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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 284: 121779, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36041262

RESUMO

Online monitoring of antibiotics in the environment attracts more and more attention. Surface-enhanced Raman scattering (SERS) is a promising technique for the detection of trace amounts of antibiotics in the environment, which is fast, non-invasive and sensitive. To investigate the enrichment of trace amounts of antibiotics in water, polyethylene microplastics (PE MPs) were prepared as sorbents to simply concentrate enrofloxacin, ciprofloxacin hydrochloride monohydrate and triclosan in water, followed by the SERS measurement of antibiotics extract washed from MPs on an AgNPs@Si SERS substrate. Limit of detection of Rhodamine 6G is 2.1 × 10-12 M achieved from the AgNPs@Si SERS, indicating a high enhancement. The detection results show that SERS peaks of the antibiotics could be observed from the spectra of the extracts eluted from MPs, indicating MPs could adsorb and desorb antibiotics from water. Besides, for enrofloxacin and triclosan, the intensity of SERS measured from the MPs extracts are higher than that of directly from the spiked water, demonstrating the proposed method could lower the detectable concentration of hydrophobic antibiotics in water. Moreover, the proposed MPs sorbents combined with SERS method was applied to detect the antibiotics in real river water, with minimal detection of 10-10 M, 10-8 M, and 10-8 M achieved for enrofloxacin, ciprofloxacin hydrochloride monohydrate and triclosan, respectively. The proposed method provides a promising simple, rapid and low reagent consuming means for monitoring antibiotics in water.


Assuntos
Antibacterianos , Triclosan , Antibacterianos/análise , Ciprofloxacina , Enrofloxacina , Microplásticos , Plásticos , Polietilenos , Prata/química , Análise Espectral Raman/métodos , Tecnologia , Água
2.
J Enzyme Inhib Med Chem ; 38(1): 118-137, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36305290

RESUMO

A series of novel ciprofloxacin (CP) derivatives substituted at the N-4 position with biologically active moieties were designed and synthesised. 14 compounds were 1.02- to 8.66-fold more potent than doxorubicin against T-24 cancer cells. Ten compounds were 1.2- to 7.1-fold more potent than doxorubicin against PC-3 cancer cells. The most potent compounds 6, 7a, 7b, 8a, 9a, and 10c showed significant Topo II inhibitory activity (83-90% at 100 µM concentration). Compounds 6, 8a, and 10c were 1.01- to 2.32-fold more potent than doxorubicin. Compounds 6 and 8a induced apoptosis in T-24 (16.8- and 20.1-fold, respectively compared to control). This evidence was supported by an increase in the level of apoptotic caspase-3 (5.23- and 7.6-fold, sequentially). Both compounds arrested the cell cycle in the S phase in T-24 cancer cells while in PC-3 cancer cells the two compounds arrested the cell cycle in the G1 phase. Molecular docking simulations of compounds 6 and 8a into the Topo II active site rationalised their remarkable Topo II inhibitory activity.


Assuntos
Antineoplásicos , DNA Topoisomerases Tipo II , DNA Topoisomerases Tipo II/metabolismo , Ciprofloxacina/farmacologia , Ciprofloxacina/química , Simulação de Acoplamento Molecular , Linhagem Celular Tumoral , Relação Estrutura-Atividade , Estrutura Molecular , Relação Dose-Resposta a Droga , Antineoplásicos/química , Doxorrubicina/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Proliferação de Células
3.
J Colloid Interface Sci ; 630(Pt A): 544-555, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36270175

RESUMO

Efforts to develop a green, inexpensive and effective adsorbent are crucial for eliminating antibiotics in polluted water. The sorption capacity of the as-prepared polyvinyl alcohol (PVA)-assisted cellulose nanocrystals/SiO2 (CNCs/SiO2) composite aerogel to ciprofloxacin (CIP) rises with the increase of temperature and initial concentration. Reverse trend of sorption capacity can be found when increasing the adsorbent dosage of adsorbent. The optimal pH value for the sorption is proved to be 4. It's found in the uniaxial compression test that the maximum load that PVA-assisted aerogels can withstand is nearly 100 times than that of non-PVA aerogels. Sorption results confirm that the Pseudo-second order (R2 = 0.9885) and Langmuir models (R2 = 0.9959) fit well to sorption kinetics and equilibrium data, respectively. The rate constant differs from the initial concentration of CIP according to the Pseudo-second order model. The composite aerogel sorption capacity of Langmuir (qmax) for CIP was 163.34 mg·g-1. The thermodynamic studies showed that the sorption process is endothermic with the value of enthalpy change of 41.032 kJ/mol. Hydrogen bonding, π-π interaction, hydrophobic and electrostatic interactions are the dominant mechanisms of CIP sorption by the PVA-assisted CNCs/SiO2 composite aerogel.


Assuntos
Ciprofloxacina , Álcool de Polivinil , Ciprofloxacina/química , Álcool de Polivinil/química , Dióxido de Silício/química , Adsorção , Cinética , Concentração de Íons de Hidrogênio
4.
Food Chem ; 404(Pt B): 134757, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36327503

RESUMO

The abuse of enrofloxacin (ENR) in aquaculture and the lack of monitoring of other metabolites except ciprofloxacin (CIP) may lead to unknown harmful effects on human health. In this study, ENR metabolites were screened in real fish samples based on ultrahigh-performance liquid chromatography coupled with Q-Orbitrap mass spectrometry combined with Compound Discoverer software, and another metabolite deethylene-ENR besides CIP was detected and identified for the first time. Correspondingly, a method for the determination of ENR and CIP and the semi-quantitative analysis of deethylene-ENR in aquatic products was established. Method validation illustrated that excellent linearity and satisfactory recoveries of analytes were obtained. Limits of detection of ENR and CIP were both 0.1 µg kg-1, and their limits of quantification both 1 µg kg-1. CIP and deethylene-ENR were detected in 12 of 14 ENR-positive fish samples, so deethylene-ENR should be of concern as a possible risk candidate in aquatic products.


Assuntos
Ciprofloxacina , Fluoroquinolonas , Animais , Humanos , Enrofloxacina , Fluoroquinolonas/análise , Ciprofloxacina/análise , Cromatografia Líquida , Espectrometria de Massas , Cromatografia Líquida de Alta Pressão
5.
Food Chem ; 404(Pt B): 134761, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36332581

RESUMO

In this work, an ultrasensitive ciprofloxacin (CIP) detection strategy has been established based on copper (Cu2+) ions-induced strong charge transfer in poly acrylic acid (PAA) functionalized upconversion nanoparticles (UCNPs)/2,2-bipyridine (bipy) system. The positively charged Cu2+ ions electrostatically adhere to the surface of the PAA-UCNPs and deactivate the fluorescence via a charge transfer process. The bipy in this hybrid system controls the aggregation by chelating in proximity to the Cu2+ center. Due to the strong affinity between pyridone oxygen and carboxy oxygen, CIP coordinates in high stoichiometry with the bipy-Cu complex as compared to the PAA-UCNPs, causing the trapped fluorescence to be released in an amount equivalent to the target concentration. Under the optimum assay conditions, a good calibration plot (0.05-1000 ng/mL) was acquired with a detection limit of 0.13 ng/mL. The satisfactory recoveries (85.93-96.87%) for real prawn and fish samples were further validated by enzyme-linked immunoassays (P > 0.05).


Assuntos
Cobre , Nanopartículas , Animais , Transferência Ressonante de Energia de Fluorescência , Ciprofloxacina , Íons , Oxigênio
6.
Environ Res ; 216(Pt 3): 114716, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36336092

RESUMO

Microplastics (MPs) have recently become an emerging environmental concern. Nevertheless, limited information is known about the adsorption of MPs for organic contaminants under combined heavy metals pollution, with an emphasis on the role of complexation. Thus, this study aims to comprehensively compare and investigate the adsorption performance of antibiotic tetracycline (TC) and ciprofloxacin (CIP) on two polar MPs (polyamide (PA) and polyvinyl chloride (PVC)) affected by Cu(II) and Cd(II) with contrasting complexation abilities. Batch adsorption experiments were used in combination with speciation calculation, zeta potential determination, FTIR spectroscopy characterization and investigation of the affinity of MPs for heavy metals. Results showed that the sorption kinetics and isotherms of TC and CIP on PA and PVC could be well fitted to pseudo-second-order and Langmuir models, respectively, both in the absence and presence of Cu and Cd, suggesting that multiple interactions and monolayer adsorption played an important role in the adsorption process. The presence of Cu substantially improved TC and CIP adsorption and obviously changed the pH dependence of their adsorption onto both MPs, which may result from the Cu-induced strong complexation with TC and CIP. The presence of Cd slightly enhanced TC adsorption on both MPs while reduced CIP adsorption especially on PVC, which may be ascribed to the Cd-induced cationic bridging effects in TC adsorption and the competitive adsorption of Cd in CIP adsorption. Therefore, the heavy metal-mediated complexation effects may play a dominant role in antibiotic adsorption by MPs only in the presence of heavy metals with strong complexation ability while the adsorption performance in the presence of heavy metals with negligible complexation capacity may be influenced by effects other than complexation. This study helps further understand the heavy metal-mediated adsorption behavior of organic contaminants on polar MPs and the role of complexation reactions therein.


Assuntos
Metais Pesados , Poluentes Químicos da Água , Microplásticos , Adsorção , Ciprofloxacina/química , Plásticos , Antibacterianos , Cádmio , Cloreto de Polivinila , Metais Pesados/química , Tetraciclina/química , Poluentes Químicos da Água/análise
7.
Environ Res ; 216(Pt 1): 114437, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36181898

RESUMO

Pharmaceutical compounds being able to alter, retard, and enhance metabolism has gained attention in recent time as emerging pollutant. However, hospitals which are part of every urban landscape have yet to gain attention in terms of its hospital wastewater treatment to inhibit pharmaceutical compounds from reaching environment. Hence this study evaluated performance of constructed wetland in combination with tubesettler and aeration based on removal efficiency and ecological risk assessment (HQ). The removal efficiency of constructed wetland with plantation was higher by 31% (paracetamol), 102% (ibuprofen), 46%, (carbamazepine), 57% (lorazepam), 54% (erythromycin), 31% (ciprofloxacin) and 20% (simvastatin) against constructed wetland without plantation. Constructed wetland with aeration efficiency increased for paracetamol, ibuprofen, carbamazepine, lorazepam, erythromycin, ciprofloxacin, and simvastatin removal efficiency were higher by 58%, 130%, 52%, 79%, 107%, 57%, and 29% respectively. In constructed wetland with plantation, removal efficiency was higher by 20% (paracetamol), 13% (ibuprofen), 4% (carbamazepine), 14% (lorazepam), 34% (erythromycin), 19% (ciprofloxacin) and 7% (simvastatin). High ecological risk was observed for algae, invertebrate and fish with hazard quotient values in range of 2.5-484, 10-631 and 1-78 respectively. This study concludes that if space is the limitation at hospitals aeration with constructed wetland can be adopted. If space is available, constructed wetland with tubesettler is suitable, economic and environmentally friendly option. Future research works can focus on evaluating other processes combination with constructed wetland.


Assuntos
Águas Residuárias , Áreas Alagadas , Animais , Águas Residuárias/análise , Eliminação de Resíduos Líquidos , Ibuprofeno , Acetaminofen , Lorazepam , Carbamazepina , Hospitais , Ciprofloxacina , Eritromicina , Sinvastatina , Preparações Farmacêuticas
8.
Carbohydr Polym ; 300: 120272, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36372494

RESUMO

The aim of this study was to design a pectin-chitosan (PEC-CS) hydrogel loaded with a bioadhesive-design micelle containing large amount of ciprofloxacin for antibacterial and healing wound applications. Pectin and chitosan are crosslinked in a safe and convenient way, and the PEC-CS hydrogel have high water content (>95 %), strong water absorption (15,000 %), good water retention (>10,000 % at 30 % RH for 12 h), and the PEC-CS hydrogels showed no cytotoxicity and hemolysis, thus providing a humid microenvironment suitable for wound. Additionally, the dopamine modified carrier can greatly improve the solubility and retention time in the wound of ciprofloxacin, effectively increase the efficiency of drug loading into the PEC-CS hydrogels and exert antibacterial activity in the wound for a long time. In vitro and in vivo pharmacodynamics experiments have shown that PEC-CS#CIP@DPDMCs hydrogels can resist bacteria and promote wound healing. Thus,The PEC-CS#CIP@DPDMCs hydrogels can be a potential anti-infective hydrogel excipient.


Assuntos
Infecções Bacterianas , Quitosana , Humanos , Hidrogéis/farmacologia , Pectinas/farmacologia , Micelas , Cicatrização , Ciprofloxacina , Antibacterianos/farmacologia , Água
9.
Environ Res ; 216(Pt 2): 114568, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36252840

RESUMO

Developing efficient and stable visible light active photocatalyst has significant environmental applications. Though dye sensitization of TiO2 nanoparticles with natural chlorophyll pigments can potentially impart visible light activity, their long-term stability is a major concern. We investigated the functionalization of TiO2 with salicylic acid, and subsequent sensitization with chlorophylls to improve the catalyst stability for the photocatalytic degradation of Ciprofloxacin (CPX) under visible light. A significant improvement in the degradation efficiency and catalyst stability was observed for five reuse cycles. Further, an optimum CPX degradation of ∼75% was achieved with 0.75 g L-1 catalyst dosage of 0.1 chl/0.1 SA-TiO2, initial pH of 6, and 10 ppm of initial CPX for a visible light exposure of 2 h. The degradation followed the pseudo-second-order kinetics. In addition, the ciprofloxacin degradation was reduced in the wastewater matrix system due to the presence of other scavenging species such as chlorides, sulphates, and alkalinity. Significant reduction in the toxicity of degradation compounds after the photocatalytic degradation was observed in comparison to parent CPX. Further, the degradation pathway and plausible mechanism of degradation of CPX were also proposed.


Assuntos
Nanopartículas , Poluentes Químicos da Água , Ciprofloxacina , Clorofila , Ácido Salicílico , Poluentes Químicos da Água/análise , Titânio/química , Catálise , Luz
10.
Bioorg Chem ; 130: 106266, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36399865

RESUMO

The antibiotic crisis is associated with the appearance of multidrug resistant (MDR) pathogens, which has caused severe bacterial infections and imposed a huge burden on modern society. Therefore, there is an urgent need to develop new antibacterial drugs with novel mechanism of action. Here we designed and synthesized three series of benzoxazolone, oxazolopyridinone and 3-(2-hydroxyphenyl)hydantoin derivatives and evaluated their activity as novel quorum sensing (QS) inhibitors. We found that benzoxazolone and oxazolopyridinone derivatives had promising QS inhibitory activity in the minimum inhibitory concentration, pyocyanin and rhamnolipid inhibition assays. In particular, A10 and B20 at 256 µg/mL not only suppressed pyocyanin production regulated by QS in P. aeruginosa PAO1 by 36.55% and 46.90%, respectively, but also showed the strongest rhamnolipid inhibitory activity with the IC50 values of 66.35 and 56.75 µg/mL, respectively. Further studies demonstrated that B20 at 64 µg/mL inhibited biofilm formation in P. aeruginosa PAO1 by 40%, and weakened its swarming motility. More importantly, the bacterial mortality of B20 combined with ciprofloxacin and clarithromycin against P. aeruginosa were 48.27% and 49.79%, respectively, while ciprofloxacin and clarithromycin had only 16.99% and 29.11% of bacterial mortality against P. aeruginosa when used alone. Mechanistic studies indicated that B20 directly inhibited the QS pathway based on the GFP reporter strain assay. Overall, this compound with oxazolopyridinone core could serve as an antibacterial lead of QS inhibitor for further evaluation of its drug-likeness.


Assuntos
Piocianina , Percepção de Quorum , Claritromicina , Pseudomonas aeruginosa , Ciprofloxacina , Antibacterianos/farmacologia
11.
J Hazard Mater ; 442: 130070, 2023 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-36183515

RESUMO

Advanced oxidation processes (AOP) are a common tool to remove organic compounds from the water cycle. The process is mostly relied on free radicals (i.e., SO4•- and HO•) with high oxidation power in solution. Surface-mediated mechanism could improve this process to prevent undesired quenching of aqueous radicals that widely exists in free radical pathways and alleviate metal leaching through direct electron transfer. In this work, a facile low-temperature pre-treatment combined with pyrolytic strategy was employed to construct a green catalyst with iron oxides embedded in Kraft-lignin derived bio-char (γ-Fe2O3 @KC), upon which radicals stay surface mediated and the activity-stability trade-off is achieved for pollutant degradation. The γ-Fe2O3 @KC is capable of activating PMS to generate non-radical species which are more stable (1O2 and Fe(V)=O) and of enhancing electron transfer efficiency. A surface-bound reactive complex (Catalyst-PMS*) was identified by electrochemical characterization and was discussed with primary surface-bound radical pairs to explain the contradictions between quenching and EPR detection results. We analyzed the γ-Fe2O3 @KC as a PMS-activating catalyst for a wider range of oxidation targets, such as Rhodamine B (∼100%), p-nitrophenol (∼85%), and Ciprofloxacin (∼63%), and found competitive removal efficiencies. The system also shows an encouraging reusability for at least 5 times and high stability at pH 3-9, and the low concentration of iron in γ-Fe2O3 @KC/PMS system implies the carbon scaffold of biochar alleviate the leakage process. The combined findings highlight the applicability in 'green (source) to green (application)' processes using cost-effective and bio-friendly iron@carbon catalysts, where alternative oxidation pathways are activated to play a dominant role for water purification.


Assuntos
Carbono , Poluentes Ambientais , Carbono/química , Água , Peróxidos/química , Lignina , Ferro , Radicais Livres , Ciprofloxacina
12.
PLoS One ; 17(11): e0277574, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36409683

RESUMO

Antimicrobial resistance against shigellosis is increasingly alarming. However, evidence-based knowledge gaps regarding the changing trends of shigellosis in Bangladesh exist due to the scarcity of longitudinal data on antimicrobial resistance. Our study evaluated the last 20 years antimicrobial resistance patterns against shigellosis among under-5 children in the urban and rural sites of Bangladesh. Data were extracted from the Diarrheal Disease Surveillance System (DDSS) of Dhaka Hospital (urban site) and Matlab Hospital (rural site) of the International Centre for Diarrheal Disease Research, Bangladesh (icddr,b) between January 2001 and December 2020. We studied culture-confirmed shigellosis cases from urban Dhaka Hospital (n = 883) and rural Matlab Hospital (n = 1263). Since 2001, a declining percentage of shigellosis in children observed in urban and rural sites. Moreover, higher isolation rates of Shigella were found in the rural site [1263/15684 (8.1%)] compared to the urban site [883/26804 (3.3%)] in the last 20 years. In both areas, S. flexneri was the predominant species. The upward trend of S. sonnei in both the study sites was statistically significant after adjusting for age and sex. WHO-recommended 1st line antibiotic ciprofloxacin resistance gradually reached more than 70% in both the urban and rural site by 2020. In multiple logistic regression after adjusting for age and sex, ciprofloxacin, azithromycin, mecillinam, ceftriaxone, and multidrug resistance (resistance to any two of these four drugs) among under-5 children were found to be increasing significantly (p<0.01) in the last 20 years in both sites. The study results underscore the importance of therapeutic interventions for shigellosis by appropriate drugs based on their current antibiogram for under-5 children. These observations may help policymakers in formulating better case management strategies for shigellosis.


Assuntos
Disenteria Bacilar , Criança , Humanos , Disenteria Bacilar/tratamento farmacológico , Disenteria Bacilar/epidemiologia , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Bangladesh/epidemiologia , Farmacorresistência Bacteriana , Diarreia/epidemiologia , Hospitais Rurais , Ciprofloxacina/uso terapêutico
13.
Molecules ; 27(21)2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36364007

RESUMO

Resin composites have been widely used in dental restoration. However, polymerization shrinkage and resultant bacterial microleakage are major limitations that may lead to secondary caries. To overcome this, a new type of antibacterial resin composite containing ciprofloxacin-loaded silver nanoparticles (CIP-AgNPs) were synthesized. The chemical reduction approach successfully produced CIP-AgNPs, as demonstrated by FTIR, zeta potential, scanning electron microscopy, and ultraviolet-visible (UV-vis) spectroscopy. CIP-AgNPs were added to resin composites and the antibacterial activity of the dental composite discs were realized against Enterococcus faecalis, Streptococcus mutans, and the Saliva microcosm. The biocompatibility of modified resin composites was assessed and mechanical testing of modified dental composites was also performed. The results indicated that the antibacterial activity and compressive strength of resin composites containing CIP-AgNPs were enhanced compared to the control group. They were also biocompatible when compared to resin composites containing AgNPs. In short, these results established strong ground application for CIP-AgNP-modified dental composite resins.


Assuntos
Nanopartículas Metálicas , Nanopartículas , Prata/farmacologia , Prata/química , Ciprofloxacina/farmacologia , Streptococcus mutans , Antibacterianos/farmacologia , Antibacterianos/química , Resinas Compostas/farmacologia , Resinas Compostas/química , Teste de Materiais , Nanopartículas/química
14.
Sci Rep ; 12(1): 20319, 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36434075

RESUMO

The emergence of antibiotic-resistance genes (ARGs) by means of integrons in multidrug-resistant Acinetobacter baumannii (MDR A. baumannii) has become a significant challenge in the management of infections from this pathogen. In this paper, we report on the variable region of class 1 and 2 integrons observed in MDR A. baumanni isolates recovered from rivers in the Eastern Cape Province, South Africa. Class 1 and 2 integrons with their variable regions were evaluated with polymerase chain reaction techniques followed by sequencing. Antibiotic sensitivity testing, checkerboard assay, time-kill independent assay, and Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) were carried out using standard microbiological techniques. A total of fifty-six (56) isolates were examined, among which 45 (79%) tested positive for class 1 integron, and 7 (12.3%) had class 2 integron. None was found to be class 3 integron positive among the isolates. The variable region contained aadA1, aadA5, and aadA2 genes, which confer resistance against streptomycin and spectinomycin, aac(6')-Ib against amikacin/ tobramycin and dfrA17 genes against trimethoprim. The minimum inhibitory concentrations of the antimicrobials for one of the tested organisms were resistant against meropenem, colistin sodium methanesulfonate, tetracycline, ceftazidime, and ciprofloxacin (16, > 16, > 8, > 256, and 128 ug/mL respectively). The impact of colistin combined with quinolones (ciprofloxacin), with the FICIs (0.31) indicated synergistic effects against MDR A baumanni. However, when colistin was combined with meropenem and ceftazidime, additive effects with fractional inhibitory concentration (FIC) index ranging from 0.52 to 1 were observed. No antagonistic effect was evaluated among the examined isolates. ERIC-PCR analyses of A. baumanni isolates revealed significant genetic diversity, suggesting various sources of environmental contamination. We conclude that A. baumanni harbouring class 1 integrons in aquatic milieus are a significant source of ARGs and can transmit these elements to other organisms and consequently to man with significant public health implications.


