Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 8.438
Filtrar
1.
Einstein (Sao Paulo) ; 18: eAO4876, 2020.
Artigo em Inglês, Português | MEDLINE | ID: mdl-31576909

RESUMO

OBJECTIVE: To investigate the effects of sericin extracted from silkworm Bombyx mori cocoon on morphophysiological parameters in mice with obesity induced by high-fat diet. METHODS: Male C57Bl6 mice aged 9 weeks were allocated to one of two groups - Control and Obese, and fed a standard or high-fat diet for 10 weeks, respectively. Mice were then further subdivided into four groups with seven mice each, as follows: Control, Control-Sericin, Obese, and Obese-Sericin. The standard or high fat diet was given for 4 more weeks; sericin (1,000mg/kg body weight) was given orally to mice in the Control-Sericin and Obese-Sericin Groups during this period. Weight gain, food intake, fecal weight, fecal lipid content, gut motility and glucose tolerance were monitored. At the end of experimental period, plasma was collected for biochemical analysis. Samples of white adipose tissue, liver and jejunum were collected and processed for light microscopy analysis; liver fragments were used for lipid content determination. RESULTS: Obese mice experienced significantly greater weight gain and fat accumulation and had higher total cholesterol and glucose levels compared to controls. Retroperitoneal and periepididymal adipocyte hypertrophy, development of hepatic steatosis, increased cholesterol and triglyceride levels and morphometric changes in the jejunal wall were observed. CONCLUSION: Physiological changes induced by obesity were not fully reverted by sericin; however, sericin treatment restored jejunal morphometry and increased lipid excretion in feces in obese mice, suggesting potential anti-obesity effects.


Assuntos
Fármacos Antiobesidade/uso terapêutico , Dieta Hiperlipídica , Obesidade/tratamento farmacológico , Sericinas/uso terapêutico , Tecido Adiposo/patologia , Animais , Fármacos Antiobesidade/farmacologia , Peso Corporal/efeitos dos fármacos , Colesterol/análise , Dieta Hiperlipídica/efeitos adversos , Ingestão de Alimentos/efeitos dos fármacos , Fígado Gorduroso/patologia , Trânsito Gastrointestinal/efeitos dos fármacos , Teste de Tolerância a Glucose , Fígado/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Camundongos Obesos , Obesidade/etiologia , Obesidade/fisiopatologia , Reprodutibilidade dos Testes , Sericinas/farmacologia , Fatores de Tempo , Resultado do Tratamento , Triglicerídeos/análise , Ganho de Peso/efeitos dos fármacos
2.
Food Chem ; 301: 125198, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31374533

RESUMO

The experiment was conducted to evaluate the effects of five rubber seed oil (RSO) levels (0, 1%, 2%, 4%, and 6%) on hens laying performance, egg quality, and yolks fatty acid composition and cholesterol contents. Three hundred and sixty 30-week-old Lohmann Brown laying hens were allotted to 5 groups. The results showed that the egg production was increased in 4% RSO group (P < 0.05), but egg quality parameters and the contents of dry matter, lipid, and protein in yolks were not influenced among treatments (P > 0.05). Yolk cholesterol contents were reduced in RSO supplemental groups (P < 0.05). The concentration of total n-3 PUFA in yolks increased gradually while the ratio of n-6/n-3 decreased gradually with increasing dietary RSO levels (P < 0.001). In conclusion, dietary RSO supplementation increased yolk n-3 PUFA levels, improved yolk color, and reduced yolk cholesterol contents without negative influence on laying performance parameters.


Assuntos
Ração Animal , Galinhas , Colesterol/metabolismo , Gema de Ovo/química , Gorduras Insaturadas/farmacologia , Ácidos Graxos Ômega-3/metabolismo , Animais , Galinhas/metabolismo , Colesterol/análise , Suplementos Nutricionais , Gema de Ovo/metabolismo , Ácidos Graxos Ômega-3/análise , Feminino , Qualidade dos Alimentos , Armazenamento de Alimentos , Lipídeos/análise
3.
Food Chem ; 298: 125088, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260987

RESUMO

Infant formula certified reference material (CRM, KRISS CRM 108-02-003) were developed for the analysis of organic nutrients. The CRM is a milk-based infant formula powder, packaged at 14 g per unit. Ten thousand units were prepared and stored at -70 °C. For the certification of each nutrient, ten units were analyzed for simultaneous value-assignment and homogeneity test. Analytical methods used were isotope dilution mass spectrometry (IDMS) based on liquid chromatography mass spectrometer (LC/MS) or gas chromatography mass spectrometer (GC/MS) as higher-order reference methods.13 vitamins, 3 fatty acids, and total cholesterol were certified. The between-unit relative standard deviation of measurement results for each nutrient ranged 0.2% to 2.5%, showing very good homogeneity. The expanded relative uncertainties of the certified values ranged from 1% to 8%, indicating that they have higher-order metrological quality. The values of proximates (proteins, lipids, carbohydrates, water, and ash) were assigned through inter-laboratory comparisons.


Assuntos
Análise de Alimentos/métodos , Fórmulas Infantis/análise , Fórmulas Infantis/normas , Certificação , Colesterol/análise , Cromatografia Líquida/métodos , Ácidos Graxos/análise , Análise de Alimentos/normas , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Lactente , Nutrientes/análise , Padrões de Referência , Vitaminas/análise
4.
Nat Protoc ; 14(8): 2546-2570, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31341291

RESUMO

Distal cholesterol biosynthesis (CB) has recently taken center stage as a promising drug target in several diseases previously not linked to this biochemical pathway, including cardiovascular disease, cancer, multiple sclerosis and Alzheimer's disease. Most enzymes involved in this pathway are hard to isolate, warranting dedicated analytical tools for biochemical screening. We describe the use of gas chromatography-electron ionization mass spectrometry (GC-MS) in a whole-cell screening assay aimed at monitoring interactions with all enzymes of distal CB in a single experiment. Following cell culture and lipid extraction, the trimethylsilyl ethers of sterols are analyzed by GC-MS. Analytical data for 23 relevant sterols (intermediates) are provided, allowing their unambiguous identification. Sterol pattern analysis reveals the target enzyme on the basis of characteristic marker sterols, whereas quantification of 2-13C-acetate incorporation correlates with the inhibitory activity of drug candidates. The protocol can be used by both experienced scientists and newcomers to the field, allowing detection and quantification of small molecule-enzyme interactions in distal CB. The entire protocol can be carried out within two working days.


Assuntos
Colesterol/metabolismo , Técnicas Citológicas/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Bioensaio/métodos , Colesterol/análise , Colesterol/química , Sistemas de Liberação de Medicamentos , Células HL-60 , Humanos , Esteróis/análise , Esteróis/química , Esteróis/metabolismo
5.
J Dairy Sci ; 102(9): 7723-7733, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31255261

RESUMO

Milk fat adulteration is a common issue in Central Asia. To assess the current situation in the commercial milk market, 17 milk samples were checked for fatty acid (FA) and sterol profiles to detect potential adulteration using multivariate analysis. Analysis of FA and sterols was performed using gas chromatography with flame ionization detection and gas chromatography with mass-spectrometric detection, respectively. Cluster analysis of FA profiles revealed 3 types of milk samples: (1) samples containing a higher proportion of short-chain FA, (2) samples containing a higher proportion of long-chain FA, and (3) samples with significant amounts of C18 FA. Analysis of sterols showed that samples included (1) milk fat containing 100% cholesterol, sometimes with traces of phytosterols, (2) milk fat with high proportions of ß-sitosterol and campesterol, and (3) milk fat containing high proportions of brassicasterol. We found significant relationships between FA profiles and sterol profiles. The profiles were compared with vegetable oil patterns reported in the literature. More than 50% of the samples appeared to be counterfeited. We conclude that identification of adulteration in milk can be based solely on determination of sterol patterns.


Assuntos
Ácidos Graxos/análise , Contaminação de Alimentos/análise , Leite/química , Esteróis/análise , Animais , Colestadienóis/análise , Colesterol/análogos & derivados , Colesterol/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Fitosteróis/análise , Óleos Vegetais/análise , Sitosteroides/análise
6.
J Sci Food Agric ; 99(13): 5890-5898, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31206714

RESUMO

BACKGROUND: Eggs are important foods in the daily diet of humans and have great biological activity and a high digestibility. Egg yolk is a good source of biologically active substances such as fatty acids, phospholipids, sterols and tocopherols. The eggs of seven chicken genotypes were analyzed for their chemical composition, and a detailed study of the lipids in egg yolk was conducted. RESULTS: Energy composition of the egg yolk and egg albumen was 29.06-30.51 MJ kg-1 and 19.77-20.93 MJ kg-1 respectively. Regarding their chemical composition: water ranged from 471.7 to 515.4 g kg-1 and 878.3-885.9 g kg-1 ; fat content in dry matter ranged from 607 to 647 g kg-1 and 6.7-11.6 g kg-1 ; protein varied from 302 to 331.7 g kg-1 and 823.6-892.5 g kg-1 ; ash ranged from 33.7 to 37.7 g kg-1 and 63.8-74.0 g kg-1 ; and nitrogen-free extracts ranged from 12.7 to 36.5 g kg-1 and 35.0-96.2 g kg-1 . The sterols and phospholipids in the yolk lipids were 16-26 g kg-1 and 59-127 g kg-1 . The main fatty acids in the lipids were oleic (39.1-47.3%) and palmitic (26.0-35.5%) acids. Cholesterol in the yolk lipids ranged from 15.9 to 25.9 g kg-1 . Phosphatidylcholine (389-573 g kg-1 ), phosphatidylethanolamine (219-355 g kg-1 ) and phosphatidylinositol (112-284 g kg-1 ) were the main phospholipids. The content of saturated fatty acids in the phospholipids was significantly higher than that in triacylglycerols. CONCLUSION: Small variations in the chemical composition of eggs from seven different genotypes were observed. Significant differences in the fatty acid compositions of the main classes of phospholipids and the triacylglycerol fraction were established. © 2019 Society of Chemical Industry.


Assuntos
Galinhas/genética , Ovos/análise , Animais , Colesterol/análise , Gema de Ovo/química , Ácidos Graxos/análise , Genótipo , Nutrientes/análise , Fosfolipídeos/análise , Triglicerídeos/análise
7.
Meat Sci ; 156: 105-110, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31150937

RESUMO

Carcass and meat quality parameters of two different weights of pigs from the rustic breed Chato Murciano (Murcia Region, south-eastern Spain) were studied: light weight (LW, 147.98 ±â€¯3.78 kg live weight) and heavy weight (HW, 176.07 ±â€¯4.78 kg live weight). No weight-dependent effects were observed on carcass quality (carcass yield, length, back fat thickness) or on moisture, pH, and the L* and a* coordinates. The HW group had a higher fat content and cholesterol values than the LW group. None of the fatty acids analysed in the respective meats showed differences between groups (P > .05), except C16:0, C18:2 and C18:3. The HW group had higher values for unsaturated and polyunsaturated fatty acids and the n6/n3 index than LW, and lower values for saturated fat and the Saturated/Polyunsaturated fat ratio and a lower Atherogenesis Index. In conclusion, meat and fat quality are influenced by the final live weight of Chato Murciano pigs.


Assuntos
Peso Corporal , Ácidos Graxos/análise , Carne Vermelha/análise , Tecido Adiposo , Animais , Composição Corporal , Colesterol/análise , Cor , Masculino , Músculo Esquelético/química , Espanha , Suínos
8.
Chemistry ; 25(41): 9629-9633, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31090967

RESUMO

We here report a simple and efficient strategy of fabricating artificial multi-enzyme complex (MEC) based on the integration of natural enzyme with catalytic compartment. As a proof of concept, this strategy was demonstrated by selecting cholesterol oxidase (ChOx) and CeIII -based nanoscale coordination polymer (Ce-NCP) with peroxidase-like activity as the models, which forms ChOx@Ce-NCP. Benefitting from the confinement and sheltering effects of Ce-NCP, superior cascade activity and stability in harsh environments were achieved in ChOx@Ce-NCP. Meanwhile, the distinct advantage of ChOx@Ce-NCP has also been highlighted by its negligible substrate inhibition effect and adjustable mass ratio of building blocks. Upon the doping of TbIII in ChOx@Ce-NCP, a luminescent artificial MEC (ChOx@Ce-NCP:Tb) was further fabricated to drive a cascade amplified time-resolved fluorescent assay within a confined space, showing high sensitivity and specificity toward cholesterol.


Assuntos
Técnicas Biossensoriais/métodos , Cério/química , Colesterol Oxidase/química , Colesterol/sangue , Substâncias Luminescentes/química , Polímeros/química , Catálise , Colesterol/análise , Colorimetria/métodos , Enzimas Imobilizadas/química , Humanos , Complexos Multienzimáticos/química , Peroxidase/química , Espectrometria de Fluorescência/métodos
9.
Med Sci Monit ; 25: 3417-3424, 2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31068569

RESUMO

BACKGROUND Nowadays, more than 170 million patients suffer from diabetes mellitus worldwide. This study aimed to investigate the effects of sleeve gastrectomy (SG) and ileal transposition (IT) surgery on the control of diabetes. MATERIAL AND METHODS Goto-Kakizaki rats were used to establish type 2 diabetes models and undergo SG or IT surgery. At 2 months post-surgery, insulin, glucose, triglycerides (TG), total cholesterol (TC), glucose tolerance, glucagon-like peptide-1 (GLP-1) levels, and insulin sensitivity were evaluated. RESULTS SG significantly shortened operative time and post-operative recovery time compared to IT surgery (P<0.05). SG and IT surgery resulted in significantly induced weight loss, significantly decreased levels of glucose, and significantly enhanced levels of Ghrelin compared the Sham surgery group (P<0.001). SG and IT surgery resulted in significantly increased GLP-1 levels compared to Sham surgery (P<0.001). SG resulted in better reduction of oral glucose tolerance test (OGTT) glucose compared to IT surgery (P<0.05). SG and IT surgery significantly upregulated insulin tolerance test (ITT) levels compared to Sham surgery (P<0.001). SG induced better reductions in TC and TG compared to IT surgery (P<0.05). CONCLUSIONS In non-obese rats with spontaneous diabetes, both SG and IT surgery were found to control diabetes by regulating body weight and levels of glucose, Ghrelin, GLP-1, OGTT glucose, insulin, TC, and TG. Moreover, SG demonstrated advantages of shorter operative time, shorter post-operative recovery time, and better control of diabetes compared to IT surgery.


Assuntos
Diabetes Mellitus Experimental/terapia , Gastrectomia/métodos , Íleo/cirurgia , Anastomose Cirúrgica/métodos , Animais , Glicemia/análise , Peso Corporal/fisiologia , Colesterol/análise , Colesterol/sangue , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/terapia , Modelos Animais de Doenças , Grelina/análise , Grelina/sangue , Peptídeo 1 Semelhante ao Glucagon/análise , Peptídeo 1 Semelhante ao Glucagon/sangue , Glucose/metabolismo , Teste de Tolerância a Glucose , Insulina/sangue , Resistência à Insulina , Masculino , Ratos , Ratos Endogâmicos , Perda de Peso
10.
Se Pu ; 37(5): 539-546, 2019 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-31070338

RESUMO

An analytical method was developed for the simultaneous determination of five cholesterol oxidation products (COPs) in marinated pig feet and hocks by gas chromatography-mass spectrometry (GC-MS). The five COPs were 7 ß -hydroxycholesterol, cholesterol-5 α,6 α -epoxide, 3 ß,5 α,6 ß -trihydroxycholestane, 25-hydroxycholesterol and 7-ketocholesterol. The sample was extracted with methanol-chloroform (1:2, v/v) and purified by solid phase extraction. Subsequently, the sample was collected for derivatization by N,O-bis(trimethylsilyl)acetamide-trimethyl chlorosilane-1-trimethylsilyllimidazole (3:2:3, v/v/v) (Sylon BTZ). The column temperature was properly programmed, and the selected ion monitoring (SIM) mode was used for the determination of COPs. Under optimum conditions, the five COPs were well separated within 22 min with good separation. The linear range of the five COPs met the requirement of determination, and the average recoveries of the five COPs spiked in the pork samples at three levels were 61.16%-96.96% with relative standard deviations (RSDs) no more than 7.80% (n=3). The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 0.02-47.07 ng/g and 0.06-156.90 ng/g, respectively. This method has a wide line arrange and high sensitivity, and has been successfully applied to the analysis of COPs in actual samples.


Assuntos
Colesterol/análise , Carne Vermelha/análise , Tarso Animal , Animais , Cromatografia Gasosa-Espectrometria de Massas , Oxirredução , Extração em Fase Sólida , Suínos
11.
Anal Chim Acta ; 1070: 80-87, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31103170

RESUMO

The ultrasensitive bioassays are increasingly demanded for disease diagnosis and environmental monitoring. The combined unique natures of the components in nanocomposites have led to their wide applications in bioanalysis. In the current study, a simple strategy for preparing polyethyleneimine-functionalized reduced graphene oxide-hemin-bovine serum albumin (PEI-rGO-Hemin-BSA) nanocomposites as peroxidase mimetics was demonstrated. The developed nanocomposites of PEI-rGO-Hemin-BSA showed an excellent peroxidase-like activity. Importantly, through the glutaradelhyde crosslinking, PEI-rGO-Hemin-BSA could be further simply combined with various oxidases such as glucose oxidase, cholesterol oxidase, lactate oxidase and choline oxidase for the detection and quantitative measurement of multiple metabolites including glucose, cholesterol, l-lactate, and choline. The developed detection strategy, which is sensitive, convenient, low-costed, and in tiny sample consumption, could be expected wide applications in the disease diagnosis and management of metabolite disorders.


Assuntos
Grafite/química , Hemina/química , Doenças Metabólicas/diagnóstico , Nanocompostos/química , Peroxidase/metabolismo , Polietilenoimina/química , Soroalbumina Bovina/química , Animais , Bovinos , Colesterol/análise , Colesterol/metabolismo , Colina/análise , Colina/metabolismo , Glucose/análise , Glucose/metabolismo , Humanos , Ácido Láctico/análise , Ácido Láctico/metabolismo , Oxirredução
12.
Talanta ; 200: 293-299, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31036187

RESUMO

The co-immobilization of two enzymes onto single support commonly exhibits low efficiency due to the competition against limited sites. Water-stable metal-organic frameworks (MOFs) [i.e., PCN-333(Al)] with a high surface area and ultra-large cavities were employed to efficiently adsorb cholesterol oxidase (ChOx) and encapsulate horseradish peroxidase (HRP), respectively. The prepared PCN-333/ChOx&HRP was characterized through SEM, XRD, confocal microscopy, N2 adsorption isotherms, and thermal gravity analysis (TGA). The high surface area and high concentration of mesoporous cages resulted in the high loadings of both ChOx and HRP. The absorbed ChOx and the encapsulated HRP presented excellent activities without additional chemical modification. The immobilized enzymes were stable against protease digestion, organic solvents, temperature changes, and pH variation. Thus, a colorimetric biosensor for cholesterol detection was fabricated depending on cascade catalytic reactions of the immobilized bi-enzymes. An extended linear range from 0.0 to 40.0 µM with a low detection limit of 0.6 µM was obtained using the biosensor. The co-immobilization of the enzymes onto the surface and into the mesopores of MOFs provided a new and excellent platform for the development of highly stable and sensitive colorimetric biosensors.


Assuntos
Técnicas Biossensoriais , Colesterol Oxidase/química , Colesterol/análise , Colorimetria , Peroxidase do Rábano Silvestre/química , Estruturas Metalorgânicas/química , Adsorção , Colesterol Oxidase/metabolismo , Peroxidase do Rábano Silvestre/metabolismo , Tamanho da Partícula , Propriedades de Superfície
13.
Maturitas ; 125: 41-44, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31133215

RESUMO

Measurement of serum testosterone (T) level is of utmost importance for the evaluation of hypogonadism in men and androgen excess in women. Despite the advances in steroid hormone assessment, substantial variability exists regarding measurement of T concentrations. Several factors affect T measurement in men, including circadian rhythms, intra-individual daily variability and transient stressors, while T concentrations in women vary mainly according to the phase of the menstrual cycle. Most of the available immunoassays lack the required accuracy when dealing with T concentrations at the lower end of the normal range for men and across the entire range for females. Consequently, there is no universally accepted lower T threshold for healthy adult men and most immunoassays fail to detect states of mild androgen excess in women. Mass spectrometry is considered the gold-standard method for T measurement; however, due to its complexity and cost, it has not been widely adopted. To increase accuracy, T in men should be measured with a fasting morning sample and repeated if the level is found to be low; in women, measurement must be performed at the follicular phase of the cycle. In both cases, borderline results may be clarified by the assessment of free testosterone (fT). Since most fT assays are unreliable, calculated surrogates should be used instead. Collaborative efforts have been undertaken, with rigorous internal and external quality controls and the establishment of reference methods, to harmonise the commercial assays.


Assuntos
Hipogonadismo/sangue , Testosterona/sangue , Adulto , Bioensaio , Colesterol/análise , Feminino , Fase Folicular/sangue , Humanos , Imunoensaio , Masculino , Espectrometria de Massas , Ciclo Menstrual , Valores de Referência , Reprodutibilidade dos Testes , Fatores Sexuais , Adulto Jovem
14.
J Dairy Sci ; 102(7): 5933-5944, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31030910

RESUMO

The fortification of milk with phytosterols is an increasingly common practice to enhance the sterol profile and offer consumers potential health benefits. This study investigated whether cattle feed can influence the profile of phytosterols and cholesterol in the milk produced as an alternative to direct fortification of milk. Five experiments were performed using feeds commonly used by Australian dairy farmers and selected formulated rumen-protected feeds. Statistical significances were observed for some individual plant sterols and cholesterol in milk under these differing feeding regimens compared with the respective controls. In the case of the phytosterols, where the daily recommended consumption is typically 2 g per day, the total phytosterols were <0.12 mg/100 mL of milk. An experiment using a rumen-protected feed with high phytosterol levels suggested a decreased transfer of cholesterol to the milk by as much as 20%, although further work is required to confirm these preliminary results. Overall, the study suggests that different feeding practices have minimal effect on the resulting sterol profile of the milk.


Assuntos
Ração Animal/análise , Indústria de Laticínios/métodos , Dieta/veterinária , Leite/química , Animais , Biofortificação , Bovinos , Colesterol/análise , Feminino , Fitosteróis/análise
15.
Poult Sci ; 98(7): 3067-3080, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30938805

RESUMO

This study was performed to determine the slaughter and carcass traits, meat color, pH, nutrient composition, fatty acids, amino acids, and cholesterol content of local Turkish goose varieties. In the present study, a total of 80 geese from white, black, gray, and multicolored varieties were reared in an extensive production system at 28 wk of age. Quality analysis was performed on breast and thigh meat, as well as on the skin and abdominal fat of studied geese. Slaughter and carcass weights were significantly higher in the white variety (P < 0.01). Measured abdominal fat was lower in black and gray geese. Furthermore, the color and pH values were significantly different on breast, thigh, and skin among varieties. Additionally, measured breast meat b*, thigh meat L*, and b* values were higher in male geese. Breast meat protein levels were lower in black and gray geese, and also lower in the thigh meat of gray geese. Total breast meat amino acids were lower in white geese, and the amino acid content of thigh meat was lower in multicolored varieties. Moreover, differences in fatty acid composition were found among the studied varieties. However, cholesterol levels did not differ for either sex or variety.


Assuntos
Gansos , Carne/análise , Tecido Adiposo , Aminoácidos/análise , Criação de Animais Domésticos/métodos , Animais , Composição Corporal , Colesterol/análise , Cor , Ácidos Graxos/análise , Feminino , Masculino , Proteínas Musculares/análise , Valor Nutritivo , Especificidade da Espécie , Turquia
16.
Chem Commun (Camb) ; 55(34): 5021-5024, 2019 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-30968895

RESUMO

To address the issues allied with cardiovascular diseases and the increased prevalence of hypertension, a novel electrochemical sensor was developed based on poly(ionic liquid) [PVIM]-cobalt polyoxometalate (Co5POM) supported on carbonaceous materials (MNCs) for the highly selective and ultrasensitive non-enzymatic detection of cholesterol. The novel composite exhibits excellent selectivity and sensitivity towards the non-enzymatic electrochemical detection of cholesterol with a low detection limit of 1 fM (1 × 10-15 M), a response time of ∼5 s, and a wide detection range of 1 fM-5 mM with two linear ranges of 1 fM-200 nM and 0.5 µM-5 mM with sensitivities of 210 and 64 µA µM-1 cm-2, respectively, even in the presence of ascorbic acid, glucose and uric acid. The proposed sensor was tested with a human blood serum sample at physiological pH in a physiologically relevant concentration range. A novel flexible sensor was developed with PVIM-Co5POM/MNC coated over Whatman filter paper and this shows a high sensitivity with a wide detection range of 1 fM-5 mM.


Assuntos
Carbono/química , Colesterol/análise , Técnicas Eletroquímicas , Líquidos Iônicos/química , Nitrogênio/química , Compostos de Tungstênio/química , Eletrodos , Tamanho da Partícula , Porosidade , Propriedades de Superfície
17.
Trop Anim Health Prod ; 51(7): 2025-2035, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31011922

RESUMO

The present study is aimed at comparing the fatty acid profiles, cholesterol, and atherogenicity index of Bactrian and dromedary camel meat by using discriminant analysis to identify the more discriminating fatty acids. Six muscles were sampled from nine Bactrian and nine dromedary camels and analyzed for fatty acid parameters and cholesterol content. The mean fatty acid profiles differed in higher proportion between species than between muscles. The main discriminating fatty acids between species (100% well-classed samples) were C15:0, C17:1, C14:1, C20:0, and C18:0. A significant difference was also observed in cholesterol content, with more cholesterol in Bactrian meat (53.6 ± 12.5 mg/100 g) compared to dromedary meat (49.4 ± 11.2 mg/100 g). However, the atherogenicity index was lower in Bactrian meat (1.196 ± 0.148) than in dromedary meat (1.379 ± 0.109). Despite the dietetic interest in camel meat due to its low cholesterol and low-fat content, the high atherogenicity index compared to other red meat appeared as an unfavorable argument.


Assuntos
Camelus , Colesterol/análise , Ácidos Graxos/análise , Carne/análise , Animais , Dieta Aterogênica , Análise Discriminante , Especificidade da Espécie
18.
J Clin Pathol ; 72(8): 529-535, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31028099

RESUMO

AIMS: In order to improve diagnostics in pleural effusions, additional value of effusion cholesterol, carcinoembryonic antigen (CEA) and syndecan-2 assays to cytology was studied. METHODS: Biomarkers were measured in effusion supernatants from 247 patients, of whom 126 had malignant pleural involvement, and their additional diagnostic efficacy to cytology was assessed. RESULTS: Syndecan-2 measurement, although gave detectable concentrations in all effusions with highest median value in mesotheliomas, was non-discriminative between different pathological conditions. CEA concentrations exceeding 5 ng/mL cut-off point indicated carcinomas, regardless of pleural involvement, which gave a sensitivity of 62% and specificity of 100% for carcinoma. Cholesterol concentration over 1.21 mmol/L cut-off value indicated neoplastic pleural involvement with 99% sensitivity and 'merely' 69% specificity, the latter mainly due to raised levels being associated also with benign inflammatory effusions. Combined CEA and cholesterol determinations increased the sensitivity for diagnosing carcinomatosis from 70% with cytology alone to 84% and established the correct diagnosis in 16 of 31 carcinomatosis cases with inconclusive cytology. Cholesterol measurement alone, with elevated level, in combination with absence of substantial number of inflammatory cells in effusion sediment proved to be a magnificent marker for neoplastic pleural involvement with 99% efficacy, and recognised all 36 such cases with inconclusive cytology. CONCLUSIONS: Simultaneous measurement of CEA and cholesterol concentrations in effusion, or at least cholesterol alone, in combination with non-inflammatory fluid cytology, provides additional specific information about neoplastic pleural involvement, and can therefore be used as an adjunct to cytology, above all, in inconclusive cases.


Assuntos
Biomarcadores Tumorais/análise , Antígeno Carcinoembrionário/análise , Colesterol/análise , Derrame Pleural Maligno/química , Sindecana-2/análise , Adulto , Idoso , Idoso de 80 Anos ou mais , Citodiagnóstico , Diagnóstico Diferencial , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Derrame Pleural Maligno/patologia , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Adulto Jovem
19.
Med Sci Monit ; 25: 2976-2983, 2019 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-31011149

RESUMO

BACKGROUND The aim of this study was to assess the hypoglycemic effect of Cyclocarya paliurus extract (CPE) on diabetes mellitus (DM) mice. MATERIAL AND METHODS A DM mouse model was established to test FBG, TC, and TG. The DM mice were divided into 3 groups: a DM group, a DM+CPE (0.5 g/Kg) group, and a DM+CPE (1.0 g/Kg) group. The FBG and body weight were measured. The glucose tolerance ability was determined by OGTT test. FINS was measured to calculate ISI and IRI. Serum MDA, SOD, and GSH-Px levels were detected. NIT-1 cells were cultured in vitro and divided into 4 groups: a control group, a STZ group, a STZ+CPE (80 µg/mL) group, and a STZ+CPE (160 µg/mL) group. Cell apoptosis and ROS content were assessed by flow cytometry. Cell proliferation was detected by EdU staining. RESULTS Compared with the control group, FBG, TC, and TG were significantly increased in the DM group. CPE gavage obviously reduced FBG level, increased body weight, enhanced glucose tolerance, elevated FINS level and ISI, and reduced IRI, all in a dose-dependent manner. CPE gavage reduced serum MDA content and increased SOD and GSH-Px enzyme activities in DM mice. STZ markedly enhanced ROS production, induced apoptosis, and inhibited proliferation in NIT-1 cells. CPE treatment clearly reduced ROS production and apoptosis, enhanced cell proliferation, and alleviated STZ damage to NIT-1 cells. CONCLUSIONS CPE has the effects of decreasing blood glucose and insulin resistance, and enhancing glucose tolerance in DM mice, which may be related to its effects of reducing oxidation and reduced apoptosis, and relieving STZ in pancreatic beta cell injury.


Assuntos
Diabetes Mellitus Experimental/tratamento farmacológico , Juglandaceae/química , Extratos Vegetais/farmacologia , Animais , Antioxidantes/farmacologia , Glicemia/análise , Glicemia/efeitos dos fármacos , Colesterol/análise , Colesterol/sangue , Diabetes Mellitus Tipo 2/tratamento farmacológico , Modelos Animais de Doenças , Feminino , Hipoglicemiantes/farmacologia , Insulina/metabolismo , Resistência à Insulina , Células Secretoras de Insulina/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Fitoterapia/métodos , Folhas de Planta/química , Triglicerídeos/análise , Triglicerídeos/sangue
20.
Meat Sci ; 154: 61-68, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31004941

RESUMO

This study investigated the carcass characteristics, physico-chemical properties, storage stability and cholesterol content of meat from goats fed with different levels of naturally-produced lovastatin used to mitigate enteric methane production. Twenty intact Saanen male goats of 5-6 months old with initial live weight of 25.8 ±â€¯4.0 kg were randomly allotted into four dietary treatments containing 0 (Control), 2 (Low), 4 (Medium) and 6 mg (High) per kg live weight (LW) of naturally-produced lovastatin for 12 consecutive weeks. No differences were found in all the parameters measured except for full LW, hot and cold carcass weight, shear force, color and cholesterol content among the treatment groups. Aging had significant effects on all the parameters measured in this study except a* (redness) of meat. Meat samples in the Medium and High treatments were of higher lightness and yellowness, more tender and lower cholesterol levels. We conclude that, in addition to mitigate enteric methane emissions, dietary supplementation of naturally-produced lovastatin at 4 mg/kg LW could be a feasible feeding strategy to produce tender meat containing lower cholesterol.


Assuntos
Composição Corporal/efeitos dos fármacos , Dieta/veterinária , Lovastatina/farmacologia , Carne/análise , Ração Animal/análise , Animais , Produtos Biológicos/administração & dosagem , Produtos Biológicos/farmacologia , Peso Corporal/efeitos dos fármacos , Colesterol/análise , Cor , Cabras , Lovastatina/administração & dosagem , Masculino , Metano/metabolismo , Músculo Esquelético/química , Resistência ao Cisalhamento/efeitos dos fármacos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA