Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.508
Filtrar
1.
Food Chem ; 328: 126930, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-32485581

RESUMO

The objective of this study was to quantify the phenolic compounds and to evaluate and compare the biological activities of the ethyl acetate (EtOAc), methanolic (MeOH) and aqueous extracts from the Micromeria nervosa aerial parts, based on their antioxidant activity and enzymatic inhibition. Total phenolic and flavonoid contents were calculated and individual compo3unds were detected using LC-ESI-MS/MS. The antioxidant activity was determined using six different assays while enzymatic activity was determined by α-amylase and tyrosinase enzyme inhibition. The main phenolic constituents detected in the extracts were rosmarinic acid. In the antioxidant assays the aqueous extract was shown to be more efficient than the others. The EtOAc and MeOH extracts presented higher inhibitory activity with respect to α-amylase and tyrosinase. Regardless of the solvent, the results suggest M. nervosa aerial extracts present a biological potential due to their antioxidant activity and enzymatic inhibition.


Assuntos
Antioxidantes/química , Inibidores Enzimáticos/análise , Lamiaceae/química , Fenóis/análise , Extratos Vegetais/química , Espectrometria de Massas em Tandem , Cromatografia Líquida de Alta Pressão , Cinamatos/análise , Depsídeos/análise , Flavonoides/análise , Lamiaceae/metabolismo , Monofenol Mono-Oxigenase/antagonistas & inibidores , Monofenol Mono-Oxigenase/metabolismo , Componentes Aéreos da Planta/química , Componentes Aéreos da Planta/metabolismo , alfa-Amilases/antagonistas & inibidores , alfa-Amilases/metabolismo
2.
Z Naturforsch C J Biosci ; 75(3-4): 121-128, 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32267249

RESUMO

Plants of the Plantago genus are widely used in Turkish folk medicine especially for the treatment of wound, abscess, and inflammation. The aqueous extract and five phenylethanoid glycosides acteoside (1), arenarioside (2), echinacoside (3), isoacteoside (4), and leucosceptoside A (5) isolated from the aerial parts and roots of Plantago holosteum Scop. (Plantaginaceae) were tested for their possible inhibitory activity against hyaluronidase, elastase, and collagenase, related to wound pathogenesis. Even though the aqueous extract prepared from the aerial parts (36.26%) and roots (47.01%) and the isolated compounds acteoside (29.13%), echinacoside (28.73%), and isoacteoside (31.69%) exerted a notable inhibition, arenarioside and leucosceptoside A were found inactive in the hyaluronidase enzyme inhibition test. Similar results were obtained from the collagenase enzyme inhibition test. The aqueous extract prepared from the aerial parts (31.09%) and roots (35.17%), echinacoside (25.13%), and isoacteoside (23.85%) exerted a notable inhibition in this test. However, none of the extracts and isolated compounds displayed elastase enzyme inhibitory activity. The experimental data demonstrated that P. holosteum displayed a remarkable enzyme inhibitory activity against hyaluronidase and collagenase. This paper is the first report regarding the in vitro enzyme inhibitory activity of P. holosteum.


Assuntos
Colagenases/metabolismo , Glicosídeos/farmacologia , Hialuronoglucosaminidase/antagonistas & inibidores , Elastase Pancreática/antagonistas & inibidores , Plantago/química , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Glucosídeos/química , Glucosídeos/farmacologia , Glicosídeos/química , Humanos , Medicina Tradicional , Estrutura Molecular , Fenóis/química , Fenóis/farmacologia , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Raízes de Plantas/química , Turquia
3.
Ann Agric Environ Med ; 27(1): 29-35, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32208576

RESUMO

INTRODUCTION AND OBJECTIVE: Contamination of soil by mercury poses several risks to human health through consumption of fruits and vegetables. In Slovakia, a high concentration of mercury is found in the soil of the Central Spis region. The objective of the study is to measure the mercury concentrations in the parts of selected plant species and trees growing within 100 meters of a former ore processing facility. MATERIAL AND METHODS: A total of 24 samples of plants, 20 samples of parts of needle-leaved trees and 9 samples of parts of broad-leaved trees were collected from soils with a high concentration of mercury. The concentration was measured by atomic absorption spectrometry in different parts of the plants: leaves - 18 species, roots - 15 species, stems - 11 species, flowers - 7 species), and different parts of trees (crust - 8 species, branches - 8 species, needles - 5 species, cones - 5 species, leaves - 3 species). RESULTS: The concentrations of mercury in the soils taken at a depth of 0.25 m exceeded the maximum allowed levels more than 50-times. Potatoes, parsley and carrots from these soils exceeded the maximum allowed mercury levels 6-times, 5-times and twice, respectively. The average concentrations of mercury in the roots of 2-year onions exceeded the limit more than 50-times. The flowers of cornflower contain 18.20 mg*kg -1 , leaves of dandelion 10.61 mg*kg -1 and roots of plantain 6.80 mg*kg -1 of mercury. Regarding trees, the highest concentrations were found in the branches of juniper and leaves of aspen - more than 1 mg*kg -1. CONCLUSIONS: The systematic monitoring of mercury is still very important, since it was found that the end of ore processing does not solve the issue of contamination in the Central Spis region. Therefore, the consumption of fruits and vegetables from the areas of former ore processing facilities is not recommended.


Assuntos
Mercúrio/análise , Componentes Aéreos da Planta/química , Raízes de Plantas/química , Poluentes do Solo/análise , Monitoramento Ambiental , Contaminação de Alimentos/análise , Metalurgia , Eslováquia , Árvores/química , Verduras/química
4.
PLoS One ; 15(3): e0230645, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32210452

RESUMO

Houttuynia cordata Thunb. has been used as a traditional medicine to treat a variety of ailments in Asian countries such as China, Japan, South Korea, and Thailand. In Thailand, H. cordata fermentation products (HCFPs) are commercially produced and popularly consumed throughout the country without experimental validation. Anti-inflammatory activity of H. cordata fresh leaves or aerial parts has previously been reported, however, the anti-inflammatory activity of the commercially available HCFPs produced by the industrialized process has not yet been investigated. The aim of this study was to evaluate in vitro and in vivo anti-inflammatory potential of the selected industrialized HCFP. LPS-induced RAW264.7 and carrageenan-induced paw edema models were used to evaluate the anti-inflammatory activity of HCFP. The phenolic acid components of HCFP aqueous and methanolic extracts were investigated using HPLC analysis. In RAW264.7 cells, the HCFP aqueous and methanolic extracts reduced NO production and suppressed LPS-stimulated expression of PGE2, iNOS, IL-1ß, TNF-α and IL-6 levels in a concentration-dependent manner, however, less effect on COX-2 level was observed. In Wistar rats, 3.08 and 6.16 mL/kg HCFP reduced paw edema after 2 h carrageenan stimulation, suggesting the second phase anti-edematous effect similar to diclofenac (150 mg/kg). Whereas, 6.16 mL/kg HCFP also reduced paw edema after 1 h carrageenan stimulation, suggesting the first phase anti-edematous effect. Quantitative HPLC revealed the active phenolic compounds including syringic, vanillic, p-hydroxybenzoic and ferulic acids, which possess anti-inflammatory activity. Our results demonstrated for the first time the anti-inflammatory activity of the industrialized HCFP both in vitro and in vivo, thus validating its promising anti-inflammation potential.


Assuntos
Anti-Inflamatórios/farmacologia , Suplementos Nutricionais/análise , Houttuynia/metabolismo , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/uso terapêutico , Sobrevivência Celular/efeitos dos fármacos , Edema/induzido quimicamente , Edema/tratamento farmacológico , Houttuynia/química , Interleucina-1beta/genética , Interleucina-1beta/metabolismo , Lipopolissacarídeos/toxicidade , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Masculino , Camundongos , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/metabolismo , Fenóis/análise , Componentes Aéreos da Planta/química , Componentes Aéreos da Planta/metabolismo , Extratos Vegetais/química , Folhas de Planta/química , Folhas de Planta/metabolismo , Células RAW 264.7 , Ratos , Ratos Wistar
5.
BMC Complement Med Ther ; 20(1): 79, 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32164639

RESUMO

BACKGROUND: In the context of searching for potent, safe, natural antimicrobial agents to combate the global antimicrobial resistance (AMR) phenomenon, the current study evaluates for the first time ever, the broad-spectrum antimicrobial activity of essential oil (EO) and extracts from the rare wild plant Centaurea pumilio L.. It has tremendous ethnomedicinal values; its dried root is used as a fattening agent, a treatment for bad breath and diabetes, and screened for schistosomicidal activity. METHODS: C. pumilio EO was extracted by hydrodistillation using a Clevenger apparatus. Chemical constituents of aerial part were extracted using a sequential solvent/solvent procedure employing four solvents with increasing polarities in the following order: petroleum ether, chloroform, ethyl acetate, and n-butanol. The chemical constituents were identified by GC-MS. Fifty-two microbial strains were used; twenty-six multidrug resistant (MDR), sixteen clinical, and ten reference strains. The identification of the microbial strains was performed by MALDI-TOF-MS. The antimicrobial activity of the EO and the aerial part and the root extracts was assessed through disc diffusion assay. A minimum inhibitory concentration (MIC) of the EO and extracts was determined using the broth micro-dilution method. RESULTS: The growth of reference and clinical strains was inhibited by EO, methanol, chloroform, and ethyl acetate aerial part extracts and chloroform root extract. The MDR strains growth, however, was inhibited only by EO and chloroform aerial part extract. GC-MS identified for the first time eighteen constituents from aerial part EO and chloroform extract each. EO showed antimicrobial activity against the reference, clinical, and MDR strains with MIC values of 31.25-125, 31.25-125, and 62.50-250 µg/mL, respectively. Methanol aerial part extract exhibited high antimicrobial activities with MIC values of 62.50-250 µg/mL against reference and clinical strains. Chloroform root extract displayed strong antimicrobial activity against reference and clinical strains recording MIC values of 62.50-250 µg/mL and 62.50-125 µg/mL, respectively. The chloroform aerial part extract demonstrated potent antimicrobial activity against the reference, clinical, and MDR strains with 31.25, 31.25, and 15.62 µg/mL MIC values, respectively. CONCLUSIONS: Present data unravel the C. pumilio pharmacological magnitude to discover eco-friendly potent antimicrobial agents to fight AMR phenomenon.


Assuntos
Antibacterianos/farmacologia , Centaurea/química , Farmacorresistência Bacteriana Múltipla , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Antibacterianos/química , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Raízes de Plantas/química
6.
Environ Toxicol ; 35(7): 774-782, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32061153

RESUMO

This study aims to investigate the protective effects of the Bauhinia championii (BC) against ischemia/reperfusion (I/R)-induced injury in an isolated heart model. Langendorff-perfused C57BL/6JNarl mice hearts were performed with 30 minutes ischemia and 60 minutes reperfusion by left anterior descending artery ligation. Before reperfusion, boiling water extracts of BC (10 mg/L) was pretreated for 15 minutes. During reperfusion, BC significantly decreased the occurrence of ventricular arrhythmias by lead II electrocardiogram (ECG). Electrophysiological effect of BC was further determined in isolated ventricular myocytes by whole-cell patch clamp technique. The underlying mechanism may result from its Na+ channel blocking activity characterized with reduced rise slope of action potential and Na+ current density. Moreover, BC dramatically reduced I/R-caused infarct size, which was accessed by 2,3,5-triphenyltetrazolium chloride (TTC) assay. Since BC decreased I/R-induced myoglobin release and oxidation of Ca2+ -calmodulin-dependent protein kinase, inhibition of myocardial necroptosis may account for the protective effects of BC on myocytes lose. This study indicated that BC may prevent I/R induced ventricular arrhythmias and myocyte death by blocking Na+ channels and decreasing necroptosis, respectively. Since most of the available antiarrhythmic remedies have unwanted adverse actions, BC could be a novel candidate for the treatment of myocardial infarction and ventricular arrhythmia.


Assuntos
Bauhinia/química , Coração/efeitos dos fármacos , Traumatismo por Reperfusão Miocárdica/prevenção & controle , Extratos Vegetais/farmacologia , Bloqueadores dos Canais de Sódio/farmacologia , Animais , Eletrocardiografia , Técnicas In Vitro , Masculino , Potenciais da Membrana/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Infarto do Miocárdio/metabolismo , Infarto do Miocárdio/patologia , Infarto do Miocárdio/prevenção & controle , Traumatismo por Reperfusão Miocárdica/metabolismo , Traumatismo por Reperfusão Miocárdica/patologia , Miocárdio/metabolismo , Miocárdio/patologia , Miócitos Cardíacos/metabolismo , Miócitos Cardíacos/patologia , Necroptose/efeitos dos fármacos , Técnicas de Patch-Clamp , Componentes Aéreos da Planta/química , Extratos Vegetais/isolamento & purificação , Bloqueadores dos Canais de Sódio/isolamento & purificação , Canais de Sódio/metabolismo
7.
Z Naturforsch C J Biosci ; 75(3-4): 57-64, 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32074078

RESUMO

Achillea moschata Wulfen, which grows in the Alps, is extensively used by local people for its medicinal properties. Two studied samples were collected, at the flowering stage, in Val Mustair (Switzerland) and Valchiavenna (Italy), respectively. The aerial parts were defatted with petroleum ether (PET) and successively extracted with dichloromethane (DCM) and methanol (MeOH). High-performance liquid chromatography and electrospray ionization-tandem mass spectrometry analyses of the methanolic extracts evidenced that flavonoids were the predominant compounds compared to phenolic acids in both samples (89.5 vs. 33.0 µg/mg DW in A. moschata Valchiavenna and 82.5 vs. 40.0 µg/mg DW in A. moschata Val Mustair). Among flavonoid derivatives, luteolin and apigenin were the predominant aglycones, free and glycosilated. The A. moschata Valchiavenna extract was characterized by apigenin as the main compound (60.4 µg/mg DW), while A. moschata Val Mustair was characterized by its derivative apigenin 7-O-glucoside (44.7 µg/mg DW). The antioxidant activity of all the obtained extracts was tested by the DPPH (2,2-diphenyl-picryl hydrazyl) and ABTS (2,21-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) methods, which showed their increasing scavenger capacity in relation to extract polarity (PET extract < DCM extract < MeOH extract). The extracts were also investigated against three Gram-positive (Bacillus cereus, Enterococcus faecalis and Staphylococcus aureus) and three Gram-negative (Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa) bacterial species using the disc diffusion assay. DMC and PET were the most active extracts (inhibition diameter: ≥12 mm).


Assuntos
Achillea/química , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Polifenóis/farmacologia , Antibacterianos/química , Antioxidantes/química , Cromatografia Líquida de Alta Pressão , Flavonoides/química , Flavonoides/farmacologia , Itália , Testes de Sensibilidade Microbiana , Componentes Aéreos da Planta/química , Extratos Vegetais/análise , Polifenóis/química , Metabolismo Secundário , Espectrometria de Massas por Ionização por Electrospray , Suíça
8.
Curr Pharm Biotechnol ; 21(11): 1059-1069, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32065099

RESUMO

BACKGROUND: The folkloric profile of Delonix regia demonstrates that it can be used in the management of diabetes. OBJECTIVE: The present study was conducted to evaluate the safety profile of the aerial part extracts of Delonix regia and their antidiabetic potential along with improvement in oxidative stress. MATERIALS AND METHODS: Phytochemical screening, total phenolic, and flavonoid contents along with in-vitro antioxidant and alpha-amylase inhibitory activities were determined. HPLC analysis, acute toxicity, glucose tolerance, in-vivo antidiabetic effect along with the influence on biochemical, oxidative stress parameters, and comet assay of the active extract were performed and assessed. RESULTS: Total phenolic (831.6±0.002 mg/g GAE) and flavonoid (361.4±0.002 mg/g QE) contents were found to be higher in the methanolic extract. Inhibitory concentration IC50 indicated better results for the methanolic extract in DPPH (47.6µg/mL) and alpha-amylase inhibitory (14.61µg/mL) assays. HPLC analysis of the methanolic extract confirmed the presence of quercetin, gallic acid, caffeic acid, cinnamic acid, ferulic acid, and p-coumaric acid. Acute oral toxicity exhibited no mortality and morbidity during the 24h period. The methanolic extract showed better tolerance to glucose. Streptozotocin- nicotinamide (55-110 mg/kg) induced hyperglycemia declined along with improvement in hematological, biochemical parameters and oxidative stress markers (SOD, CAT, H202) in a dose-dependent manner. The maximum effect was recorded at 500mg/kg dose. Comet assay was performed for genotoxic studies and it was observed that the methanolic extract of Delonix regia showed the maximum genoprotective effect at 100µg/mL. CONCLUSION: The findings suggest that the methanolic aerial part extract of Delonix regia exhibited hypoglycemic, antioxidant, and hypolipidemic activities. The antidiabetic effect was comparable to glibenclamide suggesting its therapeutic use as a natural anti-diabetic remedy.


Assuntos
Antioxidantes/uso terapêutico , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Tipo 2/tratamento farmacológico , Fabaceae/química , Hipoglicemiantes/uso terapêutico , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Animais , Antioxidantes/administração & dosagem , Antioxidantes/toxicidade , Dano ao DNA , Relação Dose-Resposta a Droga , Hiperglicemia/tratamento farmacológico , Hipoglicemiantes/isolamento & purificação , Hipoglicemiantes/toxicidade , Medicina Tradicional , Componentes Aéreos da Planta/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/toxicidade , Ratos Wistar
9.
Chem Pharm Bull (Tokyo) ; 68(1): 96-99, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31902905

RESUMO

Chemical investigation of the aerial parts of Andrographis paniculata resulted in isolation of nine compounds, including a new ent-labdane diterpenoid, andrographic acid methyl ester (1), a new chalcone glucoside, pashanone glucoside (5), and seven known metabolites, andrograpanin (2), andrographolide (3), andropanolide (4), andrographidine A (6), andrographidine F (7), 6-epi-8-O-acetyl-harpagide (8), and curvifloruside F (9). Their chemical structures were elucidated based on comprehensive analyses of the spectroscopic data, including NMR and MS. Among the isolated compounds, andropanolide exerted cytotoxicity toward LNCaP, HepG2, KB, MCF7, and SK-Mel2 carcinoma cells, with IC50 values ranging from 31.8 to 45.9 µM. In addition, andropanolide significantly inhibited the overproduction of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, with an IC50 value of 13.4 µM.


Assuntos
Andrographis/química , Diterpenos/química , Flavonoides/química , Andrographis/metabolismo , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Humanos , Lipopolissacarídeos/toxicidade , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Espectroscopia de Ressonância Magnética , Camundongos , Conformação Molecular , Óxido Nítrico/metabolismo , Componentes Aéreos da Planta/química , Componentes Aéreos da Planta/metabolismo , Células RAW 264.7
10.
Food Chem ; 313: 126092, 2020 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31923866

RESUMO

Trigonella foenum-graecum L. (fenugreek) is used as a leafy vegetable and spice in China and North African countries. However, the biochemical components of its aerial parts were rarely explored. In this study, the bioactivities of the various extract fractions from the aerial parts of this edible plant were assessed, the ethyl acetate extract fraction exhibited strong antioxidant and anti-inflammatory effects. Through bioassay-guided isolation, one new pterocarpan (1), as well as twelve known pterocarpans (2-13) were obtained, nine of them (5-13) were first reported in the fenugreek, four pterocarpans (9, 11-13) had strong antioxidant activity, eleven pterocarpans (1-3, 5-12) possessed obvious anti-inflammatory activity. This study indicates that pterocarpans are main bioactive components of this edible plant. Apart from its nutritional value as food, the aerial parts of this plant can also be further explored as functional foods or antioxidants in food industry.


Assuntos
Pterocarpanos/química , Trigonella/química , Animais , Anti-Inflamatórios/química , Antioxidantes/química , Conformação Molecular , Componentes Aéreos da Planta/química , Componentes Aéreos da Planta/metabolismo , Pterocarpanos/metabolismo
11.
Phytochemistry ; 171: 112230, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31923722

RESUMO

Bioactivity guided the isolation of extracts from the aerial parts Scutellaria barbata D. Don to discover neo-clerodane diterpenoids with potent phytotoxic activity. Of the 34 isolates, 13 neo-clerodane diterpenoids were described for the first time. The structures of these undescribed compounds were elucidated by extensive analysis of NMR spectroscopic data, and the absolute configurations of scutebarbolides A and L and scutebata W were determined by X-ray diffraction. The phytotoxic activity of all compounds against the growth of the roots and shoots of L. perenne and L. sativa seedlings were first reported, and some compounds showed considerable inhibitory effects, especially scutebarbolide K, whose inhibition rates were higher than those of the positive control at concentrations ranging from 25 to 200 µg/mL. When L. perenne and L. sativa seedlings were treated at a concentration of 200 µg/mL, scutebarbolide K caused wilting symptoms on and finally death of these two tested plant seedlings. In addition, the structure-activity relationships of these neo-clerodane diterpenoids were also discussed.


Assuntos
Diterpenos Clerodânicos/farmacologia , Lolium/efeitos dos fármacos , Oryza/efeitos dos fármacos , Compostos Fitoquímicos/farmacologia , Componentes Aéreos da Planta/química , Scutellaria/química , Diterpenos Clerodânicos/química , Diterpenos Clerodânicos/isolamento & purificação , Lolium/crescimento & desenvolvimento , Oryza/crescimento & desenvolvimento , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento
12.
PLoS One ; 15(1): e0227235, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31910241

RESUMO

Schizonepetae Spica (SS), the dried spike of Schizonepeta tenuifolia Briq., is a traditional Chinese medicinal herb. According to the color of persistent calyx, SS is categorized into two classes: the yellowish-green-type and the brownish-type. Based on the chemometrics analysis of gas chromatography-mass spectrometry (GC-MS), a novel model of identifying and evaluating the quality of SS in different colors was constructed for the first time in this work. 20 batches SS samples of different colors were collected and used to extract essential oils. The average essential oils yield of SS in yellowish-green color was significantly higher than that of SS in brownish color from the same origin (p<0.05). The GC-MS fingerprints of 20 batches SS samples whose correlation coefficients were over 0.964 demonstrated SS samples were consistent to some extent in spite of slightly different chemical indexes. A total of 39 common volatiles compounds were identified. Hierarchical clustering analysis (HCA), principal component analysis (PCA) and partial least-squares discriminate analysis (PLS-DA) were developed to distinguish SS samples characterized by different colors. Consistent results were obtained to show that SS samples could be successfully grouped according to their color. Finally, 4,5,6,7-tetrahydro-3,6-dimethyl-benzofuran and pulegone were detected as the key variables for discriminating SS samples of different colors and for quality control. The obtained results proved that SS of good quality were often yellowish-green and those of poor quality were often brownish.


Assuntos
Medicamentos de Ervas Chinesas/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Lamiaceae/química , Controle de Qualidade , Cor , Análise Discriminante , Medicamentos de Ervas Chinesas/normas , Óleos Voláteis/análise , Óleos Voláteis/isolamento & purificação , Componentes Aéreos da Planta/química , Análise de Componente Principal
13.
Chem Biodivers ; 17(2): e1900544, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31840372

RESUMO

Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with ß-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8-61.8 %), diterpenes (12.9-43.0 %) and fatty acids and their derivatives (6.6-21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.


Assuntos
Geranium/química , Compostos Orgânicos Voláteis/química , Análise por Conglomerados , Cromatografia Gasosa-Espectrometria de Massas , Geranium/metabolismo , Componentes Aéreos da Planta/química , Componentes Aéreos da Planta/metabolismo , Sérvia , Sesquiterpenos/química , Sesquiterpenos/isolamento & purificação , Compostos Orgânicos Voláteis/análise
14.
Nat Prod Res ; 34(4): 558-562, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30388891

RESUMO

Methanol extract of Indigofera hirsuta, was evaluated for its antiradical potential and capacity in inhibiting lipoxygenase and aldose/aldehyde reductase enzymes. The ethyl acetate fraction derived from the methanol extract partition, showed the greatest antioxidant capacity, while the butanol was the strongest inhibitor of lipoxygenase enzyme. All fractions (diethyl ether, ethyl acetate, butanol and the aqueous residue) exhibited strong inhibition capacity of both aldose/aldehyde reductase enzymes, which comes in agreement with the ethnomedicinal plant utilization as an antidiabetic agent. LC-DAD-MS(ESI+) fraction analysis verified the findings above, leading to a conclusion regarding the biological activities attributed to the main compounds. Phytochemical analysis led to the identification of an indolic dimer, cinnamic acids, phenolics, flavonoid glycosides, a cyclic polyol, the rare sugar 1-methyl-ß-D-glucopyranoside and glycerol. Many of these compounds were isolated for the first time in Indigofera species while the indolic dimer was isolated for the first time in the Fabaceae family.


Assuntos
Antioxidantes/isolamento & purificação , Inibidores Enzimáticos/isolamento & purificação , Indigofera/química , Compostos Fitoquímicos/análise , Extratos Vegetais/química , Aldeído Redutase/antagonistas & inibidores , Antioxidantes/química , Antioxidantes/farmacologia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Flavonoides/análise , Glicosídeos/análise , Humanos , Inibidores de Lipoxigenase , Fenóis/análise , Fenóis/química , Compostos Fitoquímicos/isolamento & purificação , Componentes Aéreos da Planta/química
15.
Nat Prod Res ; 34(3): 323-328, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30580630

RESUMO

Chemical investigation of Dendrobium plicatile Lindl resulted in the isolation and identification of one new bibenzyl, 2-chloro-3, 4'-dihydroxy-3',5-dimethoxybibenzyl (1), as well as 15 known stilbenoids. The structures of this new compound was elucidated by extensive spectroscopic analysis, including HRESIMS, 1H and 13C NMR, DEPT, HMBC, COSY, HMQC, NOESY. Compounds 2, 3 and 5 were obtained from this genus for the first time, compounds 8, 10, 13 and 14 were obtained from this plant for the first time. In addition, the new compound exhibited potent cytotoxic activities against the human breast cancer (MDA-MB231) cell line, the hepatocellular carcinoma (HepG2) cell line and the human lung carcinoma (A549) cell line, with IC50 3.41, 3.02, 2.80 µM, respectively.


Assuntos
Dendrobium/química , Componentes Aéreos da Planta/química , Estilbenos/isolamento & purificação , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Citotoxinas/isolamento & purificação , Citotoxinas/farmacologia , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Análise Espectral/métodos , Estilbenos/química , Estilbenos/farmacologia
16.
Phytochemistry ; 169: 112161, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31600653

RESUMO

A phytochemical investigation on the MeOH extract of the twigs and needles of the endangered plant Picea brachytyla led to the isolation and characterization of thirty-eight structurally diverse terpenoids. Seven of these molecules are previously undescribed, including three abietane-type (brachytylins A-C) and one labdane-type (brachytylin D) diterpenoids, an unseparated C-24 epimeric mixture of cycloartane-type triterpenoids (brachytylins E/F, ratio: 1:1), and a rare rearranged 12(1 → 6)-abeo-megastigmane glycoside (brachytylins G). Their structures and absolute configurations were determined by extensive spectroscopic (e.g., detailed 2D NMR and ECD) methods and/or X-ray diffraction analyses. All the isolates were evaluated for their inhibitory activities against the adenosine triphosphate (ATP)-citrate lyase (ACL) and the Src homology-2 domain containing protein tyrosine phosphatase-2 (SHP2). Among them, abiesadine J showed inhibitory effect against ACL, displaying an IC50 value of 17 µM. 3S,23R-Dihydroxycycloart-24-en-26-oic acid exhibited inhibitory effect on SHP2, with an IC50 value of 19 µM. Meanwhile, 3R*,23S*-dihydroxycycloart-24-en-26-oic acid was found to have inhibitory effects against both ACL and SHP2, with IC50 values of 16 and 12 µM, respectively.


Assuntos
ATP Citrato (pro-S)-Liase/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Compostos Fitoquímicos/farmacologia , Picea/química , Componentes Aéreos da Planta/química , Proteína Tirosina Fosfatase não Receptora Tipo 11/antagonistas & inibidores , Terpenos/farmacologia , ATP Citrato (pro-S)-Liase/metabolismo , China , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Humanos , Modelos Moleculares , Conformação Molecular , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação , Proteína Tirosina Fosfatase não Receptora Tipo 11/metabolismo , Relação Estrutura-Atividade , Terpenos/química , Terpenos/isolamento & purificação
17.
Talanta ; 206: 120248, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514863

RESUMO

Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIR-based metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed.


Assuntos
Asteraceae/química , Citotoxinas/análise , Lactonas/análise , Metabolômica/métodos , Sesquiterpenos de Guaiano/análise , Linhagem Celular Tumoral , Cromatografia , Citotoxinas/isolamento & purificação , Citotoxinas/toxicidade , Humanos , Lactonas/isolamento & purificação , Lactonas/toxicidade , Espectroscopia de Ressonância Magnética , Componentes Aéreos da Planta/química , Sesquiterpenos de Guaiano/isolamento & purificação , Sesquiterpenos de Guaiano/toxicidade , Espectroscopia de Infravermelho com Transformada de Fourier
18.
J Pharm Biomed Anal ; 177: 112843, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31509788

RESUMO

An UHPLC method was developed for the determination of 15 prenylflavonoids from aerial parts of Epimedium grandiflorum and related species (Berberidaceae). The separation was achieved using a reverse phased column and water/acetonitrile gradient as a mobile phase at a temperature of 40°C. The developed analytical method was validated for linearity, limits of detection (LOD) and limits of quantification (LOQ), stability and repeatability. The LOD and LOQ were found to be in the range from 0.1-0.5 µg/mL and 0.3-1 µg/mL, respectively. The wavelength used for quantification with the photodiode array detector was 269 nm. The total content of 15 prenylflavonoids was 9.1-20.6 mg/g for E. grandiflorum (except for sample #2899 and #20862), 5.6-35.4 mg/g for E. brevicornu and 10.8-30.5 mg/g for E. sagittatum. Twenty dietary supplements contained in the range from 0.1 to 81.7 mg/day. The developed method is simple, rapid and especially suitable for quality assessment of E. grandiflorum and dietary supplements containing E. grandiflorum. Liquid chromatography quadrupole time-of-flight-mass spectrometry (LC-QToF) is described for the identification and confirmation of compounds in plant samples and dietary supplements. This technique is also used for chemical profiling of Epimedium samples. This method involved the use of protonated ions in the positive ion mode and deprotonated ions in the negative ion mode with extracted ion chromatogram (EIC). Chemometric analytical tools for visualizing the plant and commercial samples quality were used for discriminating between Epimedium species and dietary supplements with regards to the relative content or presence of components. A HPTLC method was also developed for the fast chemical fingerprint analysis of Epimedium species.


Assuntos
Suplementos Nutricionais/análise , Epimedium/química , Flavonoides/análise , Controle de Qualidade , Cromatografia Líquida de Alta Pressão/métodos , Suplementos Nutricionais/normas , Estudos de Viabilidade , Flavonoides/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Limite de Detecção , Componentes Aéreos da Planta/química , Reprodutibilidade dos Testes , Espectrometria de Massas por Ionização por Electrospray/métodos
19.
J Ethnopharmacol ; 247: 112281, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31600559

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Artemisia annua L. is a heat-clearing Chinese medicine and well-known for its antimalarial constituent, artemisinin. It has gained increasing attention for its anti-inflammatory and immunoregulatory activities. Interestingly, the crude polysaccahrides of A. annua exhibited potent anticomplement activity. This study was to isolate and characterize its anticomplement homogeneous polysaccharides from A. annua, and reveal the relationship between structures and anticomplement activities of the isolated polysaccharides. MATERIALS AND METHODS: Water-soluble crude polysaccharides from the aerial parts of A. annua were extracted and fractionated by DEAE-cellulose and Sephacryl S-300 gel permeation chromatography. Homogeneity, molecular weight, monosaccharide composition, methylation and NMR analysis were performed to characterize the structures of homogeneous polysaccharides. Their anticomplement activities and targeting components in the complement activation cascade were evaluated by hemolytic assays. RESULTS: Three homogeneous polysaccharides (AAP01-1, AAP01-2 and AAP01-3) were obtained from A. annua. AAP01-1 was composed of seven monosaccharides, including mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose. AAP01-2 and AAP01-3 had similar monosaccharides with AAP01-1, except the absence of glucuronic acid. They were all branched acidic heteropolysaccharides with different contents of galacturonic acid (8%, 28% and 15% for AAP01-1, AAP01-2 and AAP01-3, respectively). AAP01-2 showed potent anticomplement activity with CH50 value of 0.360 ±â€¯0.020 mg/mL through the classical pathway and AP50 value of 0.547 ±â€¯0.033 mg/mL through the alternative pathway. AAP01-3 exhibited slightly weaker activity (CH50: 1.120 ±â€¯0.052 mg/mL, AP50: 1.283 ±â€¯0.061 mg/mL), while AAP01-1 was inactive. Moreover, AAP01-2 acted on C1q, C3, C4, C5 and C9 components and AAP01-3 interacted with C3, C4 and C5 components in the activation cascade of complement system. CONCLUSION: These results indicated that the relatively high contents of galacturonic acid were important for anticomplement activities of the polysaccharides from A. annua. The anticomplement polysaccharides are another kind of bioactive constituents conferring heat-clearing effects of A. annua.


Assuntos
Artemisia annua/química , Ativação do Complemento/efeitos dos fármacos , Inativadores do Complemento/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Polissacarídeos/farmacologia , Animais , Bioensaio , Inativadores do Complemento/química , Inativadores do Complemento/isolamento & purificação , Proteínas do Sistema Complemento , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Cobaias , Hemólise/efeitos dos fármacos , Ácidos Hexurônicos/química , Ácidos Hexurônicos/isolamento & purificação , Ácidos Hexurônicos/farmacologia , Modelos Animais , Estrutura Molecular , Componentes Aéreos da Planta/química , Coelhos , Relação Estrutura-Atividade
20.
Chin J Nat Med ; 17(12): 924-927, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31882047

RESUMO

A new sesquiterpenoid and two pregnane steroids, named vernobockolide C (1) and vernobockones A and B (2 and 3), respevtively, along with a known sesquiterpenoid, 7, 10-epoxy-11-hydroxy-bisabol-2-en-15-al, were isolated from the aerial part of Vernonia bockiana. Their structures were elucidated on the basis of extensive spectroscopic data analysis, especially 2D NMR (HSQC, HMBC, and ROESY). This study further expands the chemical space of this underexplored species.


Assuntos
Sesquiterpenos/química , Sesquiterpenos/isolamento & purificação , Esteroides/química , Esteroides/isolamento & purificação , Vernonia/química , China , Espectroscopia de Ressonância Magnética , Componentes Aéreos da Planta/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA