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1.
J Orthop Surg Res ; 16(1): 356, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-34074300

RESUMO

BACKGROUND: The coronavirus disease (COVID-19) pandemic has had a massive impact on individuals globally. The Chinese government has formulated effective response measures, and medical personnel have been actively responding to challenges associated with the epidemic prevention and control strategies. This study aimed to evaluate the effect of the implementation of a care transition pathway on patients that underwent joint replacement during the COVID-19 pandemic. METHODS: A quasi-experimental study was designed to evaluate the effect of implementing a care transition pathway for patients who underwent joint replacement during the COVID-19 pandemic in the orthopedic department of a tertiary care hospital in Beijing, China. Using a convenient sampling method, a total of 96 patients were selected. Of these, 51 patients who had undergone joint replacement in 2019 and received treatment via the routine nursing path were included in the control group. The remaining 45 patients who underwent joint replacement during the COVID-19 epidemic in 2020 and received therapy via the care transition pathway due to the implementation of epidemic prevention and control measures were included in the observation group. The quality of care transition was assessed by the Care Transition Measure (CTM), and patients were followed up 1 week after discharge. RESULTS: The observation group was determined to have better general self-care preparation, written planning materials, doctor-patient communication, health monitoring, and quality of care transition than the control group. CONCLUSIONS: A care transition pathway was developed to provide patients with care while transitioning through periods of treatment. It improved the patient perceptions of nursing quality. The COVID-19 pandemic is a huge challenge for health professionals, but we have the ability to improve features of workflows to provide the best possible patient care.


Assuntos
Artroplastia de Substituição/tendências , 59585/epidemiologia , Ensaios Clínicos Controlados não Aleatórios como Assunto/tendências , Procedimentos Ortopédicos/tendências , Centros de Atenção Terciária/tendências , Cuidado Transicional/tendências , Idoso , Artroplastia de Substituição/métodos , Artroplastia de Substituição/reabilitação , Pequim/epidemiologia , 59585/prevenção & controle , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ensaios Clínicos Controlados não Aleatórios como Assunto/métodos , Procedimentos Ortopédicos/métodos , Procedimentos Ortopédicos/reabilitação , Pandemias , Resultado do Tratamento
2.
Wei Sheng Yan Jiu ; 50(3): 377-381, 2021 May.
Artigo em Chinês | MEDLINE | ID: mdl-34074359

RESUMO

OBJECTIVE: To describe the hemoglobin and prevalence of anemia among 6-23 months infants at different elevations in poor rural areas in China. METHODS: The monitoring data in the year 2018 from the Children Nutrition Improvement Project in Poor Areas of China was used for the analysis, which covered 19 provinces and 42 938 infants of 6-23 months. Hemoglobin was tested by Hemoque method with blood drop from finger tips. The altitude of the residence regions was divided into eight parts. Analysis of variance was used to compare the mean values among the altitude groups, and chi-square test was used to compare the prevalence of anaemia. RESULTS: The data from 42 938 6-23 months infants was under analysis, among them 22 207(51.7%)were boys and 20 731(48.4%)girls. 27 208(63.4%)infants and children were located in the areas under 1000 meters whose average hemoglobin was 11.8 g/dL and the prevalence of anemia was 18.7%. There were 15 730(36.6%)infants living in areas higher than 1000 meters. The anemia rate ranged from 31.3% to 83.6% in all aged groups when altitude& gt; 2500 m that were noticeable higher than that of lower altitude regions. With the increase of altitude, the adjusted anemic rate showed an obvious upward trend. 6-11 months infants showed higher anemia rate compared with other groups. CONCLUSION: The incidence of anemia among children aged 6 to 23 months in poor areas of China is severe, among which infants living at an altitude of more than 2500 meters have a higher prevalence of anemia.


