Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 617
Filtrar
1.
Res Vet Sci ; 137: 262-273, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34052571

RESUMO

The usage of materials with the potential to accelerate wound healing is a great benefit for patients and health care systems. This study evaluated the impact of using graphene oxide (GO)-cellulose nanocomposite on skin wound healing via in vitro and in vivo investigations. The nanomaterial was synthesized and characterized. Cytocompatibility performance of the GO-cellulose was investigated through in vitro testing based on MTT and live/dead assays by EA.hy926 human endothelial cells (ECs). Additionally, the effect of GO-cellulose on induced wound scratch model using EA.hy926 ECs was investigated. Finally, the therapeutic effect of GO-cellulose was evaluated in vivo after the creation of two full-thickness wounds in the dorsum of rats (8 mm diameter). These wounds were randomly placed into two groups, the control group (10 wounds) and the GO-cellulose group (10 wounds), and monitored for gross and histopathological changes at 7 and 21 days after wound induction. MTT and Live/Dead assays showed excellent GO-cellulose cytocompatibility, whereas no difference in ECs viability was observed after culturing using conditioned media. GO-cellulose nanocomposite enhanced cell migration in the in vitro wound scratch assay. As compared to the control group, the GO-cellulose nanocomposite group's wound healing process was promoted in the in vivo rat skin wounds. Interestingly, wound re-epithelization and neovascularization were significantly accelerated in the GO-cellulose-treated rats. Furthermore, thick granulation tissue formation and intense collagen deposition were found in the GO-cellulose group. These findings showed that GO-cellulose has a promoting effect on skin wound healing, suggesting its promising and potential application in tissue regeneration.


Assuntos
Celulose/uso terapêutico , Grafite/uso terapêutico , Nanocompostos/uso terapêutico , Cicatrização/efeitos dos fármacos , Animais , Colágeno/farmacologia , Células Endoteliais/efeitos dos fármacos , Humanos , Masculino , Ratos , Pele
2.
J Wound Care ; 30(4): 304-310, 2021 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-33856908

RESUMO

OBJECTIVE: A pressure ulcer (PU) is an area of tissue trauma caused by continuous and prolonged pressure, often associated with hospitalised patients immobilised due to neurological problems, negatively affecting their quality of life, and burdening the public budget. The aim of this study was to report the follow-up, for 45 weeks, of three patients with neurological lesions due to trauma who subsequently developed PUs, and who were treated with a combination of photodynamic therapy (PDT), low level laser therapy (LLLT) and cellulose membrane (CM). METHOD: PDT was mediated by the photosensitiser curcumin on a 1.5% emulsion base. Blue LED light at 450 nm was delivered continuously for 12 minutes at an irradiance of 30mW/cm2 and total energy delivered to the tissue was 22J/cm2. LLLT was performed with 660 nm laser, punctuated and continuous, twice a week with parameters: spot size 0.04cm2, power of 40mW, 10 seconds per point, fluence of 10J/cm2 and irradiance of 1000mW/cm2. RESULTS: All PUs had a significant reduction (range: 95.2-100%) of their area after 45 weeks of follow-up and two PUs had complete healing at 20 weeks and 30 weeks. All of the PUs showed a reduction in contamination with the PDT treatments in different proportions. CONCLUSION: From the results obtained, we conclude that the combination of PDT, LLLT and CM is a promising treatment for PU healing.


Assuntos
Celulose/uso terapêutico , Terapia com Luz de Baixa Intensidade , Fotoquimioterapia , Lesão por Pressão/terapia , Adulto , Seguimentos , Humanos , Masculino , Lesão por Pressão/psicologia , Qualidade de Vida
3.
J Wound Care ; 29(Sup12): S33-S37, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33320764

