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1.
J Colloid Interface Sci ; 605: 766-778, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34371422

RESUMO

The reasonable design of the structure and composition of catalysts was essential to improve the catalytic performance of advanced oxidation processes (AOPs). Herein, we reported a simple strategy to synthesize hierarchical Co3O4-C@CoSiOx yolk-shell nanoreactors with multiple active components by using metal-organic frameworks (MOFs). The novel nanoreactors are further used to activate peroxymonosulfate (PMS) for ciprofloxacin (CIP) degradation. The effects of reaction parameters (pH value, co-existing ions, reaction temperature, etc.) on CIP degradation were systematically investigated. Especially, ∼98.2% of CIP was degraded within 17 min under the optimal conditions, together with the low cobalt leaching and excellent reusability. The appreciable catalytic performance improvement might be due to the synergistic effect of the structure and component design: (1) the hierarchical yolk-shell structure endowed the catalyst with high surface area (∼232.47 m2/g) and fully exposed active sites; (2) abundant highly active ≡Co-OH+ were formed on the surface of CoSiOx; (3) the presence of oxygen vacancies and nitrogen-doped carbon promoted the decomposition of PMS through a non-radical process. The results revealed both the radical (SO4∙-, ∙OH and O2∙-) and non-radical (1O2 and direct charge transfer) should be responsible for the CIP degradation. Moreover, the possible degradation pathways of CIP were proposed through the identification of intermediates using LC-MS/MS techniques and density functional theory (DFT) calculation. Our work highlights that multi-component catalysts derived from MOFs with novel structure have broad application prospects in AOPs.


Assuntos
Ciprofloxacina , Espectrometria de Massas em Tandem , Cromatografia Líquida , Nanotecnologia , Peróxidos
2.
J Colloid Interface Sci ; 605: 813-827, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34371426

RESUMO

The rapid evolution of antimicrobial resistant genes (AMRs) in water resources is well correlated to the persistent occurrence of ciprofloxacin in water. For the first time, encapsulated nanoscale zerovalent iron (nZVI) with a shell of magnesium hydroxide (Mg/Fe0) was used to adsorb ciprofloxacin from water. Optimization of the removal conditions exhibited that 5% was the optimum mass ratio between magnesium hydroxide and nZVI [Mg(OH)2/nZVI)] as more than 96% of 100 mg L-1 of ciprofloxacin was removed. In addition, 0.5 g L-1 of Mg/Fe0 showed an extraordinary performance in removing ciprofloxacin over a wide range of pH (3-11) with removal efficiencies exceeded 90%. Kinetic analysis displayed that the kinetic data was well described by both Pseudo first-order and second-order models. Also, the equilibrium data was well fitted by Freundlich isotherm model. In addition, thermodynamic analysis evidenced that the removal of ciprofloxacin by Mg/Fe0 was exothermic, and spontaneous. The experiments also revealed that physisorption and chemisorption were the responsible mechanisms for ciprofloxacin removal. The proposed treatment system remediated 10 litters of 100 mg L-1 of ciprofloxacin solution with 100% overall removal efficiency. This treatment system could be a promising and practical solution to decrease ciprofloxacin concentration in different water bodies.


Assuntos
Nanopartículas , Poluentes Químicos da Água , Purificação da Água , Adsorção , Ciprofloxacina , Ferro , Cinética , Hidróxido de Magnésio , Água , Poluentes Químicos da Água/análise
3.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120343, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34500409

RESUMO

Lipophilicity plays a significant role in the permeability of the drugs through cell membranes and impacts the drug activity in the human body. In this paper, the spectrophotometric method was used to determine the apparent partition coefficients of two amphoteric drugs: ciprofloxacin and levofloxacin. The apparent partition coefficient was determined with the classic shake-flask method with n-octanol according to OECD guidelines. The lipophilicity profiles in a wide range of pH were determined and described quantitatively with the quadratic function. Basing on the macro- and microdissociation constants, the true partition coefficient for both drugs was calculated. Both levofloxacin and ciprofloxacin were lipophilic. The neutral forms, i.e., zwitterionic and uncharged, dominate in the pH relevant to the one in the intestines, the place from which they are absorbed.


Assuntos
Ciprofloxacina , Levofloxacino , 1-Octanol , Humanos , Concentração de Íons de Hidrogênio , Permeabilidade , Solubilidade , Água
4.
J Colloid Interface Sci ; 606(Pt 2): 1715-1728, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34500170

RESUMO

The major challenges of clean energy and environmental pollution have resulted in the development of photocatalysis technologies for energy conversion and the degradation of refractory pollutants. Herein, a novel CdSe/Se/BiOBr hydrangea-like photocatalyst was used to produce hydrogen peroxide (H2O2) and degrade ciprofloxacin (CIP). The Z-scheme heterojunction structure of the photocatalyst and the doping of selenium (Se) led to the efficient separation of electron-hole pairs and charge transfer. The optimized sample of 2 wt% CdSe/Se/BiOBr produced 142.15 mg·L-1 rate of H2O2, which was much higher than that produced by pure BiOBr (89.4 mg·L-1) or CdSe/Se (10.9 mg·L-1). Additionally, almost 100 % of CIP was degraded within 30 min, with a first order rate constant of nearly 5.35 times that of pure BiOBr and 81.44 times that of pure CdSe/Se. The excellent removal efficiency of CIP from natural water matrices confirmed that the composites are promising for the removal of contaminants from natural waterways. Based on trapping experiments, electron spin resonance spectra (ESR) spectroscopy, and density functional theory (DFT) calculations, the photocatalytic mechanisms of H2O2 and CIP degradation by the Z-scheme CdSe/Se/BiOBr composites were proposed. Overall, the dual-functional CdSe/Se/BiOBr composite could potentially be applied for photocatalytic production of H2O2 and treatment of organic pollutants in water.


Assuntos
Compostos de Cádmio , Compostos de Selênio , Selênio , Bismuto , Catálise , Ciprofloxacina , Peróxido de Hidrogênio
5.
Chemosphere ; 287(Pt 4): 132391, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34597627

RESUMO

In this work, a novel BiOCl/Cu-doped Bi2S3 photocatalyst was designed to efficiently remove ciprofloxacin (CIP) with high photocatalytical activity and good stability over a wide pH range. Compared with Cu-doped Bi2S3, Bi2S3, BiOCl, BiOCl/Bi2S3, and Cu-doped BiOCl, the photocatalytical degradation rate of CIP (97.1% at 20 mg/L) over BiOCl/Cu-doped Bi2S3 was enhanced by about 84.77, 44.23, 2.95, 2.27, and 1.96 times within 20 min, respectively. Notably, the BiOCl/Cu-doped Bi2S3 photocatalyst also displayed high photocatalytical performance in the degradation of other antibiotics including norfloxacin, ofloxacin, and tetracycline (40 mL, 20 mg/L; 88.3%, 100%, and 95.2% of degradation rate within 30 min, respectively) under visible light irradiation. Radical trapping experiments and electron spin resonance technique indicated that superoxide radicals (•O2-) and photogenerated holes (h+) played crucial roles in the photocatalytic degradation of CIP. Finally, the possible CIP degradation pathways was proposed by detecting the CIP intermediates in photocatalytical reaction process.


Assuntos
Ciprofloxacina , Tetraciclina , Antibacterianos , Catálise , Norfloxacino
6.
J Environ Manage ; 301: 113765, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34592665

RESUMO

The central composite rotatable design (CCD) of response surface methodology (RSM) was used to optimize aluminum dispersed bamboo activated carbon preparation. The independent variables selected for optimization are activating agent (AlCl3) concentration (mol/L), activation temperature (°C), and activation time (min.). The independent variable's response change was observed through the percentage adsorption efficiency of Ciprofloxacin hydrochloride (CIP) antibiotics. The maximum CIP adsorption efficiency was found to be 93.6 ± 0.36% (13.36 mg/g) for the adsorbent prepared at AlCl3 concentration 2.0 mol/L, activation temperature 900 °C, and activation time 120 min. The adsorption efficiency was recorded at the natural pH (7.9) of the adsorbent (3 g/L)-adsorbate (50 mL solution of 50 ppm) mixture. The Al-dispersed bamboo activated carbon was characterized for its surface morphology, surface elemental compositions, molecular crystallinity, surface area, pore morphology, and surface functional groups. The mechanism of adsorbent surface formation and CIP adsorption sites were explored. The characterization data and mechanism study will help in deciding possible future applications in other fields of study.


Assuntos
Ciprofloxacina , Poluentes Químicos da Água , Adsorção , Alumínio , Antibacterianos , Carvão Vegetal , Ciprofloxacina/análise , Cinética , Poluentes Químicos da Água/análise
7.
Sci Total Environ ; 803: 150108, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34525766

RESUMO

The anaerobic membrane bioreactor (AnMBR) has considerable potential for treating wastewater, although there is very little data on the effect of antibiotics on AnMBR performance. This study examined the effect of Ciprofloxacin (CIP) - an antibiotic that can occur at high concentrations, and has a substantial impact on ecosystems, on AnMBR performance. The long-term (44 days) presence of 0.5 mg/L CIP in the feed did not have a strong effect on COD removal, volatile fatty acid (VFA) accumulation, or methane yield, but did affect the pH, soluble microbial products (SMPs) and suspended solids. However, at 4.7 mg/L CIP, a significant effect on all the parameters tested was seen. 16S rRNA gene-based community analysis demonstrated that CIP changed the phylogenetic structure and altered the species richness and diversity. The relative abundance of various genera was also changed, and this explained much of the change in AnMBR behavior.


Assuntos
Ciprofloxacina , Ecossistema , Anaerobiose , Reatores Biológicos , Membranas Artificiais , Metano , Filogenia , RNA Ribossômico 16S/genética , Esgotos , Eliminação de Resíduos Líquidos , Águas Residuárias
8.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120255, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34464919

RESUMO

For the estimation of some co-administered antimicrobials, two highly accurate and precise spectrofluorimetric methods were developed. Fluconazole (FLZ) is co-administered with either ciprofloxacin (CPR) or ofloxacin (OFX) for the treatment of certain microbial infections. On the other hand, another antimicrobial drug, vancomycin (VNC) is co-administered with ciprofloxacin (CPR) for peritonitis treatment. In method I, conventional spectrofluorimetry has been introduced for the concurrent quantitative estimation of FLZ in presence of OFX or CPR. While in method II, a first derivative synchronous spectrofluorimetric technique was adapted for quantitation of VNC and CPR co-administered combination. Both of them were utilized for estimation of the considered drugs in raw materials, laboratory prepared mixtures, dosage forms, and biological fluids. Method I was relied on simultaneous measuring of the native fluorescence of FLZ and OFX or CPR without any overlapping between the emission spectra of each binary mixture (FLZ / OFX) and (FLZ / CPR). Fluorescence intensities were measured at 283.0, 483.0 and 436.0 nm after excitation at 262.0, 292.0 and 275.0 nm for FLZ, OFX and CPR, respectively. Method II was utilized the synchronous fluorescence intensity of VNC and CPR in methanol at Δλ = 40 nm. The first derivative synchronous spectra were calibrated at 297.0 nm for VNC and at 379.5 nm for CPR. Different variables influencing conventional and synchronous fluorescence intensities of the four antimicrobials under investigation were precisely optimized. Both methods were successfully investigated for the determination of the studied drugs in plasma. The linear data analysis for the calibration curves reveals a good relationship in the ranges of 1.0-10.0, 0.25-2.5 and 0.06-0.6 µg/mL for FLZ, OFX and CPR for method I with limits of detection 0.144, 0.038 and 0.007 µg/mL and limits of quantitation of 0.437, 0.114 and 0.021 µg/mL for FLZ, OFX and CPR, respectively. Linearity range for method II was 0.5 -10.0 µg/mL for VNC and CPR with detection limits of 0.127 and 0.110 µg/mL and quantitation limits of 0.380 and 0.334 µg/mL for VNC and CPR, respectively. International Council on Harmonization ICH Q2 (R1) Guidelines were followed in the developed methods validation. The achieved outcomes were statistically compared with those found by the reported ones, and no significant difference was observed.