Assuntos
Altitude , Anemia , Pobreza , Anemia/epidemiologia , China/epidemiologia , Feminino , Hemoglobinas/análise , Humanos , Lactente , Masculino , Prevalência , População Rural
3.
Wei Sheng Yan Jiu ; 50(3): 460-465, 2021 May.
Artigo em Chinês | MEDLINE | ID: mdl-34074369

RESUMO

OBJECTIVE: To explore the relationship between dietary inflammatory index(DII), serum interleukin-6(IL-6) of late pregnant women and infant birth weight. METHODS: This study was conducted in late pregnant women who received antenatal care at the Maternal and Children& apos; s Hospital of Baiyun and Yuexiu District in Guangzhou, China between September 2010 and February 2011. Tree consecutive 24-h diet recalls, pre-pregnancy body mass index, maternal education level and etc were collected. DII score was calculated based on data of dietary surveys. Participant were dived into anti-inflammatory group(T1), neutral group(T2) and pro-inflammatory group(T3) according to the tertiles of DII score. Maternal and infant anthropometric profile(n=456) and level of maternal serum IL-6(n=308) were measured. Structrural equation modeling(SEM) was used to explore the relationship between DII, IL-6 and birth weight. RESULTS: In 456 women, the mean DII score was 0. 02±1. 08. Women performed anti-inflammatory diet had higher intake of grain and potato, vegetables, energy, fiber, minerals, vitamins and unsaturated fatty acids, but lower intakes of fruits, diary, fat(% energy) and protein from animal food(P& lt; 0. 05). Average infant birth weight and level of maternal serum IL-6 were(3238. 1±376. 4)g and 4. 05(2. 02, 10. 14) pg/mL respectively. DII of pregnant women was positively correlated with maternal serum IL-6(r=0. 144, P& lt; 0. 05), and IL-6 was negatively correlated with birth weight(r=-0. 184, P& lt; 0. 05). SEM indicated that maternal serum IL-6 may be a mediator in the association between DII and birth weight. CONCLUSION: The diet of pregnant women may change the serum level of IL6, and then affect infant birth weight.


Assuntos
Peso ao Nascer , Dieta , Interleucina-6 , Gestantes , China , Feminino , Humanos , Recém-Nascido , Inflamação , Interleucina-6/sangue , Gravidez
4.
Sci Rep ; 11(1): 11462, 2021 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-34075090

RESUMO

An excessive immune response known as cytokine storm is the hallmark of severe COVID-19. The cause of this cytokine rampage is yet not known. Based on recent epidemiological evidence, we hypothesized that CD80/86 signaling is essential for this hyperinflammation, and that blocking this proinflammatory axis could be an effective therapeutic approach to protect against severe COVID-19. Here we provide exploratory evidence that abatacept, a drug that blocks CD80/86 co-stimulation, produces changes at the systemic level that are highly antagonistic of the proinflammatory processes elicited by COVID-19. Using RNA-seq from blood samples from a longitudinal cohort of n = 38 rheumatic patients treated with abatacept, we determined the immunological processes that are significantly regulated by this treatment. We then analyzed available blood RNA-seq from two COVID19 patient cohorts, a very early cohort from the epicenter of the pandemic in China (n = 3 COVID-19 cases and n = 3 controls), and a recent and larger cohort from the USA (n = 49 severe and n = 51 mild COVD-19 patients). We found a highly significant antagonism between SARS-CoV-2 infection and COVID-19 severity with the systemic response to abatacept. Analysis of previous single-cell RNA-seq data from bronchoalveolar lavage fluid from mild and severe COVID-19 patients and controls, reinforce the implication of the CD80/86 proinflammatory axis. Our functional results further support abatacept as a candidate therapeutic approach to prevent severe COVID-19.


Assuntos
Abatacepte/farmacologia , 59585/tratamento farmacológico , Síndrome da Liberação de Citocina/prevenção & controle , Imunossupressores/farmacologia , 59565/imunologia , Transdução de Sinais/efeitos dos fármacos , Abatacepte/uso terapêutico , Idoso , Artrite Reumatoide/sangue , Artrite Reumatoide/tratamento farmacológico , Artrite Reumatoide/imunologia , Antígeno B7-1/metabolismo , Antígeno B7-2/metabolismo , Líquido da Lavagem Broncoalveolar/citologia , 59585/sangue , 59585/complicações , 59585/imunologia , China , Síndrome da Liberação de Citocina/imunologia , Síndrome da Liberação de Citocina/virologia , Feminino , Humanos , Imunossupressores/uso terapêutico , Masculino , Pessoa de Meia-Idade , Estudos Observacionais como Assunto , RNA-Seq , Índice de Gravidade de Doença , Transdução de Sinais/imunologia , Análise de Célula Única , Espanha , Estados Unidos , Regulação para Cima/efeitos dos fármacos , Regulação para Cima/imunologia
5.
Wiad Lek ; 74(5): 1152-1157, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34090282

RESUMO

OBJECTIVE: The aim: Optimizing the interdisciplinary approaches in the diagnosis and monitoring the dynamics of uterine leiomyoma treatment by high-intensity focused ultrasound ablation. PATIENTS AND METHODS: Materials and methods: In the course of scientific research we conducted a survey 72 women of reproductive age were diagnosed with leiomyoma. All patients underwent bimanual gynecological examination, ultrasound and MRI to determine the condition of the pelvic organs, assess the structure, location, number of myomas, as well as assess the possible acoustic pathway of high-intensity focused ultrasound. During monitoring, dynamic contrasting was used to determine the zone of node necrosis. Methods of control in the postoperative period: ultrasound, MRI of the pelvic organs using paramagnetic, were performed after 1, 3, 6 months. Ultrasound ablation of uterine fibroids was performed using the JC extracorporeal treatment system (Chongqing HAIFU (HIFU) Technology Co. Ltd., China) with a built-in ultrasound system (Italy). Controlling the direct result was based on gray scale changes during real-time ultrasound examination on the monitor of the JC device. Since HIFU is a non-invasive method of treatment, in the future, the diagnosis was limited to using the ultrasound and MRI paramagnetic. Indicators such as: uterine body size were considered as indicators that characterize the effectiveness of treatment; specific volume of myoma; regression of uterine body size; regression of the myoma; regression of the node, calculated on its specific volume, because one patient could have several nodes. RESULTS: Results: Analyzing the obtained results, it should be noted that for a month the average volume of leiomatous hives that were exposed to HIFU, almost did not change and was 122 cm³, while three months after treatment it was - 98 cm. The nodes underwent a significant reduction 6 months after the treatment, their volume averaged 61 cm³. The dynamics analysis results of uterine body volume reduction, which is no less important expected result, showed the following: in the first month after the intervention the uterine volume almost did not change and, compared to the average size before treatment 342cm³, was 300cm³. In three months after treatment, the body volume of the uterus decreased to 264 cm³, and in six months - to 200 cm³. When assessing the node 6 months after the procedure, it was found that during this period there was a significant reduction in the volume of leiomyoma, which was positively correlated with the clinical manifestations of the disease. CONCLUSION: Conclusions: An interdisciplinary approach with the widespread introduction of modern organ-preserving techniques is an important direction in maintaining the reproductive health of women with uterine leiomyoma. Positive dynamics of leiomatous node volume regression depends on its location, volume and MR type. The terms 1 and 3 months after ultrasound ablation are insufficient for objective radiological evaluation of the treatment outcome, but are important for the choice of further tactics in observing and treating the uterine leiomyoma.


Assuntos
Saúde Reprodutiva , Neoplasias Uterinas , China , Feminino , Humanos , Itália , Neoplasias Uterinas/diagnóstico por imagem , Neoplasias Uterinas/cirurgia
6.
BMC Surg ; 21(1): 284, 2021 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-34090388

RESUMO

BACKGROUND: Full-thickness rectal prolapse (FTRP) frequently occurs in elderly women, and more than 100 surgical procedures have been proposed to restore FTRP. The Gant-Miwa-Thiersch (GMT) procedure is the most used treatment in China. However, the recurrence rate of FTRP post-GMT, which is as high as 23.8%, is concerning. We described a new modified GMT combined with internal and external rectal sclerosant injection (nmGMTSI) procedure to address this problem. METHODS: The nmGMTSI was performed under spinal anesthesia in 34 frail, elderly female patients with FTRP. The surgical results of FTRP were assessed. Fecal incontinence and constipation were evaluated using the Wexner score, and anal canal rest pressure (ACRP), maximum anal systolic pressure (MASP), anorectal sensation thresholds (AST), and maximum rectal tolerance (MRT) using anorectal manometry preoperatively and postoperatively. The causes of recurrence and complications were analyzed. RESULTS: All patients were cured according to the clinical cure standard. The perioperative Wexner fecal incontinence score (WFIS) was 10.3 ± 3.31, which became 3.7 ± 2.43 (P < 0.0001) postoperatively. The perioperative ACRP was 2.0 ± 0.56 kPa, which became 8.5 ± 2.25 kPa (P < 0.0001) postoperatively. The perioperative MASP was 4.5 ± 1.16 kPa, which became 18.6 ± 2.50 kPa (P < 0.0001) postoperatively. However, no significant difference was observed between the preoperative and postoperative Wexner constipation scores (WCS) (17.3 ± 2.25 vs. 15.4 ± 2.89, P = 0.1047). The perioperative and postoperative AST were 38.1 ± 5.34 mL and 23.5 ± 3.61 mL, respectively (P = 0.0002). The maximum rectal tolerance (MRT) was 157.1 ± 16.73 mL, which became 121.2 ± 12.45 mL postoperatively (P = 0.0009). The patients developed no serious postoperative complications. The total relapse rate after nmGMTSI was 2.9% in the median two years follow-up period. The most common cause of relapse after nmGMTSI was the removal of infected threads used in the Thiersch procedure. CONCLUSION: The benefits of nmGMTSI include low rates of recurrence, complications, and mortality, cost-effectiveness, wide adaptation, minimal invasiveness, and technical simplicity. Hence, it should be considered the first option for the treatment of FTRP in frail elderly women.