RESUMO

OBJECTIVE: Controlling infection and promoting healing should be the aims of hard-to-heal diabetic ulcer treatment, along with improving a patient's general condition and their blood sugar control. Many hard-to-heal diabetic ulcers present with cavities, tracks or a combination of these. There is a new biocellulose (with a nanosilver dressing) which has the ability to contour around and conform to the irregular surface of a wound bed. The purpose of this study was to evaluate its efficacy compared with a silver sulfadiazine cream, for hard-to-heal diabetic ulcer treatment. METHODS: In this randomised control trial, patients with hard-to-heal diabetic ulcers were divided into two equal-sized groups: treatment with the biocellulose with blue nanosilver (experimental group), and treatment with silver sulfadiazine cream group (control group). Cotton gauze was used as the secondary dressing for both groups. Demographic data, wound size, wound classification, wound photography and bacterial cultures were recorded at the beginning of the study. Wounds were debrided as necessary. Dressings were changed twice daily in the control group, and every three days in the experimental group. RESULTS: A total of 20 patients took part in the study (10 patients in each group). The highest mean wound healing rates were 91.4% in the experimental group and 83.9% in the control group. No wound infections or adverse effects from the dressings were detected in either group. CONCLUSION: In this study, biocellulose with blue nanosilver adapted well to the wound bed. Wound reduction was greater in the experimental group than the control group. Biocellulose with blue nanosilver could therefore be a good choice for hard-to-heal diabetic ulcer treatment, due to its good healing rates and minimal care requirements.


Assuntos
Bandagens , Celulose/uso terapêutico , Compostos de Prata/uso terapêutico , Sulfadiazina de Prata/uso terapêutico , Úlcera/terapia , Infecção dos Ferimentos/prevenção & controle , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nitrato de Prata/uso terapêutico , Resultado do Tratamento , Cicatrização , Adulto Jovem
4.
Biomolecules ; 10(10)2020 09 27.
Artigo em Inglês | MEDLINE | ID: mdl-32992554

RESUMO

Acute and chronic skin wounds due to burns, pressure injuries, and trauma represent a substantial challenge to healthcare delivery with particular impacts on geriatric, paraplegic, and quadriplegic demographics worldwide. Nevertheless, the current standard of care relies extensively on preventive measures to mitigate pressure injury, surgical debridement, skin flap procedures, and negative pressure wound vacuum measures. This article highlights the potential of adipose-, blood-, and cellulose-derived products (cells, decellularized matrices and scaffolds, and exosome and secretome factors) as a means to address this unmet medical need. The current status of this research area is evaluated and discussed in the context of promising avenues for future discovery.


Assuntos
Queimaduras/terapia , Exossomos/transplante , Hidrogéis/uso terapêutico , Cicatrização/genética , Queimaduras/patologia , Terapia Baseada em Transplante de Células e Tecidos/tendências , Celulose/uso terapêutico , Exossomos/genética , Humanos , Hidrogéis/química , Transplante de Células-Tronco Mesenquimais/tendências , Células-Tronco Mesenquimais/citologia , Pele/crescimento & desenvolvimento , Pele/lesões , Pele/metabolismo
5.
Molecules ; 25(19)2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32993007

RESUMO

The preparation of microcapsules composed by natural materials have received great attention, as they represent promising systems for the fabrication of micro-containers for controlled loading and release of active compounds, and for other applications. Using polysaccharides as the main materials is receiving increasing interest, as they constitute the main components of the plant cell wall, which represent an ideal platform to mimic for creating biocompatible systems with specific responsive properties. Several researchers have recently described methods for the preparation of microcapsules with various sizes and properties using cell wall polysaccharide nanomaterials. Researchers have focused mostly in using cellulose nanomaterials as structural components in a bio-mimetic approach, as cellulose constitutes the main structural component of the plant cell wall. In this review, we describe the microcapsules systems presented in the literature, focusing on the works where polysaccharide nanomaterials were used as the main structural components. We present the methods and the principles behind the preparation of these systems, and the interactions involved in stabilizing the structures. We show the specific and stimuli-responsive properties of the reported microcapsules, and we describe how these characteristics can be exploited for specific applications.


Assuntos
Celulose , Portadores de Fármacos , Nanoestruturas , Cápsulas , Celulose/química , Celulose/uso terapêutico , Portadores de Fármacos/química , Portadores de Fármacos/uso terapêutico , Nanoestruturas/química , Nanoestruturas/uso terapêutico , Propriedades de Superfície
6.
Carbohydr Polym ; 247: 116683, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32829811

RESUMO

Cellulose derivatives represent a viable alternative to pure cellulose due to their solubility in water and common organic solvents. This, coupled with their low cost, biocompatibility, and biodegradability, makes them an attractive choice for applications related to the biomedicine and bioanalysis area. Cellulose derivatives-based composites with improved properties were researched as films and membranes for osseointegration, hemodialysis and biosensors, smart textile fibers, tissue engineering scaffolds, hydrogels and nanoparticles for drug delivery. The different preparation strategies of these polymeric composites as well as the most recent available experimental results were described in this review. General aspects such as structure and properties of cellulose extracted from plants or bacterial sources, types of cellulose derivatives and their synthesis methods were also discussed. Finally, the future perspectives related to composites based on cellulose derivatives were highlighted and some conclusions regarding the reviewed applications were drawn.


Assuntos
Celulose/análogos & derivados , Celulose/química , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/uso terapêutico , Técnicas Biossensoriais/métodos , Celulose/isolamento & purificação , Celulose/uso terapêutico , Humanos , Hidrogéis/síntese química , Hidrogéis/química , Hidrogéis/uso terapêutico , Nanopartículas/química , Nanopartículas/uso terapêutico , Polímeros/síntese química , Polímeros/química , Polímeros/uso terapêutico , Tecidos Suporte/química
7.
Sensors (Basel) ; 20(7)2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32268471

RESUMO

We demonstrate the viability of using ultra-thin sheets of microbially grown nanocellulose to build functional medical sensors. Microbially grown nanocellulose is an interesting alternative to plastics, as it is hydrophilic, biocompatible, porous, and hydrogen bonding, thereby allowing the potential development of new application routes. Exploiting the distinguishing properties of this material enables us to develop solution-based processes to create nanocellulose printed circuit boards, allowing a variety of electronics to be mounted onto our nanocellulose. As proofs of concept, we have demonstrated applications in medical sensing such as heart rate monitoring and temperature sensing-potential applications fitting the wide-ranging paradigm of a future where the Internet of Things is dominant.


Assuntos
Materiais Biocompatíveis/química , Técnicas Biossensoriais , Celulose/química , Nanoestruturas/química , Materiais Biocompatíveis/uso terapêutico , Temperatura Corporal , Celulose/uso terapêutico , Frequência Cardíaca/fisiologia , Humanos , Internet das Coisas , Monitorização Fisiológica/tendências , Nanoestruturas/uso terapêutico
8.
Adv Wound Care (New Rochelle) ; 9(4): 199-210, 2020 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-32117583

RESUMO

Objective: Skin graft donor site management is a concern particularly for elderly patients and patients with poor wound healing competence, and also because donor sites are a source of pain and discomfort. Although different types of dressings exist, there is no consensus regarding optimal dressing type on donor site care to promote healing, reduce pain, and improve patients' comfort. Approach: This prospective, single-center clinical trial evaluated the performance of nanofibrillar cellulose (NFC) wound dressing (FibDex® by UPM-Kymmene Corporation) for treatment of donor sites compared with a polylactide-based copolymer dressing. The study enrolled 24 patients requiring skin grafting with mean age of 49 ± 18. The primary outcome measure was wound healing time. Secondary outcomes, the epithelialization, subjective pain, the scar appearance assessed using the Patient and Observer Scar Assessment Scale (POSAS), and skin elasticity and transepidermal water loss (TEWL), were evaluated at 1 and 6 months postoperatively. Results: No statistically significant differences were observed between NFC and copolymer dressings regarding wound healing time, epithelialization, experience of pain, or TEWL. Significant differences were observed in the POSAS results for thickness and vascularity in the Observer score, in the favor of NFC over copolymer dressing. Moreover, skin elasticity was significantly improved with NFC dressing in terms of viscoelasticity and elastic modulus at 1 month postoperatively. Innovation: NFC dressing is a new, green sustainable product for wound treatment without animal or human-origin components. Conclusion: NFC dressing provides efficient wound healing at skin graft donor sites and is comparable or even preferable compared with the copolymer dressing.


