , Actinina/metabolismo , Animais , Proteínas de Transporte , Temperatura Baixa , Conectina/metabolismo , Cyprinidae/metabolismo , Proteínas Musculares/metabolismo , Miofibrilas/metabolismo , Miosinas/metabolismo , Proteólise , Proteômica
6.
Ecotoxicol Environ Saf ; 188: 109912, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31706240

RESUMO

Synthetic progestins are emerging contaminants of the aquatic environment with endocrine disrupting potential. The main aim of the present study was to investigate the effects of the synthetic progestins gestodene, and drospirenone on sex differentiation in common carp (Cyprinus carpio) by histological analysis. To gain insights into the mechanisms behind the observations from the in vivo experiment on sex differentiation, we analyzed expression of genes involved in hypothalamus-pituitary-gonad (HPG) and hypothalamus-pituitary-thyroid (HPT) axes, histology of hepatopancreas, and in vitro bioassays. Carp were continuously exposed to concentrations of 2 ng/L of single progestins (gestodene or drospirenone) or to their mixture at concentration 2 ng/L of each. The exposure started 24 h after fertilization of eggs and concluded 160 days post-hatching. Our results showed that exposure of common carp to a binary mixture of drospirenone and gestodene caused increased incidence of intersex (32%) when compared to clean water and solvent control groups (both 3%). Intersex most probably was induced by a combination of multiple modes of action of the studied substances, namely anti-gonadotropic activity, interference with androgen receptor, and potentially also with HPT axis or estrogen receptor.


Assuntos
Androstenos/toxicidade , Carpas/crescimento & desenvolvimento , Disruptores Endócrinos/toxicidade , Norpregnenos/toxicidade , Diferenciação Sexual/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Relação Dose-Resposta a Droga , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Gônadas/efeitos dos fármacos , Hepatopâncreas/efeitos dos fármacos , Hipotálamo/efeitos dos fármacos , Hipófise/efeitos dos fármacos , Diferenciação Sexual/genética
7.
Chemosphere ; 240: 124969, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31726589

RESUMO

The objective of this work was to evaluate whether tetracycline (TC) in environmentally relevant concentrations was able to induce alterations to embryonic development and teratogenic effects in oocytes and embryos of Cyprinus carpio. For this purpose, an embryolethality study was conducted and the lethal concentration 50 (LC50) and effective concentration 50 of malformations (EC50) were calculated, and with these data the teratogenic index (TI) was determined. The main alterations to embryonic development and the teratogenic effects produced by TC on embryos of C. carpio were determined using the Kimmel and Hersem scale adapted for Cyprinus carpio. LC50 and EC50 were respectively 500.08 and 145.3 µg L-1.TC was shown to be teratogenic with teratogenic index of 3.44, and the main malformations identified in concentrations of 90-900 µg L-1 were malformation in tail, modified chorda structure, pericardical edema, scoliosis and malformations of the heart. A significant decrease in concentration-dependent in Kimmel and Hersem score was observed. The results allow us to conclude that TC at environmentally relevant concentrations is capable of inducing embryotoxic and teratogenic effects, generating risk in the integrity of the common carp C. Carpio.


Assuntos
Carpas/embriologia , Desenvolvimento Embrionário/efeitos dos fármacos , Poluentes Ambientais/toxicidade , Teratogênios/farmacologia , Tetraciclina/toxicidade , Animais , Dose Letal Mediana , Teratogênese
8.
Environ Monit Assess ; 191(11): 700, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31667668

RESUMO

The distribution of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) in the muscle, liver, spleen and kidney tissue of two fish species was studied using an optimised diatomaceous earth assisted modified QuEChERS extraction method. Five-year-old free-ranging male African sharptooth catfish (Clarias gariepinus) and 5-year-old male common carp (Cyprinus carpio) sampled from the Hartbeespoort Dam in South Africa were used for method development. Acetonitrile extraction produced more precise recoveries than hexane extraction. Fluorene and naphthalene were the most abundant PAHs detected in the majority of fish tissues analysed. PAH bioaccumulation in 5-year-old carp and 5-year-old catfish was in the order muscle > kidney > liver > spleen and liver > muscle > kidney > spleen, respectively. PCBs were mostly detected in carp spleen and kidney. Two-year-old carp were analysed to determine PCB and PAH bioaccumulation trends. The differences in ∑16PAH concentrations between the four organs tested were all statistically insignificant for the 3 fish tested (p > 0.05). All other organs with the exception of 5-year-old carp spleen and 5-year-old carp kidney recorded total 31 PCB concentrations (∑31PCB) < 25 ng g-1. Only 5-year-old carp spleen (∑31PCB of 592 ng g-1) and 5-year-old carp kidney (∑31PCB of 561 ng g-1) had significant differences (p < 0.05) from the spleen and kidney in 5-year-old catfish and 2-year-old carp. Whilst the carp and catfish sampled can be considered low PCB risk foods, 5-year-old carp muscle can be considered to be a high PAH risk food, with a benzo(a)pyrene concentration of 7 µg g-1, based on the EU Commission Regulation 2005/208/EC pertaining to the maximum permissible benzo(a)pyrene level in fresh fish muscle.


Assuntos
Carpas/metabolismo , Peixes-Gato/metabolismo , Monitoramento Ambiental , Bifenilos Policlorados/metabolismo , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Poluentes Químicos da Água/metabolismo , Animais , Bioacumulação , Peixes-Gato/fisiologia , Fígado/metabolismo , Masculino , Músculos/química , Bifenilos Policlorados/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , África do Sul
9.
Zootaxa ; 4657(1): zootaxa.4657.1.4, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31716798

RESUMO

During an ongoing investigation into the myxosporean diversity of common carp in Chongqing, China, Myxobolus parakoi sp. nov. was found to infect the gill lamellae of Cyprinus carpio (Linnaeus, 1758). Both morphological and SSU rDNA data revealed that M. parakoi sp. nov. was distinct from other myxosporeans. Mature myxospores of M. parakoi sp. nov. were pyriform in the frontal view. The spores were 15.98 ± 0.78 (14.59-17.72) µm in length and 7.84 ± 0.78 (6.66-9.75) µm in width. The two polar capsules were pyriform and equal in size, exhibiting 8.72 ± 0.50 (7.76-9.92) µm in length and 3.03 ± 0.23 (2.63-3.56) µm in width. Polar filaments within the polar capsules were coiled with 11 or 12 turns. Phylogenetic analysis revealed that M. parakoi sp. nov. and Myxobolus koi (FJ710800) were clustered together, forming a sister subclade with Myxobolus tanakai. This study also implied that the morphology of the myxospores was generally correlated with their phylogenetic relationship, while such correlation was not strong or consistent.


Assuntos
Carpas , Doenças dos Peixes , Myxobolus , Myxozoa , Doenças Parasitárias em Animais , Animais , China , Brânquias , Filogenia
10.
J Water Health ; 17(5): 762-776, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31638027

RESUMO

Disinfection is intended to improve drinking water quality and human health. Although disinfectants may transform organic matter and form disinfection by-products (DBPs), many are branded as cyto- and genotoxic. Traditionally, research focuses on the effects of DBPs on human health, but cytogenic impacts on aquatic organisms still remain ill defined. The current study examines the potential toxic effect of chloroform and iodoform (DBPs) on Cyprinus carpio, selected as a model organism. Fish specimens were exposed to various concentrations of DBPs primarily based on LD50 values, where acute toxicity was monitored for 96 h. Headspace SPME extraction through gas chromatography was employed to assess the effects of spiked DBPs doses in fish blood. Cytotoxicity was monitored using Comet assay. Tail length, tail DNA, and olive tail moment values were quantified to be significant (P < 0.05) as compared to control. A statistically significant (P < 0.05) decrease in all blood parameters (hematology) was observed. Changes in biochemical indices (glucose, total protein, and alanine aminotransferase (ALT)) were also significant. ALT secretion was significantly increased (93 ± 0.05 and 82.8 ± 0.1 U/L) at higher concentration compared to control (56 ± 0.1 U/L), suggesting liver damage. Results demonstrated that iodoform was statistically more damaging as compared to chloroform.


Assuntos
Carpas/fisiologia , Desinfetantes/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Dano ao DNA , Desinfecção , Hematologia , Humanos
11.
Environ Monit Assess ; 191(11): 672, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31650301

RESUMO

Environmental DNA (eDNA) has been used to detect the presence of various species in aquatic ecosystems, but its degradation by several environmental factors can influence the correct identification of aquatic organisms. The present study examined the effects of a pesticide, diazinon, on breakage of Cyprinus carpio eDNA. The specimens were exposed to 0 (control), 0.06, 0.1, and 1 ppm of diazinon for 9 days. Water samples were collected at three time points (3, 6, and 9 days postexposure, dpe), and eDNA was extracted. The cytochrome oxidase I (COI) gene was successfully amplified by PCR, and a fuzzy inference system was used to convert DNA smears and breakage to numerical values. eDNA breakage percentage increased with diazinon concentration at all sampling times. At 3 dpe, the maximum eDNA breakage percentage occurred at 0.06 and 0.1 ppm of diazinon; whereas at 6 and 9 dpe, the maximum breakage was found at 1 ppm of diazinon, while exposure time had no significant effect. To the best of our knowledge, this is the first study to demonstrate that eDNA integrity can be compromised by a diazinon in surface waters. Hence, it is recommended that future eDNA studies take into account pesticide pollution when detecting aquatic species.


Assuntos
Carpas/genética , Quebras de DNA/efeitos dos fármacos , Diazinon/toxicidade , Monitoramento Ambiental/métodos , Inseticidas/toxicidade , Animais , Ecossistema , Complexo IV da Cadeia de Transporte de Elétrons/genética , Reação em Cadeia da Polimerase
12.
Environ Pollut ; 255(Pt 2): 113327, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31600703

RESUMO

Fish-associated antibiotic resistance genes (ARGs) have attracted increasing attention due to their potential risks to human beings via the food chain. However, data are scarce regarding the antibiotic resistance in fish themselves. Herein, the antibiotic resistance genes (ARGs) were assessed in the gut of four major Chinese freshwater carp (i.e., silver carp, grass carp, bighead carp, and crucian carp) from food retail markets. Results show that the abundances of target ARGs (e.g., tetA, tetO, tetQ, tetW, sulI, sulII, and blaTEM-1) and class 1 integrase (intI1) were in the range 9.4 × 10-6 - 1.6 × 10-1 and 6.7 × 10-5 - 5.2 × 10-2 gene copies per 16S rRNA gene, respectively. The sulI, sulII, and tetQ strongly correlated with silver and mercury resistance genes (e.g., silE and merR). The microbial taxa of fish gut could be partly separated among retail markets based on the PCA analysis. About 15.0% of the OTUs in fish gut were shared and 74.5% of the shared OTUs were identified as Acidobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, and Proteobacteria. These phyla may constitute the core microbiota in the guts of the four Chinese freshwater carp. The possible ARG hosts were revealed based on the network analysis, and the presence of pathogen-associated resistant genera in fish gut highlights the need to fully understand their potential human health risks.


Assuntos
Carpas/microbiologia , Farmacorresistência Bacteriana/genética , Microbioma Gastrointestinal/efeitos dos fármacos , Alimentos Marinhos/microbiologia , Acidobacteria/efeitos dos fármacos , Acidobacteria/genética , Acidobacteria/isolamento & purificação , Animais , Antibacterianos/farmacologia , Bacteroidetes/efeitos dos fármacos , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Chloroflexi/efeitos dos fármacos , Chloroflexi/genética , Chloroflexi/isolamento & purificação , Cianobactérias/efeitos dos fármacos , Cianobactérias/genética , Cianobactérias/isolamento & purificação , Firmicutes/efeitos dos fármacos , Firmicutes/genética , Firmicutes/isolamento & purificação , Água Doce/microbiologia , Microbioma Gastrointestinal/genética , Genes Bacterianos/genética , Metais Pesados/toxicidade , Proteobactérias/efeitos dos fármacos , Proteobactérias/genética , Proteobactérias/isolamento & purificação , RNA Ribossômico 16S/genética
13.
Dis Aquat Organ ; 136(2): 193-197, 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31621652

RESUMO

Carp edema virus disease (CEVD), also known as koi sleepy disease (KSD), is an emerging viral disease caused by the carp edema virus (CEV). It is characterized by lethargic behavior, gill necrosis, and generalized edema, leading to significant morbidity and mortality in common carp and koi Cyprinus carpio. Accurate diagnosis of CEVD relies on amplification of a P4a protein-encoding DNA segment from the CEV genome. A phylogenetic analysis of amplified fragments revealed 3 distinct CEV genogroups: I, IIa, and IIb. We explored the phylogenetic relationship between Austrian CEV isolates with existing CEV genogroups. The phylogenetic analysis (n = 18) established the presence of the 3 extant CEV genogroups as well as 2 new CEV genogroups (IIIa and IIIb) classified to identify the Austrian isolates that were distinct from the existing CEV genogroups. It is evident that CEV infection cases are growing in number each year, which may be due to development of sensitive diagnostic assays, while information regarding the virus is scarce. National and international efforts are required to study the epidemiology of the CEV in major carp-producing countries.


Assuntos
Carpas , Doenças dos Peixes , Infecções por Poxviridae , Poxviridae , Animais , Áustria , Edema , Genótipo , Filogenia , Infecções por Poxviridae/veterinária
14.
Environ Sci Pollut Res Int ; 26(32): 33311-33320, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31520375

RESUMO

In this study, the levels of 11 heavy metal(loid)s (HMs) were determined in ten different fish species (wild, farmed, freshwater, and marine) frequently consumed in Turkey and compared with the maximum permissible limits (MPLs) established by international standards. Also, human health risks for the consumers were assessed. Among 11 HMs, only Al and Cr levels did not show significant differences among fish species. The highest concentrations of As were found in red mullet, which is a demersal fish. The average concentrations of Cd, Cr, Cu, and Pb in all fish species were below the MPLs, whereas those of Zn in European anchovy and Mediterranean horse mackerel, and that of inorganic As in red mullet exceeded the MPLs. The highest and lowest toxic metal contents (Al, As, Cd, and Pb) were recorded in red mullet and farmed rainbow trout, respectively. The estimated daily intakes of HMs in each fish species were very lower than their corresponding tolerable daily intakes, suggesting the daily intake of HMs through fish consumption would not pose health risks for the consumers. The target hazard quotient for individual HMs and hazard index for combined HMs in fish species were below 1, which indicated that non-carcinogenic health effects are not expected. The carcinogenic risk results revealed that there was a carcinogenic risk from exposure to inorganic As via the consumption of red mullet. Among all investigated fish species, rainbow trout and common carp were safer in terms of both non-carcinogenic and carcinogenic risks from exposure to HMs.


Assuntos
Arsênico/análise , Exposição Dietética/estatística & dados numéricos , Metais Pesados/análise , Alimentos Marinhos/estatística & dados numéricos , Poluentes Químicos da Água/análise , Animais , Carpas , Monitoramento Ambiental , Contaminação de Alimentos/análise , Água Doce , Humanos , Oncorhynchus mykiss , Medição de Risco , Alimentos Marinhos/análise , Turquia
15.
Environ Pollut ; 255(Pt 1): 113213, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31541825

RESUMO

However the physiological stress in aquatic organisms associated with hydropower plants (HPP) ecosystems has been previously investigated, no studies have so far assessed it on biochemical level. Therefore this study evaluated an oxidative stress and toxicity in the gibel carp Carassius auratus gibelio associated with a small-scale HPP in the West Ukraine. A battery of liver, brain and blood markers was evaluated individuals inhabiting upstream and downstream of the dam of the small-scale Kasperivtci HPP (KHPP; an installed capacity of 7.5 MW), and from a reference site. Number of alterations were noted in fish from the KHPP impoundment facility including signs of oxidative stress (a decrease in superoxide dismutase (SOD) activity and an increase in protein carbonyls) and cytotoxicity (an increase in micronucleated erythrocytes and caspase-3 activity). No changes in DNA fragmentation in hepatocytes or brain cholinesterase activity were detected. As demonstrated by the integral stress index, fish associated with downstream of the dam revealed the greatest alterations reflected by the combined oppression of antioxidant system (SOD, catalase) and pro-oxidants (thiobarbituric acid reactive substances and oxyradicals), low concentration of metallothioneins, but high cathepsin D activity (as markers of lysosomal dysfunction and autophagy) and increased vitellogenin concentration in males (indicating an endocrine disruption). The study highlights that fish inhabiting ecosystems associated with HPP, particularly downstream of the dam, may face additional stresses with long-term effects yet to be evaluated.