Assuntos
Acinetobacter baumannii , Integrons , Humanos , Masculino , Integrons/genética , Colistina , Prevalência , Meropeném , Ceftazidima , África do Sul , Antibacterianos/farmacologia , Ciprofloxacina , Acinetobacter baumannii/genética , Enterobacteriaceae
15.
Curr Microbiol ; 80(1): 5, 2022 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-36434296

RESUMO

Lungs of cystic fibrosis patients are often colonized or infected with organisms, such as Pseudomonas aeruginosa and other emerging pathogenic bacteria such as Achromobacter xylosoxidans. Further, it is well established that infections of the cystic fibrosis lung airways are caused by polymicrobial infections, although its composition and diversity may change throughout the patient's life. In the present study, we investigated the effects of N-acetylcysteine (NAC) and amikacin, aztreonam, ciprofloxacin, and tobramycin alone and in combination against single- and dual-species biofilms of P. aeruginosa and A. xylosoxidans, in vitro and in the Caenorhabditis elegans infection model. Results showed that tobramycin and ciprofloxacin were the most effective antibiotics, while aztreonam was the least effective antibiotic against both single- and dual-species biofilms of P. aeruginosa and A. xylosoxidans. However, NAC showed little effect on both single- and dual-species, even with a combination of antibiotics. Increased survival was observed in C. elegans when treated with NAC in combination with tobramycin or ciprofloxacin, compared to no treatment or NAC alone. Tobramycin and ciprofloxacin were found effective in biofilms, but more research is needed to better understand the effects of NAC and antibiotics against single- and dual-species biofilms.


Assuntos
Achromobacter denitrificans , Fibrose Cística , Animais , Humanos , Pseudomonas aeruginosa , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Acetilcisteína/farmacologia , Aztreonam/farmacologia , Fibrose Cística/microbiologia , Caenorhabditis elegans , Biofilmes , Tobramicina/farmacologia , Ciprofloxacina/farmacologia
16.
Huan Jing Ke Xue ; 43(11): 4982-4991, 2022 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-36437070

RESUMO

In recent years, the abuse of antibiotics has led to the spread and diffusion of antibiotic resistance genes in the environment, which poses a potential threat to the ecosystem and human health. In particular, the related reports of antibiotic contamination in drinking water have aroused great social concerns. Therefore, realizing the rapid detection of trace antibiotics in emergency events has become a research hotspot. Here, in combination with magnetic solid phase extraction (MSPE), we established a rapid detection strategy for ng·L-1 level quinolones in drinking water using surface-enhanced Raman spectroscopy (SERS). With the help of the high enrichment capacity provided by the high adsorption capacity of the magnetic graphene oxide composite nanomaterial (Fe3O4@SiO2-GO), the spiked detection of 1.0 ng·L-1 enrofloxacin (ENR) and 5.0 ng·L-1 ciprofloxacin (CIP) in drinking water was successfully achieved, with recoveries ranging from 77.5% to 91.5%, which met the current requirements of drinking water testing. For environmental water samples such as lake water, the selectivity of extraction materials needs to be further improved due to the strong interference of the complex organic matrix.


Assuntos
Água Potável , Poluentes Químicos da Água , Humanos , Ciprofloxacina , Enrofloxacina , Ecossistema , Dióxido de Silício , Poluentes Químicos da Água/análise , Antibacterianos/análise
17.
Int J Mol Sci ; 23(22)2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36430740

RESUMO

Cu2V2O7/Cu3V2O8/g-C3N4 heterojunctions (CVCs) were prepared successfully by the reheating synthesis method. The thermal etching process increased the specific surface area. The formation of heterojunctions enhanced the visible light absorption and improved the separation efficiency of photoinduced charge carriers. Therefore, CVCs exhibited superior adsorption capacity and photocatalytic performance in comparison with pristine g-C3N4 (CN). CVC-2 (containing 2 wt% of Cu2V2O7/Cu3V2O8) possessed the best synergistic removal efficiency for removal of dyes and antibiotics, in which 96.2% of methylene blue (MB), 97.3% of rhodamine B (RhB), 83.0% of ciprofloxacin (CIP), 86.0% of tetracycline (TC) and 80.5% of oxytetracycline (OTC) were eliminated by the adsorption and photocatalysis synergistic effect under visible light irradiation. The pseudo first order rate constants of MB and RhB photocatalytic degradation on CVC-2 were 3 times and 10 times that of pristine CN. For photocatalytic degradation of CIP, TC and OTC, it was 3.6, 1.8 and 6.1 times that of CN. DRS, XPS VB and ESR results suggested that CVCs had the characteristics of a Z-scheme photocatalytic system. This study provides a reliable reference for the treatment of real wastewater by the adsorption and photocatalysis synergistic process.


Assuntos
Poluentes Ambientais , Oxitetraciclina , Adsorção , Tetraciclina , Ciprofloxacina , Antibacterianos , Azul de Metileno
18.
Molecules ; 27(22)2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36431831

RESUMO

Four new 2-3D materials were designed and synthesized by hydrothermal methods, namely, {[(L1·Cu·2H2O) (4,4-bipy)0.5] (ß-Mo8O26)0.5·H2O} (1), {[(L1·Cu)2·(4,4-bipy)] (Mo5O16)} (2), {Co(L1)2}n&nbsp;(3), and {[(L1)2][ß-Mo8O26]0.5·5H2O} (4). [L1=5-(4-aminopyridine) isophthalic acid]. The degradation of ciprofloxacin (CIP) in water by compounds 1-4 was studied under visible light. The experimental results show that compounds 1-4 have obvious photocatalytic degradation effect on CIP. In addition, for compound 1, the effects of temperature, pH, and adsorbent dosage on photocatalytic performance were also investigated. The stability of compound 1 was observed by a cycle experiment, indicating that there was no significant change after three cycles of CIP degradation.


Assuntos
Ácidos Carboxílicos , Ciprofloxacina , Polímeros , Temperatura , Água
19.
Cochrane Database Syst Rev ; 11: CD010452, 2022 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-36420914

RESUMO

BACKGROUND: Typhoid and paratyphoid (enteric fever) are febrile bacterial illnesses common in many low- and middle-income countries. The World Health Organization (WHO) currently recommends treatment with azithromycin, ciprofloxacin, or ceftriaxone due to widespread resistance to older, first-line antimicrobials. Resistance patterns vary in different locations and are changing over time. Fluoroquinolone resistance in South Asia often precludes the use of ciprofloxacin. Extensively drug-resistant strains of enteric fever have emerged in Pakistan. In some areas of the world, susceptibility to old first-line antimicrobials, such as chloramphenicol, has re-appeared. A Cochrane Review of the use of fluoroquinolones and azithromycin in the treatment of enteric fever has previously been undertaken, but the use of cephalosporins has not been systematically investigated and the optimal choice of drug and duration of treatment are uncertain. OBJECTIVES: To evaluate the effectiveness of cephalosporins for treating enteric fever in children and adults compared to other antimicrobials. SEARCH METHODS: We searched the Cochrane Infectious Diseases Group Specialized Register, CENTRAL, MEDLINE, Embase, LILACS, the WHO ICTRP and ClinicalTrials.gov up to 24 November 2021. We also searched reference lists of included trials, contacted researchers working in the field, and contacted relevant organizations. SELECTION CRITERIA: We included randomized controlled trials (RCTs) in adults and children with enteric fever that compared a cephalosporin to another antimicrobial, a different cephalosporin, or a different treatment duration of the intervention cephalosporin. Enteric fever was diagnosed on the basis of blood culture, bone marrow culture, or molecular tests. DATA COLLECTION AND ANALYSIS: We used standard Cochrane methods. Our primary outcomes were clinical failure, microbiological failure and relapse. Our secondary outcomes were time to defervescence, duration of hospital admission, convalescent faecal carriage, and adverse effects. We used the GRADE approach to assess certainty of evidence for each outcome. MAIN RESULTS: We included 27 RCTs with 2231 total participants published between 1986 and 2016 across Africa, Asia, Europe, the Middle East and the Caribbean, with comparisons between cephalosporins and other antimicrobials used for the treatment of enteric fever in children and adults. The main comparisons are between antimicrobials in most common clinical use, namely cephalosporins compared to a fluoroquinolone and cephalosporins compared to azithromycin. Cephalosporin (cefixime) versus fluoroquinolones Clinical failure, microbiological failure and relapse may be increased in patients treated with cefixime compared to fluoroquinolones in three small trials published over 14 years ago: clinical failure (risk ratio (RR) 13.39, 95% confidence interval (CI) 3.24 to 55.39; 2 trials, 240 participants; low-certainty evidence); microbiological failure (RR 4.07, 95% CI 0.46 to 36.41; 2 trials, 240 participants; low-certainty evidence); relapse (RR 4.45, 95% CI 1.11 to 17.84; 2 trials, 220 participants; low-certainty evidence). Time to defervescence in participants treated with cefixime may be longer compared to participants treated with fluoroquinolones (mean difference (MD) 1.74 days, 95% CI 0.50 to 2.98, 3 trials, 425 participants; low-certainty evidence). Cephalosporin (ceftriaxone) versus azithromycin Ceftriaxone may result in a decrease in clinical failure compared to azithromycin, and it is unclear whether ceftriaxone has an effect on microbiological failure compared to azithromycin in two small trials published over 18 years ago and in one more recent trial, all conducted in participants under 18 years of age: clinical failure (RR 0.42, 95% CI 0.11 to 1.57; 3 trials, 196 participants; low-certainty evidence); microbiological failure (RR 1.95, 95% CI 0.36 to 10.64, 3 trials, 196 participants; very low-certainty evidence). It is unclear whether ceftriaxone increases or decreases relapse compared to azithromycin (RR 10.05, 95% CI 1.93 to 52.38; 3 trials, 185 participants; very low-certainty evidence). Time to defervescence in participants treated with ceftriaxone may be shorter compared to participants treated with azithromycin (mean difference of -0.52 days, 95% CI -0.91 to -0.12; 3 trials, 196 participants; low-certainty evidence). Cephalosporin (ceftriaxone) versus fluoroquinolones It is unclear whether ceftriaxone has an effect on clinical failure, microbiological failure, relapse, and time to defervescence compared to fluoroquinolones in three trials published over 28 years ago and two more recent trials: clinical failure (RR 3.77, 95% CI 0.72 to 19.81; 4 trials, 359 participants; very low-certainty evidence); microbiological failure (RR 1.65, 95% CI 0.40 to 6.83; 3 trials, 316 participants; very low-certainty evidence); relapse (RR 0.95, 95% CI 0.31 to 2.92; 3 trials, 297 participants; very low-certainty evidence) and time to defervescence (MD 2.73 days, 95% CI -0.37 to 5.84; 3 trials, 285 participants; very low-certainty evidence). It is unclear whether ceftriaxone decreases convalescent faecal carriage compared to the fluoroquinolone gatifloxacin (RR 0.18, 95% CI 0.01 to 3.72; 1 trial, 73 participants; very low-certainty evidence) and length of hospital stay may be longer in participants treated with ceftriaxone compared to participants treated with the fluoroquinolone ofloxacin (mean of 12 days (range 7 to 23 days) in the ceftriaxone group compared to a mean of 9 days (range 6 to 13 days) in the ofloxacin group; 1 trial, 47 participants; low-certainty evidence). AUTHORS' CONCLUSIONS: Based on very low- to low-certainty evidence, ceftriaxone is an effective treatment for adults and children with enteric fever, with few adverse effects. Trials suggest that there may be no difference in the performance of ceftriaxone compared with azithromycin, fluoroquinolones, or chloramphenicol. Cefixime can also be used for treatment of enteric fever but may not perform as well as fluoroquinolones.  We are unable to draw firm general conclusions on comparative contemporary effectiveness given that most trials were small and conducted over 20 years previously. Clinicians need to take into account current, local resistance patterns in addition to route of administration when choosing an antimicrobial.


Assuntos
Anti-Infecciosos , Febre Paratifoide , Febre Tifoide , Criança , Adulto , Humanos , Adolescente , Febre Paratifoide/tratamento farmacológico , Febre Tifoide/tratamento farmacológico , Cefalosporinas/uso terapêutico , Azitromicina/efeitos adversos , Ceftriaxona/uso terapêutico , Cefixima/uso terapêutico , Fluoroquinolonas/uso terapêutico , Antibacterianos/uso terapêutico , Cloranfenicol/uso terapêutico , Anti-Infecciosos/uso terapêutico , Monobactamas/uso terapêutico , Ciprofloxacina/uso terapêutico , Ofloxacino/uso terapêutico , Recidiva , Paquistão
20.
Investig Clin Urol ; 63(6): 663-670, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36347556

RESUMO

PURPOSE: To assess the effect of ciprofloxacin (CP) and fosfomycin compared with CP and amikacin in patients with a fluoroquinolone (FQ)-resistant rectal flora who have undergone transrectal ultrasound-guided prostate biopsy (TRUSPB). MATERIALS AND METHODS: In total, 516 patients with FQ-resistant rectal flora based on rectal swab cultures were divided into two groups according to prophylactic antibiotics. Patients in both groups were administered CP (400 mg, intravenous [IV], twice daily) on the same day as TRUSPB and 1 day after biopsy. The amikacin group (n=260) was administered a single injection of amikacin (1 g, IV) 1 hour before TRUSPB, whereas the fosfomycin group (n=256) was administered fosfomycin (3 g, orally) the night before the procedure. The primary endpoint was the rate of infectious complications in the two groups. RESULTS: Overall, 13 patients (2.5%) reported infectious complications: 12 patients (4.62%) in the amikacin group compared with 1 patient (0.39%) in the fosfomycin group (risk ratio, 0.09; 95% confidence interval [CI], 0.01-0.65), respectively, which was a statistically significant difference (p=0.017). This corresponds to a number needed to treat of 24 patients (95% CI, 15-65) to prevent one infectious complication. In the multivariate analysis to assess variables related to infectious complications, prophylactic antibiotics with added fosfomycin was associated with infectious complications (odds ratio, 0.060; 95% CI, 0.008-0.459). CONCLUSIONS: In the era of FQ resistance, CP and fosfomycin may reduce the rate of infectious complications compared with CP and amikacin prophylaxis.


Assuntos
Fosfomicina , Masculino , Humanos , Fosfomicina/uso terapêutico , Antibioticoprofilaxia/métodos , Ciprofloxacina/uso terapêutico , Próstata/patologia , Amicacina/uso terapêutico , Estudos Retrospectivos , Antibacterianos/uso terapêutico , Biópsia/métodos , Fluoroquinolonas
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