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1.
Food Chem ; 337: 127762, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32777563

RESUMO

Gelatin and collagen are considered halal-critical ingredients as they are typically derived from either bovine or porcine animals. Current analytical methods for determining the sources of gelatin and collagen suffer from limitations in terms of robustness and false positives in peptide matching. Thus, the aim of this study was to investigate the utility of monitoring hydroxyproline, a signature amino acid for gelatin and collagen, for identifying potentially haram foodstuffs. To determine the hydroxyproline profiles among animal- and plant-based samples, one-way univariate analysis of variance followed by pair-wise comparison was used to establish statistical significance. Multivariate chemometric analysis through principal component analysis revealed a discrete distribution pattern among 59 samples due to hydroxyproline variability. Finally, inter- and intra-laboratory comparisons demonstrated the validity and robustness of hydroxyproline determination according to ISO 17025. Thus, this preliminary identification technique will aid the identification of potentially haram foodstuffs.


Assuntos
Colágeno/análise , Análise de Alimentos/métodos , Gelatina/análise , Hidroxiprolina/análise , Animais , Bovinos , Colágeno/química , Gelatina/química , Suínos
2.
PLoS One ; 15(9): e0239588, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32966345

RESUMO

Despite a growing body of evidence concerning accelerated organic degradation at archaeological sites, there have been few follow-up investigations to examine the status of the remaining archaeological materials in the ground. To address the question of archaeo-organic preservation, we revisited the Swedish, Mesolithic key-site Ageröd and could show that the bone material had been subjected to an accelerated deterioration during the last 75 years, which had destroyed the bones in the areas where they had previously been best preserved. To understand why this has happened and to quantify and qualify the extent of the organic degradation, we here analyse the soil chemistry, bone histology, collagen preservation and palaeobotany at the site. Our results show that the soil at Ageröd is losing, or has already lost, its preservative and buffering qualities, and that pH-values in the still wet areas of the site have dropped to levels where no bone preservation is possible. Our results suggest that this acidification process is enhanced by the release of sulphuric acid as pyrite in the bones oxidizes. While we are still able to find well-preserved palaeobotanical remains, they are also starting to corrode through re-introduced oxygen into the archaeological layers. While some areas of the site have been more protected through redeposited soil on top of the archaeological layers, all areas of Ageröd are rapidly deteriorating. Lastly, while it is still possible to perform molecular analyses on the best-preserved bones from the most protected areas, this opportunity will likely be lost within a few decades. In conclusion, we find that if we, as a society, wish to keep this valuable climatic, environmental and cultural archive, both at Ageröd and elsewhere, the time to act is now and if we wait we will soon be in a situation where this record will be irretrievably lost forever.


Assuntos
Arqueologia/métodos , Fósseis/história , Animais , Osso e Ossos/anatomia & histologia , Osso e Ossos/química , Botânica , Colágeno/análise , Conservação dos Recursos Naturais/métodos , Características Culturais/história , Fósseis/anatomia & histologia , História Antiga , Paleontologia , Plantas/anatomia & histologia , Preservação Biológica/história , Datação Radiométrica , Solo/química , Suécia , Áreas Alagadas
3.
Life Sci ; 261: 118479, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32966840

RESUMO

AIMS: This study was designed to investigate the molecular mechanisms underlying the anti-inflammatory and anti-fibrosis effects of Berberine hydrochloride (BBR) following canalicular laceration (CL) surgical repair. MAIN METHODS: We used a rabbit CL model in this study. BBR and the control medicine were administered during and after the surgical operation. The degree of fibrosis in the canaliculi was evaluated using hematoxylin and eosin and Masson's trichrome staining 7 days after the operation. Inflammation inside the canaliculi was observed using a transcanalicular endoscope. Expression levels of inflammatory cell cytokines [tumor growth factor-ß1 (TGF-ß1), connective tissue growth factor (CTGF), intracellular adhesion molecule-I (ICAM-1), and interleukin-ß1 (IL-1ß)] were detected using immunohistochemistry. P38 and ERK1 phosphorylation and activation were determined using western blot analysis. KEY FINDINGS: The degree of inflammation and fibrosis were less in the BBR groups compared to Surgery group. The anti-inflammatory and anti-fibrosis effects of BBR were concentration-dependent. The levels of TGF-ß1, CTGF, ICAM-1, and IL-1ß were significantly lower in the BBR groups compared to Surgery group. BBR reduced the phosphorylation of P38 compared to Surgery group. SIGNIFICANCE: In conclusion, this study shows that BBR can reduce local fibrosis after CL surgical repair via its anti-inflammatory and anti-fibrosis effects.


Assuntos
Anti-Inflamatórios/uso terapêutico , Berberina/uso terapêutico , Inflamação/tratamento farmacológico , Lacerações/tratamento farmacológico , Animais , Colágeno/análise , Citocinas/análise , Fibrose , Inflamação/patologia , Lacerações/patologia , Masculino , Coelhos
4.
Vet J ; 262: 105515, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32792094

RESUMO

Throughout the ages, humans have selected different horse breeds for their locomotor capacities. Consequently, the properties of equine locomotor tissues could have diversified because of the specific requirements of different disciplines. Therefore, this study aimed to compare biochemical properties of tendons in different equine breeds traditionally selected for racing or sports performance. We hypothesised that tendons in racing breeds would have biochemical properties that would increase strength, whereas those in sporting breeds would have more elastic properties. An ex vivo tendon tissue study comparing the common digital extensor tendon (CDET) and superficial digital flexor tendon (SDFT) of sports horses (Friesian horse, Warmblood horse) and racehorses (Thoroughbred horse; the oldest, reference standard breed) was performed. The SDFT and CDET from middle-aged Friesian (n = 12), Warmblood (n = 12) and Thoroughbred horses (n = 8) were harvested, and their biochemical properties were compared. The biochemical analysis demonstrated significantly higher water percentage, lower collagen concentrations/glycosaminoglycan content and higher crosslink concentrations in the SDFT of sports horses compared to racing breed horses (P < 0.05); DNA content was also significantly lower in sports horses than racehorses (P < 0.05). Racehorses had mainly extra fibrillar collagen support, whereas sports horses had mainly extra crosslink collagen support. From a functional perspective, the racing Thoroughbred relied on stronger tendons, while the sporting Friesians and Warmbloods relied on less stiff, more elastic tendons. In conclusion, there were significant biochemical differences in tendon properties between breeds, possibly related to their intended locomotor performance, although this requires further biomechanical and ultimately genetic confirmation.


Assuntos
Membro Posterior/química , Esportes , Tendões/química , Animais , Colágeno/análise , Glicosaminoglicanos/análise , Cavalos , Seleção Genética
5.
PLoS One ; 15(7): e0235080, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32639959

RESUMO

Detailed information about the lives and deaths of children in antiquity is often in short supply. Childhood dietary histories are, however, recorded and maintained in the teeth of both juveniles and adults. Primary tooth dentinal collagen does not turn over, preserving a sequential record of dietary changes. The use of nitrogen (δ15N) and carbon (δ13C) isotope values of incrementally sampled dentin are used in the study of breastfeeding practices but evidence for the addition of weaning foods, both in terms of mode and, particularly, duration, has remained analytically inaccessible to date. Here, we demonstrate how the novel use hydrogen isotope (δ2H) values of sequentially micro-sampled dentin collagen, measured from individuals excavated from a Punic cemetery, in Sardinia, Italy, can serve as a proxy for weaning food type and duration in ancient childhood diet. The weaning rate and age, based on the decline in δ15N and δ13C values of permanent first molars and the concomitant increase in δ2H, appears to be broadly similar among six individuals. Hydrogen isotopes vary systematically from a low value soon after birth, rising through early childhood. The early post-birth values can be explained by the influence of 2H-depleted lipids from mother's breastmilk and the later δ2H rise is consistent with, among other things, a substantial portion of boiled foodstuffs, such as the higher δ2H values observed in porridge. Overall δ2H in dentin shows great promise to elucidate infant and childhood feeding practices, and especially the introduction of supplementary foods during the weaning process.


Assuntos
Dieta/história , Aleitamento Materno/história , Pré-Escolar , Colágeno/análise , Dentina/química , Deutério/análise , Feminino , História Antiga , Humanos , Lactente , Itália , Desmame
6.
Int. j. morphol ; 38(3): 755-760, June 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1098316

RESUMO

SUMMARY: The objective of this study was to describe the effects of monosodium glutamate on the collagen of the parotid gland in an obesity model. 18 newborn male Sprague Dawley rats were used (first control group; second group of MSG1: 4 mg/g of monosodium glutamate weight, 5 doses, and third group of MSG2: 4 mg/g of monosodium glutamate, 5 doses, maintained for 8 and 16 weeks respectively). The content and type of collagen were analyzed, in addition to the levels of cholesterol, glucose, triglycerides and uric acid. Monosodium glutamate produced an increase in the obesity rates of the MSG2 group, in addition to an increase in blood cholesterol, glucose and uric acid levels compared to the control group. Type III collagen in the MSG2 group showed a statistically significant increase. Monosodium glutamate induced obesity, in addition to an increase in type III collagen fibers.


RESUMEN: El objetivo de este estudio fue describir los efectos del glutamato monosódico sobre el colágeno de la glándula parótida en un modelo de obesidad. Se utilizaron 18 ratas Sprague Dawley machos recién nacidas (primer grupo control; segundo grupo MSG1: 4 mg/g de peso de glutamato monosódico, 5 dosis, y tercer grupo MSG2: 4 mg/g de glutamato monosódico, 5 dosis, mantenidas durante 8 y 16 semanas respectivamente). Se analizó el contenido y el tipo de colágeno, además de los niveles de colesterol, glucosa, triglicéridos y ácido úrico. El glutamato monosódico produjo un aumento en las tasas de obesidad del grupo MSG2, además de un aumento en los niveles de colesterol en sangre, glucosa y ácido úrico en comparación con el grupo control. El colágeno tipo III en el grupo MSG2 mostró un aumento estadísticamente significativo. La obesidad inducida por glutamato monosódico, además de un aumento en las fibras de colágeno tipo III.


Assuntos
Animais , Masculino , Ratos , Glândula Parótida , Glutamato de Sódio/toxicidade , Colágeno/efeitos dos fármacos , Obesidade/induzido quimicamente , Glândulas Salivares/efeitos dos fármacos , Triglicerídeos/sangue , Ácido Úrico/sangue , Glicemia/análise , Peso Corporal/efeitos dos fármacos , Colesterol/sangue , Colágeno/análise , Ratos Sprague-Dawley , Modelos Animais de Doenças , Animais Recém-Nascidos
7.
Cardiovasc Pathol ; 48: 107224, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32480283

RESUMO

OBJECTIVE: We used automated image analysis software to determine the proportion of collagen, fat, and myocytes across six histological regions of normal ventricular myocardium. METHODS: Twenty-nine non-cardiac death cases referred to our national cardiac pathology center were included in this study. Whole hearts were macroscopically and microscopically normal following expert histopathological evaluation. Tissue sections from the right ventricular outflow tract, right ventricle (RV), anterior interventricular septum (IVS), posterior IVS, anterior left ventricle (LV), and posterior LV were stained with Picrosirius red for collagen and scanned using a high-resolution slide scanner. Quantification of collagen, fat, and myocyte proportions was performed using Visiopharm software after automated exclusion of perivascular collagen. RESULTS: The majority of decedents were male (25/29; 86%) with a mean age at death of 32.1 ± 9.9 (range 18-54) and mean BMI 28.7 ± 7.3. We report predicted values (collagen %, fat %, myocytes %) for cardiac tissue composition within the RV, IVS, and LV (including epicardial and endocardial layers). The proportion of collagen and fat were higher in the RV compared with the LV (ratios 1.61 [1.45-1.78]; 2.63 [1.99-3.48], respectively) and RV compared with the IVS (ratios 1.77 [1.60-1.97]; 8.41[6.35-11.13], respectively). The ratio of epicardial versus endocardial fat was increased in both ventricles (RV: ratio 4.49 [3.67-5.49]; LV: ratio 3.46 [2.49-4.81]). In multivariable analysis, there was no significant association between collagen or fat proportion and sex (p=0.12; p=0.08, respectively), age at death (p=0.36; p=0.23, respectively), or BMI (p=0.45; p=0.43, respectively). CONCLUSIONS: Our findings provide location and sex-specific proportions of myocardial histological tissue composition that may aid quantitative evaluation of pathology in future studies.


Assuntos
Tecido Adiposo/patologia , Colágeno/análise , Ventrículos do Coração/química , Ventrículos do Coração/patologia , Miócitos Cardíacos/química , Miócitos Cardíacos/patologia , Adolescente , Adulto , Autopsia , Feminino , Fibrose , Humanos , Masculino , Pessoa de Meia-Idade , Fatores Sexuais , Adulto Jovem
8.
J Zoo Wildl Med ; 51(2): 280-289, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32549556

RESUMO

This study aimed to evaluate the ophthalmic parameters, morphometric features of corneal tissue, and arrangements of corneal stromal collagen fibers in crab-eating fox (Cerdocyon thous), a species of neotropical wild canid. We conducted the study on six juvenile crab-eating foxes (12 eyes), whilst 16 eyes were obtained post mortem from eight adult crab-eating foxes. The research was divided into two stages. In the first stage, eye anatomical characteristics, tear production (Schirmer 1 tear test, STT1), intraocular pressure (IOP), ocular echobiometry, and specular microscopy parameters related to morphology of corneal endothelium were studied in juvenile animals. In the second stage, morphometric features of corneal tissue (central corneal thickness [CCT] and corneal epithelium thickness) and arrangements of stromal collagen fibers were studied using eyes from adult animals. The main findings were that crab-eating fox eyes have vertical-slit pupils, holangiotic retina, and reference values (mean ± SD) of 13.37 ± 3.79 mm/min for STT1 and of 10.43 ± 3.84 mmHg for IOP. The ocular echobiometric features observed in crab-eating foxes are different from those reported for domestic dogs (Canis familiaris). Conversely, the corneal endothelial parameters are similar to those of domestic dogs. The CCT measured by tissue morphometry was 0.54 ± 0.06 mm, and the corneal epithelium thickness was 60.13 ± 8.71 µm. Mean coherency related to alignment of collagen fibers was 0.66 ± 0.12. The crab-eating fox cornea had predominantly thick collagen fibers. Crab-eating fox eyes have morphofunctional peculiarities. They resemble the eyes of domestic dogs in some aspects, but diverge in others.


Assuntos
Canidae/anatomia & histologia , Colágeno/análise , Córnea/anatomia & histologia , Animais , Brasil , Testes Diagnósticos de Rotina/veterinária , Valores de Referência
9.
J Morphol ; 281(6): 676-687, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32369248

RESUMO

In riverine ecosystems, downstream drag caused by fast-flowing water poses a significant challenge to rheophilic organisms. In neotropical rivers, many members of a diverse radiation of suckermouth catfishes (Loricarioidei) resist drag in part by using modified lips that form an oral suction cup composed of thick flesh. Histological composition and morphology of this cup are interspecifically highly variable. Through an examination of 23 loricarioid species, we determined that the tissue most responsible for lip fleshiness is collagen. We hypothesized that lip collagen content is interspecifically correlated with substrate and flow so that fishes living on rocky substrates in high-flow environments have the largest, most collagenous lips. By mapping the amount and distribution of lip collagen onto a phylogeny and conducting ANOVA tests, we found support for this hypothesis. Moreover, these traits evolved multiple times in correlation with substrate and flow, suggesting they are an effective means for improving suction-based attachment. We hypothesize that collagen functions to reinforce oral suction cups, reducing the likelihood of slipping, buckling, and failure under high-flow, high-drag conditions. Macroevolutionary patterns among loricarioid catfishes suggest that for maximum performance, biomimetic suction cups should vary in material density according to drag and substrate requirements.


Assuntos
Evolução Biológica , Peixes-Gato/anatomia & histologia , Colágeno/análise , Lábio/química , Boca/anatomia & histologia , Reologia , Animais , Análise dos Mínimos Quadrados , Fenótipo , Filogenia , Análise de Componente Principal , Sucção
10.
Int J Cardiovasc Imaging ; 36(9): 1761-1769, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32409978

RESUMO

Right ventricular biopsy represents the gold standard for the assessment of myocardial fibrosis and collagen content. This invasive technique, however, is accompanied by perioperative complications and poor reproducibility. Extracellular volume (ECV) measured through cardiovascular magnetic resonance (CMR) has emerged as a valid surrogate method to assess fibrosis non-invasively. Nonetheless, ECV provides an overestimation of collagen concentration since it also considers interstitial space. Our study aims to investigate the feasibility of estimating total collagen volume (TCV) through CMR by comparing it with the TCV measured at histology. Seven healthy Landrace pigs were acutely instrumented closed-chest and transported to the MRI facility for measurements. For each protocol, CMR imaging at 3T was acquired. MEDIS software was used to analyze T1 mapping and ECV for both the left ventricular myocardium (LVmyo) and left ventricular septum (LVseptum). ECV was then used to estimate TCVCMR at LVmyo and LVseptum following previously published formulas. Tissues were prepared following an established protocol and stained with picrosirius red to analyze the TCVhisto in LVmyo and LVseptum. TCV measured at LVmyo and LVseptum with both histology (8 ± 5 ml and 7 ± 3 ml, respectively) and T1-Mapping (9 ± 5 ml and 8 ± 6 ml, respectively) did not show any regional differences. TCVhisto and TCVCMR showed a good level of data agreement by Bland-Altman analysis. Estimation of TCV through CMR may be a promising way to non-invasively assess myocardial collagen content and may be useful to track disease progression or treatment response.


Assuntos
Colágeno/análise , Cardiopatias/diagnóstico por imagem , Coração/diagnóstico por imagem , Imagem Cinética por Ressonância Magnética , Miocárdio/química , Animais , Biópsia , Estudos de Viabilidade , Fibrose , Cardiopatias/metabolismo , Cardiopatias/patologia , Miocárdio/patologia , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Sus scrofa
11.
J Food Sci ; 85(6): 1675-1680, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32469102

RESUMO

Collagen-based sauce braised meat products with a unique flavor, chewiness, and springiness are very popular in China. In order to understand how the structure, thermal properties, and chemical components of collagen-based tissues influence the texture characteristics of collagen-based meat products during stewing, three kinds of tendon tissues (chicken feet tendon [CFT], sheep tendon [ST], and bovine tendon [BT]) and three kinds of skin tissues (chicken feet skin [CFS], sheep skin [SS], and porcine skin [PS]) were selected to heat for 45 min at 95 °C, and texture, thermal properties, micromorphology, ultrastructure, glycosaminoglycan (GAG) content, and collagen content were evaluated among the six kinds of collagen-based tissues. Results showed that BT had the highest hardness, chewiness values, total collagen content, and the thickest fibers diameters, followed by ST, CFT, PS, and SS, whereas CFS had lowest values among six kinds of tissues. The thermal stability and GAG levels in tendon samples were higher than those in skin samples. Thicker diameters, higher thermal stability, GAG, and total collagen content could be responsible for the higher hardness and chewiness of tendon samples compared with skin tissues. These findings provide the understanding of relationship between meat quality of collagen-based meat products and their morphological and chemical properties. PRACTICAL APPLICATION: This research confirms that the difference in texture of different kinds of collagen-based tissues was related to the difference in structure and chemical properties. The result can be used to optimize the processing conditions of collagen-based sauce braised meat products in food industry.


Assuntos
Colágeno/análise , Produtos da Carne/análise , Animais , Bovinos , Galinhas , China , Culinária , Indústria de Processamento de Alimentos , Dureza , Ovinos , Suínos , Paladar
12.
Poult Sci ; 99(4): 2033-2040, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32241488

RESUMO

Many requirements are necessary to meet the European Union rules to export poultry, including the amount of physiological water and water-protein ratio (WPR) in carcasses. Therefore, the aim of this study was to identify if strain, nutrition, and age affect the amount of collagen and fat and the WPR in cuts and verify whether the latter meets the international export standards. A total of 3,240 male chicks were housed in a completely randomized design in a 3 × 3 × 5 factorial arrangement, which included 3 nutritional densities (regular, medium, and high), 3 strains (021 Embrapa and 2 commercial strains identified as A and B), and 5 ages. Twelve broilers from each treatment (totaling 540 birds) were slaughtered at 28, 35, 42, 49, and 56 D of age to determine collagen and fat levels and WPR (through the calculation of moisture and protein percentage) in broiler breasts and legs using the near-infrared spectroscopy method. The use of feeds with different nutritional densities presented in this study has no effect on the WPR in the breast and legs of broilers slaughtered between 28 and 56 D of age. However, nutritional density influences liveweight and percentage of fat in the breast and legs. Collagen percentage in the legs decreases with increasing nutritional density. The 021 Embrapa strain cuts present a lower WPR than those of other commercial strains. However, the values found for all strains studied are within the limits of the Europe Union and Brazilian legislations. The liveweight, breast weight, leg weight, and leg fat increases linearly with age. Quite the opposite, water protein ratio, breast fat level, and breast collagen level decrease linearly with age. Leg WPR and leg collagen level are not affected by age. Despite the differences found for strains, nutritional densities and age readers should be aware that these factors may interact with each other depending on the response variable studied.


Assuntos
Galinhas/fisiologia , Dieta/veterinária , Membro Posterior/química , Carne/análise , Estado Nutricional , Músculos Peitorais/química , Fatores Etários , Ração Animal/análise , Animais , Proteínas Aviárias/análise , Brasil , Galinhas/genética , Colágeno/análise , Lipídeos/análise , Masculino , Água/análise
13.
Einstein (Sao Paulo) ; 18: eAO5105, 2020.
Artigo em Inglês, Português | MEDLINE | ID: mdl-32159607

RESUMO

OBJECTIVE: To evaluate the density of anti-galectin-3-immunostained cells, collagen percentage, mast cell density and presence of pathological processes in intestinal muscle biopsies of patients. METHODS: Thirty-five patients who underwent intestinal biopsy were selected from 1997 to 2015. Patients were divided into three groups: chagasic patients with mucosal lesion (n=13), chagasic patients with intact mucosa (n=12) and non-chagasic patients with no mucosal lesion (n=10). Histological processing of the biopsied fragments and immunohistochemistry for galectin-3 were performed. Additional sections were stained with hematoxylin and eosin to evaluate the general pathological processes, picrosirius for evaluation of collagen and toluidine blue to evaluate the mast cell density. RESULTS: Patients of mucosal lesion group had a significantly higher frequency of ganglionitis and myositis when compared to the chagasic patients with intact mucosa and non-chagasic group. The density of anti-galectin-3-immunostained cells was significantly higher in the chagasic patients with intact mucosa group when compared to the non-chagasic group. The group of chagasic patients with intact mucosa presented a higher percentage of collagen in relation to the patients with mucosal lesion and to the non-chagasic group, with a significant difference. There was no significant difference in mast cell density among the three groups. CONCLUSION: The higher density of anti-galectin-3-immunostained cells in patients in the chagasic patients with intact mucosa group suggested the need for greater attention in clinical evaluation of these patients, since this protein is associated with neoplastic transformation and progression.


Assuntos
Anticorpos Monoclonais/análise , Doença de Chagas/patologia , Colonoscopia/métodos , Galectina 3/análise , Mucosa Intestinal/patologia , Megacolo/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Análise de Variância , Biópsia , Estudos de Casos e Controles , Contagem de Células , Colágeno/análise , Feminino , Fibrose , Galectina 3/imunologia , Humanos , Imuno-Histoquímica , Masculino , Mastócitos/patologia , Pessoa de Meia-Idade , Miosite/patologia , Estudos Retrospectivos , Estatísticas não Paramétricas
14.
PLoS One ; 15(3): e0230657, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32208438

RESUMO

BACKGROUND: High-grade inflammation may play a pivotal role in the pathogenesis of left ventricular (LV) dysfunction. Evidence to support a role of systemic inflammation in mediating impaired LV function in experimental models of rheumatoid arthritis (RA) remains limited. The aim of the present study was to determine the effects of high-grade systemic inflammation on LV diastolic and systolic function in collagen-induced arthritis (CIA). METHODS: To induce CIA, bovine type-II collagen emulsified in incomplete Freund's adjuvant was injected at the base of the tail into 21 three-month old Sprague Dawley rats. Nine-weeks after the first immunisation, LV function was assessed by pulsed Doppler, tissue Doppler imaging and Speckle tracking echocardiography. Cardiac collagen content was determined by picrosirius red staining; circulating inflammatory markers were measured using ELISA. RESULTS: Compared to controls (n = 12), CIA rats had reduced myocardial relaxation as indexed by lateral e' (early diastolic mitral annular velocity) and e'/a' (early-to-late diastolic mitral annular velocity) and increased filling pressures as indexed by E/e'. No differences in ejection fraction and LV endocardial fractional shortening between the groups were recorded. LV global radial and circumferential strain and strain rate were reduced in CIA rats compared to controls. Higher concentrations of circulating inflammatory markers were associated with reduced lateral e', e'/a', radial and circumferential strain and strain rate. Greater collagen content was associated with increased concentrations of circulating inflammatory markers and E/e'. CONCLUSION: High-grade inflammation is associated with impaired LV diastolic function and greater myocardial deformation independent of haemodynamic load in CIA rats.


Assuntos
Artrite Experimental/fisiopatologia , Função Ventricular Esquerda/fisiologia , Animais , Artrite Experimental/induzido quimicamente , Biomarcadores/sangue , Biomarcadores/metabolismo , Pressão Sanguínea , Peso Corporal , Proteína C-Reativa/análise , Proteína C-Reativa/metabolismo , Bovinos , Colágeno/análise , Colágeno Tipo II/toxicidade , Ecocardiografia Doppler de Pulso , Interleucina-1beta/sangue , Interleucina-1beta/metabolismo , Masculino , Miocárdio/metabolismo , Miocárdio/patologia , Ratos , Ratos Sprague-Dawley , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/metabolismo
15.
Pathol Res Pract ; 216(3): 152831, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32005407

RESUMO

Collagen (COL) genes participate in tumor extracellular matrix (ECM)-receptor interactions and focal adhesion pathways, which play a crucial role in tumor invasion and metastasis. The prognostic value of COL genes has been shown for several malignancies. In the present study, we analyzed multiple microarray datasets using the Oncomine database to identify alterations of COL genes in gastric cancer (GC). Gene expression levels were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) in GC tissues and matched adjacent tissues. The prognostic value of differentially expressed COL genes in GC was evaluated by Kaplan-Meier survival analysis based on the complete mRNA transcriptomics data from The Cancer Genome Atlas (TCGA). We found that seven COL genes (COL1A2, COL4A1, COL4A2, COL6A1, COL6A2, COL6A3, and COL11A1) were elevated in GC. Among them, stepwise multivariate Cox regression was applied, and it was determined that COL4A1 and COL4A2 were signature and independent prognostic biomarkers in GC patients with obviously different overall survival (OS). High expression of COL4A1, COL4A2, COL6A1, COL6A2, and COL6A3 was correlated with poorer prognosis of GC patients treated by surgery only, while higher expression of COL4A1 and COL11A1 correlated with poorer survival of patients treated by 5-fluorouracil-based adjuvant therapy. Our results indicate that overexpression of COL genes might be utilized as novel prognostic markers for GC and assist with therapy selection.


Assuntos
Biomarcadores Tumorais/análise , Colágeno/biossíntese , Neoplasias Gástricas/patologia , Transcriptoma , Adulto , Idoso , Antineoplásicos/uso terapêutico , Quimioterapia Adjuvante/métodos , Quimioterapia Adjuvante/mortalidade , Colágeno/análise , Feminino , Fluoruracila/uso terapêutico , Gastrectomia/métodos , Gastrectomia/mortalidade , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Neoplasias Gástricas/terapia
16.
Biochem Biophys Res Commun ; 524(4): 943-950, 2020 04 16.
Artigo em Inglês | MEDLINE | ID: mdl-32059850

RESUMO

Wheat germ derivatives have been shown to inhibit inflammation-related diseases. In this study, a small peptide (YDWPGGRN) isolated from wheat germ was used to study its anti-inflammatory activity and its application in skin wound healing. Both the in vitro and in vivo results clearly showed that YDWPGGRN significantly inhibited the LPS-stimulated NO, IL-1ß, IL-6 and TNF-α production but promoted the release of an anti-inflammatory cytokine, IL-10. In addition, YDWPGGRN directly enhanced the proliferation and migration of HaCaT cells and L929 cells. Furthermore, the results demonstrated that YDWPGGRN was able to stimulate angiogenesis and collagen production in wound areas, consequently accelerating the skin wound-healing processes in a rat model with a full thickness dermal wound. The current findings suggest that YDWPGGRN promotes wound healing by anti-inflammatory reactions and enhances the proliferation and migration of keratinocytes and fibroblasts; therefore, it may be applicable for skin wound therapeutics.


Assuntos
Anti-Inflamatórios/uso terapêutico , Peptídeos/uso terapêutico , Pele/efeitos dos fármacos , Triticum , Cicatrização/efeitos dos fármacos , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Colágeno/análise , Humanos , Inflamação/tratamento farmacológico , Inflamação/patologia , Masculino , Camundongos , Peptídeos/química , Peptídeos/farmacologia , Células RAW 264.7 , Ratos Sprague-Dawley , Pele/patologia , Triticum/química
17.
J Sci Food Agric ; 100(6): 2502-2511, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31960978

RESUMO

BACKGROUND: The present study aimed to identify relationships between components of intramuscular connective tissue, proportions of the different fiber types, intramuscular fat and sensory tenderness of beef cooked at 55 °C. Accordingly, four muscles differing in their metabolic and contractile properties, as well as in their collagen content and butcher value, were obtained from dairy and beef cattle of several ages and sexes and were then used to create variability. RESULTS: Correlation analyses and/or stepwise regressions were applied on Z-scores to identify the existing and robust associations. Tenderness scores were further categorized into tender, medium and tough classes using unsupervised learning methods. The findings revealed a muscle-dependant role with respect to tenderness of total and insoluble collagen, cross-links, and type IIB + X and IIA muscle fibers. The longissimus thoracis and semitendinosus muscles that, in the present study, were found to be extreme in their tenderness potential were also very different from each other and from the rectus abdominis (RA) and semimembranosus (SM). RA and SM muscles were very similar regarding their relationship for muscle components and tenderness. A relationship between marbling and tenderness was only present when the results were analysed irrespective of all factors of variation of the experimental model relating to muscle and animal type. CONCLUSION: The statistical approaches applied in the present study using Z-scores allowed identification of the robust associations between muscle components and sensory beef tenderness and also identified discriminatory variables of beef tenderness classes. © 2020 Society of Chemical Industry.


Assuntos
Tecido Conjuntivo , Fibras Musculares Esqueléticas , Carne Vermelha/análise , Tecido Adiposo , Animais , Bovinos/classificação , Colágeno/análise , Culinária , Feminino , Humanos , Masculino , Músculo Esquelético , Resistência ao Cisalhamento
18.
Sci Rep ; 10(1): 1125, 2020 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-31980663

RESUMO

Arising from the ablation of the cytoskeletal protein dystrophin, Duchenne Muscular Dystrophy (DMD) is a debilitating and fatal skeletal muscle wasting disease underpinned by metabolic insufficiency. The inability to facilitate adequate energy production may impede calcium (Ca2+) buffering within, and the regenerative capacity of, dystrophic muscle. Therefore, increasing the metabogenic potential could represent an effective treatment avenue. The aim of our study was to determine the efficacy of adenylosuccinic acid (ASA), a purine nucleotide cycle metabolite, to stimulate metabolism and buffer skeletal muscle damage in the mdx mouse model of DMD. Dystrophin-positive control (C57BL/10) and dystrophin-deficient mdx mice were treated with ASA (3000 µg.mL-1) in drinking water. Following the 8-week treatment period, metabolism, mitochondrial density, viability and superoxide (O2-) production, as well as skeletal muscle histopathology, were assessed. ASA treatment significantly improved the histopathological features of murine DMD by reducing damage area, the number of centronucleated fibres, lipid accumulation, connective tissue infiltration and Ca2+ content of mdx tibialis anterior. These effects were independent of upregulated utrophin expression in the tibialis anterior. ASA treatment also increased mitochondrial viability in mdx flexor digitorum brevis fibres and concomitantly reduced O2- production, an effect that was also observed in cultured immortalised human DMD myoblasts. Our data indicates that ASA has a protective effect on mdx skeletal muscles.


Assuntos
Monofosfato de Adenosina/análogos & derivados , Distrofia Muscular Animal/tratamento farmacológico , Monofosfato de Adenosina/uso terapêutico , Animais , Cálcio/análise , Linhagem Celular Transformada , Colágeno/análise , Avaliação Pré-Clínica de Medicamentos , Transporte de Elétrons/efeitos dos fármacos , Humanos , Lipídeos/análise , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos mdx , Mitocôndrias Musculares/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Músculo Esquelético/patologia , Distrofia Muscular Animal/patologia , Distrofia Muscular de Duchenne/patologia , Mioblastos/metabolismo , Biogênese de Organelas , Consumo de Oxigênio/efeitos dos fármacos , Superóxidos/metabolismo , Utrofina/biossíntese , Utrofina/genética
19.
Int J Radiat Biol ; 96(4): 448-460, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-31985334

RESUMO

Purpose: The article reports on a comparative analysis of biological specimens of lung tissues collected from workers with pulmonary fibrosis induced by internal exposure to plutonium alpha-particles (plutonium-induced pulmonary fibrosis [PuPF]) and with etiologically different pulmonary fibrosis (non-PuPF) that developed as an outcome of a chronic obstructive pulmonary disease (COPD).Materials and methods: To perform histological examinations, lung tissues were sampled during autopsy. Six samples of various lung regions (the apical region, the lingula of the left lung and the inferior lobe) were collected from each donor. The resected tissue samples were fixed in 10% neutral-buffered formalin during 24 h and embedded into paraffin blocks (FFPE). FFPE blocks with lung tissue specimens collected from 56 workers with PuPF, 34 workers with non-PuPF and 35 workers without any lung disease were used in the study. To perform microscopic examination, lung tissue specimens were hematoxylin and eosin stained. To examine the connective-tissue scaffold of lung stroma and identify foci of pulmonary fibrosis, the cut sections of paraffin blocks were stained by Van Gizon's method (to assess the total volume of fibrosis-affected tissues), Gomori's technique (to define the reticular scaffold of lung stroma) and Weigert's technique (to examine elastic fibers). Morphological patterns of all biological specimens were studied using immunohistochemistry. To fit the empirical data, the Weibull's model was used.Results and conclusions: The study found qualitative and quantitative morphological features specific for PuPF compared to non-PuPF. The study demonstrated that hyper-production of collagen type V plays a key role in PuPF. The collagen type V content in fibrotic foci in lung tissue specimens from workers with PuPF was found to be increased.


Assuntos
Partículas alfa/efeitos adversos , Doenças Profissionais/patologia , Fibrose Pulmonar/patologia , Lesões por Radiação/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Colágeno/análise , Colágeno/biossíntese , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/etiologia , Doenças Profissionais/metabolismo , Fibrose Pulmonar/etiologia , Fibrose Pulmonar/metabolismo , Fator de Crescimento Transformador beta1/fisiologia
20.
Meat Sci ; 160: 107963, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31693966

RESUMO

Quality of bovine longissimus lumborum, infraspinatus and cleidooccipitalis muscles after high-intensity ultrasound (HIU; 40 kHz and a power of 11 W/cm2 for 0, 40, 60, and 80 min) and aging (0, 7 and 14 d) was evaluated. The effects of HIU on pH and color of meat were not considered negative. HIU improved water holding capacity (WHC) of l.lumborum and infraspinatus only after aging. Whereas, the WHC of cleidooccipitalis increased immediately after sonication. The total collagen of HIU treated samples was significantly lower compared to the untreated samples. Ultrasonication for 80 min was the most effective for infraspinatus and cleidooccipitalis. Toughness decreased with HIU, iii nfraspinatus and l.lumborum tenderized more than cleidooccipitalis. HIU application and 7 d aging is an excellent combined treatment to improve tenderness of the three muscles. Infraspinatus was the most tender meat. HIU could help industry to improve the quality of beef as it helps in tenderization and accelerates maduration particularly of l.lumborum.


Assuntos
Manipulação de Alimentos/métodos , Carne Vermelha/análise , Ondas Ultrassônicas , Animais , Bovinos , Colágeno/análise , Cor , Concentração de Íons de Hidrogênio , Músculo Esquelético/química , Resistência ao Cisalhamento , Água
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