Assuntos
Bandagens , Queimaduras/cirurgia , Celulose/uso terapêutico , Hidrogéis/uso terapêutico , Reepitelização/efeitos dos fármacos , Transplante de Pele/métodos , Sítio Doador de Transplante , Adulto , Idoso , Feminino , Humanos , Hidrogéis/química , Masculino , Membranas Artificiais , Pessoa de Meia-Idade , Poliésteres/uso terapêutico , Estudos Prospectivos , Resultado do Tratamento
9.
Carbohydr Polym ; 231: 115727, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31888849

RESUMO

Carbazate groups were grafted on the commercial cellulose membrane (CM) to specifically scavenge the carbonylated proteins for hemodialysis. It confirmed that carbazate groups were successfully covalently attached on the CMs by XPS and EDS, and the modified CMs still saved their original morphology and crystalline structures by SEM and XRD. Furthermore, the modified CMs presented favorable physicochemical stability at wide pH range from 2.5 to 7.4. It was also found that the carbazate modified CMs could selectively remove carbonylated proteins from acrolein treated bovine serum albumin (BSA) or ESRD patient's blood serum in PBS buffer. The modified CMs showed the potential to be utilized as the substitute of dialysis membranes in hemodialysis.


Assuntos
Celulose/química , Hidrazinas/química , Falência Renal Crônica/terapia , Membranas Artificiais , Acroleína/farmacologia , Celulose/uso terapêutico , Humanos , Hidrazinas/uso terapêutico , Falência Renal Crônica/sangue , Polissacarídeos/química , Carbonilação Proteica/efeitos dos fármacos , Diálise Renal/instrumentação , Soroalbumina Bovina/química
11.
Eur Arch Otorhinolaryngol ; 277(1): 277-283, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31595316

RESUMO

PURPOSE: Functionality of the facial nerve is cosmetically important. While many techniques have been investigated, early and effective treatment for traumatic facial nerve paralysis remains challenging. Here, we aim to examine bacterial cellulose (BC) as a new tubularization material for improving facial nerve regeneration. METHODS: Our study was performed on 40 female Sprague Dawley rats. Rats were randomly divided into four groups, with 10 rats per group. In all rats, the main trunk of the facial nerve was completely cut 8 mm before the branching point. For repairing the facial nerve, in group 1, the nerve was left to recover spontaneously (control group); in group 2, it was repaired by primary suturing (8.0 Ethilon sutures, Ethicon); in group 3, BC tubes alone were used to aid nerve repair; and in group 4, both BC tubes and primary sutures (8.0 Ethilon sutures) were used. After 10 weeks, the facial nerve regeneration was evaluated by the whisker movement test and electrophysiologically (nerve stimulation threshold and compound muscle action potential). Nerve regeneration was assessed by calculating the number of myelinated nerve fibers, and by microscopically evaluating the amount of regeneration and fibrosis. RESULTS: No significant difference was observed among the groups in terms of whisker movement and electrophysiological parameters (P > 0.05). We found that the numbers of regenerating myelinated fibers were significantly increased (P < 0.05) when BC tubes were used as a nerve conduit. CONCLUSIONS: BC can be easily shaped into a hollow tube that guides nerve axons, resulting in better nerve regeneration after transection.


Assuntos
Celulose , Traumatismos do Nervo Facial/cirurgia , Regeneração Tecidual Guiada/instrumentação , Regeneração Nervosa/fisiologia , Procedimentos Neurocirúrgicos/instrumentação , Tecidos Suporte , Animais , Celulose/uso terapêutico , Modelos Animais de Doenças , Nervo Facial/cirurgia , Feminino , Regeneração Tecidual Guiada/métodos , Procedimentos Neurocirúrgicos/métodos , Ratos , Ratos Sprague-Dawley , Vibrissas/inervação
13.
Rev Col Bras Cir ; 46(5): e20192260, 2019.
Artigo em Português, Inglês | MEDLINE | ID: mdl-31859723

RESUMO

OBJECTIVE: to evaluate the use of a bacterial cellulose biopolymer film and gel dressing in the treatment of patients with ischemic wounds submitted to lower limb revascularization. METHODS: we conducted a randomized clinical trial in the Angiology and Vascular Surgery outpatient clinic of the Clinics Hospital of the Federal University of Pernambuco, between January 2017 and December 2018. We followed 24 patients after lower limb revascularization, divided into two groups: Experimental, treated with bacterial cellulose biopolymer film and gel, and Control, treated with essential fatty acids. Patients attended weekly appointments to change dressings and had their wound healing processes evaluated over a period of 90 days. RESULTS: the reduction of the ischemic wounds' areas after 30 days was 4.3cm2 (55%) on average for the experimental group, and the 5.5cm2 (48.5%) for the control group (p>0.05). The complete healing rate at 90 days was 34.8%, 50% in the experimental group and 18.2% in the control group (p=0.053). CONCLUSION: the bacterial cellulose biopolymer film associated with gel can be used as a dressing in the treatment of ischemic wounds of patients undergoing revascularization of the lower limbs.


