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1.
Zhonghua Wai Ke Za Zhi ; 57(12): 927-933, 2019 Dec 01.
Artigo em Chinês | MEDLINE | ID: mdl-31826598

RESUMO

Objectives: To examine the value of multi-slice spiral CT angiography (MSCTA) in the analysis of anatomical variation and structural classification of right colon vessels. Methods: From August 2015 to August 2017, 198 patients (96 of whom underwent laparoscopic radical resection of right colon cancer) at Department of General surgery of Peking University First Hospital were retrospectively collected, and the results of abdominal enhanced CT scan were collected and three-dimensional reconstruction of blood vessels was performed. There were 104 males and 94 females. The age was 64(27) years (M(Q(R)), range: 19 to 87 years). Right gastroepiploic vein, anterior superior pancreaticoduodenal vein, right colonic vein (RCV), superior right colonic vein, ileocolon artery or vein (ICA or ICV), middle colon artery or vein (MCA or MCV) and Henle trunk were observed and recorded respectively. The anatomical relationship between the positions of blood vessels, the length of Henle trunk and surgical trunk were measured. Results: ICV and ICA were the most constant anatomic structures. The ICV/ICA of all patients came directly from SMV/SMA, 36.9% (73/198) ICV going in front of SMV and 63.1% (125/198) behind SMV. 72.2% (143/198) of the patients had RCV imported into Henle trunk and the rest into SMV. Middle colonic vein (MCV) could be observed in 81.3% (161/198) of the cases. 81.4% (131/161) of MCV were imported into SMV, 16.8% (27/161) into Henle trunk, 1.2% (2/161) into the first jejunal vein and 0.6% (1/161) into the splenic vein. Henle trunk was divided into 4 types, among which the occurrence probability of gastric node and pancreatic trunk was the highest. The dry length of Henle trunk was (0.82±0.39) cm (range: 0.37 to 1.68 cm). The length of surgical trunk was (2.54±0.83) cm (range: 1.57 to 3.95 cm). Accuracy of MSCTA results was 96.9%(93/96). Conclusions: Anatomical variation of blood vessels in the right colon is common. Abdominal CT angiography can accurately determine the anatomical structure of the blood vessels in the right colon.


Assuntos
Colo/irrigação sanguínea , Colo/diagnóstico por imagem , Neoplasias do Colo/irrigação sanguínea , Neoplasias do Colo/diagnóstico por imagem , Adulto , Idoso , Idoso de 80 Anos ou mais , Colectomia/métodos , Colo/cirurgia , Neoplasias do Colo/cirurgia , Angiografia por Tomografia Computadorizada , Feminino , Humanos , Laparoscopia , Masculino , Pessoa de Meia-Idade , Cuidados Pré-Operatórios , Estudos Retrospectivos , Circulação Esplâncnica , Tomografia Computadorizada Espiral , Adulto Jovem
2.
BMC Infect Dis ; 19(1): 992, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31752711

RESUMO

BACKGROUND: Histoplasmosis is one of the invasive fungal infections and presents with symptoms mainly in the lungs. Disseminated histoplasmosis (DH) is rare and its lesions in the gastrointestinal tract are even uncommon. The concomitant involvement of the upper and lower gastrointestinal tract has never been described in the immunocompetent individuals. CASE PRESENTATION: A 44-year-old immunocompetent Chinese man presented with fever, hepatosplenomegaly, fungal esophagitis and protuberant lesions with central depression and erosion along the mucous membrane of the colon. The patient was diagnosed as disseminated histoplasmosis by gastrointestinal endoscopy. CONCLUSIONS: Histoplasmosis should be taken caution in patients with fever and hepatosplenomegaly. Actions should be taken to avoid its disseminated infection associated high mortality.


Assuntos
Histoplasma/isolamento & purificação , Histoplasmose/diagnóstico , Adulto , Colo/diagnóstico por imagem , Colo/patologia , Endoscopia Gastrointestinal , Histoplasma/classificação , Histoplasma/genética , Histoplasmose/diagnóstico por imagem , Histoplasmose/imunologia , Histoplasmose/microbiologia , Humanos , Hospedeiro Imunocomprometido , Masculino
4.
Turkiye Parazitol Derg ; 43(3): 149-151, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31502806

RESUMO

The aim of the present study was to present a case with secondary hydatid cysts in both uterus and colon. The patient was a 71-year-old female living in Hakkari, Turkey. She was admitted to the Van Yuzuncu Yil University Faculty of Medicine Medical Center with complaints of chronic abdominal and pelvic pain, and swelling in the abdomen. First, the sagittal T2 weighted magnetic rezonance imaging (MR) showed a type-3 cyst hydatid with daughter vesicles located at the posterior of uterus. Later, MR revealed a type-2 cystic lesion with detached membrane adhered to the anterior wall of colon and it was reported to be associated with abdomen. When the previous liver surgery history of the patient was kept in mind, the new finding was suggestive of a secondary cystic hydatid . In conclusion, it is possible to diagnose secondary cystic echinococcosis in patients with a history of primary cyst surgery in liver or any other organ by combining the symptoms and imaging findings.


Assuntos
Colo/parasitologia , Equinococose/diagnóstico , Útero/parasitologia , Idoso , Animais , Colo/diagnóstico por imagem , Equinococose/diagnóstico por imagem , Equinococose/cirurgia , Equinococose Hepática/complicações , Equinococose Hepática/cirurgia , Echinococcus , Feminino , Humanos , Fígado/parasitologia , Fígado/cirurgia , Imagem por Ressonância Magnética , Recidiva , Turquia , Útero/diagnóstico por imagem
6.
Anal Bioanal Chem ; 411(26): 6969-6981, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31418050

RESUMO

Fourier transform infrared (FTIR) spectroscopic imaging of colon biopsy tissues in transmission combined with machine learning for the classification of different stages of colon malignancy was carried out in this study. Two different approaches, an optical and a computational one, were applied for the elimination of the scattering background during the measurements and compared with the results of the machine learning model without correction for the scattering. Several different data processing pathways were implemented in order to obtain a high accuracy of the prediction model. This study demonstrates, for the first time, that C-H stretching and amide I bands are of little to no significance in the classification of the colon malignancy, based on the Gini importance values by random forest (RF). The best prediction outcome is found when supervised RF classification was carried out in the fingerprint region of the spectral data between 1500 and 1000 cm-1 (excluding the contribution of amide I and II bands). An overall prediction accuracy higher than 90% is achieved through the RF. The results also show that dysplastic and hyperplastic tissues are well distinguished. This leads to the insight that the important differences between hyperplastic and dysplastic colon tissues lie within the fingerprint region of FTIR spectra. In this study, computational correction performed better than optical correction, but the findings show that the disease states of colon biopsies can be distinguished effectively without elimination of Mie scattering effect. Graphical abstract.


Assuntos
Colo/diagnóstico por imagem , Neoplasias do Colo/diagnóstico por imagem , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Colo/química , Neoplasias do Colo/química , Aprendizado de Máquina , Prognóstico
7.
Int J Mol Sci ; 20(14)2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-31295965

RESUMO

Noninvasive Raman imaging of non-fixed and unstained human colon tissues based on vibrational properties of noncancerous and cancerous samples can effectively enable the differentiation between noncancerous and tumor tissues. This work aimed to evaluate the biochemical characteristics of colon cancer and the clinical merits of multivariate Raman image and spectroscopy analysis. Tissue samples were collected during routine surgery. The non-fixed, fresh samples were used to prepare micrometer sections from the tumor mass and the tissue from the safety margins outside of the tumor mass. Adjacent sections were used for typical histological analysis. We have found that the chemical composition identified by Raman spectroscopy of the cancerous and the noncancerous colon samples is sufficiently different to distinguish pathologically changed tissue from noncancerous tissue. We present a detailed analysis of Raman spectra for the human noncancerous and cancerous colon tissue. The multivariate analysis of the intensities of lipids/proteins/carotenoids Raman peaks shows that these classes of compounds can statistically divide analyzed samples into noncancerous and pathological groups, reaffirming that Raman imaging is a powerful technique for the histochemical analysis of human tissues. Raman biomarkers based on ratios for lipids/proteins/carotenoids content were found to be the most useful biomarkers in spectroscopic diagnostics.


Assuntos
Colo/diagnóstico por imagem , Colo/metabolismo , Neoplasias do Colo/etiologia , Neoplasias do Colo/metabolismo , Processamento de Imagem Assistida por Computador , Análise Espectral Raman , Biomarcadores , Neoplasias do Colo/diagnóstico , Humanos , Processamento de Imagem Assistida por Computador/métodos , Estadiamento de Neoplasias , Análise Espectral Raman/métodos
8.
J Clin Ultrasound ; 47(8): 453-460, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31343081

RESUMO

PURPOSE: The aim is to investigate whether baseline contrast-enhanced ultrasound (CEUS) correlates with indices of activity in Crohn's disease (CD) and can predict response to medical treatment. METHODS: In this prospective study, symptomatic CD patients underwent baseline CEUS performed with Definity using both bolus and infusion methods. Time-intensity curves (TIC), peak intensity (PI), and area under curve (AUC) from a region of interest over the diseased bowel were calculated for both bolus and infusion acquisitions. We used Mann-Whitney U test for continuous and chi-square/two-tailed Fisher's exact test for categorical variable comparison and Spearman's correlation coefficient to correlate clinical score and CEUS kinetic parameters. RESULTS: Twenty-one patients (9 men, 12 women, median age 32 years) were accrued. Fifteen patients had clinically active disease defined as Harvey-Bradshaw Index (HBI) score ≥5. Median values of baseline CEUS parameters PI (bolus: 26 vs 8.86; P = .023 and perfusion: 7.6 vs 3.2; P = .009) and AUC (bolus: 769 vs 248.8; P = .036 and perfusion: 188.9 vs 73.9; P = .012) differed significantly in patients with active vs inactive disease. Nine patients with active disease underwent escalated or new treatment. Five were nonresponders. Responders had higher median values of baseline parameters (PI, bolus: 35 vs 18.8; P = .556, and perfusion: 7.6 vs 3.9; P = 190), (AUC, bolus: 1473.9 vs 314; P = .111, and perfusion: 154.7 vs 74.4, P = .286). CONCLUSIONS: CEUS kinetic parameters correlate with clinical and laboratory indices and are significantly higher in patients with active disease. The responders had higher CEUS kinetic parameters than nonresponders that did not reach statistical significance in our small cohort.


Assuntos
Colo/irrigação sanguínea , Meios de Contraste/farmacologia , Doença de Crohn/diagnóstico , Fluxo Sanguíneo Regional/fisiologia , Ultrassonografia Doppler em Cores/métodos , Adulto , Colo/diagnóstico por imagem , Doença de Crohn/fisiopatologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Adulto Jovem
9.
Radiol Med ; 124(12): 1185-1198, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31302848

RESUMO

Clostridium difficile infection (CDI) is a severe and potentially deadly infectious colitis whose incidence is dramatically increasing in the last decades, with more virulent strains. CDI should be suspected in case of unexplained diarrhea and abdominal pain in patients with a recent history of antibiotic use and healthcare exposures; diagnosis is based on a combination of clinical and laboratory findings with demonstration of C. difficile toxins by stool test. The advantages of contrast-enhanced computed tomography (CECT) are the noninvasiveness and the ability to evaluate both the colonic wall and the adjacent soft tissues. Considerable overlap exists between the CECT findings of CDI and those of colitis of other origins, such as typhlitis, ischemic colitis, graft-versus-host disease, radiation colitis and inflammatory bowel diseases; however, some features may help distinguish between these conditions. This paper provides a comprehensive overview of the imaging features of Clostridium difficile colitis and its mimics, with a view to assist the radiologist in reaching the correct diagnosis.


Assuntos
Clostridium difficile , Colo/diagnóstico por imagem , Meios de Contraste , Enterocolite Pseudomembranosa/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Colite/diagnóstico por imagem , Colo/irrigação sanguínea , Colo/patologia , Colo/efeitos da radiação , Diagnóstico Diferencial , Doença Enxerto-Hospedeiro/diagnóstico por imagem , Humanos , Isquemia/diagnóstico por imagem , Lesões por Radiação/diagnóstico por imagem , Tiflite/diagnóstico por imagem
11.
BMC Gastroenterol ; 19(1): 114, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31262270

RESUMO

BACKGROUND: Microcirculatory disturbance is an important factor in the pathogenesis of Inflammatory Bowel Disease (IBD) but there have been few studies in this field. Confocal Laser Endomicroscopy (CLE) has been used over the last 10 years and has made it possible to explore the changes in microcirculation of the colonic mucosa. METHODS: We retrospectively selected patients who underwent probe-based Confocal Laser Endomicroscopy (pCLE) between 2014 and 2016. There were 7 patients with ulcerative colitis (UC) in clinical remission and 7 healthy subjects included in this study; all the UC patients' medical data were reviewed. For each patient, three segments of the colon were examined using pCLE including the ascending, transverse/descending and sigmoid colon. In each segment, the representative pCLE images of the three sites were selected for analysis. Four indicators, including Mean Vessel Diameter (MVD), Diameter Standard Deviation (DSD), Functional Capillary Density-long (FCDL) and Functional Capillary Density-area (FCDA), were measured with a specially designed detection software algorithm. The four indicators were compared between UC patients and healthy subjects. According to the different blood flow patterns, three types of distribution were established: the Around (A), Cobweb (C) and Deficiency (D) type. The relationships between the recurrence and blood flow patterns of UC patients were analyzed. RESULTS: MVD, DSD, FCDL and FCDA were 10.62 ± 0.56 µm, 2.23 ± 0.26, 0.030 ± 0.019 µm and 0.289 ± 0.030 for the healthy subjects and 11.06 ± 1.10 µm, 2.68 ± 0.29, 0.026 ± 0.005 µm and 0.272 ± 0.034 for the UC patients, respectively. Compared with healthy subjects, DSD was significantly increased and FCDA was significantly decreased (P < 0.01 for both). There was no difference in MVD and FCDL between UC patients and healthy subjects. The type A and type C blood flows were observed in healthy subjects (66.67 and 33.33%, respectively) while type C appears more in UC patients (71.3%) and type D blood flow could only be found in UC patients (14.29%) P < 0.01. UC patients who showed Type D blood flow had a shorter recurrence interval. CONCLUSIONS: Some local mucosal capillary density in UC patients was decreased, particularly in the inflammation-affected segment. The three mucosal blood flow patterns can be used as an indicator of mucosal healing.


Assuntos
Colite Ulcerativa/fisiopatologia , Colo/irrigação sanguínea , Colonoscopia/métodos , Mucosa Intestinal/irrigação sanguínea , Microcirculação , Adulto , Colite Ulcerativa/diagnóstico por imagem , Colo/diagnóstico por imagem , Colo Sigmoide/irrigação sanguínea , Colo Sigmoide/diagnóstico por imagem , Feminino , Humanos , Masculino , Microscopia Confocal , Pessoa de Meia-Idade , Fluxo Sanguíneo Regional , Indução de Remissão , Estudos Retrospectivos
12.
Am J Forensic Med Pathol ; 40(4): 376-380, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31306168

RESUMO

Barotrauma-associated perforation of the colon is not common and usually occurs due to the passage of compressed air through the anus. Most of the cases are accidental and done for fun often at the victim's workplace. Therefore, it is necessary that the workers should be made aware of the dangers of the equipment they regularly use at their workplace. Here, we describe one such case where a rice mill worker died when compressed air through an air pump pipe entered his rectum. His chief complaint was abdominal pain and breathing difficulty. Computed tomography scan of the abdomen and thorax showed pneumoperitoneum, pneumomediastinum, and soft tissue emphysema. There was a complete tear in the rectosigmoid junction of the colon. The mucosa was deeply hemorrhagic and congested. Histopathology of this segment showed hemorrhagic necrosis of the mucosa.


Assuntos
Colo/lesões , Ar Comprimido/efeitos adversos , Perfuração Intestinal/etiologia , Exposição Ocupacional/efeitos adversos , Colo/diagnóstico por imagem , Colo/patologia , Evolução Fatal , Hemorragia Gastrointestinal/diagnóstico por imagem , Hemorragia Gastrointestinal/patologia , Humanos , Mucosa Intestinal/patologia , Perfuração Intestinal/diagnóstico por imagem , Perfuração Intestinal/patologia , Masculino , Enfisema Mediastínico/diagnóstico por imagem , Necrose , Peritonite/etiologia , Pneumoperitônio/diagnóstico por imagem , Tomografia Computadorizada por Raios X
13.
Pharmacology ; 104(1-2): 51-56, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31067545

RESUMO

Mesalazine is the gold standard drug for treatment of ulcerative colitis (UC). Here, we describe 4 cases of familial adenomatous polyposis (FAP) patients with UC that showed reduction of intestinal polyp diameter by mesalazine treatment. Of note, the effects of mesalazine on the development of intestinal polyps in FAP patients have not been reported, and we further investigated whether the short-term use of high-dose mesalazine (4 g/day) has harmful effects on FAP patients or not. The authors found that the treatment showed slightly adverse events in FAP patients. However, mesalazine tended to reduce the number of colon polyps in male subjects with FAP. This report provides basic information for planning a double-blind, randomized, clinical trial that aims to show mesalazine's potential to suppress intestinal polyp development in FAP.


Assuntos
Polipose Adenomatosa do Colo/tratamento farmacológico , Anti-Inflamatórios não Esteroides/administração & dosagem , Colite Ulcerativa/tratamento farmacológico , Mesalamina/administração & dosagem , Polipose Adenomatosa do Colo/complicações , Polipose Adenomatosa do Colo/patologia , Administração Oral , Adulto , Anti-Inflamatórios não Esteroides/efeitos adversos , Colite Ulcerativa/complicações , Colite Ulcerativa/patologia , Colo/diagnóstico por imagem , Colo/efeitos dos fármacos , Colo/patologia , Colonoscopia , Relação Dose-Resposta a Droga , Seguimentos , Humanos , Mucosa Intestinal/diagnóstico por imagem , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/patologia , Masculino , Mesalamina/efeitos adversos , Projetos Piloto , Resultado do Tratamento , Adulto Jovem
14.
Jpn J Radiol ; 37(8): 590-596, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31127495

RESUMO

PURPOSE: Cinematic rendering (CR) is a new technique for visualizing volumetric three-dimensional data. The purpose of this study was to investigate the added value of CR to conventional computed tomography (CT) in the diagnosis and evaluation of ulcerative colitis (UC). MATERIALS AND METHODS: We retrospectively evaluated the CT data of 48 patients (33 men, 15 women; mean age, 44.35 years) with a definitive diagnosis of UC. All patients underwent conventional CT and CR, and had colonoscopy results. Two radiologists independently reviewed the conventional CT images first without and then with CR. Then, the imaging value of CR was evaluated by both radiologists together. The readers were blinded to the disease extent. The diagnostic performance of CR for both readers was assessed by receiver-operating characteristic (ROC) curve analysis. RESULTS: There were 23 cases of mild to moderate UC and 25 cases of severe UC, which were divided into two groups. Both readers showed improved diagnostic performance with the addition of CR (the area under the ROC curve improved from 0.676 to 0.804, P = 0.0255, and from 0.679 to 0.826, P = 0.0049, for readers 1 and 2, respectively). Full view of the lesion and contrast enhancement was not significantly different between the two groups (P > 0.05). Increased mesenteric vascularity and the comb sign on CR were more clearly observed in the severe group (P < 0.05). CONCLUSION: Adding CR to conventional CT improved the diagnostic performance of evaluating the extent of UC.


Assuntos
Colite Ulcerativa/diagnóstico por imagem , Interpretação de Imagem Assistida por Computador/métodos , Imagem Tridimensional/métodos , Tomografia Computadorizada por Raios X/métodos , Adolescente , Adulto , Idoso , Colo/diagnóstico por imagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Curva ROC , Estudos Retrospectivos , Adulto Jovem
15.
Ann R Coll Surg Engl ; 101(5): e125-e127, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31042432

RESUMO

Visual inspection of the content of the hernia sac is a crucial step of the emergency repair of strangulated inguinal hernias, to rule out the presence of bowel ischaemia which would need resection. Occasionally the content of the hernia sac reduces spontaneously into the abdomen after the induction of general anaesthesia and cannot be assessed. We present a case where hernioscopy t(i.e. laparoscopy through the hernia sac) has been used to confirm the ischaemia of the strangulated bowel and perform a hybrid laparohernioscopic right colectomy with intracorporeal anastomosis without the need for a formal laparotomy. Hernioscopy is an easy and reliable method to assess the viability of the content of the hernia sac in strangulated hernias that have spontaneously reduced before it could be visually inspected. It can also be associated with a laparoscopic access in a hybrid laparohernioscopic technique to perform complex procedures.


Assuntos
Colectomia/métodos , Colo/irrigação sanguínea , Hérnia Inguinal/cirurgia , Herniorrafia/métodos , Isquemia/cirurgia , Laparoscopia/métodos , Idoso , Colo/diagnóstico por imagem , Colo/cirurgia , Hérnia Inguinal/complicações , Hérnia Inguinal/diagnóstico por imagem , Humanos , Isquemia/diagnóstico por imagem , Isquemia/etiologia , Masculino
16.
World J Gastroenterol ; 25(15): 1899-1906, 2019 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-31057303

RESUMO

BACKGROUND: Cytomegalovirus (CMV) remains a critical complication after solid-organ transplantation. The CMV antigenemia (AG) test is useful for monitoring CMV infection. Although the AG-positivity rate in CMV gastroenteritis is known to be low at onset, almost all cases become positive during the disease course. We treated a patient with transverse colon perforation due to AG-negative CMV gastroenteritis, following a living donor liver transplantation (LDLT). CASE SUMMARY: The patient was a 52-year-old woman with decompensated liver cirrhosis as a result of autoimmune hepatitis who underwent a blood-type compatible LDLT with her second son as the donor. On day 20 after surgery, upper and lower gastrointestinal endoscopy (GE) revealed multiple gastric ulcers and transverse colon ulcers. The biopsy tissue immunostaining confirmed a diagnosis of CMV gastroenteritis. On day 28 after surgery, an abdominal computed tomography revealed transverse colon perforation, and simple lavage and drainage were performed along with an urgent ileostomy. Although the repeated remission and aggravation of CMV gastroenteritis and acute cellular rejection made the control of immunosuppression difficult, the upper GE eventually revealed an improvement in the gastric ulcers, and the biopsy samples were negative for CMV. The CMV-AG test remained negative, therefore, we had to evaluate the status of the CMV infection on the basis of the clinical symptoms and GE. CONCLUSION: This case report suggests a monitoring method that could be useful for AG-negative CMV gastroenteritis after a solid-organ transplantation.


Assuntos
Doenças do Colo/diagnóstico , Infecções por Citomegalovirus/complicações , Gastroenterite/complicações , Perfuração Intestinal/diagnóstico , Transplante de Fígado/efeitos adversos , Antígenos Virais/sangue , Antígenos Virais/imunologia , Colo/diagnóstico por imagem , Colo/virologia , Doenças do Colo/etiologia , Citomegalovirus/imunologia , Citomegalovirus/isolamento & purificação , Infecções por Citomegalovirus/sangue , Infecções por Citomegalovirus/imunologia , Infecções por Citomegalovirus/virologia , Doença Hepática Terminal/imunologia , Doença Hepática Terminal/patologia , Doença Hepática Terminal/cirurgia , Endoscopia Gastrointestinal , Feminino , Gastroenterite/sangue , Gastroenterite/imunologia , Gastroenterite/virologia , Hepatite Autoimune/imunologia , Hepatite Autoimune/patologia , Hepatite Autoimune/cirurgia , Humanos , Perfuração Intestinal/etiologia , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios X
17.
J Surg Res ; 242: 145-150, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31077946

RESUMO

INTRODUCTION: Claudins are tight-junction proteins, which maintain an epithelial barrier in normal colon cells. Overexpression of Claudin-1 has been implicated for development of colon cancer. We postulated that Claudin-1 may be a useful target in near-infrared imaging and fluorescence-guided surgery. METHODS: We conjugated Claudin-1 antibody to LI-COR IR800DyeCW (Claudin-1-IRDye800CW). Western blotting of 9 human colon cancer cell lysates was performed. Animal imaging was performed with the LI-COR Pearl Trilogy Fluorescence Imaging System. A dose-response study was carried out with subcutaneous LS174T colon cancer cell line models. Increasing doses of Claudin-1-IRDye800CW via tail vein injection were administered to three groups of mice. Two groups of mice were used as controls (antibody alone, and dye alone). In vivo imaging was performed at 24, 48, and 72 h after administration of the conjugated dye. Orthotopic implantation of patient-derived tumors and cell lines was performed and peritoneal carcinomatosis models were created. After tumor growth, mice were administered Claudin-1-IRDye800CW and imaged in vivo 48 h later. The mice were euthanized and laparotomy was performed to assess internal organs and toxicity. RESULTS: Western blotting revealed that all colon cancer cell lysates expressed varying amounts of Claudin-1. All tumors demonstrated strong and specific fluorescence labeling at 800 nm, even with the lowest dose of 12.5 µg of Claudin-1-IRDye800CW. CONCLUSIONS: Claudin-1 is a useful target for near-infrared antibody-based imaging for visualization of colorectal tumors for future use in fluorescence-guided surgery.


Assuntos
Claudina-1/imunologia , Neoplasias do Colo/diagnóstico por imagem , Corantes Fluorescentes/administração & dosagem , Imunoconjugados/administração & dosagem , Neoplasias Peritoneais/diagnóstico por imagem , Animais , Anticorpos Monoclonais/administração & dosagem , Anticorpos Monoclonais/imunologia , Benzenossulfonatos/administração & dosagem , Linhagem Celular Tumoral , Colo/diagnóstico por imagem , Colo/patologia , Neoplasias do Colo/patologia , Neoplasias do Colo/cirurgia , Relação Dose-Resposta a Droga , Humanos , Imunoconjugados/imunologia , Indóis/administração & dosagem , Injeções Intravenosas , Masculino , Camundongos , Camundongos Nus , Neoplasias Peritoneais/cirurgia , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Cirurgia Assistida por Computador/métodos , Ensaios Antitumorais Modelo de Xenoenxerto
19.
J Comput Assist Tomogr ; 43(3): 475-484, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31082955

RESUMO

Three-dimensional (3D) visualizations of computed tomography (CT) data have found use in the display of complex anatomy and pathology. A new method of 3D CT image creation known as cinematic rendering (CR) makes use of a global lighting model to produce photorealistic images. Cinematic rendering images contain high levels of detail with shadowing and depth that are not available from traditional 3D CT techniques. As yet, the role of CR in evaluating colonic pathology has not been investigated. However, given the breadth of pathologic processes that affect the colon, including inflammatory bowel disease, diverticulitis, neoplastic conditions, herniation, and gastrointestinal bleeding, we undertook a survey of recent cases at our institution to demonstrate colon pathology as visualized with CR. The following review discusses the role of 3D CT visualizations for colonic pathology with an emphasis on CR example images.


Assuntos
Colo/patologia , Tomografia Computadorizada por Raios X/métodos , Colo/diagnóstico por imagem , Feminino , Humanos , Imagem Tridimensional , Masculino , Interpretação de Imagem Radiográfica Assistida por Computador/métodos
20.
BMC Cancer ; 19(1): 428, 2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31072353

RESUMO

BACKGROUND: Colorectal cancer remains the second leading cause of cancer death in the United States, and increased risk in patients with ulcerative colitis (a subset of inflammatory bowel disease) has motivated studies into early markers of dysplasia. The development of clinically translatable multiphoton imaging systems has allowed for the potential of in vivo label-free imaging of epithelial crypt structures via autofluorescence and/or second harmonic generation (SHG). SHG has been used to investigate collagen structures in various types of cancer, though the changes that colorectal epithelial collagen structures undergo during tumor development, specifically colitis-associated tumors, have not been fully investigated. METHODS: This study used two murine models, using A/J mice, one for spontaneous carcinoma and one for colitis-associated carcinoma, to investigate and quantify SHG image features that could potentially inform future study designs of endoscopic multiphoton imaging systems. The spontaneous tumor model comprised a series of six weekly injections of azoxymethane (AOM model). The colitis-associated tumor model comprised a single injection of AOM, followed by cycles of drinking water with dissolved dextran sodium sulfate salt (AOM-DSS model). SHG images of freshly resected murine colon were acquired with a multiphoton imaging system, and image features, such as crypt size, shape and distribution, were quantified using an automated algorithm. RESULTS: The comparison of quantified features of crypt morphology demonstrated the ability of our quantitative image feature algorithms to detect differences between spontaneous (AOM model) and colitis-associated (AOM-DSS model) murine colorectal tissue specimens. There were statistically significant differences in the mean and standard deviation of nearest neighbor (distance between crypts) and circularity between the Control cohort, AOM and AOM-DSS cohorts. We also saw significance between AOM and AOM-DSS cohorts when calculating nearest neighbor in images acquired at fixed depths. CONCLUSION: The results provide insight into the ability of SHG imaging to yield relevant data about the crypt microstructure in colorectal epithelium, specifically the potential to distinguish between spontaneous and colitis-associated murine models using quantification of crypt shape and distribution, informing future design of translational multiphoton imaging systems and protocols.


Assuntos
Colite/patologia , Colo/patologia , Neoplasias do Colo/diagnóstico por imagem , Mucosa Intestinal/patologia , Microscopia de Geração do Segundo Harmônico , Animais , Colite/induzido quimicamente , Colite/diagnóstico por imagem , Colo/diagnóstico por imagem , Neoplasias do Colo/patologia , Sulfato de Dextrana/toxicidade , Modelos Animais de Doenças , Progressão da Doença , Humanos , Mucosa Intestinal/diagnóstico por imagem , Camundongos
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