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1.
Meat Sci ; 160: 107963, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31693966

RESUMO

Quality of bovine longissimus lumborum, infraspinatus and cleidooccipitalis muscles after high-intensity ultrasound (HIU; 40 kHz and a power of 11 W/cm2 for 0, 40, 60, and 80 min) and aging (0, 7 and 14 d) was evaluated. The effects of HIU on pH and color of meat were not considered negative. HIU improved water holding capacity (WHC) of l.lumborum and infraspinatus only after aging. Whereas, the WHC of cleidooccipitalis increased immediately after sonication. The total collagen of HIU treated samples was significantly lower compared to the untreated samples. Ultrasonication for 80 min was the most effective for infraspinatus and cleidooccipitalis. Toughness decreased with HIU, iii nfraspinatus and l.lumborum tenderized more than cleidooccipitalis. HIU application and 7 d aging is an excellent combined treatment to improve tenderness of the three muscles. Infraspinatus was the most tender meat. HIU could help industry to improve the quality of beef as it helps in tenderization and accelerates maduration particularly of l.lumborum.


Assuntos
Manipulação de Alimentos/métodos , Carne Vermelha/análise , Ondas Ultrassônicas , Animais , Bovinos , Colágeno/análise , Cor , Concentração de Íons de Hidrogênio , Músculo Esquelético/química , Resistência ao Cisalhamento , Água
2.
Acta Cir Bras ; 34(9): e201900901, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800678

RESUMO

PURPOSE: To evaluate the effects of tadalafil (TD) in preventing histological alterations of the corpus cavernosum caused by isolated lesions of cavernous nerve (ILCN) and artery (ILCA) in rats. METHODS: Fifty male Wistar rats were randomly assigned in five groups: G1: control; G2: bilateral ILCN; G3: bilateral ILCA; G4: ILCN+TD; G5: ILCA+TD. The cavernous bodies were submitted to histomorphometry, immunohistochemistry and biochemical analysis. RESULTS: Nerve density was significantly higher in G2 and G4 compared to control (22.62±2.84 and 19.53±3.47 vs. 15.72±1.82; respectively, p<0.05). Smooth muscle density was significantly lower in G2 and G3 in comparison to G1 (12.87±1.90 and 18.93±1.51 vs. 21.78±1.81, respectively; p<0.05). A significant decrease in the sinusoidal lumen area was observed in G2 compared to controls (5.01±1.62 vs. 9.88±3.66, respectively; p<0.05) and the blood vessel density was increased in G2 and G3 (29.32±4.13 e 20.80±2.47 vs. 10.13±2.71, p<0.05). Collagen density was higher in G3 compared to G1 (93.76±15.81 vs. 64.59±19.25; p<0.05). CONCLUSIONS: Histomorphometric alterations caused by ILCN were more intense than those produced by vascular injury, but the collagen analyses showed more fibrosis in animals with ILCA. TD was effective in preventing the majority of the alterations induced by the periprostatic bundle injury.


Assuntos
Pênis/irrigação sanguínea , Pênis/inervação , Traumatismos dos Nervos Periféricos/prevenção & controle , Inibidores da Fosfodiesterase 5/farmacologia , Substâncias Protetoras/farmacologia , Tadalafila/farmacologia , Animais , Colágeno/análise , Colágeno/efeitos dos fármacos , Tecido Elástico/anatomia & histologia , Tecido Elástico/efeitos dos fármacos , Disfunção Erétil/prevenção & controle , Imuno-Histoquímica , Masculino , Pênis/efeitos dos fármacos , Pênis/patologia , Prostatectomia/efeitos adversos , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes
3.
Acta Cir Bras ; 34(10): e201901005, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31851213

RESUMO

PURPOSE: To quantify and compare the expression of stromal elements in prostate adenocarcinoma of different Gleason scores with non-tumor area (control). METHODS: We obtained 132 specimens from samples of prostate peripheral and transition zone. We analyzed the following elements of the extracellular matrix: collagen fibers, elastic system, smooth muscle fibers and blood vessels. The tumor area and non-tumor area (control) of the TMA (tissue microarray) were photographed and analyzed using the ImageJ software. RESULTS: The comparison between the tumor area and the non-tumor area showed significant differences between stromal prostate elements. There was an increase of collagen fibers in the tumor area, mainly in Gleason 7. Elastic system fibers showed similar result, also from the Gleason 7. Blood vessels showed a significant increase occurred in all analyzed groups. The muscle fibers exhibited a different behavior, with a decrease in relation to the tumor area. CONCLUSIONS: There is a significant difference between the extracellular matrix in prostate cancer compared to the non-tumor area (control) especially in Gleason 7. Important modifications of the prostatic stromal elements strongly correlate with different Gleason scores and can contribute to predict the pathological staging of prostate cancer.