Assuntos
Anti-Infecciosos , Preparações Farmacêuticas , Calibragem , Ciprofloxacina , Espectrometria de Fluorescência
9.
Chemosphere ; 287(Pt 1): 131962, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34450369

RESUMO

A fish scale-based porous activated biochar with defined pore size (DPBC) was fabricated by a one-step calcination and activation method. The DPBC possessed an ultrahigh specific surface area of 3370 m2 g-1 and its pore diameter centered at 1.49 nm which fits into the ciprofloxacin (CIP) molecular dimension, making it an ideal adsorbent for CIP adsorption due to the molecular pore-filling effect. The maximum Langmuir monolayer adsorption capacity of DPBC for CIP was higher than 1000 mg g-1 and the equilibrium time was less than 4 h, superior to most adsorbents reported in literature. Thermodynamic analysis indicated the adsorption process was spontaneous and endothermic. Notably, fixed-bed experiments showed an encouraging adsorption performance towards CIP, with a high saturated dynamic adsorption capacity of 880.3 mg g-1. Both Thomas and Yoon-Nelson models predict the fixed-bed column adsorption performance well. Hydrophobic effect, π-π interaction, π-π EDA, cation exchange, hydrogen bonding formation, pore filling effect, electrostatic and cation-π interaction involved in the CIP adsorption on the DPBC.


Assuntos
Ciprofloxacina , Poluentes Químicos da Água , Adsorção , Animais , Carvão Vegetal , Cinética , Poluentes Químicos da Água/análise
10.
Medicine (Baltimore) ; 100(41): e27539, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34731153

RESUMO

ABSTRACT: The present study aimed to compare infectious complications in men undergoing transrectal ultrasound-guided prostate biopsy (TRUS-Bx) with and without povidone-iodine transrectal injection using a gavage syringe.The records of 112 patients, who underwent TRUS-Bx between January 2016 and December 2019, were retrospectively reviewed. The biopsy indication was considered high prostate-specific antigen (PSA) level and/or suspicious digital rectal prostate examination findings. Patients' ages, underlying diseases, PSA levels, prostate volumes, pathologic results, and infectious complications after the biopsy were investigated. All the patients received 1500 mg of ciprofloxacin (750 mg twice a day) for 5 days, starting from the day before the procedure. Forty-seven (41.96%) patients received ciprofloxacin prophylaxis with povidone-iodine transrectal injection, while 65 (58.03%) only received ciprofloxacin prophylaxis. All the patients, who were readmitted to the hospital after the procedure, especially with a temperature of higher than 37.8°C, were detected. For the purposes of the study, the priority was placed on the emergence of the rate of febrile infectious complications. Differences in febrile infectious complications in patients, who received ciprofloxacin prophylaxis with transrectal povidone-iodine, and those, who received ciprofloxacin prophylaxis alone before TRUS-Bx, were studied.Febrile infectious complications developed in 10 cases (15.38%) in patients, who received ciprofloxacin antibiotics prophylaxis alone. In the povidone-iodine rectal disinfection group, there was only 1 case of febrile infectious complication (2%). There was no significant difference by clinicopathologic features, age, PSA level, and cancer detection rate between both groups (P > .05). Multivariate logistic regression analysis did not identify any patient subgroups at a significantly higher risk of infection after prostate biopsy. There was no significant side effect associated with povidone iodine.In addition to the use of prophylactic antibiotics, transrectal povidone-iodine was useful in reducing the febrile infection complications following TRUS-Bx.


Assuntos
Infecções Bacterianas/prevenção & controle , Biópsia Guiada por Imagem/efeitos adversos , Povidona-Iodo/farmacologia , Próstata/patologia , Ultrassonografia de Intervenção/métodos , Administração Retal , Idoso , Antibacterianos/administração & dosagem , Antibacterianos/uso terapêutico , Anti-Infecciosos Locais/administração & dosagem , Anti-Infecciosos Locais/farmacologia , Antibioticoprofilaxia/métodos , Infecções Bacterianas/tratamento farmacológico , Estudos de Casos e Controles , Ciprofloxacina/administração & dosagem , Ciprofloxacina/uso terapêutico , Eficiência , Humanos , Biópsia Guiada por Imagem/tendências , Masculino , Pessoa de Meia-Idade , Ensaios Clínicos Controlados não Aleatórios como Assunto , Povidona-Iodo/administração & dosagem , Antígeno Prostático Específico/sangue , Reto/cirurgia , Estudos Retrospectivos , Fatores de Risco
11.
Wiad Lek ; 74(9 cz 2): 2265-2276, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34824170

RESUMO

OBJECTIVE: The aim of this study is to investigate the role of prodigiosin on P. aeruginosa' s biofilm genes involved in the pathogenicity and persistency of the bacteria. PATIENTS AND METHODS: Materials and methods: Gram negative bacterial isolates were taken from burn and wounds specimen obtained from some of Baghdad hospitals. Forty six isolates were identified as Pseudomonas aeruginosa and four isolates as Serratia marcescens by using biochemical tests and VITEK 2 compact system. Susceptibility test was performed for all P. aeruginosa isolates, the results showed that 100% were resistant to Amikacin and 98% were sensitive to Meropenem. Resistant isolates were tested for biofilm formation; the strong and moderate isolates (17) were detected by PCR for AlgD gene presence. From 17 isolates only two had AlgD gene. All serratia isolates were screened for prodigiosin production, which were extracted from the best producer isolate. Minimal inhibitory concentration was assessed for prodigiosin and ciprofloxacin and synergism between them against the two isolates of P. aeruginosa. RESULTS: Results and conclusions: The results showed that the synergistic effect decreased MIC of both prodigiosin and ciprofloxacin by combination, and reduction of biofilm formation was detected. RNA was extracted from the two selected isolates as control in addition to three treatments. The result of quantitative real time PCR showed down regulation in the AlgD gene expression level under some treatments, while there was no gene expression in most treatments with both sub-MICs treatment.


Assuntos
Prodigiosina , Pseudomonas aeruginosa , Antibacterianos/farmacologia , Ciprofloxacina , Humanos , Testes de Sensibilidade Microbiana , Prodigiosina/farmacologia , Pseudomonas aeruginosa/genética
12.
Ann Palliat Med ; 10(9): 9765-9771, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34628902

RESUMO

BACKGROUND: The efficacy of levofloxacin and ciprofloxacin in the treatment of urinary tract infection is not clear yet. This study perform a meta-analysis to explore the differences between the two against urinary tract infection (UTI). METHODS: A computerized literature search was conducted of the databases of PubMed, Medline, Embase, and the Cochrane Library. All the retrieved literatures were randomized comparative studies of levofloxacin and ciprofloxacin. The included studies were screened according to the standard of nanofiltration. The risk of bias was assessed with RevMan 5.3.5 software. The treatment effect index and incidence of adverse reactions index were established and compared via meta-analysis. RESULTS: A total of 5 studies were included, involving 2,877 patients overall. The results showed that levofloxacin was more effective than ciprofloxacin, but the difference between the 2 drugs was not statistically significant [odds ratio (OR) =1.18, 95% confidence interval (CI): 0.94 to 1.46, P=0.15]. There was also no statistical significance in the rate of adverse reactions between the 2 drugs (OR =0.91, 95% CI: 0.78 to 1.07, P=0.27). DISCUSSION: In the treatment of UTI, the efficacy and safety of levofloxacin and ciprofloxacin are similar statistically. If bacterial resistance is discovered after the treatment of one of the drugs, the other drug might become an alternative.


Assuntos
Levofloxacino , Infecções Urinárias , Ciprofloxacina/uso terapêutico , Humanos , Levofloxacino/uso terapêutico , Infecções Urinárias/tratamento farmacológico
13.
J Wound Care ; 30(Sup9a): IVi-IVx, 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34597167

RESUMO

AIM: Self-healing, swellable and biodegradable polymers are vital materials that may facilitate the different stages of wound healing. The aim of this research was to prepare wound healing films using self-healing polyvinyl alcohol (PVA), swellable hydroxypropyl methylcellulose (HPMC), biodegradable polyglycolic acid (PGA) sutures and ciprofloxacin antibiotic for improved treatment outcome. METHODS: Films were formulated through aqueous-based mixing of varying amounts of polyvinyl alcohol (10-20% weight/weight (w/w)) and hydroxypropyl methylcellulose (0.5, 1% w/w) with fixed quantities of ciprofloxacin. PGA sutures were placed as grids within the wet mixtures of the polymers and ciprofloxacin, and thereafter products were air dried. The formulated films were evaluated for swelling ratio, breaking elongation, folding endurance, moisture uptake and loss, compatibility and in vitro antibiotic release. Furthermore, in vivo wound healing was studied using excision model and histopathological examinations. RESULTS: Swelling ratios were above 1.0 and the films were minimally stretchable, with folding endurance greater than 500. Films were stable while moisture uptake and loss were observed to be less than 30%. Among the optimised hydrogel batches, those containing 10% w/w PVA and 1% w/w HPMC with no PGA showed the highest drug release of 73%, whereas the batches with higher PGA content showed higher percentage wound size reduction with minimal scar. The completeness of wound healing with batches containing PVA, HPMC, ciprofloxacin and PGA, along with the standard, is evident considering the massive cornification, regeneration of the epithelial front and stratum spinosum. CONCLUSION: The findings show that polymer-based multifunctional composite films are suitable for use as dressings for improved wound healing.


Assuntos
Ciprofloxacina , Polímeros , Bandagens , Hidrogéis , Cicatrização
14.
Int J Mol Sci ; 22(19)2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34638966

RESUMO

Bacterial non-coding RNAs (ncRNAs) play important regulatory roles in various physiological metabolic pathways. In this study, a novel ncRNA CsiR (ciprofloxacin stress-induced ncRNA) involved in the regulation of ciprofloxacin resistance in the foodborne multidrug-resistant Proteus vulgaris (P. vulgaris) strain P3M was identified. The survival rate of the CsiR-deficient strain was higher than that of the wild-type strain P3M under the ciprofloxacin treatment condition, indicating that CsiR played a negative regulatory role, and its target gene emrB was identified through further target prediction, quantitative real-time PCR (qRT-PCR), and microscale thermophoresis (MST). Further studies showed that the interaction between CsiR and emrB mRNA affected the stability of the latter at the post-transcriptional level to a large degree, and ultimately affected the ciprofloxacin resistance of P3M. Notably, the base-pairing sites between CsiR and emrB mRNAs were highly conserved in other sequenced P. vulgaris strains, suggesting that this regulatory mechanism may be ubiquitous in this species. To the best of our knowledge, this is the first identification of a novel ncRNA involved in the regulation of ciprofloxacin resistance in P. vulgaris species, which lays a solid foundation for comprehensively expounding the antibiotic resistance mechanism of P. vulgaris.


Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/metabolismo , Ciprofloxacina/farmacologia , Farmacorresistência Bacteriana/genética , Proteínas de Membrana/metabolismo , Proteus vulgaris/efeitos dos fármacos , Proteus vulgaris/metabolismo , RNA não Traduzido/metabolismo , Proteínas de Bactérias/genética , Farmacorresistência Bacteriana/efeitos dos fármacos , Deleção de Genes , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Proteínas de Membrana/genética , Organismos Geneticamente Modificados , Proteus vulgaris/genética , RNA Mensageiro/metabolismo , RNA não Traduzido/genética , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética
15.
J Trop Pediatr ; 67(4)2021 08 27.
Artigo em Inglês | MEDLINE | ID: mdl-34471923

RESUMO

Chromobacterium violaceum, a facultative anaerobic proteobacterium, is isolated from water and soil in tropical areas and has been implicated in infections like septicemia, visceral abscesses, skin and soft tissue infections, meningitis and diarrhea. Chromobacterium violaceum sepsis, a rarely reported phenomenon has a very high mortality rate. Here, we report a unique case of Chromobacterium sepsis in an infant. A 48-day-old baby boy was referred to our institution with h/o fever, loose stools and reduced activity. He was intubated and referred to us in septic shock. Radiological investigations revealed multiple abscesses in the liver, spleen and kidneys. The infant was successfully treated with trimethoprim-sulfamethoxazole and ciprofloxacin.


