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1.
Int J Nanomedicine ; 14: 7781-7792, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31576122

RESUMO

Purpose: Mesoporous silica (MS) have been considered as a biocompatible compound and found to have various pharmaceutical applications. Recently, novel approaches in applications of MS as antidote agents were introduced. In this study, the capacity of ethylenediaminetetraacetic acid modified mesoporous silica (MS-EDTA) was evaluated in in vitro and in vivo adsorption of copper (Cu). Methods: The MS-EDTA was characterized by fourier transform infrared (FT-IR) and X-ray diffraction, while surface area was determined by N2 adsorption-desorption technique. Morphological studies were observed by high resolution-transmission electron microscopy and field emission-scanning electron microscopy and the sizes were determined by dynamic light scattering. The capacity of these particles for copper adsorption was investigated in vitro in both 1.2 and 7.2 pH. In in vivo animal study, the Cu adsorption efficiency of MS-EDTA in Cu-overdosed mice was evaluated. In this case, an animal model of acute copper poisoning was prepared. Results: The MS-EDTA with surface area of 352.35 was synthesized. Scanning electron microscope showed spherical particle formation with less than 500 nm in size. Transmission electron microscope images showed porous and honeycomb structure. FT-IR spectroscopy showed an appropriate formation of functional groups. Particle efficiency was investigated for Cu adsorption. MS-EDTA in both media showed a high adsorption capability for Cu (II) adsorption in pH=1.2 and pH=7.2. In addition, the study of Langmuir, Freundlich, and Redlich-Peterson adsorption models showed that copper adsorption by MS-EDTA followed the Freundlich model with multi-layer adsorption. In vivo evaluation showed that MS-EDTA could alleviate the symptoms of acute copper poisoning by lowering Cu plasma levels. Conclusion: Structural evaluation showed successful formation of MS-EDTA. In vitro analysis demonstrated that supreme Cu adsorption occurs in both pH conditions (7.2 and 1.2), and was especially more favorable in simulated intestinal pH (7.2). The in vivo studies in animal models with acute Cu poisoning showed that MS-EDTA could be a potent antidote agent.


Assuntos
Antídotos/farmacologia , Cobre/isolamento & purificação , Cobre/toxicidade , Ácido Edético/química , Dióxido de Silício/química , Adsorção , Animais , Antídotos/administração & dosagem , Biomarcadores/sangue , Cobre/sangue , Difusão , Íons , Cinética , Camundongos , Modelos Animais , Tamanho da Partícula , Porosidade , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
3.
Nutrients ; 11(8)2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31366053

RESUMO

BACKGROUND: Obesity-induced inflammation is frequently associated with higher oxidative stress. In vitro and experimental studies have considered baru almonds (Dipteryx alata Vog) as a legume seed with high antioxidant capacity. The aim of this study was to evaluate whether baru almonds are capable of improving the inflammatory and antioxidant status in overweight and obese women. METHODS: In a parallel-arm, randomized placebo-controlled trial, 46 overweight and obese women (age: 40 ± 11 years; body mass index: 33.3 ± 4.3) were randomly assigned to receive advice to follow a normocaloric and isoenergetic diet with placebo (PLA, n = 22) or similar advice plus 20 g baru almonds (BARU, n = 24) for 8 wk. Malondialdehyde (MDA), adiponectin, tumor necrosis factor-α, interleukin-6, interleukin-10, antioxidant enzymes activities (catalase-CAT; glutathione peroxidase-GPx; superoxide dismutase-SOD), and minerals were analyzed in plasma samples. RESULTS: At baseline, groups were similar regarding the body composition, oxidative, and inflammatory parameters. The BARU group increased the activity of GPx (+0.08 U/mg, 95%CI + 0.05 to +0.12 vs. -0.07, 95%CI -0.12 to -0.03, p < 0.01) and plasma copper concentration (p = 0.037) when compared to the PLA group. No differences were observed between groups in CAT and SOD activity or MDA and cytokines concentrations. CONCLUSIONS: Baru almond supplementation increased the GPx activity in overweight and obese women.