Assuntos
Prolapso Retal , Soluções Esclerosantes , Idoso , China , Feminino , Humanos , Prolapso Retal/cirurgia , Reto/cirurgia , Escleroterapia
7.
World J Surg Oncol ; 19(1): 163, 2021 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-34090483

RESUMO

BACKGROUND: The incidence of gallbladder carcinoma (GBM) in China has increased in recent years. Here, the functional mechanism of lncRNA TTN-AS1 in GBC was preliminary elucidated. METHODS: The expression levels of lncRNA TTN-AS1, miR-107, and HMGA1 in tissues and cell lines were assessed by RT-qPCR. Cell proliferation was measured by MTT assays. Cell invasion and migration abilities were evaluated by Transwell assays. The relationship between miR-107 and lncRNA TTN-AS1 or HMGA1 was confirmed by luciferase reporter assay. RESULTS: Upregulation of lncRNA TTN-AS1 and downregulation of miR-107 were detected in GBC. Furthermore, the expressions between TTN-AS1 and miR-107 were mutually inhibited in GBC. Functionally, lncRNA TTN-AS1 promoted cell viability and motility in GBC by sponging miR-107. In addition, miR-107 directly targets HMGA1. And HMGA1 can be positively regulated by lncRNA TTN-AS1 in GBC. Furthermore, HMGA1 promoted GBC progression by interacting with lncRNA TTN-AS1/miR-107 axis. CONCLUSION: LncRNA TTN-AS1 acted as a tumor promoter in GBC by sponging miR-107 and upregulating HMGA1.


Assuntos
Neoplasias da Vesícula Biliar , MicroRNAs , RNA Longo não Codificante , Carcinógenos , Linhagem Celular Tumoral , China , Neoplasias da Vesícula Biliar/genética , Regulação Neoplásica da Expressão Gênica , Proteína HMGA1a/genética , Humanos , MicroRNAs/genética , Prognóstico , RNA Longo não Codificante/genética
8.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 38(6): 513-520, 2021 Jun 10.
Artigo em Chinês | MEDLINE | ID: mdl-34096016

RESUMO

The use of whole exome sequencing (WES) for the detection of disease-causing variants of genetic diseases and for non-invasive prenatal screening (NIPS) of fetal aneuploidies are two major clinical applications of next generation sequencing (NGS). This article has summarized the official documents developed and updated by the American College of Medical Genetics and Genomics (ACMG) on governing WES and NIPS. These include the development of expert consensus policies and position statements on an ongoing basis to guide clinical application of NGS technology and variant analysis, establish evidence-based practical resources, as well as standards and guidelines to govern diagnosis and screening. These ACMG documents are valuable references to Chinese geneticists, but direct adoption of these standards and guidelines may not be practical due to the differences in disease-associated variant frequencies in Chinese population, socioeconomic status, and medical practice between the two countries. It is hoped that this review could facilitate the development of NGS and NIPS standards and guidelines that are consistent with international standards and concordant with medical genetics practice in China to provide high-quality, efficient and safe clinical services for patients and their families with genetic diseases.


Assuntos
Genômica , Sequenciamento de Nucleotídeos em Larga Escala , China , Consenso , Feminino , Humanos , Gravidez , Tecnologia , Estados Unidos
9.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 38(6): 557-560, 2021 Jun 10.
Artigo em Chinês | MEDLINE | ID: mdl-34096025

RESUMO

OBJECTIVE: To carry out genetic testing for a Chinese patient with X-linked hypohidrotic ectodermal dysplasia (XLHED) and explore its genotype-phenotype correlation. METHODS: Clinical data of the patient was collected. Peripheral blood samples were taken from the patient, his parents and 100 unrelated healthy controls. Genetic variants were detected by using next-generation sequencing using a skin-disease panel through targeted capture and next generation sequencing. Candidate variant was verified by Sanger sequencing. All literature related to genetic testing of XLHED patients in China was searched in the database, and the genotypes and phenotypes of patients in the literature and the correlation between them were statistically analyzed. RESULTS: A novel splice site variant c.655_689del was detected in the patient but not among his parents and the 100 unrelated healthy controls. So far 61 variants of the EDA gene have been identified among Chinese patients with XLHED, which suggested certain degree of genotype-phenotype correlation. CONCLUSION: A novel c.655_689del variant has been identified in the EDA gene, which has expanded the spectrum of EDA gene variant and facilitated delineation of the genotype-phenotype correlation of XLHED.


Assuntos
Displasia Ectodérmica Anidrótica Tipo 1 , Criança , China , Displasia Ectodérmica Anidrótica Tipo 1/genética , Ectodisplasinas/genética , Testes Genéticos , Genótipo , Humanos , Fenótipo
10.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 38(6): 589-592, 2021 Jun 10.
Artigo em Chinês | MEDLINE | ID: mdl-34096033

RESUMO

OBJECTIVE: To study the distribution of KIR3DL2 alleles among ethnic Han Chinese from Zhejiang. METHODS: Genomic DNA was extracted by using a magnetic bead method. The full sequence of the KIR3DL2 gene was amplified with four pairs by PCR primers. The coding regions of 208 unrelated ethnic Han Chinese blood donors were analyzed using a BigDye Terminator v3.1 Sequencing Kit. The genotypes were assigned based on the nucleotide polymorphism of the KIR3DL2 gene. RESULTS: Among the 208 samples, 133 were KIR3DL2 heterozygotes and 75 were homozygotes. Forty six KIR3DL2 genotypes were detected. Respectively, 70, 33 and 23 individuals were found to have a KIR3DL2*00201/KIR3DL2*00201, KIR3DL2*00201/KIR3DL2*00701, and KIR3DL2*00201/KIR3DL2*01001 genotype. Twenty-two KIR3DL2 alleles were discovered, and the frequencies of KIR3DL2*00201, KIR3DL2*00701 and KIR3DL2*01001 were 57.45%, 13.46% and 9.13%, respectively. CONCLUSION: The distribution of KIR3DL2 alleles among ethnic Han Chinese in Zhejiang has been determined and fits the criteria for genetic polymorphism.


Assuntos
Grupos Étnicos , Polimorfismo Genético , Alelos , China , Frequência do Gene , Humanos , Receptores KIR3DL2
11.
Zhongguo Yi Liao Qi Xie Za Zhi ; 45(3): 261-265, 2021 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-34096232

RESUMO

Based on the clinical application data of medical X-ray computed tomography (CT) in the Shanghai Sixth People's Hospital, this study transformed it into the product reliability index requirements, and took the mechanical representative component-examination table (hereinafter referred to as "patient table") and the electronic representative component-DCB (data control board) as examples. Based on the relationship between failure characteristics and clinical application data, a complete set of closed-loop implementation methods from reliability index requirements to reliability design and verification are discussed.


Assuntos
Tomografia Computadorizada por Raios X , China , Humanos , Reprodutibilidade dos Testes
12.
Zhongguo Yi Liao Qi Xie Za Zhi ; 45(3): 315-320, 2021 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-34096244

RESUMO

OBJECTIVE: Discuss the working ideas of the dynamic adjustment mechanism of medical device classification in the United States, and provide reference for the construction of medical device related mechanisms in China. METHODS: Collect and interpret the documents of regulatory background, procedures and orders of the dynamic adjustment mechanism of the medical device classification in the United States, and summarize the overall situation and specific cases of the medical device classification adjustment under this mechanism in recent years. RESULTS: The US work idea of the medical device classification dynamic adjustment mechanism is based on the latest valid scientific evidence, conducting risk analysis and identification, and determining the corresponding measures. CONCLUSIONS: During the adjustment process, industry stakeholders have repeatedly discussed and achieved final agreement. Its procedures and working ideas can be used as a reference for China's work.


Assuntos
United States Food and Drug Administration , China , Estados Unidos
13.
Environ Monit Assess ; 193(7): 381, 2021 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-34085125

RESUMO

Cadmium (Cd) can be leached from soil into the groundwater and exhibit its adverse effect on the health of animals and humans. While previous studies have studied the process of Cd transport in water-saturated sand columns, literature regarding Cd transport in soil is scarce. The aim of this experiment was to investigate the transport of Cd in soil columns and biochar application rate effects on the mobility and distribution of Cd in soil. The red paddy soil was collected from the paddy of Changsha County, Hunan Province in southern China. Batch sorption and column experiments were conducted to study the adsorption isotherms of Cd2+ and its mobility at different biochar application rate treatments (0, 0.5, 1, 1.5, and 2%) referenced here as A0, A10, A20, A30, and A40, respectively. The Cd concentration of in effluent samples and digestion solutions was measured by inductively coupled plasma optical emission spectrometer (ICP-OES, Thermo Fisher Scientific, USA). After finishing the column experiment, columns were dissected into five layers (1-cm segments), the Cd fractions in soil were performed by the European Community Bureau of Reference (BCR). The amount of Cd sorption among treatments decreased in the order of A40 > A30 > A20 > A10 > A0, and the Langmuir model was more suitable to study the Cd2+ adsorption on biochar-amended soil than Freundlich model. Breakthrough curves showed that increasing biochar application rate increased the initial breakthrough time, whereas the pore-water velocity and dispersion coefficient were 81.0 and 99.8% lower in the A40 treatments than in the A0 treatments, respectively. Increasing biochar application rate enhanced the pH but reduced redox potential (Eh) in the most of effluents. Compared with A0, the concentration of Cd retained in soil columns increased by 86.6% in the A40 treatments. However, BCR sequential extractions showed that biochar addition in A40 treatments increased the acid soluble fraction but reduced the reducible fraction. In A40 treatments, compared with the 0-1-cm soil layer, the relative Cd concentration (N/Ni) in the 1-2-, 2-3-, 3-4-, and 4-5-cm soil layers increased by 5.4, 10.9, 14.3, and 21.9%, respectively. Biochar application in A40 treatments showed strong capacity for retarding Cd transport in soil, while the potential mobility of Cd in soil should be considered.


Assuntos
Poluentes do Solo , Solo , Animais , Cádmio/análise , Carvão Vegetal , China , Monitoramento Ambiental , Humanos , Poluentes do Solo/análise , Triticum
14.
Sheng Wu Gong Cheng Xue Bao ; 37(5): 1677-1696, 2021 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-34085449

RESUMO

Fermentative production of amino acids is one of the pillars of the fermentation industry in China. Recently, with the fast development of metabolic engineering and synthetic biology technologies, the metabolic engineering for production of amino acids has been flourishing. Conventional forward metabolic engineering, reversed metabolic engineering based on omics data and in silico simulation, and evolutionary metabolic engineering mimicking the natural evolution, have shown increasingly promising applications. A series of highly efficient and robust amino acids-producing strains have been developed and applied in the industrial production of amino acids. The increasingly fierce market competition has put forward new requirements for strain breeding and selection, such as developing high value-added amino acids, dynamic regulation of cellular metabolism, and adapting to the requirements of new process. This review summarizes the advances and prospects in metabolic engineering for the production of amino acids.


Assuntos
Corynebacterium glutamicum , Engenharia Metabólica , Aminoácidos , China , Corynebacterium glutamicum/genética , Biologia Sintética
15.
Zhen Ci Yan Jiu ; 46(5): 426-30, 2021 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-34085468

RESUMO

Master DOU Han-qing is one famous acupuncturist in the Jin and Yuan Dynasties of China. In consideration of his newly neatened works DOU Taishi Zhenjing (Master DOU's Canon of Acupuncture,《》), Zhenjiu Jicheng (Compendium of Acupuncture and Moxibustion,《》) and Panshijin Zhici Michuan (Panshijin Secretly Bequeathed Techniques of Straight Needling,《》) which were not paid enough attention in the past, we make a systematic and comprehensive summary about his academic achievements in the present paper. There are six main aspects about Dou's academic achievements: blending the essence of sages' acupuncture theories and clinical experience, correcting predecessors' mistakes; innovating the needlingmethods; expanding the clinical applications of acupoints and paying attention to combination of meridian acupoints and extra-points in clinical practice; inheriting and developing acupuncture prescriptions in the Song Dynasty, creating new diagnostic and treatment modes; initiating needling techniques of various schools in the Ming Dynasty; and describing the contents of acupuncture and moxibustion in the form of odes. Moreover, we also put forward some of our enlightenments and considerations about DOU Han-qing's academic achievements.


Assuntos
Sucesso Acadêmico , Terapia por Acupuntura , Acupuntura , Moxibustão , Pontos de Acupuntura , China
16.
Zhongguo Zhen Jiu ; 41(6): 691-8, 2021 Jun 12.
Artigo em Chinês | MEDLINE | ID: mdl-34085491

RESUMO

OBJECTIVE: To review systematically the effectiveness and safety of tuina (Chinese massage)in treatment of functional constipation. METHODS: The articles on functional constipation treated with tuina were collected by computer retrieval from 7 databases from the date of establishment to March 28, 2020, including Chinese biomedical literature database (SinoMed), China journal full-text database (CNKI), full-text database of Wanfang academic journals (Wanfang), VIP Chinese science and technology journal database(VIP), PubMed, Dutch medical literature database (EMbase) and the Cochrane Library. After data extraction and quality evaluation of the included articles, Meta analysis was conducted with RevMan5.3 software. RESULTS: A total of 16 articles were included, with 1424 cases involved. Meta analysis results showed: ①The total effective rate in the treatment group was higher than that in the control group (RR=1.28, 95%CI: 1.16-1.42, P<0.000 01). ②The effective rate for the symptoms of functional constipation in traditional Chinese medicine in the treatment group was higher than that in the control group (RR=1.38, 95%CI :1.25-1.52, Z=6.31, P<0.000 01). ③Adverse reactions in the treatment group in the treatment of functional constipation were less than those in the control group (RR=0.10, 95%CI: 0.02-0.49, Z=2.81, P=0.005).④The effective rate of functional constipation treated on the base of syndrome differentiation in the treatment group was higher than that of the control group (RR=1.50, 95%CI: 1.08-2.10, Z=2.39, P=0.02).⑤The improvements in fecal characteristics, defecation time and defecation frequency of the patients with functional constipation in the treatment group were better than those in the control group (P<0.05). CONCLUSION: Tuina therapy presents a certain advantages on its curative effect on functional constipation, has less adverse reactions and relieves the relevant symptoms of functional constipation. But more randomized controlled trials with high quality and large sample are required to provide further verification of its effect.


Assuntos
Constipação Intestinal , Medicina Tradicional Chinesa , China , Constipação Intestinal/terapia , Humanos , Massagem
17.
BMC Genomics ; 22(1): 401, 2021 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-34058976

RESUMO

BACKGROUND: Duck is an ancient domesticated animal with high economic value, used for its meat, eggs, and feathers. However, the origin of indigenous Chinese ducks remains elusive. To address this question, we performed whole-genome resequencing to first explore the genetic relationship among variants of these domestic ducks with their potential wild ancestors in eastern China, as well as understand how the their genomes were shaped by different natural and artificial selective pressures. RESULTS: Here, we report the resequencing of 60 ducks from Chinese spot-billed ducks (Anas zonorhyncha), mallards (Anas platyrhnchos), Fenghua ducks, Shaoxing ducks, Shanma ducks and Cherry Valley Pekin ducks of eastern China (ten from each population) at an average effective sequencing depth of ~ 6× per individual. The results of population and demographic analysis revealed a deep phylogenetic split between wild (Chinese spot-billed ducks and mallards) and domestic ducks. By applying selective sweep analysis, we identified that several candidate genes, important pathways and GO categories associated with artificial selection were functionally related to cellular adhesion, type 2 diabetes, lipid metabolism, the cell cycle, liver cell proliferation, and muscle functioning in domestic ducks. CONCLUSION: Genetic structure analysis showed a close genetic relationship of Chinese spot-billed ducks and mallards, which supported that Chinese spot-billed ducks contributed to the breeding of domestic ducks. During the long history of artificial selection, domestic ducks have developed a complex biological adaptation to captivity.


Assuntos
Diabetes Mellitus Tipo 2 , Domesticação , Animais , China , Patos/genética , Filogenia
18.
Artigo em Chinês | MEDLINE | ID: mdl-34074078

RESUMO

Objective: To understand the research status of occupational health risk assessment in recent ten years. Methods: In April 2020, the literatures related to occupational health risk assessment published by CNKI and Web of Science core collection (WoSCC) databases from 2010 to 2019 were searched, and Excel 2016 software was used to organize the literature, CiteSpace 5.6.R2 software was used for visual analysis. Results: A total of 58 Chinese literatures and 407 English literatures were included. The authors of the high frequency posts were Zhang Meibian, and Alessandro Marinaccio, and the publishing institutions were mainly the National Institute for Occupational Health and Poison Control of Chinese Center for Disease Control and Prevention and Finnish Institute Occupational Health. The Chinese journal with the most articles was Chinese Journal of Industrial Hygiene and Occupational Diseases, and the English journal was Safety Science. Chinese high-frequency keywords mainly included risk assessment, occupational health, occupational exposure. English high-frequency keywords mainly included occupational health, risk, risk factor. The prominent words in Chinese literature were occupational health, coal dust, occupational hazards, occupational health and occupational disease hazards; Risk assessment, worker, exposure, heart disease, cardiovascular disease and so on were prominent words in English literature. Conclusion: The main research keywords in the field of occupational health risk assessment at home and abroad focus on occupational health and risk assessment, but the research direction and focus are slightly different.


Assuntos
Bibliometria , Saúde do Trabalhador , China , Bases de Dados Factuais , Publicações , Medição de Risco
19.
Artigo em Chinês | MEDLINE | ID: mdl-34074091

RESUMO

Pneumoconiosis is one of the most serious and common occupational disease in China at present, there are a variety of complications, which seriously affect the disease treatment, disease progress and prognosis, but also the direct cause of death. Therefore, the prevention and treatment of various comorbidities of pneumoconiosis is a major public health problem in our country. This article outlines the epidemiological characteristics of different comorbidities of pneumoconiosis, analyze and summarize its research status and existing problems, and put forward relevant thoughts, in order to provide reference for the prevention and treatment of pneumoconiosis comorbidities.


Assuntos
Minas de Carvão , Doenças Profissionais , Pneumoconiose , China/epidemiologia , Estudos Epidemiológicos , Humanos , Pneumoconiose/epidemiologia
20.
Wei Sheng Yan Jiu ; 50(3): 360-365, 2021 May.
Artigo em Chinês | MEDLINE | ID: mdl-34074356

RESUMO

OBJECTIVE: To evaluate the effect of Yingyangbao(YYB) intervention in 6-23 months infants in six poor counties from Shanxi, Hubei and Yunnan Provinces. METHODS: Probability proportionate to size sampling method was used to extract townships and villages, and random isometric sampling was used to select monitored infants. A baseline survey was completed in 2012. Yingyangbao was delivered to all the infants in the counties through a registration system started in 2013 and then the delivering has been continuously conducted to the infants according to their age. The cross sectional sampled infants were monitored and evaluated of the effect in 2014 and 2015 respectively. Using synthetic clustering grouping method, the infants were divided into quasi-control group(YYB-NG), YYB intervention group(YYB-FG)for comparative analysis to eliminate the effect of YYB intervention. Multiple linear regression analysis were used to establish the dose-effect relationship between YYB intervention, other factors and main nutrition indices. RESULTS: A total of 4809 individuals were included in this study, among which data of 2273 individuals were included in the YYB-NG and 2536 data were included in the YYB-FG. The body length of YYB-FG and YYB-NG was 77.6 cm and 75.9 cm, respectively. Stunting rates of YYB-FG and YYB-NG were 6. 8% and 10. 3%, respectively. Hb levels and anemia rates of YYB-FG and YYB-NG were 121. 3 g/L, 117. 1 g/L and 21. 2%, 32. 1% respectively, with statistically significant difference(P& lt; 0.05). However, there was no significant difference in body weight between the groups. Multiple linear regression showed that YYB intervention has a significant quantitative relationship with body length, Hb, HAZ. The regression also implied that the influencing factor including month-age, gender, birth-height, birth-weight, minimum dietary diversity, caregiver educational background have a significant relationship with nutrition improvement. CONCLUSION: YYB intervention can effectively improve the body length, Hb and HAZ, while reduce stunting rate and anemia rate of IYC in the intervention area.


Assuntos
Suplementos Nutricionais , População Rural , China/epidemiologia , Estudos Transversais , Humanos , Lactente , Estado Nutricional
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