Assuntos
Infecções por Bactérias Gram-Negativas , Sepse , Antibacterianos/uso terapêutico , Chromobacterium , Ciprofloxacina/uso terapêutico , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Humanos , Lactente , Masculino , Sepse/diagnóstico , Sepse/tratamento farmacológico
16.
J Hazard Mater ; 416: 125988, 2021 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492885

RESUMO

It is important to develop highly-active photoelectrochemical (PEC) materials and use novel sensing strategy for constructing high-PEC-performance sensors with multiplex detection abilities, owing to the simultaneous presence of multiple antibiotic residues in food. Herein, a bias-potential-based PEC aptasensor was prepared for the trace detection of dual antibiotic analytes, enrofloxacin (ENR) and ciprofloxacin (CIP), which often coexist in milk samples. Here, two materials were developed with excellent PEC performance: three-dimensional nitrogen-doped graphene-loaded copper indium disulfide (CuInS2/3DNG) and Bi3+-doped black anatase titania nanoparticles decorated with reduced graphene oxide (Bi3+/B-TiO2/rGO). By applying different bias potentials to the two materials near one ITO electrode, the cathodic current generated by CuInS2/3DNH and the anodic current generated by Bi3+/B-TiO2/rGO could be clearly distinguished without interfering with each other. Then, ENR and CIP aptamers were respectively modified onto the surface of CuInS2/3DNH and Bi3+/B-TiO2/rGO to construct a PEC aptasensor for the sensitive detection of ENR and CIP. Under optimal conditions, the proposed aptasensor exhibited wide linear ranges of ENR (0.01-10000 ng/mL) and CIP (0.01-1000 ng/mL), and relatively low detection limits of 3.3 pg/mL to ENR and CIP (S/N = 3). The aptasensor was successfully applied to the detection of ENR and CIP in milk samples.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Grafite , Animais , Ciprofloxacina , Técnicas Eletroquímicas , Enrofloxacina , Limite de Detecção , Leite
17.
Mater Sci Eng C Mater Biol Appl ; 128: 112292, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34474843

RESUMO

The ever-growing threat of drug-resistant pathogens and their biofilms based persistent, chronic infections has created an urgent call for new strategies to deal with multidrug resistant bacteria (MDR). Near-infrared (NIR) laser-induced photothermal treatment (PTT) of gold nanorods (AuNRs) disinfects microbes by local heating with low possibility to develop resistant. However, PTT disinfection strategy of AuNRs alone shows less efficiency in killing multidrug resistant strains (i.e. Methicillin-resistant Staphylococcus aureus, MRSA) and their matured biofilms. Herein, a novel synergistic chemo-photothermal integrated antimicrobial platform (P(Cip-b-CB)-AuNRs) was fabricated which show enhanced killing efficiency against MRSA in both planktonic and biofilm phenotypes. Polymethacrylate copolymers with pendant ciprofloxacin (Cip) and the carboxyl betaine groups (P(Cip-b-CB)) were synthesized using reversible addition-fragmentation chain transfer (RAFT) polymerization. P(Cip-b-CB) was decorated onto AuNRs via gold-thiol bond which resulted in AuNRs with acidic-induced surface charge-switchable activities and lipase triggered Cip release properties (P(Cip-b-CB)-AuNRs). The lower pH value and overexpress of lipase are characteristics for microenvironment of microbial infections and their biofilms, which ensure the targeting on, penetration into and on-demand release of Cip from the nanocomposites in bacterial infection sites and their biofilms. The bacterial cell membrane was disrupt by photothermal therapy which could improve its permeability and sensitivity to antibiotics, meanwhile lipase-triggered release of Cip ensures a high concentration of antibiotics at the site of bacterial infection. Besides their NIR induced PTT disinfection activities, the increased local temperature generated by NIR light irradiation accelerated Cip release which further enhanced the antibacterial efficiency, leading to synergistic antibacterial activities of chemo-photothermal therapy. Taken together, the designed synergistic chemo-photothermal integrated antimicrobial platform is a promising antibacterial agent for fighting MDR bacterial infections and their biofilms.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Nanotubos , Preparações Farmacêuticas , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Biofilmes , Ciprofloxacina/farmacologia , Ouro , Concentração de Íons de Hidrogênio
18.
Molecules ; 26(17)2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34500595

RESUMO

Amine-containing drugs often show poor pharmacological properties, but these disadvantages can be overcome by using a prodrug approach involving self-immolative linkers. Accordingly, we designed l-lactate linkers as ideal candidates for amine delivery. Furthermore, we designed linkers bearing two different cargos (aniline and phenol) for preferential amine cargo release within 15 min. Since the linkers carrying secondary amine cargo showed high stability at physiological pH, we used our strategy to prepare phosphate-based prodrugs of the antibiotic Ciprofloxacin. Therefore, our study will facilitate the rational design of new and more effective drug delivery systems for amine-containing drugs.


Assuntos
Aminas/química , Preparações Farmacêuticas/química , Fosfatos/química , Pró-Fármacos/química , Antibacterianos/química , Ciprofloxacina/química , Sistemas de Liberação de Medicamentos/métodos , Concentração de Íons de Hidrogênio , Ácido Láctico/química
19.
Molecules ; 26(18)2021 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-34577155

RESUMO

An innovative, rapid and stable method for simultaneous determination of three tetracycline (oxytetracycline, tetracycline and doxycycline) and two fluoroquinolone (ciprofloxacin and enrofloxacin) residues in poultry eggs by ultra-high performance liquid chromatography-fluorescence detection (UPLC-FLD) was established and optimized. The samples were homogenized and extracted with acetonitrile/ultrapure water (90:10, v/v) and then purified by solid-phase extraction (SPE). LC separation was achieved on an ACQUITY UPLC BEH C18 column (1.7 µm, 2.1 mm × 100 mm), and the mobile phase was composed of acetonitrile and a 0.1 mol/L malonic acid solution containing 50 mmol/L magnesium chloride (the pH was adjusted to 5.5 with ammonia). When the five target drugs were spiked at the limit of quantification, 0.5 times the maximum residue limit (MRL), 1.0 MRL and 2.0 MRL, the recoveries were above 83.5% and the precision ranged from 1.99% to 6.24%. These figures of merit complied with the parameter validation regulations of the EU and U.S. FDA. The limits of detection and quantifications of the targets were 0.1-13.4 µg/kg and 0.3-40.1 µg/kg, respectively. The proposed method was easily extended to quantitative analyses of target drug residues in 85 egg samples, thus demonstrating its reliability and applicability.


Assuntos
Antibacterianos/análise , Cromatografia Líquida de Alta Pressão/métodos , Ovos/análise , Fluoroquinolonas/análise , Espectrometria de Fluorescência/métodos , Tetraciclinas/análise , Animais , Ciprofloxacina/análise , Doxiciclina/análise , Resíduos de Drogas/análise , Enrofloxacina/análise , Contaminação de Alimentos/análise , Limite de Detecção , Oxitetraciclina/análise , Aves Domésticas , Reprodutibilidade dos Testes , Extração em Fase Sólida , Tetraciclina/análise , Drogas Veterinárias/análise
20.
Environ Pollut ; 291: 118095, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34537598

RESUMO

Ciprofloxacin (CFX) and ofloxacin (OFX) are two of the most often used fluoroquinolone antibiotics, and their residues are found in large amounts in various aquatic settings. However, the toxicity tests of CFX using eukaryotic organisms such as Daphnia magna are inadequate, and the test result of OFX is currently unknown. Therefore, the chronic toxicity test for D. magna was performed during 42 days under exposure to CFX and OFX concentrations of 50, 500, and 5000 µg L-1. All exposure conditions did not cause mortality for D. magna. CFX exposure at 500 µg L-1 resulted in an earlier oogenesis date and increased brood size in the second birth. The Poisson-based generalized linear mixed-effects model revealed that the reduction of fertility was statistically significant for the CFX and OFX exposures at 5000 µg L-1. On the other hand, the production of dead eggs as offspring degradation was also found significantly as maternal D. magna exposed to antibiotics at 5000 µg L-1. In addition, following long-term exposure to antibiotics, maternal adaptation to antibiotics was established for offspring deterioration and fertility. However, the OFX exposure showed that the fertility-suppressed effects continued for a longer period than the CFX exposure. Although no rational explanation has yet been given for the more substantial effect of OFX on reducing fertility than CFX, molecular cell biology and symbiotic microbial flora derived from previous studies could explain our ecotoxicological results. This study is the first report for the OFX chronic toxicities on D. magna by comparing it to the toxicity of CFX. Our study contributes to guiding the future impact assessment of fluoroquinolone antibiotic pollution on ecosystems, including the need for new statistical methods in ecotoxicological studies.


Assuntos
Daphnia , Poluentes Químicos da Água , Animais , Ciprofloxacina/toxicidade , Ecossistema , Ecotoxicologia , Ofloxacino/toxicidade , Reprodução , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade
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