Assuntos
Glutationa Peroxidase/metabolismo , Sobrepeso/dietoterapia , Prunus dulcis , Adulto , Composição Corporal , Cobre/sangue , Feminino , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Glutationa Peroxidase/genética , Humanos
4.
Environ Monit Assess ; 191(9): 537, 2019 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-31377885

RESUMO

Copper contamination is increasing and can be a threat to human health. This review tries to summarize copper levels measured in humans in Iran. Persian databases such as SID, Magiran, and IranMedex and English databases such as Scopus, Pubmed, Science Direct, and the Google Scholar were searched using both English and Persian keywords. Twenty-six articles that measured the concentration of copper in human samples in Iran were included. According to the results of the reviewed studies, copper levels in some Iranian populations were higher than normal levels. These populations included pregnant women with preeclampsia, patients with oral cancer, patients with Giardiasis infection, patients with Parkinson's, children under the age of 12 years with ß-thalassemia major, pregnant women in the third trimester, and type 2 diabetic patients. Copper levels were less than normal, in patients with tuberculosis after treatment and post-menopausal women with osteopenia and osteoporosis. Also, copper concentrations in patients with tuberculosis, cutaneous leishmaniasis, brucellosis, and molybdenum unit workers were higher, and copper concentrations in patients with Pemphigus vulgaris and coronary artery disease were less than those of their controls, but all were in the normal range (70-140 µg/dL). The amount of copper adsorption in various teeth was different. High levels of copper have been reported in some Iranian populations and this can be a threat to human health. Monitoring copper levels in some Iranian populations is necessary.


Assuntos
Cobre/sangue , Criança , Bases de Dados Factuais , Exposição Ambiental , Feminino , Humanos , Irã (Geográfico) , Exposição Ocupacional , Pacientes , População , Gravidez
5.
Acta Neurobiol Exp (Wars) ; 79(2): 184-192, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31342954

RESUMO

Depression is a chronic illness of unknown etiology. Trace elements, such as copper and zinc, and defense antioxidants, such as catalase, are important factors that determine the clinical course of brain diseases. Furthermore, altered glucose metabolism in hippocampus and prefrontal cortex has been associated with depression. Identifying factors that can precipitate depressive-like behavior is of particular importance as it can direct clinicians towards the etiology of the disease. In this study, 16 male Sprague-Dawley rats were randomly divided into two groups: socialized and socially isolated. After one week of acclimatization, animals were housed in isolation for 14 days. Rats in the social group were socialized together for 14 days. On day 15, the forced swim test was performed and blood sugar was analyzed. The brain was removed immediately for biochemical analysis. Socially isolated rats showed more pronounced depressive-like behavior in the forced swim test than socialized rats. Moreover, socially isolated rats demonstrated significantly lower copper and zinc concentrations, as well as a marked reduction in catalase activity, in both prefrontal cortex and hippocampus compared to socialized rats. Additionally, blood sugar levels were higher in socially isolated animals. Isolation causes reduction in copper and zinc levels and catalase activity, which may precipitate depressive-like behavior in these animals.


Assuntos
Catalase/metabolismo , Cobre/sangue , Hipocampo/metabolismo , Estresse Psicológico/fisiopatologia , Zinco/sangue , Animais , Antioxidantes/metabolismo , Depressão/metabolismo , Transtorno Depressivo/fisiopatologia , Hipocampo/fisiopatologia , Masculino , Córtex Pré-Frontal/metabolismo , Córtex Pré-Frontal/fisiopatologia , Ratos Sprague-Dawley , Isolamento Social
6.
Nutrients ; 11(7)2019 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-31315178

RESUMO

Trace elements such as zinc, copper, and selenium are essential for reproductive health, but there is limited work examining how circulating trace elements may associate with fertility in humans. The aim of this study was to determine the association between maternal plasma concentrations of zinc, copper, and selenium, and time to pregnancy and subfertility. Australian women (n = 1060) who participated in the multi-centre prospective Screening for Pregnancy Endpoints study were included. Maternal plasma concentrations of copper, zinc and selenium were assessed at 15 ± 1 weeks' gestation. Estimates of retrospectively reported time to pregnancy were documented as number of months to conceive; subfertility was defined as taking more than 12 months to conceive. A range of maternal and paternal adjustments were included. Women who had lower zinc (time ratio, 1.20 (0.99-1.44)) or who had lower selenium concentrations (1.19 (1.01-1.40)) had a longer time to pregnancy, equivalent to a median difference in time to pregnancy of around 0.6 months. Women with low selenium concentrations were also at a 1.46 (1.06-2.03) greater relative risk for subfertility compared to women with higher selenium concentrations. There were no associations between copper and time to pregnancy or subfertility. Lower selenium and zinc trace element concentrations, which likely reflect lower dietary intakes, associate with a longer time to pregnancy. Further research supporting our work is required, which may inform recommendations to increase maternal trace element intake in women planning a pregnancy.


Assuntos
Cobre/sangue , Infertilidade Feminina/sangue , Estado Nutricional , Fenômenos Fisiológicos da Nutrição Pré-Natal , Selênio/sangue , Zinco/sangue , Adolescente , Adulto , Feminino , Humanos , Gravidez , Fatores de Risco , Adulto Jovem
7.
Anal Bioanal Chem ; 411(21): 5593-5603, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31289896

RESUMO

A novel SBA-15-based fluorescent sensor, SBA-PI: mesoporous SBA-15 structure modified with iminostilbene groups, was designed, synthesized, and characterized by Fourier transform-infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), thermogravimetric analysis (TGA), low-angle X-ray diffraction techniques (low-angle XRD), and N2 adsorption-desorption techniques. The SBA-PI as a sensor with a selective behavior for detection of Cu2+ comprises iminostilbene carbonyl as the fluorophore group. The SBA-PI sensor displays an excellent fluorescence response in aqueous solutions and the fluorescence intensity quenches remarkably upon addition of Cu2+. Other common interfering ions even at high concentration ratio showed either no or very small changes in the fluorescence intensity of SBA-PI in the absence of Cu2+. A limit of detection of 8.7 × 10-9 M for Cu2+ indicated that this fluorescence sensor has a high sensitivity and selectivity toward the target copper (II) ion. The fabricated Cu2+ sensor was successfully applied for the determination of the Cu2+ in human blood samples without any significant interference. With the selective analysis of Cu2+ ions down to 0.9 nM in blood, the sensor is a promising and a novel detection candidate for Cu2+ and can be applied in the clinical laboratory. A reversibility and accuracy in the fluorescence behavior of the sensor was found in the presence of I¯ that was described as a masking agent for Cu2+. Graphical abstract.


Assuntos
Cobre/sangue , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Oligoelementos/sangue , Humanos , Concentração de Íons de Hidrogênio , Limite de Detecção , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Difração de Pó , Espectrometria por Raios X , Termogravimetria
8.
Zoo Biol ; 38(4): 371-383, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31257640

RESUMO

The longevity of zoo animals is increasing due to continuous improvement in husbandry and veterinary medicine. However, increasing age is correlated to a higher prevalence of neoplasia. Despite tremendous improvement in diagnoses and monitoring capacities, cancers are still a challenge for veterinarians within the global zoo community. The recent use of copper isotopes as biomarkers for neoplasia in both human and veterinary medicine is a promising and cost-effective diagnostic tool. Two hundred and twenty-nine serum samples from 10 different species of wild felids under human care were processed through mass spectrometry to determine the ratio of heavy and light copper isotopes (65 Cu/63 Cu). The results of this preliminary study exhibit an important variability between felid species, with a ratio ranging between -1.71 and 0.63. Additionally, copper isotopes seem to be a promising diagnostic tool in monitoring cancer in wild animals, as in human medicine, where the isotopic ratio decreases significantly with time in the presence of a tumor.


Assuntos
Cobre/sangue , Felidae/sangue , Neoplasias/veterinária , Animais , Biomarcadores Tumorais , Feminino , Masculino , Neoplasias/sangue , Neoplasias/diagnóstico , Especificidade da Espécie
9.
Talanta ; 204: 663-669, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31357350

RESUMO

A high-throughput, sensitive and rapid method was developed for the determination of Zn, Cu and Fe in small volumes (30 µL) of human serum using inductively coupled plasma mass spectrometry (ICP-MS). The sample preparation procedure employed simple 100-fold dilution of the serum samples with 1.0% butanol, 0.5% v/v ammonia, 0.02% v/v Triton X-100 and 0.01% v/v HNO3. The reliability of the method was evaluated using serum UTAK certified reference material, and the results matched well with the certified values. The method was applied to determine Zn, Cu and Fe in 81 human serum samples from participants in Alzheimer disease (AD) and age-related macular degeneration (AMD) studies. No significant differences were found in Zn and Cu levels between age matched controls, AD and AMD patients. Whilst iron levels appeared marginally higher in the AMD group, compared with the AD group, iron showed larger overall variability than the other two elements.


Assuntos
Cobre/sangue , Ferro/sangue , Espectrometria de Massas/métodos , Zinco/sangue , Doença de Alzheimer/sangue , Amônia/química , Humanos , Degeneração Macular/sangue , Ácido Nítrico/química , Octoxinol/química
11.
Clin Lab ; 65(6)2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31232016

RESUMO

BACKGROUND: Zinc and copper are among the most important trace elements. Deficiencies of these trace elements cause a wide variety of disorders. The present study aims to report the definitive assessment of biological variation (BV) parameters for these elements as within-subject BV (CVI), between subject BV (CVG), index of individuality (II), and reference change value (RCV) in a Turkish cohort study group. METHODS: Ten blood specimens were collected weekly from 20 healthy volunteers (13 women, 7 men) for 10 weeks. Collected sera were stored at -80°C until the time of analysis. Serum zinc and copper levels were analyzed with atomic absorption spectrometry and ANOVA test was used to calculate the variations. RESULTS: The CVI and CVG for zinc were 6.26% and 23.27%, respectively. Analytical variation (CVA) was calculated as 4.24%. II and RCV for zinc were calculated as 0.26 and 21.51%, respectively. The CVI and CVG for copper were 6.05% and 19.64%, respectively. CVA was calculated as 4.24%. II and RCV for copper were calculated as 0.31 and 20.47%, respectively. CONCLUSIONS: Since II values were less than 0.6 for both analytes, the reference values will be of little use. RCV might be preferred for better evaluation instead.


Assuntos
Coleta de Amostras Sanguíneas/métodos , Voluntários Saudáveis , Zinco/sangue , Adulto , Estudos de Coortes , Cobre/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Espectrofotometria Atômica
12.
Gac Med Mex ; 155(2): 191-195, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31056589

RESUMO

Menkes disease is a neurodegenerative and lethal pathology caused by gene mutations of the copper-transporting ATP-7A enzyme; it manifests itself by neurological symptoms and connective tissue changes of varying severity. Timely subcutaneous use of copper histidinate (Cu-His) is determinant for quality of life. We report the first experiences in Mexico on Cu-His synthesis and its safe use in 3 cases where hypocupremia and hypoceruloplasminemia were corroborated. With advice of the Hospital for Sick Children of Toronto Canada, we prepared a 500 µg/mL solution. In all three cases were 250 µg of Cu-His applied without relevant undesirable effects for 30 days. Serum copper (Cu, expressed in µg/L) and ceruloplasmin (Cp, in mg/dL) were determined: case 1, Cu days 0 and 30, 8 and 504 µg/L; Cp days 0 and 30, 4 and 10.75 mg/dL; case 2, Cu days 0 and 30, <50 and 502 µg/L; Cp days 0 and 30, 2 and 15 mg/dL; case 3, Cu days 0 and 30, 3 and 84.2 µg/L; Cp days 0 and 30, 4 and 10.7 mg/dL. In Mexico, it is possible to safely synthesize Cu-His and treat MD, which must be intentionally sought.


Assuntos
Composição de Medicamentos/métodos , Histidina/análogos & derivados , Síndrome dos Cabelos Torcidos/tratamento farmacológico , Compostos Organometálicos/administração & dosagem , Qualidade de Vida , Pré-Escolar , Cobre/sangue , Histidina/administração & dosagem , Histidina/efeitos adversos , Humanos , Lactente , México , Compostos Organometálicos/efeitos adversos , Soluções Farmacêuticas
13.
Theriogenology ; 133: 97-103, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31078069

RESUMO

The role of oxidative stress in the control of female reproduction has not been fully elucidated in ruminants; however, it seems that antioxidants can make influence to the reproductive axis at different levels. Therefore, the aim of this study was to determine the relationship between antioxidant status and concentrations of trace minerals (chromium (Cr), copper (Cu), iron (Fe), and zinc (Zn)) with postpartum luteal activity and fertility in Holstein dairy cows. The cows (n = 100, a parity range of 2-5, and a body condition score (BCS) of 3.0 ±â€¯0.25, mean ±â€¯SEM) were assigned to the experiment at second week post calving. Ovarian follicular dynamics were monitored twice a week (from 3rd to 6th weeks post calving) by transrectal ultrasonography (US). Blood samples were collected twice weekly from the 3rd to the 6th weeks post calving at timed artificial insemination (TAI), and days 32 and 50 post AI to determine superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), and trace mineral concentrations. There were associations between plasma concentrations of SOD, GPX, and TAC with postpartum luteal activity (PLA, P = 0.01) and ovulation (P = 0.03). Mean plasma SOD and GPX activities and TAC levels (U/mL) were greater in cows with normal luteal activity (NLA) than prolonged luteal phase (PLP) and anovulation (AO) cows, as well as in ovulated compared to AO cows (P = 0.03). Pregnant cows had greater levels of SOD, GPX, and TAC (U/mL) at TAI than non-pregnant cows (P = 0.01). Plasma Cu and Zn concentrations increased in pregnant compared to non-pregnant cows at TAI. In conclusion, antioxidant levels and Cu and Zn concentrations were associated with PLA and fertility.


Assuntos
Aborto Animal , Antioxidantes/metabolismo , Corpo Lúteo/fisiologia , Oligoelementos/sangue , Animais , Bovinos , Cromo/sangue , Cobre/sangue , Corpo Lúteo/metabolismo , Feminino , Glutationa Peroxidase/sangue , Inseminação Artificial/veterinária , Ferro/sangue , Período Pós-Parto/metabolismo , Gravidez , Superóxido Dismutase/sangue , Zinco/sangue
14.
Hum Genet ; 138(5): 541-546, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31062085

RESUMO

This preliminary study summarizes the genotypes of 42 Labrador Retrievers and Labrador Retriever-Golden Retriever crosses and phenotypes a subset of ten of these dogs that are homozygous mutant, heterozygous, or homozygous normal for mutations in the ATP7A and ATP7B genes that have been associated with the development of copper toxicosis in Labrador Retrievers. The purpose of this study is to evaluate whether there is a correlation between ATP7A and ATP7B genotypes and clinical evidence of hepatic pathology in young, asymptomatic Labrador Retrievers. We evaluated serum ALT levels, hepatic copper concentrations, and hepatic histopathology from ten offspring where both parents had a least one copy of the ATP7B mutation. Five were homozygous mutant, four were heterozygous, and one was homozygous normal for comparison. None had increased serum ALT activity. All dogs homozygous for the ATP7B mutation had elevated hepatic copper concentrations compared to dogs heterozygous for the ATP7B mutation regardless of sex or presence of an ATP7A mutation with the mean hepatic copper concentration being 1464 ppm (reference range 100-330 ppm). Mean hepatic copper concentration in homozygous normal and heterozygous dogs was 328 ppm. In this preliminary analysis, we found that dogs that carry two copies of the ATP7B mutation have abnormally elevated hepatic copper levels despite having normal serum ALT activity. Our findings support the hypothesis that the ATP7B DNA test can predict defects in hepatic copper metabolism. Veterinarians can test for the ATP7B gene mutation to identify Labrador Retrievers at risk for copper toxicosis so that they can take steps to prevent development of copper-associated chronic hepatitis in their patients.


Assuntos
ATPases Transportadoras de Cobre/genética , Cobre/sangue , Cobre/toxicidade , Doenças do Cão/genética , Cirrose Hepática/diagnóstico , Cirrose Hepática/veterinária , Erros Inatos do Metabolismo dos Metais/diagnóstico , Erros Inatos do Metabolismo dos Metais/veterinária , Alanina Transaminase/sangue , Animais , Cães , Feminino , Predisposição Genética para Doença/genética , Genótipo , Degeneração Hepatolenticular/complicações , Degeneração Hepatolenticular/genética , Humanos , Masculino , Rodanina/metabolismo
15.
Medicina (Kaunas) ; 55(5)2019 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-31117252

RESUMO

Background and Objectives: Altered copper and zinc homeostasis may influence the antioxidant defense system and consequently lead to oxidative stress and associated complications in sickle cell disease (SCD) patients. Iron levels have been reported to increase in sickle cell patients due to frequent blood transfusion, chronic intravenous haemolysis and increased absorption of iron from the gastrointestinal tract. These elevated levels of iron may also lead to extensive oxidative damage. The current study evaluated serum levels of iron, copper and zinc in SCD patients and "healthy" controls. Materials and Methods: The study was a cross-sectional one, comprising 90 SCD patients with Haemoglobin SS and Haemoglobin SC genotypes and 50 HbAA "healthy" controls. Serum levels of iron, copper and zinc were measured using a Flame Atomic Absorption Spectrometer (Variant 240FS manufactured by VARIAN Australia Pty Ltd, VIC, Australia). Copper and zinc ratios were calculated and analyzed. Results: Serum levels of iron and copper were significantly elevated in the SCD patients, compared to their "healthy" counterparts (p < 0.001). These levels were further increased in patients with haemoglobin SS in vaso-occlusive crises (HbSS VOCs). Serum zinc levels were, however, significantly lower in the SCD patients, particularly during vaso-occlusion. The copper-to-zinc ratio was also found to be significantly higher in the SCD patients. Conclusion: Elevated copper-to-zinc ratio may be a biomarker of sickle cell oxidative stress and associated complications. The ratio may also be informative for the management of sickle cell oxidative burden. The significantly lower levels of zinc in the SCD patients may warrant zinc supplementation.


Assuntos
Anemia Falciforme/sangue , Cobre/análise , Ferro/análise , Zinco/análise , Adulto , Anemia Falciforme/complicações , Biomarcadores/análise , Biomarcadores/sangue , Cobre/sangue , Estudos Transversais , Feminino , Gana , Humanos , Ferro/sangue , Masculino , Pessoa de Meia-Idade , Estresse Oxidativo/fisiologia , Zinco/sangue
16.
Wei Sheng Yan Jiu ; 48(2): 238-243, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-31133101

RESUMO

OBJECTIVE: To investigate the distribution and changes of blood copper, zinc, calcium, magnesium and iron in Chinese rural children aged 6 to 12 years in 2002 and 2012. METHODS: A total of 3420 blood samples were randomly selected from the China Nutrition and Health Survey 2002 and 2012. Metal concentrations in whole blood were determined by inductively coupled mass spectrometry(ICP-MS) after the dilution of 0. 5%(V/V) HNO_3 and 0. 05%(V/V) Triton-X-100. The changes between 2002 and 2012 of blood five elements concentration in children of 6-12 years in rural China were compared. RESULTS: The median of blood levels of copper, zinc, calcium, magnesium and iron in 2002 were 1. 02(95% CI 0. 79-1. 32), 4. 88(95% CI 3. 17-6. 77), 58. 59(95% CI 44. 97-74. 24), 41. 56(95% CI 33. 01-52. 28) and 423. 21(95% CI 338. 83-540. 69) mg/L, respectively. And in 2012, the median values were 1. 00(95% CI 0. 75-1. 31), 5. 12(95% CI 3. 17-7. 37), 63. 36(95% CI 41. 14-79. 16), 41. 78(95% CI 30. 89-52. 53) and 422. 06(95% CI 297. 83-522. 56) mg/L, respectively. There were significant differences in blood copper, zinc, calcium and iron levels in general, gender and age. There were also significant area differences in the distribution of copper, zinc, calcium, magnesium and iron, but the changes were opposite. CONCLUSION: In 2002 and 2012, there were differences in the blood distribution of Cu, Zn, Ca, Mg and Fe of children aged 6-12 in rural China in terms of general, gender, age and area.


Assuntos
Cálcio/sangue , Cobre/sangue , Ferro/sangue , Magnésio/sangue , Zinco/sangue , Criança , China , Feminino , Hemoglobinas/metabolismo , Humanos , Masculino , Espectrofotometria Atômica , Oligoelementos
17.
J Trace Elem Med Biol ; 54: 221-225, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31109616

RESUMO

In order to assess the individual trace element status of humans for either medical or scientific purposes, amongst others, blood serum levels are determined. Furthermore, animal models are used to study interactions of trace elements. Most published methods require larger amounts (500-1000 µL) of serum to achieve a reliable determination of multiple trace elements. However, oftentimes, these amounts of serum cannot be dedicated to a single analysis and the amount available for TE-determination is much lower. Therefore, a published ICP-MS/MS method for trace element determination in serum was miniaturized, optimized and validated for the measurement of Mn, Fe, Cu Zn, I and Se in as little as 50 µL of human and murine serum and is presented in this work. For validation, recoveries of multiple LOTs and levels from commercially available human reference serum samples were determined, intra- and inter-day variations were assessed and limits of detection and quantification determined. It is shown, that the method is capable of giving accurate and reproducible results for all six elements within the relevant concentration ranges for samples from humans living in central Europe as well as from laboratory mice. As a highlight, the achieved limits of detection and quantification for Mn were found to be at 0.02 µg/L serum and 0.05 µg/L serum, respectively, while using an alkaline diluent for the parallel determination of iodine.


Assuntos
Espectrometria de Massas em Tandem/métodos , Oligoelementos/sangue , Animais , Cobre/sangue , Humanos , Iodo/sangue , Ferro/sangue , Manganês/sangue , Camundongos , Selênio/sangue , Zinco/sangue
18.
J Trace Elem Med Biol ; 54: 226-231, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31109617

RESUMO

AIM: This review illustrates heavy metals toxicity, currently available therapies and the role and efficacy of chelation therapy for its management. SUMMARY: Heavy metals are necessary for various biological processes, but they become harmful in excess. Specifically, they induce oxidative stress by generating free radicals and reducing antioxidant levels. Heavy metals also alter the confirmation of protein and DNA and inhibit their function. Chelation therapy is commonly used to treat metals toxicity. Chelation is a chemical process that occurs when interaction between a central metal atom/ion and ligand leads to formation of a complex ring-like structure. The ligand has a donor ion/molecule, which has a lone pair of electrons and may be monodentate to polydentate. Each metal has a different reactivity with a ligand, so a specific chelation agent is required for each metal. Combination therapy with a chelating agent and an antioxidant led to improved outcome. CONCLUSION: Heavy metal poisoning is a common health problem because of mining, smelting, industrial, agricultural and sewage waste. Heavy metals can be efficiently excreted from the body following treatment with proper chelation agents.


Assuntos
Quelantes/uso terapêutico , Intoxicação por Metais Pesados/tratamento farmacológico , Metais Pesados/toxicidade , Arsênico/sangue , Cádmio/sangue , Cobre/sangue , Humanos , Ferro/sangue , Chumbo/sangue
19.
Ecotoxicol Environ Saf ; 178: 113-122, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-30999179

RESUMO

The rational of the current study was to assess whether Tribulus terrestris extract (TTE) could alleviate long-term copper (Cu) overload-induced testicular dysfunction compared to enalapril and losartan. Rats were administered either vehicle (control group, n = 10) or copper sulfate pentahydrate (CuSO4·5H2O, 200 mg/kg, p.o) for 90 days (n = 40). Cu-treated rats were randomized into four equal groups. One group was left untreated (Cu group) while the remaining three groups were daily co-treated with one of the following treatments along with CuSO4: TTE (10 mg/kg, p.o); enalapril (30 mg/kg, p.o); losartan (10 mg/kg, p.o). Excess Cu intake resulted in Cu overload coupled with a significant elevation in systolic blood pressure and serum angiotensin II levels along with a reduction in serum nitric oxide level. All concomitant treatments led to an alleviation of such deleterious effects. However, only losartan failed to ameliorate angiotensin II elevation. Additionally, all treatments protected the testes against Cu-overload-elicited zinc depletion and oxidative stress. Regarding reproductive function, the relative weights of testes, serum levels of testosterone and luteinizing hormone; the expression of steroidogenic genes; the protein levels of angiotensin II type 1 receptor and angiotensin converting enzyme 1, in addition to its activity, they were significantly reduced. Amongst all treatments, only TTE and E were able to revert these reproductive changes. In conclusion TTE and E were able to protect against Cu overload-induced impairment of testicular steroidogenesis. Thus, they might be considered as prophylactic drugs of choice against hypertension and testicular dysfunction to ameliorate Cu overload risk.


Assuntos
Angiotensinas/antagonistas & inibidores , Anti-Hipertensivos/farmacologia , Cobre/toxicidade , Extratos Vegetais/farmacologia , Espermatogênese/efeitos dos fármacos , Testículo/efeitos dos fármacos , Tribulus/química , Animais , Anti-Hipertensivos/isolamento & purificação , Pressão Sanguínea/efeitos dos fármacos , Cobre/sangue , Cobre/metabolismo , Enalapril/farmacologia , Fertilidade/efeitos dos fármacos , Losartan/farmacologia , Masculino , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Distribuição Aleatória , Ratos , Ratos Wistar , Testículo/metabolismo
20.
Molecules ; 24(7)2019 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-30959950

RESUMO

The aim of this paper was to review recent literature (from 2000 onwards) and summarize the newest findings on fluctuations in the concentration of some essential macro- and microelements in those patients with a history of chronic alcohol abuse. The focus was mainly on four elements which the authors found of particular interest: Iron, magnesium, copper, and manganese. After independently reviewing over 50 articles, the results were consistent with regard to iron and magnesium. On the other hand, data were limited, and in some cases contradictory, as far as copper and manganese were concerned. Iron overload and magnesium deficiency are two common results of an excessive and prolonged consumption of alcohol. An increase in the levels of iron can be seen both in the serum and within the cells, hepatocytes in particular. This is due to a number of factors: Increased ferritin levels, lower hepcidin levels, as well as some fluctuations in the concentration of the TfR receptor for transferrin, among others. Hypomagnesemia is universally observed among those suffering from alcoholism. Again, the causes for this are numerous and include malnutrition, drug abuse, respiratory alkalosis, and gastrointestinal problems, apart from the direct influence of excessive alcohol intake. Unfortunately, studies regarding the levels of both copper and manganese in the case of (alcoholic) liver disease are scarce and often contradictory. Still, the authors have attempted to summarize and give a thorough insight into the literature available, bearing in mind the difficulties involved in the studies. Frequent comorbidities and mutual relationships between the elements in question are just some of the complications in the study of this topic.


Assuntos
Alcoolismo/sangue , Cobre/sangue , Ferro/sangue , Magnésio/sangue , Manganês/sangue , Alcoolismo/metabolismo , Biomarcadores , Encéfalo/metabolismo , Suscetibilidade a Doenças , Metabolismo Energético , Humanos , Fígado/metabolismo , Especificidade de Órgãos
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