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1.
Mol Immunol ; 101: 294-302, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30032071

RESUMO

Atopic asthma, which is characterized by the chronic inflammation and morbidity of airways, is a disease of great complexity, and multiple genetic and environmental factors are involved in its etiology. In the first genome-wide association study (GWAS) conducted in Brazil for asthma, a positive association was found between atopic asthma and a variant (rs1999071), which is located between the DAD1 and OXA1L genes, although neither gene has previously been reported to be associated with asthma or allergies. The DAD1 gene is involved in the regulation of programmed cell death, and OXA1L is involved in biogenesis and mitochondrial oxidative phosphorylation. This study aimed to evaluate how polymorphisms in DAD1 and OXA1L are associated with asthma and markers of atopy in individuals from the Salvador cohort of the SCAALA (Social Change Asthma and Allergy in Latin America) program. The DNA of 1220 individuals was genotyped using the Illumina 2.5 Human Omni Bead chip. Logistic regression analyses were performed with PLINK 1.9 software to verify the association between DAD1 and OXA1L polymorphisms and asthma and atopic markers, adjusted for sex, age, helminth infections and ancestry markers, using an additive model. The DAD1 and OXA1L genes were associated with some of the evaluated phenotypes, such as asthma, skin prick test (SPT), specific IgE for aeroallergens, and Th1/Th2-type cytokine production. Using qPCR, as well as in silico gene expression analysis, we have demonstrated that some of the polymorphisms in both genes are able to affect their respective gene expression levels. In addition, DAD1 was over-expressed in asthmatic patients when compared with controls. Thus, our findings demonstrate that variants in both the DAD1 and OXA1L genes may affect atopy and asthma in a Latin American population with a high prevalence of asthma.


Assuntos
Proteínas Reguladoras de Apoptose/genética , Asma/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Predisposição Genética para Doença , Hipersensibilidade Imediata/genética , Proteínas de Membrana/genética , Proteínas Mitocondriais/genética , Proteínas Nucleares/genética , Polimorfismo de Nucleotídeo Único/genética , Asma/sangue , Brasil , Estudos de Casos e Controles , Criança , Pré-Escolar , Simulação por Computador , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Feminino , Regulação da Expressão Gênica , Estudo de Associação Genômica Ampla , Humanos , Hipersensibilidade Imediata/sangue , Desequilíbrio de Ligação/genética , Masculino , Proteínas Mitocondriais/sangue , Modelos Biológicos , Proteínas Nucleares/sangue , Fatores de Risco
2.
J Biomed Opt ; 23(5): 1-9, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29766685

RESUMO

Functional near-infrared spectroscopy (fNIRS) is a noninvasive method for measuring in vivo both hemodynamic and mitochondrial metabolic activities in brain cortical structures. Although the test-retest reliability of the hemodynamic measures, such as reflected by oxygenated (HbO2), deoxygenated (HHb) hemoglobin, and the tissue oxygenation index (TOI), has been previously reported to be good to excellent, the reliability of the metabolic signal indexed by oxidized cytochrome-c-oxidase (oxCCO) has not been reported. The present test-retest study compared the reliability of the metabolic and hemodynamic signals in 10 healthy participants undergoing hypo- and hypercapnia challenges. The primary reliability measure was the intraclass correlation coefficient (ICC). Results of both hypo- and hypercapnia showed that the oxCCO signal (ICC = 0.876 / 0.757) had robust reliability comparable with that of the HbO2 (ICC = 0.841 / 0.801), HHb (ICC = 0.804 / 0.571), and TOI (ICC = 0.574 / 0.614) signals. These findings show that the oxCCO signal can be assessed by fNIRS with comparable reliability to the hemodynamic measures. We discuss the results in light of current interest in a mitochondrial metabolic marker derived from fNIRS.


Assuntos
Encéfalo , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Processamento de Sinais Assistido por Computador , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Adulto , Encéfalo/irrigação sanguínea , Encéfalo/diagnóstico por imagem , Encéfalo/enzimologia , Feminino , Hemoglobinas/análise , Humanos , Hipercapnia , Hipocapnia , Masculino , Oxirredução , Oxiemoglobinas/análise , Reprodutibilidade dos Testes
3.
Enferm Infecc Microbiol Clin ; 36(9): 539-543, 2018 11.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-29054538

RESUMO

OBJECTIVE: The comparison on mitochondrial function between severe septic patients and healthy control subjects according to mitochondrial deoxyribonucleic acid (mtDNA) haplogroup has not been previously reported; and this was the objective of the current study. METHODS: Prospective, multicenter, observational study. We obtained blood samples from 198 severe septic patients at days 1, 4 and 8 of severe sepsis diagnosis and from 96 sex- and age-matched healthy controls to determine mtDNA haplogroup and platelet respiratory complex IV (CIV) specific activity. The endpoint of the study was 30-day mortality. RESULTS: We included 198 severe septic patients (38 with mtDNA haplogroup JT and 160 with mtDNA haplogroup non-JT) and 96 healthy control subjects (16 with mtDNA haplogroup JT and 80 with mtDNA haplogroup non-JT). We have no found statistically significant differences in platelet CIV specific activity between healthy controls and survivor severe septic patients with mtDNA haplogroup JT at days 1, 4 and 8 of severe sepsis diagnosis; and the remaining severe septic patients showed lower platelet CIV specific activity than healthy controls with the same mtDNA haplogroup. CONCLUSIONS: The new finding of our study was that survivor severe septic patients and healthy controls with mtDNA haplogroup JT showed no different platelet Civ specific activity.


Assuntos
DNA Mitocondrial/genética , Haplótipos , Mitocôndrias/fisiologia , Sepse/fisiopatologia , Adulto , Idoso , DNA Mitocondrial/sangue , DNA Mitocondrial/classificação , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Complexo IV da Cadeia de Transporte de Elétrons/genética , Feminino , Predisposição Genética para Doença , Humanos , Masculino , Pessoa de Meia-Idade , Fosforilação Oxidativa , Prognóstico , Estudos Prospectivos , Sepse/sangue , Sepse/genética , Sepse/mortalidade , Sobreviventes
4.
Circ J ; 81(6): 879-887, 2017 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-28302943

RESUMO

BACKGROUND: Maintaining cerebral oxygen delivery and metabolism during cardiac arrest (CA) through resuscitation is essential to improve the survival rate while avoiding brain injury. The effect of CA and cardiopulmonary resuscitation (CPR) on cerebral and muscle oxygen delivery and metabolism is not clearly quantified.Methods and Results:A novel hyperspectral near-infrared spectroscopy (hNIRS) technique was developed and evaluated to measure cerebral oxygen delivery and aerobic metabolism during ventricular fibrillation (VF) CA and CPR in 14 pigs. The hNIRS parameters were measured simultaneously on the dura and skull to investigate the validity of non-invasive hNIRS measurements. In addition, we compared the hNIRS data collected simultaneously on the brain and muscle. Following VF induction, oxygenated hemoglobin (HbO2) declined with a 9.9 s delay and then cytochrome-c-oxidase (Cyt-ox) decreased on average 4.4 s later (P<0.05). CPR improved cerebral metabolism, which was reflected by an average 0.4 µmol/L increase in Cyt-ox, but had no significant effect on HbO2, deoxygenated hemoglobin (HHb) and tissue oxygen saturation (tSO2). Cyt-ox had greater correlation with HHb than HbO2. Muscle metabolism during VF and CPR was significantly different from that of the brain. The total hemoglobin concentration (in the brain only) increased after ~200 s of untreated CA, which is most likely driven by cerebral autoregulation through vasodilation. CONCLUSIONS: Overall, hNIRS showed consistent measurements of hemodynamics and metabolism during CA and CPR.


Assuntos
Reanimação Cardiopulmonar , Circulação Cerebrovascular , Oxigênio/sangue , Espectrofotometria Infravermelho , Vasodilatação , Fibrilação Ventricular , Animais , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Feminino , Parada Cardíaca/sangue , Parada Cardíaca/fisiopatologia , Parada Cardíaca/terapia , Hemoglobinas/metabolismo , Suínos , Fibrilação Ventricular/sangue , Fibrilação Ventricular/fisiopatologia , Fibrilação Ventricular/terapia
5.
J Clin Neurosci ; 30: 31-38, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27262871

RESUMO

Recent studies have observed the central role of mitochondrial dysfunction in severe traumatic brain injury (sTBI). One hundred and seven sTBI patients (18-65years old, presenting within 8hours of injury) were randomised for a placebo controlled phase II trial of progesterone with or without hypothermia. We serially analysed blood mitochondrial enzymes (Complex I [C1], Complex IV [C4] and pyruvate dehydrogenase complex [PDH]) using a dipstick assay at admission and 7days later for 37 patients, irrespective of assigned group. Favorable Glasgow Outcome Scale (GOS) at 1year was associated with admission C1 levels above 0.19µg, admission C4 levels above 0.19µg and day 7 C1 levels above 0.17µg, all per 25µl of blood. Unfavorable GOS at 1year was associated with admission serum PDH levels above 0.23µg/25µl of blood. Survivors at 1year had significantly higher admission serum C1 levels above 0.19µg/25µl and day 7 C1 levels above 0.17µg/25µl. To our knowledge this is the first clinical trial associating blood mitochondrial enzymes with long-term outcome in sTBI. Serial monitoring and optimisation of blood C1, C4 and PDH levels could aid in prognostication and potentially guide in using mitochondrial targeted therapies. Blood mitochondrial enzymatic assay might suggest global reduction-oxidation status.


Assuntos
Biomarcadores/sangue , Lesões Encefálicas Traumáticas/sangue , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Complexo I de Transporte de Elétrons/sangue , Complexo Piruvato Desidrogenase/sangue , Adulto , Lesões Encefálicas Traumáticas/terapia , Ensaios Enzimáticos , Feminino , Escala de Coma de Glasgow , Escala de Resultado de Glasgow , Humanos , Hipotermia Induzida/métodos , Masculino , Pessoa de Meia-Idade , Progesterona/uso terapêutico
6.
J Biomed Opt ; 21(9): 091307, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27170072

RESUMO

Near-infrared spectroscopy (NIRS) measurements of cytochrome-c-oxidase (CCO) have the potential to yield crucial information about cerebral metabolism at the patient bedside. Developments in instrumentation and the analytical methods used to resolve changes in CCO have led to many clinical applications of the measurement since its first demonstration in 1977 by Jöbsis. There is a substantial literature of work on measures of CCO in animal and in vitro studies; however, this review focuses on translational studies. Almost 40 years from the advent of the first measurement of CCO using NIRS, this signal continues to hold significant interest in our understanding of the human brain in health and disease. We discuss methodologies for obtaining NIRS measurements of CCO in the clinic and review studies in neonates and adults.


Assuntos
Encéfalo , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Imagem Óptica/métodos , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Encéfalo/irrigação sanguínea , Encéfalo/diagnóstico por imagem , Encéfalo/enzimologia , Humanos , Processamento de Imagem Assistida por Computador , Processamento de Sinais Assistido por Computador
7.
Adv Exp Med Biol ; 911: 45-52, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26987334

RESUMO

Peripheral blood mononuclear cells (PBMC) represent an easily available population of cells for the studies on remote effects of lung cancer. NADH dehydrogenase (ubiquinone) Fe-S protein-1 (Ndufs1), a marker of mitochondrial complex I, and mitochondrially encoded cytochrome c oxidase 1 (MTCO1), a marker of complex IV, may participate in cognitive decline during the course of lung cancer. In this study, Ndufs1 and MTCO1 expression in PBMC was evaluated by means of ELISA in 80 lung cancer patients. Mini-Mental State Examination (MMSE) were conducted Trail Making Tests (TMT-A and TMT-B) at baseline and after the 6 months' follow-up. Autoantibodies were identified by means of indirect immunofluorescence and line blot. We found that enhanced levels of Ndufs1 in PBMC were related to impaired cognitive performance; TMT-A of 13.6 ± 3.1 s and TMT-B of 162.5 ± 46.4 s compared with 8.6 ± 4.5 s (p = 0.003) and 124.8 ± 51.8 s (p < 0.05), respectively, in the case of low Ndufs-1 levels. The Ndufs1 expression at baseline was associated with MMSE - τb (Kendall's tau-b) = -0.31; p = 0.024; TMT-A - τb = 0.30; p = 0.001), and TMT-B - τb = 0.199; p = 0.012) after the 6 months' follow-up. Higher MTCO1 expression was accompanied by worse TMT-A results than in case of inhibited MTCO1; 11.1 ± 5.8 s vs. 8.5 ± 4.1 s; respectively; p = 0.048. MTCO1 expression was correlated with TMT-A results (τb = 0.17; p = 0.034) at baseline. We conclude that stimulation of PBMC mitochondrial function in lung cancer patients is associated with cognitive impairment. Mitochondrial dysfunction in PBMC may reflect cytotoxicity responsible for neurological deficits.


Assuntos
Biomarcadores Tumorais/sangue , Transtornos Cognitivos/diagnóstico , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Neoplasias Pulmonares/complicações , NADH Desidrogenase/sangue , Adenocarcinoma/complicações , Adenocarcinoma/patologia , Adenocarcinoma/psicologia , Carcinoma de Células Grandes/complicações , Carcinoma de Células Grandes/patologia , Carcinoma de Células Grandes/psicologia , Carcinoma Pulmonar de Células não Pequenas/complicações , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma Pulmonar de Células não Pequenas/psicologia , Carcinoma de Células Escamosas/complicações , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/psicologia , Transtornos Cognitivos/sangue , Transtornos Cognitivos/etiologia , Transtornos Cognitivos/psicologia , Ensaio de Imunoadsorção Enzimática , Feminino , Seguimentos , Humanos , Leucócitos Mononucleares/metabolismo , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/psicologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Testes Neuropsicológicos , Prognóstico , Desempenho Psicomotor , Carcinoma de Pequenas Células do Pulmão/complicações , Carcinoma de Pequenas Células do Pulmão/patologia , Carcinoma de Pequenas Células do Pulmão/psicologia , Teste de Sequência Alfanumérica
8.
Med Pr ; 66(4): 539-48, 2015.
Artigo em Polonês | MEDLINE | ID: mdl-26536970

RESUMO

BACKGROUND: The aim of the study was to evaluate serum levels of the target enzyme for H2S toxicity--cytochrome c oxidase (COX) and enzymes involved in the synthesis of H2S--cystathionine ß-synthase (CBS) and cystathionine γ-lyase (CSE) in copper mine miners. MATERIAL AND METHODS: The initial and basic study was conducted respectively in 237 and 88 miners, working in 2 mining shafts: I--no H2S emissions recorded in the last 10 years (study group A) and II--H2S emissions occurred (study group B). A medical examination was performed and 10 ml of blood was collected from miners immediately after exiting the mine. RESULTS: There were no clinical or biochemical changes typical for H2S toxicity. Sulfhemoglobine was undetectable and there were no changes in the red-ox system. However, in group B, regulatory changes were found; a tendency to higher concentration of CBS and CSE, a higher activity of angiotensin converting enzyme (ACE) compared to group A (p<0.05) and a linear relationship between ACE and CSE (r=0.6927; p<0.001). It has been shown that cigarette smoking decreases COX (p<0.05), however, in miners working in shaft II, the decreased level of COX may result also from the presence of H2S in the gaseous emissions. CONCLUSIONS: COX concentration can be a sensitive indicator of exposure to H2S. The measurements of blood H2S concentrations carried out in workplaces should explain the cause of the changes observed in the COX, CBS and CSE activity.


Assuntos
Cobre/efeitos adversos , Cistationina beta-Sintase/sangue , Cistationina gama-Liase/sangue , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Sulfeto de Hidrogênio/metabolismo , Mineração , Doenças Profissionais/metabolismo , Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Mineradores , Doenças Profissionais/diagnóstico , Exposição Ocupacional/efeitos adversos , Polônia , Estudos Retrospectivos
9.
Crit Care ; 18(3): R136, 2014 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-24981786

RESUMO

INTRODUCTION: In a previous study with 96 septic patients, we found that circulating platelets in 6-months surviving septic patients showed higher activity and quantity of cytochrome c oxidase (COX) normalized by citrate synthase (CS) activity at moment of severe sepsis diagnosis than non-surviving septic patients. The objective of this study was to estimate whether COX specific activity during the first week predicts 1-month sepsis survival in a larger cohort of patients. METHODS: Using a prospective, multicenter, observational study carried out in six Spanish intensive care units with 198 severe septic patients, we determined COX activity per proteins (COXact/Prot) in circulating platelets at day 1, 4 and 8 of the severe sepsis diagnosis. Endpoints were 1-month and 6-months mortality. RESULTS: Survivor patients (n = 130) showed higher COXact/Prot (P < 0.001) than non-survivors (n = 68) at day 1, 4 and 8 of severe sepsis diagnosis. More than a half of the 6-months survivor patients showed an increase in their COXact/Prot from day 1 to 8. However, most of the 1-month non-survivors exhibited a decrease in their COXact/Prot from day 1 to 8. Multiple logistic regression analyses showed that of platelet COXact/Prot > 0.30 mOD/min/mg at day 1 (P = 0.002), 4 (P = 0.006) and 8 (P = 0.02) was associated independently with 1-month mortality. Area under the curve of COXact/Prot at day 1, 4 and 8 to predict 30-day survival were 0.70 (95% CI = 0.63-0.76; P < 0.001), 0.71 (95% CI = 0.64-0.77; P < 0.001) and 0.71 (95% CI = 0.64-0.78; P < 0.001), respectively. CONCLUSIONS: The new findings of our study, to our knowledge the largest series reporting data about mitochondrial function during follow-up in septic patients, were that septic patients that survive 1-month have a higher platelet cytochrome oxidase activity at moment of sepsis diagnosis and during the first week than non-survivors, and that platelet cytochrome oxidase activity at moment of sepsis diagnosis and during the first week could be used as biomarker to predict the clinical outcome in septic patients.


Assuntos
Plaquetas/enzimologia , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Sepse/enzimologia , Biomarcadores/sangue , Humanos , Unidades de Terapia Intensiva , Mitocôndrias/enzimologia , Fosforilação Oxidativa , Prognóstico , Estudos Prospectivos , Sepse/diagnóstico , Sepse/mortalidade , Sobreviventes
10.
Radiats Biol Radioecol ; 52(6): 602-7, 2012.
Artigo em Russo | MEDLINE | ID: mdl-23516891

RESUMO

The influence of UV-light (240-390 nm) at the dozes of 151 and 755 J/m2 on the intensity of processes of the lipid peroxidation, activity of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), cytochrome c oxidase and the level of the energy supply of donors' blood lymphocytes in the absence and presence of autologous plasma was investigated. It was shown that during the incubation of native and UV-irradiated lymphocytes, autologous plasma reduces the intensity of lipid peroxidation, thus protecting cells from oxidative stress. As a result, the endocellular level of ATP is restored in UV-irradiated lymphocytes (during the daily incubation), which reflects the intensification of the adaptive ability of cells in the presence of autologous plasma.


Assuntos
Peroxidação de Lipídeos/efeitos da radiação , Linfócitos , Plasma/efeitos da radiação , Raios Ultravioleta , Células Cultivadas , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Humanos , L-Lactato Desidrogenase/sangue , Contagem de Linfócitos , Linfócitos/enzimologia , Linfócitos/efeitos da radiação , Estresse Oxidativo/efeitos da radiação , Succinato Desidrogenase/sangue
11.
Transfusion ; 52(5): 1024-30, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22098205

RESUMO

BACKGROUND: Intracellular adenosine triphosphate (ATP) levels decline significantly during storage of platelet (PLT) products, in part due to PLT degranulation. However, metabolic ATP stores also become depleted during storage through an unclear mechanism. Since both anaerobic glycolysis and oxidative phosphorylation are important for PLT ATP production, it is possible that the reduction in metabolic ATP reflects impaired oxidative phosphorylation. To assess this, we evaluated the kinetic activity and protein expression of cytochrome C oxidase (CcOX) in stored apheresis PLTs. STUDY DESIGN AND METHODS: Apheresis PLTs were collected and stored with agitation at 22 ± 2°C for 7 days. In vitro measurements of PLT metabolic state, function, and activation were performed on Days 0, 2, 4, and 7 of storage. Total PLT ATP content, steady-state CcOX kinetic activity, and protein immunoblotting for CcOX Subunits I and IV were also performed using isolated PLT mitochondria from simultaneously collected samples. RESULTS: Intra-PLT ATP and steady-state PLT CcOX activity declined significantly and in a progressive manner throughout storage while steady-state levels of CcOX I and IV protein remained unchanged. Time-dependent decline in CcOX activity correlated with progressive ATP depletion over time. CONCLUSION: During storage of apheresis PLTs for 7 days, the parallel decline in CcOX function and intra-PLT ATP suggests development of an acquired impairment in PLT oxidative phosphorylation associated with perturbed ATP homeostasis in stored PLTs.


Assuntos
Trifosfato de Adenosina/metabolismo , Plaquetas/metabolismo , Preservação de Sangue , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Plaquetoferese , Complexo IV da Cadeia de Transporte de Elétrons/fisiologia , Humanos
12.
Sleep Breath ; 16(2): 505-10, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21573911

RESUMO

OBJECTIVE: The objective of this study was to investigate the effects of chronic intermittent hypoxia (CIH) on genioglossal ultrastructure and mitochondrial function as well as the intervention role of adiponectin (Ad). METHODS: Forty-two Wistar rats were randomly divided into three groups with 14 rats in each. Rats in group A were kept breathing normal air, while rats in both groups B and C received the same CIH environment (a 2-min cycle, 1 min on, 1 min off with a nadir O(2) at 4-5%, 8 h/day for successive 5 weeks). However, rats in group C was given regular intravenous Ad injection (10 µg per time, twice a week for successive 5 weeks). A simultaneous intravenous injection of saline (0.5 ml per time) was carried in groups A and B. At the end of experiment, the genioglossal ultrastructure, the serum adiponectin levels, the mitochondrial membrane potential (ΔΨ(m)), and activities of respiratory chain complexes I and IV in mitochondrion of genioglossal cells were compared among groups. RESULTS: Serum Ad level was significantly lower in group B than that in group A (P < 0.01). In group B, there were genioglussal myofibril discontinuities, lysis of myofilament, edema of mitochondria, and disruption of cristae, vacuolus, and lysis of some mitochondria. These pathological changes were less significant in group C. The relative value of ΔΨ(m) was the lowest in group B but the highest in group A (P < 0.01), with group B in between. The concentrations of mitochondrial complexes I and IV in group B were the lowest but became higher and higher from group C to A, with a significant difference among groups (all P < 0.05). CONCLUSION: CIH could lead to hypoadiponectinemia, impaired genioglossal ultrastructure, and mitochondrial dysfunction. These changes could be improved by supplement of Ad.


Assuntos
Complexo IV da Cadeia de Transporte de Elétrons/sangue , Complexo I de Transporte de Elétrons/sangue , Hipóxia/patologia , Mitocôndrias Musculares/patologia , Língua/patologia , Adiponectina/sangue , Animais , Complexo I de Transporte de Elétrons/administração & dosagem , Metabolismo Energético/fisiologia , Citometria de Fluxo , Masculino , Potencial da Membrana Mitocondrial/fisiologia , Microscopia Eletrônica de Transmissão , Ratos , Ratos Wistar , Valores de Referência
13.
J Comp Physiol B ; 180(5): 707-14, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20135129

RESUMO

We examined the effect of exercise intensity and endurance training on plasma free fatty acid (FFA) kinetics and lipid metabolism in swimming muscles of reared sea trout. In both training groups [water current velocities 1 and 2 body lengths per second (bl s(-1))] the plasma level of FFAs decreased significantly (P < 0.001) compared to the control group. Similar significant (P < 0.01) post-exercise decrease was observed also in the lipase-esterase activity in the red muscle, but not in white. Moreover, in the group swimming with higher intensity a significantly higher (P < 0.05) lipase-esterase activity in the red muscle was found compared with the group on moderate exercise. As with cytochrome c oxidase activity, a significant elevation in the enzyme activity was also observed after training in the 1 bl s(-1) group in red and white muscle (P < 0.05 and P < 0.01, respectively). No changes were observed in beta hydroxyacyl CoA dehydrogenase activity. The lipid content was on average nine times higher in red compared to white muscle being 16.7, 21.1, and 24.9% in the red muscle of the control, 1 and 2 bl s(-1) groups, respectively, with a significant (P < 0.05) increase after training. We conclude that (1) unlike in mammals, plasma FFA kinetics and oxidation are not linearly related to exercise intensity in reared sea trout, (2) training enhances the capacity to uptake FFA from plasma, and (3) high intensity training shifts the proportion of energy derived from fat oxidation to carbohydrate-derived energy.


Assuntos
Metabolismo dos Lipídeos/fisiologia , Condicionamento Físico Animal , Natação/fisiologia , Truta/fisiologia , 3-Hidroxiacil-CoA Desidrogenases/sangue , Animais , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Ácidos Graxos não Esterificados/sangue , Feminino , Lipase/metabolismo , Masculino , Músculo Esquelético/fisiologia , Resistência Física/fisiologia
14.
Nutr Res ; 29(7): 494-502, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19700037

RESUMO

Sensitive blood biochemical markers of dietary copper status are not yet known. Rat models were used to investigate the response of severe copper deficiency in dams and pups by comparing abundance of several cuproproteins in erythrocytes, white blood cells, and platelets. The hypothesis tested was that copper deficiency would result in changes in abundance of cuproproteins in blood cells. Copper-deficient (CuD) Holtzman dams and pups had signs consistent with severe copper deficiency compared with copper-adequate controls including lower liver copper and hemoglobin levels and near total loss of plasma ceruloplasmin diamine oxidase activity. Copper-deficient erythrocytes had lower copper, zinc superoxide dismutase (SOD1) but higher copper metallochaperone for SOD1 (CCS) compared with copper-adequate, resulting in higher CCS/SOD1 levels. This ratio was more sensitive in CuD erythrocytes than CuD white cells and especially in CuD platelets. However, both white blood cells and platelets from CuD dams and pups had nearly nondetectable levels of cytochrome c oxidase subunit IV. Because isolation of relatively pure populations of erythrocytes and platelets is feasible, and reagents for immunoblot methods are available, determination of CCS/SOD1 and cytochrome c oxidase subunit IV protein levels may be useful to assess copper status of humans.


Assuntos
Amina Oxidase (contendo Cobre)/sangue , Ceruloplasmina/metabolismo , Cobre/deficiência , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Eritrócitos/metabolismo , Hemoglobinas/metabolismo , Superóxido Dismutase/sangue , Animais , Biomarcadores/sangue , Plaquetas/metabolismo , Proteínas Sanguíneas/metabolismo , Cobre/metabolismo , Ferro/metabolismo , Leucócitos/metabolismo , Fígado/metabolismo , Chaperonas Moleculares , Ratos , Ratos Sprague-Dawley , Zinco/sangue
16.
EMBO Rep ; 7(11): 1128-33, 2006 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17008930

RESUMO

Trypanosomes use RNA editing to produce most functional mitochondrial messenger RNA. Precise insertion and deletion of hundreds of uridines is necessary to make full-length cytochrome c oxidase III (COXIII) mRNA. We show that COXIII mRNA can be alternatively edited by a mechanism using an alternative guide RNA to make a stable mRNA. This alternatively edited mRNA is translated to produce a unique protein that fractionates with mitochondrial membranes and colocalizes with mitochondrial proteins in situ. Alternative RNA editing represents a previously unknown mechanism generating protein diversity and, as such, represents an important function for RNA editing.


Assuntos
Complexo IV da Cadeia de Transporte de Elétrons/sangue , Edição de RNA , RNA Mensageiro/metabolismo , RNA de Protozoário/metabolismo , Trypanosoma brucei brucei/enzimologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Complexo IV da Cadeia de Transporte de Elétrons/genética , Microscopia de Fluorescência , Dados de Sequência Molecular , RNA Guia/genética , RNA Mitocondrial , Homologia de Sequência de Aminoácidos , Trypanosoma brucei brucei/genética
18.
Toxicol Ind Health ; 22(1): 39-46, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16572710

RESUMO

This investigation gives detailed analysis of peripheral marker enzymes as well as neurobehavioral tests following chronic aluminium (Al) exposure (10 mg/kg b.w. for 12 weeks intragastrically). We observed a significant decrease in the levels of serum cholinesterase after toxicity. The enzymatic activity of cytochrome oxidase (CO), the terminal enzyme of the electron transport chain, was significantly diminished and that of glucose-6-phosphate dehydrogenase (G-6-PD) was significantly enhanced. Neuromuscular co-ordination was assessed using motor and memory function tests. Deficits were observed suggesting a probable model for chronic Al neurotoxicity.


Assuntos
Alumínio/toxicidade , Comportamento Animal/efeitos dos fármacos , Poluentes Ambientais/toxicidade , Síndromes Neurotóxicas/etiologia , Equilíbrio Postural/efeitos dos fármacos , Animais , Biomarcadores/sangue , Colinesterases/sangue , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Glucosefosfato Desidrogenase/sangue , Masculino , Síndromes Neurotóxicas/sangue , Síndromes Neurotóxicas/enzimologia , Ratos , Ratos Wistar , Testes de Toxicidade Crônica
19.
Resuscitation ; 68(1): 27-44, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16325319

RESUMO

Clinicians now realize the limitations of the physical examination in detecting compensated shock states, the severity of uncompensated states, and in determining the adequacy of resuscitation in order to prevent subsequent post-traumatic multisystem organ failure and death. A renewed interest has developed in interrogating the state of oxygen transport at the end-organ level in the trauma patient. Although used as a research tool and now clinically to monitor cerebral oxygenation during complex cardiovascular and neurosurgery, near infrared absorption spectroscopy (NIRS) is being more aggressively investigated and now marketed clinically as a noninvasive means to assess tissue oxygenation in the trauma patient at the end organ level. This paper will describe the principles of NIRS and the basis for its proposed use in the trauma patient to assess tissue oxygenation. This includes its known limitations, current controversies, and what will be needed in the future to make this technology a part of the initial and ongoing assessment of the trauma patient. The ultimate goal of such techniques is to prevent misassessment of patients and inadequate resuscitation, which are believed to be major initiators in the development of multisystem organ failure and death.


Assuntos
Choque Traumático/sangue , Espectroscopia de Luz Próxima ao Infravermelho , Ferimentos e Lesões/sangue , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Hemoglobinas/análise , Humanos , Mioglobina/sangue , Oxigênio/metabolismo , Consumo de Oxigênio , Ressuscitação , Choque Traumático/terapia , Espectroscopia de Luz Próxima ao Infravermelho/instrumentação , Espectroscopia de Luz Próxima ao Infravermelho/métodos
20.
J Thorac Cardiovasc Surg ; 130(3): 830-6, 2005 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16153936

RESUMO

OBJECTIVE: During repair of aortic coarctation through a left thoracotomy without cardiopulmonary bypass, clamping the proximal transverse aortic arch occludes antegrade flow to the left carotid and vertebral arteries. It is assumed that flow through the right carotid and vertebral arteries is adequate for cerebral perfusion. The study objective is to determine whether aortic occlusion impairs left hemispheric cerebral oxygen balance measured by near-infrared spectroscopy. METHODS: In 18 children having repair of aortic coarctation, we measured the maximum change and integral for hemoglobin D (difference of oxyhemoglobin and deoxyhemoglobin), total oxygenation index, and the redox state of cytochrome aa3. Thirteen subjects had recordings from the left hemisphere to test the hypothesis that aortic occlusion impairs left hemispheric oxygen balance. Five subjects had recordings from the right hemisphere for comparison. RESULTS: After aortic clamping, a significant decrease in hemoglobin D was observed in recordings from the left hemisphere compared with those from the right hemisphere (P = .03, maximum change in hemoglobin D). Total oxygenation index and cytochrome aa3 were generally preserved. There was an inverse linear relationship for the change in hemoglobin D during clamp application and after removal (Spearman rho = -0.74), with increased hemoglobin D after clamp removal in those subjects with the greatest decrease of hemoglobin D during arch occlusion. Linear regression analysis identified nitroprusside administration as significantly associated with a decrease in hemoglobin D (P < .001). CONCLUSIONS: Significant impairment in left hemispheric cerebral oxygen balance was identified during arch clamping. The neurodevelopmental significance of impaired cerebral oxygen balance detected by near-infrared spectroscopy during aortic coarctation repair remains to be elucidated.


Assuntos
Coartação Aórtica/cirurgia , Circulação Cerebrovascular , Complexo IV da Cadeia de Transporte de Elétrons/sangue , Oxigênio/metabolismo , Aorta Torácica , Encéfalo/metabolismo , Criança , Pré-Escolar , Constrição , Hemoglobinas Anormais/análise , Humanos , Lactente , Recém-Nascido , Monitorização Intraoperatória , Nitroprussiato/farmacologia , Oxigênio/sangue , Espectroscopia de Luz Próxima ao Infravermelho
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