Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.486
Filtrar
1.
Biochem Med (Zagreb) ; 30(1): 010703, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31839723

RESUMO

Introduction: Circulating microRNAs (miRNAs) are emerging as potential biomarkers. However, the lack of preanalytical and analytical standardization limits their use. The aim of this study was to determine the expression of different miRNAs in plasma according to different collection and storage conditions. Materials and methods: Venous blood from 10 volunteers was collected in tubes spray-coated with dipotassium salt of ethylendiaminetetraacetic acid, either with (plasma-preparation tube, PPT) or without (K2EDTA) gel separator. Platelet-poor plasma (PPP) was also obtained from K2EDTA plasma. After storage under different conditions, miRNA-enriched total RNA was isolated from plasma and reverse transcribed. A panel of 179 miRNAs was assayed by quantitative polymerase chain reaction and the results were analysed by GenEx software. Detectability and stability of miRNAs were determined. Results: The number of undetected miRNAs was: 18, 24, and 22 in PPT; 83, 43, and 20 in K2EDTA; and 76, 106, and 104 in PPP samples, for plasma immediately frozen at - 80°C and plasma stored for 24h at room temperature or 4°C, respectively. Circulating miRNA expression in PPT samples was not affected by storage delay or temperature, while the percentage of up- and down-regulated miRNA in K2EDTA and PPP samples ranged from 2%, and 1% to 7%, and 5%, respectively. Conclusions: Sample matrix, temperature and delay in storage strongly influence the expression level of plasma miRNAs. Our results indicate PPT tubes as the most suitable matrix to improve total miRNA detectability and stability, independently of temperature.


Assuntos
Coleta de Amostras Sanguíneas/métodos , MicroRNA Circulante/sangue , Adulto , Biomarcadores/sangue , Plaquetas/citologia , Coleta de Amostras Sanguíneas/instrumentação , MicroRNA Circulante/isolamento & purificação , MicroRNA Circulante/metabolismo , Humanos , Masculino , Fase Pré-Analítica , Temperatura Ambiente
2.
Biochem Med (Zagreb) ; 30(1): 010704, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31839724

RESUMO

Introduction: Diabetes mellitus (DM) is one of the most prevalent diseases worldwide. The objective of this study was to find out under what preanalytical conditions routine and diagnostic glucose tests are performed across Spanish laboratories; and also what criteria are used for DM diagnosis. Materials and methods: An online survey was performed by the Commission on Quality Assurance in the Extra-Analytical Phase of the Spanish Society of Laboratory Medicine (SEQC-ML). Access to the questionnaire was available on the home page of the SEQC-ML website during the period April-July 2018. Data analysis was conducted with the IBM SPSS© Statistics (version 20.0) program. Results: A total of 96 valid surveys were obtained. Most laboratories were in public ownership, serving hospital and primary care patients, with high and medium workloads, and a predominance of mixed routine-urgent glucose testing. Serum tubes were the most used for routine glucose analysis (92%) and DM diagnosis (54%); followed by lithium-heparin plasma tubes (62%), intended primarily for urgent glucose testing; point-of-care testing devices were used by 37%; and plasma tubes with a glycolysis inhibitor, mainly sodium fluoride, by 19%. Laboratories used the cut-off values and criteria recognized worldwide for DM diagnosis in adults and glucose-impaired tolerance, but diverged in terms of fasting plasma glucose and gestational DM criteria. Conclusion: Preanalytical processing of routine and DM diagnostic glucose testing in Spain does not allow a significant, non-quantified influence of glycolysis on the results to be ruled out. Possible adverse consequences include a delay in diagnosis and possible under-treatment.


Assuntos
Glicemia/análise , Coleta de Amostras Sanguíneas/métodos , Coleta de Amostras Sanguíneas/instrumentação , Diabetes Mellitus/diagnóstico , Humanos , Laboratórios Hospitalares/normas , Fase Pré-Analítica , Espanha , Inquéritos e Questionários
3.
BMC Res Notes ; 12(1): 511, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31416482

RESUMO

OBJECTIVE: Type I interferons (IFN) have important roles in many immune-mediated inflammatory diseases (IMIDs) and are a relatively new therapeutic target. Direct detection of type I IFNs has proved challenging, thus their presence is often inferred from the expression of interferon-stimulated genes (ISGs) and calculation of an interferon score (IS). The objective of this research was to determine if the expression of six common ISGs and subsequent IS were comparable when RNA was derived from the Tempus and PAXgene whole blood RNA collection systems. RESULTS: Whole blood was obtained from ten healthy adults, incubated ex vivo in the absence and presence of recombinant human IFNα then divided into PAXgene and Tempus tubes. Despite reports of tube-specific patterns of gene expression, quantitative PCR (qPCR) analysis revealed no significant differences between PAXgene and Tempus tubes in either the homeostatic or IFNα-induced expression of six ISGs (IFI27, IFI44L, IFIT1, ISG15, RSAD2, SIGLEC1). Overall there was a strong correlation in the IS between unstimulated (r = 0.92, p = 0.0005) and IFNα-stimulated (r = 0.71, p = 0.0268) samples derived from the PAXgene and Tempus tubes.


Assuntos
Coleta de Amostras Sanguíneas/métodos , Perfilação da Expressão Gênica/métodos , Interferon Tipo I/genética , RNA/genética , Adulto , Coleta de Amostras Sanguíneas/instrumentação , Feminino , Voluntários Saudáveis , Humanos , Interferon Tipo I/sangue , Masculino , RNA/sangue , RNA/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Adulto Jovem
4.
Clin Chim Acta ; 496: 18-24, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31201816

RESUMO

INTRODUCTION: The BD Barricor tube uses a novel mechanical separator designed to eliminate gel artifacts, decrease cellular contamination, and improve stability. Here, we evaluated the Barricor tube as a possible replacement for PST using Beckman Coulter analyzers under both optimal, alternative, and suboptimal centrifugation conditions based on BD recommendations. METHODS: Paired PST and Barricor samples were collected from 4 local hospitals and processed based on site-specific preanalytical systems involving automated or manual centrifugation. Centrifugation conditions ranged from 1912 ×g for 10 min (suboptimal), 2060 g for 10 min (alternative), and 4000 ×g for 3 or 10 min (optimal). Tube volume (4.5 vs. 5.5 ml) was also assessed. Forty-three chemistry and immunochemistry analytes were measured on Beckman Coulter DxC and DxI analyzers. RESULTS: Using an automated preanlaytical system with suboptimal spin conditions, no bias between PST and Barricor was observed for all analytes tested except lactate dehydrogenase (LD). Further investigation revealed significant increase in LD when Barricor was spun for 10 min at 1912, 2060 and 4000 ×g, ranging from +7.4-19.4% vs. PST across the entire measurement interval (87-493 U/l). Smaller tube volume was also associated with higher LD. Differences in LD occurred despite no change in other hemolysis markers such as potassium, phosphate, and AST. CONCLUSIONS: LD is most sensitive to varying centrifugation conditions (time and speed) in Barricor tubes. We recommend that BD centrifugation protocols should be closely evaluated to determine if Barricor is equivalent to PST under local preanalytical configurations.


Assuntos
Análise Química do Sangue , Coleta de Amostras Sanguíneas/instrumentação , Imunoensaio , L-Lactato Desidrogenase/sangue , Plasma/química , Humanos
5.
Eur Radiol ; 29(11): 6330-6335, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31025064

RESUMO

Adrenal vein sampling (AVS) is the key test for subtyping patients with primary aldosteronism (PA) before referring those with unilateral disease for laparoscopic unilateral adrenalectomy. However, it is still not systematically used, despite guidelines recommendations, because it is still considered as an invasive, risky, and challenging procedure. Simultaneous bilateral catheterization is believed to add technical difficulties inherent with attempting to catheterize both adrenal veins at the same time, but can be useful to minimize differences between the sides due to timing. We herein report on the protocol for routine clinical use. Tips for preparation of the patient as well as optimal catheterization of adrenal veins and sampling are provided to propose a protocol that is easy, safe, and reliable. Key Points • Adrenal vein sampling is the reference standard in the case of primary aldosteronism to detect the hyper-functioning side and allow subsequent treatment. • Simultaneous bilateral adrenal vein sampling avoids bias related to sampling timing. • Some technical suggestions concerning patient preparation and catheterization are proposed to make simultaneous adrenal vein sampling easier and safer.


Assuntos
Glândulas Suprarrenais/irrigação sanguínea , Coleta de Amostras Sanguíneas/métodos , Cateterismo , Hiperaldosteronismo/diagnóstico , Adulto , Coleta de Amostras Sanguíneas/instrumentação , Cateterismo/efeitos adversos , Cateterismo/instrumentação , Cateterismo/métodos , Protocolos Clínicos/normas , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
6.
Bioanalysis ; 11(6): 525-532, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30973017

RESUMO

Following the completion of a detailed experimental protocol into the potential inhomogeneity of capillary liquid microsamples, which was performed at seven European Bioanalysis Forum member companies, the summary and conclusion on the data are reported here. It has been demonstrated that it is possible to generate homogeneous samples using these microsampling techniques; that the resultant microsamples can be accurate and precise and that capillary liquid microsampling data can be consistent with conventional larger volume plasma samples. However, the data contain some variability which is contributed to by the different range of experiences that each investigating site had with these techniques. Therefore, knowledge of the compounds, well-designed experiments and experience with these techniques are essential for the delivery of high quality data.


Assuntos
Análise Química do Sangue/métodos , Coleta de Amostras Sanguíneas/métodos , Análise Química do Sangue/normas , Coleta de Amostras Sanguíneas/instrumentação , Coleta de Amostras Sanguíneas/normas , Europa (Continente) , Humanos , Preparações Farmacêuticas/sangue , Reprodutibilidade dos Testes
7.
Bioanalysis ; 11(6): 533-542, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30973018

RESUMO

Aim: Microsampling in preclinical pharmacokinetics (PK) studies is currently widely adopted across the pharmaceutical industry. Materials & methods: The European Bioanalysis Forum liquid microsampling consortium member companies assessed the accuracy and precision of handheld pipettes and microcapillaries at volumes of less than 10 µl. The following key factors on pipetting performance were also evaluated: Pipette type (positive displacement, air displacement and microcapillary), experience of user and the liquid type. Water was selected as a best-case scenario for accuracy and precision determination and blood plasma as a 'real world' bioanalysis sample type. Conclusion: Accuracy and precision on the pipetted volume decreased at lower volumes and experienced laboratory technicians performed better compared with the infrequent users. With respect to the pipetting devices used, microcapillaries showed better or equivalent accuracy and precision compared with handheld pipettes across the volume range 1-8 µl independent of the matrix used.


Assuntos
Análise Química do Sangue/métodos , Coleta de Amostras Sanguíneas/instrumentação , Análise Química do Sangue/normas , Coleta de Amostras Sanguíneas/normas , Europa (Continente) , Humanos , Preparações Farmacêuticas/sangue , Reprodutibilidade dos Testes
8.
Anal Bioanal Chem ; 411(13): 2767-2780, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30976894

RESUMO

Blood counting is one of the most commonly ordered clinical assays, and is often part of the basis for initial diagnosis and screening for disease. While substantial prior research has shown the ability of portable instruments to accurately produce blood counts through image- or flow-based cytometry, these methods require complex sample preparation using either costly commercial imaging chambers or complicated reagents. To address these issues, in this paper we developed a method to prepare trace volumes of whole blood aimed at portable blood counting. The strategy is based on pre-storing dry-form reagents and fabricating a specifically designed cell counter. In order to obtain total cell counts for red blood cells, platelets, and 3-part differentials of white blood cells, two parallel counting chambers with different depths are made from cost- and environmentally friendly materials using soft lithography. As little as 1 µl of whole blood is prepared with pre-stored reagents in centrifuge vials, whereas red blood cells are sphered and white blood cells are stained at the same time. Driven by the capillary force, prepared blood samples enter the hydrophilic chambers automatically. Monolayers of cells are formed when the blood dilution factors and the chamber depths are co-optimized. Combined with our previous custom-built instrument and automated analysis algorithm, the sample preparation strategy allows producing counting results with excellent agreement to a gold-standard clinical hematology instrument. The success of this preparation method may further advance applications of our technology for global use in low-resource settings where central hematology laboratories are not accessible. Graphical abstract Graphical Abstract.


Assuntos
Contagem de Células Sanguíneas/instrumentação , Imagem Óptica/instrumentação , Sistemas Automatizados de Assistência Junto ao Leito , Contagem de Células Sanguíneas/métodos , Coleta de Amostras Sanguíneas/instrumentação , Coleta de Amostras Sanguíneas/métodos , Desenho de Equipamento , Humanos , Indicadores e Reagentes , Microscopia/instrumentação , Microscopia/métodos , Imagem Óptica/métodos , Tamanho da Amostra
10.
Biosens Bioelectron ; 132: 38-46, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30851494

RESUMO

Early diagnosis of dengue biomarkers by employing a technology that is less labor- and time-intensive and offers higher sensitivity and lower limits of detection would find great significance in the developing world. Here, we report the development of a biosensor that exploits the localized surface plasmon resonance (LSPR) effect of silver nanostructures, created via thermal annealing of thin metal film, to detect dengue NS1 antigen, which appears as early as the onset of infection. The biosensor integrates membrane-based blood-plasma separation to develop lab-on-chip device that facilitates rapid diagnosis (within 30 min) of dengue NS1 antigen from a small volume (10 µL) of whole blood. The refractive index (RI) sensitivity of the LSPR biosensor was verified by using aqueous glycerol (0-100 wt%) which showed that it is sufficiently sensitive to detect 10-3 change in RI, which is comparable to that observed with protein-protein interaction. The RI sensitivity was utilized to demonstrate protein binding by using bovine serum albumin and detection of antibody-antigen immune reaction by binding human chorionic gonadotropin antigen to immunoglobulin antibody immobilized in our LSPR biosensor. Next, we demonstrated the detection of NS1 in plasma obtained via centrifugation and in plasma separated on-chip. From 10 µL of whole blood spiked with NS1 antigen, our biosensor reliably detects 0.06 µg/mL of NS1, which lies within the clinical limit observed during the first seven days of infection, with a sensitivity of 9 nm/(µg/mL). These results confirm that the proposed LSPR biosensor can potentially be used in point-of-care dengue diagnostics.


Assuntos
Vírus da Dengue/isolamento & purificação , Dengue/sangue , Nanoestruturas/química , Prata/química , Ressonância de Plasmônio de Superfície/instrumentação , Proteínas não Estruturais Virais/sangue , Adulto , Anticorpos Imobilizados/química , Coleta de Amostras Sanguíneas/instrumentação , Dengue/diagnóstico , Humanos , Dispositivos Lab-On-A-Chip , Limite de Detecção
11.
Drug Test Anal ; 11(7): 931-936, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30901507

RESUMO

Serum insulin-like growth factor-1 (IGF-1), procollagen type III N-terminal peptide (PIIINP), and human growth hormone (hGH) isoforms were analyzed in identical serum samples collected into BD Vacutainer® SST and BD Vacutainer® SST-II Advance serum separator tubes. Comparing the serum collected into each tube, measurement correlation was high (R2  > 0.83) and percent bias was minimal (<|3.2%|) for all analytes measured using World Anti-Doping Agency (WADA)-approved tests. As such, it is recommended that both SST and SST-II Advance tubes can be used interchangeably for anti-doping purposes.


Assuntos
Coleta de Amostras Sanguíneas/métodos , Hormônio do Crescimento Humano/sangue , Fator de Crescimento Insulin-Like I/análise , Fragmentos de Peptídeos/sangue , Pró-Colágeno/sangue , Coleta de Amostras Sanguíneas/instrumentação , Doping nos Esportes , Humanos , Proteínas Recombinantes/sangue , Espectrometria de Massas em Tandem/métodos
12.
Int J Mol Sci ; 20(3)2019 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-30736351

RESUMO

In the last decade, circulating nucleic acids such as microRNAs (miRNAs) and cell-free DNA (cfDNA) have become increasingly important in serving as potential novel biomarkers for a variety of human diseases. If cell-free nucleic acids are to become routinely used in diagnostics, the difference in plasma miRNA and cfDNA levels between healthy and diseased subjects must exceed pre-analytical and analytical variability. Until now, few studies have addressed the time limitations of pre-processing or explored the potential use of long-term blood storage tubes, which might need to be implemented in real-life diagnostics. In this study, we analyzed the stability of four breast cancer-associated miRNAs and two cancer-associated genes under various storage conditions, to test their limitations for potential application in clinical diagnostics. In two consecutive experiments, we tested the limits of conventional EDTA tubes, as well as long-term storage blood collection tubes (BCTs) from four different manufacturers. We found that circulating miRNAs are relatively stable when stored in EDTA monovettes for up to 12 h before processing. When stored in BCTs, circulating miRNAs and cfDNA are stable for up to 7 days, depending on the manufacturer. Norgen tubes were superior for cfDNA yield, while Streck tubes performed the worst in our study with hemolysis induction. In conclusion, plasma prepared from whole blood is suitable for the quantification of both cf-miRNAs and cfDNA simultaneously.


Assuntos
Coleta de Amostras Sanguíneas , Ácidos Nucleicos Livres , Biópsia Líquida , Biomarcadores , Preservação de Sangue , Coleta de Amostras Sanguíneas/instrumentação , Coleta de Amostras Sanguíneas/métodos , MicroRNA Circulante , Feminino , Humanos , Biópsia Líquida/métodos , Masculino , Reação em Cadeia da Polimerase , Estabilidade de RNA , Fatores de Tempo
13.
Clin Lab ; 65(1)2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30775901

RESUMO

BACKGROUND: Hemolysis is frequently reported in samples sent from emergency departments. In our study we aimed to compare the influence of invitro hemolysis on test results and hemolysis ratios of different blood drawing techniques (aspiration method and vacuum filling technique) used to draw blood from intravenous (IV) catheters in Emergency Department. Two techniques (aspiration vs. vacuum filling) used to draw blood into three different tubes (Sarstedt S-Monovette® 4.9 mL Serum Gel tube, BD 5 mL Vacutainer® Rapid Serum Tube (RST), and 5 mL Vacutainer® SST™II tube) and evaluated the effect of the hemolysis index of the sera on the tests analyzed. METHODS: In the emergency department blood was drawn from 128 consecutive patients into Sarstedt S-Monovette® 4.9 mL Serum Gel tubes using aspiration technique and also into BD 5 mL Vacutainer® Rapid Serum Tubes (RST) and 5 mL Vacutainer® SST™II tubes using vacuum filling technique. All the tests requested from the patients were analyzed on all tubes and the hemolysis index of all the tubes were also evaluated. RESULTS: As a result, the percentage of hemolysis encountered in S-Monovette® vs. SST and S-Monovette® vs. RST was 4.41% vs. 14.71% and 0% vs. 18.97%, respectively (p < 0.001, p < 0.001). In addition to this, the mean values of the test results for each assay in S-Monovette® tubes showed a significant difference when compared to RST and SST (p < 0.01). CKMB and LDH test results found in the tubes filled using the aspiration techniques (S-Monovette®) were statistically significantly lower than the results gathered from the tubes filled using vacuum filling technique (Vacutainer® RST and Vacutainer® SST) (p < 0.001). CONCLUSIONS: The test results and HI taken from the aspiration method seemed to be more reliable despite the presence of hemolysis.


Assuntos
Coleta de Amostras Sanguíneas/métodos , Cateterismo , Serviço Hospitalar de Emergência , Testes Hematológicos/métodos , Hemólise , Coleta de Amostras Sanguíneas/instrumentação , Coleta de Amostras Sanguíneas/estatística & dados numéricos , Testes Hematológicos/estatística & dados numéricos , Humanos , Reprodutibilidade dos Testes , Vácuo
14.
BMC Res Notes ; 12(1): 39, 2019 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-30658701

RESUMO

OBJECTIVE: Studies of mRNA and miRNA expression profiling increasingly use stabilized whole blood. Commercial RNA extraction kits do not provide information about the simultaneous recovery of both mRNA and miRNA. This study evaluated yield, quality, integrity and representation of mRNA and miRNA from whole blood stabilized in Tempus tubes using three RNA extraction kits; two filter-based (Tempus and Norgen) and one bead-based (MagMax; manual vs. semi-automated, and with and without DNase treatment). RESULTS: All RNA extraction kits and methods resulted in similar yields of mRNA (total RNA yield, quality, integrity and representation) whereas there were differences in yields of miRNA. MagMax, either manual or semi-automated, with or without DNase treatment, yielded 1.6-2.2-fold more miRNA than Tempus and Norgen kits. In addition, MagMax and Norgen methods gave greater than 12-fold more and 3.3-fold less enrichment of specific miRNA targets, respectively, in comparison to Tempus extraction reagents. This study identified MagMax kit for simultaneous recovery of both mRNA and miRNA from whole blood collected in Tempus tubes.


Assuntos
Coleta de Amostras Sanguíneas/instrumentação , Perfilação da Expressão Gênica , MicroRNAs/genética , RNA Mensageiro/genética , Coleta de Amostras Sanguíneas/métodos , Humanos , MicroRNAs/sangue , MicroRNAs/isolamento & purificação , RNA Mensageiro/sangue , RNA Mensageiro/isolamento & purificação , Reprodutibilidade dos Testes
15.
Br J Nurs ; 28(2): S24-S28, 2019 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-30673311

RESUMO

Safety lancets are used to collect capillary blood samples to test if neonates have rare but serious congenital conditions, such as sickle cell disease, cystic fibrosis, congenital hypothyroidism and inherited metabolic diseases. Blood samples are taken from the heel, but the procedure can cause the neonate pain or discomfort, as well as a risk of local trauma to the nerves and blood vessels, bleeding, infection and scarring. This article explores the need for blood sampling in neonates, discusses the procedure and outlines the types of lancets available. It describes the Neoheel Safety Lancet (Smiths Medical), whose features are designed to avoid pain and trauma during the procedure. Three case studies are included to describe its use in clinical practice.


Assuntos
Coleta de Amostras Sanguíneas/instrumentação , Dor/prevenção & controle , Coleta de Amostras Sanguíneas/efeitos adversos , Desenho de Equipamento , Calcanhar , Humanos , Recém-Nascido , Dor/etiologia , Segurança
16.
Bioanalysis ; 11(1): 21-32, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30525928

RESUMO

AIM: Trazodone (TZD) is used for the treatment of depression in adults and, off-label, as a sleep medication in adult and pediatric populations. The off-label use is well documented, however further clinical studies are needed to confirm its efficacy and safety for the treatment of sleep disorders. In this scenario, we developed a bioanalytical method to quantify low TZD concentrations in samples collected by capillary microsampling (CMS) to support dose finding, Good Laboratory Practice juvenile rat toxicokinetic and upcoming pediatric studies. METHODOLOGY: A method using only 8 µl of plasma was developed and successfully used for analyzing CMS samples from juvenile rats throughout toxicokinetic study. CONCLUSION: By harmoniously maximizing each analytical step, we achieved a sensitive method to quantify TZD in CMS samples.


Assuntos
Ansiolíticos/sangue , Coleta de Amostras Sanguíneas/métodos , Trazodona/sangue , Animais , Ansiolíticos/administração & dosagem , Coleta de Amostras Sanguíneas/instrumentação , Calibragem , Capilares , Cromatografia Líquida , Relação Dose-Resposta a Droga , Feminino , Masculino , Ratos , Padrões de Referência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectrometria de Massas em Tandem , Toxicocinética , Trazodona/administração & dosagem
17.
Am J Clin Pathol ; 151(2): 164-170, 2019 01 07.
Artigo em Inglês | MEDLINE | ID: mdl-30260386

RESUMO

Objectives: Underfilling of blood culture bottles decreases the sensitivity of the culture. We attempt to increase average blood culture fill volumes (ABCFVs) through an educational program. Methods: Partnerships were established with four hospital units (surgical intensive care unit [SICU], medical intensive care unit [MICU], medical intermediate care unit [MIMCU], and hematology and oncology unit [HEME/ONC]). ABCFVs were continuously tracked and communicated to each unit monthly. Educational sessions were provided to each unit. Results: ABCFVs for the SICU, MICU, MIMCU, and HEME/ONC were 4.8, 5.0, 5.0, and 6.3 mL/bottle, respectively. After the final education session, the SICU, MICU, MIMCU, and HEME/ONC were able to maintain an ABCFV of 6.8, 8.1, 7.9, and 8.2 mL/bottle, respectively. Conclusions: Partnering with a specific unit and providing monthly volume reports with educational sessions has a direct positive correlation on increasing ABCFVs. Increasing ABCFVs has the potential to decrease false-negative blood cultures, time to detection of positive blood cultures, and time to appropriate and specific antimicrobial therapy, as well as improve patient outcomes in high-acuity patient care units.


Assuntos
Hemocultura/tendências , Coleta de Amostras Sanguíneas/tendências , Modelos Estatísticos , Software , Hemocultura/instrumentação , Hemocultura/normas , Coleta de Amostras Sanguíneas/instrumentação , Coleta de Amostras Sanguíneas/normas , Serviço Hospitalar de Educação , Reações Falso-Negativas , Pessoal de Saúde , Unidades Hospitalares , Humanos , Laboratórios Hospitalares , Recursos Humanos de Enfermagem no Hospital , Assistência ao Paciente , Sensibilidade e Especificidade
18.
J Immunol Methods ; 464: 114-118, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30343098

RESUMO

BACKGROUND: The use of anticoagulants may influence the composition of blood cells and interfere with plasma levels of IL-1ra when unprocessed EDTA blood samples are stored for long periods of time. METHODS: Blood was drawn into EDTA and heparinized blood collection tubes from 11 HIV-1 negative men participating in the Multicenter AIDS Cohort Study (MACS) and 4 healthy volunteers. The blood was processed according to the experiments detailed in the method and after incubation; supernatants were collected and stored at -70 °C until batch testing using IL-1ra ELISA. RESULTS: There was no difference between the levels of IL-1ra in EDTA blood collected into plastic and glass tubes (p = .911). There were significant increases from baseline levels of IL-1ra (p ≤ .05) after 24 h incubation for diluted whole blood and PBMC supernatants but not for granulocytes supernatants. CONCLUSION: EDTA as an anticoagulant influences the blood concentrations of IL-1ra in unprocessed blood. Thus, EDTA blood is not a suitable specimen for measurement of IL-1ra. Other types of anticoagulated blood should be processed within one hour of draw whenever measuring plasma levels of IL-1ra.


Assuntos
Anticoagulantes/efeitos adversos , Coleta de Amostras Sanguíneas , Quelantes de Cálcio/efeitos adversos , Equipamentos Descartáveis , Ácido Edético/efeitos adversos , Granulócitos/efeitos dos fármacos , Proteína Antagonista do Receptor de Interleucina 1/sangue , Leucócitos Mononucleares/efeitos dos fármacos , Biomarcadores/sangue , Coleta de Amostras Sanguíneas/efeitos adversos , Coleta de Amostras Sanguíneas/instrumentação , Feminino , Granulócitos/imunologia , Humanos , Leucócitos Mononucleares/imunologia , Masculino , Gravidez
19.
Bioanalysis ; 11(1): 13-20, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30461295

RESUMO

AIM: Volumetric absorptive microsampling (VAM) is being increasingly applied in nonclinical pharmacokinetic studies. Although there are published results for VAM use in small molecule pharmacokinetics (PK) studies, there is limited data on the utility of VAM for protein therapeutics. RESULTS: We describe the use of Mitra® microsampler for blood sampling, ELISA quantitation and PK analysis of two marketed therapeutic monoclonal antibodies administered to rat. Results generated for these monoclonal antibodies using Mitra® were compared with both serum and whole blood sampling methods in the same study. CONCLUSION: The low relative standard deviation among the three sets of PK data suggest that Mitra® microsampler could be useful in early nonclinical PK studies for protein therapeutics where reduction and refinement of animal use is desirable.


Assuntos
Coleta de Amostras Sanguíneas/métodos , Daclizumabe/farmacocinética , Trastuzumab/farmacocinética , Animais , Coleta de Amostras Sanguíneas/instrumentação , Calibragem , Daclizumabe/administração & dosagem , Daclizumabe/sangue , Injeções Intravenosas , Injeções Subcutâneas , Masculino , Ratos Wistar , Trastuzumab/administração & dosagem , Trastuzumab/sangue
20.
Ann Clin Biochem ; 56(2): 219-227, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30373393

RESUMO

BACKGROUND: Specimen contamination that goes unnoticed can have many adverse consequences for patients including inappropriate investigations or treatment decisions based on erroneous results. Little is known about UK laboratory practices relating to specimen contamination; therefore, this national survey aimed to gather valuable baseline data. METHODS: An electronic survey consisting of 26 questions was designed to obtain key information relating to specimen contamination including its frequency, how it is identified by laboratories and actions taken in event of confirmed contamination. The survey was circulated to Heads of Departments of all NHS laboratories in the UK. RESULTS: Fifty-two responses (15%) were received from 353 laboratories surveyed. Recording and extracting specimen contamination data from laboratory IT systems appear to be a challenge for many laboratories. There is potentially a lack of awareness of correct order of draw for venous blood collection which is a factor known to contribute to contamination. There is wide variation in contamination rates (EDTA, citrate and drip arm), and the methods laboratories use to identify it which often rely on professional judgement. Similarly, there is little consensus among senior laboratory professionals on how best to report results on contaminated samples, and record events in risk management systems. CONCLUSIONS: There is a need for greater consensus on laboratories' approach to specimen contamination, particularly around mechanisms to identify and monitor it, and follow up actions. We make several recommendations to facilitate improvements it this area; however, there is a need to develop consensus guidelines which can aid both clinicians and laboratories.


Assuntos
Coleta de Amostras Sanguíneas/estatística & dados numéricos , Inquéritos e Questionários , Artefatos , Coleta de Amostras Sanguíneas/instrumentação , Ácido Cítrico/sangue , Ácido Edético/sangue , Reações Falso-Positivas , Humanos , Gestão de Riscos , Reino Unido
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA