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3.
BMC Plant Biol ; 21(1): 385, 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34416864

RESUMO

Research in the past decade has demonstrated that a single reference genome is not representative of a species' diversity. MaizeGDB introduces a pan-genomic approach to hosting genomic data, leveraging the large number of diverse maize genomes and their associated datasets to quickly and efficiently connect genomes, gene models, expression, epigenome, sequence variation, structural variation, transposable elements, and diversity data across genomes so that researchers can easily track the structural and functional differences of a locus and its orthologs across maize. We believe our framework is unique and provides a template for any genomic database poised to host large-scale pan-genomic data.


Assuntos
Confiabilidade dos Dados , Coleta de Dados/métodos , Bases de Dados como Assunto , Genoma de Planta , Genômica , Zea mays/genética , Variação Genética
4.
Surg Clin North Am ; 101(4): 693-701, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34242610

RESUMO

Continued advancement has forced medical education to accept new ways in which to incorporate technology into its curriculum. As a result, technology has become a cornerstone to all levels of the medical education. This article compiles and discusses various avenues in which technology serves and betters education, ranging from administrative databases to cloud-based storage. Overall, technology can serve various educational purposes, including compilation, circulation, and integration of educational materials. The modalities discussed within this article, while numerous and adaptable, are a small portion of what the technological world has to offer.


Assuntos
Educação de Pós-Graduação em Medicina/métodos , Tecnologia Educacional/métodos , Cirurgia Geral/educação , Coleta de Dados/métodos , Educação à Distância/métodos , Educação à Distância/organização & administração , Educação de Pós-Graduação em Medicina/organização & administração , Tecnologia Educacional/organização & administração , Humanos , Armazenamento e Recuperação da Informação/métodos , América do Norte , Software , Comunicação por Videoconferência
5.
BMC Infect Dis ; 21(1): 596, 2021 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-34157990

RESUMO

BACKGROUND: Measles outbreaks are prevalent throughout sub-Saharan Africa despite the preventive measures like vaccination that target under five-year-old children and health systems strengthening efforts like prioritizing the supply chain for supplies. Measles immunization coverage for Kasese district and Bugoye HC III in 2018 was 72 and 69%, respectively. This coverage has been very low and always marked red in the Red categorization (below the national target/poor performing) on the national league table indicators. The aim of this study was to assess the scope of the 2018-2019 measles outbreak and the associated risk factors among children aged 0-60 months in Bugoye sub-county, Kasese district, western Uganda. METHODS: We conducted a retrospective unmatched case-control study among children aged 0-60 months with measles (cases) who had either a clinical presentation or a laboratory confirmation (IgM positivity) presenting at Bugoye Health Centre III (BHC) or in the surrounding communities between December 2018 and October 2019.. Caregivers of the controls (whose children did not have measles) were selected at the time of data collection in July 2020. A modified CDC case investigation form was used in data collection. Quantitative data was collected and analyzed using Microsoft excel and STATA version 13. The children's immunization cards and health registers at BHC were reviewed to ascertain the immunization status of the children before the outbreak. RESULTS: An extended measles outbreak occurred in Bugoye, Uganda occured between December 2018 and October 2019. All 34 facility-based measles cases were documented to have had maculopapular rash, conjunctivitis, and cough. Also, the majority had fever (97%), coryza (94.1%), lymphadenopathy (76.5%), arthralgias (73.5%) and Koplik Spots (91.2%) as documented in the clinical registers. Similar symptoms were reported among 36 community-based cases. Getting infected even after immunized, low measles vaccination coverage were identified as the principal risk factors for this outbreak. CONCLUSION: Measles is still a significant problem. This study showed that this outbreak was associated with under-vaccination. Implementing a second routine dose of measles-rubella vaccine would not only increase the number of children with at least one dose but also boost the immunity of those who had the first dose.


Assuntos
Surtos de Doenças , Sarampo/epidemiologia , Estudos de Casos e Controles , Pré-Escolar , Coleta de Dados/métodos , Feminino , Humanos , Imunização , Lactente , Recém-Nascido , Masculino , Fatores de Risco , Uganda/epidemiologia , Cobertura Vacinal
7.
PLoS One ; 16(6): e0253154, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34143823

RESUMO

BACKGROUND: Cohorts of hospitalized COVID-19 patients have been studied in several countries since the beginning of the pandemic. So far, there is no complete survey of older patients in a German district that includes both outpatients and inpatients. In this retrospective observational cohort study, we aimed to investigate risk factors, mortality, and functional outcomes of all patients with COVID-19 aged 70 and older living in the district of Tübingen in the southwest of Germany. METHODS: We retrospectively analysed all 256 patients who tested positive for SARS-CoV-2 in one of the earliest affected German districts during the first wave of the disease from February to April 2020. To ensure inclusion of all infected patients, we analysed reported data from the public health department as well as the results of a comprehensive screening intervention in all nursing homes of the district (n = 1169). Furthermore, we examined clinical data of all hospitalized patients with COVID-19 (n = 109). RESULTS: The all-cause mortality was 18%. Screening in nursing homes showed a point-prevalence of 4.6%. 39% of residents showed no COVID-specific symptoms according to the official definition at that time. The most important predictors of mortality were the need for inpatient treatment (odds ratio (OR): 3.95 [95%-confidence interval (CI): 2.00-7.86], p<0.001) and care needs before infection (non-hospitalized patients: OR: 3.79 [95%-CI: 1.01-14.27], p = 0.037, hospitalized patients: OR: 2.89 [95%-CI 1.21-6.92], p = 0.015). Newly emerged care needs were a relevant complication of COVID-19: 27% of previously self-sufficient patients who survived the disease were not able to return to their home environment after discharge from the hospital. CONCLUSION: Our findings demonstrate the importance of a differentiated view of risk groups and long-term effects within the older population. These findings should be included in the political and social debate during the ongoing pandemic to evaluate the true effect of COVID-19 on healthcare systems and individual functional status.


Assuntos
COVID-19/prevenção & controle , Hospitalização/estatística & dados numéricos , Pacientes Internados/estatística & dados numéricos , Casas de Saúde/estatística & dados numéricos , Pacientes Ambulatoriais/estatística & dados numéricos , SARS-CoV-2/isolamento & purificação , Idoso , Idoso de 80 Anos ou mais , COVID-19/epidemiologia , COVID-19/virologia , Coleta de Dados/métodos , Coleta de Dados/estatística & dados numéricos , Feminino , Alemanha/epidemiologia , Humanos , Masculino , Pandemias , Prevalência , Estudos Retrospectivos , Fatores de Risco , SARS-CoV-2/fisiologia
9.
PLoS Negl Trop Dis ; 15(5): e0009351, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33983937

RESUMO

Locally tailored interventions for neglected tropical diseases (NTDs) are becoming increasingly important for ensuring that the World Health Organization (WHO) goals for control and elimination are reached. Mathematical models, such as those developed by the NTD Modelling Consortium, are able to offer recommendations on interventions but remain constrained by the data currently available. Data collection for NTDs needs to be strengthened as better data are required to indirectly inform transmission in an area. Addressing specific data needs will improve our modelling recommendations, enabling more accurate tailoring of interventions and assessment of their progress. In this collection, we discuss the data needs for several NTDs, specifically gambiense human African trypanosomiasis, lymphatic filariasis, onchocerciasis, schistosomiasis, soil-transmitted helminths (STH), trachoma, and visceral leishmaniasis. Similarities in the data needs for these NTDs highlight the potential for integration across these diseases and where possible, a wider spectrum of diseases.


Assuntos
Controle de Doenças Transmissíveis/métodos , Coleta de Dados/métodos , Doenças Negligenciadas/epidemiologia , Doenças Negligenciadas/prevenção & controle , Filariose Linfática/epidemiologia , Filariose Linfática/transmissão , Humanos , Leishmaniose Visceral/epidemiologia , Leishmaniose Visceral/transmissão , Modelos Teóricos , Oncocercose/epidemiologia , Oncocercose/transmissão , Esquistossomose/epidemiologia , Esquistossomose/transmissão , Solo/parasitologia , Tracoma/epidemiologia , Tracoma/transmissão , Medicina Tropical/métodos , Tripanossomíase Africana/epidemiologia , Tripanossomíase Africana/transmissão
10.
Glob Health Sci Pract ; 9(2): 355-364, 2021 06 30.
Artigo em Inglês | MEDLINE | ID: mdl-34038385

RESUMO

Global misinformation and information overload have characterized the coronavirus disease (COVID-19) pandemic. Rumors are unverified pieces of information spreading online or person-to-person that reduce trust in health authorities and create barriers to protective practices. Risk communication and community engagement can increase transparency, build trust, and stop the spread of rumors. Building on previous work on Ebola and Zika viruses using Global Health Security Agenda systems strengthening support, the U.S. Agency for International Development-funded Breakthrough ACTION project developed a process and technology for systematically collecting, analyzing, and addressing COVID-19 rumors in real-time in Côte d'Ivoire. Rumors were submitted through community-based contributors and collected from callers to the national hotlines and then processed on a cloud-hosted database built on the open-source software District Health Information System 2 (DHIS2). Hotline teleoperators and data managers coded rumors in near-real-time according to behavioral theory frameworks within DHIS2 and visualized the findings on custom dashboards. The analysis and response were done in full collaboration with the Government of Côte d'Ivoire and implementing partners to ensure a timely and coordinated response. The system captured both widespread rumors consistent with misinformation in other settings, such as suspicions about case counts and the belief that masks were deliberately contaminated, as well as very localized beliefs related to specific influencers. The qualitative findings provided rapid insights on circulating beliefs, enabling risk communicators to nuance and tailor messaging around COVID-19.


Assuntos
COVID-19 , Comunicação , Conhecimentos, Atitudes e Prática em Saúde , Gestão da Informação/métodos , Pandemias , Características de Residência , Confiança , Controle de Doenças Transmissíveis , Costa do Marfim , Coleta de Dados/métodos , Bases de Dados Factuais , Governo , Linhas Diretas , Humanos , Cooperação Internacional , Internet , SARS-CoV-2
11.
Methods Mol Biol ; 2305: 291-299, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33950395

RESUMO

Cryo electron microscopy (cryo-EM) has become a method of choice in structural biology to analyze isolated complexes and cellular structures. This implies adequate imaging of the specimen and advanced image-processing methods to obtain high-resolution 3D reconstructions. The use of a Volta phase plate in cryo-EM drastically increases the image contrast while being able to record images at high acceleration voltage and close to focus, i.e., at conditions where high-resolution information is best preserved. During image processing, higher contrast images can be aligned and classified better than lower quality ones resulting in increased data quality and the need for less data. Here, we give step-by-step guidelines on how to set up high-quality VPP cryo-EM single particle data collections, as exemplified by human ribosome data acquired during a one-day data collection session. Further, we describe specific technical details in image processing that differ from conventional single particle cryo-EM data analysis.


Assuntos
Microscopia Crioeletrônica/métodos , Coleta de Dados/métodos , Ribossomos/ultraestrutura , Microscopia Crioeletrônica/instrumentação , Confiabilidade dos Dados , Humanos , Imageamento Tridimensional/métodos , Biologia Molecular/métodos , Ribossomos/química , Imagem Individual de Molécula
12.
Methods Mol Biol ; 2305: 323-342, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33950397

RESUMO

Microcrystal Electron Diffraction (MicroED) is the newest cryo-electron microscopy (cryo-EM) method, with over 70 protein, peptide, and several small organic molecule structures already determined. In MicroED, micro- or nanocrystalline samples in solution are deposited on electron microscopy grids and examined in a cryo-electron microscope, ideally under cryogenic conditions. Continuous rotation diffraction data are collected and then processed using conventional X-ray crystallography programs. The protocol outlined here details how to obtain and identify the nanocrystals, how to set up the microscope for screening and for MicroED data collection, and how to collect and process data to complete high-resolution structures. For well-behaving crystals with high-resolution diffraction in cryo-EM, the entire process can be achieved in less than an hour.


Assuntos
Microscopia Crioeletrônica/métodos , Cristalografia por Raios X/métodos , Microscopia Eletrônica de Transmissão/métodos , Peptídeos/química , Proteínas/química , Coleta de Dados/métodos , Elétrons , Modelos Moleculares , Biologia Molecular/métodos , Nanopartículas , Conformação Proteica , Fluxo de Trabalho
14.
Eur J Cardiovasc Nurs ; 20(4): 392-396, 2021 05 22.
Artigo em Inglês | MEDLINE | ID: mdl-33893797

RESUMO

Face-to-face interviews have long been the norm for conducting qualitative interviews in healthcare research. However, the Covid-19 pandemic has accelerated the need to explore alternative methods. This, along with the swift digitalization of healthcare, has led to video, telephone, and online interactions becoming increasingly used. The use of new techniques to carry out interviews through video, telephone, and online applications all come with benefits and drawbacks. In this article, three ways of collecting data through qualitative interviews are described and their uses exemplified through a project investigating the impact of a transition program for adolescents with congenital heart disease.


Assuntos
COVID-19 , Coleta de Dados/métodos , Correio Eletrônico , Cardiopatias Congênitas/diagnóstico , Cardiopatias Congênitas/enfermagem , Redes Sociais Online , Telefone , Gravação em Vídeo , Adolescente , Enfermagem Cardiovascular/métodos , Feminino , Humanos , Masculino , Pandemias , Pesquisa Qualitativa , SARS-CoV-2
15.
Washington; Organización Panamericana de la Salud; abr. 14, 2021. 10 p.
Não convencional em Inglês, Espanhol | LILACS | ID: biblio-1178358

RESUMO

Esta herramienta está diseñada para realizar una evaluación rápida de las instituciones prestadoras de servicios de salud y de otras relacionadas con la salud y analiza su capacidad para recopilar datos críticos y de alta calidad en la respuesta a la pandemia de COVID-19. La herramienta evalúa el compromiso institucional con la pertinencia y la calidad de los datos utilizados para la toma de decisiones a todos los niveles. Además, facilita la autoevaluación para redefinir las prioridades de la pandemia; identifica la brecha de capacidad de los sistemas de datos internacionales, nacionales y subnacionales, y evalúa la capacidad para proporcionar datos fiables y constructivos.


This tool is designed for rapid assessment of health and health-related institutions and evaluates their capacity to gather critical, high-quality data in the COVID-19 pandemic response. The tool assesses institutional commitment to the timeliness and quality of data used for decision-making at all levels. It facilitates self-assessment to redefine pandemic priorities and, identifies the capacity gap within international, national, and subnational data systems, and assesses the ability to provide reliable and constructive data.


Assuntos
Pneumonia Viral/epidemiologia , Coleta de Dados/métodos , Interpretação Estatística de Dados , Infecções por Coronavirus/epidemiologia , Pandemias/estatística & dados numéricos , Sistemas de Informação em Saúde/estatística & dados numéricos
17.
Acad Med ; 96(7): 1050-1056, 2021 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-33735133

RESUMO

PURPOSE: Social and behavioral determinants of health (SBDH) are important factors that affect the health of individuals but are not routinely captured in a structured and systematic manner in electronic health records (EHRs). The purpose of this study is to generate recommendations for systematic implementation of SBDH data collection in EHRs through (1) reviewing SBDH conceptual and theoretical frameworks and (2) eliciting stakeholder perspectives on barriers to and facilitators of using SBDH information in the EHR and priorities for data collection. METHOD: The authors reviewed SBDH frameworks to identify key social and behavioral variables and conducted focus groups and interviews with 17 clinicians and researchers at Johns Hopkins Health System between March and May 2018. Transcripts were coded and common themes were extracted to understand the barriers to and facilitators of accessing SBDH information. RESULTS: The authors found that although the frameworks agreed that SBDH affect health outcomes, the lack of model consensus complicates the development of specific recommendations for the prioritization of SBDH data collection. Study participants recognized the importance of SBDH information and individual health and agreed that patient-reported information should be captured, but clinicians and researchers cited different priorities for which variables are most important. For the few SBDH variables that are captured, participants reported that data were often incomplete, unclear, or inconsistent, affecting both researcher and clinician responses to SBDH barriers to health. CONCLUSIONS: Health systems need to identify and prioritize the systematic implementation of collection of a high-impact but limited list of SBDH variables in the EHR. These variables should affect care and be amenable to change and collection should be integrated into clinical workflows. Improved data collection of SBDH variables can lead to a better understanding of how SBDH affect health outcomes and ways to better address underlying health disparities that need urgent action.


Assuntos
Coleta de Dados/métodos , Registros Eletrônicos de Saúde/estatística & dados numéricos , Avaliação de Resultados em Cuidados de Saúde/estatística & dados numéricos , Determinantes Sociais da Saúde/estatística & dados numéricos , Confiabilidade dos Dados , Atenção à Saúde/normas , Feminino , Grupos Focais/métodos , Disparidades em Assistência à Saúde/legislação & jurisprudência , Humanos , Entrevistas como Assunto/métodos , Masculino , Avaliação de Resultados em Cuidados de Saúde/tendências , Participação dos Interessados , Fluxo de Trabalho
18.
J Ayub Med Coll Abbottabad ; 33(1): 145-149, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33774972

RESUMO

BACKGROUND: Operative notes are the most important account of a surgical procedure containing all details that may have been encountered during the surgery. Summary: Below par quality of these notes remains a challenge for the healthcare system and guidelines from the Royal College of Surgeons should be made use of to ensure efficient recording. Operative notes need to be legible with clear and concise instructions for the nursing staff for postoperative care. Key messages: Relevant details of surgery, additional procedures and complications should be thoroughly documented as well to avoid losses in terms of financial remuneration and for medicolegal purposes. Residents in training should be taught about their importance and inclusion in surgical curriculum should be sought.


Assuntos
Coleta de Dados , Registros Médicos/normas , Procedimentos Cirúrgicos Operatórios/normas , Redação/normas , Coleta de Dados/métodos , Coleta de Dados/normas , Humanos , Guias de Prática Clínica como Assunto , Cirurgiões , Procedimentos Cirúrgicos Operatórios/métodos
20.
J Med Internet Res ; 23(3): e23984, 2021 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-33720028

RESUMO

The collection of data from a personal digital device to characterize current health conditions and behaviors that determine how an individual's health will evolve has been called digital phenotyping. In this paper, we describe the development of and early experiences with a comprehensive digital phenotyping platform: Health Outcomes through Positive Engagement and Self-Empowerment (HOPES). HOPES is based on the open-source Beiwe platform but adds a wider range of data collection, including the integration of wearable devices and further sensor collection from smartphones. Requirements were partly derived from a concurrent clinical trial for schizophrenia that required the development of significant capabilities in HOPES for security, privacy, ease of use, and scalability, based on a careful combination of public cloud and on-premises operation. We describe new data pipelines to clean, process, present, and analyze data. This includes a set of dashboards customized to the needs of research study operations and clinical care. A test use case for HOPES was described by analyzing the digital behavior of 22 participants during the SARS-CoV-2 pandemic.


Assuntos
Coleta de Dados/métodos , Aprendizado de Máquina , Dispositivos Eletrônicos Vestíveis , Computadores de Mão , Humanos , Aplicativos Móveis , Fenótipo , Projetos de Pesquisa , Esquizofrenia/diagnóstico , Esquizofrenia/terapia , Smartphone
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