Assuntos
Adenocarcinoma/patologia , Neoplasias da Próstata/patologia , Células Estromais/patologia , Idoso , Idoso de 80 Anos ou mais , Vasos Sanguíneos/patologia , Colágeno/análise , Tecido Elástico/anatomia & histologia , Humanos , Masculino , Pessoa de Meia-Idade , Músculo Liso/patologia , Gradação de Tumores , Valores de Referência , Estudos Retrospectivos , Análise Serial de Tecidos
4.
Isotopes Environ Health Stud ; 55(5): 478-498, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31402710

RESUMO

Faecal isotopic analysis may complement other non-invasive wildlife survey tools for monitoring landscape use by carnivores, such as motion-detecting cameras and non-invasive genetic sampling. We analysed carbon, nitrogen, and strontium isotopes in faecal matter produced by jaguars (Panthera onca) as well as bones from consumed prey at the Mountain Pine Ridge Forest Reserve (MPR) in Belize, Central America. The MPR is ideally suited for a spatial isotope study as vegetation and geology both vary considerably. The isotopic composition of faecal matter should reflect the habitat and geology where consumed prey lived. We used bone from consumed prey recovered from jaguar scats as a proxy for diet. Faecal matter and bone showed comparable spatial isotopic trends, suggesting that the isotopic composition of jaguar faeces can be used to detect foraging in different habitats (pine forest versus broadleaf forest) or on different geologies (Mesozoic carbonates; Palaeozoic granite, contact metamorphics, and metasediments). This result is reassuring as bones are not always present in carnivore scats. Studying landscape use by cryptic and wide-ranging carnivore species like jaguars remains challenging. Isotopic analysis of faecal matter complements the existing array of non-invasive spatial monitoring tools.


Assuntos
Isótopos de Carbono/análise , Fezes/química , Isótopos de Nitrogênio/análise , Panthera/fisiologia , Comportamento Predatório , Animais , Belize , Osso e Ossos/química , Carnivoridade/fisiologia , Colágeno/análise , Colágeno/química , Ecossistema , Isótopos de Estrôncio/análise
5.
Khirurgiia (Mosk) ; (8): 12-16, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31464268

RESUMO

OBJECTIVE: To define the indication for preventive mesh implantation in patients scheduled for laparotomy. MATERIAL AND METHODS: There were 108 patients divided into the control (52 patients without anterior abdominal wall hernia) and the main (56 patients with ventral hernia) groups. RESULTS: Predominance of collagen type III over type I was observed in patients older 60 years in the main group. The maximum ratio of collagen types I and III was 1.4 in the main group. The minimum number of inter-fiber spaces (n=5) was noted in patients aged 30-40 years. The maximum density of connective tissue was 250 pixels per inch. CONCLUSION: Analysis of connective tissue structures revealed some important age-related features and markers of connective tissue dysplasia in the main group. An indication for preventive mesh implantation in patients scheduled for laparotomy was determined.


Assuntos
Colágeno/análise , Doenças do Tecido Conjuntivo/complicações , Hérnia Ventral/prevenção & controle , Hérnia Incisional/prevenção & controle , Laparotomia/efeitos adversos , Telas Cirúrgicas , Parede Abdominal/patologia , Parede Abdominal/cirurgia , Adulto , Tecido Conjuntivo/química , Tecido Conjuntivo/patologia , Doenças do Tecido Conjuntivo/patologia , Hérnia Ventral/etiologia , Hérnia Ventral/patologia , Herniorrafia/métodos , Humanos , Hérnia Incisional/etiologia , Hérnia Incisional/patologia , Pessoa de Meia-Idade
6.
Arq. bras. med. vet. zootec. (Online) ; 71(4): 1331-1338, jul.-ago. 2019. tab, graf
Artigo em Português | LILACS, VETINDEX | ID: biblio-1038606

RESUMO

Avaliou-se a incidência da miopatia White Striping (WS) em três linhagens de frangos de corte machos e suas consequências sobre a composição química e a qualidade física da carne. Para determinação das porcentagens de incidência dos diferentes graus da miopatia WS (normal, moderada e severa) nas linhagens, foi utilizado um delineamento inteiramente ao acaso (DIC), com três tratamentos (linhagens A, B e C) e cinco repetições de 300 peitos cada. Para as variáveis químicas e físicas, utilizou-se um DIC em esquema fatorial 3 x 3 (três linhagens x três níveis de WS), com 15 repetições cada. Os dados foram submetidos à análise de variância e, em casos de diferenças significativas, utilizou-se o teste de Tukey (P≤0,05). As aves da linhagem A apresentaram 84,81% de peitos classificados como normais e 9,91% de peitos classificados como moderados, valores respectivamente maiores e menores que nas duas demais linhagens. Os peitos classificados com miopatia WS severa foram mais pesados e apresentaram maiores porcentagens de lipídios e umidade, e menor concentração de proteína bruta. As concentrações de colágeno total, termo solúvel e insolúvel variaram com o nível de acometimento por WS nos filés, com comportamento específico para cada linhagem.(AU)


The incidence of the White Striping (WS) myopathy in three male broiler strains and its consequences on the chemical composition and physical quality of the meat was evaluated. A completely randomized design (DIC) with three treatments (strains A, B and C) and five replicates of 300 breasts each were used to determine the incidence rates of the different degrees of WS myopathy (normal, moderate and severe) in each strain. For the chemical and physical variables, a 3 X 3 factorial scheme was used (three strains X three WS levels), with 15 replicates. The data were submitted to ANOVA and in cases of significant differences the Tukey test (P< 0.05) was used.The A strain chickens presented 84.81% of breasts classified as normal and 9.91% of breasts classified as moderate, respectively higher and lower values than in the other two strains. Breasts classified with severe WS myopathy were heavier and had higher percentages of lipids and moisture, and lower concentration of crude protein. However, the concentrations of total, soluble and insoluble collagen had interaction effects between lineage and WS level in the fillets, and therefore, the same behavior was not observed in the samples of the different strains.(AU)


Assuntos
Animais , Galinhas , Colágeno/análise , Músculo Estriado , Carne/análise , Doenças Musculares/veterinária , Qualidade dos Alimentos
7.
Org Biomol Chem ; 17(31): 7380-7387, 2019 08 07.
Artigo em Inglês | MEDLINE | ID: mdl-31342036

RESUMO

To develop a facile method for detecting denatured collagen, we investigated the structure-activity relationship of cyclic collagen-mimetic peptides (cCMPs). Reported cCMP prototypes tend to self-assemble and they must be disassembled just before use. Introducing charge repulsion and a deformation in the peptide backbone structure enabled cCMPs to detect denatured collagen without a pre-treatment for disassembly. Using the optimized cCMP, types I-V collagen were detected by western blotting and denatured collagen fibrils were visualized in a cell culture system.


Assuntos
Colágeno/análise , Peptídeos Cíclicos/química , Animais , Células Cultivadas , Fibroblastos/química , Camundongos , Relação Estrutura-Atividade
8.
Int J Mol Med ; 44(2): 549-558, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31198980

RESUMO

Hydrogen sulfide (H2S) has antifibrotic activity in the kidneys, heart, lungs, and other organs. The present study investigated the protective activity of exogenous H2S against myocardial fibrosis in a rat model of diabetes. Animals were assigned to normal control, diabetes mellitus (DM), DM + sodium hydrosulfide (NaHS; DM + NaHS) and NaHS groups. Fasting blood glucose (FBG), cardiac function and hydroxyproline were monitored. Heart histomorphology and ultrastructure were additionally evaluated. Wnt1­inducible signaling pathway protein (WISP)­1 protein expression in the myocardium was determined by immunohistochemical staining. Matrix metalloprotease (MMP)­2, tissue inhibitor of metalloproteinase (TIMP)­2, collagens, and canonical Wnt and transforming growth factor (TGF)­ß1/SMAD family member 3 (Smad3) pathway­related proteins were assessed by western blotting. Cardiac function was decreased, and myocardial injury, hypertrophy and fibrosis were increased in the diabetes model rats. MMP­2 expression was decreased, and the expressions of WISP­1, TIMP­2, collagens, and canonical Wnt and TGF­ß1/Smad3 pathway­related proteins were increased in the myocardia of the diabetes model rats. The present results indicated that the canonical Wnt pathway promoted diabetic myocardial fibrosis by upregulating the TGF­ß1/Smad3 pathway. Except for FBG, exogenous H2S ameliorated the changes in diabetes­associated indices in rats in the DM + NaHS group. The results are consistent with H2S protection of streptozotocin­induced myocardial fibrosis in the diabetes model rats by downregulation of the canonical Wnt and TGF­ß1/Smad3 pathway and decreased myocardial collagen deposition.


Assuntos
Diabetes Mellitus Experimental/complicações , Cardiopatias/tratamento farmacológico , Coração/efeitos dos fármacos , Sulfeto de Hidrogênio/uso terapêutico , Miocárdio/patologia , Via de Sinalização Wnt/efeitos dos fármacos , Animais , Colágeno/análise , Colágeno/metabolismo , Fibrose , Cardiopatias/metabolismo , Cardiopatias/patologia , Masculino , Miocárdio/metabolismo , Ratos Sprague-Dawley
9.
Poult Sci ; 98(8): 3268-3277, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31041445

RESUMO

Broiler chicks in the immediate posthatch handling period are exposed to thermal stress, with potentially harmful consequences for muscle growth and structure (e.g., less protein and more fat deposition). We addressed the effects of broiler chicks' exposure to various ambient temperatures during the first 13 D posthatch on their performance, as well as on muscle development and structure, up to day 35. Body weight and pectoralis muscle growth were lower throughout the entire period in the high-heat-exposed chicks (39°C, Hot) and to a lesser extent in the mild-heat-exposed chicks (35°C, Hot Mild) than in the Control chicks that were raised under a commercial protocol. In the cold-exposed chicks (29oC, Cold), BW and pectoralis muscle absolute growth were similar to the Control group throughout the entire period. The lower body and muscle growth in the Hot and Hot Mild groups were reflected in a lower number of myonuclei expressing proliferating cell nuclear cell in pectoralis major muscle cross sections sampled on day 8, in the distribution of myofibers as the experiment progressed, and in mean myofiber diameter on day 35, whereas in the Cold group, these numbers exceeded that of the Control group. However, TUNEL assay revealed similar cell survival in all groups. Hematoxylin-eosin and Oil red O staining revealed the highest fat deposition in the pectoralis muscle derived from the Hot group, whereas lower fat deposition was observed in the Control Cold group. These results were corroborated by immunostaining for CCAAT/enhancer binding protein ß in the pectoralis muscle, the levels of which were significantly higher in the Hot and Hot Mild groups on day 35 than in the Control group. Similar results were observed with Sirius red staining for collagen content in the pectoralis muscle. Together, the results imply long-term effects of chronic heat stress vs. cold stress in the early posthatch period on the broiler's body and muscle growth in general and myodegeneration of the pectoralis muscle in particular.


Assuntos
Galinhas/crescimento & desenvolvimento , Resposta ao Choque Frio/fisiologia , Resposta ao Choque Térmico/fisiologia , Músculos Peitorais/crescimento & desenvolvimento , Animais , Animais Recém-Nascidos/fisiologia , Peso Corporal , Galinhas/fisiologia , Temperatura Baixa/efeitos adversos , Colágeno/análise , Temperatura Alta/efeitos adversos , Masculino , Desenvolvimento Muscular/fisiologia , Miofibrilas , Músculos Peitorais/química , Estresse Fisiológico
10.
J Orthop Res ; 37(9): 2004-2018, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31041999

RESUMO

Fibrosis may be a key factor in sensorimotor dysfunction in patients with chronic overuse-induced musculoskeletal disorders. Using a clinically relevant rodent model, in which performance of a high demand handle-pulling task induces tissue fibrosis and sensorimotor declines, we pharmacologically blocked cellular communication network factor 2 (CCN2; connective tissue growth factor) with the goal of reducing the progression of these changes. Young adult, female Sprague-Dawley rats were shaped to learn to pull at high force levels (10 min/day, 5 weeks), before performing a high repetition high force (HRHF) task for 3 weeks (2 h/day, 3 days/week). HRHF rats were untreated, or treated in task weeks 2 and 3 with a monoclonal antibody that blocks CCN2 (FG-3019), or a control immunoglobulin G (IgG). Control rats were untreated or received FG-3019, IgG, or vehicle (saline) injections. Mean task reach rate and grasp force were higher in 3-week HRHF + FG-3019 rats, compared with untreated HRHF rats. Grip strength declined while forepaw mechanical sensitivity increased in untreated HRHF rats, compared with controls; changes improved by FG-3019 treatment. The HRHF task increased collagen in multiple tissues (flexor digitorum muscles, nerves, and forepaw dermis), which was reduced with FG-3019 treatment. FG-3019 treatment also reduced HRHF-induced increases in CCN2 and transforming growth factor ß in muscles. In tendons, FG-3019 reduced HRHF-induced increases in CCN2, epitendon thickening, and cell proliferation. Our findings indicate that CCN2 is critical to the progression of chronic overuse-induced multi-tissue fibrosis and functional declines. FG-3019 treatment may be a novel therapeutic strategy for overuse-induced musculoskeletal disorders. © 2019 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res 37:2004-2018, 2019.


Assuntos
Fator de Crescimento do Tecido Conjuntivo/fisiologia , Transtornos Traumáticos Cumulativos/etiologia , Transtornos Neurológicos da Marcha/prevenção & controle , Animais , Doença Crônica , Colágeno/análise , Fator de Crescimento do Tecido Conjuntivo/análise , Fator de Crescimento do Tecido Conjuntivo/antagonistas & inibidores , Transtornos Traumáticos Cumulativos/tratamento farmacológico , Modelos Animais de Doenças , Feminino , Fibrose , Força da Mão , Inflamação/etiologia , Ratos , Ratos Sprague-Dawley , Fator de Crescimento Transformador beta1/análise
11.
Clinics (Sao Paulo) ; 74: e829, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31038563

RESUMO

OBJECTIVE: To test whether swimming training benefits femoral neck strength in young diabetic rats under insulin therapy. METHODS: A total of 60 male Wistar rats (age: 40 days) were divided equally into the following six groups: control sedentary, control exercise, diabetic sedentary, diabetic exercise, diabetic sedentary plus insulin and diabetic exercise plus insulin. Diabetes was induced with a unique intraperitoneal injection (60 mg/kg body weight) of streptozotocin. Seven days after the injection and after 12 hours of fasting, the animals with blood glucose levels ≥300 mg/dL were considered diabetic. Seven days after the induction of diabetes, the animals in the exercise groups were subjected to progressive swimming training (final week: 90 min/day; 5 days/week; 5% load) for eight weeks. The animals in the insulin groups received a daily dose of insulin (2-4 U/day) for the same period. RESULTS: Severe streptozotocin-induced diabetes reduced the structural properties of the femoral neck (trabecular bone volume, trabecular thickness and collagen fiber content). The femoral neck mechanical properties (maximum load and tenacity) were also impaired in the diabetic rats. Insulin therapy partially reversed the damage induced by diabetes on the structural properties of the bone and mitigated the reductions in the mechanical properties of the bone. The combination of therapies further increased the femoral neck trabecular bone volume (∼30%), trabecular thickness (∼24%), collagen type I (∼19%) and type III (∼13%) fiber contents, maximum load (∼25%) and tenacity (∼14%). CONCLUSIONS: Eight weeks of swimming training potentiates the recovery of femoral neck strength in young rats with severe streptozotocin-induced diabetes under insulin therapy.


Assuntos
Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Experimental/fisiopatologia , Terapia por Exercício/métodos , Colo do Fêmur/fisiopatologia , Hipoglicemiantes/uso terapêutico , Insulina/uso terapêutico , Natação/fisiologia , Animais , Glicemia/análise , Peso Corporal/fisiologia , Osso Esponjoso/fisiopatologia , Colágeno/análise , Fraturas Ósseas/fisiopatologia , Fraturas Ósseas/prevenção & controle , Masculino , Condicionamento Físico Animal/fisiologia , Ratos Wistar , Reprodutibilidade dos Testes , Estreptozocina , Fatores de Tempo
12.
Mar Drugs ; 17(5)2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31052462

RESUMO

Collagen was extracted from bigeye tuna (Thunnus obesus) skins by salting-out (PSC-SO) and isoelectric precipitation (PSC-IP) methods. The yield of the PSC-IP product was approximately 17.17% (dry weight), which was greater than the yield obtained from PSC-SO (14.14% dry weight). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that collagen from bigeye tuna skin belongs to collagen type I. Inductively coupled plasma mass spectrometry results indicate that the heavy metal abundance in PSC-IP was lower than the maximum acceptable amounts according to Chinese regulatory standards. In addition, results from a methylthiazolyldiphenyl-tetrazolium bromide assay and an in vitro scratch assay demonstrated that PSC-IP could promote the proliferation and migration of NIH-3T3 fibroblasts. Overall, results suggest PSC-IP could be used to rapidly extract collagen from marine by-products instead of traditional salting-out methods. Collagen from bigeye tuna skin may also have strong potential for cosmetic and biomedical applications.


Assuntos
Colágeno/análise , Colágeno/isolamento & purificação , Proteínas de Peixes/análise , Proteínas de Peixes/isolamento & purificação , Animais , Ensaios de Migração Celular , Proliferação de Células , Colágeno/química , Colágeno Tipo I , Proteínas de Peixes/química , Camundongos , Células NIH 3T3/efeitos dos fármacos , Pele/química , Pele/metabolismo , Solubilidade , Atum
13.
Artif Organs ; 43(10): 976-987, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31140630

RESUMO

Transcatheter aortic valve implantation (TAVI) has gained considerable acceptance in the past decade due to its lower risks than conventional open-heart surgery. However, the deformation and delamination of the leaflets during the crimping procedure have raised questions about the durability and long-term serviceability of the pericardium tissue from which the leaflets are made. The collagen architecture, wall thickness and mechanical properties of donkey pericardium were investigated to assess its suitability as an alternative material for the manufacture of heart valves. Coupons sampled from different locations of donkey pericardium were investigated. Bovine, equine, and porcine pericardium specimens served as controls. The donkey pericardium had a similar surface morphology to that of the control pericardia except for the wavy topology on both the fibrous and serous sides. The average thickness of donkey pericardium (ca. 120 µm) was significantly lower than that from bovine (375 µm) and equine (410 µm), but slightly higher than that from porcine (99 µm) specimens. The interlaced wavy collagen bundles in the pericardium were composed of collagen fibers about 100 nm in diameter. This unique structure ensures that the donkey pericardium has a comparable ultimate tensile strength (UTS) and a much higher failure strain than the commercial pericardia used for the manufacture of heart valves. The donkey pericardium has an organized wavy collagen bundle architecture similar to that of bovine pericardium and has a satisfactory UTS and high failure strain. The thin and strong donkey pericardium might be a good candidate valve leaflet material for TAVI.


Assuntos
Materiais Biocompatíveis/química , Bioprótese , Colágeno/análise , Próteses Valvulares Cardíacas , Pericárdio/química , Animais , Valva Aórtica/cirurgia , Fenômenos Biomecânicos , Bovinos , Elasticidade , Equidae , Valvas Cardíacas/cirurgia , Cavalos , Teste de Materiais , Pericárdio/ultraestrutura , Suínos , Resistência à Tração , Substituição da Valva Aórtica Transcateter
14.
Histochem Cell Biol ; 152(2): 145-153, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31144029

RESUMO

Reference data are lacking on the periodontal ligament and the gingival tissue of the rat model, which would be useful for studies of new medical or biomaterial periodontal treatments. The objective of the current study was to propose cellular and collagen reference values of gingival and periodontal ligament tissues in rat, using a simple and reliable quantitative method after decalcification. Mandibular samples of ten adult Sprague-Dawley rats were used. Mild decalcification was carried out using ethylenediaminetetraacetic acid (EDTA) to preserve the morphology of tissues. Half of the samples were decalcified and the other half were not. The gingiva and the periodontal ligament were analyzed. Descriptive histology and computer-assisted image analysis were performed. The data showed that qualitatively, cellular and extracellular matrix morphologies were well preserved compared to non-decalcified periodontal soft tissue biopsies. Histomorphometrically, constitutive cellularity and the total amount of native collagen, collagen directionality and collagen anisotropy in both experimental conditions did not significantly differ. Taken together, these results suggested that EDTA decalcification did not negatively affect the studied endpoints. Moreover, this mild decalcification method allowed in situ maintenance of the periodontal soft and hard tissue integrity. The structural and compositional computerized assessment performed in the healthy periodontal soft tissue could provide reference values that will be required for future assessment on the effects of pathological, reparative and regenerative processes in rat periodontal soft tissues.


Assuntos
Colágeno/análise , Gengiva/química , Ligamento Periodontal/química , Animais , Anisotropia , Colágeno/normas , Gengiva/citologia , Masculino , Ligamento Periodontal/citologia , Projetos Piloto , Conformação Proteica , Ratos , Ratos Sprague-Dawley , Valores de Referência
15.
Meat Sci ; 155: 27-35, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31059939

RESUMO

The objective of the present experiment was to evaluate the effects of encapsuled active principles (eugenol, thymol and vanillin blend), and clove and rosemary essential oils inclusion into the finishing diets of Nellore heifers on chemical composition, collagen content, fatty acid profile and structural traits (fibers types, type I and III collagen and sarcomere length) of longissimus muscle. Treatments had no effect (P > .05) on type of fiber, the meat chemical composition or in the muscle fatty acid profile. However, the diet with clove and rosemary essential oil and the active principle blend led to an increase in sarcomere length, higher soluble collagen content and a lower amount of type III collagen (P < .05). The mixture of both active principles and essential oils clove and cinnamon essential oil, have a potential use in animal feed, favoring a greater sarcomere length, that is directly related to the increase of the meat tenderness, without altering the meat chemical composition or fatty acid profile.


Assuntos
Ração Animal/análise , Colágeno/análise , Ácidos Graxos/análise , Óleos Voláteis/farmacologia , Carne Vermelha/análise , Animais , Benzaldeídos/farmacologia , Bovinos , Dieta/veterinária , Eugenol/farmacologia , Feminino , Fibras Musculares Esqueléticas/efeitos dos fármacos , Sarcômeros/efeitos dos fármacos , Syzygium , Timol/farmacologia
16.
Int J Paleopathol ; 25: 46-55, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31051405

RESUMO

OBJECTIVE: This paper aims at investigating the possible existence of isotopic offsets in δ13Ccol and δ15Ncol values in relation to tertiary syphilis. MATERIAL: Based on materials from the 19th c. A.D. deriving from the pathological-anatomical reference collection (the Jedlicka collection) of the National Museum in Prague (Czech Republic), a comparative approach of ten individuals with syphilis and nine without the disease was undertaken. METHODS: Bone powder samples were defatted according to the protocol of Liden et al. (1995). Bone collagen was extracted following the protocol of Bocherens et al. (1991). RESULTS: Our results show that individuals with syphilis have lower δ13Ccol values than individuals without the disease; the observed difference between the two groups is about 0.3-0.4‰, which is relatively small but still meaningful. However, no difference between δ15Ncol values of the two groups has been noticed. CONCLUSIONS: Either diets prescribed by physicians to syphilitic patients or nutritional stress caused by cyclic appetite disturbance due to the disease itself or the administered medical treatment appeared to be possible explanations of the observed isotopic pattern. Overall, the response of the two isotopic proxies could argue for relatively limited nutritional restrictions. SIGNIFICANCE: This is the first study examining bone collagen isotopic response to syphilis based on clinically documented human skeletal materials. LIMITATIONS: The sample sizes are relatively small and cautiousness must be taken regarding the interpretations of the data. SUGGESTIONS FOR FURTHER RESEARCH: Compound-specific stable isotope investigations and analysis of mercury content could be helpful to better understand the observed isotopic effects.


Assuntos
Colágeno/análise , Sífilis/história , Adolescente , Adulto , Idoso , Osso e Ossos/química , Osso e Ossos/patologia , Isótopos de Carbono/análise , República Tcheca , Dieta , Feminino , História do Século XIX , Humanos , Masculino , Pessoa de Meia-Idade , Museus , Isótopos de Nitrogênio/análise , Sífilis/patologia , Adulto Jovem
17.
Biol Res ; 52(1): 23, 2019 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-30992080

RESUMO

BACKGROUND: Conjunctival filtering bleb scar formation is the main reason for the failure of glaucoma filtration surgery. Cytoglobin (Cygb) has been reported to play an important role in extracellular matrix (ECM) remodeling, fibrosis and tissue damage repairing. This study aimed to investigate the role of Cygb in anti-scarring during excessive conjunctival wound healing after glaucoma filtration surgery. METHODS: Cygb was overexpressed in human tenon fibroblasts (hTFs) by transfecting hTFs with lentiviral particles encoding pLenti6.2-FLAG-Cygb. Changes in the mRNA and protein levels of fibronectin, collagen I, collagen III, TGF-ß1, and HIF1α were determined by RT-PCR and western blotting respectively. RESULTS: After Cygb overexpression, hTFs displayed no significant changes in visual appearance and cell counts compared to controls. Whereas, Cygb overexpression significantly decreased the mRNA and protein expression levels of collagen I, collagen III and fibronectin compared with control (p < 0.01). There was also a statistically significant decrease in the mRNA and protein levels of TGF-ß1 and HIF-1α in hTFs with overexpressed Cygb compared with control group (p < 0.05). CONCLUSION: Our study provided evidence that overexpression of Cygb decreased the expression levels of fibronectin, collagen I, collagen III, TGF-ß1 and HIF-1α in hTFs. Therefore, therapies targeting Cygb expression in hTFs may pave a new way for clinicians to solve the problem of post-glaucoma surgery scarring.


Assuntos
Citoglobina/metabolismo , Matriz Extracelular/metabolismo , Fibroblastos/metabolismo , Cápsula de Tenon/metabolismo , Colágeno/análise , Citoglobina/farmacologia , Matriz Extracelular/efeitos dos fármacos , Fibronectinas/análise , Humanos , RNA Mensageiro/análise , Fator A de Crescimento do Endotélio Vascular/metabolismo
18.
Spectrochim Acta A Mol Biomol Spectrosc ; 218: 243-247, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-31003049

RESUMO

Osteoarthritis (OA) is not only related to the degradation of articular cartilage, but also possibly to the changes of subchondral bone. The purpose of this study was to assess whether specific differences could be resolved from bone composition, as also contributed to OA. These differences were assessed by using Fourier transform infrared spectroscopy (FTIRS). The main parameters including mineral content, carbonate content, crystallinity, collagen cross-linking ratio (XLR) and acid phosphate content were represented with characteristic peak integration. It was found that mineral and carbonate content varied significantly with depths at different OA stages. Mineral content increased with depth in healthy samples, while carbonate content showed opposite trend. The mineral content reduced obviously with OA duration, which was different with carbonate decreasing only at early stage of OA. In addition, the content of acid phosphate, collagen maturity (XLR) and crystallinity slight varied with the OA aggravation. Therefore, the changes in subchondral bone were significantly associated with cartilage degeneration and OA, the associated parameters should be targeted for OA therapies.


Assuntos
Osso e Ossos/patologia , Osteoartrite/patologia , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Animais , Osso e Ossos/química , Carbonatos/análise , Colágeno/análise , Cães , Durapatita/análise , Minerais/análise , Fosfatos/análise
19.
Rapid Commun Mass Spectrom ; 33(15): 1258-1266, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-30993809

RESUMO

RATIONALE: The use of multi-isotopic analysis (δ15 N, δ13 C and δ34 S values) of archaeological bone collagen to assist in the interpretation of diet, movement and mobility of prehistoric populations is gradually increasing, yet many researchers have traditionally avoided investigating sulphur due to its very low concentrations (<0.3%) in mammalian collagen. For this reason, and as a consequence of analytical detection limits, sulphur is usually measured separately from carbon and nitrogen, which leads to longer analytical times and higher costs. METHODS: A Thermo Scientific™ EA IsoLink™ isotope ratio mass spectrometry (IRMS) system, with the ability to rapidly heat a gas chromatography (GC) column and concentrate the sample gas online without cryo-trapping, was used at the Radiocarbon Laboratory at the Scottish Universities Environmental Research Centre (SUERC). Optimisation of the GC temperature and carrier gas flow rate in the elemental analyser resulted in improved signal-to-noise (S/N) ratio and sensitivity for SO2 . This allowed for routine sequential N2 , CO2 and SO2 measurements on small samples of bone collagen. RESULTS: Improvements in sample gas transfer to the mass spectrometer allows for sequential δ15 N, δ13 C and δ34 S values to be measured in 1-1.5 mg samples of bone collagen. Moreover, the sensitivity and S/N ratio of the sample gas, especially SO2 , is improved, resulting in precisions of ±0.15‰ for δ15 N values, ±0.1‰ for δ13 C values and ±0.3‰ for δ34 S values. Previous instrumentation allowed for the analysis of ~30 unknown samples before undertaking maintenance; however, ~150 unknown samples can now be measured, meaning a 5-fold increase in sample throughput. CONCLUSIONS: The ability to sequentially measure δ15 N, δ13 C and δ34 S values rapidly in archaeological bone collagen is an attractive option to researchers who want to build larger, more succinct datasets for their sites of interest, at a much-reduced analytical cost and without destroying larger quantities of archaeological material.


Assuntos
Arqueologia/métodos , Osso e Ossos/química , Isótopos de Carbono/análise , Colágeno/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Isótopos de Nitrogênio/análise , Isótopos de Enxofre/análise , Animais , Escócia , Universidades
20.
Med Sci Monit ; 25: 2377-2385, 2019 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-30936416

RESUMO

BACKGROUND In this study we prepared liposome microbubbles loading resveratrol (LMLR) and evaluated its therapeutic effect on injury of gastrocnemius muscle in rats. MATERIAL AND METHODS LMLR was prepared and characterized by particle size, potential, and microscopy, and a rat model of acute blunt injury of gastrocnemius muscle was established. After treatments with resveratrol or LMLR, the therapeutic effects were evaluated by hematoxylin-eosin (HE) staining. The expression of MHCIIB and vimentin in mRNA level was measured by real-time PCR. The expression of desmin and collagen I protein was assessed by immunohistochemistry. RESULTS LMLR showed regular cycle shape in a size of ~1000 nm. LMLR was negatively charged (-30 mV). The in vitro release of LMLR was close to 80% at 10 h and 90% at 48 h. Acute gastrocnemius muscle injury was established in rats and tissue recovery was observed after LMLR treatment as evidenced by HE staining, decreased expression of MHCIIB, and increased expression of vimentin. Moreover, LMLR treatment obviously facilitated desmin expression and reduced collagen I expression. CONCLUSIONS LMLR is effective in treating acute blunt injury of gastrocnemius muscle in rats.


Assuntos
Músculo Esquelético/efeitos dos fármacos , Resveratrol/administração & dosagem , Resveratrol/farmacologia , Animais , Colágeno/análise , Desmina/análise , Sistemas de Liberação de Medicamentos/métodos , Lipossomos/farmacologia , Masculino , Microbolhas/uso terapêutico , Modelos Animais , Ratos , Ratos Sprague-Dawley , Resveratrol/metabolismo
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