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1.
Braz Oral Res ; 33: e117, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31939498

RESUMO

The aim of this study was to evaluate the effect of mineral trioxide aggregate (MTA) and Brazilian propolis on the cell viability, mineralization, anti-inflammatory ability, and migration of human dental pulp cells (hDPCs). The cell viability was evaluated with CCK-8 kit after 1, 5, 7, and 9 days. The deposition of calcified matrix and the expression of osteogenesis-related genes were evaluated by Alizarin Red staining and real-time PCR after incubation in osteogenic medium for 21 days. The expression of inflammation-related genes in cells was determined after exposure to 1 µg/mL LPS for 3 h. Finally, the numbers of cells that migrated through the permeable membranes were compared during 15 h. Propolis and MTA significantly increased the viability of hDPCscompared to the control group on days 7 and 9. In the propolis group, significant enhancement of osteogenic potential and suppressed expression of IL-1ß and IL-6 was observed after LPS exposure compared to the MTA and control groups. The number of migration cells in the propolis group was similar to that of the control group, while MTA significantly promoted cell migration. Propolis showed comparable cell viability to that of MTA and exhibited significantly higher anti-inflammatory and mineralization promotion effects on hDPCs.


Assuntos
Compostos de Alumínio/farmacologia , Anti-Inflamatórios/farmacologia , Compostos de Cálcio/farmacologia , Polpa Dentária/citologia , Polpa Dentária/efeitos dos fármacos , Óxidos/farmacologia , Própole/farmacologia , Silicatos/farmacologia , Antraquinonas , Brasil , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Combinação de Medicamentos , Humanos , Interleucina-1beta/análise , Interleucina-6/análise , Odontoblastos/efeitos dos fármacos , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes , Estatísticas não Paramétricas , Fator de Necrose Tumoral alfa/análise
2.
J Appl Oral Sci ; 28: e20190023, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31800871

RESUMO

When exposure of the pulp to external environment occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain pulp tissue vitality and function. These clinical situations require the use of materials that induce dentin repair and, subsequently, formation of a mineralized tissue. OBJECTIVE: This work aims to assess the effect of tricalcium silicate cements and mineral trioxide aggregate cements, including repairing dentin formation and inflammatory reactions over time after pulp exposure in Wistar rats. METHODOLOGY: These two biomaterials were compared with positive control groups (open cavity with pulp tissue exposure) and negative control groups (no intervention). The evaluations were performed in three stages; three, seven and twenty-one days, and consisted of an imaging (nuclear medicine) and histological evaluation (H&E staining, immunohistochemistry and Alizarin Red S). RESULTS: The therapeutic effect of these biomaterials was confirmed. Nuclear medicine evaluation demonstrated that the uptake of 99mTc-Hydroxymethylene diphosphonate (HMDP) showed no significant differences between the different experimental groups and the control, revealing the non-occurrence of differences in the phosphocalcium metabolism. The histological study demonstrated that in mineral trioxide aggregate therapies, the presence of moderate inflammatory infiltration was found after three days, decreasing during follow-ups. The formation of mineralized tissue was only verified at 21 days of follow-up. The tricalcium silicate therapies demonstrated the presence of a slight inflammatory infiltration on the third day, increasing throughout the follow-up. The formation of mineralized tissue was observed in the seventh follow-up day, increasing over time. CONCLUSIONS: The mineral trioxide aggregate (WhiteProRoot®MTA) and tricalcium silicate (Biodentine™) present slight and reversible inflammatory signs in the pulp tissue, with the formation of mineralized tissue. However, the exacerbated induction of mineralized tissue formation with the tricalcium silicate biomaterial may lead to the formation of pulp calcifications.


Assuntos
Compostos de Alumínio/farmacologia , Materiais Biocompatíveis/farmacologia , Compostos de Cálcio/farmacologia , Polpa Dentária/efeitos dos fármacos , Dentina/efeitos dos fármacos , Dentinogênese/efeitos dos fármacos , Óxidos/farmacologia , Silicatos/farmacologia , Animais , Polpa Dentária/patologia , Capeamento da Polpa Dentária/métodos , Exposição da Polpa Dentária/tratamento farmacológico , Exposição da Polpa Dentária/patologia , Combinação de Medicamentos , Proteínas da Matriz Extracelular/análise , Imuno-Histoquímica , Masculino , Imagem Molecular/métodos , Odontoblastos/efeitos dos fármacos , Fosfoproteínas/análise , Agentes de Capeamento da Polpa Dentária e Pulpectomia/farmacologia , Pulpite/tratamento farmacológico , Pulpite/patologia , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Sialoglicoproteínas/análise , Fatores de Tempo
3.
J Appl Oral Sci ; 28: e20190105, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31800873

RESUMO

Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. OBJECTIVE: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. METHODOLOGY: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). RESULTS: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. CONCLUSION: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair.


Assuntos
Compostos de Alumínio/química , Compostos de Alumínio/farmacologia , Compostos de Cálcio/química , Compostos de Cálcio/farmacologia , Cimentos Dentários/química , Cimentos Dentários/farmacologia , Polpa Dentária/citologia , Polpa Dentária/efeitos dos fármacos , Fosfatase Alcalina/análise , Fosfatase Alcalina/efeitos dos fármacos , Animais , Bismuto/química , Bismuto/farmacologia , Cloreto de Cálcio/química , Cloreto de Cálcio/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Combinação de Medicamentos , Expressão Gênica/efeitos dos fármacos , Teste de Materiais , Camundongos , Odontoblastos/efeitos dos fármacos , Óxidos/química , Óxidos/farmacologia , Reprodutibilidade dos Testes , Silicatos/química , Silicatos/farmacologia , Fatores de Tempo , Óxido de Zinco/química , Óxido de Zinco/farmacologia
4.
J Appl Oral Sci ; 27: e20180693, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31596370

RESUMO

OBJECTIVES: To compare the sealing ability and biocompatibility of Biodentine with mineral trioxide aggregate (MTA) when used as root-end filling materials. METHODOLOGY: The Cell Counting Kit-8 (CCK-8) assay was used to compare the cytotoxicity of MTA and Biodentine. Twenty-one extracted teeth with a single canal were immersed in an acidic silver nitrate solution after root-end filling. Then, the volume and depth of silver nitrate that infiltrated the apical portion of the teeth were analyzed using micro-computed tomography (micro-CT). Seventy-two roots from 3 female beagle dogs were randomly distributed into 3 groups and apical surgery was performed. After six months, the volume of the bone defect surrounding these roots was analyzed using micro-CT. RESULTS: Based on the results of the CCK-8 assay, MTA and Biodentine did not show statistically significant differences in cytotoxicity (P>0.05). The volume and the depth of the infiltrated nitrate solution were greater in the MTA group than in the Biodentine group (P<0.05). The volume of the bone defect was larger in the MTA group than in the Biodentine group. However, the difference was not significant (P>0.05). The volumes of the bone defects in the MTA and Biodentine groups were smaller than the group without any filling materials (P<0.05). CONCLUSIONS: MTA and Biodentine exhibited comparable cellular biocompatibility. Biodentine showed a superior sealing ability to MTA in root-end filling. Both Biodentine and MTA promoted periradicular bone healing in beagle dog periradicular surgery models.


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Óxidos/farmacologia , Tecido Periapical/efeitos dos fármacos , Ligamento Periodontal/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/farmacologia , Tratamento do Canal Radicular/métodos , Silicatos/farmacologia , Cicatrização/efeitos dos fármacos , Adolescente , Animais , Regeneração Óssea/efeitos dos fármacos , Contagem de Células , Células Cultivadas , Cães , Combinação de Medicamentos , Humanos , Masculino , Teste de Materiais , Osteogênese/efeitos dos fármacos , Tecido Periapical/citologia , Tecido Periapical/diagnóstico por imagem , Ligamento Periodontal/diagnóstico por imagem , Reprodutibilidade dos Testes , Fatores de Tempo , Raiz Dentária/diagnóstico por imagem , Raiz Dentária/efeitos dos fármacos , Raiz Dentária/cirurgia , Resultado do Tratamento , Microtomografia por Raio-X , Adulto Jovem
5.
Braz Oral Res ; 33: e060, 2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31365705

RESUMO

This study evaluated the effect of hypertension on tissue response and biomineralization capacity of white Mineral Trioxide Aggregate (MTA), High-plasticity MTA (MTA HP), and Biodentine® (BDT) in rats. Polyethylene tubes filled with MTA, MTA HP, BDT, and the control group (empty tubes) were placed into the dorsal subcutaneous tissue of 32 male rats (16 normotensive (NT) and 16 hypertensive rats - 8 per group). After 7 and 30 days, the polyethylene tubes surrounded by connective tissue were removed, fixed, and embedded in histological resin. The mean number of inflammatory cells was estimated in HE-stained sections, biomineralization was quantified as area (µm2) by Kossa (VK) staining, and examination by polarized light (LP) microscopy was performed. The differences amongst the groups were analyzed statistically by the Mann-Whitney or Student's t test, according to Shapiro-Wilk test of normality (p < 0.05). The inflammatory responses to all materials were greater in hypertensive rats than in NT rats (p < 0.05). Positive VK staining in MTA and BDT were more pronounced in NT rats at 7 and 30 days (p < 0.05). Birefringent structures in LP for MTA, MTA HP, and BDT were more pronounced in NT rats at 7 days (p<0.05). In rats, hypertension was able to increase inflammatory infiltrate and decrease biomineralization of the tested materials.


Assuntos
Compostos de Alumínio/farmacologia , Materiais Biocompatíveis/farmacologia , Biomineralização/fisiologia , Compostos de Cálcio/farmacologia , Hipertensão/fisiopatologia , Óxidos/farmacologia , Silicatos/farmacologia , Tela Subcutânea/efeitos dos fármacos , Tela Subcutânea/fisiopatologia , Animais , Combinação de Medicamentos , Hipertensão/complicações , Inflamação/patologia , Inflamação/fisiopatologia , Masculino , Teste de Materiais , Microscopia de Polarização , Ratos Wistar , Reprodutibilidade dos Testes , Tela Subcutânea/patologia , Fatores de Tempo
6.
J Appl Oral Sci ; 27: e20180550, 2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31365709

RESUMO

PURPOSE: To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). METHODOLOGY: Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). RESULTS: The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. CONCLUSION: The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA.


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Compostos Férricos/farmacologia , Inflamação/imunologia , Interleucina-6/análise , Óxidos/farmacologia , Pulpotomia/efeitos adversos , Silicatos/farmacologia , Animais , Polpa Dentária/efeitos dos fármacos , Polpa Dentária/patologia , Combinação de Medicamentos , Masculino , Ratos Wistar , Estatísticas não Paramétricas , Fatores de Tempo
7.
J Endod ; 45(7): 907-916, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31126710

RESUMO

INTRODUCTION: The purpose of this study was to verify the expression of the calcium-sensing receptor (CaSR) and its role in mineral trioxide aggregate (MTA)-induced odontoblastic differentiation and mineralization in human dental pulp cells (hDPCs). METHODS: The expression of CaSR in human dental pulp tissue and hDPCs was detected using immunohistochemical and immunofluorescent assays. Then, hDPCs were cultured in specific medium supplemented with defined concentrations of MTA dilute alone or in combination with calcimimetic R-568 (a positive allosteric modulator of CaSR [Tocris Bioscience, Bristol, UK]), and cell viability was monitored by Cell Counting Kit-8 (Dojindo Molecular Technologies, Kumamoto, Japan) analysis. Alkaline phosphatase activity, alizarin red S staining, quantitative real-time polymerase chain reaction, and Western blot were used to investigate the gene/protein expression of odontoblastic-associated markers and CaSR in medium supplemented with different combinations of diluted MTA, R-568, and calcilytic Calhex 231 (a negative allosteric modulator of CaSR [Sigma-Aldrich, St Louis, MO]). RESULTS: CaSR was slightly expressed in the central pulp tissue, whereas it was strongly expressed in the odontoblast layer, plasma membrane, and cytoplasm of hDPCs. Cell Counting Kit-8 assay indicated maximum cell viability in cultures treated with 1:8 diluted MTA additives. Compared with undifferentiated controls, the cells at the early stage of odontoblastic differentiation exhibited lower CaSR protein expression. The combination of 1:8 diluted MTA with 0.1 and 1.0 µmol/L R-568 led to significantly increased cell vitality but decreased alkaline phosphatase activity and mineralized deposit formation, and this negative effect could be attenuated by 1.0 µmol/L Calhex 231 supplementation. Quantitative polymerase chain reaction results showed a significant up-regulation of RUNX2, DSPP, DMP-1, and OCN gene expression in the 1 µmol/L R-568-treated hDPCs. Western blot analysis indicated that the treatment by MTA and R-568 alone or their combination gave no clear trend on the protein levels of CaSR and dentin sialophosphoprotein, whereas Calhex 231 can increase their expressions. In addition, the up-regulation of Akt phosphorylation was observed in R-568- and Calhex 231-treated hDPCs. CONCLUSIONS: Our data indicated that CaSR is expressed in human dental pulp and hDPCs and that it can negatively or positively regulate MTA-induced mineralization of hDPCs via the phosphoinositide 3-kinase/Akt pathway in a ligand-dependent manner, suggesting a therapeutic target for modulating reparative dentin formation.


Assuntos
Compostos de Alumínio , Compostos de Cálcio , Diferenciação Celular , Polpa Dentária , Odontoblastos , Óxidos , Receptores de Detecção de Cálcio , Silicatos , Fosfatase Alcalina , Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Proliferação de Células , Células Cultivadas , Combinação de Medicamentos , Proteínas da Matriz Extracelular , Humanos , Óxidos/farmacologia , Fosfatidilinositol 3-Quinases , Receptores de Detecção de Cálcio/fisiologia , Silicatos/farmacologia
8.
Indian J Dent Res ; 30(1): 31-36, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30900653

RESUMO

Context: It is important to develop new therapeutic materials that have requisite clinical actions, are safe and economical. Aims: This study aims to histologically evaluate curcumin, an extract of turmeric (Curcuma longa) as a pulpotomy agent in rat molars and to compare it to mineral trioxide aggregate (MTA). Settings and Design: Animal study. Subjects and Methods: Twelve Wistar-Albino rats were randomly divided into two groups of 6 each. Pulpotomies were performed on caries free maxillary first and second molars on both sides of the arch, with MTA and curcumin (24 teeth each), respectively. Access cavities were sealed with resin-modified glass ionomer cement. Postoperative histological evaluation of pulpotomized teeth in both groups was done at 7, 14, and 30 days under a light microscope (×10). Statistical Analysis Used: Data were evaluated with Freidman's test and Mann-Whitney test at 0.05 level. Results: (a) There was a gradual reduction in inflammatory cell response in both groups across time periods tested (MTA P = 0.074, curcumin P = 0.039). (b) The overall architecture of pulp was maintained better in the curcumin group across all time periods tested (P = 0.368). (c) Dentinal bridge formation was consistently seen across time periods tested in MTA group (P = 0.9094) and was feeble in curcumin group (P = 0.9094) across time periods tested. Conclusions: Curcumin has been shown to have wound healing properties. It has the potential to be developed into a predictable and cost-effective vital pulp therapy medicament.


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Curcumina/farmacologia , Polpa Dentária/patologia , Polpa Dentária/fisiologia , Óxidos/farmacologia , Agentes de Capeamento da Polpa Dentária e Pulpectomia/farmacologia , Pulpotomia/métodos , Silicatos/farmacologia , Cicatrização/efeitos dos fármacos , Compostos de Alumínio/administração & dosagem , Animais , Compostos de Cálcio/administração & dosagem , Curcumina/administração & dosagem , Combinação de Medicamentos , Óxidos/administração & dosagem , Ratos Wistar , Silicatos/administração & dosagem
9.
Chemosphere ; 223: 91-98, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30771652

RESUMO

Ionic zinc is considered as an environmental pollutant. This work systematically investigated leaching mechanisms of calcium sulfoaluminate cement (CSA) pastes incorporated with/without ZnCl2 under the attacks of chloride and/or sulfate. The leaching behaviors of CSA pastes in the leaching solution are in-situ and continuously monitored by innovative non-contact electrical impedance measurement (NCEIM) and pH meter. The dissolution and diffusion during the leaching process are experimentally identified. Other techniques are also performed to verify the finding of NCEIM: the ion chromatograph and inductively coupled plasma optical emission spectrometer reveal the leaching or decomposition sequence of CSA pastes during the leaching process. Besides, results from XRD and SEM techniques demonstrate that main solid products in CSA pastes are ettringite and calcium monosulfoaluminate hydrates. The incorporation of Zn in the pastes has great impact on the decomposition of CSA pastes in the temperature elevation. External chloride and/or sulfate attacks significantly alter the pore structure of CSA pastes during the leaching process.


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Cloretos/farmacologia , Materiais de Construção/efeitos adversos , Compostos de Enxofre/farmacologia , Poluição da Água , Compostos de Zinco/farmacologia , Difusão , Poluição Ambiental , Porosidade , Solubilidade , Sulfatos/farmacologia
10.
Int J Pharm Compd ; 23(1): 23-31, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30668532

RESUMO

Primary focal hyperhidrosis is idiopathic, localized, uncontrollable, excessive, and unpredictable sweating beyond what is necessary to regulate body temperature. Primary hyperhidrosis is thought to affect approximately 2% to 3% of the population, and its effect on a patient's quality of life is very significant. Primary focal hyperhidrosis can be managed using various therapeutic options, including drugs (topical and systemic), nonsurgical interventions (e.g., iontophoresis, botulinum toxin injections), and surgery. This article, which is presented in 2 parts, is a comprehensive review of the topical, evidence-based treatments of primary focal hyperhidrosis, and it covers the following active pharmaceutical ingredients: aluminum salts, methenamine, glycopyrronium salts, oxybutynin chloride; the latter 2 ingredients will be discussed in part 2 of this article. This article discusses the evidence-based data that exists from clinical trials that support the use of topical medications to treat the pathology from efficacy and from a safety point of view. This review also discusses compounding considerations for professionally and safely compounding various topical preparations. In addition, a range of relevant formulas are attached to the article and can be used by compounding pharmacists.


Assuntos
Hiperidrose , Iontoforese/métodos , Qualidade de Vida , Administração Tópica , Compostos de Alumínio/química , Compostos de Alumínio/farmacologia , Humanos
11.
J Appl Oral Sci ; 27: e20180247, 2019 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-30624470

RESUMO

OBJECTIVE: This study aimed to investigate the effects of dodecacalcium hepta-aluminate (C12A7) content on some physicochemical properties and cytocompatibility of tricalcium silicate (C3S) cement using human dental pulp cells (hDPCs). MATERIAL AND METHODS: High purity C3S cement was manufactured by a solid phase method. C12A7 was mixed with the cement in proportions of 0, 5, 8, and 10 wt% (C12A7-0, -5, -8, and -10, respectively). Physicochemical properties including initial setting time, compressive strength, and alkalinity were evaluated. Cytocompatibility was assessed with cell viability tests and cell number counts. Statistical analysis was performed by using one-way analysis of variance (ANOVA) and Tukey's test (p<0.05). RESULTS: The initial setting time of C3S-based cement was shorter in the presence of C12A7 (p<0.05). After 1 day, C12A7-5 showed significantly higher compressive strength than the other groups (p<0.05). After 7 days, the compressive strength of C12A7-5 was similar to that of C12A7-0, whereas other groups showed strength lower than C12A7-0. The pH values of all tested groups showed no significant differences after 1 day (p>0.05). The C12A7-5 group showed similar cell viability to the C12A7-0 group (p>0.05), while the other experimental groups showed lower values compared to C12A7-0 group (p<0.05). The number of cells grown on the C12A7-5 specimen was higher than that on C12A7-8 and -10 (p<0.05). CONCLUSIONS: The addition of C12A7 to C3S cement at a proportion of 5% resulted in rapid initial setting time and higher compressive strength with no adverse effects on cytocompatibility.


Assuntos
Compostos de Alumínio/química , Compostos de Cálcio/química , Cimentos Dentários/química , Cavidade Pulpar/citologia , Silicatos/química , Compostos de Alumínio/farmacologia , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Compostos de Cálcio/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Força Compressiva , Cimentos Dentários/farmacologia , Cavidade Pulpar/efeitos dos fármacos , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Tamanho da Partícula , Valores de Referência , Reprodutibilidade dos Testes , Silicatos/farmacologia , Fatores de Tempo , Difração de Raios X
12.
Med Princ Pract ; 28(1): 36-40, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30257258

RESUMO

OBJECTIVE: This study compares the effect of the white mineral trioxide aggregate (WMTA) accelerators, including disodium hydrogen orthophosphate (Na2HPO4; 2.5 wt%), calcium chloride (CaCl2; 5 and 10 wt%), and KY jelly, on the push-out bond strength of WMTA. The null hypothesis was that the WMTA accelerators would not affect the push-out bond strength of WMTA. MATERIALS AND METHODS: Slices (2-mm-thick) were obtained from 75 human mandibular molar distal roots. The slices were enlarged up to size 6 Gates-Glidden burs to obtain a 1.5-mm canal diameter. The slices were randomly divided into 4 experimental groups and a control group (n = 15 in each group). Freshly prepared WMTA mixture was placed into the root slices and stored at 37°C in a 100% humidified atmosphere for 60 days. The force required to dislodge the WMTA cement from the root slice was determined using a universal testing machine. The push-out bond strength was calculated. RESULTS: Push- out bond strength of 5- and 10-wt% CaCl2, and 2.5-wt% Na2HPO4 WMTA groups was significantly lower than in the KY-jelly and control groups (p < 0.05). The mean push-out bond strength of KY jelly was lower than in the control group but not statistically significant. CONCLUSION: The addition of KY jelly to WMTA did not have an adverse effect on the push-out bond strength of WMTA, in contrast to the other accelerators, including Na2HPO4 and CaCl2, which reduced the push-out bond strength.


Assuntos
Compostos de Alumínio/farmacologia , Cloreto de Cálcio/farmacologia , Compostos de Cálcio/farmacologia , Celulose/análogos & derivados , Colagem Dentária/métodos , Glicerol/farmacologia , Óxidos/farmacologia , Fosfatos/farmacologia , Propilenoglicóis/farmacologia , Silicatos/farmacologia , Celulose/farmacologia , Análise do Estresse Dentário , Combinação de Medicamentos , Humanos , Distribuição Aleatória , Raiz Dentária
13.
Clin Oral Investig ; 23(1): 169-177, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29572687

RESUMO

OBJECTIVE: Evaluate, in vivo, the biocompatibility via subcutaneous inflammatory tissue response and mineralization ability of the new MTA Flow compared to MTA Angelus and ProRoot MTA. MATERIALS AND METHODS: Forty male Wistar rats were assigned and received subcutaneous polyethylene tube implants containing the test materials and a control group with empty tube (n = 10 animals/group). After days 7, 15, 30, and 60, the animals were euthanized and the polyethylene tubes were removed with the surrounding tissues. Inflammatory infiltrate and thickness of the fibrous capsule were histologically evaluated. Mineralization was analyzed by Von Kossa staining and under polarized light. Data were analyzed via Kruskal-Wallis and Dunn's test with a significance level of 5%. RESULTS: MTA Angelus induced the mildest reaction after 7 (P > .05) and 15 days (P < .05) followed by MTA Flow, both cements achieving mild inflammatory reaction after 15 days. ProRoot MTA induced a severe inflammation on day 7 and was reducing after day 15 (P > .05). No difference was observed after days 30 or 60 (P > .05). Von Kossa staining and birefringent structures were positive to all materials. CONCLUSIONS: At the end of the experiment, the novel MTA Flow showed biocompatibility and induced biomineralization in all time periods. CLINICAL RELEVANCE: The final consistence obtained in MTA Flow may facilitate several procedures, indicating that the MTA Flow has a promising application in endodontics.


Assuntos
Compostos de Alumínio/farmacologia , Materiais Biocompatíveis/farmacologia , Biomineralização , Compostos de Cálcio/farmacologia , Óxidos/farmacologia , Silicatos/farmacologia , Tela Subcutânea/efeitos dos fármacos , Animais , Bismuto , Combinação de Medicamentos , Implantes de Medicamento , Masculino , Ratos , Ratos Wistar , Materiais Restauradores do Canal Radicular
14.
Int Endod J ; 52(2): 193-200, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30035812

RESUMO

AIM: To evaluate the influence of powder-to-gel ratio (0.19 g powder to 50 µL of gel, thick MTA Flow, and 0.06 g powder to 50 µL of gel, fluid MTA Flow) on biocompatibility of MTA Flow (Ultradent Products Inc., South Jordan, UT, USA, lot: 2015122901) and compare it with Biodentine (Septodont Inc., Saint-Maur-des-Fossés, France, lot: B18542A). METHODOLOGY: The materials were manipulated and inserted into polyethylene tubes for implantation in twenty rats. After 7, 15, 30 and 60 days, the specimens were removed and embedded in paraffin. Haematoxylin and eosin sections were used to count the number of inflammatory cells (IC) and fibroblasts mm-2 (Fb). In the Masson's trichrome-stained sections, the fibrous capsule thickness was measured; picrosirius red-stained sections were used for birefringent collagen quantification. The data were submitted to two-way ANOVA and Tukey test (P ≤ 0.05). RESULTS: A significantly lower number of IC and consequently higher number of Fb were observed in the capsules adjacent to thick MTA Flow at all periods, in comparison with other materials (P ≤ 0.05). At 60 days, the quantity of birefringent collagen was significantly greater in the tissue in contact with thick MTA Flow, when compared with fluid MTA Flow and Biodentine. CONCLUSIONS: Although thick MTA Flow induced a less intense inflammatory response, all evaluated materials are biocompatible because they allowed regression of this process after 60 days.


Assuntos
Compostos de Alumínio/farmacologia , Materiais Biocompatíveis/farmacologia , Compostos de Cálcio/farmacologia , Teste de Materiais , Óxidos/farmacologia , Silicatos/farmacologia , Análise de Variância , Animais , Colágeno , Combinação de Medicamentos , Fibroblastos/efeitos dos fármacos , Fibroblastos/patologia , Masculino , Modelos Animais , Agentes de Capeamento da Polpa Dentária e Pulpectomia/farmacologia , Ratos , Materiais Restauradores do Canal Radicular/farmacologia , Fatores de Tempo
15.
Odontology ; 107(1): 54-63, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30039235

RESUMO

The aim of this study was to evaluate the biocompatibility of two comparatively new calcium silicate containing sealers (MTA-Fillapex and BioRoot-RCS) with that of two established sealers (AH-Plus, epoxy resin-based; Pulp-Canal-Sealer, zinc oxide eugenol containing). Human periodontal ligament cells (PDL-cells) were brought in contact with eluates from freshly mixed and set sealer. The sealers were mixed strictly according to the manufacturers' instructions and identically samples were produced. 1:1, 1:2, and 1:10 dilutions of sealers extract were used. Extracts from freshly mixed sealer were added to the PDL-cells on day one to simulate a clinical scenario. Subsequently, at 24 h, 7, 14, and 21 days extracts form set sealers were used for PDL-cell culturing. PDL-cell viability was analyzed by living-cell-count, MTT-assay, and living/dead-staining, cytotoxicity by LDH-assay, and changes by Richardson-staining. All data were statistically evaluated by one way ANOVA and a posthoc analysis with Bonferroni-Holm testing (p < 0.05). In contact with BioRoot-RCS a regeneration of the PDL-cells were observed over time. This sealer showed the lowest toxicity in a freshly mixed and set state (p < 0.05). MTA-Fillapex and Pulp-Canal-Sealer were cytotoxic in a fresh as well as in a set state, whereas AH-Plus was cytotoxic in a freshly mixed state, but not when the sealer was set. BioRoot-RCS is biocompatible and bioactive because it seems to have a positive influence on the PDL-cell metabolism. Pulp Canal Sealer and MTA-Fillapex showed no biocompatibility in contact with PDL-cells at all. Freshly mixed AH Plus is less biocompatible on PDL than in a set state.


Assuntos
Ligamento Periodontal/citologia , Materiais Restauradores do Canal Radicular/farmacologia , Compostos de Alumínio/farmacologia , Materiais Biocompatíveis , Compostos de Cálcio/farmacologia , Sobrevivência Celular , Células Cultivadas , Combinação de Medicamentos , Resinas Epóxi/farmacologia , Humanos , Técnicas In Vitro , Teste de Materiais , Dente Serotino , Óxidos/farmacologia , Cimento de Policarboxilato/farmacologia , Povidona/farmacologia , Silicatos/farmacologia , Cimento de Óxido de Zinco e Eugenol/farmacologia
16.
Clin Oral Investig ; 23(3): 1237-1252, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29982967

RESUMO

OBJECTIVES: Evaluate the tissue reaction of periodontium subjacent to furcation perforations in rat molars sealed with Biodentine or mineral trioxide aggregate (MTA). MATERIALS AND METHODS: The pulp chamber floor of right upper first molars of 60 rats was perforated and filled with Biodentine, MTA, or cotton pellet (sham); the left first molars were used as control. After 7, 15, 30, and 60 days, maxillary fragments were processed for paraffin-embedding. The periodontal space (PS), volume density of inflammatory cells (VvIC) and fibroblasts (VvFb), number of osteoclasts, and collagen content were obtained. Interleukin-6 (IL-6) and osterix (osteoblast marker) were detected by immunohistochemistry. The data were submitted to ANOVA and Tukey's test (p ≤ 0.05). RESULTS: At 7 days, high values in VvIC, IL-6-immunolabeled cells, and osteoclasts were accompanied by reduced collagen content in enlarged PS of experimental groups. At all periods, VvIC, number of osteoclasts and IL-6, and PS were higher in sham than in Biodentine and MTA (p < 0.0001). From 7 to 60 days, significant reduction in VvIC, IL-6 immunoexpression, and osteoclasts was accompanied by significant increase in VvFb, osteoblasts, and collagen in Biodentine and MTA groups. At 60 days, significant differences in VvIC, PS, IL-6, osteoclasts, and osteoblasts were not found between Biodentine and MTA. Significant differences in the osteoclast number were not observed among Biodentine, MTA, and control groups while osteoblasts number was higher in Biodentine and MTA groups. CONCLUSIONS: Despite the initial inflammatory reaction and bone resorption, the sealing of furcation perforations with Biodentine and MTA favors the repair of periodontal tissues. CLINICAL RELEVANCE: Biodentine and MTA exhibit potential as repair material in the treatment of furcation perforations.


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Dente Molar/patologia , Osteogênese , Óxidos/farmacologia , Silicatos/farmacologia , Animais , Combinação de Medicamentos , Masculino , Pemetrexede , Ratos
17.
Braz Oral Res ; 32(suppl 1): e68, 2018 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-30365609

RESUMO

Endodontic medicine, which addresses the bidirectional relationship between endodontic infections and systemic diseases, has gained prominence in the field of endodontics. There is much evidence showing that while systemic disease may influence the pathogenesis of endodontic infection, endodontic infection can also cause systemic alterations. These alterations include more severe bone resorption and inflammation in the periapical area as well as enhanced systemic disease symptoms. Similarly, many reports have described the impact of systemic diseases on the tissue responses to dental materials. Conversely, the local use of dental materials may show systemic effects in the form of altered production of biomarkers. Thus, studies to better understand the mechanisms related to those connections are extremely important. In this context, the objective of this review was to analyze and discuss the current literature regarding the connections among these three factors-systemic diseases, endodontic infection, and endodontic dental materials-and determine how these connections may interfere in the systemic health status and the endodontic treatment outcomes, which are represented by periapical wound healing.


Assuntos
Doenças Cardiovasculares/fisiopatologia , Polpa Dentária/efeitos dos fármacos , Diabetes Mellitus/fisiopatologia , Periodontite Periapical/fisiopatologia , Materiais Restauradores do Canal Radicular/farmacologia , Tela Subcutânea/efeitos dos fármacos , Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Doenças da Polpa Dentária/fisiopatologia , Combinação de Medicamentos , Humanos , Doenças Metabólicas/fisiopatologia , Óxidos/farmacologia , Fatores de Risco , Silicatos/farmacologia
18.
Braz Oral Res ; 32: e103, 2018 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-30328900

RESUMO

The aim of this study is to evaluate the expression of cytokines in response to mineral trioxide aggregate (MTA) plus selenium in germ-free mice with experimental furcal perforation. The first left maxillary molar was opened, and the furcal area was perforated and treated with post-MTA-Se (experimental group). The same surgical intervention was performed for the maxillary right first molar, which was treated with MTA (control group). Fifteen mice were sacrificed 7, 14, and 21 days after furcal perforation, and periapical tissue samples were collected. The mRNA expression levels of the cytokines TGF-ß, TNF-α, IFN-γ, HPRT, IL-10, IL-4, RANK, RANKL, IL-1, and IL-17 were assessed by using real-time polymerase chain reaction. In the experimental group, at 21-days post-MTA-Se sealing, the mRNA levels of TNF-α and IL-10 were upregulated compared with those in the control group (p < 0.05). Futher assessment revealed basal mRNA expression levels of IL-1α, IFN-γ, RANK, RANKL, IL-17A, IL-4, and TGF-ß, over long experimental times, in both the experimental and control groups (p > 0.05). In conclusion, MTA+Se sealing favoured increased expression of IL-10 and TNF-α at later time points (day 21).


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Citocinas/análise , Cavidade Pulpar/lesões , Defeitos da Furca/tratamento farmacológico , Óxidos/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Selênio/farmacologia , Silicatos/farmacologia , Animais , Cavidade Pulpar/efeitos dos fármacos , Cavidade Pulpar/imunologia , Combinação de Medicamentos , Feminino , Defeitos da Furca/imunologia , Masculino , Camundongos , Dente Molar/efeitos dos fármacos , Dente Molar/lesões , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes , Tratamento do Canal Radicular/métodos , Fatores de Tempo , Resultado do Tratamento
19.
J Endod ; 44(10): 1542-1548, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30170844

RESUMO

INTRODUCTION: In this study, we examined the effect of mineral trioxide aggregate (MTA) on macrophage polarization and the potential involvement of Axl/nuclear factor kappa B (NF-κB) signaling in mediating the effect of MTA. METHODS: The human monocyte cell line THP-1 was cultured with MTA solution for 1, 2, or 3 days, and the population change of M2 macrophages was analyzed by flow cytometry. Expression of M2 cytokines was examined by enzyme-linked immunosorbent assay. Phagocytosis and angiogenesis-induction ability were also assayed. The involvement of Axl/NF-κB signaling in MTA-treated cells was examined by analyzing phosphorylation status of Axl, Akt, IKKα/ß, and IκBα. Specific inhibitors for Axl/Akt/NF-κB signaling were added to MTA-treated THP-1 cells, and their cytokine expression change was examined. RESULTS: Flow cytometry analysis showed that MTA treatment increased CD206+ cells in a time-dependent way. After MTA treatment, the expression of M2-related cytokines was up-regulated. MTA also enhanced phagocytic ability and the ability of THP-1 cells to induce angiogenesis. Treatment of MTA led to activate Axl/Akt/NF-kB signal axis by phosphorylation of Axl, Akt, IKKα/ß, IκBα, and p65. In addition, MTA-induced interleukin 10, transforming growth factor beta, and vascular endothelial growth factor expression was suppressed as specific inhibitors were added. CONCLUSIONS: Our findings indicate that MTA is able to induce macrophage polarization toward the M2 phenotype, with up-regulation of interleukin 10, transforming growth factor beta, and vascular endothelial growth factor, and that Axl/Akt/NF-κB signaling participates in this process. These results provide the cellular and molecular basis of MTA's anti-inflammatory action in clinical applications.


Assuntos
Compostos de Alumínio/farmacologia , Anti-Inflamatórios , Compostos de Cálcio/farmacologia , Polaridade Celular/efeitos dos fármacos , Polaridade Celular/genética , Macrófagos/fisiologia , NF-kappa B/metabolismo , NF-kappa B/fisiologia , Óxidos/farmacologia , Proteínas Proto-Oncogênicas/metabolismo , Proteínas Proto-Oncogênicas/fisiologia , Receptores Proteína Tirosina Quinases/metabolismo , Receptores Proteína Tirosina Quinases/fisiologia , Transdução de Sinais/fisiologia , Silicatos/farmacologia , Células Cultivadas , Citocinas/metabolismo , Combinação de Medicamentos , Humanos , Macrófagos/imunologia , Neovascularização Fisiológica/genética , Neovascularização Fisiológica/fisiologia , Fagocitose , Fosforilação/efeitos dos fármacos , Fosforilação/genética , Células THP-1 , Regulação para Cima/efeitos dos fármacos , Regulação para Cima/genética
20.
Eur J Oral Sci ; 126(4): 326-333, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29961994

RESUMO

A new mineral trioxide aggregate (MTA) material has been developed with a modified composition that requires investigations to support its clinical use. This study evaluated the biocompatibility and biomineralization of this new MTA material and compared it with that of two other MTA cements over time. Tubes containing materials (or empty tubes as controls) were inserted into the subcutaneous tissues of 40 rats. On days 7, 15, 30, 60, and 90, the tubes were removed with the surrounding tissues, which were either stained with haematoxylin and eosin or von Kossa for further analyses or unstained for observation under polarized light. On days 7 and 15, moderate inflammation was observed in most specimens, and the fibrous capsule was thick. On day 30, there was mild inflammation in all groups, and the fibrous capsule was thin. On days 60 and 90, there was mild inflammation in the material groups, while the control group showed no inflammation, although no statistically significant difference between the groups was observed and the fibrous capsule was thin. All material groups showed structures that stained with von Kossa and could be observed under polarized light; this was not found for the control. In conclusion, the new MTA material had biocompatibility and biomineralization properties similar to those of the two existing MTA materials.


Assuntos
Compostos de Alumínio/farmacologia , Biomineralização/efeitos dos fármacos , Compostos de Cálcio/farmacologia , Cimentos Dentários/farmacologia , Óxidos/farmacologia , Silicatos/farmacologia , Tela Subcutânea/efeitos dos fármacos , Animais , Materiais Biocompatíveis/farmacologia , Bismuto , Combinação de Medicamentos , Implantes de Medicamento , Inflamação , Masculino , Ratos , Ratos Wistar
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