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1.
J Sci Food Agric ; 102(1): 167-174, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34080199

RESUMO

BACKGROUND: Nowadays a significant amount of land contaminated with toxic elements is being used for agriculture, posing a serious risk of crop contamination and toxicity. Several methodologies are being used to remediate soil contamination, including the use of amendments such as biochar. This work evaluated the effects of biochar combined with different fertirrigations (water, a conventional fertilizer solution, or a fertilizer solution with a commercial biostimulant derived from leonardite) on the availability of toxic elements and nutrients for pepper cultivated in a soil contaminated with As, Cd, Pb, and Zn. RESULTS: Irrigation with fertilizer solutions improved plant growth regardless of the biochar amendment. Biochar decreased the bioavailability of Cu and Pb in soil and the Cu content in pepper leaves. Combined with fertilization, biochar also decreased plant As and Pb content. Biochar combined with biostimulant decreased the bioavailable content of Cd in soil and its uptake by pepper plants. CONCLUSION: The use of biochar and biostimulant presented advantages for plant production in a non-suitable scenario of nutrient scarcity and contamination. © 2021 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.


Assuntos
Capsicum/metabolismo , Carvão Vegetal/química , Produção Agrícola/métodos , Fertilizantes/análise , Nutrientes/química , Poluentes do Solo/metabolismo , Adsorção , Transporte Biológico , Cádmio/química , Cádmio/metabolismo , Capsicum/química , Capsicum/crescimento & desenvolvimento , Chumbo/análise , Chumbo/química , Chumbo/metabolismo , Nutrientes/metabolismo , Folhas de Planta/química , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Solo/química , Poluentes do Solo/química , Zinco/química , Zinco/metabolismo
2.
Molecules ; 26(13)2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34209142

RESUMO

The objective of the study was to gather insight into the metabolism of lead-removing microorganisms, coupled with Pb(II) removal, biomass viability and nitrate concentrations for Pb(II) bioremoval using an industrially obtained microbial consortium. The consortium used for study has proven to be highly effective at removing aqueous Pb(II) from solution. Anaerobic batch experiments were conducted with Luria-Bertani broth as rich growth medium over a period of 33 h, comparing a lower concentration of Pb(II) with a higher concentration at two different nutrient concentrations. Metabolite profiling and quantification were conducted with the aid of both liquid chromatography coupled with tandem mass spectroscopy (UPLC-HDMS) in a "non-targeted" fashion and high-performance liquid chromatography (HPLC) in a "targeted" fashion. Four main compounds were identified, and a metabolic study was conducted on each to establish their possible significance for Pb(II) bioremoval. The study investigates the first metabolic profile to date for Pb(II) bioremoval, which in turn can result in a clarified understanding for development on an industrial and microbial level.


Assuntos
Biomassa , Chumbo/metabolismo , Metaboloma , Consórcios Microbianos , Águas Residuárias/microbiologia , Poluentes Químicos da Água/metabolismo , Biodegradação Ambiental
3.
Environ Sci Pollut Res Int ; 28(41): 57997-58006, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34100211

RESUMO

This study assessed prophylactic potentials of silymarin against lead-induced hepatorenal toxicity in rats with the respect to its antioxidant and anti-apoptotic activities. Forty male albino rats were distributed into four groups. Control group is provided with distilled water. Lead acetate group was given lead acetate (100 mg/kg bwt) orally for 10 weeks. The third and fourth groups administered silymarin at doses of 50 or 100 mg/kg bwt, respectively, 1 h before administration of lead acetate for 10 weeks. Lead acetate altered liver structure and function that represented by significant elevation of the activities of serum aspartate and alanine aminotransferases and serum levels of urea and creatinine. Hepatic and renal tissues' malondialdehyde concentrations were increased, while reduced glutathione content and superoxide dismutase and catalase activities were reduced in the lead acetate group. Also, lead acetate increased caspase-3 mRNA expression and inhibited alpha-fetoprotein mRNA expression in hepatic tissues, as well as it altered liver and kidney tissues' architectures. In contrast, silymarin ameliorated in a dose dependent mannar the toxic effects of lead acetate on the liver and kidneys through modulation of lead acetate which altered liver and kidney function and structures via reducing lipid oxidation and pathological changes of hepatic and renal tissue structure, improving antioxidant defense system of liver and kidneys, and decreasing pro-apoptotic gene expression in hepatic tissue. This study indicated that silymarin ameliorated lead acetate-induced hepatorenal toxicity via its antioxidant and cytoprotective potentials.


Assuntos
Antioxidantes , Silimarina , Animais , Antioxidantes/metabolismo , Glutationa/metabolismo , Rim/metabolismo , Chumbo/metabolismo , Peroxidação de Lipídeos , Fígado/metabolismo , Masculino , Estresse Oxidativo , Ratos , Silimarina/metabolismo , Silimarina/farmacologia
4.
Molecules ; 26(10)2021 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-34065439

RESUMO

There are many controversies regarding the relationship between lead exposure andcomplications in pregnancy. Preeclampsia (PE) is a maternal hypertensive disorder which is one of the main causes of maternal and foetal mortality. The aim of our study was to assess blood lead level (BLL) in Polish women with PE (PE group, n = 66) compared with healthy, non-pregnant women (CNP group, n = 40) and healthy pregnant women (CP group, n = 40). BLL was determined by inductively coupled plasma mass spectrometry (ICP-MS). The systolic blood pressure (SBP), diastolic blood pressure (DBP) and BLL in the CP group were significantly lower than in the PE group (p < 0.001). Logistic regression analyses of BLL showed a significant positive relationship with the presence of PE. Furthermore, both the SBP and DBP values were positively associated with BLL. This study indicates that preeclamptic women tend to present with significantly higher BLL compared to healthy pregnant women. There were no differences in the BLL between the CP and CNP groups.


Assuntos
Chumbo/metabolismo , Exposição Ocupacional , Pré-Eclâmpsia/metabolismo , Adolescente , Adulto , Pressão Sanguínea , Estudos de Casos e Controles , Feminino , Humanos , Espectrometria de Massas/métodos , Pessoa de Meia-Idade , Polônia , Gravidez , Adulto Jovem
5.
Ecotoxicol Environ Saf ; 220: 112410, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34126303

RESUMO

Lead (Pb) toxicity is a growing serious environmental pollution that threatens human health and crop productivity. Poplar, as an important economic and ecological forest species, has the characteristics of fasting growth and accumulating heavy metals, which is a powerful model plant for phytoremediation. Here, a novel label-free quantitative proteomic platform of SWATH-MS was applied to detect proteome changes in poplar seedling roots following Pb treatment. In total 4388 unique proteins were identified and quantified, among which 542 proteins showed significant abundance changes upon Pb(II) exposure. Functional categorizations revealed that differentially expressed proteins (DEPs) primarily distributed in specialized biological processes. Particularly, lignin and flavonoid biosynthesis pathway were strongly activated upon Pb exposure, implicating their potential roles for Pb detoxification in poplar. Furthermore, hemicellulose and pectin related cell wall proteins exhibited increased abundances, where may function as a sequestration reservoir to reduce Pb toxicity in cytoplasm. Simultaneously, up-regulation of glutathione metabolism may serve as a protective role for Pb-induced oxidative damages in poplar. Further correlation investigation revealed an extra layer of post-transcriptional regulation during Pb response in poplar. Overall, our work represents multiply potential regulators in mediating Pb tolerance in poplar, providing molecular targets and strategies for phytoremediation.


Assuntos
Chumbo/toxicidade , Metais Pesados/toxicidade , Populus/efeitos dos fármacos , Proteoma/efeitos dos fármacos , Biodegradação Ambiental , Vias Biossintéticas/efeitos dos fármacos , Chumbo/metabolismo , Metais Pesados/metabolismo , Proteínas de Plantas/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/metabolismo , Populus/metabolismo , Proteoma/metabolismo , Proteômica , Plântula/efeitos dos fármacos , Plântula/metabolismo
6.
Ecotoxicol Environ Saf ; 221: 112429, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34147864

RESUMO

Rare earth elements (REEs) are emerging as a serious threat to ecological safety due to their increasing accumulation in environments. The accumulation of REEs in environments has significantly increased its accumulation in the leaves of edible plants. However, the accumulation pathway of REEs in the leaves of edible plants are still unknown. In this study, lanthanum [La(III), a widely used and accumulated REE] and four edible plants (soybean, lettuce, pakchoi, and celery) with short growth cycles were selected as research objects. By using interdisciplinary research techniques, we found that low-dose La(III) activated endocytosis (mainly the clathrin-mediated endocytosis) in the leaf cells of four edible plants, which provided an accumulation pathway for low-dose La in the leaf cells of these edible plants. The accumulation of La in the leaf cells was positively correlated with the intensity of endocytosis, while the intensity of endocytosis was negatively correlated with the density of leaf trichomes. In addition to the accumulation of La, low-dose La(III) also brought other risks. For example, the harmful element (Pb) can also be accumulated in the leaf cells via La(III)-activated endocytosis; the homeostasis of the essential elements (K, Ca, Fe, Mg) was disrupted, although the chlorophyll synthesis and the growth of these leaf cells were accelerated; and the expression of stress response genes (GmNAC20, GmNAC11) in soybean leaves was increased. These results provided an insight to further analyze the toxicity and mechanism of REEs in plants, and sounded the alarm for the application of REEs in agriculture.


Assuntos
Endocitose/efeitos dos fármacos , Homeostase/efeitos dos fármacos , Lantânio/metabolismo , Lantânio/toxicidade , Chumbo/metabolismo , Folhas de Planta/efeitos dos fármacos , Plantas Comestíveis/efeitos dos fármacos , Agricultura , Metais Terras Raras/metabolismo , Metais Terras Raras/toxicidade , Folhas de Planta/metabolismo , Plantas Comestíveis/metabolismo , Oligoelementos/metabolismo
7.
Environ Sci Pollut Res Int ; 28(38): 53668-53678, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34036504

RESUMO

Lead (Pb) is one of the most common environmental pollutants and causes adverse effects on human and animal health. This study aimed to evaluate the protective role of beta-glucan against hepatic and reproductive toxicity induced by lead acetate. A total of 28 Sprague Dawley male rats were distributed into four groups (n = 7). The control group was intraperitoneally injected saline (1 ml/kg b.w.) daily for 21 days, the Pb group was intraperitoneally injected lead acetate (15 mg/kg b.w.) daily for 21 days, the beta-glucan group was orally administrated beta-glucan (50 mg/kg b.w.) daily for 21 days, and the Pb + beta-glucan group was intraperitoneally injected lead acetate (15 mg/kg b.w.) daily following the oral administration of beta-glucan (50 mg/kg b.w.) daily for 21 days. Results showed that feed intake in the Pb + beta-glucan group was significantly increased in comparison with that of the Pb group (p < 0.001). We also found that liver malondialdehyde (MDA) level was increased significantly in the Pb group (p < 0.01), while glutathione (GSH) level (p < 0.05), glutathione peroxidase (GSH-Px) (p < 0.05), and catalase (CAT) (p < 0.01) activities were reduced when they were compared with control. Moreover, Pb administration increased expression of pro-apoptotic protein Bax, the ratio of Bax/Bcl-2, and decreased the expression of the antiapoptotic protein Bcl-2 (p < 0.01). Also, Pb was found to cause a significant decrease in sperm motility (p < 0.01) and sperm concentration (p < 0.05) but increase in sperm tails and total sperm anomalies (p < 0.05). These findings were partially preserved by the administration of beta-glucan. Taken together, these results indicated that beta-glucan has the potential to alleviate the Pb-induced toxicity.


Assuntos
Motilidade Espermática , beta-Glucanas , Acetatos/metabolismo , Animais , Antioxidantes/metabolismo , Chumbo/metabolismo , Chumbo/toxicidade , Fígado , Masculino , Malondialdeído/metabolismo , Estresse Oxidativo , Ratos , Ratos Sprague-Dawley , Saccharomyces cerevisiae , Testículo/metabolismo , beta-Glucanas/metabolismo
8.
Gene ; 791: 145714, 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-33979680

RESUMO

Lead (Pb) is a serious heavy metal soil pollutant. It can be absorbed and accumulated by plant roots and impact plant growth. Medicago sativa L. (alfalfa) is a low-input forage and potential bioenergy crop, and improving its yield and quality has always been a focus of the alfalfa breeding industry. Little is known about the mechanism by which alfalfa responds to Pb stress at the molecular level. In this study, three alfalfa genotypes (a lead-resistant type (LR), a lead-sensitive type (LS) and an intermediate type (IN)) with contrasting abilities to resist lead were exposed to different durations of Pb treatment. Next-generation sequencing (NGS)-based RNA-seq technology was employed to characterize the root transcriptomes of three genotypes of alfalfa and identify differentially expressed genes (DEGs) during Pb stress. Genotypes LR and LS displayed different mechanisms of tolerance. In LR, the accumulation of more resistant substances was induced by the upregulation of sucrose synthase, glucan endo-1,3-beta-glucosidase, beta-amylase 3, probable trehalose-phosphate phosphatase J, 6-phosphofructo-2-kinase delta-1-pyrroline-5-carboxylate synthase (P5CS) and δ-ornithine aminotransferase (δ-OAT). In addition, flavin monooxygenase (YUCCA), 4-coumarate:CoA ligase-like protein (4CL), cinnamoyl-CoA reductase-like protein (CCR), ferulate 5-hydroxylase (F5H) and caffeic acid O-methyltransferase (COMT) were upregulated, leading to root development in a short time under Pb stress. Further study of the expression levels of metal transport-related genes, such as NRAMP (metal transporter), MATE (multidrug and toxin extrusion), HIPPs (heavy metal-associated isoprenylated plant proteins), MTP (metal tolerance protein), and ABC transporter, suggested that these genes were differentially expressed after lead treatment in the three alfalfa genotypes. Our research provides useful information for further studies on the molecular mechanism of Pb resistance in Medicago sativa L.


Assuntos
Chumbo/metabolismo , Medicago sativa/genética , Estresse Fisiológico/genética , Sequência de Bases/genética , Expressão Gênica/genética , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica de Plantas/genética , Genótipo , Chumbo/efeitos adversos , Medicago sativa/metabolismo , Fenótipo , Raízes de Plantas/genética , Transcriptoma/genética
9.
Int Microbiol ; 24(3): 441-453, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33987705

RESUMO

Globally, the underlying peril of cumulative toxicity of heavy metals in water bodies contaminated by industrial effluents is a matter of great concern to the environmentalists. Heavy metals like lead, cadmium, and nickel are particularly liable for this. Such toxic water is not only hazardous to human health but also harmful to aquatic animals. Remedial measures are being taken by physico-chemical techniques, but most of them are neither eco-friendly nor cost-effective. Biological means like bioaccumulation of heavy metals by viable bacteria are often tedious. In the present study, biosorption of heavy metals is successfully expedited by surfactant exopolysaccharide (SEPS) of Ochrobactrum pseudintermedium C1 as a simple, safe, and economically sustainable option utilizing an easily available and cost-effective substrate like molasses extract. Its efficacy in bioremediation of toxic heavy metals like cadmium, nickel, and lead have been studied by UV-Vis spectrophotometry and verified by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). FTIR and zeta potential studies have also been carried out to explore this novel biosorption potential. Results are conclusive and promising. Moreover, this particular SEPS alone can remediate all these three toxic heavy metals in water. For futuristic applications, it might be a prospective and cost-effective resource for bioremediation of toxic heavy metals in aqueous environment.


Assuntos
Metais Pesados/metabolismo , Ochrobactrum/metabolismo , Polissacarídeos Bacterianos/metabolismo , Tensoativos/metabolismo , Poluentes Químicos da Água/metabolismo , Biodegradação Ambiental , Cádmio/metabolismo , Análise Custo-Benefício , Chumbo/metabolismo , Microscopia Eletrônica de Varredura , Níquel/metabolismo , Polissacarídeos Bacterianos/ultraestrutura
10.
Biomolecules ; 11(3)2021 03 17.
Artigo em Inglês | MEDLINE | ID: mdl-33802758

RESUMO

Heavy metals (HMs) toxicity represents a global problem depending on the soil environment's geochemical forms. Biochar addition safely reduces HMs mobile forms, thus, reducing their toxicity to plants. While several studies have shown that biochar could significantly stabilize HMs in contaminated soils, the study of the relationship of soil properties to potential mechanisms still needs further clarification; hence the importance of assessing a naturally contaminated soil amended, in this case with Paulownia biochar (PB) and Bamboo biochar (BB) to fractionate Pb, Cd, Zn, and Cu using short sequential fractionation plans. The relationship of soil pH and organic matter and its effect on the redistribution of these metals were estimated. The results indicated that the acid-soluble metals decreased while the fraction bound to organic matter increased compared to untreated pots. The increase in the organic matter metal-bound was mostly at the expense of the decrease in the acid extractable and Fe/Mn bound ones. The highest application of PB increased the organically bound fraction of Pb, Cd, Zn, and Cu (62, 61, 34, and 61%, respectively), while the BB increased them (61, 49, 42, and 22%, respectively) over the control. Meanwhile, Fe/Mn oxides bound represents the large portion associated with zinc and copper. Concerning soil organic matter (SOM) and soil pH, as potential tools to reduce the risk of the target metals, a significant positive correlation was observed with acid-soluble extractable metal, while a negative correlation was obtained with organic matter-bound metal. The principal component analysis (PCA) shows that the total variance represents 89.7% for the TCPL-extractable and HMs forms and their relation to pH and SOM, which confirms the positive effect of the pH and SOM under PB and BB treatments on reducing the risk of the studied metals. The mobility and bioavailability of these metals and their geochemical forms widely varied according to pH, soil organic matter, biochar types, and application rates. As an environmentally friendly and economical material, biochar emphasizes its importance as a tool that makes the soil more suitable for safe cultivation in the short term and its long-term sustainability. This study proves that it reduces the mobility of HMs, their environmental risks and contributes to food safety. It also confirms that performing more controlled experiments, such as a pot, is a disciplined and effective way to assess the suitability of different types of biochar as soil modifications to restore HMs contaminated soil via controlling the mobilization of these minerals.


Assuntos
Carvão Vegetal/química , Fracionamento Químico/métodos , Metais Pesados/química , Solo/química , Cádmio/química , Cádmio/isolamento & purificação , Cádmio/metabolismo , Carvão Vegetal/isolamento & purificação , Cobre/química , Cobre/isolamento & purificação , Cobre/metabolismo , Poluição Ambiental , Compostos Férricos/química , Compostos Férricos/metabolismo , Concentração de Íons de Hidrogênio , Lamiales/química , Chumbo/química , Chumbo/isolamento & purificação , Chumbo/metabolismo , Compostos de Manganês/química , Compostos de Manganês/metabolismo , Metais Pesados/isolamento & purificação , Metais Pesados/metabolismo , Microscopia Eletrônica de Varredura , Compostos Orgânicos/química , Compostos Orgânicos/metabolismo , Óxidos/química , Óxidos/metabolismo , Análise de Componente Principal , Sasa/química , Espectrometria por Raios X , Zinco/química , Zinco/isolamento & purificação , Zinco/metabolismo
11.
Biomolecules ; 11(4)2021 03 28.
Artigo em Inglês | MEDLINE | ID: mdl-33800689

RESUMO

Bones are metabolically active organs. Their reconstruction is crucial for the proper functioning of the skeletal system during bone growth and remodeling, fracture healing, and maintaining calcium-phosphorus homeostasis. The bone metabolism and tissue properties are influenced by trace elements that may act either indirectly through the regulation of macromineral metabolism, or directly by affecting osteoblast and osteoclast proliferation or activity, or through becoming part of the bone mineral matrix. This study analyzes the skeletal impact of macroelements (calcium, magnesium, phosphorus), microelements (fluorine), and heavy metals (lead), and discusses the concentration of each of these elements in the various bone tissues.


Assuntos
Osso e Ossos/metabolismo , Cálcio/metabolismo , Chumbo/metabolismo , Magnésio/metabolismo , Animais , Densidade Óssea/efeitos dos fármacos , Osso e Ossos/química , Cálcio/análise , Cálcio/farmacologia , Flúor/análise , Flúor/metabolismo , Flúor/farmacologia , Humanos , Chumbo/análise , Chumbo/toxicidade , Magnésio/análise , Magnésio/farmacologia , Fósforo/análise , Fósforo/metabolismo , Fósforo/farmacologia
12.
ScientificWorldJournal ; 2021: 2974786, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33679258

RESUMO

A phytoremediation process for lead (Pb) under laboratory conditions on contaminated soil from the Puchuncaví commune, Valparaíso Province, Chile, was carried out. It analyzed the phytoremediation potential of Sarcocornia neei (Lag.) M.A. Alonso and M.B. Crespo. The plants were propagated beforehand and extracted from the El Yali wetland, a RAMSAR 878 site in Valparaíso. Soil fertility and heavy metal concentration analyses of composite samples were conducted, complying with established protocols and standard methodology for chemical and metal analyses. These analyses were conducted in the Soil Analysis Laboratory of the Pontificia Universidad Católica de Valparaíso. The aim was to analyse not only the tissue of plants from both areas but also the soil to identify the changes in different conditions in which the plants live. To determine the type of inferential analysis to be performed, a normality test was applied; however, it was deemed unsuitable, and therefore, the contrasts were developed using nonparametric tests, particularly Wilcoxon. R project software was used in the tests, especially the RCommander package, together with the Jamovi free-license statistical spreadsheet application. The analyses results of the soil samples indicated high concentrations of heavy metals, predominantly Pb at a concentration of 77.97 mg/kg, acidic soil indicated by pH between 5.77 and 6.38, low levels of electrical conductivity, and the presence of organic matter. A phytoremediation efficiency of 99% on soil samples was achieved. Preliminary results were compared against international regulations on the concentration of metals in soil. The histological sections showed that individual plants probably adapted to their environmental conditions.


Assuntos
Biodegradação Ambiental , Chenopodiaceae/metabolismo , Chumbo/metabolismo , Poluentes do Solo/toxicidade , Chile , Humanos , Chumbo/química , Chumbo/toxicidade , Metais Pesados/química , Metais Pesados/metabolismo , Metais Pesados/toxicidade , Poluentes do Solo/metabolismo
13.
Sci Rep ; 11(1): 6606, 2021 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-33758248

RESUMO

High lead (Pb) concentration in soils is becoming a severe threat to human health. It also deteriorates plants, growth, yield and quality of food. Although the use of plant growth-promoting rhizobacteria (PGPR), biochar and compost can be effective environment-friendly amendments for decreasing Pb stress in crop plants, the impacts of their simultaneous co-application has not been well documented. Thus current study was carried, was conducted to investigate the role of rhizobacteria and compost mixed biochar (CB) under Pb stress on selected soil properties and agronomic parameters in mint (Mentha piperita L.) plants. To this end, six treatments were studied: Alcaligenes faecalis, Bacillus amyloliquefaciens, CB, PGPR1 + CB, PGPR2 + CB and control. Results showed that the application A. faecalis + CB significantly decreased soil pH and EC over control. However, OM, nitrogen, phosphorus and potassium concentration were significantly improved in the soil where A. faecalis + CB was applied over control. The A. faecalis + CB treatment significantly improved mint plant root dry weight (58%), leaves dry weight (32%), chlorophyll (37%), and N (46%), P (39%) and K (63%) leave concentration, while also decreasing the leaves Pb uptake by 13.5% when compared to the unamended control. In conclusion, A. faecalis + CB has a greater potential to improve overall soil quality, fertility and mint plant productivity under high Pb soil concentration compared to the sole application of CB and A. faecalis.


Assuntos
Carvão Vegetal/metabolismo , Compostagem/métodos , Chumbo/toxicidade , Mentha/efeitos dos fármacos , Rizosfera , Poluentes do Solo/toxicidade , Alcaligenes faecalis/enzimologia , Alcaligenes faecalis/metabolismo , Aminoidrolases/metabolismo , Bacillus amyloliquefaciens/enzimologia , Bacillus amyloliquefaciens/metabolismo , Proteínas de Bactérias/metabolismo , Biodegradação Ambiental , Frutas/química , Chumbo/metabolismo , Mentha/microbiologia , Poluentes do Solo/metabolismo , Estresse Fisiológico , Verduras/química
14.
Angew Chem Int Ed Engl ; 60(22): 12381-12385, 2021 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-33759306

RESUMO

Lead (Pb) is a ubiquitous poisonous metal, affecting the health of vast populations worldwide. Medications to treat Pb poisoning suffer from various limitations and are often toxic owing to insufficient metal selectivity. Here, we report a cyclic tetrapeptide that selectively binds Pb and eradicates its toxic effect on the cellular level, with superior potency than state-of-the-art drugs. The Pb-peptide complex is remarkably strong and was characterized experimentally and computationally. Accompanied by the lack of toxicity and enhanced stability of this peptide, these qualities indicate its merit as a potential remedy for Pb poisoning.


Assuntos
Chumbo/química , Oligopeptídeos/química , Peptídeos Cíclicos/química , Sobrevivência Celular/efeitos dos fármacos , Células HT29 , Humanos , Chumbo/metabolismo , Chumbo/toxicidade , Oligopeptídeos/metabolismo , Peptídeos Cíclicos/metabolismo , Ligação Proteica
15.
Arch Microbiol ; 203(5): 2379-2392, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33665708

RESUMO

Identification and characterization of endogenous and stress adapted bacterial species, from rat-hole coal mines in Meghalaya, amplify the ambit of bioremediation for eco-restoration. 52 native bacterial isolates, drawn from soil and water samples of these mines, were analysed for bioremediation potential, based on growth and metal tolerance parameters. 12 of these isolates were metal tolerant with Bacillus spp. being the most promising taxon. Three isolates, namely, Serratia marcescens KH-CC, Bacillus altitudinis KH-16F and Bacillus siamensis KH-12A, exhibited high Maximum Tolerable Concentration (MTC) against Fe (500 ppm), Mn (830 ppm) and Pb (1400 ppm). B. siamensis showed highest Fe remediation with 48.34% removal capacity, while maximum removal for Mn and Pb was exhibited by Serratia marcescens at 72.5 and 83%, respectively. The growth profile of the isolates indicated their ability to survive under pH, temperature and salt stress conditions. In vitro growth kinetics studies of the isolates revealed their ability to decrease the acidity of growth media and improve alkalinity from an initial of pH 4.8-5.2 to an alkaline level of pH 8.5-9. These native bacteria, extracted from the stressed coal mine habitat, are potential germane applicants for rehabilitation and eco-restoration of ecologically degraded mine sites.


Assuntos
Bactérias/classificação , Fenômenos Fisiológicos Bacterianos , Biodegradação Ambiental , Minas de Carvão , Metais Pesados/metabolismo , Bactérias/efeitos dos fármacos , Bactérias/isolamento & purificação , Técnicas de Tipagem Bacteriana , Farmacorresistência Bacteriana , Ecossistema , Ferro/metabolismo , Chumbo/metabolismo , Manganês/metabolismo , Filogenia , RNA Ribossômico 16S/genética , Solo/química , Microbiologia do Solo , Poluentes do Solo/metabolismo , Microbiologia da Água
16.
Int J Mol Sci ; 22(4)2021 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-33669601

RESUMO

Lead (Pb) is one of the major heavy metals that are toxic to vertebrates and usually considered as environmental pollutants. ABCC4/MRP4 is an organic anion transporter that mediates cellular efflux of a wide range of exogenous and endogenous compounds such as cyclic nucleotides and anti-cancer drugs; however, it remains unclear whether ABCC4 and its orthologs function in the detoxification and excretion of toxic lead. In this study, we found that the transcriptional and translational expression of zebrafish abcc4 was significantly induced under lead exposure in developing zebrafish embryos and adult tissues. Overexpression of zebrafish Abcc4 markedly decreased the cytotoxicity and accumulation of lead in pig renal proximal tubule cell line (LLC-PK1 cells). To further understand the functions of zebrafish Abcc4 in lead detoxification, the clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system was used to create an abcc4-/- mutant zebrafish line. In comparison with the wild-type (WT) zebrafish, the abcc4-/- mutants showed a higher death rate and lead accumulation upon exposure to lead. Furthermore, a stable abcc4-transgenic zebrafish line was successfully generated, which exerted stronger ability to detoxify and excrete lead than WT zebrafish. These findings indicate that zebrafish Abcc4 plays a crucial role in lead detoxification and cellular efflux and could be used as a potential biomarker to monitor lead contamination in a water environment.


Assuntos
Técnicas de Inativação de Genes , Chumbo/metabolismo , Proteínas Associadas à Resistência a Múltiplos Medicamentos/metabolismo , Proteínas de Peixe-Zebra/metabolismo , Peixe-Zebra/genética , Animais , Animais Geneticamente Modificados , Sequência de Bases , Transporte Biológico , Regulação da Expressão Gênica no Desenvolvimento , Inativação Metabólica , Células LLC-PK1 , Proteínas Associadas à Resistência a Múltiplos Medicamentos/genética , Mutação/genética , Suínos , Peixe-Zebra/embriologia , Proteínas de Peixe-Zebra/genética
17.
Chemosphere ; 276: 130111, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33691221

RESUMO

BACKGROUND: Lead (Pb) is considered an endocrine-disrupting chemical. However, few studies have investigated the effects of low-level Pb exposure on plasma glucose levels. Herein, we aimed to investigate whether low-level Pb exposure causes elevated plasma glucose levels and the possible mechanisms involved. METHODS: We conducted a cross-sectional study of 5747 participants from 16 sites in China. The participants underwent measurements of anthropometric factors, blood lead level (BLL) and fasting plasma glucose (FPG). Wistar rats were exposed to 0.05% Pb through drinking water or fed with a high-fat diet (HFD) for 28 weeks. The relevant parameters of glucose homeostasis, hepatic glucose production (HGP) and gene expression levels of hepatic gluconeogenesis enzymes, including phosphoenolpyruvate carboxy kinase (PEPCK), glucose-6-phosphatase (G6PC) and fructose-1,6-bisphosphatase (FBP1), were measured. In addition, gene expression levels of gluconeogenesis enzymes were also measured in HepG2 cells administered with different concentrations of lead acetate for 24 h. RESULTS: In humans, after adjusting for confounders, the odds of having High_FPG (≥5.6 mmol/L) were significantly increased by 25% in the participants in the fourth BLL quartile (OR 1.25, 95% CI 1.05, 1.49). In the animals exposed to 0.05% Pb, FPG, HGP and hepatic gene expression levels of PEPCK, G6PC and FBP1 were increased. In addition, the mRNA expression levels of PEPCK, G6PC and FBP1 in HepG2 cells were also increased in response to Pb exposure. CONCLUSIONS: These findings support the possibility that low-level Pb exposure may increase HGP by affecting key enzymes of hepatic gluconeogenesis, eventually resulting in impaired FPG and hyperglycemia.


Assuntos
Gluconeogênese , Chumbo , Animais , Glicemia/metabolismo , China , Estudos Transversais , Jejum , Glucose/metabolismo , Chumbo/metabolismo , Chumbo/toxicidade , Fígado/metabolismo , Ratos , Ratos Wistar
18.
J Sci Food Agric ; 101(12): 5142-5153, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33608880

RESUMO

BACKGROUND: Lead (Pb2+ ) is one of the most toxic heavy metals and can be found in various quantities in the environment. The five native probiotic bacteria and inulin were used to assess in vitro lead nitrate and lead acetate binding capacities, as well as removal potentials. RESULTS: The highest decrease in media pH was seen for samples containing a combination of Lactobacillus paracasei IRBC-M 10784, lead nitrate and inulin (5.30 ± 0.012). The presence of inulin in the environment accelerated decreases in the pH of all samples with no significance. In all groups, lead nitrate-containing samples included maximum pH decreases. From the highest to the lowest, the ability of lead removal was linked to Lactobacillus acidophilus PTCC-1932 (88.48%), Bifidobacterium bifidum BIA-7 (85.32%), Bifidobacterium lactis BIA-6 (85.24%), Lactobacillus rhamnosus IBRC-M 10782 (83.18%) and L. paracasei IRBC-M 10784 (80.66%). Most species included the highest decrease in lead nitrate. Fourier-transform infrared spectroscopy (FTIR) analysis demonstrated that various functional groups (hydroxyl, carboxylic, carbonyl, amino and amide binds) on the bacterial cell wall were involved in lead ion binding during incubation. Principal component analysis of the FTIR results showed differences with respect to treated groups and control groups. CONCLUSION: The results obtained in the present study reveal that the simultaneous use of native probiotics and inulin can be an effective and safe approach for removing various toxic substances, especially Pb. © 2021 Society of Chemical Industry.


Assuntos
Bifidobacterium/metabolismo , Inulina/química , Lactobacillus/metabolismo , Chumbo/metabolismo , Nitratos/metabolismo , Compostos Organometálicos/metabolismo , Adsorção , Bifidobacterium/química , Biodegradação Ambiental , Parede Celular/química , Parede Celular/metabolismo , Concentração de Íons de Hidrogênio , Lactobacillus/química , Chumbo/química , Nitratos/química , Compostos Organometálicos/química , Probióticos/química , Probióticos/metabolismo
19.
Ecotoxicol Environ Saf ; 212: 112014, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33548569

RESUMO

The biosorption and bioaugmentation performances of Mucor circinelloides were investigated under different contact time, initial metal(loid) concentration and species. The microbe-plant interaction appeared synergistic with enhancing plant growth and alleviating oxidative damages induced by lead, cadmium and arsenic. The bioaugmentation with M. circinelloides led to significant immobilization on lead, cadmium and arsenic as indicated by the decreases of metal(loid) transfer and bioavailability in plant-microbe aqueous system. Lead, cadmium and arsenic were mainly allocated on cell wall and a few parts entered into intercellular system, suggesting cell wall adsorption and intracellular bioaccumulation served as the main mechanisms of M. circinelloides. The adsorption kinetics and isotherms on lead, cadmium and arsenic were fitted well with the pseudo-second-order and Langmuir models, with the maximum adsorption capacities of 500, 15.4 and 29.4 mg·g-1 fungal biomass at pH 6.0 and 25 â„ƒ. The optimum initial concentration and contact time were 300-10-20 mg·L-1 and 2 h. This study provides a basis for M. circinelloides as a promising adsorbent and bioaugmented agent for the cleanup of soil/aqueous environment contaminated with lead, cadmium and arsenic.


Assuntos
Arsênio/metabolismo , Cádmio/metabolismo , Chumbo/metabolismo , Mucor/metabolismo , Poluentes do Solo/metabolismo , Solanum nigrum , Adsorção , Arsênio/análise , Bioacumulação , Biodegradação Ambiental , Disponibilidade Biológica , Biomassa , Cádmio/análise , Concentração de Íons de Hidrogênio , Cinética , Chumbo/análise , Modelos Teóricos , Mucor/crescimento & desenvolvimento , Poluentes do Solo/análise , Solanum nigrum/metabolismo , Solanum nigrum/microbiologia
20.
Biol Trace Elem Res ; 199(12): 4700-4712, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33452669

RESUMO

Lead (Pb), a toxic pollutant, is toxic to the testis. However, biological events during testicular Pb poisoning were not well understood. Selenium (Se) has the ability to antagonize Pb toxicity. The purpose of this research was to clarify the relief mechanism of Se on testicular toxicity of Pb from the perspective of oxidative stress, inflammation, heat shock response, and autophagy in a chicken model. Sixty male Hyline chickens (7-day-old) were randomly assigned into four groups. The feeding program consisted of a commercial diet, a Se-supplemented diet (1 mg kg-1 Se), a Pb-supplemented diet (350 mg L-1 Pb), and a Se- and Pb-supplemented diet, respectively. On the 12th week, serums were collected to measure testosterone level and testes were removed to determine testis weight, histological structure, Pb and Se concentrations, oxidative stress indicators, and mRNA and protein expression of inflammatory cytokines, heat shock proteins, and autophagy-related genes. The results showed that Pb poisoning changed the histological structure of testes; decreased serum testosterone level, testis weight, catalase, glutathione-s-transferase, and total antioxidative capacity activities; increased hydrogen peroxide content; inhibited interleukin (IL)-2 and mammalian target of rapamycin expression; and promoted IL-4, IL-12ß, heat shock proteins, Beclin 1, Dynein, autophagy-related proteins 5, light chain 3 (LC3)-I, and LC3-II expression in the testes of chickens. Se intervention mitigated the aforementioned alterations induced by Pb. In conclusion, Pb led to oxidative stress, which triggered inflammation, heat shock response, and autophagy. Se administration mitigated testicular toxicity of Pb mainly by mitigating oxidative stress in male chickens.


Assuntos
Selênio , Animais , Autofagia , Galinhas , Resposta ao Choque Térmico , Inflamação/induzido quimicamente , Inflamação/metabolismo , Chumbo/metabolismo , Chumbo/toxicidade , Masculino , Estresse Oxidativo , Selênio/metabolismo , Selênio/farmacologia , Testículo/metabolismo
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