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1.
Rev Col Bras Cir ; 46(5): e20192260, 2019.
Artigo em Português, Inglês | MEDLINE | ID: mdl-31859723

RESUMO

OBJECTIVE: to evaluate the use of a bacterial cellulose biopolymer film and gel dressing in the treatment of patients with ischemic wounds submitted to lower limb revascularization. METHODS: we conducted a randomized clinical trial in the Angiology and Vascular Surgery outpatient clinic of the Clinics Hospital of the Federal University of Pernambuco, between January 2017 and December 2018. We followed 24 patients after lower limb revascularization, divided into two groups: Experimental, treated with bacterial cellulose biopolymer film and gel, and Control, treated with essential fatty acids. Patients attended weekly appointments to change dressings and had their wound healing processes evaluated over a period of 90 days. RESULTS: the reduction of the ischemic wounds' areas after 30 days was 4.3cm2 (55%) on average for the experimental group, and the 5.5cm2 (48.5%) for the control group (p>0.05). The complete healing rate at 90 days was 34.8%, 50% in the experimental group and 18.2% in the control group (p=0.053). CONCLUSION: the bacterial cellulose biopolymer film associated with gel can be used as a dressing in the treatment of ischemic wounds of patients undergoing revascularization of the lower limbs.


Assuntos
Bandagens , Biopolímeros/uso terapêutico , Celulose/uso terapêutico , Isquemia/complicações , Isquemia/terapia , Extremidade Inferior/patologia , Cicatrização/efeitos dos fármacos , Idoso , Idoso de 80 Anos ou mais , Angioplastia , Feminino , Géis/uso terapêutico , Humanos , Isquemia/patologia , Extremidade Inferior/irrigação sanguínea , Masculino , Pessoa de Meia-Idade , Fatores de Tempo , Resultado do Tratamento
2.
Prensa méd. argent ; 105(9 especial): 521-525, oct 2019. tab
Artigo em Inglês | LILACS, BINACIS | ID: biblio-1046371

RESUMO

Over the last decade, the interest in using bacterial cellulose in medicine has increased. The article publishes the data about the efficiency of healing burn wounds in rabbits in experimental conditions with the use of the DermaRM wound dressing, compared to the traditionally used Panthenol ointment and the Branolind N salve dressing


Assuntos
Animais , Coelhos , Pomadas/uso terapêutico , Bandagens , Queimaduras/terapia , Celulose/uso terapêutico , Resultado do Tratamento , Tempo para o Tratamento
3.
Carbohydr Polym ; 219: 210-218, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31151519

RESUMO

Scaffold plays a critical role in stem cell differentiation and tissue regeneration. Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study. The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%. They were also favorable with cell attachment and growth. After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold. When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1). The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.


Assuntos
Celulose/química , Colágeno/química , Gluconacetobacter xylinus/metabolismo , Engenharia Tecidual/métodos , Tecidos Suporte/química , Animais , Regeneração Óssea/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Celulose/uso terapêutico , Colágeno/uso terapêutico , Humanos , Células-Tronco Mesenquimais/efeitos dos fármacos , Camundongos , Células NIH 3T3 , Osteogênese/efeitos dos fármacos
4.
Biomed Pharmacother ; 112: 108640, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30784929

RESUMO

Since early times, propolis has been used in folk medicine. The red propolis, collected in the northeast region of Brazil has been highlighted due to its popular use as an antimicrobial, with anti-inflammatory and healing properties, which are associated with its chemical composition. Here, we combine a bacterial membrane with red propolis to treat wounds of diabetic mice. This work aims to evaluate a biocurative from bacterial cellulose associated with red propolis in diabetic mice as wound healing model. Biocuratives from bacterial cellulose membrane and different extracts of red propolis were produced. The qualification and quantification of the presence of propolis chemical compounds in the membrane were investigated through high-resolution mass spectrometry (HRMS). Tests in vivo with biocuratives were performed on Swiss male diabetic mice induced by estroptozotocin. The animals were submitted to a surgical procedure and a single lesion was produced in the dorsal region, which was treated with the biocuratives. Macroscopic assessments were performed at 2, 7 and 14 postoperative days, and biopsies were collected on days 0, 7 and 14 for histological analysis, myeloperoxidase enzyme activity (MPO) and cytokines (TNF-α, IL-1ß, and TGF-ß). Altogether, ten compounds were identified in membranes and five were further quantified. The ethyl acetate extract showed more red propolis markers, and the most prevalent compound was Formononetin with 4423.00-2907.00 µg.g-1. Macroscopic analyses demonstrated that the two groups treated with red propolis (GMEBT and GMEAE) showed significantly greater healing capabilities compared to the control groups (GS and GMS). An increase in leukocyte recruitment was observed, confirmed by the activity of the enzyme myeloperoxidase (MPO) in GMEBT and GMEAE groups. The levels of TNF-α were significantly higher in wounds stimulated with red propolis, as well as in TGF-ß (GMEBT and GMEAE) on day 7. This was different from the IL-1ß levels that were higher in the control groups (GS and GMS). In summary, the biocuratives produced in this work were able to accelerate the wound healing process in a diabetic mouse model. In this way, the traditional knowledge of red propolis activity helped to create a biotechnological product, which can be used for diabetic wound healing purpose.


Assuntos
Acetobacteraceae/química , Celulose/uso terapêutico , Diabetes Mellitus Experimental/tratamento farmacológico , Própole/uso terapêutico , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Animais , Membrana Celular/química , Celulose/administração & dosagem , Celulose/isolamento & purificação , Diabetes Mellitus Experimental/complicações , Masculino , Própole/administração & dosagem , Pele/lesões , Resultado do Tratamento
5.
Int J Biol Macromol ; 127: 606-617, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30685305

RESUMO

The use of cellulose as an alternative to synthetic polymers might permit the decrease of environmental impacts by achieving more easily biodegradable or reusable materials. Recently, cellulose and inorganics are synergistically coupled to tailor promising functional hybrids with unique properties. The current review presents an overview of the recent trials for preparing cellulose/calcium phosphate hybrid materials and offers prospects for many new applications in diverse fields. During the whole review, different cellulose forms and cellulose derivatives are being explored as fillers or templates for the design of new functional materials and support for the loading of different calcium phosphate phases. The functionalization of cellulose with calcium phosphate was carried out to construct new and attractive physicochemical properties that are being exploited for different applications such as bone regeneration, drug delivery vehicles, dental repair and adsorption. The studying of cellulose/calcium phosphate hybrid materials is supported by increasing concern in providing smart materials that combine best of the calcium phosphate properties along with cellulose. The review also aims to study the driving forces and limitations towards structuring of cellulose/calcium phosphate hybrid materials. It appears that the use of cellulosic materials for constructing new hybrids is very promising for biomedical and environmental applications.


Assuntos
Fosfatos de Cálcio , Celulose , Portadores de Fármacos , Animais , Regeneração Óssea/efeitos dos fármacos , Fosfatos de Cálcio/química , Fosfatos de Cálcio/uso terapêutico , Celulose/química , Celulose/uso terapêutico , Portadores de Fármacos/química , Portadores de Fármacos/uso terapêutico , Humanos
6.
Int J Biol Macromol ; 124: 235-245, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30481535

RESUMO

Triblock poloxamer copolymer (PM) has been extensively utilized to deliver various ophthalmic pharmaceutical compounds. The aim of efficient ophthalmic drug delivery strategy is to attain the longer precorneal resident time and good bioavailability of drugs. In this pursuit, the influence of cellulose nanocrystals (CNC) on the in situ gelation behavior of PM and in vitro release of pilocarpine hydrochloride from the nanocomposites formulations was studied. The critical concentration of gelation of PM being 18% (wt/v) was dropped to 16.6% (wt/v) by the addition of a very low percentage of CNC. The reinforcing nature of CNC via H-bonding in the in situ nanocomposite gel also led to an increase in gel strength along with the sustained release of loaded drugs when compared with the pure PM gel. All formulations revealed that the drug release mechanism is controlled by the Fickian diffusion. Thus, the CNC has a significant effect on the gelation behavior, gel strength, and drug release kinetics of PM-CNC formulations.


Assuntos
Celulose/química , Oftalmopatias/tratamento farmacológico , Nanopartículas/química , Pilocarpina/química , Administração Oftálmica , Celulose/uso terapêutico , Composição de Medicamentos , Sistemas de Liberação de Medicamentos , Humanos , Nanocompostos/química , Nanocompostos/uso terapêutico , Nanopartículas/uso terapêutico , Pilocarpina/uso terapêutico , Poloxâmero/química , Poloxâmero/uso terapêutico , Polímeros/química , Polímeros/uso terapêutico
7.
Int Arch Allergy Immunol ; 178(3): 229-237, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30408809

RESUMO

BACKGROUND: Cellulose powder (CP) has been reported as a safe and effective complementary treatment for allergic rhinitis (AR). Currently, CP has gained increasing application for clinical management worldwide, particularly in China. However, studies focusing on the effect of CP on normal human nasal epithelial cells (hNECs) and ciliary function are lacking. Here, we aimed to explore the adverse effects of CP on the activity and ciliary function of hNECs. METHODS: We biopsied ethmoid sinus or middle turbinate tissues during surgical resection from control subjects who underwent endoscopic sinus surgery for diseases other than AR. Cells were isolated and passaged, followed by differentiation in an air-liquid interface (ALI). Flow cytometry and cell viability test (cell counting kit-8) were performed to detect the cytotoxicity of CP (effects on cell proliferation) on normal hNECs. By using the ALI culture model, we investigated the effects of CP on ciliary beat frequency (CBF). RESULTS: There was a significant reduction in hNEC count at high concentrations of CP (2.5 mg/mL) at days 3 and 7 (both p < 0.05). As the concentration increased, cell death increased progressively from day 3 to day 7. However, these effects were not evident at low concentrations (0.25 mg/mL, p > 0.05). High-dose CP (2.5 mg) significantly reduced the CBF (p < 0.05). At lower concentrations (0.25-2.5 mg/mL), CP initially increased but subsequently reduced the CBF of hNECs compared with control group. CONCLUSIONS: Cytotoxicity and the suppression of ciliary beat at high concentrations justify more prudent use of CP for the management of AR.


Assuntos
Celulose/farmacologia , Cílios/efeitos dos fármacos , Mucosa Nasal/efeitos dos fármacos , Adulto , Diferenciação Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Celulose/efeitos adversos , Celulose/uso terapêutico , Cílios/fisiologia , Relação Dose-Resposta a Droga , Células Epiteliais/efeitos dos fármacos , Feminino , Humanos , Masculino , Pós , Rinite Alérgica/tratamento farmacológico
8.
Int J Biol Macromol ; 122: 452-460, 2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30385344

RESUMO

Bacterial cellulose is well known for its excellent contributions in biomedical applications due to its superior properties. However the lack of antimicrobial property restricts its use in wound healing. To address the complications in third degree burns, thymol enriched bacterial cellulose hydrogel (BCT) was developed in this study. The incorporation of thymol into bacterial cellulose along with its chemical and thermal changes were investigated by FTIR, TGA and DSC respectively. Antimicrobial studies revealed that BCT possess excellent biocidal activity against burn specific pathogens. The in vitro biocompatibility studies were carried out in mouse 3T3 fibroblast cells. The BCT hydrogel facilitated the growth of fibroblast cells, exhibiting low toxicity, and increased cell viability. The burn wound healing efficiency of the BCT hydrogel was examined in vivo using female albino Wistar rats. Histopathological studies reveal that the wound treated with BCT hydrogel showed faster wound closure than BC and control groups. All these findings, suggest that BCT hydrogel can be used as resourceful and natural burn wound dressing material.


Assuntos
Queimaduras/fisiopatologia , Celulose/química , Celulose/farmacologia , Gluconacetobacter xylinus/química , Hidrogéis/química , Timol/química , Cicatrização/efeitos dos fármacos , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Queimaduras/tratamento farmacológico , Proliferação de Células/efeitos dos fármacos , Celulose/uso terapêutico , Feminino , Camundongos , Células NIH 3T3 , Ratos , Ratos Wistar
9.
Acta Biomater ; 84: 268-279, 2019 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-30465922

RESUMO

Glioblastoma (GB) is a highly infiltrative tumor, recurring, in 90% of cases, within a few centimeters of the surgical resection cavity, even with adjuvant chemo/radiotherapy. Residual GB cells left in the margins or infiltrating the brain parenchyma shelter behind the extremely fragile and sensitive brain tissue and may favor recurrence. Tools for eliminating these cells without damaging the brain microenvironment are urgently required. We propose a strategy involving the implantation, into the tumor bed after resection, of a scaffold to concentrate and trap these cells, to facilitate their destruction by targeted therapies, such as stereotactic radiosurgery. We used bacterial cellulose (BC), an easily synthesized and modifiable random nanofibrous biomaterial, to make the trap. We showed that the structure of BC membranes was ideal for trapping tumor cells and that BC implants were biocompatible with brain parenchyma. We also demonstrated the visibility of BC on magnetic resonance imaging, making it possible to follow its fate in clinical situations and to define the target volume for stereotactic radiosurgery more precisely. Furthermore, BC membranes can be loaded with chemoattractants, which were released and attracted tumor cells in vitro. This is of particular interest for trapping GB cells infiltrating tissues within a few centimeters of the resection cavity. Our data suggest that BC membranes could be a scaffold of choice for implantation after surgical resection to trap residual GB cells. STATEMENT OF SIGNIFICANCE: Glioblastoma is a highly infiltrative tumor, recurring, in 90% of cases, within a few centimeters of the surgical resection cavity, even with adjuvant chemo/radiotherapy. Residual tumor cells left in the margins or infiltrating the brain parenchyma shelter behind the extremely fragile and sensitive brain tissue and contribute to the risk of recurrence. Finding tools to eliminate these cells without damaging the brain microenvironment is a real challenge. We propose a strategy involving the implantation, into the walls of the surgical resection cavity, of a scaffold to concentrate and trap the residual tumor cells, to facilitate their destruction by targeted therapies, such as stereotactic radiosurgery.


Assuntos
Materiais Biocompatíveis , Neoplasias Encefálicas , Glioblastoma , Imagem por Ressonância Magnética , Membranas Artificiais , Nanofibras , Radiocirurgia , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/uso terapêutico , Encéfalo/diagnóstico por imagem , Encéfalo/metabolismo , Neoplasias Encefálicas/diagnóstico por imagem , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/radioterapia , Linhagem Celular Tumoral , Celulose/química , Celulose/uso terapêutico , Glioblastoma/diagnóstico por imagem , Glioblastoma/metabolismo , Glioblastoma/radioterapia , Humanos , Masculino , Nanofibras/química , Nanofibras/uso terapêutico , Ratos , Ratos Sprague-Dawley , Microambiente Tumoral/efeitos dos fármacos , Microambiente Tumoral/efeitos da radiação
10.
Macromol Biosci ; 19(2): e1800225, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30451373

RESUMO

Surgical clipping and endovascular coiling are well recognized as conventional treatments of Penetrating Brain Injury aneurysms. These clinical approaches show partial success, but often result in thrombus formation and the rupture of aneurysm near arterial walls. The authors address these challenging brain traumas with a unique combination of a highly biocompatible biopolymer hydrogel rendered magnetic in a flexible and resilient membrane coating integrated to a scaffold stent platform at the aneurysm neck orifice, which enhances the revascularization modality. This work focuses on the in situ diagnosis of nano-mechanical behavior of bacterial nanocellulose (BNC) membranes in an aqueous environment used as tissue reconstruction substrates for cerebral aneurysmal neck defects. Nano-mechanical evaluation, performed using instrumented nano-indentation, shows with very low normal loads between 0.01 to 0.5 mN, in the presence of deionized water. Mechanical testing and characterization reveals that the nano-scale response of BNC behaves similar to blood vessel walls with a very low Young´s modulus, E (0.0025 to 0.04 GPa), and an evident creep effect (26.01 ± 3.85 nm s-1 ). These results confirm a novel multi-functional membrane using BNC and rendered magnetic with local adhesion of iron-oxide magnetic nanoparticles.


Assuntos
Revascularização Cerebral/métodos , Procedimentos Endovasculares/métodos , Hidrogéis/uso terapêutico , Aneurisma Intracraniano/cirurgia , Nanopartículas de Magnetita/uso terapêutico , Celulose/uso terapêutico , Procedimentos Endovasculares/efeitos adversos , Gluconacetobacter xylinus/metabolismo , Humanos , Aneurisma Intracraniano/fisiopatologia , Fenômenos Mecânicos , Instrumentos Cirúrgicos
11.
Carbohydr Polym ; 206: 548-556, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30553356

RESUMO

Bacterial cellulose (BC) is a promising biopolymer with wound healing and tissue regenerative properties but lack of antimicrobial property limits its biomedical applications. Therefore, current study was proposed to combine wound healing property of BC with antimicrobial activity of montmorillonite (MMT) and modified montmorillonites (Cu-MMT, Na-MMT and Ca-MMT) to design novel artificial substitute for burns. Designed nanocomposites were characterized through Fe-SEM, FTIR and XRD. The antimicrobial activities of composites were tested against Escherichia coli, Salmonella typhimurium, Citrobacter fruendii, Pseudomonas aeruginosa, Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus. Tissue regeneration and wound healing activities of the composites were assessed in burn mice model. Physico-chemical characterization confirmed the loading of MMT onto surface and BC matrix. Modified MMTs-BC nanocomposites showed clear inhibitory zone against the tested pathogens. Animals treated with modified MMTs-BC nanocomposites exhibited enhanced wound healing activity with tissue regeneration, reepithelialization, healthy granulation and vascularization. These findings demonstrated that modified MMTs-BC nanocomposites could be used as a novel artificial skin substitute for burn patients and scaffold for skin tissue engineering.


Assuntos
Antibacterianos/uso terapêutico , Bentonita/uso terapêutico , Materiais Biocompatíveis/uso terapêutico , Queimaduras/tratamento farmacológico , Celulose/uso terapêutico , Nanocompostos/uso terapêutico , Animais , Antibacterianos/síntese química , Antibacterianos/química , Antibacterianos/farmacologia , Bentonita/síntese química , Bentonita/química , Bentonita/farmacologia , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Cálcio/química , Celulose/síntese química , Celulose/química , Celulose/farmacologia , Cobre/química , Bactérias Gram-Negativas/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Camundongos Endogâmicos BALB C , Nanocompostos/química , Regeneração/efeitos dos fármacos , Fenômenos Fisiológicos da Pele , Pele Artificial , Sódio/química , Cicatrização/efeitos dos fármacos
12.
PLoS One ; 13(10): e0205205, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30356274

RESUMO

Herein we present an innovative method of coating the surface of Titanium-Aluminium-Niobium bone scaffold implants with bacterial cellulose (BC) polymer saturated with antibiotic. Customized Ti6Al7Nb scaffolds manufactured using Selective Laser Melting were immersed in a suspension of Komagataeibacter xylinus bacteria which displays an ability to produce a 3-dimensional structure of bio-cellulose polymer. The process of complete implant coating with BC took on average 7 days. Subsequently, the BC matrix was cleansed by means of alkaline lysis and saturated with gentamycin. Scanning electron microscopy revealed that BC adheres and penetrates into the implant scaffold structure. The viability and development of the cellular layer on BC micro-structure were visualized by means of confocal microscopy. The BC-coated implants displayed a significantly lower cytotoxicity against osteoblast and fibroblast cell cultures in vitro in comparison to non-coated implants. It was also noted that gentamycin released from BC-coated implants inhibited the growth of Staphylococcus aureus cultures in vitro, confirming the suitability of such implant modification for preventing hostile microbial colonization. As demonstrated using digital microscopy, the procedure used for implant coating and BC chemical cleansing did not flaw the biomaterial structure. The results presented herein are of high translational value with regard to future use of customized, BC-coated and antibiotic-saturated implants designed for use in orthopedic applications to speed up recovery and to reduce the risk of musculoskeletal infections.


Assuntos
Celulose/química , Materiais Revestidos Biocompatíveis/química , Gentamicinas/química , Titânio/química , Antibacterianos/química , Antibacterianos/uso terapêutico , Desenvolvimento Ósseo/efeitos dos fármacos , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/microbiologia , Celulose/uso terapêutico , Materiais Revestidos Biocompatíveis/uso terapêutico , Humanos , Osteoblastos/química , Próteses e Implantes/microbiologia , Staphylococcus aureus , Tecidos Suporte , Titânio/uso terapêutico
13.
J Tissue Viability ; 27(4): 249-256, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30318397

RESUMO

Burns are injuries caused mainly by thermal trauma, which can progress to unsatisfactory results healing. This study aimed to evaluate the biomaterial (bacterial cellulose membrane) and photobiomodulation, exclusively and associated, in the treatment of third degree burns in rats. Forty male Wistar rats (±280 g) were randomly divided into four groups, with 10 animals each: control group (CG); bacterial cellulose membrane group (MG); laser group (LG) and bacterial cellulose membrane and laser group (MG + L). The burn was caused with a 1 cm2 aluminum plate heated to 150 °C and pressed on the animal's back for 10 s. The treatments were started immediately after induction of injury. For to laser irradiation (660 nm, 100 mW, 25 J/cm2 and energy of 1 J) on five distinct application points were used, on alternate days, a total of five sessions. After ten days of treatment the animals were euthanized for collected samples. One-way ANOVA and Tukey's tests (P < 0.05) were used. Histological analysis revealed differences regarding the healing process phase in each experimental group. MG showed the proliferative phase. The LG demonstrated greater amount of blood vessels and immune expression of VEGF. However, when the treatments were combined, the number of vessels and the immune expression of VEGF factor was lower than LG. Thus, it was concluded that both treatments proposed (biomaterial and LLLT) are good alternatives for third degree burns when applied isolated because they stimulate the healing process by acting on the modulation of the inflammatory phase and promote stimulation of angiogenesis.


Assuntos
Queimaduras/terapia , Celulose/farmacologia , Terapia com Luz de Baixa Intensidade/normas , Cicatrização/efeitos da radiação , Análise de Variância , Animais , Celulose/administração & dosagem , Celulose/uso terapêutico , Ciclo-Oxigenase 2/análise , Modelos Animais de Doenças , Terapia com Luz de Baixa Intensidade/métodos , Masculino , Ratos , Ratos Wistar , Fator A de Crescimento do Endotélio Vascular/análise
14.
Arch Dermatol Res ; 310(10): 795-805, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30302557

RESUMO

Bacterial cellulose wound dressings containing silk sericin and PHMB (BCSP) were developed in our previous studies. It had good physical properties, efficacy, and safety. For further use as a medical material, this dressing was investigated for its efficacy and safety in split-thickness skin graft (STSG) donor-site wound treatment compared to Bactigras® (control). Moreover, the inflammatory responses to both dressings were also deeply investigated. For in vivo study, expressions of anti-inflammatory cytokines were intensely considered in the tissue interfacing area. The result showed that IL-4 and TGF-ß from BCSP-treated tissue had advantages over Bactigras®-treated tissue at 14 and 21 days post-implantation. For clinical study, a single-blinded, randomized controlled study was generated. The half of STSG donor site wound was randomly assigned to cover with BCSP or Bactigras®. Twenty-one patients with 32 STSG donor site wounds were enrolled. The results showed that wound-healing time was not significantly different in both dressings. However, wound quality of BCSP was better than Bactigras® at healing time and after 1 month (p < 0.05). The pain scores of BCSP-treated wound were statistically significant lower than Bactigras®-treated wound (p < 0.05). No sign of infection or adverse event was observed after treatment with both dressings. In conclusion, the inflammation responses of the dressing were clearly clarified. The advantages of BCSP were wound-quality improvement, pain reduction, and infection protection without adverse events. It was fit to be used as the alternative treatment of STSG donor site wound.


Assuntos
Bandagens/efeitos adversos , Biguanidas/uso terapêutico , Celulose/uso terapêutico , Desinfetantes/uso terapêutico , Polissacarídeos Bacterianos/uso terapêutico , Sericinas/uso terapêutico , Cicatrização/efeitos dos fármacos , Eritema/induzido quimicamente , Feminino , Humanos , Inflamação/induzido quimicamente , Interleucina-4/metabolismo , Masculino , Pessoa de Meia-Idade , Transplante de Pele/métodos , Fator de Crescimento Transformador beta1/metabolismo , Resultado do Tratamento
15.
Vet J ; 241: 38-41, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30340658

RESUMO

Infectious ocular disease, such as conjunctivitis, is common in cats and can be caused by several viruses and bacteria, either as a single infection or as co-infections. In this study, povidone-iodine (PVP-I), alone or compounded with hydroxyethyl cellulose (HEC), was investigated for its efficacy against these pathogens in vitro. Whilst PVP-I alone was effective at inhibiting feline herpesvirus type 1 (FHV-1), Chlamydia felis, and Mycoplasma felis, PVP-I with HEC exerted a synergistic inhibitory effect against FHV-1 and C. felis. In contrast, only minimal inhibition of feline calicivirus was observed. These results demonstrate that PVP-I, alone and in combination with HEC, is effective against some feline ocular pathogens when tested in cell lines in vitro. In vivo studies investigating the systemic safety, ocular tolerance, and clinical efficacy of this combination in cats would be necessary before it could be recommended as a therapy in affected cats.


Assuntos
Anti-Infecciosos Locais/uso terapêutico , Doenças do Gato/tratamento farmacológico , Celulose/análogos & derivados , Conjuntivite/veterinária , Soluções Oftálmicas/uso terapêutico , Povidona-Iodo/uso terapêutico , Animais , Anti-Infecciosos Locais/administração & dosagem , Anti-Infecciosos Locais/farmacologia , Calicivirus Felino/efeitos dos fármacos , Doenças do Gato/microbiologia , Gatos , Celulose/administração & dosagem , Celulose/uso terapêutico , Chlamydia/efeitos dos fármacos , Infecções por Chlamydophila/tratamento farmacológico , Infecções por Chlamydophila/veterinária , Conjuntivite/tratamento farmacológico , Quimioterapia Combinada , Feminino , Infecções por Herpesviridae/tratamento farmacológico , Infecções por Herpesviridae/veterinária , Masculino , Testes de Sensibilidade Microbiana/veterinária , Mycoplasma/efeitos dos fármacos , Infecções por Mycoplasma/tratamento farmacológico , Infecções por Mycoplasma/veterinária , Soluções Oftálmicas/administração & dosagem , Povidona-Iodo/administração & dosagem , Povidona-Iodo/farmacologia , Resultado do Tratamento
16.
Acta Cir Bras ; 33(8): 673-683, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30208129

RESUMO

PURPOSE: To evaluate the efficacy of the cellulosic exopolysaccharide membrane (CEM) as a urethral reinforcement for urethrovesical anastomosis. METHODS: Twenty eight rabbits were submitted to urethrovesical anastomosis with or without CEM reinforcement. The animals were divided into 4 groups: C7, CEM7, C14 and CEM14: (C= only anastomosis or CEM = anastomosis + CEM), evaluated after 7 weeks, and 14 weeks. The biointegration and biocompatibility of CEM were evaluated according to stenosis, fistula, urethral wall thickness, urethral epithelium, rate of inflammation and vascularization. RESULTS: Between the two experimental groups, the difference in the number of stenosis or urinary fistula was not statistically significant. The morphometric analysis revealed preservation of urethral lumen, well adhered CEM without extrusion, a controlled inflammatory process and implant vascularization. The urothelium height remained constant over time after CEM reinforcement and the membrane wall was thicker, statistically, after 14 weeks. CONCLUSION: The absence of extrusion, stenosis or urinary fistula after 14 weeks of urethrovesical anastomosis demonstrates cellulosic exopolysaccharide membrane biocompatibility and biointegration with tendency to a thicker wall.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Celulose/uso terapêutico , Polissacarídeos Bacterianos/uso terapêutico , Uretra/cirurgia , Bexiga Urinária/cirurgia , Anastomose Cirúrgica , Animais , Celulose/biossíntese , Microbiologia Industrial/métodos , Masculino , Teste de Materiais , Neovascularização Patológica , Coelhos , Reprodutibilidade dos Testes , Fatores de Tempo , Pesquisa Médica Translacional , Resultado do Tratamento , Uretra/patologia , Bexiga Urinária/patologia
17.
Kidney Blood Press Res ; 43(5): 1472-1478, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30235456

RESUMO

BACKGROUND/AIMS: A recent alert from Spanish health authorities warned of a higher incidence of reported hypersensitivity reactions to hemodialysis membranes with polysulfone, in the 2017 review of acute reactions to dialyzers found only published reports in the 21st century on polysulfone and its derivatives. The aim is to assess/evaluate the current incidence and characteristics of hypersensitivity reactions in hemodialysis patients. METHODS: A retrospective multicentre study in 9 Spanish hospitals evaluated patients in whom a hypersensitivity reaction required a change in dialyzer membrane. RESULTS: A total of 37 patients out of 1561 (2.37%) had hypersensitivity reactions and clinical, epidemiological and analytical data were available for 33 patients (2.11%). The membranes involved were polysulfone (n=23), polynephron (n=8), polyethersulfone (n=1) and polyacrylonitrile (n=1). This distribution reflected the frequency of use of membranes in the participating dialysis units. The reactions were described as type A in 18 cases and type B in 15 cases. There were no significant differences between the two types in clinical symptoms, the composition of the membrane involved, the method of sterilization, the season, or the time during the session in which they occurred. The most frequent symptom was dyspnea/breathlessness (64% of reactions). Eosinophilia was common (74%). 54% of the reactions occurred within the first 30 minutes of hemodialysis, 64% occurred during the first year of dialysis, and 54% required discontinuation of dialysis session. Cellulose triacetate was used as an alternative dialyzer in 78% of the cases. CONCLUSION: The incidence of hypersensitivity reactions was in the range found in reports from 20 years ago and is observed associated with synthetic membranes, not just polysulfones. Cellulose triacetate appears to be a good alternative for these patients.


Assuntos
Hipersensibilidade/etiologia , Diálise Renal/efeitos adversos , Resinas Acrílicas , Idoso , Idoso de 80 Anos ou mais , Celulose/análogos & derivados , Celulose/imunologia , Celulose/uso terapêutico , Feminino , Humanos , Masculino , Membranas Artificiais , Pessoa de Meia-Idade , Polímeros , Diálise Renal/instrumentação , Estudos Retrospectivos , Sulfonas/imunologia
18.
Mol Biol Rep ; 45(6): 2857-2867, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30094529

RESUMO

Wound is a growing healthcare challenge affecting several million worldwide. Lifestyle disorders such as diabetes increases the risk of wound complications. Effective management of wound is often difficult due to the complexity in the healing process. Addition to the conventional wound care practices, the bioactive polymers are gaining increased importance in wound care. Biopolymers are naturally occurring biomolecules synthesized by microbes, plants and animals with highest degree of biocompatibility. The bioactive properties such as antimicrobial, immune-modulatory, cell proliferative and angiogenic of the polymers create a microenvironment favorable for the healing process. The versatile properties of the biopolymers such as cellulose, alginate, hyaluronic acid, collagen, chitosan etc have been exploited in the current wound care market. With the technological advances in material science, regenerative medicine, nanotechnology, and bioengineering; the functional and structural characteristics of biopolymers can be improved to suit the current wound care demands such as tissue repair, restoration of lost tissue integrity and scarless healing. In this review we highlight on the sources, mechanism of action and bioengineering approaches adapted for commercial exploitation.


Assuntos
Biopolímeros/uso terapêutico , Engenharia Tecidual/métodos , Cicatrização/efeitos dos fármacos , Alginatos/uso terapêutico , Animais , Celulose/uso terapêutico , Quitosana/uso terapêutico , Colágeno/uso terapêutico , Humanos , Ácido Hialurônico/uso terapêutico , Medicina Regenerativa/métodos , Pele/metabolismo , Transplante de Pele/métodos
19.
Acta cir. bras ; 33(8): 673-683, Aug. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-949378

RESUMO

Abstract Purpose: To evaluate the efficacy of the cellulosic exopolysaccharide membrane (CEM) as a urethral reinforcement for urethrovesical anastomosis. Methods: Twenty eight rabbits were submitted to urethrovesical anastomosis with or without CEM reinforcement. The animals were divided into 4 groups: C7, CEM7, C14 and CEM14: (C= only anastomosis or CEM = anastomosis + CEM), evaluated after 7 weeks, and 14 weeks. The biointegration and biocompatibility of CEM were evaluated according to stenosis, fistula, urethral wall thickness, urethral epithelium, rate of inflammation and vascularization. Results: Between the two experimental groups, the difference in the number of stenosis or urinary fistula was not statistically significant. The morphometric analysis revealed preservation of urethral lumen, well adhered CEM without extrusion, a controlled inflammatory process and implant vascularization. The urothelium height remained constant over time after CEM reinforcement and the membrane wall was thicker, statistically, after 14 weeks. Conclusion: The absence of extrusion, stenosis or urinary fistula after 14 weeks of urethrovesical anastomosis demonstrates cellulosic exopolysaccharide membrane biocompatibility and biointegration with tendency to a thicker wall.


Assuntos
Animais , Masculino , Coelhos , Uretra/cirurgia , Materiais Biocompatíveis/uso terapêutico , Bexiga Urinária/cirurgia , Celulose/uso terapêutico , Polissacarídeos Bacterianos/uso terapêutico , Fatores de Tempo , Uretra/patologia , Bexiga Urinária/patologia , Microbiologia Industrial/métodos , Teste de Materiais , Anastomose Cirúrgica , Celulose/biossíntese , Reprodutibilidade dos Testes , Resultado do Tratamento , Pesquisa Médica Translacional , Neovascularização Patológica
20.
Burns ; 44(8): 1982-1988, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30005989

RESUMO

BACKGROUND: The current treatment for partial thickness burns at the trial site is silver sulphadiazine, as it minimises bacterial colonisation of wounds. Its deleterious effect on wound healing, together with the need for repeated, often painful, procedures, has brought about the search for a better treatment. Microbial cellulose has shown promising results that avoid these disadvantages. The aim of this study was therefore to compare microbial cellulose with silver sulphadiazine as a dressing for partial thickness burns. METHOD: All patients who presented with partial thickness (superficial and deep dermal) burns from October 2014 to October 2016 were screened for this randomised clinical trial. Twenty patients were included in each group: the cellulose group was treated with microbial cellulose sheets and the control group with silver sulphadiazine cream 10mg/g. The wound was evaluated every third day. Pain was assessed using the Face, Legs, Activity, Cry, Consolability (FLACC) scale during and after each procedure. Other variables recorded were age, sex, percentage total body surface area burned (TBSA%), clinical signs of infection, time for epithelialisation and hospital stay. Linear multivariable regression was used to analyse the significance of differences between the treatment groups by adjusting for the size and depth of the burn, and the patient's age. RESULTS: Median TBSA% was 9% (IQR 5.5-12.5). The median number of dressing changes was 1 (IQR 1-2) in the cellulose group, which was lower than that in the control group (median 9.5, IQR 6-16) (p<0.001). Multivariable regression analysis showed that the group treated with microbial cellulose spent 6.3 (95% CI 0.2-12.5) fewer days in hospital (p=0.04), had a mean score that was 3.4 (95% CI 2.5-4.3) points lower during wound care (p<0.001), and 2.2 (95% CI 1.6-2.7) afterwards (p<0.001). Epithelialisation was quicker, but not significantly so. CONCLUSION: These results suggest that the microbial cellulose dressing is a better first choice for treatment of partial thickness burns than silver sulphadiazine cream. Fewer dressings of the wound were done and, combined with the low pain scores, this is good for both the patients and the health care system. The differences in randomisation of the area of burns is, however, a concern that needs to be included in the interpretation of the results.


Assuntos
Anti-Infecciosos Locais/uso terapêutico , Bandagens , Queimaduras/terapia , Celulose/uso terapêutico , Sulfadiazina de Prata/uso terapêutico , Adolescente , Adulto , Superfície Corporal , Queimaduras/patologia , Criança , Feminino , Humanos , Modelos Lineares , Masculino , Análise Multivariada , Medição da Dor , Estudos Prospectivos , Reepitelização , Adulto Jovem
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