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1.
Mutat Res ; 850-851: 503152, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32247560

RESUMO

The aim of the present study was to assess the oxidative stress level and chromosomal damage induced by occupational exposure to low dose ionizing radiation (LDIR). Two hundred and eighteen hospital workers occupationally exposed to LDIR were included in this study, along with 118 healthy age- and gender-comparable controls. Occupational dosimetry records were collected over the last year and revealed that the accumulated annual dose for each hospital worker was below the permissible limit of the International Commission on Radiological Protection (ICRP). The individuals' oxidative and antioxidative status were determined by measuring the activities of copper zinc-superoxide dismutase (CuZn-SOD), glutathione peroxidase (GSH-Px), catalase (CAT) enzymes, and the levels of malondialdehyde (MDA) in erythrocytes. The effect of radiation on chromosomal integrity was measured by the frequency of micronuclei (MN) formation using the cytokinesis block technique. Our results showed that the activities of CuZn-SOD and CAT enzymes and MDA levels observed in the hospital workers were higher than those in the controls (p < 0.05). We did not find significant difference in GSH-Px enzyme activity between the two groups (p = 0.247). A higher frequency of MN was found in exposed groups than in the controls [3(1-5) ‰ versus 2(0.75-4) ‰; p<0.001]. The difference was significant for males (p = 0.012), but not females (p = 0.14). Multiple linear regression analysis showed differences in the oxidant activities and MN frequency between hospital workers and controls adjusted for age, gender, smoking status and drinking status. Correlation analysis indicated that the frequency of MN was positively associated with MDA levels (p < 0.05). Altogether, these results support the detrimental effects of chronic low dose radiation in humans, which involves the induction of oxidative stress and chromosomal damage.


Assuntos
Antioxidantes/metabolismo , Aberrações Cromossômicas/efeitos da radiação , Exposição Ocupacional/efeitos adversos , Estresse Oxidativo/efeitos da radiação , Adulto , Antioxidantes/efeitos da radiação , Catalase/sangue , Feminino , Glutationa Peroxidase/sangue , Hospitais , Humanos , Masculino , Malondialdeído/sangue , Registros Médicos , Pessoa de Meia-Idade , Doses de Radiação , Radiação Ionizante , Radiometria , Superóxido Dismutase-1/sangue
2.
Klin Lab Diagn ; 65(3): 174-178, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32163692

RESUMO

The pathology of the hip joints in children is the object of increased attention of specialists both in our country and abroad. Despite the wide coverage of this problem, most studies are devoted to analysis of the advantages of various surgical intervention technologies. To determine the significance of differences in hemogram indices in children with different nosological forms of hip joint pathology and indicators of healthy children. Hemogram and leukoformula parameters were determined in 10 healthy children and 47 children with hip joint pathology. The significance of differences in indicators compared with the norm and between different nosological groups of patients was evaluated. The dynamics of hemogram changes was evaluated at a long time after surgery. The largest deviations from normal hemogram values were found in a group of children with hip dysplasia complicated by aseptic necrosis of the femoral head. Two months after surgery, the main hemogram parameters in all groups of patients are normalized. The reactions of lipid peroxidation are changing, as evidenced by a decrease in the number of peroxidation products, primarily diene conjugates. Along with this, the activity of individual antioxidant enzymes changes - the activity of catalase decreases and the activity of superoxide dismutase increases. The most extreme values were noted in the group of children with epiphyseal dysplasia. In children with hip dysplasia complicated by aseptic necrosis of the femoral head, prior to surgical treatment, a statistically significant increase in the number of leukocytes and platelets in the blood serum is observed. In patients with epiphyseal dysplasia, osteochondropathy, both in the early and late stages, changes in the hemogram indices relate to the indicators of red blood cells and hemoglobin. In children with aseptic necrosis of the femoral head, hemogram values did not have statistically significant differences from the norm. In all groups of patients, destabilization of the peroxidation and antioxidant defense systems was noted.


Assuntos
Luxação Congênita de Quadril/cirurgia , Articulação do Quadril/cirurgia , Técnica de Ilizarov , Catalase/sangue , Criança , Cabeça do Fêmur/patologia , Luxação Congênita de Quadril/sangue , Articulação do Quadril/patologia , Humanos , Peroxidação de Lipídeos , Superóxido Dismutase/sangue
3.
Anticancer Res ; 40(1): 253-259, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31892574

RESUMO

BACKGROUND/AIM: The aim of the study was to compare the MDA (malonidialdehyde) plasma concentrations versus CAT (catalase)/NT (nitrotyrosine) plasma concentrations, patient satisfaction and pain score at rest/pressure to the wound area in laparotomy patients with rectus sheath block (RSB) analgesia. PATIENTS AND METHODS: Initially, 56 patients were randomized to four groups; control group (n=12), single-dose (n=16), repeated-dose (n=12) and continuous infusion (n=16) RSB analgesia groups. The plasma concentrations of CAT, NT and MDA markers were measured just before, immediately after and 24 h after operation. RESULTS: The RSB analgesia enhanced significantly patient satisfaction (p=0.001). The plasma MDA decreased immediately after operation (POP1) and the postoperative decrease between the preoperative and the POP1 values in the MDA marker were statistically significant (p<0.001). In linear mixed model, the time effect in both the single group and in the benign group in plasma NT biomarker was statistically significant (p=0.001, p=0.013, respectively). The median plasma MDA concentrations (ng/ml) following surgery were significantly lower in patients with cancer versus patients with benign disease (589 vs. 852, p=0.021). Jitterplots of the individual plasma NT versus plasma MDA showed that there was significant correlation in benign and cancer patients (r=0.347, p<0.001). CONCLUSION: Plasma MDA decreased significantly after operation in all patients and cancer patients had significantly lower MDA concentrations following surgery than patients with benign disease.


Assuntos
Analgesia , Laparotomia , Malondialdeído/sangue , Neoplasias/sangue , Bloqueio Nervoso , Catalase/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Tirosina/análogos & derivados , Tirosina/sangue
4.
Mater Sci Eng C Mater Biol Appl ; 108: 110456, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31924021

RESUMO

Under different pathological conditions, high levels of reactive oxygen species (ROS) cause substantial damage to multiple organs. To counter these ROS levels in multiple organs, we have engineered highly potent novel terpolymers. We found that combination of FDA-approved polyethylene glycol, fumaric acid moieties and electroactive tetra(aniline) by varying the content of tetra(aniline) results into a novel drug composition with biologically active and tunable intrinsic antioxidant properties. To test the intrinsic antioxidative properties of these novel terpolymers, we used alloxan to induce diabetes in rats where ROS generation is known to be higher. The systemic administration of terpolymers to the diabetic rats showed strong electroactive antioxidant behavior which not only normalized ROS levels, but also improved the levels of enzymatic antioxidants including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH). As a proof-of-principle, we here show TANI based novel drug composition of terpolymers with tunable intrinsic antioxidant properties in multiple organs.


Assuntos
Compostos de Anilina , Antioxidantes , Diabetes Mellitus Experimental/tratamento farmacológico , Compostos de Anilina/síntese química , Compostos de Anilina/química , Compostos de Anilina/farmacocinética , Compostos de Anilina/farmacologia , Animais , Antioxidantes/síntese química , Antioxidantes/química , Antioxidantes/farmacocinética , Antioxidantes/farmacologia , Catalase/sangue , Linhagem Celular , Diabetes Mellitus Experimental/sangue , Glutationa/sangue , Humanos , Masculino , Ratos , Espécies Reativas de Oxigênio , Superóxido Dismutase/sangue
5.
Lasers Med Sci ; 35(1): 131-138, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31183584

RESUMO

The main purpose of this study is to evaluate the effect of intravenous irradiation of different low-level laser wavelengths on the activity of lecithin-cholesterol acyltransferase (LCAT), paraoxonase (PON1), catalase, and ferric reducing ability of plasma (FRAP) in diabetic rats. First, diabetes was induced in rats using streptozotocin (STZ). Enzymes' activity was measured in the blood samples and compared before and after intravenous laser blood irradiation. We used four continuous-wave lasers-IR (λ = 808 nm), Red (λ = 638 nm), Green (λ = 532 nm), and Blue (λ = 450 nm)-to compare the wavelength's effect on different enzymes' activity. Laser power was fixed at 0.01 mW and laser energy was changed by 2-, 4-, 6-, and 8-min time of radiations.The enzymes' activity of blood samples was measured 2, 6, and 24 h after radiation. The results show an increase in the activity of different enzymes when compare with diabetic non-radiated samples. More importantly, with a constant laser energy, the enzymes' activity increased with decreasing laser wavelength. It is important to note that with a constant laser energy, as the wavelength decreases, the photon energy increases and the number of photons decrease, while the enzyme's activity elevation increases. As a result, we can conclude that in intravenous low-level laser therapy, photon energy is more important than the number of photons even if their product, energy, is kept constant.


Assuntos
Antioxidantes/metabolismo , Arildialquilfosfatase/sangue , Catalase/sangue , Diabetes Mellitus Experimental/sangue , Lasers , Fosfatidilcolina-Esterol O-Aciltransferase/sangue , Animais , Diabetes Mellitus Experimental/enzimologia , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/terapia , Ferro/metabolismo , Masculino , Ratos
6.
Life Sci ; 248: 116481, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31102744

RESUMO

AIMS: Hypobaric hypoxia (HH), linked to oxidative stress, impairs cardiac function. We synthesized a novel nitronyl nitroxide radical, an HPN derivative (HEPN) and investigated the protective effects of HEPN and HPN against HH-induced heart injury in mice and the underlying mechanisms of action. MAIN METHODS: Mice were administered with HPN (200 mg/kg) or HEPN (200 mg/kg) 30 min before exposed to HH. The cardiac function was measured. Serum AST, CK, LDH and cTnI were estimated. Heart tissue oxidase activity, SOD, CAT, GSH-Px, ROS and MDA were estimated. ATP content, Na+/K+-ATPase and Ca2+/Mg2+-ATPase activity was measured. The expression of HIF-1, VEGF, Nrf2, HO-1, Bax, Bcl-2, Caspase-3 was estimated. KEY FINDINGS: Results showed that pretreatment with HEPN or HPN led to a dramatic decrease in the activity of biochemical markers AST, CK, LDH and cTnI in murine serum. They increased the activity of SOD, CAT and GSH-Px and reduced the level of ROS and MDA in the hearts of mice. HEPN and HPN could increase the expression of Nrf2 and OH-1. They could maintain the ATPase activity. The Bax and Caspase-3 expression as well as the ratio of Bax/Bcl-2 were significantly downregulated and the Bcl-2 expression was upregulated by HPN or HEPN compared to the HH group. They may attenuate the HH-induced oxidant stress via free radical scavenging activity. SIGNIFICANCE: The present study showed that the nitronyl nitroxide radical HEPN and HPN may be potential therapeutic agents for treatment of HH-induced cardiac dysfunction.


Assuntos
Antioxidantes/farmacologia , Cardiotônicos/farmacologia , Insuficiência Cardíaca/tratamento farmacológico , Hipóxia/tratamento farmacológico , Óxidos de Nitrogênio/farmacologia , Animais , Antioxidantes/síntese química , Aspartato Aminotransferases/sangue , Aspartato Aminotransferases/genética , ATPase de Ca(2+) e Mg(2+)/genética , ATPase de Ca(2+) e Mg(2+)/metabolismo , Cardiotônicos/síntese química , Caseína Quinases/sangue , Caseína Quinases/genética , Caspase 3/genética , Caspase 3/metabolismo , Catalase/sangue , Catalase/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Glutationa Peroxidase/sangue , Glutationa Peroxidase/genética , Insuficiência Cardíaca/etiologia , Insuficiência Cardíaca/genética , Insuficiência Cardíaca/fisiopatologia , Testes de Função Cardíaca , Heme Oxigenase-1/genética , Heme Oxigenase-1/metabolismo , Hipóxia/complicações , Hipóxia/genética , Hipóxia/fisiopatologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , L-Lactato Desidrogenase/sangue , L-Lactato Desidrogenase/genética , Masculino , Malondialdeído/antagonistas & inibidores , Malondialdeído/metabolismo , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo , Óxidos de Nitrogênio/síntese química , Estresse Oxidativo/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/antagonistas & inibidores , Espécies Reativas de Oxigênio/metabolismo , ATPase Trocadora de Sódio-Potássio/genética , ATPase Trocadora de Sódio-Potássio/metabolismo , Superóxido Dismutase/sangue , Superóxido Dismutase/genética , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo
7.
J Strength Cond Res ; 34(1): 235-248, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30113919

RESUMO

Nunes, RFH, Cidral-Filho, FJ, Flores, LJF, Nakamura, FY, Rodriguez, HFM, Bobinski, F, De Sousa, A, Petronilho, F, Danielski, LG, Martins, MM, Martins, DF, and Guglielmo, LGA. Effects of far-infrared emitting ceramic materials on recovery during 2-week preseason of elite futsal players. J Strength Cond Res 34(1): 235-248, 2020-We investigated the effects of far-infrared emitting ceramic materials (cFIR) during overnight sleep on neuromuscular, biochemical and perceptual markers in futsal players. Twenty athletes performed a 2-week preseason training program and during sleep wore bioceramic (BIO; n = 10) or placebo pants (PL; n = 10). Performance (countermovement jump [CMJ]; squat jump [SJ]; sprints 5, 10, and 15-m) and biochemical markers (tumor necrosis factor alpha-TNF-α, interleukin 10-IL-10, thiobarbituric acid-reactive species [TBARS], carbonyl, superoxide dismutase [SOD], catalase [CAT]) were obtained at baseline and after the 1st and 2nd week of training. Delayed-onset muscle soreness (DOMS) and training strain were monitored throughout. Changes in ΔCMJ and ΔSJ were possibly (60/36/4 [week-1]) and likely (76/22/2 [week-2]) higher in BIO. Both groups were faster in 5-m sprint in week 2 compared with baseline (p = 0.015), furthermore, BIO was likely faster in 10-m sprint (3/25/72 [week 1]). Significant group × time interaction in %ΔTNF-α were observed (p = 0.024 [week-1]; p = 0.021 [week-2]) with values possibly (53/44/3 [week 1]) and likely (80/19/1 [week 2]) higher in BIO. The %ΔIL-10 decreased across weeks compared with baseline (p = 0.019 [week-1]; p = 0.026 [week-2]), showing values likely higher in BIO (81/16/3 [week-1]; 80/17/3 [week-2]). Significant weekly increases in %ΔTBARS (p = 0.001 [week-1]; p = 0.011 [week-2]) and %ΔCarbonyl (p = 0.002 [week-1]; p < 0.001 [week-2]) were observed compared with baseline, showing likely (91/5/4 [week-1]) and possibly (68/30/2 [week-2]) higher changes in BIO. Significant weekly decreases in %ΔSOD were observed compared with baseline (p = 0.046 [week 1]; p = 0.011 [week-2]), and between week 2 and week 1 (p = 0.021), in addition to significant decreases in %ΔCAT compared with baseline (p = 0.070 [week 1]; p = 0.012 [week 2]). Training strain (p = 0.021; very -likely [0/2/98]; week 1) and DOMS was lower in BIO (likely; 7 sessions) with differences over time (p = 0.001). The results suggest that the daily use of cFIR clothing could facilitate recovery, especially on perceptual markers during the early phases of an intensive training period.


Assuntos
Desempenho Atlético/fisiologia , Raios Infravermelhos/uso terapêutico , Condicionamento Físico Humano/fisiologia , Futebol/fisiologia , Adulto , Biomarcadores/sangue , Catalase/sangue , Cerâmica , Vestuário , Método Duplo-Cego , Teste de Esforço , Humanos , Interleucina-10/sangue , Movimento , Força Muscular/fisiologia , Músculo Esquelético/fisiopatologia , Mialgia/etiologia , Mialgia/terapia , Condicionamento Físico Humano/efeitos adversos , Recuperação de Função Fisiológica , Corrida , Sono , Superóxido Dismutase/sangue , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Fator de Necrose Tumoral alfa/sangue , Adulto Jovem
8.
Food Chem ; 309: 125791, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31699554

RESUMO

The aim of this study was to investigate the effect of phosphorylation on the antioxidant activity of (1 → 3)-ß-d-glucan from yeast cell wall. Alkali-insoluble (1 → 3)-ß-d-glucan was extracted from yeast cell wall by an acid-base method. It was found that the purity of the sample was greatly improved after the precipitation was treated with alkali at 90 ℃ and then by acetic acid, which was about 96.5%. Phosphorylated (1 → 3)-ß-d-glucan was prepared. Infrared (IR) spectra and nuclear magnetic resonance spectra (NMR) confirmed the successful introduction of phosphate into glucan. The substitution degree of phosphate was 0.18. The phosphorylated (1 → 3)-ß-d-glucan could significantly increase SOD and CAT contents in serum, liver and brain of mice, and reduce MDA level in serum, liver and brain to a certain extent in vivo. This lays a solid foundation for the research and development of phosphorylated (1 → 3)-ß-d-glucan antioxidant.


Assuntos
Antioxidantes/metabolismo , beta-Glucanas/química , Animais , Antioxidantes/química , Catalase/sangue , Catalase/metabolismo , Parede Celular/química , Parede Celular/metabolismo , Espectroscopia de Ressonância Magnética , Malondialdeído/sangue , Malondialdeído/metabolismo , Camundongos , Fosforilação , Saccharomyces cerevisiae/metabolismo , Espectrofotometria Infravermelho , Superóxido Dismutase/sangue , Superóxido Dismutase/metabolismo
9.
BMC Neurol ; 19(1): 323, 2019 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-31837702

RESUMO

BACKGROUND: In recent years, the role of neuroinflammation and oxidative stress in migraine pathogenesis has achieved considerable interest; however, to date findings are equivocal. Thus, the objective of this study was to investigate biomarkers of oxidative stress in episodic and chronic migraineurs (EM and CM patients) and controls. METHODS: Forty-four patients with EM, 27 individuals with CM and 19 age-sex-matched controls were enrolled. After collecting data on demographic and headache characteristics, blood samples were collected and analyzed to detect serum levels of oxidative stress biomarkers (malondialdehyde (MDA) and nitric oxide (NO)); total antioxidant capacity using Trolox equivalent antioxidant capacity (TEAC) assay; and antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase-1 (GPx-1)). RESULTS: Serum levels of CAT and SOD were significantly lower in the CM group than the EM group and controls. However, serum GPx-1 levels of the CM patients were slightly higher than the EM patients and controls (P-value≤0.001). CM patients had lower mean TEAC values than EM patients and controls. In addition, serum levels of NO and MDA were significantly elevated among subjects with CM compared to EM and control individuals (P-value≤0.001). Pearson correlation analysis revealed negative correlations between the number of days of having headaches per month and serum concentrations of the two antioxidant enzymes CAT (r = - 0.60, P-value< 0.001) and SOD (r = - 0.50, P-value< 0.001) as well as TEAC values (r = - 0.61, P-value< 0.001); however, there were positive correlations between headache days and serum GPx-1 levels (r = 0.46, P-value< 0.001), NO (r = 0.62, P-value< 0.001), and MDA (r = 0.64, P-value< 0.001). CONCLUSION: Present findings highlighted that chronic migraineurs had lower total non-enzymatic antioxidant capacity and higher oxidative stress than episodic migraineurs and control individuals. Although more studies are needed to confirm these data, applying novel prophylactic medications or dietary supplements with antioxidant properties could be promising in migraine therapy.


Assuntos
Biomarcadores/sangue , Transtornos de Enxaqueca/sangue , Estresse Oxidativo/fisiologia , Adulto , Estudos de Casos e Controles , Catalase/sangue , Feminino , Glutationa Peroxidase/sangue , Humanos , Masculino , Malondialdeído/sangue , Pessoa de Meia-Idade , Óxido Nítrico/sangue , Capacidade de Absorbância de Radicais de Oxigênio , Superóxido Dismutase/sangue
10.
Probl Radiac Med Radiobiol ; 24: 480-492, 2019 Dec.
Artigo em Inglês, Ucraniano | MEDLINE | ID: mdl-31841489

RESUMO

OBJECTIVE: to evaluate the influence of processes of lipid peroxidation, and antioxidant protection on the function of external respiration in children-residents of radioactive contaminated territories. MATERIALS AND METHODS: There were examined children of school age, inhabitants of radioactive contaminated ter- ritories (RCT) without respiratory and pulmonary pathology, and patients with bronchial asthma (BA). Examination of the ventilation lung function was performed by the method of pneumotachography according to the analysis of the «flow-volume¼ loop. End products of lipid peroxidation (LPO), reacting with thiobarbituric acid (malondialde- hyde), enzymes-antioxidants - catalase, superoxide dismutase, glutathione peroxidase, glutathione transferase were studied to determine the signs of oxidative stress. RESULTS: Examinations of ventilation lung function in children-residents of RCT without respiratory and pulmonary pathology showed no significant deviations of its parameters beyond the limits of physiological fluctuations; a decrease in bronchial patency at different levels of the bronchial tree was established in children with BA. An increased incidence of bronchial hyperreactivity was noted in both the patients with BA and those who did not have bronchopulmonary pathology. The absence of an increase in the content of LPO products (malondialdehyde) in blood serum of children-residents of RCT with increased activity of catalase and group of glutathione enzymes indi- cates the sufficiency of compensatory possibilities of antioxidant protection. CONCLUSIONS: Studies of correlation between the function of external respiration and markers of oxidative stress determined that the indices of bronchial patency directly correlate with the activity of enzymes-antioxidants of the glutathione group and inversely - with the content of the LPO products in children-residents of RCT. The frequency of bronchospasm inversely correlated with the activity of glutathione group antioxidants. There are inverse correla- tion of 137Cs content in the body with the activity of glutathione transferase and glutathione peroxidase.


Assuntos
Antioxidantes/metabolismo , Asma/diagnóstico , Espasmo Brônquico/diagnóstico , Acidente Nuclear de Chernobyl , Adolescente , Asma/sangue , Asma/fisiopatologia , Biomarcadores/sangue , Espasmo Brônquico/sangue , Espasmo Brônquico/fisiopatologia , Estudos de Casos e Controles , Catalase/sangue , Radioisótopos de Césio/análise , Criança , Feminino , Glutationa Peroxidase/sangue , Glutationa Transferase/sangue , Humanos , Peroxidação de Lipídeos , Pulmão/metabolismo , Pulmão/fisiopatologia , Masculino , Malondialdeído/sangue , Estresse Oxidativo , Exposição à Radiação , Cinza Radioativa , Respiração/efeitos da radiação , Testes de Função Respiratória , Superóxido Dismutase/sangue , Ucrânia
11.
Indian J Med Res ; 150(2): 167-174, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31670272

RESUMO

Background & objectives: High-intensity exercise results in oxidative stress in adult population. Impact of pubertal attainment on high-intensity exercise-induced oxidative stress in sedentary paediatric population has not been investigated in detail. The present study was conducted to investigate the extent of high-intensity exercise-induced oxidative stress in sedentary pre- and post-pubertal boys through estimation of serum thiobarbituric acid reactive substances (TBARS), total thiol content and activities of superoxide dismutase (SOD) and catalase (CAT). Methods: Sixty four sedentary pre-pubertal (n=32, age = 10.21±0.67 yr) and post-pubertal (n=32, age = 15.58±0.47 yr) boys performed incremental treadmill running exercise at 80 per cent of the age predicted maximum heart rate till volitional exhaustion. Blood sample (5 ml) was drawn from each individual before and after the exercise for estimation of oxidative stress markers. Results: Pre-exercise SOD activity and total thiol level showed significant positive relationship with age and were significantly higher in post-pubertal boys. Serum TBARS level, SOD and CAT activities increased while total thiol content decreased in both the groups following exercise. Post-exercise percentage change in TBARS, SOD activity and total thiol level was significantly higher in post-pubertal boys, and these variables had significant positive relationship with age. No significant intergroup variations were noted in CAT activity before or after exercise. Interpretation & conclusions: Extent of post-exercise oxidative stress increased significantly with attainment of puberty. However, baseline and post-exercise antioxidation status also increased significantly as a function of age with pubertal maturation allowing the post-pubertal boys to counter relatively higher oxidative stress more efficiently than their pre-pubertal counterparts. Post-exercise upregulation in CAT activity might not be influenced by age or pubertal maturation in this age group.


Assuntos
Biomarcadores/sangue , Exercício Físico/fisiologia , Estresse Oxidativo/fisiologia , Puberdade/sangue , Adolescente , Catalase/sangue , Criança , Humanos , Masculino , Puberdade/fisiologia , Corrida/fisiologia , Comportamento Sedentário , Superóxido Dismutase/sangue
12.
Dis Markers ; 2019: 6178017, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31737129

RESUMO

Objectives: Obesity has serious consequences such as the onset of metabolic syndrome, type 2 diabetes, atherosclerosis, or cardiovascular complications. The aim of this study was to evaluate the levels of paraoxonase 1 (PON1), lectin-like oxidized LDL receptor-1 (LOX-1), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), and lipid peroxidation processes in the course of obesity. Methods: 28 men took part in the experiment. Fourteen of them were obese; the control group consisted of 14 physically active men without obesity features. The concentrations of malondialdehyde (MDA), PON1, LOX-1, and tumor necrosis factor α (TNFα) as well as the activities of erythrocytic SOD, CAT, and GPx were determined in the study. Results: Statistically significant higher MDA, LOX-1, and TNFα levels were observed in obese subjects. Conversely, lower concentrations of PON1 in obese men were found. Conclusions: An imbalance in oxidation-reduction processes accompanies obesity. Moreover, inflammatory cytokines and atherosclerotic complications are involved in the obesity process. The obtained results suggest that the studied parameters may be independent prognostic markers preceding the development of cardiovascular and metabolic complications in people afflicted with type II obesity.


Assuntos
Arildialquilfosfatase/sangue , Obesidade/sangue , Estresse Oxidativo , Receptores Depuradores Classe E/sangue , Adulto , Estudos de Casos e Controles , Catalase/sangue , Glutationa Peroxidase/sangue , Humanos , Peroxidação de Lipídeos , Masculino , Malondialdeído/sangue , Obesidade/metabolismo , Superóxido Dismutase/sangue , Fator de Necrose Tumoral alfa/sangue
13.
J Therm Biol ; 85: 102420, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31657761

RESUMO

The purpose of the current study was to investigate that effect of duration of thermal stress on growth performance, oxidative stress indices in serum, the expression and localization of ABCG2, and mitochondria ROS production in skeletal muscle, small intestine and immune organs, and then to further reveal correlations between indicators. At 28 days of age, sixty broilers were randomly divided into the control group (25 ±â€¯2 °C; 24 h/day) and the heat stress group (36 ±â€¯2 °C; 8 h/day lasted for 1 week or 2 weeks). Fifteen broilers per group were respectively euthanized, and some samples were respectively collected from the control and the heat stress groups at the end of the 1st week or the 2nd week of heat stress. A typical heat stress response has been observed at this temperature. Compared with the control group, the birds subjected to heat stress at the end of the 1st week reduced (P < 0.05) body weight (BW), average daily feed intake (ADFI), average daily gain (ADG), the activity of serum antioxidant enzyme and content of glutathione (GSH), while increased (P < 0.05) feed conversion ratio (FCR), serum corticosterone and malondialdehyde (MDA) levels. However, when the heat stress lasted for the end of the 2nd week, there was no significant difference (P > 0.05) in ADFI, ADG, FCR and serum contents of corticosterone, MDA and GSH. Regardless of duration of thermal stress, the localization of ABCG2 protein had no change. Moreover, heat stress also did not affect (P > 0.05) the IOD of the ABCG2 positive portion and the expression of the ABCG2 mRNA in the pectorales, crureus, duodenum, jejunum, ileum and spleen, while significantly increased (P < 0.05) the corresponding tissues ROS production at the end of the 1st week of heat stress. In contrast, at the end of the 2nd week of heat stress, IOD of the ABCG2 positive portion and the expression of the ABCG2 mRNA in heat stress group significantly increased (P < 0.05), while the corresponding tissues ROS production had no difference (P > 0.05) compared to the control group. Collectively, duration of thermal stress affects growth performance, serum oxidative stress indices, and the expression of ABCG2 and the ROS production of broiler tissues in a time-dependent manner. There is a negative correlation between the expression of ABCG2 and the ROS production in the corresponding tissues under heat stress.


Assuntos
Galinhas/fisiologia , Transtornos de Estresse por Calor/metabolismo , Transtornos de Estresse por Calor/veterinária , Doenças das Aves Domésticas/metabolismo , Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética , Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo , Animais , Catalase/sangue , Galinhas/sangue , Corticosterona/sangue , Glutationa/sangue , Glutationa Peroxidase/sangue , Glutationa Redutase/sangue , Intestino Delgado/metabolismo , Malondialdeído/sangue , Mitocôndrias/metabolismo , Músculo Esquelético/metabolismo , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismo , Baço/metabolismo , Superóxido Dismutase/sangue , Timo/metabolismo
14.
J Cardiovasc Med (Hagerstown) ; 20(7): 419-426, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31593559

RESUMO

BACKGROUND AND AIM: Cardiovascular diseases (CVDs) are the most frequent causes of death in the world. Inflammation and oxidative damage contribute significantly to the development of atherosclerosis and CVDs. European Food Safety Authority scientific opinion has acknowledged that hydroxytyrosol (3,4-dihydroxyphenylethanol) and derivatives, contained in extra virgin olive oil (EVOO), typically used in Mediterranean diet may play a crucial role in the reduction of the inflammatory pathway and in the prevention of CVDs. The aim of the study was to determine the effect in healthy volunteers of 25 g of phenols-rich EVOO (p-EVOO). METHODS: The clinical study was a randomized, controlled trial to determine the acute effect in the postprandial time of 25 g of p-EVOO. We evaluated nutritional status using anthropometric parameters, body composition, serum metabolites, oxidative stress biomarkers and gene expression of eight genes related to oxidative stress and human inflammasome pathways, lasting 2 h after p-EVOO administration. Twenty-two participants resulted as eligible for the study. RESULTS: A significant reduction of oxidized LDL, malondialdehyde, triglycerides and visceral adiposity index was highlighted (P < 0.05). Significant upregulation of catalase, superoxide dismutase 1 and upstream transcription factor 1 were observed (P < 0.05). CONCLUSION: The current study shows that intake of 25 g of p-EVOO has been able to be modulated, in the postprandial time, the antioxidant profile and the expression of inflammation and oxidative stress-related genes, as superoxide dismutase 1, upstream transcription factor 1 and catalase. We also observed a significant reduction of oxidized LDL, malondialdehyde, triglycerides and visceral adiposity index. We have demonstrated that a daily intake of phenols and antioxidants can reduce the inflammatory pathway and oxidative stress and therefore the risk of atherosclerosis and CVDs. More studies on a larger population are necessary before definitive conclusions can be drawn.Trial registration ClinicalTrials.gov NCT01890070.


Assuntos
Doenças Cardiovasculares/prevenção & controle , Lipoproteínas LDL/sangue , Nutrigenômica/métodos , Azeite de Oliva/metabolismo , Estresse Oxidativo/genética , Fenóis/sangue , Álcool Feniletílico/análogos & derivados , Adolescente , Adulto , Idoso , Doenças Cardiovasculares/sangue , Doenças Cardiovasculares/etiologia , Doenças Cardiovasculares/genética , Catalase/sangue , Catalase/genética , Dieta Saudável , Dieta Mediterrânea , Método Duplo-Cego , Feminino , Humanos , Mediadores da Inflamação/sangue , Masculino , Pessoa de Meia-Idade , Azeite de Oliva/administração & dosagem , Álcool Feniletílico/sangue , Período Pós-Prandial , Fatores de Proteção , Fatores de Risco , Roma , Superóxido Dismutase-1/sangue , Superóxido Dismutase-1/genética , Fatores Estimuladores Upstream/sangue , Fatores Estimuladores Upstream/genética , Adulto Jovem
15.
Toxicol Lett ; 317: 24-44, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31541690

RESUMO

Exposure of humans to xenobiotic mixtures is a continuous state during their everyday routine. However, the majority of toxicological studies assess the in vivo effects of individual substances rather than mixtures. Therefore, our main objective was to evaluate the impact of the 12- and 18-month exposure of rats to a mixture containing 13 pesticides, food, and life-style additives in three dosage levels (i.e. 0.0025 × NOAEL, 0.01 × NOAEL, and 0.05 × NOAEL), on redox biomarkers in blood and tissues. Our results indicate that the exposure to the mixture induces physiological adaptations by enhancing the blood antioxidant mechanism (i.e., increased glutathione, catalase and total antioxidant capacity and decreased protein carbonyls and TBARS) at 12 months of exposure. On the contrary, exposure to the 0.05 × NOAEL dose for 18 months induces significant perturbations in blood and tissue redox profile (i.e., increased carbonyls and TBARS). This study simulates a scenario of real-life risk exposure to mixtures of xenobiotics through a long-term low-dose administration regimen in rats. The results obtained could support, at least in part, the necessity of introducing testing of combined stimuli at reference doses and long term for the evaluation of the risk from exposure to chemicals.


Assuntos
Aditivos Alimentares/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Praguicidas/toxicidade , Xenobióticos/toxicidade , Animais , Biomarcadores/sangue , Catalase/sangue , Relação Dose-Resposta a Droga , Exposição Ambiental/efeitos adversos , Feminino , Glutationa/sangue , Masculino , Nível de Efeito Adverso não Observado , Oxirredução , Carbonilação Proteica/efeitos dos fármacos , Ratos Sprague-Dawley , Medição de Risco , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Fatores de Tempo
16.
Biotech Histochem ; 94(8): 583-591, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31478750

RESUMO

We investigated the potential hepatoprotective effects of misoprostol (MP) on doxorubicin (DOX) induced liver injury in rats using histology and biochemistry. We used 21 male Sprague-Dawley rats divided randomly into three groups: group 1, control; group 2, DOX; group 3, DOX + MP. The control group was injected intraperitoneally (i.p.) with 0.5 ml 0.9% w/v NaCl and given 1 ml 0.9% NaCl orally for 6 days. DOX was administered i.p. as a single dose of 20 mg/kg. MP, 0.2 mg/kg, was given orally for 6 days. Treatment with MP increased high density lipoprotein cholesterol levels and decreased serum alanine aminotransferase, aspartate aminotransferase, low density lipoprotein cholesterol, triglycerides and total cholesterol levels significantly in serum. Increased malondialdehyde level and decreased catalase, glutathione and superoxide dismutase levels caused by DOX were suppressed significantly in liver tissue by MP. DOX + MP reduced loss of body weight. Oxidative stress was decreased, antioxidant activity was increased and histopathological changes were reduced in the DOX + MP group compared to the DOX group. Liver injury caused by DOX was attenuated by MP treatment owing to the antioxidative and anti-apoptotic effects of MP, which might be useful for reducing the severity of DOX induced liver injury.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Doxorrubicina/toxicidade , Misoprostol/farmacologia , Abortivos não Esteroides/farmacologia , Animais , Antibióticos Antineoplásicos/toxicidade , Biomarcadores/sangue , Peso Corporal/efeitos dos fármacos , Catalase/sangue , Glutationa/sangue , Masculino , Estresse Oxidativo/efeitos dos fármacos , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Superóxido Dismutase/sangue
17.
Hum Exp Toxicol ; 38(11): 1235-1243, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31423839

RESUMO

OBJECTIVE: In this study, we aimed to investigate oxidative stress and antioxidant parameter levels in patients with carbon monoxide (CO) poisoning. METHODS: The study was conducted prospectively between March 1, 2015 and April 30, 2016 in the pediatric emergency department. Eligible patients included children aged 0-18 years old with a diagnosis of CO poisoning. To determination of oxidative stress and antioxidant parameter levels, venous blood with heparinized and urine samples were drawn during the admission and after normobaric oxygen (NBO) and hyperbaric oxygen (HBO) treatment. RESULTS: Forty-seven children with CO poisoning for study group and 29 patients as control group were included to the study. Sixteen patients treated with HBO. Basal plasma malondialdehyde levels were found to be significantly higher in the CO poisoning group when compared with the control group (p = 0.019). There is no significant difference in oxidative stress and antioxidant parameter levels except erythrocyte catalase enzyme levels in patients treated with NBO when comparing before and after NBO treatment (p > 0.05). Decreasing of basal erythrocyte catalase enzyme levels were found statistically significant after NBO treatment (p = 0.04). There was no significant difference in oxidative stress and antioxidant parameter levels in patients treated with HBO before and after therapy (p > 0.05). CONCLUSIONS: CO poisoning is associated with increased lipid peroxidation in children immediately after the poisoning. However, both treatment modalities including NBO or HBO do not have a significant effect on oxidative stress or antioxidant parameter levels.


Assuntos
Intoxicação por Monóxido de Carbono/sangue , Intoxicação por Monóxido de Carbono/terapia , Estresse Oxidativo , Oxigenoterapia , Adolescente , Carboxihemoglobina/análise , Catalase/sangue , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Peroxidação de Lipídeos , Masculino , Malondialdeído/sangue , Superóxido Dismutase/sangue
18.
Artigo em Inglês | MEDLINE | ID: mdl-31422162

RESUMO

Deltamethrin (DMN) exposure causes severe damage to the gill and liver tissues of aquatic organisms, as well as neurotoxic effects and metabolic disorders. The goal of the present study was to assess the impacts of DMN toxicity on blood biochemistry, malondialdehyde (MDA) levels, catalase (CAT) levels, behavior disorder, acetylcholinesterase (AChE) activity, histopathology and 8-hydroxy-2-deoxyguanosine (8 OHdG) of brown trout (Salmo trutta fario). Acute concentrations (1.0 and 2.0µg/L) of DMN caused behavioral disorder such as rapid swimming, loss of balance, aggressiveness and increasing in the surface activity and inactivity in brown trout. A significant increase in malondialdehyde (MDA), aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, and a significant decrease in CAT, AChE, blood albumin, and blood total protein content were observed. Histopathologically, both doses of DMN have caused steatosis, necrosis, and degeneration in hepatocytes and hyperemia in the liver. Also, they led to inflammation, adhesion and fusion depending on severe hyperplasia in secondary lamellae, hyperemia and lamellar edema in gill tissues when compared to control group. Additionally, 8-hydroxy-2-deoxyguanosine (8 OHdG) levels at 2.0 µg/L dose of DMN in liver tissues were more severe according to 1.0 µg/L dose of DMN. Finally, different concentrations of DMN led to changes of the histopathology, 8OHdG, the CAT levels, plasma AChE activity, and the serum metabolites, as well as behavioral disorder in brown trout.


Assuntos
Brânquias/metabolismo , Fígado/metabolismo , Nitrilos/toxicidade , Piretrinas/toxicidade , Truta/metabolismo , Poluentes Químicos da Água/toxicidade , Acetilcolinesterase/sangue , Animais , Catalase/sangue , Malondialdeído/sangue , Estresse Oxidativo
19.
Adv Rheumatol ; 59(1): 38, 2019 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-31412940

RESUMO

AIM: SLE is a systemic autoimmune disease generally affecting woman in the reproductive age. It is associated with an altered level of Tregs and oxidative stress while an increase in Tregs, and different antioxidant mechanisms to combat oxidative stress are essential for successful pregnancy. Hence, this study aims to determine the level of CD4+ and CD8+ Tregs and oxidative stress in pregnant lupus patients. METHODS: Ten healthy and 10 pregnant lupus volunteers from the North Indian population, within the age group of 20-30 years were enrolled in the study. All the patients were non-smokers, non-alcoholics and were not associated or undergoing therapy for any other disease. They had a SLEDAI of 37.4 ± 7.32 with 5.2 ± 1.93 years of disease duration. Oxidative stress was determined by measuring the enzyme activity of anti-oxidant enzymes (catalase, superoxide dismutase and glutathione peroxidase) and the level of reduced glutathione and lipids peroxidised, spectrophotometrically. Flowcytometry was performed for immunophenotyping to determine CD8+ and CD4+ Tregs. RESULTS: Elevated CD8+ Tregs and diminished CD4+ Tregs were observed in pregnant lupus patients. Oxidative stress was significantly increased as the activities of anti-oxidant enzymes and level of reduced glutathione was considerably diminished. There was a substantial increase in the amount of lipids peroxidised. CONCLUSION: Pregnant lupus patients undergo considerable level of oxidative stress in comparison to healthy pregnant woman. The decreased level of CD4+ Tregs and an increase in CD8+ Tregs might be another important factor responsible for pregnancy associated complications. Hence, lupus leads to alterations in the necessary conditions for a successful pregnancy, which might eventually cause higher mortality, morbidity and associated complications.


Assuntos
Lúpus Eritematoso Sistêmico/imunologia , Lúpus Eritematoso Sistêmico/metabolismo , Estresse Oxidativo , Complicações na Gravidez/imunologia , Complicações na Gravidez/metabolismo , Linfócitos T Reguladores/citologia , Adulto , Proteínas Sanguíneas/análise , Contagem de Linfócito CD4 , Linfócitos T CD4-Positivos , Linfócitos T CD8-Positivos , Estudos de Casos e Controles , Catalase/sangue , Feminino , Glutationa Peroxidase/sangue , Humanos , Imunofenotipagem , Peroxidação de Lipídeos , Gravidez , Superóxido Dismutase/sangue , Linfócitos T Reguladores/imunologia , Adulto Jovem
20.
Am J Obstet Gynecol ; 221(5): 472.e1-472.e10, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31279844

RESUMO

BACKGROUND: Endometrial cancer is the most common gynecological cancer in the United States. However, no early detection test exists for asymptomatic women at average risk for endometrial cancer. OBJECTIVE: We sought to identify early detection biomarkers for endometrial cancer using prediagnostic serum. STUDY DESIGN: We performed a nested case-control study of postmenopausal women in the Prostate, Lung, Colorectal, and Ovarian cancer screening trial (n = 78,216), including 112 incident endometrial cancer cases and 112 controls. Prediagnostic serum was immunodepleted of high-abundance proteins and digested with sequencing grade porcine trypsin via pressure cycling technology. Quantitative proteomics and phosphoproteomics was performed using high-resolution liquid chromatography-tandem mass spectrometry and highly multiplexed isobaric mass tag combined with basic reversed-phase liquid chromatography. A set of proteins able to predict cancer status was identified with an integrated score assessed by receiver-operator curve analysis. RESULTS: Mean time from blood draw to endometrial cancer diagnosis was 3.5 years (SD, 1.9 years). There were 47 differentially abundant proteins between cases and controls (P < .05). Protein alterations with high predictive potential were selected by regression analysis and compiled into an aggregate score to determine the ability to predict endometrial cancer. An integrated risk score of 6 proteins was directly related to disease incidence in cases with blood draw ≤2 years, >2 years to ≤5 years or >5 years prior to cancer diagnosis. The integrated score distinguished cases from controls with an area under the curve of 0.80 (95% confidence interval, 0.72-0.88). CONCLUSION: An integrated score of 6 proteins using prediagnostic serum from the Prostate, Lung, Colorectal, and Ovarian cancer screening trial distinguishes postmenopausal endometrial cancer cases from controls. Validation is needed to evaluate whether this test can improve prediction or detection of endometrial cancer among postmenopausal women.


Assuntos
Biomarcadores Tumorais/sangue , Detecção Precoce de Câncer , Neoplasias do Endométrio/diagnóstico , Idoso , Caderinas/sangue , Estudos de Casos e Controles , Catalase/sangue , Cromatografia Líquida , Fator B do Complemento/análise , Neoplasias do Endométrio/sangue , Neoplasias do Endométrio/epidemiologia , Feminino , Humanos , Pessoa de Meia-Idade , Complexo de Endopeptidases do Proteassoma/sangue , Proteômica , Ensaios Clínicos Controlados Aleatórios como Assunto , Sensibilidade e Especificidade , Espectrometria de Massas em Tandem , Transferrina/análise , Microglobulina beta-2/sangue
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