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1.
J Transl Med ; 18(1): 411, 2020 11 02.
Artigo em Inglês | MEDLINE | ID: mdl-33138834

RESUMO

BACKGROUND: The SARS-CoV-2 RNA was detected positive again after discharged from hospital in some COVID-19 patients, with or without clinical symptoms such as fever or dry cough. METHODS: 1008 severe COVID-19 patients, with SARS-CoV-2 RNA positive detected with the mixed specimen of nasopharyngeal swab and oropharyngeal swab by real-time fluorescence quantitative PCR (RT-qPCR), were selected to monitor SARS-CoV-2 RNA with the 12 types of specimens by RT-qPCR during hospitalization. All of 20 discharged cases with COVID-19 were selected to detect SARS-CoV-2 RNA in isolation period with 7 types of specimens by RT-qPCR before releasing the isolation period. RESULTS: Of the enrolled 1008 severe patients, the nasopharyngeal swab specimens showed the highest positive rate of SARS-CoV-2 RNA (71.06%), followed by alveolar lavage fluid (66.67%), oropharyngeal swab (30.77%), sputum (28.53%), urine (16.30%), blood (12.5%), stool (12.21%), anal swab (11.22%) and corneal secretion (2.99%), and SARS-CoV-2 RNA couldn't be detected in other types of specimen in this study. Of the 20 discharged cases during the isolation period, the positive rate of SARS-CoV-2 RNA was 30% (6/20): 2 cases were positive in sputum at the eighth and ninth day after discharge, respectively, 1 case was positive in nasopharynx swab at the sixth day after discharge, 1 case was positive in anal swab at the eighth day after discharge, and 1 case was positive in 3 specimens (nasopharynx swab, oropharynx swab and sputum) simultaneously at the fourth day after discharge, and no positive SARS-CoV-2 RNA was detected in other specimens including stool, urine and blood at the discharged patients. CONCLUSIONS: SARS-CoV-2 RNA should be detected in multiple specimens, such as nasopharynx swab, oropharynx swab, sputum, and if necessary, stool and anal swab specimens should be performed simultaneously at discharge when the patients were considered for clinical cure and before releasing the isolation period.


Assuntos
Técnicas de Laboratório Clínico/métodos , Infecções por Coronavirus/diagnóstico , Cavidade Nasal/virologia , Alta do Paciente , Pneumonia Viral/diagnóstico , RNA Viral/sangue , Betacoronavirus/isolamento & purificação , Líquidos Corporais , Hospitalização , Humanos , Pandemias , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes
2.
Dental Press J Orthod ; 25(5): 51-56, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33206829

RESUMO

INTRODUCTION: Nasal septum deviation (NSD) is the most common structural cause of nasal obstruction, affecting around 65-80% of the adult population. Rapid maxillary expansion (RME) is currently used for treatment of maxillary transverse deficiency, but can also influence nasal cavity geometry. OBJECTIVE: The present study aimed at evaluating the changes in NSD by using Cone-Beam Computed Tomography (CBCT) scans in pre-pubertal patients treated with RME. METHODS: This retrospective exploratory study evaluated 20 pre-pubertal patients (mean age 10 ± 2 years) who were treated for transverse maxillary constriction with RME and presented mild/moderate NSD as an incidental finding. The outcome measures were NSD tortuosity and area. These measures were obtained from transverse and coronal views of records taken before and after RME treatment. Intra-rater reliability was also assessed with intraclass correlation coefficient. RESULTS: NSD was mild in thirteen patients (65%) and moderate in seven (35%). NSD tortuosity index did not significantly change over time (mean difference 0.002 mm/year, 95% CI; p = 0.58). NSD area did not significantly change over time (mean difference 2.103 mm2/year, 95% CI; p = 0.38). Intraclass correlation coefficient was 0.73 (95% CI) for NSD tortuosity and 0.84 (95% CI) for NSD area. CONCLUSIONS: NSD tortuosity and area suggested potential changes in NSD with small clinical relevance in pre-pubertal patients who were treated with RME. Additional studies using CBCT scans in larger samples are required to clarify the role of RME in NSD treatment.


Assuntos
Tomografia Computadorizada de Feixe Cônico , Técnica de Expansão Palatina , Criança , Humanos , Maxila , Cavidade Nasal/diagnóstico por imagem , Septo Nasal/diagnóstico por imagem , Reprodutibilidade dos Testes , Estudos Retrospectivos
3.
Trials ; 21(1): 906, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33138848

RESUMO

OBJECTIVES: - To describe the evolution of the SARS-CoV-2 salivary viral load of patients infected with Covid-19, performing 7 days of tri-daily mouthwashes with and without antivirals. - To compare the evolution of the SARS-CoV-2 nasal and salivary viral load according to the presence or absence of antivirals in the mouthwash. TRIAL DESIGN: This is a multi-center, randomised controlled trial (RCT) with two parallel arms (1:1 ratio). PARTICIPANTS: Inclusion criteria - Age: 18-85 years old - Clinical diagnosis of Covid-19 infection - Clinical signs have been present for less than 8 days - Virological confirmation - Understanding and acceptance of the trial - Written agreement to participate in the trial Exclusion criteria - Pregnancy, breastfeeding, inability to comply with protocol, lack of written agreement - Patients using mouthwash on a regular basis (more than once a week) - Patient at risk of infectious endocarditis - Patients unable to answer questions - Uncooperative patient The clinical trial is being conducted with the collaboration of three French hospital centers: Hospital Center Emile Roux (Le Puy en Velay, France), Clinic of the Protestant Infirmary (Lyon, France) and Intercommunal Hospital Center (Mont de Marsan, France). INTERVENTION AND COMPARATOR: Eligible participants will be allocated to one of the two study groups. Intervention group: patients perform a tri-daily mouthwash with mouthwash containing antivirals (ß-cyclodextrin and Citrox®) for a period of 7 days. CONTROL GROUP: patients perform a tri-daily mouthwash with a placebo mouthwash for a period of 7 days. MAIN OUTCOMES: Primary Outcome Measures: Change from Baseline amount of SARS-CoV-2 in salivary samples at 4 and 9 hours, 1, 2, 3, 4, 5 and 6 days. Real-time PCR assays are performed to assess salivary SARS-CoV 2 viral load. SECONDARY OUTCOME MEASURES: Change from Baseline amount of SARS-CoV-2 virus in nasal samples at 6 days. Real-time PCR assays are performed to assess nasal SARS-CoV-2 viral load. RANDOMISATION: Participants meeting all eligibility requirements are allocated to one of the two study arms (mouthwash with ß-cyclodextrin and Citrox® or mouthwash without ß-cyclodextrin and Citrox®) in a 1:1 ratio using simple randomisation with computer generated random numbers. BLINDING (MASKING): Participants, doctors and nurses caring for participants, laboratory technicians and investigators assessing the outcomes will be blinded to group assignment. NUMBERS TO BE RANDOMISED (SAMPLE SIZE): Both the intervention and control groups will be composed of 103 participants, so the study will include a total of 206 participants. TRIAL STATUS: The current protocol version is 6, August 4th, 2020. Recruitment began on April 6, 2020 and is anticipated to be complete by April 5, 2021. As of October 2, 2020, forty-two participants have been included. TRIAL REGISTRATION: This trial was registered on 20 April 2020 at www.clinicaltrials.gov with the number NCT04352959 . FULL PROTOCOL: The full protocol is attached as an additional file, accessible from the Trials website (Additional file 1). In the interest in expediting dissemination of this material, the familiar formatting has been eliminated; this Letter serves as a summary of the key elements of the full protocol." The study protocol has been reported in accordance with the Standard Protocol Items: Recommendations for Clinical Interventional Trials (SPIRIT) guidelines (Additional file 2)."


Assuntos
Betacoronavirus , Técnicas de Laboratório Clínico/métodos , Infecções por Coronavirus , Antissépticos Bucais , Cavidade Nasal/virologia , Pandemias , Pneumonia Viral , Saliva/virologia , Adulto , Antivirais/administração & dosagem , Antivirais/efeitos adversos , Betacoronavirus/efeitos dos fármacos , Betacoronavirus/isolamento & purificação , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/terapia , Monitoramento de Medicamentos/métodos , Feminino , Humanos , Masculino , Antissépticos Bucais/administração & dosagem , Antissépticos Bucais/efeitos adversos , Pneumonia Viral/diagnóstico , Pneumonia Viral/terapia , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento , Carga Viral , beta-Ciclodextrinas/administração & dosagem , beta-Ciclodextrinas/efeitos adversos
4.
Otolaryngol Clin North Am ; 53(6): 1139-1151, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33039097

RESUMO

This review summarizes the challenges and adaptations that have taken place in rhinology and facial plastics in response to the ongoing coronavirus disease-19 pandemic. In particular, the prolonged exposure and manipulation of the nasal and oral cavities portend a high risk of viral transmission. We discuss evidence-based recommendations to mitigate the risk of viral transmission through novel techniques and device implementation as well as increasing conservative management of certain pathologies.


Assuntos
Infecções por Coronavirus/prevenção & controle , Procedimentos Cirúrgicos Eletivos/estatística & dados numéricos , Controle de Infecções/métodos , Pandemias/prevenção & controle , Pneumonia Viral/prevenção & controle , Rinoplastia/métodos , Ritidoplastia/métodos , Infecções por Coronavirus/epidemiologia , Feminino , Humanos , Transmissão de Doença Infecciosa do Paciente para o Profissional/prevenção & controle , Masculino , Boca/virologia , Cavidade Nasal/virologia , Saúde do Trabalhador , Pandemias/estatística & dados numéricos , Segurança do Paciente , Pneumonia Viral/epidemiologia , Rinoplastia/efeitos adversos , Ritidoplastia/efeitos adversos , Gestão da Segurança/métodos
5.
Aust J Gen Pract ; 49(11): 728-732, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33123703

RESUMO

BACKGROUND: General practitioners (GPs) have some of the highest rates of mortality from COVID-19 among healthcare workers. SARS-CoV-2 has unique properties that place GPs at particular risk. OBJECTIVE: The aim of this article is to discuss the nose-related features of SARS-CoV-2 that place GPs at risk, and to make recommendations pertinent to the safety and protection of primary healthcare physicians. DISCUSSION: The highest viral load of SARS-CoV-2 is in the nose and nasopharynx. It is often highest early in the illness, before the development of symptoms. Further, SARS-CoV-2 replicates and continues to shed in the nasopharynx long after the virus is no longer detectable in the lower respiratory tract. This places any physician performing examinations on, or procedures involving, the upper respiratory tract at risk for contracting COVID-19. New-onset hyposmia and dysgeusia are indicators for COVID-19 and should be included in screening protocols.


Assuntos
Betacoronavirus , Infecções por Coronavirus , Clínicos Gerais/estatística & dados numéricos , Controle de Infecções , Cavidade Nasal/virologia , Nasofaringe/virologia , Transtornos do Olfato/virologia , Pandemias , Pneumonia Viral , Austrália , Betacoronavirus/isolamento & purificação , Betacoronavirus/fisiologia , Infecções por Coronavirus/mortalidade , Infecções por Coronavirus/prevenção & controle , Infecções por Coronavirus/transmissão , Infecções por Coronavirus/virologia , Transmissão de Doença Infecciosa/prevenção & controle , Humanos , Controle de Infecções/instrumentação , Controle de Infecções/métodos , Controle de Infecções/normas , Mucosa Nasal/metabolismo , Exposição Ocupacional/prevenção & controle , Exposição Ocupacional/estatística & dados numéricos , Pandemias/prevenção & controle , Equipamento de Proteção Individual/provisão & distribução , Pneumonia Viral/mortalidade , Pneumonia Viral/prevenção & controle , Pneumonia Viral/transmissão , Pneumonia Viral/virologia , Guias de Prática Clínica como Assunto , Atenção Primária à Saúde/normas , Gestão de Riscos , Carga Viral
6.
PLoS One ; 15(10): e0241100, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33108384

RESUMO

Both polyester and foam nasal swabs were collected from convalescent COVID-19 patients at a single visit and stored in viral transport media (VTM), saline or dry. Sensitivity of each swab material and media combination were estimated, three by three tables were constructed to measure polyester and foam concordance, and cycle threshold (Ct) values were compared. 126 visits had polyester and foam swabs stored in viral transport media (VTM), 51 had swabs stored in saline, and 63 had a foam swab in VTM and a polyester swab stored in a dry tube. Polyester and foam swabs had an estimated sensitivity of 87.3% and 94.5% respectively in VTM, 87.5% and 93.8% respectively in saline, and 75.0% and 90.6% respectively for dry polyester and foam VTM. Polyester and foam Ct values were correlated, but polyester showed decreased performance for cases with a viral load near the detection threshold and higher Ct values on average.


Assuntos
Betacoronavirus/isolamento & purificação , Técnicas de Laboratório Clínico , Convalescença , Infecções por Coronavirus/virologia , Cavidade Nasal/virologia , Pandemias , Pneumonia Viral/virologia , Poliésteres , Poliuretanos , Manejo de Espécimes/instrumentação , Adulto , Betacoronavirus/genética , Infecções por Coronavirus/diagnóstico , Meios de Cultura , Equipamentos Descartáveis/provisão & distribução , Feminino , Pessoal de Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Pneumonia Viral/diagnóstico , RNA Viral/análise , Distribuição Aleatória , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Solução Salina , Sensibilidade e Especificidade , Manejo de Espécimes/métodos , Carga Viral
7.
Indian J Dent Res ; 31(4): 647-651, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33107472

RESUMO

Headache is a common clinical problem, and appropriate diagnosis and management are a challenge for oral physician. Any minor anatomical variation within the nasal cavity may lead to mucosal contact point, which may be an etiological factor for causing headache and often left behind by clinician during preliminary evaluation of patients with headache or facial pain, resulting in misdiagnosis and inappropriate treatment. This article is an attempt to present a case of rhinogenic contact point headache which may be mistaken for a toothache initially leading to incorrect diagnosis and irrelevant treatment. A thorough, accurate and comprehensive history taking and a complete clinical and general physical examination result in appropriate diagnosis of the clinical situation.


Assuntos
Dor Facial , Cefaleia , Variação Anatômica , Dor Facial/diagnóstico , Dor Facial/etiologia , Cefaleia/diagnóstico , Cefaleia/etiologia , Humanos , Cavidade Nasal , Odontalgia
8.
Wiad Lek ; 73(8): 1626-1631, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33055323

RESUMO

OBJECTIVE: The aim: Grounding on the electronic microscopy of PT make conclusions about the tonsil activity in adults depending on the accompanied pathology of nose and PS. PATIENTS AND METHODS: Materials and methods: Ultramicroscopic examination of 111 patients with PT hypertrophy aged 18-55 was done. Depending on the nose and PS pathology (inflammatory, non-inflammatory) patients' PS biopsic materials were distributed into 2 groups: 58 cases on inflammatory and 53 on non-inflammatory background. The control group consisted of 24 patients aged 18 without nose and PS pathology. The images of ultrathin PT sections were received with the help of transmission electronic microscope PEM - 125 with digital camera (SELMI, Sumy). RESULTS: Results: Great variations in PT cell condition, depending on the pathology were distinguished. Features of the adenoiditis in inflammatory and non-inflammatory diseases of nose and PS were proved. CONCLUSION: Conclusions: 1. PT ultra-microscopy in control group shows great activity of lymphocytes and high energy exchange of cells, with prevailing B-lymphocyte population. 2. The complex of PT ultra structural changes while nose and PS inflammatory diseases shows the activation of immune reaction in competent cells with T-lymphocyte increase in patients older than 25, which witnesses chronic inflammation. 3. In group with nose and PT non-inflammatory diseases, activity of PT B-lymphocytes is unchanged accompanied by the T-lymphocytes growth, which is also characteristic for chronic inflammation.


Assuntos
Tonsila Faríngea , Seios Paranasais , Adolescente , Adulto , Humanos , Inflamação , Pessoa de Meia-Idade , Cavidade Nasal , Nariz , Adulto Jovem
9.
Artigo em Chinês | MEDLINE | ID: mdl-33040514

RESUMO

We reported a case of a 43-year-old female patient with nasal Sjogren's syndrome(SS). She complained of dry and tingling nose for 5 months. Physical examination: bilateral nasal stenosis, swelling of the mucosa at the front of the nasal septum, dry oral mucosa, and strawberry tongue. Sinus CT showed: bilateral nasal cavity stenosis, nasal septal mucosa and bilateral nasal mucosa hypertrophy. Salivary gland dynamic imaging: the maximum excretion percentage of the left salivary gland is 4.05%, and the maximum excretion percentage of the right salivary gland is 1.81%. Labial gland biopsy showed that the salivary gland lobular structure existed, and multiple lymphocyte foci(>50/each foci) were seen in the focal interstitium, which was consistent with the labial gland performance of SS. Immunohistochemistry showed: CD3+, CD20+, AE1/AE3+, Ki-67+, IgG+. After symptomatic treatment, the patient's dry nose and tingling symptoms disappeared. The swelling of the nasal mucosa disappeared, and the bilateral nasal cavity was well ventilated. Follow-up for half a year, no symptoms of nasal manifestations about Sjogren's syndrome has occurred.


Assuntos
Síndrome de Sjogren , Adulto , Feminino , Humanos , Cavidade Nasal/diagnóstico por imagem , Mucosa Nasal , Septo Nasal , Glândulas Salivares
10.
Vestn Otorinolaringol ; 85(4): 80-84, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32885643

RESUMO

This article presents the clinical case of a woman aged 61 years with massive bilateral synechial obstruction of the nasal cavity after anterior tamponade with clinical manifestations in the form of a significant deterioration in nasal breathing, nasal congestion, postnasal nasal congestion and recurrent sinusitis. The stages of diagnosis of pathology, including differential, are presented and the most rational method of surgical treatment is selected.


Assuntos
Obstrução Nasal , Sinusite , Endoscopia , Feminino , Humanos , Pessoa de Meia-Idade , Cavidade Nasal , Nariz
11.
Nat Commun ; 11(1): 4400, 2020 09 02.
Artigo em Inglês | MEDLINE | ID: mdl-32879306

RESUMO

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly transmitted through the respiratory route, but potential extra-respiratory routes of SARS-CoV-2 transmission remain uncertain. Here we inoculated five rhesus macaques with 1 × 106 TCID50 of SARS-CoV-2 conjunctivally (CJ), intratracheally (IT), and intragastrically (IG). Nasal and throat swabs collected from CJ and IT had detectable viral RNA at 1-7 days post-inoculation (dpi). Viral RNA was detected in anal swabs from only the IT group at 1-7 dpi. Viral RNA was undetectable in tested swabs and tissues after intragastric inoculation. The CJ infected animal had a higher viral load in the nasolacrimal system than the IT infected animal but also showed mild interstitial pneumonia, suggesting distinct virus distributions. This study shows that infection via the conjunctival route is possible in non-human primates; further studies are necessary to compare the relative risk and pathogenesis of infection through these different routes in more detail.


Assuntos
Betacoronavirus/fisiologia , Túnica Conjuntiva/virologia , Infecções por Coronavirus/virologia , Modelos Animais de Doenças , Pneumonia Viral/virologia , Animais , Anticorpos Antivirais , Betacoronavirus/genética , Betacoronavirus/isolamento & purificação , Infecções por Coronavirus/patologia , Intestino Grosso/virologia , Pulmão/patologia , Pulmão/virologia , Macaca mulatta , Masculino , Cavidade Nasal/virologia , Pandemias , Pneumonia Viral/patologia , RNA Viral/análise , RNA Viral/genética , Traqueia/virologia , Carga Viral , Replicação Viral
12.
J Med Microbiol ; 69(10): 1253-1261, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32909934

RESUMO

Introduction. Staphylococcus aureus is a skin and mucous commensal bacterium of warm-blooded animals. In humans, the nose is the main ecological niche of S. aureus, and nasal carriage is a risk factor for developing an endogenous infection. S. aureus nasal colonization is a multifactorial process, involving inter-species interactions among the nasal microbiota.Aims. The objectives of this study were to characterize the microbiota of carriers and non-carriers of S. aureus and to demonstrate the importance of inter-species relationships in the adhesion of S. aureus, a key step in nasal colonization.Methodology. First, we characterized the nasal microbiota from 30 S. aureus carriers and non-carriers by a culturomic approach. We then evaluated the adhesion of S. aureus, first alone and then along with other bacteria of the nasal microbiota. To do that, we used an in vitro model to measure the interactions among bacteria in the presence of epithelial cells.Results. Analysis of the nasal microbiota of the carriers and non-carriers of S. aureus made it possible to observe that each microbiota has specific features in terms of composition. However, this composition differs significantly between carriers and non-carriers mainly through two bacterial groups: coagulase-negative staphylococci and corynebacteria. In a second part, adhesion of S. aureus to epithelial cells showed competition between S. aureus and these bacteria, suggesting a limitation of nasal colonization by S. aureus.Conclusion. These findings demonstrate the existence of a negative correlation between S. aureus and other species which inhibits adhesion and could limit nasal colonization.


Assuntos
Aderência Bacteriana/fisiologia , Mucosa Nasal/metabolismo , Staphylococcus aureus/metabolismo , Adulto , Bactérias , Portador Sadio/microbiologia , Células Epiteliais/metabolismo , Células Epiteliais/microbiologia , Feminino , Humanos , Masculino , Microbiota , Cavidade Nasal/microbiologia , Mucosa Nasal/microbiologia , Nariz/microbiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/patogenicidade
14.
Bull Cancer ; 107(9): 934-945, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32896369

RESUMO

The esthesioneuroblastoma (ENB) is characterized as a rare malignant sinonasal tumor of neuroectodermal origin. Its starting point is the olfactory epithelium located in the upper part of the nasal cavities. Different nomenclatures have been proposed, but the most common are "esthesioneuroblastoma" and "olfactory neuroblastoma". ENBs have a bimodal distribution and mainly occur in teenagers, young adults and people aged 50-60. It is a very rare tumor in pediatrics since only around 100 cases have been reported so far. Within ENBs, we can distinguish tumors with different biological behavior ranging from localized forms with slow evolution to aggressive and metastatic forms at onset. In addition, precisely diagnosing undifferentiated tumors and distinguishing them from other etiologies of sinonasal tumors are sometime difficult. Added to its very low incidence, these characteristics make the study of ENB complicated. The standard treatment currently includes broad surgery followed by radiation therapy in localized resectable tumors. Neoadjuvant chemotherapy is indicated in large unresectable tumors and in metastatic forms. However, in certain indications, such as high-grade operable tumors, the role of perioperative chemotherapy remains to be defined. The objective of this analysis is to detail current knowledge regarding ENBs' epidemiological, biological, clinical and radiological characteristics as well as how to manage ENB in young patients.


Assuntos
Estesioneuroblastoma Olfatório/diagnóstico , Estesioneuroblastoma Olfatório/terapia , Cavidade Nasal , Neoplasias Nasais/diagnóstico , Neoplasias Nasais/terapia , Adolescente , Criança , Humanos , Estadiamento de Neoplasias , Adulto Jovem
15.
Int J Infect Dis ; 99: 352-354, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32791207

RESUMO

The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction, by comparing the efficacy of RT-qPCR in several direct approaches versus the gold standard RNA extraction, in the detection of SARS-CoV-2 in laboratory samples, as well as in clinical oro-nasopharyngeal SARS-CoV-2 swabs. The findings showed an advantage for the extraction procedure; however a direct no-buffer approach might be an alternative, since it identified more than 60% of positive clinical specimens.


Assuntos
Betacoronavirus/genética , Infecções por Coronavirus/diagnóstico , Pneumonia Viral/diagnóstico , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Animais , Chlorocebus aethiops , Estudos de Viabilidade , Humanos , Cavidade Nasal/virologia , Pandemias , RNA Viral/genética , Células Vero
16.
Int J Infect Dis ; 99: 397-402, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32800855

RESUMO

In routine clinical practice, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is determined by reverse-transcription PCR (RT-PCR). In the current pandemic, a more rapid and high-throughput method is in growing demand. Here, we validated the performance of a new antigen test (LUMIPULSE) based on chemiluminescence enzyme immunoassay. A total of 313 nasopharyngeal swabs (82 serial samples from 7 infected patients and 231 individual samples from 4 infected patients and 215 uninfected individuals) were analyzed for SARS-CoV-2 with quantitative RT-PCR (RT-qPCR) and then subjected to LUMIPULSE. We determined the cutoff value for antigen detection using receiver operating characteristic curve analysis and compared the performance of the antigen test with that of RT-qPCR. We also compared the viral loads and antigen levels in serial samples from seven infected patients. Using RT-qPCR as the reference, the antigen test exhibited 55.2% sensitivity and 99.6% specificity, with a 91.4% overall agreement rate (286/313). In specimens with > 100 viral copies and between 10 and 100 copies, the antigen test showed 100% and 85% concordance with RT-qPCR, respectively. This concordance declined with lower viral loads. In the serially followed patients, the antigen levels showed a steady decline, along with viral clearance. This gradual decline was in contrast with the abrupt positive-to-negative and negative-to-positive status changes observed with RT-qPCR, particularly in the late phase of infection. In summary, the LUMIPULSE antigen test can rapidly identify SARS-CoV-2-infected individuals with moderate to high viral loads and may be helpful for monitoring viral clearance in hospitalized patients.


Assuntos
Técnicas de Laboratório Clínico , Infecções por Coronavirus/diagnóstico , Pneumonia Viral/diagnóstico , Reação em Cadeia da Polimerase em Tempo Real , Betacoronavirus , Técnicas de Laboratório Clínico/métodos , Humanos , Técnicas Imunoenzimáticas , Medições Luminescentes , Cavidade Nasal/virologia , Pandemias , Reação em Cadeia da Polimerase em Tempo Real/métodos , Sensibilidade e Especificidade , Carga Viral
17.
J Virol Methods ; 285: 113948, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32783913

RESUMO

The COVID-19 pandemic has led to a worldwide shortage of nasopharyngeal swabs and universal transport media. This study evaluated a combined oropharynx/nares (OP/Na) sample collection using two readily-available non-flocked swabs, transported in phosphate-buffered saline, and demonstrates equivalent performance in SARS-CoV-2 detection compared to a previously-validated OP/Na collection kit.


Assuntos
Betacoronavirus , Técnicas de Laboratório Clínico , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/virologia , Cavidade Nasal/virologia , Orofaringe/virologia , Pneumonia Viral/diagnóstico , Pneumonia Viral/virologia , Idoso , Idoso de 80 Anos ou mais , Betacoronavirus/genética , Betacoronavirus/imunologia , Betacoronavirus/isolamento & purificação , Técnicas de Laboratório Clínico/métodos , Técnicas de Laboratório Clínico/normas , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Reação em Cadeia da Polimerase , Sensibilidade e Especificidade , Manejo de Espécimes
18.
Medicine (Baltimore) ; 99(30): e21183, 2020 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-32791692

RESUMO

INTRODUCTION: Allergic rhinitis (AR) is an immunoglobulin E (Ig E)-mediated inflammatory disease. Intranasal phototherapy is a promising treatment modality because it has a profound immunosuppressive effect, but the evidence of its use for AR is insufficient. Therefore, rigorously designed randomized controlled trials (RCTs) are needed. Our objective is to describe the protocol for an RCT to assess the effects and safety of intranasal phototherapy for the treatment of AR. METHODS AND ANALYSIS: This is a study protocol for a single-center, randomized, parallel (acupuncture-controlled), open-label, investigator-initiated, pilot study. A total of 80 patients with AR will be randomly assigned to the intranasal phototherapy or acupuncture group at a 1:1 ratio. The participants will receive intranasal phototherapy with medical or acupuncture treatment for 20 minutes, 3 times a week for 4 weeks. The primary outcome will be the mean change in the total nasal symptom score (TNSS) from baseline to 4 weeks. The secondary outcomes will include the Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ) score, Nasal Endoscopy Index, total serum immunoglobulin E (Ig E) level and eosinophil count. DISCUSSION: The findings of this study will provide the basis for the design and implementation of RCTs investigating the effects and safety of intranasal phototherapy for AR. Additionally, it will provide preliminary evidence of intranasal phototherapy for use in AR. TRIAL REGISTRATION: This study was registered at the Korean National Clinical Trial Registry, Clinical Research Information Service (KCT0004079).


Assuntos
Eosinófilos , Terapia com Luz de Baixa Intensidade , Rinite Alérgica/terapia , Terapia por Acupuntura , Humanos , Imunoglobulina E/sangue , Contagem de Leucócitos , Terapia com Luz de Baixa Intensidade/efeitos adversos , Cavidade Nasal , Projetos Piloto , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto , Rinite Alérgica/sangue , Índice de Gravidade de Doença , Método Simples-Cego
19.
Int J Immunopathol Pharmacol ; 34: 2058738420941757, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32799596

RESUMO

Public health measures are essential to protect against COronaVIrus Disease 2019 (COVID-19). The nose and the mouth represent entry portals for the COVID 19. Saline Nasal Irrigations (SNIs) can reduce the viral load in the nasal cavities. Oral rinse with antimicrobial agents is efficacious in reducing the viral load in oral fluids. We advocate the inclusion of SNIs and ethanol oral rinses as additional measures to the current public health measures, to prevent and control the transmission of any respiratory infectious disease, including COVID-19.


Assuntos
Infecções por Coronavirus , Etanol/uso terapêutico , Boca/virologia , Cavidade Nasal/virologia , Pandemias , Pneumonia Viral , Irrigação Terapêutica/métodos , Betacoronavirus/efeitos dos fármacos , Betacoronavirus/fisiologia , Infecções por Coronavirus/prevenção & controle , Infecções por Coronavirus/virologia , Transmissão de Doença Infecciosa/prevenção & controle , Humanos , Antissépticos Bucais/uso terapêutico , Pandemias/prevenção & controle , Pneumonia Viral/prevenção & controle , Pneumonia Viral/virologia , Carga Viral/efeitos dos fármacos
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