Assuntos
Biomarcadores/metabolismo , Monitoramento Ambiental , Carpa Dourada/fisiologia , Centrais Elétricas , Estresse Fisiológico/fisiologia , Animais , Antioxidantes/metabolismo , Carpas/metabolismo , Catalase/metabolismo , Ecossistema , Fígado/metabolismo , Masculino , Estresse Oxidativo
16.
Chemosphere ; 236: 124869, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31549675

RESUMO

Chronic arsenicosis has threatened the survival of aquatic animals with molecular mechanisms yet clear. In the present study, liver damage was evident by fluctuated activities of transaminases and declined ATPases in common carp under arsenic (As) exposure for 30 days. Mechanically, As significantly decreased cytochrome P-1A (CYP1A) activity and increased reactive oxygen species (ROS) content, which corroborated mitochondrial dysfunction in the hepatocytes. This hypothesis was further suggested by Caspase-3-executed apoptosis by death receptor pathway (Fas, TNF-α and Caspase-8) and mitochondrial pathway (Bax, Bcl-2 and Caspase-9). The above results indicated that As-elicited oxidative damage lead to apoptotic hepatic injury in carp. On the contrary, zinc (Zn) exerted an ROS scavenger and an antidote to As in the present model evidenced by alleviated liver injury and restored liver function index. Moreover, Zn and As co-administration displayed partially recovered CYPs enzyme system and quenched apoptotic positive cells compared As treated alone. These outcomes could be applied to develop counter practices based on Zn preparations to decrease the biotoxicity of As.


Assuntos
Apoptose/efeitos dos fármacos , Arsenitos/toxicidade , Carpas/metabolismo , Sistema Enzimático do Citocromo P-450/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Poluentes Químicos da Água/toxicidade , Zinco/farmacologia , Animais , Caspase 3 , Caspase 9 , Hepatócitos/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Mitocôndrias/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2 , Poluentes Químicos da Água/metabolismo , Zinco/metabolismo
17.
Nat Ecol Evol ; 3(10): 1415-1418, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31527727

RESUMO

Despite the growing importance of farmed fish for contemporary economies, the origins of aquaculture are poorly known. Although it is widely assumed that fish domestication began much later than the domestication of land animals, the evidence is largely negative. Here, we use age-mortality and species-selection profiles of fish bones from prehistoric East Asia to show that managed aquaculture of common carp (Cyprinus carpio) was present at the Early Neolithic Jiahu site, Henan Province, China, by around 6000 BC.


Assuntos
Carpas , Animais , Aquicultura , China , Extremo Oriente , Alimentos Marinhos
18.
Environ Monit Assess ; 191(10): 638, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31529160

RESUMO

Heavy metal pollution in aquatic bodies is a matter of serious concern for aquatic fauna and human health. This study evaluated the effect of heavy metals on structural aberrations in fish scales of freshwater Indian major carp Labeo rohita. Scales from two size groups-lesser than and equal to 40 cm and greater than 40 cm-collected from three markets of Ludhiana district for a period of 6 months were assessed. The values of Pb, Zn and Cr in fish scales exceeded the permissible limits. Structural alterations such as distorted focus, damaged and eroded circuli, distorted pattern of circuli, damaged margins of scales, dispersed chromatophores, disorganised radii and damaged tubercles were observed on the dorsal surface of scales using light microscopy. It is postulated that heavy metals would be responsible for structural anomalies on scales as their accumulation was higher in larger fishes which have been exposed to metals for longer duration. These observations clearly suggest that fish scales are effective non-invasive indicator of water quality where fish have been reared and cultured.


Assuntos
Carpas , Monitoramento Ambiental/métodos , Metais Pesados/análise , Poluentes Químicos da Água/análise , Animais , Água Doce , Humanos , Microscopia , Poluição da Água/análise , Qualidade da Água
19.
Environ Sci Pollut Res Int ; 26(32): 33466-33477, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31522399

RESUMO

The freshwater polyculture pond culturing occupied an important position in the aquaculture industry. Accumulation of trace metals was investigated in water, sediments, and fish (Carassius auratus, Cyprinus carpio, Ctenopharyngodon idellus) from typical polyculture ponds in Chengdu, China. The results showed most of the pond water in Chengdu were safe for fish cultivation. The Cd and Cr concentrations in sediment samples from sites S3, S4, and S9 which were near the industrial park and road with a high traffic volume were higher than those of the other sites. Cu, Cr, Fe, Zn, Mn, Ni, and Pb in sediments were unpolluted, while Cd was unpolluted to moderately polluted due to anthropogenic activities. Cu, Cd, and Pb in fish pond sediment of Chengdu had higher potential mobility under normal environmental circumstances. The trace metal concentrations in liver of three fish species were all higher than those in muscle tissues. The order of bioaccumulation factor (BAF) values for trace metals was Cr > Cu > Pb > Zn > Cd > Ni > 20. The concentrations of Cu, Cd, Pb, Zn, and Cr in the muscle of three fish species were all below the local and international maximum permissible levels. The target hazard quotient (THQ) and hazard index (HI) of trace metals in aquaculture fish ponds in Chengdu were lower than 1, which indicated that the consumption of grass, crucian, and common carp cultivated in the aquaculture ponds of Chengdu pose no health risk to the residents.


Assuntos
Aquicultura , Monitoramento Ambiental , Poluentes Químicos da Água/metabolismo , Animais , Bioacumulação , Carpas/metabolismo , China , Água Doce , Sedimentos Geológicos , Carpa Dourada , Metais Pesados/análise , Metais Pesados/metabolismo , Músculos/química , Tanques , Medição de Risco , Alimentos Marinhos , Poluentes Químicos da Água/análise
20.
Aquat Toxicol ; 215: 105286, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31479757

RESUMO

Chlorpyrifos (CPF) is an environmental pollutant with increasing importance due to its high toxicity to fish and aquatic animals. In the present study, we divided 120 common carp (Cyprinus carpio L.) into two groups including control group and CPF group, CPF group was exposed to 14.5 µg/L CPF for 30 d. 17 miRNAs were differentially expressed in CPF group head kidney tissues according to the results of miRNAome analysis. In addition, histopathological examination and electron microscopy proved that CPF exposure could lead to damage of head kidney and obvious apoptosis characteristics. The possible target genes of miRNA were predicted using online target gene prediction websites, miRNAome sequencing, GO and KEGG enrichment. miRNAome results showed that expression of miR-731 and miR-2188-3p in CPF group was 0.48 time and 0.45 time as control group, respectively. qRT-PCR results proved the reality of miRNAome. During CPF exposure, mRNA expression of TLR pathway genes and its downstream genes involved in autophagy and apoptosis pathway including TLR1, TLR2, TLR7, TLR9, MyD88, IRAK1, IRAK4, IRF7, PI3K, AKT, mTOR, Caspase3, Caspase8 and Bax were differentially increased under CPF exposure, along with ATG13 and Bcl2 decreased at the same time. Western blot results indicated that apoptosis related protein Caspase3 and Caspase8 were differentially up-regulated in the CPF group. In summary, CPF exposure could induce apoptosis while inhibited autophagy in head kidney of common carp via the regulation of miR-2188-3p and miR-731 by targeting TLR pathway. These results provide new insights for unveiling the biological effects of CPF and miRNAs in common carp.


Assuntos
Apoptose/genética , Carpas/genética , Clorpirifos/toxicidade , Rim Cefálico/lesões , MicroRNAs/metabolismo , Transdução de Sinais , Receptores Toll-Like/metabolismo , Animais , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Autofagia/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Ontologia Genética , Rim Cefálico/efeitos dos fármacos , Rim Cefálico/metabolismo , Rim Cefálico/ultraestrutura , MicroRNAs/química , MicroRNAs/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reprodutibilidade dos Testes , Transdução de Sinais/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA