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1.
Estima (Online) ; 19(1): e0121, jan.-dez. 2021.
Artigo em Português | BDENF - Enfermagem | ID: biblio-1151133

RESUMO

Objetivo:avaliar a qualidade de vida de pessoas com úlcera venosa e correlacionar com as características clínicas e sintomas associados à ferida. Método: estudo quantitativo, transversal e analítico. Para as análises das associações das variáveis qualitativas foram aplicados os testes Qui-quadrado e exato de Fisher, e para as variáveis quantitativas, o coeficiente de correlação de Spearman. Utilizou-se o instrumento Pressure Ulcer Scale for Healing para a avaliação da ferida e o Freiburg Life Quality Assessment Wound-wk para análise da qualidade de vida. Fizeram parte do estudo 103 sujeitos com tempo de feridas superior a seis semanas, de etiologia venosa. Resultados: houve correlação das características clínicas da úlcera venosa e sintomas associados com a qualidade de vida. Nas dimensões sintomas físicos, pessoas com úlcera venosa apresentraram pior escore em relação ao sintoma dor (p<0,0001). Associadas a isso, as características clínicas como a aparência (p< 0,0001) e a área (p=0,0037) da ferida também manifestaram correlação. Em relação à escala de avaliação da ferida (área, tecido na ferida e exsudato), o pior escore interferiu nos sintomas físicos. Conclusão: a dor, tamanho da ferida e seu aspecto influenciam negativamente na qualidade de vida de pessoas com úlcera venosa


Assuntos
Qualidade de Vida , Úlcera Varicosa , Cicatrização , Enfermagem
2.
Estima (Online) ; 19(1): e0621, jan.-dez. 2021.
Artigo em Português | BDENF - Enfermagem | ID: biblio-1255201

RESUMO

Objetivo:avaliar taxa de cicatrização em úlceras do pé diabético tratadas com biomembrana de proteínas do látex de Calotropis procera (BioMem CpLP) quando comparada ao hidrocoloide em pó. Método: ensaio clínico randomizado controlado, registrado pelo Registro Brasileiro de Ensaio Clínico (REBEC), conforme protocolo RBR-98f3j9, realizado junto a oito pessoas com pé diabético, em ambulatório de pé diabético, de março a julho de 2019. No grupo experimental (n=04), realizou-se a aplicação de biomembrana; no grupo controle (n=04), utilizou-se o pó de hidrocoloide. A taxa de cicatrização foi avaliada com 30 e 60 dias após início do tratamento. Resultados: não se constataram diferenças estatísticas entre taxas de cicatrização do grupo controle e do grupo experimental na análise temporal dos 30 dias iniciais (p=0,726) e nos 60 dias subsequentes ao início do tratamento (p=0,562). Conclusão: a BioMem CpLP apresentou taxas de cicatrização semelhantes ao produto convencional, configurando-se como alternativa eficaz e de baixo custo para o tratamento de pés diabéticos.


Objective:to evaluate the rate of healing in diabetic foot ulcers treated with a biomembrane of latex proteins from Calotropis procera (BioMem CpLP) when compared to powdered hydrocolloid. Method: randomized controlled clinical trial, registered by the Brazilian Clinical Trial Registry (REBEC), according to protocol RBR-98f3j9, carried out with eight people with diabetic foot, in a diabetic foot clinic, from March to July 2019. In the experimental group (n = 04), biomembrane was applied; in the control group (n = 04), hydrocolloid powder was used. The healing rate was assessed at 30 and 60 days after starting treatment. Results: no statistical differences were found between the healing rates of the control group and the experimental group in the temporal analysis of the initial 30 days (p = 0.726) and in the 60 days following the start of treatment (p = 0.562). Conclusion: BioMem CpLP presented healing rates similar to the conventional product, being an effective and low cost alternative for the treatment of diabetic feet.


Assuntos
Cicatrização , Enfermagem , Ensaio Clínico Controlado Aleatório , Pé Diabético
3.
Pol Merkur Lekarski ; 49(291): 209-212, 2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34218240

RESUMO

Oncological diseases remain the most important problem of medicine. Causes of cancer, essence of cells malignant transformation process is not revealed, mechanisms of occurrence and development of cancer diseases are not determined. Recent studies show neutrophils are found in close proximity to tumor cells. The provision that neutrophils can be really important cells in human cancer development is detail discussed. AIM: The aim of the study was based on literature data and the results of our own studies develop a concept to explain and evaluate the role of neutrophils in oncogenesis. MATERIALS AND METHODS: Analysis of modern foreign and domestic literature, the results of our earlier numerous experiments in vivo and in vitro was conducted. Experiments were performed on 12 outbreed male rats weighing 190-215 g in compliance with the EU Directive 2010/63/ EU for animal experiments. The wounds were made under ether anesthesia by excision in the interscapular region of the skin with subcutaneous fiber an area of 300 mm2. The reaction of cells was studied in dynamics from the moment of wound application to their healing by smears-prints taken from the wound surface according to the method proposed by M.P. Pokrovskaya and M.S. Makarov. In order to obtain more reliable information about the dynamics of cell change in the wound, cytological studies of the same wound were carried out repeatedly during the day. RESULTS: Large number of cells was found on the wound surface, the bulk of which were neutrophils. After 1-2 hours, no morphologically normal cells could be detected in the preparations. Against the background of the small pink-violet granularity, which covered the entire specimen, it was possible to see only single nuclei with indistinct contours for several fields of view. By 4 hours, the granularity formed as a result of the leukocytes disintegration increased in size, rounded, turning into bodies of spherical or oval shape of different diameters: from 1 to 5-7 µm, stained according to Romanovsky in the color of nuclear material. After 6-8 hours, the growth of spherical bodies continued. Their number also increased. Were noted many clusters of nuclear matter in the form of irregular shapes of large sizes. Smaller graininess was also retained. With an increase in the volume of spherical bodies, the following changes are possible. In the process of neutrophil cultivation, we were able to obtain data shedding additional light on the process of neutrophils transformation into poorly differentiated cells. It was shown that during the three-day cultivation, the total number of cells detected in the preparations increases, although to a much lesser extent than could be expected, based on the number of separated segments of neutrophil nuclei. CONCLUSIONS: It is highly probable that the cause of the cancer cells appearance in the human body lies in the reaction of neutrophils involved in inflammatory regeneration in response to the damaging effect of oncogenes, which may be various physical, chemical, biological factors, namely the ability of neutrophils to dedifferentiate.


Assuntos
Neoplasias , Neutrófilos , Animais , Masculino , Ratos , Pele , Cicatrização
4.
Mater Sci Eng C Mater Biol Appl ; 127: 112202, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34225854

RESUMO

Acellular Dermal Matrix (ADM) is mainly made with human or porcine skins and has the risk of zoonotic virus transmission. The fish skin-derived ADM could overcome the shortcoming. Fish skin acellular matrix has been used as wound dressing, but there is few systematic studies on tilapia-skin acellular dermal matrix (TS-ADM). In the present study, a novel TS-ADM was made by an alkaline decellularization process and γ-irradiation. The physical properties, biocompatibility, pre-clinical safety and wound healing activity of TS-ADM were systematically evaluated for its value as a functionally bioactive wound dressing. Histopathological analysis (hematoxylin and eosin staining, 4,6-diamidino-2-phenylindole (DAPI) staining) and DNA quantification both proved that the nuclear components of tilapia skin were removed sufficiently in TS-ADM. Compared to the commercial porcine acellular dermal matrix (DC-ADM), TS-ADM has distinctive features in morphology, thermal stability, degradability and water vapor transmission. TS-ADM was more readily degradable than DC-ADM in vitro and in vivo. In both rat and mini-pig skin wound healing experiments, TS-ADM was shown to significantly promote granulation growth, collagen deposition, angiogenesis and re-epithelialization, which may be attributed to the high expression of transforming growth factor-beta 1 (TGF-ß1), alpha-smooth muscle actin (α-SMA) and CD31. Herein, the novel TS-ADM, used as a low-cost bioactive dressing, could form a microenvironment conducive to wound healing.


Assuntos
Derme Acelular , Pele Artificial , Tilápia , Animais , Ratos , Transplante de Pele , Suínos , Porco Miniatura , Cicatrização
5.
Mater Sci Eng C Mater Biol Appl ; 127: 112204, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34225856

RESUMO

Alginate fibrous materials have been applied as wound dressing to enhance wound healing due to its nontoxic, biodegradable, and hemostatic nature. Conventional nonwoven fabrication tactics, however, showed weakness in inflammation, degradation stability and mechanical properties. Herein, the wet-spun alginate fibers were prepared by a novel wheel spinning technique, then knitted into wound dressing. Benefiting from optimized wet spinning parameters and the agglomeration of alginate multimers, the fibers were endowed with elevated mechanical performances and biodegradability, which allowed for the feasibility of knitting wound-care materials. Using the new wheel spinning technique, high strength alginate fibers with 173 MPa were produced with breaking strain up to 18% and toughness of 16.16 MJ*m-3. Meanwhile, alginate fibers with high breaking strain reaching 35% were produced with tensile strength of 135 MPa and toughness of 37.47 MJ*m-3. The overall mechanical performances of these alginate fibers with high breaking strain are significantly higher (up to 2 times) than those published in the literature in term of toughness. In vitro degradation evaluation revealed that this wet spun fibrous dressing had good aqueous absorbency (50%) and sustained biodegradation properties. Furthermore, the consequent cell viability study also proved that this alginate knitted fabric is biocompatible for being applied as wound dressing.


Assuntos
Alginatos , Materiais Biocompatíveis , Bandagens , Materiais Biocompatíveis/farmacologia , Hidrogéis , Cicatrização
6.
Mater Sci Eng C Mater Biol Appl ; 127: 112245, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34225884

RESUMO

Wound healing is a complicated process constituted of four successive physiological stages involving wound bleeding, inflammatory response, cell proliferation and tissue remodeling. During this period, bacteria can easily infect the wound. Therefore, we prepared a novel curcumin-loaded sandwich-like nanofibrous membrane (CSNM) using sequential electrospinning for the hemostasis, antibacterial and accelerate wound healing. The morphology of the nanofibrous membrane was analyzed by SEM. In addition, the water absorption capacity, water vapor transmission rate, water contact-angle, and in vitro drug release were all tested. Then in vitro and in vivo hemostatic experiments demonstrated that CSNM has a good hemostatic effect. Antioxidant effect was assessed by the DPPH radical scavenging method and CSNM presented a high antioxidant activity. Additionally, CSNM demonstrated excellent antibacterial activity by the disk diffusion method. Furthermore, the rat dorsal skin defects model revealed that the CSNM distinctly induced the granulation tissue grew, collagen deposition and epithelial tissue remodeling. Meanwhile, the results of the immunohistochemical staining showed that the CSNM can facilitate the expression of CD31 and TGF-ß in the early stage of the wound, thereby accelerating wound healing. In general, this study proved that the multifunctional CSNM has great potential as wound dressing in wound healing.


Assuntos
Curcumina , Nanofibras , Animais , Antibacterianos/farmacologia , Bandagens , Curcumina/farmacologia , Ratos , Tecnologia , Cicatrização
7.
J Wound Care ; 30(Sup7): S47-S53, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34256586

RESUMO

The treatment of diabetic foot ulcers is complex and costly with an increased risk for infection, which may even lead to amputation. This prospective case series aims to assess the effectiveness of a dehydrated amniotic membrane allograft combined with a bilayer dermal matrix for healing complicated foot ulcers in patients with comorbidities. A total of six patients with complicated full-thickness ulcers and comorbidities, such as diabetes and peripheral vascular disease were treated with this technique. Each wound was measured intraoperatively just before graft application, at 14 days after application, and then at weeks 4, 8, and 12. Changes in wound volume and area were compared over time. One patient had complete wound closure by week eight, a second patient by week 12. The other four patients had wounds that decreased in size during the course of 12 weeks. The mean decrease in wound volume was 73.5% post-removal of the bilayer dermal matrix after two weeks of application. At week 12, the mean decrease in wound area and volume were 93.2% and 97.1%, respectively. This case series provides initial evidence that the combination of dehydrated amniotic membrane allograft with bilayer dermal matrix promotes complete wound closure in patients with comorbidities that may impede wound healing. Further clinical trials are needed to confirm these results.


Assuntos
Diabetes Mellitus , Pé Diabético , Aloenxertos , Âmnio , Animais , Bovinos , Colágeno , Pé Diabético/cirurgia , Glicosaminoglicanos , Humanos , Estudos Prospectivos , Tendões , Cicatrização
8.
J Wound Care ; 30(Sup7): S28-S34, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34256587

RESUMO

BACKGROUND: Pilonidal sinus disease (PSD) is a chronic inflammatory disease affecting the soft tissue of the sacrococcygeal region and remains a challenging disease for clinicians to treat. The optimal treatment for PSD remains controversial and recent reports describe several different surgical approaches offering different benefits. Approximately 40% of initial incision and drainage cases require subsequent surgery. Due to high recurrence rates and postoperative complications, a more complex revision surgery involving a flap reconstruction may be required. We hypothesised that the combination of an extracellular matrix (ECM) graft with tissue flap reconstruction may decrease the postoperative complications and recurrence rates for PSD. METHOD: We report a retrospective case series using a surgical flap reconstruction with concomitant implantation of an ovine forestomach ECM graft under a fasciocutaneous flap with an off-midline closure for recurrent PSD, where previously surgical intervention had failed due to wound dehiscence and/or recurrent disease. RESULTS: The case series included six patients. After three weeks, all patients except one were fully healed, and the sixth was fully healed by week 4; all wounds remained fully healed at 12 weeks. All patients achieved good cosmesis and were able to return to normal function without any residual symptoms. CONCLUSION: This pilot case series explored augmenting a flap reconstruction for complex PSD with advanced ECM graft materials, demonstrating that it may improve outcomes and minimise typical complications seen in flap closure, such as inflammation, infection, haematoma/seroma and hypoperfusion. Although the study had a limited number of participants, long-term outcomes were promising and suggest that further studies are warranted.


Assuntos
Seio Pilonidal , Animais , Matriz Extracelular , Humanos , Recidiva Local de Neoplasia , Seio Pilonidal/cirurgia , Recidiva , Estudos Retrospectivos , Ovinos , Resultado do Tratamento , Cicatrização
9.
J Wound Care ; 30(7): 568-580, 2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34256596

RESUMO

OBJECTIVE: Despite treatment advances over the past 30 years, the societal impact of hard-to-heal wounds is increasingly burdensome. An unresolved issue is wound pain, which can make many treatments, such as compression in venous leg ulcers, intolerable. The aim of this review is to present the evidence and stimulate thinking on the use of electrical stimulation devices as a treatment technology with the potential to reduce pain, improve adherence and thus hard-to-heal wound outcomes. METHOD: A literature search was conducted for clinical studies up to August 2020 reporting the effects of electrical stimulation devices on wound pain. Devices evoking neuromuscular contraction or direct spinal cord stimulation were excluded. RESULTS: A total of seven publications (three non-comparative and four randomised trials) were identified with four studies reporting a rapid (within 14 days) reduction in hard-to-heal wound pain. Electrical stimulation is more widely known for accelerated healing and is one of the most evidence-based technologies in wound management, supported by numerous in vitro molecular studies, five meta-analyses, six systematic reviews and 30 randomised controlled trials (RCTs). Despite this wealth of supportive evidence, electrical stimulation has not yet been adopted into everyday practice. Some features of electrical stimulation devices may have hampered adoption in the past. CONCLUSION: As new, pocket-sized, portable devices allowing convenient patient treatment and better patient adherence become more widely available and studied in larger RCTs, the evidence to date suggests that electrical stimulation should be considered part of the treatment options to address the challenges of managing and treating painful hard-to-heal wounds.


Assuntos
Úlcera Varicosa , Estimulação Elétrica , Humanos , Dor , Cooperação do Paciente , Cicatrização
10.
J Wound Care ; 30(7): 534-542, 2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34256595

RESUMO

Objective: The prevalence and economic burden of wounds are growing. Any wound has the potential to become hard-to-heal and require frequent care. Clinicians need to find ways to absorb demand on services without compromising outcomes. Drivers of wound care efficiency-time-to-heal, frequency of dressing change and the incidence of complications-can be evaluated to shape future wound management. A survey of wound care was conducted by clinicians from five centres in Sweden over a one-week period, during which clinicians documented every wound once. At the time of surveying, 49% of wounds were considered to be improving, infection incidence was 11.7% and dressings were changed a mean of 2.2 times per week, with highly exuding wounds changed 6.9 times per week. The data highlighted the importance of diagnosing patient and wound characteristics in selecting treatments and organising care. Recognised gaps in diagnoses potentially identify opportunities to influence healing, complication incidence and intensity of nursing, thus reducing demand on resources. In conclusion, this survey highlights opportunities to reduce the burdens these drivers present. Through improved diagnosis and alignment to recognised care pathways, there is potential to improve patient outcomes and alleviate the strains placed upon wound care providers.


Assuntos
Bandagens , Cicatrização , Humanos , Infecção da Ferida Cirúrgica , Suécia/epidemiologia
11.
J Wound Care ; 30(7): 553-561, 2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34256600

RESUMO

BACKGROUND: Venous leg ulcers (VLUs) are hard-to-heal, recurrent and challenging to treat. Advanced wound care matrices (AWCMs) have been developed to supplement conventional therapies. These costly AWCMs warrant careful comparison as healthcare expenditures are subjected to increasing scrutiny. AIM: This study was designed to compare AWCMs in their ability to heal VLUs and their cost efficacy through a systematic review of randomised controlled trials (RCTs). METHOD: An organised search of Medline, Cochrane Library, Central and CINAHL databases identified RCTs that compared AWCMs to standard compression therapy in the healing of VLUs. Bias was assessed using the Effective Public Health Practice Project (EPHPP) Quality Assessment Tool for Quantitative Studies. Eight studies analysing bilayered skin substitute (BSS) (Apligraf), dehydrated human amnion/chorion membrane (dHACM) (Epifix), human fibroblast-derived dermal substitute (HFDDS) (Dermagraft), extracellular wound matrix (ECM) (Oasis), advanced matrix (AM) (Talymed) and matrix wound dressing (MWD) (Promogran) met the inclusion criteria. RESULTS: Four studies reported significant improvement over standard therapy: BSS, dHACM, ECM and AM. Incremental cost per additional successful treatment was determined for each trial, ranging from $2593 (MWD) to $210,800 (HFDDS). CONCLUSION: Our consolidated analysis of eight major RCTs of AWCMs in the treatment of VLUs revealed a great variation in clinical and cost efficacy among these products. The included trials were inconsistent in methodology, and these limitations should be noted, but, in the absence of RCTs to compare these products, our systematic review may serve as a guide for practitioners who seek to optimise wound healing while considering cost efficacy.


Assuntos
Úlcera Varicosa , Bandagens , Análise Custo-Benefício , Humanos , Úlcera Varicosa/terapia , Cicatrização
12.
Molecules ; 26(13)2021 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-34202733

RESUMO

Thymoquinone is a natural bioactive with significant therapeutic activity against multiple ailments including wound healing. The poor aqueous solubility and low skin permeability limit its therapeutic efficacy. The present investigation aimed to improve the biopharmaceutical attributes of thymoquinone to enhance its topical efficacy in wound healing. A nanoemulsion-based hydrogel system was designed and characterized as a nanotechnology-mediated drug delivery approach to improve the therapeutic efficacy of thymoquinone, utilizing a high-energy emulsification technique. The black seed oil, as a natural home of thymoquinone, was utilized to improve the drug loading capacity of the developed nanoemulsion system and reduced the oil droplet size to <100 nm through ultrasonication. The influence of formulation composition, and the ultrasonication process conditions, were investigated on the mean globule size and polydispersity index of the generated nanoemulsion. Irrespective of surfactant/co-surfactant ratio and % concentration of surfactant/co-surfactant mixture, the ultrasonication time had a significant (p < 0.05) influence on the mean droplet size and polydispersity index of the generated nanoemulsion. The developed nanoemulgel system of thymoquinone demonstrated the pseudoplastic behavior with thixotropic properties, and this behavior is desirable for topical application. The nanoemulgel system of thymoquinone exhibited significant enhancement (p < 0.05) in skin penetrability and deposition characteristics after topical administration compared to the conventional hydrogel system. The developed nanoemulgel system of thymoquinone exhibited quicker and early healing in wounded Wistar rats compared to the conventional hydrogel of thymoquinone, while showing comparable healing efficacy with respect to marketed silver sulfadiazine (1%) cream. Furthermore, histopathology analysis of animals treated with a developed formulation system demonstrated the formation of the thick epidermal layer, papillary dermis along with the presence of extensive and organized collagen fibers in newly healed tissues. The outcome of this investigation signifies that topical delivery of thymoquinone through nanoemulgel system is a promising candidate which accelerates the process of wound healing in preclinical study.


Assuntos
Benzoquinonas , Sistemas de Liberação de Medicamentos , Nanopartículas , Absorção Cutânea/efeitos dos fármacos , Pele/metabolismo , Cicatrização/efeitos dos fármacos , Administração Tópica , Animais , Benzoquinonas/química , Benzoquinonas/farmacocinética , Benzoquinonas/farmacologia , Emulsões , Nanopartículas/química , Nanopartículas/uso terapêutico , Ratos , Ratos Wistar , Pele/patologia
13.
Int J Mol Sci ; 22(12)2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-34203608

RESUMO

Poly(2-hydroxyethyl methacrylate) (pHEMA) as a biomaterial with excellent biocompatibility and cytocompatibility elicits a minimal immunological response from host tissue making it desirable for different biomedical applications. This article seeks to provide an in-depth overview of the properties and biomedical applications of pHEMA for bone tissue regeneration, wound healing, cancer therapy (stimuli and non-stimuli responsive systems), and ophthalmic applications (contact lenses and ocular drug delivery). As this polymer has been widely applied in ophthalmic applications, a specific consideration has been devoted to this field. Pure pHEMA does not possess antimicrobial properties and the site where the biomedical device is employed may be susceptible to microbial infections. Therefore, antimicrobial strategies such as the use of silver nanoparticles, antibiotics, and antimicrobial agents can be utilized to protect against infections. Therefore, the antimicrobial strategies besides the drug delivery applications of pHEMA were covered. With continuous research and advancement in science and technology, the outlook of pHEMA is promising as it will most certainly be utilized in more biomedical applications in the near future. The aim of this review was to bring together state-of-the-art research on pHEMA and their applications.


Assuntos
Tecnologia Biomédica , Poli-Hidroxietil Metacrilato/química , Anti-Infecciosos/farmacologia , Regeneração Óssea , Sistemas de Liberação de Medicamentos , Humanos , Cicatrização
14.
Int J Mol Sci ; 22(12)2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-34203627

RESUMO

Recently, many studies investigated the role of a specific type of stem cell named the endothelial progenitor cell (EPC) in tissue regeneration and repair. EPCs represent a heterogeneous population of mononuclear cells resident in the adult bone marrow. EPCs can migrate and differentiate in injured sites or act in a paracrine way. Among the EPCs' secretome, extracellular vesicles (EVs) gained relevance due to their possible use for cell-free biological therapy. They are more biocompatible, less immunogenic, and present a lower oncological risk compared to cell-based options. EVs can efficiently pass the pulmonary filter and deliver to target tissues different molecules, such as micro-RNA, growth factors, cytokines, chemokines, and non-coding RNAs. Their effects are often analogous to their cellular counterparts, and EPC-derived EVs have been tested in vitro and on animal models to treat several medical conditions, including ischemic stroke, myocardial infarction, diabetes, and acute kidney injury. EPC-derived EVs have also been studied for bone, brain, and lung regeneration and as carriers for drug delivery. This review will discuss the pre-clinical evidence regarding EPC-derived EVs in the different disease models and regenerative settings. Moreover, we will discuss the translation of their use into clinical practice and the possible limitations of this process.


Assuntos
Células Progenitoras Endoteliais/metabolismo , Vesículas Extracelulares/metabolismo , Especificidade de Órgãos , Regeneração/fisiologia , Animais , Humanos , Cicatrização
15.
Georgian Med News ; (314): 64-70, 2021 May.
Artigo em Russo | MEDLINE | ID: mdl-34248029

RESUMO

The aim of our study was to investigate the effectiveness of osteoplastic material based on MMSC - AT for the restoration of bone defects. To study the processes of bone remodeling, in the replacement of bone defects after tooth extraction, patients were divided into three groups. Group A - 26 patients where used "Kolapan-L" to fill the bone defect; group B - 28 patients where for augmentation used osteoplastic composition "Kolopan-L" + MMSC-AT + PRP; group C - 25 patients in whom the healing of the bone defect occurred under a blood clot. In the patients group B, after 1 year of observation, the activity of acid phosphatase was 13,35% p> 0.05 and 33,40%, p>0,05, p1<0.05 below the increase in alkaline phosphatase by 8,84, p> 0,05, and 15,35%, p>0,05 relative to similar data in groups A and C. The index of bone mineralization in patients of group B was 8,84% and 19,74% higher than in the operated groups A and C, respectively p, p1>0,05, p2<0,05. When conducting histological examination of bone biopsies after 6 months of observation, in patients of group B, the obtained trepan biopsies had the structure of bone tissue: bone marrow spaces and sometimes small particles of osteoplastic material were determined between trabeculae. Therefore, based on the results of the study, it is safe to say that our proposed osteoplastic composition based on MMSC - AT is a good alternative to traditional osteoplastic materials for use in modern dentistry.


Assuntos
Células-Tronco Mesenquimais , Tecido Adiposo , Remodelação Óssea , Osso e Ossos , Humanos , Cicatrização
16.
Int J Mol Sci ; 22(11)2021 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-34199463

RESUMO

Little is known about the ability for epithelial regeneration and wound healing in patients with inflammatory bowel diseases. We evaluated the epithelial proliferation and wound healing ability of patients with Crohn's disease (CD) using patient-derived intestinal organoids. Human intestinal organoids were constructed in a three-dimensional intestinal crypt culture of enteroscopic biopsy samples from controls and CD patients. The organoid-forming efficiency of ileal crypts derived from CD patients was reduced compared with those from control subjects (p < 0.001). Long-term cultured organoids (≥6 passages) derived from controls and CD patients showed an indistinguishable microscopic appearance and culturing behavior. Under TNFα-enriched conditions (30 ng/mL), the organoid reconstitution rate and cell viability of CD patient-derived organoids were significantly lower than those of the control organoids (p < 0.05 for each). The number of EdU+ cells was significantly lower in TNFα-treated organoids derived from CD patients than in TNFα-treated control organoids (p < 0.05). In a wound healing assay, the unhealed area in TNFα-treated CD patient-derived organoids was significantly larger than that of TNFα-treated control organoids (p < 0.001). The wound healing ability of CD patient-derived organoids is reduced in TNFα-enriched conditions, due to reduced cell proliferation. Epithelial regeneration ability may be impaired in patients with CD.


Assuntos
Proliferação de Células/genética , Doença de Crohn/terapia , Células Epiteliais/metabolismo , Organoides/crescimento & desenvolvimento , Adulto , Doença de Crohn/metabolismo , Doença de Crohn/patologia , Células Epiteliais/patologia , Feminino , Humanos , Íleo/crescimento & desenvolvimento , Íleo/lesões , Íleo/patologia , Mucosa Intestinal/crescimento & desenvolvimento , Mucosa Intestinal/patologia , Intestinos/diagnóstico por imagem , Intestinos/lesões , Masculino , Pessoa de Meia-Idade , Organoides/metabolismo , Regeneração/genética , Transdução de Sinais/genética , Células-Tronco/citologia , Células-Tronco/metabolismo , Fator de Necrose Tumoral alfa/genética , Cicatrização/genética
17.
Int J Mol Sci ; 22(11)2021 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-34198821

RESUMO

Photo-polymerized hydrogels are ideally suited for stem-cell based tissue regeneration and three dimensional (3D) bioprinting because they can be highly biocompatible, injectable, easy to use, and their mechanical and physical properties can be controlled. However, photo-polymerization involves the use of potentially toxic photo-initiators, exposure to ultraviolet light radiation, formation of free radicals that trigger the cross-linking reaction, and other events whose effects on cells are not yet fully understood. The purpose of this study was to examine the effects of hydrogen sulfide (H2S) in mitigating cellular toxicity of photo-polymerization caused to resident cells during the process of hydrogel formation. H2S, which is the latest discovered member of the gasotransmitter family of gaseous signalling molecules, has a number of established beneficial properties, including cell protection from oxidative damage both directly (by acting as a scavenger molecule) and indirectly (by inducing the expression of anti-oxidant proteins in the cell). Cells were exposed to slow release H2S treatment using pre-conditioning with glutathione-conjugated-garlic extract in order to mitigate toxicity during the photo-polymerization process of hydrogel formation. The protective effects of the H2S treatment were evaluated in both an enzymatic model and a 3D cell culture system using cell viability as a quantitative indicator. The protective effect of H2S treatment of cells is a promising approach to enhance cell survival in tissue engineering applications requiring photo-polymerized hydrogel scaffolds.


Assuntos
Técnicas de Cultura de Células/métodos , Hidrogéis/farmacologia , Sulfeto de Hidrogênio/farmacologia , Engenharia Tecidual , Sobrevivência Celular/efeitos dos fármacos , Humanos , Luz , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/efeitos da radiação , Polimerização/efeitos dos fármacos , Polimerização/efeitos da radiação , Impressão Tridimensional , Tecidos Suporte , Cicatrização/efeitos dos fármacos , Cicatrização/efeitos da radiação
19.
Rev Bras Enferm ; 74(3): e20201117, 2021.
Artigo em Inglês, Português | MEDLINE | ID: mdl-34287493

RESUMO

OBJECTIVES: to evaluate the effects of venous ulcer healing in patients after six months of conventional treatment and adjuvant low-power laser therapy. METHODS: prospective cohort study nested in a randomized clinical trial with 38 patients, allocated into an intervention group (conventional treatment and adjuvant laser therapy) and a control group (conventional treatment). Patients were followed up as outpatients, sociodemographic and clinical variables were collected, and indicators of the outcomes Wound healing: secondary intention (1103) and Tissue integrity: skin and mucous membranes(1101) of the Nursing Outcomes Classification. Generalized estimating equations, Kaplan-Meier tests, and robust Poisson regression were used in the analysis. RESULTS: the clinical indicators Decreased wound size and Scar formation showed a statistically significant difference in the intervention group, higher number of healed wounds, lower rate, longer time to relapse. CONCLUSIONS: laser therapy adjuvant to conventional treatment returned better results in healing and lower recurrence rates after six months of intervention.


Assuntos
Úlcera Varicosa , Doença Crônica , Humanos , Lasers , Estudos Prospectivos , Úlcera Varicosa/terapia , Cicatrização
20.
J Biol Regul Homeost Agents ; 35(3 Suppl. 1): 29-35, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34289662

RESUMO

The case report aims to describe the parameters of performing upper labial frenectomy with the use of diode laser beams without infiltrated local anaesthesia. A 6-year-old patient was referred by the orthodontist for assessment of the upper anterior labial frenum. The dental treatment plan reported only the presence of caries on deciduous teeth and seals on the first permanent molars. The clinical examination reported the presence of a high attachments of labial frenum with a pathologically attachment and the presence of a diastema supports this theory. The laser used to remove the frenulum was a diode laser used with a wavelength of 980 nm with 320 microns of fiber in contact with a power of 2.0 W in continuous wave mode. The clinical examination showed an acceptable healing by secondary intention of the wound and the initial functional recovery of a physiological upper lip movements. The patient reported that the procedure was well tolerated. The diode laser can be used with good result for the removal of pathological frenum. The diode laser can be used in pediatric dentistry because of its application, adequate coagulation, no need for sutures and less inflammation and pain.


Assuntos
Freio Labial , Lasers Semicondutores , Anestesia Local , Criança , Humanos , Freio Labial/diagnóstico por imagem , Freio Labial/cirurgia , Lábio/diagnóstico por imagem , Lábio/cirurgia , Cicatrização
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