Assuntos
Bandagens , Biopolímeros/uso terapêutico , Celulose/uso terapêutico , Isquemia/complicações , Isquemia/terapia , Extremidade Inferior/patologia , Cicatrização/efeitos dos fármacos , Idoso , Idoso de 80 Anos ou mais , Angioplastia , Feminino , Géis/uso terapêutico , Humanos , Isquemia/patologia , Extremidade Inferior/irrigação sanguínea , Masculino , Pessoa de Meia-Idade , Fatores de Tempo , Resultado do Tratamento
14.
Prion ; 13(1): 185-196, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-31578923

RESUMO

Prion diseases are fatal transmissible neurodegenerative disorders that affect animals and humans. Prions are proteinaceous infectious particles consisting of a misfolded isoform of the cellular prion protein PrPC, termed PrPSc. PrPSc accumulates in infected neurons due to partial resistance to proteolytic digestion. Using compounds that interfere with the production of PrPSc or enhance its degradation cure prion infection in vitro, but most drugs failed when used to treat prion-infected rodents. In order to synergize the effect of anti-prion drugs, we combined drugs interfering with the generation of PrPSc with compounds inducing PrPSc degradation. Here, we tested autophagy stimulators (rapamycin or AR12) and cellulose ether compounds (TC-5RW or 60SH-50) either as single or combination treatment of mice infected with RML prions. Single drug treatments significantly extended the survival compared to the untreated group. As anticipated, also all the combination therapy groups showed extended survival compared to the untreated group, but no combination treatment showed superior effects to 60SH-50 or TC-5RW treatment alone. Unexpectedly, we later found that combining autophagy stimulator and cellulose ether treatment in cultured neuronal cells mitigated the pro-autophagic activity of AR12 and rapamycin, which can in part explain the in vivo results. Overall, we show that it is critical to exclude antagonizing drug effects when attempting combination therapy. In addition, we identified AR-12 as a pro-autophagic drug that significantly extends survival of prion-infected mice, has no adverse side effects on the animals used in this study, and can be useful in future studies.


Assuntos
Autofagia/efeitos dos fármacos , Celulose/uso terapêutico , Proteínas PrPSc/metabolismo , Doenças Priônicas/tratamento farmacológico , Sirolimo/uso terapêutico , Animais , Celulose/análogos & derivados , Sinergismo Farmacológico , Éteres/química , Éteres/uso terapêutico , Feminino , Camundongos , Proteínas PrPSc/antagonistas & inibidores , Doenças Priônicas/metabolismo , Proteólise/efeitos dos fármacos
15.
J Wound Care ; 28(Sup10): S4-S9, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31600103

RESUMO

OBJECTIVE: To evaluate the efficacy of a biocellulose, a carboxymethyl cellulose and a normal saline wound dressing in the wound care management of epidermolysis bullosa (EB) skin wounds. METHODS: This was a single-blind, randomised controlled trial involving wounds from patients with EB. Wounds were divided into three groups: group I with biocellulose wound dressing, group II with carboxymethyl cellulose wound dressing and group III with normal saline wound dressing as a control. All dressing changes and wound parameters were recorded. Observations were conducted every three days until complete wound closure or up to one month. RESULTS: The outcomes of treatment of 36 wounds from four patients were evaluated in this study. Mean healing time in group I was seven days, eight days in group II and 14 days in group III. There were significant differences in healing times between group I and group III (p=0.0001) and between group II and III (p=0.001). The results showed a significant reduction in the percentage of wounds area on day three for each group: 51.7% in group I, 51.9% in group II, and 26% for group III. All wounds in groups I and II had healed at day 12 (100%) and at day 24 (100%) in group III. There were significant differences in the reduction of percentage wound area between group I and group III at day three (p=0.044) and day six (p=0.000), and between group II and III at day six (p=0.003). CONCLUSION: The study demonstrates that both the biocellulose and the carboxymethyl cellulose wound dressings significantly reduced percentage wound areas and complete healing times compared with the normal saline wound dressing in EB skin wounds, demonstrating they are both equally good for wound care management in EB patients.


Assuntos
Bandagens , Carboximetilcelulose Sódica/uso terapêutico , Celulose/uso terapêutico , Epidermólise Bolhosa/terapia , Solução Salina/uso terapêutico , Cicatrização/efeitos dos fármacos , Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Humanos , Indonésia , Lactente , Masculino , Método Simples-Cego , Resultado do Tratamento , Adulto Jovem
16.
Prensa méd. argent ; 105(9 especial): 521-525, oct 2019. tab
Artigo em Inglês | LILACS, BINACIS | ID: biblio-1046371

RESUMO

Over the last decade, the interest in using bacterial cellulose in medicine has increased. The article publishes the data about the efficiency of healing burn wounds in rabbits in experimental conditions with the use of the DermaRM wound dressing, compared to the traditionally used Panthenol ointment and the Branolind N salve dressing


Assuntos
Animais , Coelhos , Pomadas/uso terapêutico , Bandagens , Queimaduras/terapia , Celulose/uso terapêutico , Resultado do Tratamento , Tempo para o Tratamento
17.
Biochem Biophys Res Commun ; 516(4): 1167-1174, 2019 09 03.
Artigo em Inglês | MEDLINE | ID: mdl-31284954

RESUMO

Promotion of wound healing is one of the most important fields in clinical medical research. This study aimed to evaluate the potential use of a new surface-structured bacterial cellulose(S-BC) biomaterial with human urine-derived stem cells (hUSCs) for wound healing. In vitro, EA.hy926 were inoculated on structured/non-structured bacterial cellulose, and the growth of EA.hy926 on bacterial cellulose in medium with/without conditioned medium of the hUSCs were observed to explore the effect of bacterial cellulose's surface structure and hUSCs-CM on vascular endothelial cell growth. In vivo, we covered wound surface with various BC materials and/or injected the hUSCs into the wound site on group BC, group S-BC, group hUSCs, group BC + hUSCs, group S-BC + hUSCs to evaluate the effect of S-BC and hUSCs on wound healing in rat full-thickness skin defect model. In vitro study, surface structure of S-BC could promote the growth and survival of EA.hy926, and the hUSCs-CM could further promote the proliferation of EA.hy926 on S-BC. In vivo study, wound healing rate of the group BC, group S-BC, group hUSCs was significantly accelerated, accompanied by faster re-epithelialization, collagen production and neovascularization than control group. It is note worthy that the effect of S-BC on wound healing was better than BC, the effect of S-BC + hUSCs on wound healing was better than BC + hUSCs. Moreover, the effect of S-BC combined with hUSCs on wound is better than treated with S-BC or hUSCs alone. All the findings suggest that the combination of S-BC and hUSCs could facilitate skin wound healing by promoting angiogenesis. This combination of the role of stem cells and biomaterial surface structures may provide a new way to address clinical wound healing problems.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Celulose/uso terapêutico , Neovascularização Fisiológica , Transplante de Células-Tronco , Cicatrização , Animais , Materiais Biocompatíveis/química , Linhagem Celular , Células Cultivadas , Celulose/química , Células Endoteliais/citologia , Humanos , Polissacarídeos Bacterianos/química , Polissacarídeos Bacterianos/uso terapêutico , Ratos , Transplante de Células-Tronco/métodos , Células-Tronco/citologia , Propriedades de Superfície , Tecidos Suporte/química
18.
Carbohydr Polym ; 219: 210-218, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31151519

RESUMO

Scaffold plays a critical role in stem cell differentiation and tissue regeneration. Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study. The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%. They were also favorable with cell attachment and growth. After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold. When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1). The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.


Assuntos
Celulose/química , Colágeno/química , Gluconacetobacter xylinus/metabolismo , Engenharia Tecidual/métodos , Tecidos Suporte/química , Animais , Regeneração Óssea/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Celulose/uso terapêutico , Colágeno/uso terapêutico , Humanos , Células-Tronco Mesenquimais/efeitos dos fármacos , Camundongos , Células NIH 3T3 , Osteogênese/efeitos dos fármacos
19.
Biomed Pharmacother ; 112: 108640, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30784929

RESUMO

Since early times, propolis has been used in folk medicine. The red propolis, collected in the northeast region of Brazil has been highlighted due to its popular use as an antimicrobial, with anti-inflammatory and healing properties, which are associated with its chemical composition. Here, we combine a bacterial membrane with red propolis to treat wounds of diabetic mice. This work aims to evaluate a biocurative from bacterial cellulose associated with red propolis in diabetic mice as wound healing model. Biocuratives from bacterial cellulose membrane and different extracts of red propolis were produced. The qualification and quantification of the presence of propolis chemical compounds in the membrane were investigated through high-resolution mass spectrometry (HRMS). Tests in vivo with biocuratives were performed on Swiss male diabetic mice induced by estroptozotocin. The animals were submitted to a surgical procedure and a single lesion was produced in the dorsal region, which was treated with the biocuratives. Macroscopic assessments were performed at 2, 7 and 14 postoperative days, and biopsies were collected on days 0, 7 and 14 for histological analysis, myeloperoxidase enzyme activity (MPO) and cytokines (TNF-α, IL-1ß, and TGF-ß). Altogether, ten compounds were identified in membranes and five were further quantified. The ethyl acetate extract showed more red propolis markers, and the most prevalent compound was Formononetin with 4423.00-2907.00 µg.g-1. Macroscopic analyses demonstrated that the two groups treated with red propolis (GMEBT and GMEAE) showed significantly greater healing capabilities compared to the control groups (GS and GMS). An increase in leukocyte recruitment was observed, confirmed by the activity of the enzyme myeloperoxidase (MPO) in GMEBT and GMEAE groups. The levels of TNF-α were significantly higher in wounds stimulated with red propolis, as well as in TGF-ß (GMEBT and GMEAE) on day 7. This was different from the IL-1ß levels that were higher in the control groups (GS and GMS). In summary, the biocuratives produced in this work were able to accelerate the wound healing process in a diabetic mouse model. In this way, the traditional knowledge of red propolis activity helped to create a biotechnological product, which can be used for diabetic wound healing purpose.


Assuntos
Acetobacteraceae/química , Celulose/uso terapêutico , Diabetes Mellitus Experimental/tratamento farmacológico , Própole/uso terapêutico , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Animais , Membrana Celular/química , Celulose/administração & dosagem , Celulose/isolamento & purificação , Diabetes Mellitus Experimental/complicações , Masculino , Própole/administração & dosagem , Pele/lesões , Resultado do Tratamento
20.
Int J Biol Macromol ; 127: 606-617, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30685305

RESUMO

The use of cellulose as an alternative to synthetic polymers might permit the decrease of environmental impacts by achieving more easily biodegradable or reusable materials. Recently, cellulose and inorganics are synergistically coupled to tailor promising functional hybrids with unique properties. The current review presents an overview of the recent trials for preparing cellulose/calcium phosphate hybrid materials and offers prospects for many new applications in diverse fields. During the whole review, different cellulose forms and cellulose derivatives are being explored as fillers or templates for the design of new functional materials and support for the loading of different calcium phosphate phases. The functionalization of cellulose with calcium phosphate was carried out to construct new and attractive physicochemical properties that are being exploited for different applications such as bone regeneration, drug delivery vehicles, dental repair and adsorption. The studying of cellulose/calcium phosphate hybrid materials is supported by increasing concern in providing smart materials that combine best of the calcium phosphate properties along with cellulose. The review also aims to study the driving forces and limitations towards structuring of cellulose/calcium phosphate hybrid materials. It appears that the use of cellulosic materials for constructing new hybrids is very promising for biomedical and environmental applications.


Assuntos
Fosfatos de Cálcio , Celulose , Portadores de Fármacos , Animais , Regeneração Óssea/efeitos dos fármacos , Fosfatos de Cálcio/química , Fosfatos de Cálcio/uso terapêutico , Celulose/química , Celulose/uso terapêutico , Portadores de Fármacos/química , Portadores de Fármacos/uso terapêutico , Humanos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...