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1.
Water Sci Technol ; 82(6): 1142-1154, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33055404

RESUMO

Microbial consortia are effective biofilters to treat wastewaters, allowing for resource recovery and water remediation. To reuse and save water in the domestic cycle, we assembled a suspended biofilm, a 'biofilter' to treat dishwasher wastewater. Bacterial monocultures of both photo- and heterotrophs were assembled in an increasingly complex fashion to test their nutrient stripping capacity. This 'biofilter' is the core of an integrated system (Zero Mile System) devoted to reusing and upcycling of reconditioned wastewater, partly in subsequent dishwasher cycles and partly into a vertical garden for plant food cultivation. The biofilter was assembled based on a strain of the photosynthetic, filamentous cyanobacterium Trichormus variabilis, selected to produce an oxygen evolving scaffold, and three heterotrophic aerobic bacterial isolates coming from the dishwasher wastewater itself: Acinetobacter, Exiguobacterium and Pseudomonas spp. The consortium was constructed starting with 16 isolates tested one-to-one with T. variabilis and then selecting the heterotrophic microbes up to a final one-to-three consortium, which included two dominant and a rare component of the wastewater community. This consortium thrives in the wastewater much better than T. variabilis alone, efficiently stripping N and P in short time, a pivotal step for the reuse and saving of water in household appliances.


Assuntos
Águas Residuárias , Purificação da Água , Bactérias/genética , Consórcios Microbianos , Nutrientes
2.
Mar Pollut Bull ; 159: 111489, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32892922

RESUMO

A bacterial consortium immobilized in magnetic floating biochar gel beads is proposed to remove high-molecular-weight polycyclic aromatic hydrocarbons. The microbial consortium performed better than single strains and consisted of four strains of marine bacteria for degrading pyrene (PYR), two strains for benzo(a)pyrene (BAP), and three strains for indeno(1,2,3-cd)pyrene (INP), which were isolated from oil-contaminated seawater. The immobilized cells could biodegrade 89.8%, 66.9% and 78.2% of PYR, BAP and INP, respectively, and had better tolerance to pH, temperature and salinity than free cells. The Andrews model was used to explore the biodegradation kinetics, and when the initial concentrations of PYR, BAP, and INP were 7.80, 3.05, and 3.41 mg/L, the specific biodegradation rates reached maximum values of 0.2507, 0.1286, and 0.1930 d-1, respectively. The immobilized microbial consortium had a high HMW-PAH removal ability and good floatability and magnetic properties and could be collected by an external magnetic field.


Assuntos
Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , Biodegradação Ambiental , Carvão Vegetal , Campos Magnéticos , Fenômenos Magnéticos , Consórcios Microbianos
3.
Nat Commun ; 11(1): 4018, 2020 08 11.
Artigo em Inglês | MEDLINE | ID: mdl-32782301

RESUMO

The gut microbiome is an ecosystem that involves complex interactions. Currently, our knowledge about the role of the gut microbiome in health and disease relies mainly on differential microbial abundance, and little is known about the role of microbial interactions in the context of human disease. Here, we construct and compare microbial co-abundance networks using 2,379 metagenomes from four human cohorts: an inflammatory bowel disease (IBD) cohort, an obese cohort and two population-based cohorts. We find that the strengths of 38.6% of species co-abundances and 64.3% of pathway co-abundances vary significantly between cohorts, with 113 species and 1,050 pathway co-abundances showing IBD-specific effects and 281 pathway co-abundances showing obesity-specific effects. We can also replicate these IBD microbial co-abundances in longitudinal data from the IBD cohort of the integrative human microbiome (iHMP-IBD) project. Our study identifies several key species and pathways in IBD and obesity and provides evidence that altered microbial abundances in disease can influence their co-abundance relationship, which expands our current knowledge regarding microbial dysbiosis in disease.


Assuntos
Microbioma Gastrointestinal , Doenças Inflamatórias Intestinais/microbiologia , Consórcios Microbianos , Obesidade/microbiologia , Adulto , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , Bactérias/metabolismo , Estudos de Coortes , Disbiose/metabolismo , Disbiose/microbiologia , Feminino , Microbioma Gastrointestinal/genética , Especificidade de Hospedeiro , Humanos , Doenças Inflamatórias Intestinais/metabolismo , Masculino , Redes e Vias Metabólicas , Pessoa de Meia-Idade , Obesidade/metabolismo
4.
Science ; 369(6507)2020 08 28.
Artigo em Inglês | MEDLINE | ID: mdl-32855308

RESUMO

Microbial consortia are a promising alternative to monocultures of genetically modified microorganisms for complex biotransformations. We developed a versatile consortium-based strategy for the direct conversion of lignocellulose to short-chain fatty acids, which included the funneling of the lignocellulosic carbohydrates to lactate as a central intermediate in engineered food chains. A spatial niche enabled in situ cellulolytic enzyme production by an aerobic fungus next to facultative anaerobic lactic acid bacteria and the product-forming anaerobes. Clostridium tyrobutyricum, Veillonella criceti, or Megasphaera elsdenii were integrated into the lactate platform to produce 196 kilograms of butyric acid per metric ton of beechwood. The lactate platform demonstrates the benefits of mixed cultures, such as their modularity and their ability to convert complex substrates into valuable biochemicals.


Assuntos
Clostridium tyrobutyricum/metabolismo , Ácidos Graxos Voláteis/biossíntese , Lignina/metabolismo , Megasphaera elsdenii/metabolismo , Consórcios Microbianos , Veillonella/metabolismo , Biotransformação , Ácido Láctico/metabolismo
5.
Nat Commun ; 11(1): 4236, 2020 08 25.
Artigo em Inglês | MEDLINE | ID: mdl-32843654

RESUMO

The impact of commensal bacteria on the host arises from complex microbial-diet-host interactions. Mapping metabolic interactions in gut microbial communities is therefore key to understand how the microbiome influences the host. Here we use an interdisciplinary approach including isotope-resolved metabolomics to show that in Drosophila melanogaster, Acetobacter pomorum (Ap) and Lactobacillus plantarum (Lp) a syntrophic relationship is established to overcome detrimental host diets and identify Ap as the bacterium altering the host's feeding decisions. Specifically, we show that Ap uses the lactate produced by Lp to supply amino acids that are essential to Lp, allowing it to grow in imbalanced diets. Lactate is also necessary and sufficient for Ap to alter the fly's protein appetite. Our data show that gut bacterial communities use metabolic interactions to become resilient to detrimental host diets. These interactions also ensure the constant flow of metabolites used by the microbiome to alter reproduction and host behaviour.


Assuntos
Dieta , Drosophila melanogaster/microbiologia , Drosophila melanogaster/fisiologia , Microbioma Gastrointestinal/fisiologia , Acetobacter/crescimento & desenvolvimento , Acetobacter/metabolismo , Aminoácidos/deficiência , Aminoácidos/metabolismo , Animais , Apetite , Feminino , Preferências Alimentares , Interações entre Hospedeiro e Microrganismos , Ácido Láctico/metabolismo , Lactobacillus plantarum/crescimento & desenvolvimento , Lactobacillus plantarum/metabolismo , Redes e Vias Metabólicas , Metabolômica , Consórcios Microbianos , Reprodução
6.
Artigo em Inglês | MEDLINE | ID: mdl-32791105

RESUMO

In this issue of Cell Host & Microbe, Adebayo et al. (2020) examine the urobiome of older community-dwelling women within the TwinUK Cohort. They define a core genitourinary microbiome for older women with many heritable microbial taxa. Some taxa appear to co-occur, suggesting the existence of specific microbial consortia.


Assuntos
Microbiota , Sistema Urinário , Idoso , Estudos de Coortes , Feminino , Humanos , Vida Independente , Consórcios Microbianos
7.
Bioresour Technol ; 314: 123739, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32615449

RESUMO

Syngas biomethanation is an attractive process for extending application of gasification products. In the present study, anaerobic sludges from three methanogenic reactors feeding cattle manure (CS), sewage sludge (SS) and gaseous H2/CO2 (GS) were used to investigate the effect of microbial consortia composition on syngas biomethanation. The results showed that CS presented the highest CO consumption rate due to its highest relative abundance of CO consuming bacteria. The CO was mainly converted to acetate, and syntrophic acetate oxidization (SAO) bacteria converted acetate to H2/CO2 for hydrogenotrophic methanogenesis in CS and SS. However, acetate was accumulated in GS for lacking acetoclastic methanogens and SAO bacteria, leading to lower biomethanation efficiency. Additionally, adding stoichiometric H2 could convert CO and CO2 to nearly pure methane, while, the CO consumption rate declined in H2 added systems. The results present novel insights into microbial consortia on CO conversion and syngas biomethanation.


Assuntos
Monóxido de Carbono , Consórcios Microbianos , Anaerobiose , Animais , Reatores Biológicos , Bovinos , Metano , Esgotos
8.
Bioresour Technol ; 314: 123741, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32650263

RESUMO

A lab-scale three dimensional biofilm-electrode reactor (3DBER) coupled with sulfur/iron (3DBER-Fe/S) system was established to examine the impacts of current gradient on the performances and microbial network dynamics. Results showed that generally low current could promote nitrogen and phosphorus removal, while high current caused the inhibition of nutrients removal. Molecular ecological network (MEN) analysis showed that the current altered the overall architecture of the networks, and low currents could improve the scale and complexity of networks (<100 mA), while high current (≥100 mA) likely decrease the networks scale and complexity. Stronger competition was observed among Proteobacteria and Chloroflexi at high current conditions, which may be relevant to the deterioration of nutrients removal. In addition, the current dramatically altered the network interactions among denitrifiers, and the keystone species were intensively dynamic among various networks under the current gradient.


Assuntos
Reatores Biológicos , Desnitrificação , Consórcios Microbianos , Nitrogênio , Fósforo
9.
Nat Commun ; 11(1): 3659, 2020 07 21.
Artigo em Inglês | MEDLINE | ID: mdl-32694598

RESUMO

As synthetic biocircuits become more complex, distributing computations within multi-strain microbial consortia becomes increasingly beneficial. However, designing distributed circuits that respond predictably to variation in consortium composition remains a challenge. Here we develop a two-strain gene circuit that senses and responds to which strain is in the majority. This involves a co-repressive system in which each strain produces a signaling molecule that signals the other strain to down-regulate production of its own, orthogonal signaling molecule. This co-repressive consortium links gene expression to ratio of the strains rather than population size. Further, we control the cross-over point for majority via external induction. We elucidate the mechanisms driving these dynamics by developing a mathematical model that captures consortia response as strain fractions and external induction are varied. These results show that simple gene circuits can be used within multicellular synthetic systems to sense and respond to the state of the population.


Assuntos
Engenharia Celular/métodos , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Consórcios Microbianos/genética , Percepção de Quorum/genética , Redes Reguladoras de Genes , Transdução de Sinais/genética , Biologia Sintética/métodos
10.
Sci Total Environ ; 729: 139020, 2020 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-32498175

RESUMO

Increased global warming, caused by climate change and human activities, will seriously hinder plant development, such as increasing salt concentrations in soils, which will limit water availability for plants. To ensure optimal plant growth under such changing conditions, microorganisms that improve plant growth and health must be integrated into agricultural practices. In the present work, we examined the fate of Vicia faba microbiota structure and interaction network upon inoculation with plant-nodulating rhizobia (Rhizobium leguminosarum RhOF125) and non-nodulating strains (Paenibacillus mucilaginosus BLA7 and Ensifer meliloti RhOL1) in the presence (or absence) of saline stress. Inoculated strains significantly improved plant tolerance to saline stress, suggesting either a direct or indirect effect on the plant response to such stress. To determine the structure of microbiota associated with V. faba, samples of the root-adhering soil (RAS), and the root tissues (RT) of seedlings inoculated (or not) with equal population size of RhOF125, BLA7 and RhOL1 strains and grown in the presence (or absence) of salt, were used to profile the microbial composition by 16S rRNA gene sequencing. The inoculation did not show a significant impact on the composition of the RT microbiota or RAS microbiota. The saline stress shifted the RAS microbiota composition, which correlated with a decrease in Enterobacteriaceae and an increase in Sphingobacterium, Chryseobacterium, Stenotrophomonas, Agrobacterium and Sinorhizobium. When the microbiota of roots and RAS are considered together, the interaction networks for each treatment are quite different and display different key populations involved in community assembly. These findings indicate that upon seed inoculation, community interaction networks rather than their composition may contribute to helping plants to better tolerate environmental stresses. The way microbial populations interfere with each other can have an impact on their functions and thus on their ability to express the genes required to help plants tolerate stresses.


Assuntos
Vicia faba , Bactérias , Humanos , Consórcios Microbianos , Interações Microbianas , Raízes de Plantas , RNA Ribossômico 16S , Microbiologia do Solo
11.
Chemosphere ; 258: 127289, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32535447

RESUMO

Anammox-based processes and microbial consortia have drawn extensive attention for their use in high-efficiency wastewater treatment technologies. Metals substantially affect the activity of anammox consortia and the quality of wastewater treatment plant effluent. Here, we explored the role of anammox consortia in terms of metals complexation in both single and multi-metal systems. Adsorption edges of single metal cations indicate that the adsorption preference was in the order: Pb(II) > Cd(II) > Cr(VI). A competitive effect was observed in multi-metal cations systems, with Pb(II) being preferably adsorbed and the degree of adsorption somewhat reduced in the presence of either Cd(II) or Cr(VI), while Cd(II) and Cr(VI) were easily exchanged and substituted by other metals. Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) further suggest that the adsorption of Pb(II) and Cd(II) are as inner-sphere ion-exchange mechanisms, while Cr(VI) adsorption is mainly by outer-sphere complexation. Density functional theory (DFT) calculations highlight that Cd(II) and Pb(II) have different binding sites compared to Cr(VI), and the order of binding energy (Ebd) of three metal cations were Pb(II) > Cd(II) > Cr(VI). These calculations support the adsorption data in that Pb forms more stable complexes with anammox bacterial surface ligands. Surface complexation modelling (SCM) further predicted both the sorption of single metal cations and competitive adsorption of the three metals to anammox consortia, the exception being Cd at higher loadings. The results of this study highlight the potential role of anammox consortia in removing metal cations from wastewater in treatment systems.


Assuntos
Amônia/metabolismo , Reatores Biológicos/microbiologia , Metais Pesados/química , Consórcios Microbianos/fisiologia , Adsorção , Anaerobiose , Cátions , Teoria da Densidade Funcional , Metais Pesados/metabolismo , Oxirredução , Espectroscopia Fotoeletrônica , Espectroscopia de Infravermelho com Transformada de Fourier , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/metabolismo
12.
Mar Pollut Bull ; 156: 111244, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32510386

RESUMO

In order to determine the practicability of developing a protocol for bioassessing polar marine environment based on network analysis, microplankton communities and co-occurrence patterns at Ardley Cove and Great Wall Cove (King George Island, Antarctica) were studied in January 2016 through high-through sequencing. The spatial patterns and significant differences between community structures in two coves clearly reflect those in environmental heterogeneity. Moreover, both coves had their discriminated network structure and keystones. Then multivariate analyses to quantify the relationship between environmental variation and planktonic microbes response, give further evidence that nitrate and temperature, alone or in combination with other several parameters, structuring the communities respectively indeed. This study presents the first detailed description on co-occurrence networks between microbes and local environmental parameters in Antarctic coastal water. These findings suggest that co-occurrence networks based on planktonic microbes have the robust potential to assess environmental heterogeneity in polar marine ecosystem.


Assuntos
Ecossistema , Consórcios Microbianos , Regiões Antárticas , Ilhas , Plâncton
13.
Bioresour Technol ; 311: 123516, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32428849

RESUMO

Crude glycerol is by-product produced from biodiesel industry and can be converted directly by mixed microbial culture (MMC) into polyhydroxyalkanoates (PHAs). This study investigated the effects of the reverse (SBR_A) and positive (SBR_B) glycerol gradient substrate strategy on PHA-accumulating culture enrichment and the maximum PHA accumulating stability under substrates with different glycerol and volatile fatty acid (VFA) proportion. The results showed that crude glycerol was mainly used for PHA production rather than biomass growth in SBR_A. The maximum qPHA was 0.65 g COD/g X-1·h-1 under sole crude glycerol condition in SBR_A, which was 2.41 times higher than that of SBR_B. Moreover, the PHA accumulating ability of the biomass from SBR_A was more stable than SBR_B. Saccharibacteria_genera_incertae_sedis was for the first time found to be the dominant genus using crude glycerol for PHA production. This research provides an insight into enrichment strategy to effectively enrich PHA-accumulating culture from crude glycerol.


Assuntos
Poli-Hidroxialcanoatos , Reatores Biológicos , Ácidos Graxos Voláteis , Glicerol , Consórcios Microbianos
14.
Nature ; 581(7809): 475-479, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32461639

RESUMO

Intestinal health relies on the immunosuppressive activity of CD4+ regulatory T (Treg) cells1. Expression of the transcription factor Foxp3 defines this lineage, and can be induced extrathymically by dietary or commensal-derived antigens in a process assisted by a Foxp3 enhancer known as conserved non-coding sequence 1 (CNS1)2-4. Products of microbial fermentation including butyrate facilitate the generation of peripherally induced Treg (pTreg) cells5-7, indicating that metabolites shape the composition of the colonic immune cell population. In addition to dietary components, bacteria modify host-derived molecules, generating a number of biologically active substances. This is epitomized by the bacterial transformation of bile acids, which creates a complex pool of steroids8 with a range of physiological functions9. Here we screened the major species of deconjugated bile acids for their ability to potentiate the differentiation of pTreg cells. We found that the secondary bile acid 3ß-hydroxydeoxycholic acid (isoDCA) increased Foxp3 induction by acting on dendritic cells (DCs) to diminish their immunostimulatory properties. Ablating one receptor, the farnesoid X receptor, in DCs enhanced the generation of Treg cells and imposed a transcriptional profile similar to that induced by isoDCA, suggesting an interaction between this bile acid and nuclear receptor. To investigate isoDCA in vivo, we took a synthetic biology approach and designed minimal microbial consortia containing engineered Bacteroides strains. IsoDCA-producing consortia increased the number of colonic RORγt-expressing Treg cells in a CNS1-dependent manner, suggesting enhanced extrathymic differentiation.


Assuntos
Bactérias/metabolismo , Ácidos e Sais Biliares/química , Ácidos e Sais Biliares/metabolismo , Linfócitos T Reguladores/citologia , Linfócitos T Reguladores/imunologia , Sequência de Aminoácidos , Animais , Bacteroides/metabolismo , Colo/microbiologia , Células Dendríticas/imunologia , Células Dendríticas/metabolismo , Feminino , Fermentação , Microbioma Gastrointestinal , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Consórcios Microbianos , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/metabolismo , Receptores Citoplasmáticos e Nucleares/metabolismo
15.
Ecotoxicol Environ Saf ; 200: 110734, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32464440

RESUMO

Microorganisms' role in pesticide degradation has been studied widely. Insitu treatments of effluents containing pesticides such as biological beds (biobeds) are efficient biological systems where biomixture (mixture of substrates) and microorganisms are the keys in pesticide treatment; however, microbial activity has been studied poorly, and its potential beyond biobeds has not been widely explored. In this study, the capacity of microbial consortium and bacteria-pure strains isolated from a biomixture (soil-straw; 1:1, v/v) used to treat agricultural effluents under real conditions were evaluated during a bioremediation process of five pesticides commonly used Yucatan Mexico. Atrazine, carbofuran, and glyphosate had the highest degradations (>90%) using the microbial consortium; 2,4-D and diazinon were the most persistent (DT50 = 8.64 and 6.63 days). From the 21 identified bacteria species in the microbial consortium, Pseudomonas nitroreducens was the most abundant (52%) according to identified sequences. For the pure strains evaluation 2,4-D (DT50 = 9.87 days), carbofuran (DT50 = 8.27 days), diazinon (DT50 = 8.80 days) and glyphosate (DT50 = 8.59 days) were less persistent in the presence of the mixed consortium (Ochrobactrum sp. DGG-1-3, Ochrobactrum sp. Ge-14, Ochrobactrum sp. B18 and Pseudomonas citronellolis strain ADA-23B). Time, pesticide, and strain type were significant (P < 0.05) in pesticide degradation, so this process is multifactorial. Microbial consortium and pure strains can be used to increase the biobed efficiency by inoculation, even in the remediation of soil contaminated by pesticides in agricultural areas.


Assuntos
Bactérias/metabolismo , Consórcios Microbianos , Praguicidas/metabolismo , Poluentes do Solo/metabolismo , Ácido 2,4-Diclorofenoxiacético/metabolismo , Agricultura , Atrazina/metabolismo , Bactérias/isolamento & purificação , Biodegradação Ambiental , Carbofurano/metabolismo , Diazinon/metabolismo , Glicina/análogos & derivados , Glicina/metabolismo , Pseudomonas/isolamento & purificação , Solo/química
16.
Biodegradation ; 31(3): 171-182, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32361902

RESUMO

Cometabolic biodegradation of 1,4-dioxane (dioxane) in the presence of primary substrates is a promising strategy for treating dioxane at environmentally relevant concentrations. Seven aqueous amendments (i.e., tetrahydrofuran (THF), butanone, acetone, 1-butanol, 2-butanol, phenol and acetate) and five gaseous amendments (i.e., C1-C4 alkanes and ethylene) were evaluated as the primary substrates for dioxane degradation by mixed microbial consortia. The aqueous amendments were tested in microcosm bottles and the gaseous amendments were tested in a continuous-flow membrane biofilm reactor with hollow fibers pressurized by the gaseous amendments. Ethane was found to be the most effective gaseous substrate and THF was the only aqueous substrate that promoted dioxane degradation. A diverse microbial community consisting of several putative dioxane degraders-Mycobacterium, Flavobacterium and Bradyrhizobiaceae-were enriched in the presence of ethane. This is the first study showing that ethane was the most effective substrate among the short-chain alkanes and it promoted dioxane degradation by enriching dioxane-degraders that did not harbor the well-known dioxane/tetrahydrofuran monooxygenase.


Assuntos
Furanos , Consórcios Microbianos , Biodegradação Ambiental , Dioxanos , Etano
17.
PLoS One ; 15(5): e0229889, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32396555

RESUMO

The purpose of the study involves the development of an anaerobic, thermophilic microbial consortium TERIK from the high temperature reservoir of Gujarat for enhance oil recovery. To isolate indigenous microbial consortia, anaerobic baltch media were prepared and inoculated with the formation water; incubated at 65°C for 10 days. Further, the microbial metabolites were analyzed by gas chromatography, FTIR and surface tension. The efficiency of isolated consortia towards enhancing oil recovery was analyzed through core flood assay. The novelty of studied consortia was that, it produces biomass (600 mg/l), bio-surfactant (325 mg/l), and volatile fatty acids (250 mg/l) at 65°C in the span of 10 days, that are adequate to alter the surface tension (70 to 34 mNm -1) and sweep efficiency of zones facilitating the displacement of oil. TERIK was identified as Clostridium sp. The FTIR spectra of biosurfactant indicate the presence of N-H stretch, amides and polysaccharide. A core flooding assay was designed to explore the potential of TERIK towards enhancing oil recovery. The results showed an effective reduction in permeability at residual oil saturation from 2.14 ± 0.1 to 1.39 ± 0.05 mD and 19% incremental oil recovery.


Assuntos
Archaea/metabolismo , Microbiologia Industrial , Consórcios Microbianos , Campos de Petróleo e Gás/microbiologia , Clostridium/metabolismo , Temperatura Alta , Humanos , Petróleo/microbiologia , Tensão Superficial , Tensoativos/farmacologia
18.
Nat Commun ; 11(1): 2418, 2020 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-32415107

RESUMO

The spatial organization of microbial communities arises from a complex interplay of biotic and abiotic interactions, and is a major determinant of ecosystem functions. Here we design a microfluidic platform to investigate how the spatial arrangement of microbes impacts gene expression and growth. We elucidate key biochemical parameters that dictate the mapping between spatial positioning and gene expression patterns. We show that distance can establish a low-pass filter to periodic inputs and can enhance the fidelity of information processing. Positive and negative feedback can play disparate roles in the synchronization and robustness of a genetic oscillator distributed between two strains to spatial separation. Quantification of growth and metabolite release in an amino-acid auxotroph community demonstrates that the interaction network and stability of the community are highly sensitive to temporal perturbations and spatial arrangements. In sum, our microfluidic platform can quantify spatiotemporal parameters influencing diffusion-mediated interactions in microbial consortia.


Assuntos
Dispositivos Lab-On-A-Chip , Consórcios Microbianos , Transdução de Sinais , Ecologia , Ecossistema , Desenho de Equipamento , Escherichia coli/fisiologia , Microbioma Gastrointestinal , Regulação Bacteriana da Expressão Gênica , Microfluídica/instrumentação , Modelos Genéticos , Oscilometria , Percepção de Quorum
19.
Bioresour Technol ; 309: 123331, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32283484

RESUMO

Biohydrogen production was evaluated using cassava processing wastewater (CPW) and two microbial consortia (Vir and Gal) from different Brazilian environments. The biohydrogen production was optimized using a Box-Behnken design (T, pH, C/N, and % v/v inoculum). Maximum yields were obtained with hydrolyzed substrate: 4.12 and 3.80 mol H2 / for Vir and Gal, respectively. Similarly, the kinetic parameters µ, k, and q were higher with hydrolyzed CPW in both consortia. The molecular analysis of the consortia through Illumina high-throughput sequencing showed the presence of bacteria from the families Porphyromonadaceae, Clostridiaceae, Ruminococcaceae, and Enterococcaceae. The relative abundance of microbial families varies as fermentation progresses. In both consortia, Clostridiaceae reached the maximum relative abundance in the media between 16 and 24 h, interval in which approximately 90% of the biohydrogen is generated.


Assuntos
Manihot , Águas Residuárias , Reatores Biológicos , Brasil , Fermentação , Hidrogênio , Cinética , Consórcios Microbianos
20.
Bioresour Technol ; 309: 123313, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32289659

RESUMO

The performance and behavior of microbial fuel cells (MFCs) are influenced by among others the external load (Rext). In this study, the anode-surface biofilm formation in MFCs operated under different Rext selection/tracking-strategies was assessed. MFCs were characterized by electrochemical (voltage/current generation, polarization tests, EIS), molecular biological (microbial consortium analysis) and bioinformatics (principal component analysis) tools. The results indicated that the MFC with dynamic Rext adjustment (as a function of the actual MFC internal resistance) achieved notably higher performance but relatively lower operational stability, mainly due to the acidification of the biofilm. The opposite (lower performance, increased stability) could be observed with the static (low or high) Rext application (or OCV) strategies, where adaptive microbial processes were assumed. These possible adaptation phenomena were outlined by a theoretical framework and the significant impact of Rext on the anode colonization process and energy recovery with MFCs was concluded.


Assuntos
Fontes de Energia Bioelétrica , Biofilmes , Eletricidade , Eletrodos , Consórcios Microbianos
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