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1.
J Bone Miner Metab ; 39(2): 212-223, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32772199

RESUMO

INTRODUCTION: Rickets, growth failure, and recurrent periapical abscesses with fistulae are main signs in patients with X-linked hypophosphatemic rickets (XLH). Prevalence of abscesses, pulp chamber features, biochemical findings, disease severity, and PHEX gene mutation were examined. MATERIALS AND METHODS: Pulp chambers size, shape, and morphology were assessed by orthopantomography in XLH patients (n = 24, age 5.8 ± 1.6 years) and in sex and age-matched healthy controls (n = 23, age 6.2 ± 1.4 years). XLH patients received conventional treatment (3.5 ± 1.9 years). Pulp chamber features were assessed in teeth of primary dentition and in the permanent left mandibular first molar and compared with those of controls. Rickets severity score was assessed at wrist, knee, and ankle. RESULTS: The mean pulp chamber area/tooth area ratio, mean pulp chamber height/pulp chamber width ratio, and prominence of pulp horns into the tooth crown in primary and secondary molars were significantly higher in patients than in controls and in patients suffered abscesses than in patients without abscesses. Sixteen patients (67%) had a history of abscesses; incisors were affected more than canines and molars. Severity of rickets and mean serum parathyroid hormone (PTH) levels were significantly higher, and mean serum 1,25-dihydroxyvitamin D [1,25(OH)2D] levels significantly lower in patients suffered abscesses than in patients without abscesses. PHEX gene mutations were not correlated with dental phenotype and disease severity. CONCLUSION: Enlarged pulp chambers with altered shape and morphology affected the majority of XLH patients predisposing to recurrent periapical abscesses with fistulae. Dental phenotype was associated with severity of rickets, high serum PTH, and low serum 1,25(OH)2D levels.


Assuntos
Abscesso/epidemiologia , Abscesso/genética , Cavidade Pulpar/patologia , Raquitismo Hipofosfatêmico Familiar/genética , Mutação/genética , Endopeptidase Neutra Reguladora de Fosfato PHEX/genética , Índice de Gravidade de Doença , Abscesso/patologia , Estudos de Casos e Controles , Criança , Feminino , Humanos , Masculino , Fenótipo , Prevalência
2.
Biomed Res Int ; 2020: 5465439, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33150176

RESUMO

Introduction: Enterococcus faecalis (E. faecalis) is the most important species in dentistry and plays a significant role in the etiology of persistent apical lesions after root canal treatment. Up to date, the intracanal application of 2% chlorhexidine for 7 days is the best way to eliminate E. faecalis. However, due to the ability of this bacterium to persist and survive in harsh environments, many studies have been directed towards finding an alternative strategy for prevention or eradication of it. This study was conducted to investigate the effect of bismuth nanoparticles on E. faecalis, as an etiologic factor in recurrent root canal infections. Methods: Forty patients, referred to Endodontic Ward of Shiraz University of Medical Science for endodontic pretreatment, provided root canal samples. First, all samples were transferred in Enterococcosel broth and incubated. Then, samples which showed growth were plated on blood agar plates and incubated for further PCR procedure. Nanoparticle powder was dissolved in high-purity water, and the final concentration of bismuth nanoparticles (BiNPs) was measured by the spectrophotometer. Minimum inhibitory concentration (MIC) of BiNPs against E. faecalis was determined by microbroth dilution method according to methods for antimicrobial susceptibility tests. Also, bactericidal assays were conducted in Mueller-Hinton broth medium and reported as the concentration of BiNPs that reduced the viable bacterial count by 99.9%. Results: Of all samples, 77.5% revealed the presence of E. faecalis by PCR. Also, E. faecalis growth inhibition was observed at concentrations ranging from 0.625 µg/ml to 20 µg/ml (geometric mean: 2.337 µg/ml), and the MBC values were between 1.25 µg/ml and 40 µg/ml (geometric mean: 4.781 µg/ml), which in comparison with chlorhexidine, these values were about one-eighth of chlorhexidine. Conclusion: The experimental data suggest that bismuth nanoparticles could be an interesting alternative to combat E. faecalis, which, in view of the advantages mentioned for bismuth nanoparticle like inhibiting Streptococcus mutans biofilm formation and higher antibacterial activity compared to chlorhexidine, can be suggested to be used in different fields of dentistry.


Assuntos
Antibacterianos/farmacologia , Bismuto/farmacologia , Enterococcus faecalis/efeitos dos fármacos , Nanopartículas Metálicas/química , Tratamento do Canal Radicular/métodos , Adolescente , Adulto , Clorexidina/farmacologia , Cavidade Pulpar/microbiologia , Cavidade Pulpar/patologia , Enterococcus faecalis/crescimento & desenvolvimento , Enterococcus faecalis/patogenicidade , Feminino , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Infecções por Bactérias Gram-Positivas/microbiologia , Infecções por Bactérias Gram-Positivas/patologia , Humanos , Masculino , Nanopartículas Metálicas/ultraestrutura , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade
3.
Biomed Res Int ; 2020: 7912638, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33062699

RESUMO

This study evaluated the long-term clinical outcomes of single-visit root canal treatments with apical enlargement on patients with necrotic pulp tissue retrospectively. A total of 137 teeth with necrotic pulp tissue which underwent single-visit root canal treatments were included. The root canals were shaped up until the apical constriction, which was determined by an apex locator. The outcomes were evaluated by two independent and calibrated endodontists clinically and radiographically. Teeth were dichotomized into healed (PAI ≤ 2, no signs or symptoms) and nonhealed (PAI > 2, with/without signs or symptoms) groups. Each patients' preoperative PAI and lesion size were recorded to evaluate the preoperative periapical status as well as several other prognostic factors. Statistical analyses were performed (p = 0.05) on ninety teeth. The mean observation time was 60 months. Out of ninety teeth, 87 (96.7%) were healed and 3 (3.3%) were nonhealed. No correlations were found between the prognostic factors and the outcomes (p > 0.05). Cohen's kappa and Gwet's agreement coefficient scores between the preoperative PAI scores and preoperative lesion sizes showed good agreements, with values of 0.834 and 0.898, respectively. Apical enlargement is a viable treatment option for single-visit root canal treatments.


Assuntos
Cavidade Pulpar , Necrose da Polpa Dentária , Tratamento do Canal Radicular/métodos , Ápice Dentário , Adulto , Cavidade Pulpar/diagnóstico por imagem , Cavidade Pulpar/patologia , Cavidade Pulpar/cirurgia , Necrose da Polpa Dentária/diagnóstico por imagem , Necrose da Polpa Dentária/patologia , Necrose da Polpa Dentária/cirurgia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia Dentária , Estudos Retrospectivos , Tratamento do Canal Radicular/efeitos adversos , Tratamento do Canal Radicular/estatística & dados numéricos , Ápice Dentário/diagnóstico por imagem , Ápice Dentário/patologia , Ápice Dentário/cirurgia , Resultado do Tratamento
4.
Biomed Res Int ; 2020: 7890127, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33102594

RESUMO

An accurate estimation of the working canal length is essential for successful root canal treatment. This study is aimed at investigating the diagnostic accuracy of root canal length estimation on cone-beam computed tomography (CBCT) scans and digital paralleling radiographs (PAs), using the real canal length as a gold standard, and at evaluating the influence of canal curvature on this estimation. Sixty extracted human premolar teeth were selected for this study. Root canal length measurement was performed on CBCT scans (NewTom, Giano, Verona, Italy) and digital paralleling radiography (EzRay Air W; Vatech, Korea). The real working length was established by subtracting 0.5 mm from the actual canal length. No significant difference was found between CBCT and digital paralleling radiography. There was a tendency for underestimation of the root canal length measured on the CBCT images in 52 (86.7%) of the examined teeth and overestimation in 5 teeth (8.3%). All the digital radiographs slightly overestimated the real canal length. The analysis revealed a strong correlation between the estimation from moderate to severe curvature for digital radiography and CBCT images. Preoperative working length estimation can be made closest to its real clinical canal length on the standardized paralleling technique, using a long (16-inch) target-receptor distance.


Assuntos
Tomografia Computadorizada de Feixe Cônico/métodos , Cavidade Pulpar/diagnóstico por imagem , Cavidade Pulpar/patologia , Radiografia Dentária Digital/métodos , Dente Pré-Molar/diagnóstico por imagem , Dente Pré-Molar/patologia , Modelos Dentários , Humanos , Técnicas In Vitro , Reprodutibilidade dos Testes , Preparo de Canal Radicular/métodos , Tratamento do Canal Radicular/métodos
5.
Niger J Clin Pract ; 23(9): 1237-1242, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32913162

RESUMO

Aims: We evaluated and compared EndoActivator, CanalBrush, and passive ultrasonic irrigation (PUI) in the removal of calcium hydroxide and calcium hydroxide with iodoform and p-chlorophenol paste (Calcipast Forte) from artificial standardized grooves in the apical third of root canals. Materials and Methods: A total of 34 mandibular premolars were prepared and then split longitudinally. A standardized groove was prepared in the apical part of both segments. The grooves were filled with either calcium hydroxide or Calcipast Forte, and the segments were reassembled. CanalBrush, EndoActivator, or PUI were used. The amount of remaining medicament was evaluated using a four-grade scoring system. Results: None of the irrigation methods could completely remove the pastes from the grooves. More Calcipast Forte paste was detected compared with calcium hydroxide (P < 0.01). PUI was the least effective method in removing Calcipast Forte. Conclusions: It was more difficult to remove Calcipast Forte than a water-based calcium hydroxide paste.


Assuntos
Hidróxido de Cálcio/química , Clorofenóis , Cavidade Pulpar/patologia , Hidrocarbonetos Iodados , Materiais Restauradores do Canal Radicular/química , Irrigantes do Canal Radicular/administração & dosagem , Terapia por Ultrassom/métodos , Humanos , Irrigantes do Canal Radicular/química , Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Irrigação Terapêutica/métodos , Terapia por Ultrassom/instrumentação , Ultrassom , Água
6.
PLoS One ; 15(9): e0239660, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32991601

RESUMO

The purpose of this study was to develop a high-frequency wave therapy model in rats and to investigate the influence of high-frequency waves on root canal treatment, which may provide a novel strategy for treating apical periodontitis. Root canal treatments with and without high-frequency wave irradiation were performed on the mandibular first molars of 10-week-old male Wistar rats. The mesial roots were evaluated radiologically, bacteriologically, and immunohistochemically. At 3 weeks after root canal treatment, lesion volume had decreased significantly more in the irradiated group than in the non-irradiated group, indicating successful development of the high-frequency therapy model. The use of high-frequency waves provided no additional bactericidal effect after root canal treatment. However, high-frequency wave irradiation was found to promote healing of periapical lesions on the host side through increased expression of fibroblast growth factor 2 and transforming growth factor-ß1 and could therefore be useful as an adjuvant nonsurgical treatment for apical periodontitis.


Assuntos
Cavidade Pulpar/patologia , Periodontite Periapical/terapia , Ondas de Rádio , Tratamento do Canal Radicular/métodos , Animais , Bactérias/genética , Bactérias/isolamento & purificação , DNA Bacteriano/isolamento & purificação , DNA Bacteriano/metabolismo , Cavidade Pulpar/diagnóstico por imagem , Fator 2 de Crescimento de Fibroblastos/metabolismo , Imageamento Tridimensional , Interleucina-1beta/metabolismo , Masculino , Dente Molar/patologia , Periodontite Periapical/microbiologia , Periodontite Periapical/patologia , Ratos , Ratos Wistar , Fator de Crescimento Transformador beta1/metabolismo , Microtomografia por Raio-X
7.
J Appl Oral Sci ; 28: e20190699, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32401938

RESUMO

Purpose To evaluate the kinetics of apical periodontitis development in vivo , induced either by contamination of the root canals by microorganisms from the oral cavity or by inoculation of bacterial lipopolysaccharide (LPS) and the regulation of major enzymes and receptors involved in the arachidonic acid metabolism. Methodology Apical periodontitis was induced in C57BL6 mice (n=96), by root canal exposure to oral cavity (n=48 teeth) or inoculation of LPS (10 µL of a suspension of 0.1 µg/µL) from E. coli into the root canals (n= 48 teeth). Healthy teeth were used as control (n=48 teeth). After 7, 14, 21 and 28 days the animals were euthanized and tissues removed for histopathological and qRT-PCR analyses. Histological analysis data were analyzed using two-way ANOVA followed by Sidak's test, and qRT-PCR data using two-way ANOVA followed by Tukey's test (α=0.05). Results Contamination by microorganisms led to the development of apical periodontitis, characterized by the recruitment of inflammatory cells and bone tissue resorption, whereas inoculation of LPS induced inflammatory cells recruitment without bone resorption. Both stimuli induced mRNA expression for cyclooxygenase-2 and 5-lipoxygenase enzymes. Expression of prostaglandin E 2 and leukotriene B 4 cell surface receptors were more stimulated by LPS. Regarding nuclear peroxisome proliferator-activated receptors (PPAR), oral contamination induced the synthesis of mRNA for PPARδ, differently from inoculation of LPS, that induced PPARα and PPARγ expression. Conclusions Contamination of the root canals by microorganisms from oral cavity induced the development of apical periodontitis differently than by inoculation with LPS, characterized by less bone loss than the first model. Regardless of the model used, it was found a local increase in the synthesis of mRNA for the enzymes 5-lipoxygenase and cyclooxygenase-2 of the arachidonic acid metabolism, as well as in the surface and nuclear receptors for the lipid mediators prostaglandin E2 and leukotriene B4.


Assuntos
Cavidade Pulpar/microbiologia , Dinoprostona/metabolismo , Leucotrieno B4/metabolismo , Lipopolissacarídeos/metabolismo , Periodontite Periapical/microbiologia , Animais , Araquidonato 5-Lipoxigenase/análise , Araquidonato 5-Lipoxigenase/metabolismo , Reabsorção Óssea/metabolismo , Reabsorção Óssea/microbiologia , Ciclo-Oxigenase 2/análise , Ciclo-Oxigenase 2/metabolismo , Cavidade Pulpar/metabolismo , Cavidade Pulpar/patologia , Dinoprostona/análise , Expressão Gênica , Leucotrieno B4/análise , Masculino , Camundongos Endogâmicos C57BL , Periodontite Periapical/metabolismo , Periodontite Periapical/patologia , RNA Mensageiro/análise , RNA Mensageiro/metabolismo , Distribuição Aleatória , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Tempo
8.
BMC Oral Health ; 20(1): 111, 2020 04 16.
Artigo em Inglês | MEDLINE | ID: mdl-32299409

RESUMO

BACKGROUND: Evaluate the fracture resistance of endodontically treated teeth after cervical preflaring and root canal preparation and to assess the volume of the root canal and the amount of remaining root dentin before and after cervical preflaring. METHODS: Forty-four mandibular incisors were selected using micro-CT scanning and distributed into 4 groups (n = 11) according to the instrument used for cervical preflaring: control group - no cervical preflaring; Gates Glidden - burs size #2 and #3; WXN - 25.07 Navigator instrument; and Easy - 25.08 ProDesign S instrument. Coronal opening was performed, and the canals were prepared with Wave One Gold Primary and filled with an epoxy-resin based sealer and gutta-percha cones. Micro-CT scans were performed before and after root canal instrumentation. All images were reconstructed and assessed for the thickness of mesial and distal root dentin at 3 mm and 5 mm from the cement -enamel junction and for the volume of cervical portion of the canal after preparation. Fracture resistance test was performed applying compressive loads at a crosshead speed of 0.5 mm/min, applied on the palatal aspect of specimens at 135° along the long axis of the tooth. The data were analyzed using ANOVA and Tukey's test (P = .05). RESULTS: Cervical preflaring and canal preparation reduced the dentin thickness (P < .05) and increased the canal volume (P < .05) in all groups at 3 mm an 5 mm. Cervical preflaring with Gates Gliden burs reduced the fracture resistance of endodontically treated teeth (P < .05). CONCLUSIONS: All instruments reduced the dentin thickness and increased the canal volume in the cervical at 3 mm and 5 mm. Gates Glidden reduced fracture resistance of mandibular incisors submitted to cervical preflaring, whereas NiTi instruments did not. CLINICAL RELEVANCE: Cervical preflaring assumes particular importance previously to the root canal preparation because it minimizes the occurrence of operative accidents, and permits more accurate determination of working length and the apical diameter.


Assuntos
Cavidade Pulpar/patologia , Guta-Percha/uso terapêutico , Materiais Restauradores do Canal Radicular/uso terapêutico , Preparo de Canal Radicular/instrumentação , Dente não Vital/fisiopatologia , Resinas Epóxi/uso terapêutico , Humanos , Preparo de Canal Radicular/métodos , Fraturas dos Dentes , Dente não Vital/diagnóstico por imagem
10.
BMC Oral Health ; 20(1): 29, 2020 01 30.
Artigo em Inglês | MEDLINE | ID: mdl-32000767

RESUMO

BACKGROUND: To report a case of type III dens invaginatus associated with peri-invagination periodontitis in an immature permanent mandibular central incisor with open apex, in which only the invagination area was treated and vitality was preserved. CASE PRESENTATION: A 9-year-old boy was referred complaining of pain in the mandibular left central incisor. After radiographic examination, an invagination into the pulp chamber of the tooth associated with periapical radiolucency was detected. Endodontic access was performed and the orifice was identified under a dental operating microscope. The invagination area was chemo-mechanically cleaned. After 1 week, the invagination was obturated with mineral trioxide aggregate. During the 2-year follow up period, the tooth was asymptomatic. Radiographic examination revealed significant progression of periapical healing and root development in the main root canal of the tooth. CONCLUSION: Non-surgical root canal treatment of the invagination may preserve pulp vitality, and continuous root development of the tooth.


Assuntos
Dens in Dente/terapia , Incisivo/anormalidades , Periodontite Periapical/terapia , Criança , Cavidade Pulpar/patologia , Necrose da Polpa Dentária/terapia , Humanos , Incisivo/diagnóstico por imagem , Masculino , Radiografia Interproximal , Reprodutibilidade dos Testes , Tratamento do Canal Radicular , Coroa do Dente/anormalidades , Coroa do Dente/diagnóstico por imagem , Raiz Dentária/anormalidades , Raiz Dentária/diagnóstico por imagem
11.
Niger J Clin Pract ; 23(2): 212-218, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32031096

RESUMO

Objectives: This single-blind, randomized clinical trial (RCT) aimed to compare the duration, intensity, and incidence of postoperative pain after foraminal enlargement (FE) with continuous rotary systems and reciprocating instruments. Materials and Methods: Sixty qualified patients were randomly divided into the following two groups: the ProTaper Next group and the WaveOne group. Participants were selected from patients who had both asymptomatic necrosis and asymptomatic apical periodontitis with a single root canal. Endodontic treatment was performed in one visit, and the patients were asked to record their pain severity and analgesic consumption during a 7-day follow-up period using a visual analog scale (VAS). The data were analyzed using the Mann-Whitney U-test and Chi-square test (P < 0.05). Results: A significant difference was observed between the two groups during the first two days of follow-up (P < 0.05). Pain experience was higher in FEs that had been created by reciprocating instruments than by continuous rotary systems. There were no significant differences in VAS pain scores over the other days (P > 0.05). None of the patients had severe postoperative pain during the follow-up period. No significant differences were observed in the prevalence of analgesic consumption between either group (P > 0.05). Conclusions: This RCT indicates that in the 2-day follow-up period after endodontic treatment, FEs created by reciprocated instruments associated more postoperative pain than continuous rotary systems.


Assuntos
Cavidade Pulpar/cirurgia , Necrose da Polpa Dentária/terapia , Dente Molar/cirurgia , Dor Pós-Operatória/epidemiologia , Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Tratamento do Canal Radicular/instrumentação , Adulto , Analgésicos/uso terapêutico , Cavidade Pulpar/patologia , Feminino , Humanos , Incidência , Masculino , Dor Pós-Operatória/etiologia , Irrigantes do Canal Radicular/uso terapêutico , Método Simples-Cego , Resultado do Tratamento , Escala Visual Analógica
12.
Acta Odontol Scand ; 78(5): 332-336, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31986947

RESUMO

Objective: The aim of this study was to compare apical debris extrusion when neodymium-doped yttrium aluminium garnet (Nd:YAG) lasers, erbium-doped yttrium aluminium garnet (Er:YAG) or photon-induced photoacoustic streaming (PIPS) are used for irrigation activation.Materials and methods: A total of 60 extracted human mandibular premolar teeth have similar dimensions were included and the samples were split into four groups according to the irrigation technique (n = 15): conventional needle irrigation, PIPS, Er:YAG and Nd:YAG. ProTaper Universal system up to F4 was used for root canal instrumentation. Bidistilled water was used as an irrigation solution during instrumentation and irrigation activation. Apically extruded debris was collected into preweighed Eppendorf tubes during instrumentation and irrigation activation procedures. The tubes were then kept in an incubator at 70 °C for 5 days. The initial weight of the tube was subtracted from the final weight and the result was recorded as the weight of dry extruded debris. The data were evaluated statistically using a one-way ANOVA test followed by least significant difference post hoc test (p < .05).Results: Conventional needle irrigation caused significantly less debris extrusion than laser-assisted irrigation activation groups (p < .05). Laser-assisted irrigation activation groups caused statistically similar debris extrusion (p > .05).Conclusion: Laser-assisted irrigation activation techniques caused more debris extrusion when compared to conventional needle irrigation.


Assuntos
Cavidade Pulpar/patologia , Lasers de Estado Sólido , Irrigação Terapêutica/métodos , Ápice Dentário/efeitos dos fármacos , Ápice Dentário/efeitos da radiação , Dente Pré-Molar , Humanos , Agulhas , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular , Irrigação Terapêutica/instrumentação , Ápice Dentário/patologia
13.
Pan Afr Med J ; 37: 254, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33598069

RESUMO

A case of pulp canal obliteration (PCO) two years after the completion of orthodontic treatment is presented. Post orthodontic treatment PCO is a rare finding. A 23 years old female presented with the discoloration of clinical crown of maxillary right central incisor. Radiographic examination revealed the calcified canal and diffused periapical radiolucency. Vitality tests were negative. A decision was made to do root canal treatment followed by the walking bleaching. After the successful completion of the root canal treatment the internal bleaching was performed. The discolored tooth showed significant improvement in color. Internal bleaching is a viable conservative treatment for improving esthetics in single non vital discolored tooth.


Assuntos
Cavidade Pulpar/patologia , Tratamento do Canal Radicular/métodos , Clareamento Dental , Descoloração de Dente/terapia , Feminino , Humanos , Incisivo , Ortodontia , Descoloração de Dente/etiologia , Adulto Jovem
14.
J Med Life ; 13(4): 635-640, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33456616

RESUMO

Microscopic studies performed on extracted human teeth after their preparation in advance is helpful in a relatively good reestablishment of the treatment steps that have been applied to these teeth, as well as an evaluation of the quality of such treatments. Therefore, we have used stereo- and optical microscopy, highlighting aspects of external morphology, as well as root canal space of an extracted mandibular second premolar, subjected to prosthetic and endodontic treatment. In order to verify some technical errors that might occur during the endodontic and restorative treatment, we tried to appreciate the quality of the root canal filling and cervical defect and access cavity restoration of an extracted premolar #45.Without having the data from clinical records, we concluded that the method used for root canal filling was the warm lateral condensation technic; we also appreciated the quality of the fusion of the gutta-percha cones used, so the introduction of heated spreaders only in the central part of the bunch of cones makes it possible to clearly detect the boundaries between these cones towards the outside of the filling.


Assuntos
Dente Pré-Molar/patologia , Doença Iatrogênica , Mandíbula/patologia , Microscopia , Cavidade Pulpar/patologia , Feminino , Humanos , Pessoa de Meia-Idade , Materiais Restauradores do Canal Radicular/uso terapêutico , Tratamento do Canal Radicular
15.
J. appl. oral sci ; 28: e20190699, 2020. graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1134770

RESUMO

Abstract Purpose To evaluate the kinetics of apical periodontitis development in vivo , induced either by contamination of the root canals by microorganisms from the oral cavity or by inoculation of bacterial lipopolysaccharide (LPS) and the regulation of major enzymes and receptors involved in the arachidonic acid metabolism. Methodology Apical periodontitis was induced in C57BL6 mice (n=96), by root canal exposure to oral cavity (n=48 teeth) or inoculation of LPS (10 µL of a suspension of 0.1 µg/µL) from E. coli into the root canals (n= 48 teeth). Healthy teeth were used as control (n=48 teeth). After 7, 14, 21 and 28 days the animals were euthanized and tissues removed for histopathological and qRT-PCR analyses. Histological analysis data were analyzed using two-way ANOVA followed by Sidak's test, and qRT-PCR data using two-way ANOVA followed by Tukey's test (α=0.05). Results Contamination by microorganisms led to the development of apical periodontitis, characterized by the recruitment of inflammatory cells and bone tissue resorption, whereas inoculation of LPS induced inflammatory cells recruitment without bone resorption. Both stimuli induced mRNA expression for cyclooxygenase-2 and 5-lipoxygenase enzymes. Expression of prostaglandin E 2 and leukotriene B 4 cell surface receptors were more stimulated by LPS. Regarding nuclear peroxisome proliferator-activated receptors (PPAR), oral contamination induced the synthesis of mRNA for PPARδ, differently from inoculation of LPS, that induced PPARα and PPARγ expression. Conclusions Contamination of the root canals by microorganisms from oral cavity induced the development of apical periodontitis differently than by inoculation with LPS, characterized by less bone loss than the first model. Regardless of the model used, it was found a local increase in the synthesis of mRNA for the enzymes 5-lipoxygenase and cyclooxygenase-2 of the arachidonic acid metabolism, as well as in the surface and nuclear receptors for the lipid mediators prostaglandin E2 and leukotriene B4.


Assuntos
Animais , Masculino , Periodontite Periapical/microbiologia , Dinoprostona/metabolismo , Lipopolissacarídeos/metabolismo , Leucotrieno B4/metabolismo , Cavidade Pulpar/microbiologia , Periodontite Periapical/metabolismo , Periodontite Periapical/patologia , Fatores de Tempo , Reabsorção Óssea/metabolismo , Reabsorção Óssea/microbiologia , Araquidonato 5-Lipoxigenase/análise , Araquidonato 5-Lipoxigenase/metabolismo , RNA Mensageiro/análise , RNA Mensageiro/metabolismo , Dinoprostona/análise , Distribuição Aleatória , Expressão Gênica , Leucotrieno B4/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Cavidade Pulpar/metabolismo , Cavidade Pulpar/patologia , Ciclo-Oxigenase 2/análise , Ciclo-Oxigenase 2/metabolismo , Camundongos Endogâmicos C57BL
16.
Santiago; MINSAL; 2020. 13 p.
Não convencional em Espanhol | BIGG - guias GRADE | ID: biblio-1177249

RESUMO

Generar recomendaciones basadas en la mejor evidencia disponible acerca del manejo de personas con diagnóstico de patologías pulpares o periapicales en dentición permanente. Personas con diagnóstico de patología pulpar o periapical en diente permanente que reciben atención en el nivel primario de salud en el sector público y privado de salud.


Assuntos
Humanos , Doenças Periapicais/prevenção & controle , Dentição Permanente , Cavidade Pulpar/patologia
17.
Medicine (Baltimore) ; 98(40): e17356, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31577730

RESUMO

Molar-incisor malformation (MIM) is a recently defined dental abnormality of molar root and incisors, and introduced as one of the causes of periapical and periodontal abscess. The purpose of this study was to investigate the clinical and radiological features of MIM, with special emphasis on various medical history. A total of 38 patients with MIM were included in this study. Radiographic features and clinical data, including medical history, chief complaint, associated complications, treatment, and prognosis, were retrospectively investigated. On radiographs, the affected molars showed short, slender, underdeveloped roots and constricted pulp chambers. All affected incisors and canines exhibited dilacerated short roots, wedge-shaped defect on the cervical part of the crown. Complications included periodontal bone loss (52.6%), endodontic lesion (50.0%), and endodontic-periodontal lesion (28.9%). The medical histories of the patients with MIM indicate that almost all (94.7%) were hospitalized due to problems during the neonatal period. MIM may cause various dental problems, such as periapical and periodontal abscess and early loss of the affected teeth. The early diagnosis of MIM on radiographs and appropriate treatment will contribute to a favorable prognosis, especially for young and adolescent patients.


Assuntos
Incisivo/diagnóstico por imagem , Incisivo/patologia , Dente Molar/diagnóstico por imagem , Dente Molar/patologia , Anormalidades Dentárias/diagnóstico por imagem , Anormalidades Dentárias/patologia , Adolescente , Perda do Osso Alveolar/etiologia , Criança , Pré-Escolar , Cavidade Pulpar/diagnóstico por imagem , Cavidade Pulpar/patologia , Feminino , Humanos , Masculino , Radiografia Dentária , Estudos Retrospectivos , Anormalidades Dentárias/complicações , Anormalidades Dentárias/diagnóstico , Raiz Dentária/diagnóstico por imagem , Raiz Dentária/patologia , Adulto Jovem
18.
Br J Oral Maxillofac Surg ; 57(8): 793-795, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31375300

RESUMO

We present a 16-year-old boy with a history of ankylosis of the temporomandibular joint (TMJ) who had been treated with a costochondral graft and mandibular distraction. The distraction seems to have caused pulp canal obliteration of the lower right second premolar and lower right first molar on radiographic examination. To our knowledge this is the only reported instance of such damage related to mandibular distraction. We aim to highlight the risks of this complication and the importance of discussing it with patients as part of the process of informed consent.


Assuntos
Anquilose , Cavidade Pulpar/patologia , Osteogênese por Distração , Articulação Temporomandibular , Anquilose Dental , Adolescente , Anquilose/cirurgia , Humanos , Masculino , Mandíbula , Osteogênese por Distração/efeitos adversos , Articulação Temporomandibular/cirurgia
19.
Braz Oral Res ; 33: e084, 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31460610

RESUMO

This study aimed to evaluate the role of photobiomodulation (PBM) in apexification and apexogenesis of necrotic rat molars with an open apex. Rat molars were exposed to the oral environment for 3 weeks. Canals were rinsed with 2.5% NaOCl and 17% EDTA, filled with antibiotic paste and sealed. After 7 days, canals were rinsed and divided into six groups (n=6): mineral trioxide aggregate (MTA); blood clot (BC); human dental pulp stem cells (hDPSC); MTA+PBM; BC+PBM; and hDPSC+PBM. In hDPSC groups, a 1% agarose gel scaffold was used. Two groups were not exposed: healthy tooth+PBM (n = 6), healthy tooth (n = 3); and one was exposed throughout the experiment: necrotic tooth (n = 3). In PBM groups, irradiation was performed with aluminum gallium indium phosphide (InGaAlP) diode laser for 30 days within 24-h intervals. After that, the specimens were processed for histological and immunohistochemical analyses. Necrotic tooth showed greater neutrophil infiltrate (p < 0.05). Necrotic tooth, healthy tooth, and healthy tooth+PBM groups showed absence of a thin layer of fibrous condensation in the periapical area. All the other groups stimulated the formation of a thicker layer of fibers (p < 0.05). All groups formed more mineralized tissue than necrotic tooth (p < 0.05). PBM associated with MTA, BC, or hDPSC formed more mineralized tissue (p < 0.05). MTA+PBM induced apexification (p < 0.05). Rabbit polyclonal anti-bone sialoprotein (BSP) antibody confirmed the histological findings of mineralized tissue formation, and hDPSC groups exhibited higher percentage of BSP-positive cells. It can be concluded that PBM improved apexification and favored apexogenesis in necrotic rat molars with an open apex.


Assuntos
Apexificação/métodos , Cavidade Pulpar/efeitos da radiação , Necrose da Polpa Dentária/radioterapia , Lasers Semicondutores/uso terapêutico , Terapia com Luz de Baixa Intensidade/métodos , Ápice Dentário/efeitos da radiação , Doenças Dentárias/radioterapia , Compostos de Alumínio/uso terapêutico , Animais , Compostos de Cálcio/uso terapêutico , Polpa Dentária/citologia , Cavidade Pulpar/patologia , Necrose da Polpa Dentária/patologia , Combinação de Medicamentos , Imuno-Histoquímica , Sialoproteína de Ligação à Integrina/análise , Óxidos/uso terapêutico , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Silicatos/uso terapêutico , Células-Tronco , Ápice Dentário/patologia , Doenças Dentárias/patologia , Resultado do Tratamento
20.
Pathol Int ; 69(6): 360-365, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31215141

RESUMO

A fungal ball of a maxillary sinus sometimes includes dental treatment-related foreign material because the sinus is close to the root of the upper teeth. We present a case of right maxillary sinus fungal ball with a gutta-percha point, a dental root canal filler. X-ray analysis of the foreign material in the paraffin section of the fungal ball successfully detected zinc, sulfur, and barium, all of which were constituents of the gutta-percha point. The gutta-percha point might have facilitated the formation of the fungal ball through disruption of the sinus-clearing mechanism. Another interesting histological feature of the fungal ball was the finding of calcium oxalate crystals and non-hyphal fungal elements such as cleistothecia, Hülle cells, and conidial heads. This is the first report of such a combination being found in a specimen of human fungal disease. Although fungal culture was not available in the present case, molecular analysis of the formalin-fixed paraffin-embedded tissue of the fungal ball succeeded in revealing only DNA sequences of Aspergillus nidulans and some other environmental Aspergillus spp.


Assuntos
Cavidade Pulpar/patologia , Maxila/patologia , Seio Maxilar/microbiologia , Seio Maxilar/patologia , Aspergillus nidulans/patogenicidade , Cavidade Pulpar/microbiologia , Proteínas Fúngicas/metabolismo , Guta-Percha , Humanos , Masculino , Maxila/microbiologia , Pessoa de Meia-Idade , Micoses/diagnóstico , Micoses/patologia , Dente/microbiologia , Dente/patologia
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