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1.
BMC Infect Dis ; 19(1): 622, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31307416

RESUMO

BACKGROUND: Cell-surface mucins are expressed in apical epithelial cells of the respiratory tract, and contribute a crucial part of the innate immune system. Despite anti-inflammatory or antiviral functions being revealed for certain cell-surface mucins such as MUC1, the roles of other mucins are still poorly understood, especially in viral infections. METHODS: To further identify mucins significant in influenza infection, we screened the expression of mucins in human nasal epithelial cells infected by H3N2 influenza A virus. RESULTS: We found that the expression of MUC15 was significantly upregulated upon infection, and specific only to active infection. While MUC15 did not interact with virus particles or reduce viral replication directly, positive correlations were observed between MUC15 and inflammatory factors in response to viral infection. Given that the upregulation of MUC15 was only triggered late into infection when immune factors (including cytokines, chemokines, EGFR and phosphorylated ERK) started to peak and plateau, MUC15 may potentially serve an immunomodulatory function later during influenza viral infection. CONCLUSIONS: Our study revealed that MUC15 was one of the few cell-surface mucins induced during influenza infection. While MUC15 did not interact directly with influenza virus, we showed that its increase coincides with the peak of immune activation and thus MUC15 may serve an immunomodulatory role during influenza infection.


Assuntos
Vírus da Influenza A Subtipo H3N2/fisiologia , Influenza Humana/patologia , Mucinas/metabolismo , Animais , Células Cultivadas , Quimiocinas/metabolismo , Citocinas/metabolismo , Cães , Células Epiteliais/classificação , Células Epiteliais/metabolismo , Receptores ErbB/metabolismo , Humanos , Influenza Humana/metabolismo , Células Madin Darby de Rim Canino , Mucinas/antagonistas & inibidores , Mucinas/genética , Cavidade Nasal/citologia , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/farmacologia , Regulação para Cima , Replicação Viral/efeitos dos fármacos
2.
Toxicology ; 395: 9-14, 2018 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-29307546

RESUMO

BACKGROUND AND AIMS: It is recognized that the air pollution is associated with the pathogenesis of airway diseases. This study aims to elucidate the role of the 3-methyl-4-nitrophenol (PNMC), one of the components of diesel-exhaust particles, in compromising the airway epithelial barrier integrity. METHODS: A549 cells, an airway epithelial cell line, were cultured to monolayers to be used as an in vitro epithelial barrier model. BALB/c mice were treated with nasal drops containing PNMC to test the effects of PNMC on alternating the airway epithelial barrier functions. RESULTS: Exposure of mice to PNMC induced nasal epithelial cell apoptosis and increased the permeability of the nasal epithelial barrier. PNMC increased casp8 and casp3 activities in nasal epithelial cells. Exposure to PNMC up regulated Fas and FasL expression in airway epithelial cells. Inhibition of caspase abolished the PNMC-induced airway epithelial barrier dysfunction. CONCLUSION: Exposure of airway mucosa to PNMC induces epithelial cell apoptosis and compromises the epithelial barrier function, which can be prevented by the inhibition of caspases.


Assuntos
Poluentes Atmosféricos/toxicidade , Barreira Alveolocapilar/efeitos dos fármacos , Cresóis/toxicidade , Epitélio/efeitos dos fármacos , Sistema Respiratório/efeitos dos fármacos , Células A549 , Animais , Caspase 3/biossíntese , Caspase 3/genética , Caspase 8/biossíntese , Caspase 8/genética , Células Epiteliais/efeitos dos fármacos , Proteína Ligante Fas/biossíntese , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Cavidade Nasal/citologia , Cavidade Nasal/efeitos dos fármacos , Material Particulado/toxicidade , Sistema Respiratório/patologia , Regulação para Cima/efeitos dos fármacos , Emissões de Veículos/toxicidade
3.
Gene Expr Patterns ; 27: 46-55, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29122676

RESUMO

The nose is the central feature of the amniote face. In adults, the nose is a structurally and functionally complex organ that consists of bone, cartilage, glands and ducts. In an ongoing expression screen in our lab, we found several novel markers for specific tissues in the nasal region. Here, using in situ hybridization expression experiments, we report that Alx1, Ap-2ß, Crispld1, Eya4, Moxd1, and Penk have tissue specific expression during murine nasal development. At E11.5, we observed that Alx1, Ap-2ß, Crispld1, and Eya4 are expressed in the medial and lateral nasal prominences. We found that Moxd1 and Penk are expressed in the lateral nasal prominences. At E15.5, Alx1 is expressed in nasal septum. Ap-2ß and Crispld1 are expressed in nasal glands and cartilages. Eya4 is expressed in olfactory epithelium. Intriguingly at E15.5 Moxd1 is expressed in all the nasal cartilage while the expression of Penk is restricted to chondrocytes contributing to the posterior nasal septum. The expression domains reported here suggest that these genes warrant functional studies to determine their role in nasal capsule morphogenesis.


Assuntos
Condrócitos/metabolismo , Embrião de Mamíferos/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Cavidade Nasal/metabolismo , Mucosa Olfatória/metabolismo , Animais , Células Cultivadas , Condrócitos/citologia , Embrião de Mamíferos/citologia , Feminino , Proteínas de Homeodomínio/metabolismo , Camundongos , Cavidade Nasal/citologia , Mucosa Olfatória/citologia , Transativadores/metabolismo , Fator de Transcrição AP-2/metabolismo
4.
J Speech Lang Hear Res ; 60(11): 3171-3176, 2017 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-29098280

RESUMO

Purpose: This research note describes an adapted experimental methodology to administer an exogenous agent to the larynx and upper airway of awake animals. The exogenous agent could be a perturbation. In the current study, the agent was isotonic saline. Isotonic saline was selected because it is safe, of similar composition to extracellular fluid, and used in voice studies. The described approach allowed large animals such as pigs to be comfortably restrained without chemical sedation or anesthesia for extended periods while receiving the agent. Method: Six Sinclair pigs were successfully trained with positive reinforcement to voluntarily enter and then be restrained in a Panepinto Sling. Once restrained, the pigs accepted a nose cone that delivered nebulized isotonic saline. This procedure was repeated 3 times per day for 20 days. At the end of the study, the larynx and airway tissues were excised and examined using histology and transmission electron microscopy. Results: Pathology related to the procedure (i.e., nebulized inhaled isotonic saline or stress) was not identified in any examined tissues. Conclusions: This methodology allowed for repeated application of exogenous agents to awake, unstressed animals. This method can be used repeatedly in the laboratory to test various therapeutics for safety, toxicity, and dosage. Future studies will specifically manipulate the type of agent to further our understanding of laryngeal pathobiology.


Assuntos
Administração Intranasal/instrumentação , Administração Intranasal/métodos , Laringe/efeitos dos fármacos , Fármacos do Sistema Respiratório/administração & dosagem , Cloreto de Sódio/administração & dosagem , Porco Miniatura , Animais , Feminino , Soluções Isotônicas/administração & dosagem , Laringe/citologia , Laringe/ultraestrutura , Pulmão/citologia , Pulmão/efeitos dos fármacos , Pulmão/ultraestrutura , Microscopia Eletrônica de Transmissão , Modelos Animais , Cavidade Nasal/citologia , Cavidade Nasal/efeitos dos fármacos , Cavidade Nasal/ultraestrutura , Restrição Física/instrumentação , Estresse Psicológico/prevenção & controle , Suínos
5.
Anat Histol Embryol ; 46(6): 592-599, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28960404

RESUMO

Ten apparently healthy, adult laughing doves were used to document detailed histological, histochemical and surface ultrastructural features of the nasal cavity and to investigate the structure-function relationship of the nasal cavity in this species. We observed that the nasal cavity of the laughing dove was composed of three main regions: nasal vestibule, respiratory and olfactory. Each region presented a characteristic epithelial lining. The epithelium varied along the nasal vestibule from keratinized stratified squamous rostrally to non-keratinized stratified squamous in the middle and stratified cuboidal in the caudal region of the nasal vestibule. The respiratory region was lined with pseudostratified columnar epithelium and was initially devoid of both goblet cells and cilia, but cilia then appeared and increased gradually in number close to the olfactory region. The caudal part of the respiratory region presented a stratified cuboidal epithelium. Strong alcianophilic, intra-epithelial mucous glands were identified, starting at the caudal region of the nasal vestibule and extended into the respiratory region. The olfactory region was lined with a pseudostratified epithelium that consisted of three different cell types: olfactory, support cells and basal cells. In conclusion, the current investigation presents new information concerning the histological, histochemical and ultrastructural features of the laughing dove's nasal cavity. Furthermore, the findings of this study may prove to be a valuable contribution to the avian histology and pathology literature.


Assuntos
Columbidae/anatomia & histologia , Cavidade Nasal/química , Cavidade Nasal/ultraestrutura , Animais , Feminino , Histocitoquímica/veterinária , Masculino , Microscopia Eletrônica de Varredura/veterinária , Cavidade Nasal/citologia , Bulbo Olfatório/química , Bulbo Olfatório/citologia , Bulbo Olfatório/ultraestrutura , Mucosa Respiratória/química , Mucosa Respiratória/citologia , Mucosa Respiratória/ultraestrutura
6.
Biofactors ; 43(3): 388-399, 2017 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-28139053

RESUMO

Ze339, an herbal extract from Petasites hybridus leaves is effective in treatment of allergic rhinitis by inhibition of a local production of IL-8 and eicosanoid LTB4 in allergen-challenged patients. However, the mechanism of action and anti-inflammatory potential in virally induced exacerbation of the upper airways is unknown. This study investigates the anti-inflammatory mechanisms of Ze339 on primary human nasal epithelial cells (HNECs) upon viral, bacterial and pro-inflammatory triggers. To investigate the influence of viral and bacterial infections on the airways, HNECs were stimulated with viral mimics, bacterial toll-like-receptor (TLR)-ligands or cytokines, in presence or absence of Ze339. The study uncovers Ze339 modulated changes in pro-inflammatory mediators and decreased neutrophil chemotaxis as well as a reduction of the nuclear translocation and phosphorylation of STAT molecules. Taken together, this study suggests that phyto drug Ze339 specifically targets STAT-signalling pathways in HNECs and has high potential as a broad anti-inflammatory drug that exceeds current indication. © 2016 BioFactors, 43(3):388-399, 2017.


Assuntos
Células Epiteliais/efeitos dos fármacos , Petasites/química , Extratos Vegetais/farmacologia , Fatores de Transcrição STAT/antagonistas & inibidores , Sesquiterpenos/farmacologia , Movimento Celular/efeitos dos fármacos , Quimiocinas/antagonistas & inibidores , Quimiocinas/biossíntese , Células Epiteliais/citologia , Células Epiteliais/metabolismo , Flagelina/antagonistas & inibidores , Flagelina/farmacologia , Regulação da Expressão Gênica , Humanos , Interferon gama/antagonistas & inibidores , Interferon gama/farmacologia , Interleucina-4/antagonistas & inibidores , Interleucina-4/farmacologia , Lipopeptídeos/antagonistas & inibidores , Lipopeptídeos/farmacologia , Cavidade Nasal/citologia , Cavidade Nasal/efeitos dos fármacos , Cavidade Nasal/metabolismo , Neutrófilos/efeitos dos fármacos , Folhas de Planta/química , Poli I-C/antagonistas & inibidores , Poli I-C/farmacologia , Cultura Primária de Células , Fatores de Transcrição STAT/genética , Fatores de Transcrição STAT/metabolismo , Transdução de Sinais
7.
PLoS One ; 11(12): e0168195, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-28002421

RESUMO

PURPOSE: Baicalin, a Chinese herbal medicine, has anti-fibrotic and anti-inflammatory effects. The aims of present study were to investigate the effects of baicalin on the myofibroblast differentiation, extracellular matrix production, migration, and collagen contraction of interleukin (IL)-1ß-stimulated nasal fibroblasts and to determine the molecular mechanism of baicalin in nasal fibroblasts. METHODS: Nasal fibroblasts were isolated from the inferior turbinate of patients. Baicalin was used to treat IL-1ß-stimulated nasal fibroblasts. To evaluate cytotoxicity, a 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide assay was used. The expression levels of α-smooth muscle actin (SMA), fibronectin, phospho-mitogen-activated protein kinase (p-MAPK), p-Akt, p-p50, p-p65, and p-IκBα were measured by western blotting, reverse transcription-polymerase chain reaction (RT-PCR),or immunofluorescence staining. Fibroblast migration was analyzed with scratch assays and transwell migration assays. Total collagen was evaluated with the Sircol collagen assay. Contractile activity was measured with a collagen gel contraction assay. RESULTS: Baicalin (0-50 µM) had no significant cytotoxic effects in nasal fibroblasts. The expression of α-SMA and fibronectin were significantly down-regulated in baicalin-treated nasal fibroblasts. Migration, collagen production, and contraction of IL-1ß-stimulated nasal fibroblasts were significantly inhibited by baicalin treatment. Baicalin also significantly down-regulated p-MAPK, p-Akt, p-p50, p-p65, and p-IκBα in IL-1ß-stimulated nasal fibroblasts. CONCLUSIONS: We showed that baicalin down-regulated myofibroblast differentiation, extracellular matrix production, migration, and collagen contraction via the MAPK and Akt/ NF-κB pathways in IL-1ß-stimulated nasal fibroblasts.


Assuntos
Regulação para Baixo/efeitos dos fármacos , Matriz Extracelular/metabolismo , Fibroblastos/efeitos dos fármacos , Flavonoides/farmacologia , Actinas/genética , Actinas/metabolismo , Adulto , Diferenciação Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Células Cultivadas , Colágeno/metabolismo , Feminino , Fibroblastos/citologia , Fibroblastos/metabolismo , Fibronectinas/metabolismo , Expressão Gênica/efeitos dos fármacos , Humanos , Interleucina-1beta/farmacologia , Masculino , Metaloproteinase 1 da Matriz/genética , Metaloproteinase 1 da Matriz/metabolismo , Proteínas Quinases Ativadas por Mitógeno/metabolismo , NF-kappa B/antagonistas & inibidores , NF-kappa B/metabolismo , Cavidade Nasal/citologia , Nitrilos/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Sulfonas/farmacologia
8.
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 30(10): 791-794;797, 2016 May 20.
Artigo em Chinês | MEDLINE | ID: mdl-29798055

RESUMO

Objective:To investigate the value of image-guided system in identifying the frontal recess cells.Method:We collected 30 cases that underwent image-guided frontal sinus surgery from November 2014 to December 2015. These frontal recess cells were devided into 2 groups based upon their locations in the frontal sinus ostium. Group A consists of the agger nasi cells, type Ⅰfrontal cells, type Ⅱ frontal cells and suprabullar cells; group B consist of type Ⅲ frontal cells, type Ⅳ frontal cells, frontal bullar cells, interfrontal sinus septal cells and supraorbital ethmoid cells. Visual analogue scale (VAS) was used to evaluate the degree of demand of image guide system on the location of frontal recess cells, and then analyzed the value of image guided system on the frontal recess cells.Result:In all 30 patients the imageguided frontal sinus surgery was successfully completed.The demand degree of image-guided system on frontal recess cells by VAS was slight for the agger nasi cells, type Ⅰfrontal cells, type Ⅱ frontal cells and suprabullar cells; the demand degree was general for the frontal bullar cells and interfrontal sinus septal cells; the demand degree was obvious for type Ⅲ frontal cells, type Ⅳ frontal cells and supraorbital ethmoid cells. Frontal recess cells of group B were more depended on image guided system than those of group A, and the difference was signicant(P <0.01).Conclusion:Imageguided system is valuable in distinguishing for type Ⅲ frontal cells,type Ⅳ frontal cells supraorbital ethmoid cells and interfrontal sinus septal cells.Furthermore,it is significantly helpful for accurate removal of these frontal recess cells in endoscopic frontal sinus surgery.


Assuntos
Seio Frontal/citologia , Cavidade Nasal/citologia , Endoscopia , Osso Etmoide , Seio Frontal/diagnóstico por imagem , Seio Frontal/cirurgia , Humanos , Cavidade Nasal/diagnóstico por imagem , Cavidade Nasal/cirurgia , Tomografia Computadorizada por Raios X
9.
Nucleic Acids Res ; 44(6): 2564-76, 2016 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-26615198

RESUMO

A mechanism by which control DNA elements regulate transcription over large linear genomic distances is by achieving close physical proximity with genes, and looping of the intervening chromatin paths. Alterations of such regulatory 'chromatin looping' systems are likely to play a critical role in human genetic disease at large. Here, we studied the spatial organization of a ≈790 kb locus encompassing the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Dysregulation of CFTR is responsible for cystic fibrosis, which is the most common lethal genetic disorder in Caucasian populations. CFTR is a relatively large gene of 189 kb with a rather complex tissue-specific and temporal expression profile. We used chromatin conformation at the CFTR locus to identify new DNA sequences that regulate its transcription. By comparing 5C chromatin interaction maps of the CFTR locus in expressing and non-expressing human primary cells, we identified several new contact points between the CFTR promoter and its surroundings, in addition to regions featuring previously described regulatory elements. We demonstrate that two of these novel interacting regions cooperatively increase CFTR expression, and suggest that the new enhancer elements located on either side of the gene are brought together through chromatin looping via CTCF.


Assuntos
Cromatina/química , Regulador de Condutância Transmembrana em Fibrose Cística/genética , Elementos Facilitadores Genéticos , Regiões Promotoras Genéticas , Cromatina/metabolismo , Fibrose Cística/genética , Fibrose Cística/metabolismo , Fibrose Cística/patologia , Regulador de Condutância Transmembrana em Fibrose Cística/química , Regulador de Condutância Transmembrana em Fibrose Cística/metabolismo , Células Epiteliais/citologia , Células Epiteliais/metabolismo , Fibroblastos/citologia , Fibroblastos/metabolismo , Loci Gênicos , Voluntários Saudáveis , Humanos , Cavidade Nasal/citologia , Cavidade Nasal/metabolismo , Cultura Primária de Células , Pele/citologia , Pele/patologia , Transcrição Genética
10.
Int Forum Allergy Rhinol ; 5(6): 551-6, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25821008

RESUMO

BACKGROUND: Chronic rhinosinusitis (CRS) is a cluster of disorders that result in sinonasal mucosal inflammation. Staphylococcus aureus (S. aureus) is associated with severe and recalcitrant CRS. The purpose of our study was to investigate the effect of S. aureus on respiratory epithelial barrier structure and function. METHODS: Conditioned media from S. aureus reference strains (American Type Culture Collection [ATCC] 13565, 14458, and 25923) was applied to air-liquid interface (ALI) cultures of primary human nasal epithelial cells (HNECs) and transepithelial electrical resistance (TEER) was measured to assess cell-to-cell integrity. Electron microscopy was used to gauge the ciliated area and tight junctions (TJs). Additionally, the expression of the TJ protein zona occludens-1 (ZO-1) was examined via immunofluorescence. Statistical analysis was performed using analysis of variance (ANOVA) with pairwise Bonferroni-adjusted t tests. RESULTS: Secreted products applied to ALI cultures from S. aureus strain 13565 caused a concentration-dependent decline in electrical impedance compared to controls and reference strains 14458 and 25923 (p < 0.001). Electron microscopy showed a distinct separation between adjacent cells apically, in the region of TJs. The ciliated area was not affected; however, ZO-1 expression became discontinuous in HNECs exposed to the 13565 strain's conditioned media. CONCLUSION: Conditioned media of the S. aureus strain 13565 damages the airway epithelium by disrupting the TJs between primary HNECs grown at an ALI. These findings suggest that strain-specific S. aureus-secreted product(s) compromise epithelial barrier function, which may constitute 1 of the roles played by S. aureus in the pathophysiology of recalcitrant CRS. Further research is required to uncover the relevant molecular mechanisms.


Assuntos
Junções Intercelulares/metabolismo , Mucosa Nasal/metabolismo , Mucosa Nasal/microbiologia , Staphylococcus aureus/fisiologia , Células Cultivadas , Meios de Cultivo Condicionados , Impedância Elétrica , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Microscopia Eletrônica , Cavidade Nasal/citologia , Mucosa Nasal/patologia , Junções Íntimas , Proteína da Zônula de Oclusão-1/metabolismo
11.
Biosci Biotechnol Biochem ; 79(6): 960-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25660503

RESUMO

An IF5 cDNA was isolated by expression cloning from a mouse oocyte cDNA library. It encoded a protein of 250 amino acids, and the region of it encoding amino acids 1-137 showed 86.8% alignment with the anti-proliferative domain of BTG/TOB family genes. This gene is also termed BTG4 or PC3B. Transiently expressed IF5/BTG4 induced alkaline phosphatase activity in human embryonic kidney (HEK293T) and 2T3 cells. IF5/BTG4 mRNA was detected by reverse transcription polymerase chain reaction in pharynx, larynx, trachea, oviduct, ovary, caput epididymis, and testis, but not in lung, intestine, or liver. Immunohistochemistry showed the IF5/BTG4 protein to be present in epithelial cells of the tongue, palate, pharynx, internal nose, and trachea. Both protein and mRNA levels of IF5/BTG4 were reduced by aging when comparing 4-week-old mice with 48-week-old mice. Our findings suggest that IF5/BTG4 may be an aging-related gene in epithelial cells.


Assuntos
Envelhecimento/metabolismo , Proteínas de Ciclo Celular/metabolismo , Boca/metabolismo , Cavidade Nasal/metabolismo , Traqueia/metabolismo , Envelhecimento/genética , Fosfatase Alcalina/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Proteínas de Ciclo Celular/química , Proteínas de Ciclo Celular/genética , Diferenciação Celular , Proliferação de Células , Clonagem Molecular , Regulação para Baixo , Células Epiteliais/citologia , Feminino , Células HEK293 , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Boca/citologia , Cavidade Nasal/citologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Traqueia/citologia
12.
Eur Rev Med Pharmacol Sci ; 19(3): 381-91, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25720707

RESUMO

OBJECTIVE: Nerve growth factor (NGF) is a neurotrophin which promote and regulate the survival of neurons in the peripheral nervous system. We aimed to evaluate the nasal NGF expressions of mast cells in healthy patients after stimulation with sterilized isotonic solution delivered at high pressure. PATIENTS AND METHODS: The first part of the study was made with 21 voluntary individuals. The middle third of the inferior turbinate epithelial cells on the right nostril was scraped using a sterile curette and indicated as (pre), than a spray of sterilized isotonic solution at high pressure on the left nostril was delivered and 25 minutes later a similar stimulation was delivered on the same nostril. The stimulation was made with a specific spray. The middle third of the inferior turbinate epithelial cells on the left nostril was scraped using a sterile curette and indicated as (post). RESULTS: Forced nasal stress induced by local delivery of high pressure physiological solution causes an increase in the number of mast cells and enhances level of NGF in the nasal fluid compared to the control subjects. So based on the first part of our study, since NGF is universally known as effective in protection and repairing of neural cells damage, we started the second part and gave a treatment on the same patients, to increase NGF levels with a six months daily therapy and observed the variations in Sensorineural Hearing Loss (SNHL) and tinnitus intensity from the beginning to the end of the therapy. All patients received sterilized isotonic solution at high pressure (pression emission level: PEL): 7 g/sec for 0.5 sec (emission time: ET) in both nostrils. 25 minutes later a similar stimulation was delivered twice a day. The control group (21 pts) received normal therapy with betahistine dihydrochloride 16 mg twice a day. CONCLUSIONS: Upon acuphenometry, there was a lower intensity of tinnitus and the improvement was signaled by the patients. Patients with SNHL treated with conventional therapy had a slight worsening, while the patients treated with our new therapy which increased NGF levels, showed improvement of hearing. This new therapy represents a new therapy of SNHL, tinnitus and hearing disorders.


Assuntos
Perda Auditiva Neurossensorial/terapia , Soluções Isotônicas/administração & dosagem , Mastócitos/metabolismo , Cavidade Nasal/metabolismo , Fator de Crescimento Neural/biossíntese , Zumbido/terapia , Adulto , Feminino , Perda Auditiva Neurossensorial/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade , Cavidade Nasal/citologia , Cavidade Nasal/efeitos dos fármacos , Seios Paranasais/citologia , Seios Paranasais/efeitos dos fármacos , Seios Paranasais/metabolismo , Pressão , Zumbido/diagnóstico
13.
Artigo em Chinês | MEDLINE | ID: mdl-24680338

RESUMO

OBJECTIVE: To study the human nasal ciliated epithelial cells at an air-liquid surface (ALI) so as to establish a reliable cell culture model for nasal mucociliary transport study. METHODS: The human nasal ciliated epithelial cells were cultured by low-temperature enzymatic digestion method at an air-liquid surface, the cell growth behavior was observed under the inverted phase-contrast microscope, the proliferation, confluence and differentiation of cultured cells were examined by scanning electron microscope and immunocytochemistry, the basal and stimulated ciliary beat frequency (CBF) of cultured epithelial cells were measured by using high-speed digital microscopic imaging system. Prism 4.0 software was used to analyze the data. RESULTS: (1) Under microscope, the cells on transwell membrane adhered well at 24 h and locked tightly to display with a cobblestone-like appearance; the monolayer cells got confluence to 80%-90% after one-week submersion culture, and thereafter exposed to air-liquid interface. (2) Under scanning electron microscope, the cilia and also the small microvilli could be observed to protrude from the cell's surface at ALI day 7; the ciliated cells differentiated well and distributed in cluster; goblet cells and nonciliated columnar cells distributed between ciliated cells. (3) Immunocytochemistry of ß-tubulin IV and zona occludens-1 showed a good confluence and differentiation of cilia in cultured epithelial cells at ALI day 14, and the percentage of ciliated epithelial cells was 50%-60 %. (4) The basal CBF of cultured epithelial cells was (8.42 ± 1.24), (8.71 ± 1.11), (9.17 ± 1.11), (8.89 ± 0.91), (8.99 ± 0.91) Hz at ALI day 7, 14 , 21, 28, 35, respectively, no significant difference was found among them(F = 1.451, P > 0.05). (5) At the concentration of 100 µmol/L ATP, an exogenous stimulating agent, significantly increased the CBF of cultured epithelial cells. CONCLUSIONS: Air-liquid interface cultured human nasal epithelial cells by enzymatic digestion method manifest with good confluence and differentiation status; the cells could maintain differentiated morphology and physiological function close to in vivo epithelium for a long term, therefore, it may serve as an ideal cell model for mucociliary transport study.


Assuntos
Técnicas de Cultura de Células , Células Epiteliais/citologia , Pólipos Nasais , Contagem de Células , Células Cultivadas , Humanos , Cavidade Nasal/citologia
14.
J Med Chem ; 57(6): 2813-9, 2014 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-24592914

RESUMO

A series of 3-oxo-C12-HSL, tetramic acid, and tetronic acid analogues were synthesized to gain insights into the structural requirements for quorum sensing inhibition in Staphylococcus aureus. Compounds active against agr were noncompetitive inhibitors of the autoinducing peptide (AIP) activated AgrC receptor, by altering the activation efficacy of the cognate AIP-1. They appeared to act as negative allosteric modulators and are exemplified by 3-tetradecanoyltetronic acid 17, which reduced nasal cell colonization and arthritis in a murine infection model.


Assuntos
Antibacterianos/farmacologia , Percepção de Quorum/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Animais , Antibacterianos/síntese química , Proteínas de Bactérias/antagonistas & inibidores , Proteínas de Bactérias/efeitos dos fármacos , Linhagem Celular , Furanos/síntese química , Furanos/farmacologia , Indicadores e Reagentes , Quelantes de Ferro/farmacologia , Camundongos , Testes de Sensibilidade Microbiana , Cavidade Nasal/citologia , Peptídeos Cíclicos/antagonistas & inibidores , Proteínas Quinases/efeitos dos fármacos , Pirrolidinonas/síntese química , Pirrolidinonas/farmacologia , Transdução de Sinais/efeitos dos fármacos , Bibliotecas de Moléculas Pequenas , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/microbiologia , Relação Estrutura-Atividade
15.
PLoS One ; 8(11): e78321, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24223790

RESUMO

INTRODUCTION: Little is known about how neonatal airway epithelial cell phenotype impacts on respiratory disease in later life. This study aimed to establish a methodology to culture and characterise neonatal nasal epithelial cells sampled from healthy, non-sedated infants within 48 hours of delivery. METHODS: Nasal epithelial cells were sampled by brushing both nostrils with an interdental brush, grown to confluence and sub-cultured. Cultured cells were characterised morphologically by light and electron microscopy and by immunocytochemistry. As an exemplar pro-inflammatory chemokine, IL-8 concentrations were measured in supernatants from unstimulated monolayers and after exposure to IL-1ß/TNF-α or house dust mite extract. RESULTS: Primary cultures were successfully established in 135 (91%) of 149 neonatal samples seeded, with 79% (n  =  117) successfully cultured to passage 3. The epithelial lineage of the cells was confirmed by morphological analysis and immunostaining. Constitutive IL-8 secretion was observed and was upregulated by IL-1ß/TNF-α or house dust mite extract in a dose dependent manner. CONCLUSION: We describe a safe, minimally invasive method of culturing nasal epithelial cells from neonates suitable for functional cell analysis offering an opportunity to study "naïve" cells that may prove useful in elucidating the role of the epithelium in the early origins of asthma and/or allergic rhinitis.


Assuntos
Misturas Complexas/farmacologia , Células Epiteliais/efeitos dos fármacos , Interleucina-1beta/farmacologia , Mucosa Respiratória/efeitos dos fármacos , Fator de Necrose Tumoral alfa/farmacologia , Animais , Proliferação de Células , Misturas Complexas/química , Relação Dose-Resposta Imunológica , Células Epiteliais/citologia , Células Epiteliais/imunologia , Feminino , Humanos , Recém-Nascido , Interleucina-8/biossíntese , Interleucina-8/metabolismo , Masculino , Cavidade Nasal/citologia , Cavidade Nasal/imunologia , Cultura Primária de Células , Pyroglyphidae/química , Mucosa Respiratória/citologia , Mucosa Respiratória/imunologia
16.
Biosci Biotechnol Biochem ; 77(10): 2154-6, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24096675

RESUMO

Solitary chemosensory cells in the non-neuronal epithelium of the anterior nasal cavity have bitter taste cell-like molecular characteristics and are involved in the detection of noxious substances. Here, we demonstrate that Pou2f3/Skn-1a, which is necessary for generation of sweet, umami, and bitter taste cells, is also necessary for the generation or differentiation of solitary chemosensory cells.


Assuntos
Diferenciação Celular , Células Epiteliais/citologia , Cavidade Nasal/citologia , Fatores de Transcrição de Octâmero/metabolismo , Animais , Células Epiteliais/metabolismo , Técnicas de Inativação de Genes , Camundongos , Fatores de Transcrição de Octâmero/deficiência , Fatores de Transcrição de Octâmero/genética
17.
Braz J Otorhinolaryngol ; 79(3): 285-92, 2013 May-Jun.
Artigo em Inglês, Português | MEDLINE | ID: mdl-23743742

RESUMO

UNLABELLED: The Agger nasi cell (ANC) and the frontal sinus ostium (FO) are important structures that can influence the anatomy and physiology of the frontal recess. The aim of this study was to evaluate the presence and size of ANC and the FO and correlate them according to gender, race and among themselves. METHOD: A prospective study with 40 patients who underwent CT of the paranasal sinuses with sagittal reconstruction. MEASUREMENTS: ANC (APAN) anteroposterior diameter, ANC (CCAN) craniocaudal diameter, ANC (LLAN) side-to-side diameter, anteroposterior diameter of the FO (APFO) and side-to-side diameter of the FO (LLFO). RESULTS: Twenty-two patients were male and 18 females, mean age 33.7 years. Most patients were white (45%), followed by browns (32.5%), blacks (20%) and asians (2.5%). The ANC was present in 98.7% of patients. There was statistical difference for APAN on females and LLAN on females and on the total sample. There were no differences for all measurements regarding gender, as well as the race. ANC and FO measurements showed positive correlation, but poor or very poor. CONCLUSION: The prevalence of ANC in our sample was high and did not show a statistically significant difference for most measurements. The correlation between measurements of ANC and the FO was poor or very poor.


Assuntos
Seio Frontal/diagnóstico por imagem , Cavidade Nasal/diagnóstico por imagem , Adulto , Grupos de Populações Continentais , Feminino , Seio Frontal/anatomia & histologia , Humanos , Masculino , Pessoa de Meia-Idade , Cavidade Nasal/citologia , Estudos Prospectivos , Valores de Referência , Tomografia Computadorizada por Raios X , Adulto Jovem
18.
Braz. j. otorhinolaryngol. (Impr.) ; 79(3): 285-292, maio-jun. 2013. ilus, tab
Artigo em Português | LILACS | ID: lil-675681

RESUMO

A célula do Agger nasi (CAN) e o óstio do seio frontal (OF) são estruturas importantes que podem influenciar a anatomofisiologia do recesso frontal. OBJETIVO: O objetivo deste trabalho foi avaliar a presença e as dimensões da CAN e do OF e correlacioná-las de acordo com o sexo, raça e entre si. MÉTODO: Estudo prospectivo com 40 pacientes submetidos à tomografia computadorizada de seios paranasais com reconstrução sagital. Foram realizadas as medidas: diâmetro ântero-posterior da CAN (AGAP), diâmetro crânio-caudal da CAN (AGCC), diâmetro látero-lateral da CAN (AGLL), diâmetro ântero-posterior do OF (OFAP) e diâmetro látero-lateral do OF (OFLL). RESULTADOS: Vinte e dois pacientes eram do sexo masculino e 18, do feminino; média de idade de 33,7 anos. A maioria dos pacientes era da raça branca (45%), seguidos pelos da raça parda (32,5%), da raça negra (20%) e da raça amarela. A CAN esteve presente em 98,7% das fossas nasais. Houve diferença estatística para AGAP no sexo feminino e AGLL no sexo feminino e na amostra total. Não houve diferenças para as medidas tanto quanto ao sexo como quanto à raça. As medidas da CAN e do OF apresentaram correlação, mas de maneira ruim ou péssima. CONCLUSÃO: A prevalência da CAN em nossa amostra foi alta e não houve diferença estatisticamente significante para a maioria das medidas realizadas. A correlação das medidas da CAN e do OF foi ruim ou péssima.


The Agger nasi cell (ANC) and the frontal sinus ostium (FO) are important structures that can influence the anatomy and physiology of the frontal recess. The aim of this study was to evaluate the presence and size of ANC and the FO and correlate them according to gender, race and among themselves. METHOD: A prospective study with 40 patients who underwent CT of the paranasal sinuses with sagittal reconstruction. Measurements: ANC (APAN) anteroposterior diameter, ANC (CCAN) craniocaudal diameter, ANC (LLAN) side-to-side diameter, anteroposterior diameter of the FO (APFO) and side-to-side diameter of the FO (LLFO). RESULTS: Twenty-two patients were male and 18 females, mean age 33.7 years. Most patients were white (45%), followed by browns (32.5%), blacks (20%) and asians (2.5%). The ANC was present in 98.7% of patients. There was statistical difference for APAN on females and LLAN on females and on the total sample. There were no differences for all measurements regarding gender, as well as the race. ANC and FO measurements showed positive correlation, but poor or very poor. CONCLUSION: The prevalence of ANC in our sample was high and did not show a statistically significant difference for most measurements. The correlation between measurements of ANC and the FO was poor or very poor.


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Seio Frontal , Cavidade Nasal , Grupos de Populações Continentais , Seio Frontal/anatomia & histologia , Cavidade Nasal/citologia , Estudos Prospectivos , Valores de Referência , Tomografia Computadorizada por Raios X
19.
Methods Mol Biol ; 945: 81-93, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23097102

RESUMO

The central cell type involved in the initial perception of odors and transduction of the sensory signal are the olfactory receptor neurons (ORNs) located in the olfactory neuroepithelium of the nasal cavities. The olfactory epithelium is a unique system similar to the neuroepithelium of the embryonic neural tube, in which new neurons are continually generated throughout adult life. Olfactory neurons are derived from precursor cells that lie adjacent to the basal lamina of the olfactory epithelium; these precursor cells divide several times and their progeny differentiate into mature sensory neurons throughout life. Thus, the human olfactory epithelium has the potential to be used as a tool to examine certain human disorders resulting from abnormal development of the nervous system. This chapter presents methods for primary culture of human ORNs, which have been used successfully by multiple investigators. The protocol provides a consistent, heterogeneous cell population, which demonstrates functional responses to odorant mixtures and exhibits a complex neuronal phenotype, encompassing receptors and signaling pathways pertinent to both olfaction and other aspects of CNS function. These cultured neural cells exhibit neurotransmitter pathways important in a number of neuropsychiatric disorders, and the ability to culture cells from living human subjects provides a tool for assessing cellular neuropathology at the individual patient level.


Assuntos
Técnicas de Cultura de Células/métodos , Cavidade Nasal/citologia , Células Neuroepiteliais/citologia , Cálcio/metabolismo , Separação Celular , Criopreservação , Humanos , Microscopia de Fluorescência , Células Neuroepiteliais/metabolismo
20.
Int J Pediatr Otorhinolaryngol ; 76(12): 1741-5, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22939589

RESUMO

OBJECTIVE: Non-allergic rhinitis is a heterogeneous disease whose etiology is largely unknown. Nasal cytology only allows us to recognize different non-allergic rhinitis forms on the basis of the prevalent inflammatory cell infiltrate: non-allergic rhinitis with eosinophils, with neutrophils, with mast-cells and with both eosinophils and mast-cells. The aim of this study is to define the incidence, clinical features and comorbidity of the different types of cell-mediated non-allergic rhinitis in a pediatric age group. METHODS: One hundred and fourteen non-allergic children with chronic nasal obstruction and associated symptoms (rhinorrhea, sneezing and nasal itchiness) were retrospectively selected. All patients had been submitted to a clinical history, pediatric evaluation, anterior rhinoscopy and fiberendoscopy, rhinomanometry and nasal cytology. RESULTS: Non-allergic rhinitis with neutrophils was present in 46 (40.4%) children, non-allergic rhinitis with eosinophils in 53 (46.5%), non-allergic rhinitis with mast-cells in 12 (10.5%) and non-allergic rhinitis with both eosinophils and mast-cells in 3 (2.6%). Nasal obstruction was prevalent in non-allergic rhinitis with eosinophils and in non-allergic rhinitis with mast-cells patients (P<0.001) whereas rhinorrea and sneezing only in the form with eosinophils (P<0.0001). Nasal itching on the other hand was prevalent in the form with mast-cells (P<0.0003). Non-allergic rhinitis with eosinophils group showed a higher probability of asthma (P<0.02) and respiratory sleep disorders (P<0.04). CONCLUSIONS: In the pediatric age group the most frequent forms of non-allergic rhinitis are those with eosinophils or with neutrophils. A diagnosis of non-allergic rhinitis with eosinophils in children presumes more severe symptoms and a higher incidence of pulmonary disease and roncopathy.


Assuntos
Eosinófilos/citologia , Mastócitos/citologia , Cavidade Nasal/citologia , Obstrução Nasal/etiologia , Rinite/diagnóstico , Adolescente , Distribuição por Idade , Análise de Variância , Criança , Pré-Escolar , Doença Crônica , Estudos de Coortes , Citodiagnóstico/métodos , Feminino , Humanos , Incidência , Itália/epidemiologia , Contagem de Leucócitos , Masculino , Líquido da Lavagem Nasal/citologia , Obstrução Nasal/epidemiologia , Obstrução Nasal/patologia , Prognóstico , Estudos Retrospectivos , Rinite/complicações , Rinite/epidemiologia , Rinomanometria , Medição de Risco , Distribuição por Sexo
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