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1.
J Chromatogr A ; 1609: 460452, 2020 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-31443970

RESUMO

Absolute quantification of B-vitamins in milk is becoming imperative to correlate its impact on child/human health. In today's world of changing food habits and environmental pollution, there is concern if milk is what we think it to be. In the present work, ultra-high performance liquid chromatography mass spectrometry/selected reaction monitoring (UPHPLC-MS/SRM) methods have been developed and validated for quantitative estimation of 21 different B-vitamins [B1-3, B2-3, B3-2, B5-1, B6-5, B8-1, B9-2 vitamins, total choline (betaine, choline and acetylcholine)] from a drop (50 µL) and B12 from 500 µL of milk. These two methods qualify all essential bio-analytical parameters (recovery >80%, accuracy <±15%, variation <±10%) and are highly reproducible. By using these developed methods, B-vitamins in different fresh milk samples from cow, goat, buffalo and pasteurized cow milk (each 10 and total n = 40) were analysed. Total choline is the highest (6.5-10.5 µg/mL) and vitamin B8 is the lowest (16.1-32.9 ng/mL) in all milk samples. Inverse correlation between vitamin B2 and B5 was observed in milk samples. The ratio of vitamin B5/B2 was checked in all milk samples, it is higher in cow (2.64), equal in goat (1.04) and lower in buffalo (0.42) milk. Total B-vitamin content in cow milk is higher (10.5 µg/mL) compared to other three (goat-7.2, buffalo-6.5, pasteurized-8.8 µg/mL). Vitamin B12 is higher in cow milk (3.6 ng/mL) compared to other two fresh milk samples. Different isomers for vitamin B6 were noticed in the fresh milk samples. The complete profile of water soluble vitamins and the ratio of two abundant B vitamins (B5/B2) in milk will be useful to check the nutritional quality and to differentiate the kind of animal milk.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Leite/química , Espectrometria de Massas em Tandem/métodos , Complexo Vitamínico B/análise , Animais , Búfalos , Calibragem , Bovinos , Feminino , Cabras , Isomerismo
2.
Talanta ; 206: 120190, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514825

RESUMO

Raman spectroelectrochemistry based on electrochemical surface-enhanced Raman scattering (EC-SERS) effect is an interesting alternative to overcome the lack of sensitivity of normal Raman spectroscopy. Electrochemical activation of metallic screen-printed electrodes (SPEs) leads to the reproducible generation of nanostructures with excellent SERS properties. In that way, gold SPEs circumvent the traditional reproducibility limitation and produce the enhancement of the Raman intensity to favor the detection of low concentrations. Furthermore, fingerprint features of Raman spectroscopy make possible the dynamic spectroelectrochemical analysis of B vitamins. The accuracy assignments of Raman bands associated with B1, B2, B3, B6 and B12 vitamins present in multivitamin complexes provides valuable information, allowing us not only the detection of B vitamin present in mixtures, but also to understand the interaction between vitamins and metallic SERS surfaces.


Assuntos
Niacinamida/análise , Riboflavina/análise , Tiamina/análise , Vitamina B 12/análise , Vitamina B 6/análise , Complexo Vitamínico B/análise , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Eletrodos , Ouro/química , Nanopartículas Metálicas/química , Reprodutibilidade dos Testes , Análise Espectral Raman/métodos
3.
Biosens Bioelectron ; 146: 111733, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31600624

RESUMO

Plasmon-enhanced fluorescence (PEF) is an emerging technology for sensitive detection. It relies on the plasmonic effect of a noble metal nanostructure to dramatically enhance the fluorescence of target fluorophores around the metal surface. Because there is a compromise between plasmonic enhancement and fluorescence quenching, it is critical to control the distance between the fluorophore and the metal surface to an appropriate range. This makes the fabrication of plasmonic nanostructures for PEF assays a challenging task. Herein, we report a controllably prepared core-shell plasmonic nanostructure coated with molecularly imprinted polymer (MIP) for sensitive and specific PEF assay. Riboflavin (RF) was used as a test compound in this study. RF-imprinted Ag@SiO2 nanoparticles were prepared in a controllable manner, providing an optimal distance between the metal surface and RF molecules. The obtained hybrid nanostructure allowed for sensitive detection and specific recognition towards the target. Based on the plasmonic hybrid nanostructure, a sensitive and specific PEF assay of RF was developed and successfully applied to the determination of RF in human urine. Thus, the study paved the way for controllable preparation of molecularly imprinted plasmonic nanostructures for sensitive and specific PEF assays.


Assuntos
Nanopartículas/química , Riboflavina/urina , Dióxido de Silício/química , Prata/química , Complexo Vitamínico B/urina , Técnicas Biossensoriais/métodos , Fluorescência , Corantes Fluorescentes/química , Humanos , Impressão Molecular/métodos , Polímeros/química , Riboflavina/análise , Espectrometria de Fluorescência/métodos , Complexo Vitamínico B/análise
4.
Se Pu ; 37(11): 1157-1161, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642267

RESUMO

A method using ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of nine B vitamins in peptone. The samples were extracted with water. The analytes were separated on a Syncronis C18 column (100 mm×2.1 mm, 1.7 µm). The nine B vitamins were detected by ESI-MS/MS under the multiple reaction monitoring (MRM) mode, and the analysis was completed in 8 min. Quantification analysis was performed by using the external standard method. The correlation coefficients (R2) of the nine B vitamins in their linear ranges were greater than 0.999. The limits of detection were 0.09-1.67 µg/L. The relative standard deviations of the method were less than 3% (n=6). The mean recoveries of the nine B vitamins were 80.2%-103.9% at different spiked levels. The method is simple, accurate and sensitive, and is suitable for the determination of the nine B vitamins in peptone.


Assuntos
Peptonas/química , Complexo Vitamínico B/análise , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem
5.
Food Chem ; 298: 125010, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31284091

RESUMO

Vitamin B12 dietary supplement can be critical to the alleviation strategies against micronutrient malnutrition and food insecurity. An HPLC-DAD method has been developed and validated, per AOAC SMPR 2016.017 (Standard Method Performance Requirements), for the quantitation of four bioactive forms of vitamin B12 (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, methylcobalamin) from dietary ingredients and supplements. The method achieves chromatographic baseline resolution of vitamin B12 forms on a modern column platform without the expensive requirement of an ultra-high pressure liquid chromatography and/or mass spectrometry. The method has a wide analytical range (0.0005%w/w-85%w/w), high precision (reproducibility relative standard deviations ranged from 1.43% to 4.67%), and high accuracy (>96% spike recovery rate for 11 out of 12 accuracy testing data points). The method detection and quantification limits are less than 0.16 and 0.52 µg/mL, respectively. To our best knowledge, it is simpler, less time-consuming, and more economical than other published methods for its intended uses.


Assuntos
Cromatografia de Fase Reversa/métodos , Suplementos Nutricionais/análise , Vitamina B 12/análise , Cobamidas/análise , Laboratórios , Limite de Detecção , Vitamina B 12/análogos & derivados , Complexo Vitamínico B/análise , Complexo Vitamínico B/química
6.
Se Pu ; 37(6): 661-665, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31152518

RESUMO

A high performance quantitative capillary electrophoresis method was developed for the simultaneous determination of vitamins B1, B2, B6, nicotinamide, and calcium pantothenate in vitamin B tablets using a quantitative capillary electrophoresis instrument. The samples were extracted ultrasonically with acetonitrile-water (20:80, v/v). The automatic high precision quantitative capillary electrophoresis instrument was used to realize quantitative injection through a 10 nL injection valve. The background electrolyte was selected as 40 mmol/L sodium borate buffer (pH 9.0), which was continuously supplied by a microfluidic injection pump. The working voltage was -10 kV. The detection wavelength of vitamins B1, B2, B6, and nicotinamide was selected as 280 nm, which was then changed to 210 nm to detect calcium pantothenate. The result showed good linearities between the peak area and the concentration of vitamins B1, B2, B6, nicotinamide, and calcium pantothenate in the correlation coefficients (r) range of 0.9968-0.9998. The limits of detection (LODs) were in the range of 2.5-36.0 mg/L. The average recoveries were 94.1%-98.9% with relative standard deviations (RSDs) as 1.3%-1.9%. The method is precise, reliable, and suitable for the simultaneous determination of vitamins B1, B2, B6, nicotinamide, and calcium pantothenate in a real compound vitamin B tablet.


Assuntos
Complexo Vitamínico B/análise , Eletroforese Capilar , Niacinamida/análise , Ácido Pantotênico/análise , Riboflavina/análise , Comprimidos , Tiamina/análise , Vitamina B 6/análise
7.
Biosens Bioelectron ; 140: 111330, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31150981

RESUMO

Herein, we propose a facile chemical reduction method to synthesize the molybdenum carbide (Mo2C) nanoparticles and its application for the electrochemical detection of folic acid (FA) through imprinting technique. Raman scattering, photoelectron spectroscopy and electron microscopy techniques were employed to study the properties of Mo2C nanoparticles. FA imprinting was carried out in the presence of pyrrole monomer over Mo2C modified glassy carbon electrode (GCE). The proposed sensor showed the detection behavior for wide range of FA concentrations from 0.01 µM to 120 µM with an excellent LOD value of 4 nM and good selectivity toward FA as compared to other co-existing species in real samples. The fabricated MIP-Mo2C/GCE sensors were able to be replicated with ∼1.9% RSD, and their reproduced sensor offered good repeatability (RSD; 1.6%) and stability.


Assuntos
Ácido Fólico/sangue , Molibdênio/química , Nanopartículas/química , Complexo Vitamínico B/sangue , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Eletrodos , Ácido Fólico/análise , Humanos , Limite de Detecção , Impressão Molecular , Polimerização , Pirróis/química , Complexo Vitamínico B/análise
8.
Artigo em Inglês | MEDLINE | ID: mdl-31202182

RESUMO

The multifactorial nature of Late Onset Alzheimer's Disease (LOAD), the AD form of major relevance on epidemiological and social aspects, has driven the original investigation by LC-MS and top-down proteomics approach of the protein repertoire of the brain tissue of TgCRND8 model mice fed with a diet deficient in B vitamins. The analysis of the acid-soluble fraction of brain tissue homogenates identified a list of proteins and peptides, proteoforms and PTMs. In order to disclose possible modulations, their relative quantification in wild type and AD model mice under both B vitamin deficient and control diets was performed. The levels of metallothionein III, guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 and brain acid soluble protein 1 showed statistically significant alterations depending on genotype, diet or both effects, respectively. Particularly, metallothionein III exhibited increased levels in TgCRND8 mice under B vitamin deficient diet with respect to wild type mice under both diets. Brain acid soluble protein 1 showed the opposite, revealing decreased levels in all diet groups of AD model mice with respect to wild type mice in control diet. Lower levels of brain acid soluble protein 1 were also observed in wild type mice under deficiency of B vitamins. These results, besides contributing to increase the knowledge of AD at molecular level, give new suggestions for deeply investigating metallothionein III and brain acid soluble protein 1 in AD.


Assuntos
Doença de Alzheimer/metabolismo , Encéfalo/metabolismo , Hiper-Homocisteinemia/metabolismo , Proteoma/metabolismo , Complexo Vitamínico B/análise , Doença de Alzheimer/genética , Peptídeos beta-Amiloides/metabolismo , Animais , Química Encefálica , Proteínas de Ligação a Calmodulina/genética , Proteínas de Ligação a Calmodulina/metabolismo , Cromatografia Líquida , Proteínas do Citoesqueleto/genética , Proteínas do Citoesqueleto/metabolismo , Modelos Animais de Doenças , Feminino , Humanos , Hiper-Homocisteinemia/etiologia , Hiper-Homocisteinemia/genética , Masculino , Espectrometria de Massas , Camundongos , Camundongos Transgênicos , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Proteoma/química , Proteoma/genética , Complexo Vitamínico B/metabolismo
9.
Nutrients ; 11(6)2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31216671

RESUMO

Folate, vitamin B2, vitamin B6, vitamin B12, choline, and betaine are nutrients involved in the 1-carbon cycle that can alter the levels of DNA methylation and influence genesis and/or tumor progression. Thus, the objective of this study was to evaluate the association of folate and vitamins involved in the 1-carbon cycle and MTHFR polymorphisms in global DNA methylation in patients with colorectal cancer gene. The study included 189 patients with colorectal adenocarcinoma answering a clinical evaluation questionnaire and the Food Frequency Questionnaire (FFQ) validated for patients with colon and rectal cancer. Blood samples were collected for evaluation of MTHFR gene polymorphisms in global DNA methylation in blood and in tumor. The values for serum folate were positively correlated with the equivalent total dietary folate (total DFE) (rho = 0.51, p = 0.03) and global DNA methylation (rho = 0.20, p = 0.03). Individuals aged over 61 years (p = 0.01) in clinicopathological staging III and IV (p = 0.01) and with + heterozygous mutated homozygous genotypes for the MTHFR A1298C gene had higher levels of global DNA methylation (p = 0.04). The association between dietary intake of folate, serum folate, and tumor stage were predictive of global DNA methylation in patients' blood. The levels of serum folate, the dietary folate and the status of DNA methylation can influence clinicopathological staging.


Assuntos
Adenocarcinoma/genética , Neoplasias Colorretais/genética , Metilação de DNA/efeitos dos fármacos , Dieta/efeitos adversos , Ácido Fólico/análise , Metilenotetra-Hidrofolato Redutase (NADPH2)/sangue , Complexo Vitamínico B/análise , Adenocarcinoma/sangue , Adenocarcinoma/patologia , Ciclo do Carbono/efeitos dos fármacos , Neoplasias Colorretais/sangue , Neoplasias Colorretais/patologia , Inquéritos sobre Dietas , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Polimorfismo de Nucleotídeo Único
10.
Food Chem ; 280: 59-64, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30642507

RESUMO

The effects of washing, soaking, and cooking (ordinary cooking, high-pressure cooking, and microwave cooking) on thiamine, riboflavin, phytic acid (PA), and mineral contents (Mg, Ca, Mn, Zn, and Fe) of different cultivars rice, Xinfeng 2 and T-You 15, were investigated. Washing caused a significant loss in vitamin B, PA, and mineral contents, whereas, soaking only decreased the thiamine content. Moreover, cooking decreased the vitamin B and PA contents, and high-pressure cooking exerted more remarkable effects than those of ordinary and microwave cooking. Furthermore, washing and soaking increased the Zn and Ca bioaccessibilities, however, cooking improved the bioaccessibilities of Mg, Fe, and Ca, but decrease those of Zn and Mn. Therefore, vitamin and mineral losses caused by excessive washing should be given attention. Cooking processes in relatively low temperature may also be beneficial to the nutritive properties of vitamins and minerals.


Assuntos
Culinária/métodos , Espectrometria de Massas , Minerais/análise , Oryza/química , Ácido Fítico/análise , Complexo Vitamínico B/análise , Cálcio/análise , Micro-Ondas , Minerais/química , Oryza/metabolismo , Zinco/análise
11.
J AOAC Int ; 102(2): 445-450, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30227900

RESUMO

Background: The determination of cyanocobalamin (vitamin B12) and biotin has always been challenging because of the lack of a chromophore for biotin and trace level input for vitamin B12 in supplements. Microbiological assay methods are currently used for quantitation. However, these methods are time consuming, may lack specificity, and have high imprecision. Objective: Our laboratory developed and validated an LC method for the simultaneous quantitation of vitamin B12 and biotin. Methods: This LC method uses a single quadruple mass analyzer to detect biotin and vitamin B12 at m/z 245.10 and m/z 678.29, respectively. Results: The mass analyzer allows for low limits of quantitation (biotin: 1 ng/mL; vitamin B12: 4 ng/mL). Precision results showed that injections are repeatable without the use of an internal standard (RSD < 5%). Single analyst (n = 5: RSD < 3%), within lab (n = 10: RSD < 8%), and multilab (n = 20: RSD < 13%) precision results were also much better than those reported by microbiological assay methods. Linearity was evaluated between 92.00 ng/mL and 9200 ng/mL (R² 09916) for biotin and between 4.846 ng/mL and 484.6 ng/mL (R² 0.9999) for vitamin B12. The method is accurate between 20 ng/mL and 60 ng/mL for vitamin B12 and between 400 ng/mL and 1200 ng/mL for biotin. Conclusions: The results show a simple, accurate, and precise method for the quantitation of vitamin B12 and biotin. Highlights: This work demonstrates that single quadrupole mass analyzers can be successfully used to quantify trace level analytes in quality control laboratories.


Assuntos
Biotina/administração & dosagem , Biotina/análise , Suplementos Nutricionais , Complexo Vitamínico B/administração & dosagem , Complexo Vitamínico B/análise , Cromatografia Líquida , Humanos , Modelos Lineares , Conformação Molecular , Comprimidos/administração & dosagem , Comprimidos/análise
12.
Bioelectrochemistry ; 126: 38-47, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30472570

RESUMO

Here, an ultrasensitive and low-cost electrochemical aptasensing assay is developed based on the applicability of a fabricated nanocomposite from nitrogen-doped graphene quantum dots (N-GQDs) and gold nanoparticles (AuNPs). A modified glassy carbon electrode (GCE) with the AuNPs@N-GQDs nanocomposite (AuNPs@N-GQDs/GCE) as an efficient platform has some unique properties such as high surface area and electrical conductivity. Furthermore, the prepared platform is capable of more loading of aptamer (Apt) molecules as a biological recognition element of Ibuprofen (IBP) on the modified electrode surface. It is noteworthy that in this study, riboflavin (RF) as a universal green probe is used for the first time for electrochemical detection of IBP. According to the proposed strategy and under the optimum condition, the unprecedented detection limit (LOD) of this assay (33.33 aM) is lower than previously reported analytical methods. The results demonstrate the ability of the nanocomposite for designing of the aptasensor, integrated within the electrode format, to cheaper and simpler detection of the IBP with a specificity and sensitivity sufficient for analysis in real samples. It seems that the proposed strategy based on the AuNPs@N-GQDs nanocomposite can be expanded to other nanomaterials. So, this is expected to have promising implications in the design of electrochemical sensors or biosensors for the detection of various targets.


Assuntos
Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Ouro/química , Grafite/química , Ibuprofeno/análise , Nanocompostos/química , Riboflavina/análise , Poluentes Químicos da Água/análise , Anti-Inflamatórios não Esteroides/análise , Anti-Inflamatórios não Esteroides/sangue , Técnicas Biossensoriais/instrumentação , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Humanos , Ibuprofeno/sangue , Limite de Detecção , Nanocompostos/ultraestrutura , Nitrogênio/química , Pontos Quânticos/química , Pontos Quânticos/ultraestrutura , Riboflavina/sangue , Complexo Vitamínico B/análise , Complexo Vitamínico B/sangue , Águas Residuárias/análise , Poluentes Químicos da Água/sangue
13.
J Pharm Sci ; 107(11): 2837-2846, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30005987

RESUMO

It has been demonstrated that lyophilized drug formulations have an increased propensity to leach substances from the rubber stoppers comprising their primary packaging system when compared to aqueous liquid formulations stored in the same manner. Unfortunately, patient exposure to leachables originating in lyophilized drug products is not known. To that end, the goal of this study was to assess patient exposure to these leachables after reconstitution, storage, and administration of the lyophilized drug. To achieve this goal, several leachables present in 2 commercial lyophilized drug products were quantified after contact with polyvinyl chloride and non-polyvinyl chloride medication bags as well as an infusion set for durations of 15 min to 7 days at refrigerated and ambient temperature. The results obtained from this study showed that the bag's material of construction and the drugs formulation did not impact the mass of the leachables administered. Conversely, the mass of each leachable administered to the patient was reduced or eliminated as the contact duration with the intravenous bag and the temperature increased. However, for shorter contact durations, refrigerated storage, and higher molecular weight compounds, the patient would be exposed to a majority of the leachables originating from the vial.


Assuntos
Contaminação de Medicamentos , Embalagem de Medicamentos , Liofilização , Leucovorina/administração & dosagem , Polímeros/análise , Complexo Vitamínico B/administração & dosagem , Composição de Medicamentos , Sistemas de Liberação de Medicamentos , Armazenamento de Medicamentos , Humanos , Injeções , Leucovorina/análise , Espectrometria de Massas , Plastificantes/administração & dosagem , Plastificantes/análise , Polímeros/administração & dosagem , Borracha/administração & dosagem , Borracha/análise , Temperatura , Complexo Vitamínico B/análise
14.
J Food Sci ; 83(8): 2183-2190, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30059142

RESUMO

Multiyear spaceflight will require innovative strategies to preserve vitamins in foods, in order to retain astronaut health and functionality long-term. Furthermore, space foods must be low weight/volume to comply with cargo restrictions, and must retain sensory quality to ensure consumption. Low water activity products were developed, fortified with vitamins A, B1, B9, C, and E at twice their spaceflight requirements, stored for three years at 21 °C and one year at 38 °C, and analyzed annually for retained vitamin content and organoleptic quality. The vitamins were encapsulated in carbohydrate or lipid coatings and tested in relatively low and high fat versions of compressed bars and powdered drink mix formulations. Susceptibility to degradation at 21 °C followed the sequence B9 > A > B1 > C > E; degradation rates were fitted to first-order kinetics. Vitamins A and C were more sensitive at the higher storage temperature. Vitamin retention was slightly higher in compressed bars verses powders. Effects of matrix lipid level on stability were vitamin dependent. Sensory characteristics for products stored three years at 21 °C remained mostly above 6.0 on a 9-point hedonic scale, whereas those stored for one year at 38 °C remained mostly above 5.0. Compressed bars firmed significantly during storage. All vitamin levels after three years at 21 °C remained above the space flight requirements, demonstrating the suitability of the selected fortification schemes and food matrices for long-term preservation. PRACTICAL APPLICATION: Vitamins A, B1, B9, C, and E were retained at 70% to 95% levels for three years at 21 °C in carefully formulated, fortified low water activity products. Product compression and vacuum packaging slightly helped to further preserve vitamins. The sensory quality of these products was retained throughout storage.


Assuntos
Alimentos em Conserva/análise , Sensação , Voo Espacial , Vitaminas/química , Ácido Ascórbico/análise , Estabilidade de Medicamentos , Embalagem de Alimentos/métodos , Alimentos Fortificados/análise , Humanos , Estados Unidos , United States National Aeronautics and Space Administration , Vitamina A/análise , Complexo Vitamínico B/análise , Vitamina E/análise , Vitaminas/análise
15.
Food Chem ; 264: 386-392, 2018 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-29853391

RESUMO

Non-traditional wild rice flakes were analysed for chemical composition, vitamin B compounds, α-tocopherol, mineral and trace elements. Dietary intakes of vitamins, minerals and trace elements were evaluated using FAO/WHO and Institute of Medicine regulations. Wild rice flakes proved to be significant contributors of pyridoxine, pantothenic and folic acids, niacin, thiamine, chromium, magnesium, manganese, phosphorus, zinc, copper, molybdenum and iron to essential dietary intakes values. Toxic dietary intake values for aluminium, cadmium, tin and mercury were less than 33%, which complies the limits for adults set by FAO/WHO for toxic elements intake related to the body weight of 65 kg for females and 80 kg for males taking 100 g of flakes as a portion. However, concentrations of Hg reaching between 3.67 and 12.20 µg/100 g in flakes exceeded the average Hg value of 0.27-1.90 µg/100 g in cereals consumed in the EU. It has to be respected in the future.


Assuntos
Minerais/análise , Oryza/química , Oligoelementos/análise , Complexo Vitamínico B/análise , Adulto , Cromatografia Líquida de Alta Pressão , Dieta , Feminino , Humanos , Masculino , Espectrometria de Massas , Metais Pesados/análise , Política Nutricional , Oryza/metabolismo , alfa-Tocoferol/análise
16.
Adv Nutr ; 9(suppl_1): 358S-366S, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29846529

RESUMO

Despite the critical role of vitamin B-12 in infant development, existing recommendations for infant and maternal intake during lactation are based on milk vitamin B-12 concentrations analyzed with outdated methods in a sample of 9 Brazilian women. Accurate quantification of vitamin B-12 in the milk matrix requires effective hydrolysis of the vitamin from haptocorrin, its binding protein. The objective of the present systematic review is to consider and critique evidence of associations between milk vitamin B-12 concentration and time postpartum, maternal vitamin B-12 consumption, maternal vitamin B-12 status, and sample collection methodology. A systematic search of published literature was undertaken using the US National Library of Medicine's MEDLINE/PubMed bibliographic search engine. Observational and intervention studies were included if research was original and vitamin B-12 concentration in human milk was measured using an appropriate method during the first 12 mo of lactation. Eleven studies met inclusion criteria. Vitamin B-12 concentration was highest in colostrum and decreased in a poorly delineated trajectory over the first 3-4 mo of lactation. There was some evidence of a positive association between habitual maternal vitamin B-12 intake and milk vitamin B-12 concentration in marginally nourished women. Supplementation with 50-250 µg vitamin B-12/d during pregnancy and lactation raised human milk vitamin B-12 concentrations while intervention was ongoing, whereas supplementation with 2.6-8.6 µg/d was effective in a population with poor baseline vitamin B-12 status but not in other populations. Whether milk vitamin B-12 concentration varies with maternal circulating vitamin B-12 concentrations or sampling methodology requires further research as existing data are conflicting. Additional research is needed to bridge knowledge gaps in the understanding of human milk vitamin B-12 concentrations. Reference values for vitamin B-12 in human milk and recommended intakes during infancy and lactation should be reevaluated using modern methods of analysis.


Assuntos
Aleitamento Materno , Dieta , Lactação , Leite Humano/química , Estado Nutricional , Vitamina B 12/análise , Complexo Vitamínico B/análise , Suplementos Nutricionais , Feminino , Humanos , Lactente , Fenômenos Fisiológicos da Nutrição do Lactente , Fenômenos Fisiológicos da Nutrição Materna , Necessidades Nutricionais , Gravidez , Vitamina B 12/administração & dosagem , Vitamina B 12/uso terapêutico , Deficiência de Vitamina B 12/tratamento farmacológico , Deficiência de Vitamina B 12/etiologia , Complexo Vitamínico B/administração & dosagem , Complexo Vitamínico B/uso terapêutico
17.
Nutrition ; 50: 8-17, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29518603

RESUMO

OBJECTIVES: To determine dietary patterns (DPs) and explain the highest variance of vitamin B6, folate, and B12 intake and related concentrations among European adolescents. METHODS: A total of 2173 adolescents who participated in the Healthy Lifestyle in Europe by Nutrition in Adolescence study met the eligibility criteria for the vitamin B intake analysis (46% boys) and 586 adolescents for the biomarkers analysis (47% boys). Two non-consecutive, 24-h, dietary recalls were used to assess the mean intakes. Concentrations were measured by chromatography and immunoassay testing. A reduced rank regression was applied to elucidate the combined effect of food intake of vitamin B and related concentrations. RESULTS: The identified DPs (one per vitamin B intake and biomarker and by sex) explained a variability between 34.2% and 23.7% of the vitamin B intake and between 17.2% and 7% of the biomarkers. In the reduced rank regression models, fish, eggs, cheese, whole milk and buttermilk intakes were loaded positively for vitamin B intake in both sexes; however, soft drinks and chocolate were loaded negatively. For the biomarkers, a higher variability was observed in the patterns in terms of food loads such as alcoholic drinks, sugars, and soft drinks. Some food items were loaded differently between intakes and biomarkers such as fish products, which was loaded positively for intakes but negatively for plasma folate in girls. CONCLUSIONS: The identified DPs explained up to 34.2% and 17.2% of the variability of the vitamin B intake and plasma concentrations, respectively, in European adolescents. Further studies are needed to elucidate the factors that determine such patterns.


Assuntos
Dieta/estatística & dados numéricos , Ácido Fólico/análise , Vitamina B 12/análise , Vitamina B 6/análise , Complexo Vitamínico B/análise , Adolescente , Biomarcadores/sangue , Estudos Transversais , Inquéritos sobre Dietas , Ingestão de Alimentos , Europa (Continente) , Feminino , Estilo de Vida Saudável , Humanos , Masculino , Avaliação Nutricional , Estado Nutricional , Análise de Regressão
18.
Fish Shellfish Immunol ; 76: 333-346, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29544771

RESUMO

In this study, we investigated the effects of dietary myo-inositol on the intestinal immune barrier function and related signaling pathway in young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (221.33 ±â€¯0.84 g) were fed six diets containing graded levels of myo-inositol (27.0, 137.9, 286.8, 438.6, 587.7 and 737.3 mg/kg) for 10 weeks. After the growth trial, fish were challenged with Aeromonas hydrophila. The results indicated that compared with the optimal dietary myo-inositol level, myo-inositol deficiency (27.0 mg/kg diet): (1) decreased lysozyme (LZ) and acid phosphatase (ACP) activities, as well as complement 3 (C3), C4 and immunoglobulin M (IgM) contents in the proximal intestine (PI), middle intestine (MI) and distal intestine (DI) of young grass carp (P < 0.05). (2) down-regulated the mRNA levels of anti-microbial substance: liver expressed antimicrobial peptide (LEAP) 2A, LEAP-2B, hepcidin, ß-defensin-1 and mucin2 in the PI, MI and DI of young grass carp (P < 0.05). (3) up-regulated pro-inflammatory cytokines [IL-1ß (not in DI), TNF-α and IL-8], nuclear factor kappa B P65 (not NF-κB P52), c-Rel, IκB kinaseα (IKKα), IKKß and IKKγ mRNA levels in the PI, MI and DI of young grass carp (P < 0.05); and down-regulated pro-inflammatory cytokines IL-15 (not in DI) and inhibitor of κBα (IκBα) mRNA levels (P < 0.05). (4) down-regulated the mRNA levels of anti-inflammatory cytokines [IL-10 (not in DI), IL-11, IL-4/13B (not IL-4/13A), TGF-ß1 and TGF-ß2], target of rapamycin (TOR), eIF4E-binding proteins 1 (4E-BP1) and ribosomal protein S6 kinase 1 (S6k1) in the PI, MI and DI of young grass carp (P < 0.05). All data indicated that myo-inositol deficiency could decrease fish intestine immunity and cause inflammation under infection of A. hydrophila. Finally, the optimal dietary myo-inositol levels for the ACP and LZ activities in the DI were estimated to be 415.1 and 296.9 mg/kg diet, respectively.


Assuntos
Carpas/genética , Carpas/imunologia , Inositol/deficiência , Intestinos/imunologia , Transdução de Sinais/imunologia , Complexo Vitamínico B/análise , Ração Animal/análise , Animais , Dieta/veterinária , Suplementos Nutricionais/análise , NF-kappa B/metabolismo , Serina-Treonina Quinases TOR/metabolismo
19.
Talanta ; 181: 13-18, 2018 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-29426491

RESUMO

This paper describes the development of a novel electrochemical assay for the measurement of water-soluble vitamins in food and pharmaceutical products. The optimum conditions for the determination of vitamin B1 (thiamine), B2 (riboflavin) and B6 (pyridoxine) in phosphate buffer were established using cyclic voltammetry in conjunction with screen printed carbon electrodes (SPCEs). The optimum current response for all three vitamins was achieved in 0.1M phosphate buffer pH 11 using an initial potential of -1.0V. Using square wave voltammetry, the linear ranges for thiamine, riboflavin, and pyridoxine were found to be: 15-110µg/ml, 0.1-20µg/ml, and 2-80µg/ml respectively. The application of the method to a commercial food product yielded a recovery of 95.78% for riboflavin, with a coefficient of variation (CV) of 3.38% (n = 5). The method was also applied to a multi-vitamin supplement for the simultaneous determination of thiamine, riboflavin and pyridoxine. In both cases only simple dilution with buffer followed by centrifugation was required prior to analysis. The resulting square wave voltammetric signals were completely resolved with Ep values of -0.7V, +0.2V, and +0.6V respectively. The recoveries determined for the vitamin B complex in a commercial supplement product were found to be 110%, 114%, and 112% respectively (CV = 7.14%, 6.28%. 5.66% respectively, n = 5).


Assuntos
Técnicas Biossensoriais/métodos , Carbono/química , Suplementos Nutricionais/análise , Técnicas Eletroquímicas/métodos , Análise de Alimentos/métodos , Complexo Vitamínico B/análise , Reprodutibilidade dos Testes , Riboflavina/análise , Tiamina/análise , Vitamina B 6/análise
20.
Talanta ; 180: 108-119, 2018 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-29332787

RESUMO

The aim of the present work was to develop a rapid and interference-free method based on liquid chromatography-mass spectrometry (LC-MS) for the simultaneous determination of nine B-group vitamins in various energy drinks. A smart and green strategy that modeled the three-way data array of LC-MS with second-order calibration methods based on alternating trilinear decomposition (ATLD) and alternating penalty trilinear decomposition (APTLD) algorithms was developed. By virtue of "mathematical separation" and "second-order advantage", the proposed strategy successfully solved the co-eluted peaks and unknown interferents in LC-MS analysis with the elution time less than 4.5min and simple sample preparation. Satisfactory quantitative results were obtained by the ATLD-LC-MS and APTLD-LC-MS methods for the spiked recovery assays, with the average spiked recoveries ranging from 87.2-113.9% to 92.0-111.7%, respectively. These results acquired from the proposed methods were confirmed by the LC-MS/MS method, which shows a quite good consistency with each other. All these results demonstrated that the developed chemometrics-assisted LC-MS strategy had advantages of being rapid, green, accurate and low-cost, and it could be an attractive alternative for the determination of multiple vitamins in complex food matrices, which required no laborious sample preparation, tedious condition optimization or more sophisticated instrumentations.


Assuntos
Cromatografia Líquida/métodos , Bebidas Energéticas/análise , Análise de Alimentos/métodos , Espectrometria de Massas em Tandem/métodos , Complexo Vitamínico B/análise , Algoritmos , Calibragem , Cromatografia Líquida/economia , Análise de Alimentos/economia , Limite de Detecção , Modelos Lineares , Niacinamida/análise , Ácido Pantotênico/análise , Espectrometria de Massas por Ionização por Electrospray/economia , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem/economia , Tiamina/análise , Vitamina B 12/análise , Vitamina B 6/análise
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