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1.
Forensic Sci Int ; 305: 109998, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31707237

RESUMO

Bruising is an injury commonly observed within suspect cases of assault or abuse, yet how a blunt impact initiates bruising and influences its severity is not fully understood. Furthermore, the standard method of documenting a bruise with colour photography is known to have limitations which influence the already subjective analysis of a bruise. This research investigated bruising using a standardised blunt impact, delivered to 18 volunteers. The resulting bruise was imaged using colour, cross polarised (CP) and infrared photography. Timelines of the L*a*b* colour space were determined from both colour and CP images for up to 3 weeks. Overall, no single photographic technique out-performed the others, however CP did provide greater contrast than colour photography. L*a*b* colour space timelines were not attributable any physiological characteristics. Whilst impact force negatively correlated with BMI (R2 = 0.321), neither were associated with any measure of bruise appearance. Due to the inter-subject variability in the bruise response to a controlled infliction, none of the methods in the current study could be used to reliably predict the age of a bruise or the severity of force used in creating a bruise. A more comprehensive approach combining impact characteristics, tissue mechanics, enhanced localised physiological measures and improvements in quantifying bruise appearance is likely to be essential in removing subjectivity from their interpretation.


Assuntos
Contusões/patologia , Pele/patologia , Adulto , Fenômenos Biomecânicos , Índice de Massa Corporal , Feminino , Medicina Legal/métodos , Humanos , Processamento de Imagem Assistida por Computador , Masculino , Fotografação/métodos , Pele/lesões , Fatores de Tempo , Adulto Jovem
2.
Forensic Sci Int ; 303: 109937, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31546162

RESUMO

The detection of vitality of wounds, especially when the wounds are inflicted very close to the time of death, is one of the most challenging issues in forensic pathology. This study investigated expression levels of ATF3 and BTG2 in mouse and human skin wounds. Protein levels examined by western blot showed that there was no significant change in ATF3 and BTG2 between wounded and intact skins. However, mRNA levels demonstrated higher expression of ATF3 and BTG2 in ante-mortem contused mouse skins, compared with the intact and postmortem contused skins. Increased ATF3 and BTG2 in the level of mRNA could also be detected until 96h and 48h after death, respectively. Human wounded skin samples from forensic autopsy cases were also examined. Increased ATF3 mRNA levels were detected until 48h after autopsy in 5 of 6 cases. However, no differences were observed between wounded and intact skins for BTG2. These findings suggest that the detection of mRNA levels of ATF3, but not BTG2, can be considered as a potential marker for vital reaction of skin contusion. Postmortem human samples should be used in order to validate the availability of markers screened by animal experiment.


Assuntos
Fator 3 Ativador da Transcrição/genética , Contusões/metabolismo , RNA Mensageiro/metabolismo , Pele/metabolismo , Fator 3 Ativador da Transcrição/metabolismo , Animais , Biomarcadores/metabolismo , Genética Forense , Patologia Legal , Humanos , Proteínas Imediatamente Precoces/genética , Proteínas Imediatamente Precoces/metabolismo , Camundongos Endogâmicos BALB C , Modelos Animais , Pele/lesões , Proteínas Supressoras de Tumor/genética , Proteínas Supressoras de Tumor/metabolismo
3.
J Craniofac Surg ; 30(7): 2239-2244, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31503116

RESUMO

BACKGROUND: A skull fracture widely occurs in patients with traumatic brain injury, leading to intracranial hematoma, brain contusion, and intracranial infection. It also influences the prognosis and death of patients. This study aimed to discuss cases of patients with comminuted skull fractures. METHODS: From October 2015 to December 2018, 38 patients with comminuted skull fractures were admitted to the hospital. All patients underwent three-dimensional reconstruction of computed tomography scan images. Digital subtraction angiography or magnetic resonance venography was performed to find out the venous sinus. The clinical findings of the patients were significant regarding gender, age, injury mechanism, location, admission Glasgow Coma Scale (GCS), combined epidural, subdural, cerebral contusion, intracranial pneumatosis, maximum depth of depression, admission to surgery, dural tear, post-operative cerebrospinal fluid leakage, post-operative infection, and Glasgow Outcome Scale (GOS) 3 months after surgery. RESULTS: The incidence of traffic accidents, fall from a height, railway accidents, fall of an object, and chop injury was 60.5%, 18.4%, 13.2%, 5.3%, and 2.6%, respectively. Intra-operative dural trar negatively correlated with epidural hematoma, cerebral contusion, and subdural hematoma. Also, post-operative infection negatively correlated with intracranial pneumatosis, depth of fracture depression, and pre-operative cerebrospinal fluid leakage. No correlation was found between contusion, subdural hematoma, intracranial pneumatosis, depth of fracture depression, and post-operative infection. The GOS score positively correlated with age, pre-operative cerebrospinal fluid leakage, and admission GCS score. CONCLUSIONS: A perfect pre-operative examination is a key to successful surgery. Further studies should be conducted to find out more effective treatments for traumatic comminuted skull fractures.


Assuntos
Fratura do Crânio com Afundamento/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Vazamento de Líquido Cefalorraquidiano/etiologia , Contusões , Feminino , Escala de Coma de Glasgow , Humanos , Masculino , Pessoa de Meia-Idade , Fratura do Crânio com Afundamento/complicações , Fratura do Crânio com Afundamento/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Resultado do Tratamento , Adulto Jovem
6.
Fa Yi Xue Za Zhi ; 35(3): 267-272, 2019 Jun.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-31282618

RESUMO

Abstract: Objective To observe the change pattern of pericyte number at different time periods after mice skeletal muscle contusion and discuss its role in wound age estimation. Methods A mice gastrocnemius muscle contusion model was established. The form and number changes of pericytes at 1, 3, 5, 7, 9, 14, and 28 d post-injury were detected by multiple immunofluorescence staining. Results Compared with the slender shape of pericytes in normal skeletal muscles, pericytes in the contusion area had increased volume, rounder form and a round nuclei. Part of pericytes were found to express satellite cell markers paired-box transcription factor (Pax7) or myoblast determination 1 (MyoD1). The changes of pericyte number in skeletal muscles after contusion were time-dependant, and showed unimodal distribution with the extension of wound age. In the central contusion area, the number of pericytes peaked at 5 d post-injury while in the peripheral contusion area, the number of pericytes peaked at 5 d and 7 d post-injury. Conclusion The number of pericytes in contusion area varies time-dependently after skeletal muscle contusion in mice and might be a reference index for muscle wound age estimation, and is involved in the repair and regeneration of skeletal muscle injury.


Assuntos
Contusões , Pericitos , Animais , Modelos Animais de Doenças , Camundongos , Músculo Esquelético , Ratos Sprague-Dawley
7.
Fa Yi Xue Za Zhi ; 35(3): 280-284, 2019 Jun.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-31282620

RESUMO

Abstract: Objective To investigate the morphological changes in the degeneration and regeneration of neuromuscular junctions (NMJ) during the repair of mouse skeletal muscle contusion and discuss the correlation between the degeneration and regeneration of NMJ and wound age. Methods A total of 50 healthy adult male mice were randomly divided into 10 groups, including 9 experimental groups and 1 control group. Immunofluorescent staining was applied, and neurofilament was marked with neurofilament protein-H (NF-H), presynaptic membrane was marked with synaptophysin (Syn), presynaptic membrane was marked with acetylcholine receptor (AChR). Morphological changes of NMJ regeneration at different time points after mouse skeletal muscle contusion were detected. Results The neurofilament and presynaptic membrane of NMJ at the junction of contusion zones began to degrade after contusion, and completed degradation at about 3 d post-injury. Then they gradually regenerated, roughly completing the regeneration at about 21 d and basically reaching the control group level. The ratio of presynaptic membrane quantity to presynaptic membrane quantity showed a trend of decreasing then rising and finally reaching the control level. Conclusion During the repair of mouse skeletal muscle contusion, the morphological changes and wound age of the NMJ at the junction of contusion zones have a close correlation, which is expected to be one of the biological indicators for forensic skeletal muscle wound age estimation.


Assuntos
Contusões , Junção Neuromuscular , Animais , Masculino , Camundongos , Músculo Esquelético , Regeneração
8.
Forensic Sci Int ; 302: 109831, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31255841

RESUMO

The dating of bruises can be of crucial interest in clinical forensic medicine, but the macroscopic or photographic evaluation of a bruise has not been regarded as reliable. Several methods have thus been regularly evaluated for this purpose, with reflectance spectrometry yielding promising results. The objective of this study is to evaluate the value of a bilirubinometer as a tool for dating bruises on living victims. A clinical follow-up was carried out on patients presenting a bruise with a known onset. Using a bilirubinometer, we obtained daily measurements of the bruise (bili-bruise) and of healthy skin (bili-skin). Potential confounding factors were collected: age, sex, body mass index, trauma mechanism and Fitzpatrick skin phototype. We followed 20 patients for a total of 88 measurements of bruises. Bili-skin values showed significant differences according to skin phototype. Differences between the bili-bruise and bili-skin values (Δ-bili) followed an increase phase, peaking between 3 and 5 days, and then there was a decrease phase. No significant Δ-bili value differences were observed based on the suspected confounding factors. Our results are in favour of a peak Δ-bili value generally at day 4 or 5 post-trauma. Notably, decreasing values were not observed before day 3. Decreasing Δ-bili values would then indicate a bruise resulting from an injury formed at least 3 days before the first measurement. Complementary work confirming such data would enable improvement of the performance of bruise dating in forensic medicine.


Assuntos
Bilirrubina/metabolismo , Contusões/patologia , Patologia Legal/métodos , Pele/metabolismo , Espectrofotometria , Contusões/metabolismo , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Pigmentação da Pele , Fatores de Tempo
9.
Haemophilia ; 25(4): 558-566, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31329366

RESUMO

It has long been hypothesized that bleeding symptoms in people with hypermobility occur as a result of abnormalities in the collagen of the vessel wall or the connective tissues. The bleeding symptoms, particularly in the skin, have been attributed to the fragility of skin and blood vessels caused by "defective collagen wickerwork" of the reticular layer of the skin. Collagen, which forms the framework of vessel walls, is altered in many patients with Ehlers-Danlos syndrome (EDS) leading to weakening of the vessel wall or the supporting tissues. Another important function of subendothelial collagen is its interaction with platelets and von Willebrand factor, which results in the propagation of a platelet plug. Thus, abnormalities in subendothelial collagen may alter its interaction with platelets and VWF. More recently, hypermobile-EDS (hEDS) has been associated with mast cell disorders, a condition independently associated with bleeding symptoms. It has also been observed that patients with mild bleeding disorders have a more severe bleeding phenotype when they have co-existing joint hypermobility.


Assuntos
Síndrome de Ehlers-Danlos/classificação , Síndrome de Ehlers-Danlos/complicações , Hemorragia/etiologia , Contusões/etiologia , Síndrome de Ehlers-Danlos/metabolismo , Síndrome de Ehlers-Danlos/patologia , Humanos
10.
Zhongguo Gu Shang ; 32(6): 578-581, 2019 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-31277546

RESUMO

OBJECTIVE: To investigate the effect of Xiaozhong Zhitong Ointment(XZZTO) on remodeling and repair of skeletal muscle injury in rats based on the expression mechanism of microRNA. METHODS: The rat gastrocnemius injury model was established by blunt contusion model. The expression of MEF2 gene and protein in gastrocnemius muscle was detected by quantitative PCR at 4, 7, 14 and 21 days after injury with XZZTO. The mechanism of the effect of XZZTO on the muscle remodeling and repair of rat gastrocnemius contusion model was discussed. RESULTS: The expression level of MEF2 in the treatment group was significantly higher than that of the control group and model group, which further confirmed the important role of MEF2 in inducing skeletal muscle remodeling and repair process in the topical drugs. The expression of MEF2 increased at 7 days after injury and remained at a high level until 21 days after injury. Compared with the model group, the peak expression period was about 14 days, and then returned to the general state. CONCLUSIONS: The expression level of MEF2 shows an upward trend. Even 21 days after injury, the expression of MEF2 dose not show a significant downward trend. It can be seen that XZZTO can promote the expression of MEF2. At the same time, XZZTO can regulate the regeneration and repair of skeletal muscle. Therefore, XZZTO can play a regeneration and repair role after skeletal muscle injury through gene regulation.


Assuntos
Fatores de Transcrição MEF2/genética , Músculo Esquelético , Animais , Contusões , Trato Gastrointestinal , Regulação da Expressão Gênica , Pomadas , Proteínas , RNA Mensageiro , Ratos
11.
Zhen Ci Yan Jiu ; 44(5): 335-40, 2019 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-31155865

RESUMO

OBJECTIVE: To observe the therapeutic effect of electroacupuncture (EA) of "Zusanli" (ST36) and "Ashi"-point on the healthy side (opposing needling) on muscular injury and expression of myogenin (myoG) and fast myosin skeletal heavy chain (Fast MyHC) proteins in the gastrocnemius muscle (GM) tissues in skeletal muscle contusion rats,so as to explore its mechanism underlying improvement of skeletal muscle injury. METHODS: A total of 54 male SD rats were divided into normal control (n = 6),model (n=24) and opposing needling (EA, n=24) groups. The latter two groups were further randomized into 3, 5, 7 and 14 d subgroups (n=6 per subgroup). The skeletal muscle contusion model of the hind-limb was established by using a self-made striking device. EA (1 Hz/3 Hz,1-2 mA) was applied to ST36 and "Ashi"-point on the uninjured side of the hind-limb for 15 min every time, once a day for 3, 5, 7 and 14 days, respectively. The injured GM was harvested on the 3rd, 5th, 7th and 14th day after muscular contusion. The morphological changes of the injured GM and the mean cross-sectional areas (CSAs) of the neonatal muscle cells were observed by microscope after H.E. staining. The immunoactivity of desmin protein (myogenic marker protein of myoblast cell) of GM was detected by immunofluorescence stain on the 7th day after injury, and the expression levels of myoG (on the 3rd and 5th day after injury) and fast MyHC protein of GM tissues (on the 7thand 14th day after injury) were detected by Western blot. RESULTS: H.E. staining of GS tissue showed fewer neuronal myocytes with disordered arrangement at different sizes, and appearance of some collagenous fibers among the mesenchyme on day 7 and 14 after muscular contusion, which was relatively milder in the EA group. In the EA group, the CSA values of the neonatal muscle cells were significantly larger than those in the model group on the day 7th (P<0.05), 14th (P<0.001) after injury. On day 7 after muscular contusion, the desmin was found to express on the cellular membrane of GM in the normal control group, while in the model group, the desmin expressed mainly in the cellular plasma in the model group, and on the cellular membrane of neonatal myocytes in the EA group, respectively. The desmin positive myocytes showed disordered arrangement and different sizes after muscular contusion, whereas the situations of the EA group were close to those of the normal control group. Desmin expression was up-regulated in the EA group compared with the model group which was not significant difference (P>0.05). On the 3rd and 5th day after muscular contusion, the expression level of myoG protein was significantly up-regulated in the model group compared with the normal control group (P<0.001), and significantly up-regulated in the EA group than that in the model group (P<0.001). On the 7th and14th day after contusion, the expression level of fast MyHC protein was significantly down-regulated in the model group relevant to the normal control group (P<0.001), and markedly up-regulated in the EA group relevant to the model group (P<0.01).. CONCLUSION: EA of ST36 and "Ashi"-point on the contralateral limb can up-regulate the expression of myoG and fast MyHC proteins of GM in acute skeletal muscle contusion rats, which may contribute to its effect in promoting the repair of skeletal muscle injury.


Assuntos
Contusões , Eletroacupuntura , Pontos de Acupuntura , Animais , Extremidades , Humanos , Músculo Esquelético , Ratos , Ratos Sprague-Dawley
12.
Sports Health ; 11(6): 514-519, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31158326

RESUMO

CONTEXT: Hockey is a skillful contact sport with an elevated injury risk at higher levels of play. An understanding of injury incidence, type, mechanism, and severity at various levels of competition aids the clinician treating these athletes. The purpose of this clinical review is to discuss the epidemiology of hockey injuries at various levels of participation, including youth, high school, junior, college, and professional. EVIDENCE ACQUISITION: A literature search was performed by a review of PubMed, Embase, and Cochrane databases and included articles published from 1988 to 2017. Studies were included in this review if determined to be of high quality and containing injury data relevant to the levels of competition. Pertinent data regarding ice hockey injury epidemiology and prevention at various levels of competition were analyzed. STUDY DESIGN: Clinical review. LEVEL OF EVIDENCE: Level 4. RESULTS: Youth, high school, junior, college, and professional hockey players have unique injury patterns. Injuries occur much more often in a game compared with a practice, and injury risk increases with each level of competition. Preventative measures, such as mandatory facial protection and delayed body checking in games until age 13 years, are proven strategies to reduce the risk of facial injury and concussion. CONCLUSION: An understanding of common injury types and mechanisms according to age and level of play aids the clinician in diagnosis and management. This information can also guide preventative strategies in the areas of education, coaching, rule enforcement, rule modifications, equipment improvement, and sportsmanship.


Assuntos
Traumatismos em Atletas/epidemiologia , Hóquei/lesões , Traumatismos em Atletas/prevenção & controle , Concussão Encefálica/epidemiologia , Concussão Encefálica/prevenção & controle , Comportamento Competitivo/fisiologia , Contusões/epidemiologia , Contusões/prevenção & controle , Traumatismos Faciais/epidemiologia , Traumatismos Faciais/prevenção & controle , Fraturas Ósseas/epidemiologia , Fraturas Ósseas/prevenção & controle , Dispositivos de Proteção da Cabeça , Humanos , Incidência , Lacerações/epidemiologia , Lacerações/prevenção & controle , Entorses e Distensões/epidemiologia , Entorses e Distensões/prevenção & controle , Estados Unidos/epidemiologia
13.
Med Probl Perform Art ; 34(2): 102-104, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31152653

RESUMO

The anconeus muscle is a small and minor functioning muscle located at the posterolateral elbow region. It helps forearm extension and tightening of the joint capsule. Despite its limited functions, pathologies of the anconeus muscle can mimic other abnormalities of the elbow joint. Here, we report a rare case of a traumatic anconeus muscle contusion in a 15-year-old boy due to falling during dance. MRI showed contusion in the anconeus muscle, as well as strain in the ulnar collateral ligament and edema in the coronoid process of the ulna. To our knowledge, this is the first described case of traumatic anconeus muscle contusion in the literature. The presence of long-lasting lateral elbow pain in trauma cases without fracture should alert clinicians to consider anconeus muscle abnormalities. MRI is the best modality for diagnosis in these cases.


Assuntos
Contusões , Articulação do Cotovelo , Músculo Esquelético , Acidentes por Quedas , Adolescente , Dança , Cotovelo , Humanos , Masculino , Músculo Esquelético/lesões , Dor
14.
Phys Ther Sport ; 39: 38-43, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31238197

RESUMO

OBJECTIVES: To explore the epidemiological patterns and musculoskeletal characteristics of lower limb injuries in Major League Baseball (MLB). DESIGN: Descriptive epidemiological study. SETTING: The MLB was screened for injuries between 2010 and 2016, inclusive. PARTICIPANTS: Players that were placed on the Disabled List (DL) and had lower limb injuries. MAIN OUTCOME MEASURES: Our database entailed the player's position, injury location, injury cause, and time spent on the DL. Analysis of characteristics and mean incidence rates (MIR) were conducted, and P values<0.05 were considered significant (CI 95%). RESULTS: A total of 3546 injuries were recorded; 1053 injuries (29.7%) affected the lower limbs (n = 1053). On average, 150.4 players per season were placed on the DL for a mean of 45.7 days. Thighs were the most common site of injury, whereas hip injuries had the highest average days on DL (62 days). Fielders had a significantly higher MIR of lower leg injuries when compared to pitchers (P = 0.007) and catchers (P = 0.024), and a significantly higher MIR of foot injuries than that of pitchers (P = 0.035). April was the month with the highest number of injuries (224 injuries, 21.3%). Only 43(4%) injuries required surgical intervention. CONCLUSION: Lower limb injuries are increasing in the MLB. Understanding the neuromuscular patterns of injuries will help in extrapolating better prevention policies.


Assuntos
Traumatismos em Atletas/epidemiologia , Beisebol/lesões , Extremidade Inferior/lesões , Adulto , Contusões/epidemiologia , Fraturas Ósseas/epidemiologia , Humanos , Incidência , Masculino , Entorses e Distensões/epidemiologia , Estados Unidos/epidemiologia , Adulto Jovem
15.
Forensic Sci Med Pathol ; 15(4): 555-563, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31250256

RESUMO

The purpose of this study was to evaluate the ability of alternate light source illumination to enhance bruises in pigmented skin. Previous work was limited to simulating bruises in non-pigmented (Caucasoid type) skin by injecting blood into pigskin. In this study, it was investigated if adding a layer of melanin to the surface of the skin would simulate pigmented skin. The study included evaluating the use of a filter that transmitted infrared light (wavelength greater than 720 nm) in place of the recommended visible light filters for the alternate light sources. The results obtained using pigskin with a layer of melanin were almost the same as results using the naturally pigmented goat ear. This indicated adding a layer of melanin could be used as a model for pigmented skin in this simulation of fresh bruising. Comparing the pigskin without melanin with pigskin with melanin revealed that the optimal light source to enhance the appearance of bruising, simulated by injection of blood, changed from violet to blue-green. Using the infrared transmitting filter resulted in greater enhancement than using the alternate light sources with their recommended visible light filter. The advantage of using the infrared transmitting filter was greater with the pigskin coated with melanin and the naturally pigmented goat ears than in the non-pigmented pigskin, however, the results remain to be validated using real bruises in naturally pigmented human skin.


Assuntos
Contusões/patologia , Raios Infravermelhos , Luz , Fotografação , Pigmentação da Pele , Pele/patologia , Animais , Patologia Legal , Cabras , Melaninas/administração & dosagem , Modelos Animais , Pele/lesões , Suínos
16.
Fa Yi Xue Za Zhi ; 35(2): 154-159, 2019 Apr.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-31135108

RESUMO

Abstract: Objective To study the expressions of transforming growth factor-ß1 (TGF-ß1) and EⅢA-fibronectin (EⅢA-FN) at different time points of antemortem injury, antemortem injury postmortem expression and postmortem injury and to explore their application value in wound age estimation. Methods A model of rat skeletal muscle contusion was established. The rats were randomly divided into normal control group (n=5), antemortem contusion group (n=40), antemortem contusion postmortem expression group (n=110) and postmortem injury group (n=25). The expressions of TGF-ß1 and EⅢA-FN after rat skeletal muscles antemortem contusion were detected with immunohistochemical staining. Expression changes of TGF-ß1 and EⅢA-FN mRNA in each group were analyzed with real-time fluorescence quantitative PCR. Results Immunohistochemical staining results showed that a large number of polymorphonuclear leukocyte, mononuclear cells and fibroblastic cells showed a strong expression of TGF-ß1 in wounded zones 12 h-14 d after antemortem contusion. EⅢA-FN was mainly distributed in the extracellular matrix, 3 to 7 d post-traumatic. Real-time fluorescence quantitative PCR results showed that TGF-ß1 and EⅢA-FN mRNA in antemortem injury group reached the peak at 3 and 5 d post-traumatic respectively. The expressions of TGF-ß1 and EⅢA-FN mRNA in antemortem contusion postmortem expression group peaked at 6 h and 12 h postmortem. The expression of TGF-ß1 and EⅢA-FN mRNA in postmortem injury group 0.5-12 h postmortem was significantly lower than those of the normal control group and the antemortem contusion group. Conclusion TGF-ß1 and EⅢA-FN might become a reference index for skeletal muscle wound age estimation.


Assuntos
Contusões/metabolismo , Fibronectinas/genética , Músculo Esquelético/metabolismo , Fator de Crescimento Transformador beta1/genética , Animais , Biomarcadores/metabolismo , Fibroblastos , Fibronectinas/metabolismo , Músculo Esquelético/lesões , Mudanças Depois da Morte , Distribuição Aleatória , Ratos , Fator de Crescimento Transformador beta1/metabolismo
17.
Fa Yi Xue Za Zhi ; 35(2): 166-170, 2019 Apr.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-31135110

RESUMO

Abstract: Objective To explore the application of neutrophil migration distance for wound age estimation of skeletal muscles in rats, and to provide methodological basis for follow-up study in future. Methods The skeletal muscle contusion model was established in rats, and the control group and the 2, 4, 6 h post-traumatic groups were set. The law of response of neutrophils that participated in the inflammation after injury was detected by immunohistochemical staining, and the relationship between neutrophil migration distance and injury time was detected by TissueFAXS PLUS software. Results The skeletal muscle was obviously infiltrated with neutrophils 2-6 h after injury. The positive rate of neutrophil was (28.75±0.94)% at 2 h post-traumatic, and reached the peak (45.50±3.63)% at 4 h post-traumatic, then decreased to (31.92±1.56)% at 6 h post-traumatic. The neutrophil migration distances increased with the progress of inflammation, and reached (124.80±12.32) µm, (229.03±21.45) µm and (335.04±16.75) µm at 2 h, 4 h and 6 h, respectively. Conclusion There is a relationship of neutrophil infiltrated number and migration distance and wound age within the 2-6 h after skeletal muscle injury, which could be used for the inference of skeletal muscle wound age.


Assuntos
Contusões/metabolismo , Músculo Esquelético , Infiltração de Neutrófilos , Neutrófilos , Animais , Seguimentos , Músculo Esquelético/metabolismo , Músculo Esquelético/patologia , Ratos , Ratos Sprague-Dawley , Fatores de Tempo
18.
Fa Yi Xue Za Zhi ; 35(2): 160-165, 2019 Apr.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-31135109

RESUMO

Abstract: Objective To investigate the estimation of early and mid-term wound age by a combination of four mRNAs, the DNA polymerase delta-interacting protein 3 (POLDIP3) mRNA, regulator of chromosome condensation 1 like (RCC1L) mRNA, proline-rich 5 (PRR5) mRNA, and ribonucleic acid export 1 (RAE1) mRNA in rats skeletal muscles. Methods The model of rat skeletal muscle contusion was established, and then contusion area muscle tissue was extracted 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44 and 48 h after injury. Histomorphological changes during the repair process after rat skeletal muscle contusion were observed. The relative expressions of Poldip3, Rcc1l, Prr5 and Rae1 mRNAs were detected by reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR). Different stages of wound age were classified by using the expression patterns of four genes at various time points after injury. The accuracy of the results was verified by Fisher discriminant analysis. Results Histomorphological results showed that the repair process after skeletal muscle contusion occurred with the prolonging of time. Through combination of the expression trends of the four kinds of mRNAs, the 48 h after injury could be divided into three periods, 4-12 h, 16-28 h and 32-48 h. The Fisher discrimination method showed that the classification accuracy rates of the three periods were 83.3%, 75.0% and 73.3%, respectively. Conclusion The classification discrimination based on the relative expression of every gene has a higher accuracy, and the accuracy of wound age estimation with combination of mRNA relative expressions is higher than that with a single indicator. By combining with Fisher discrimination method, this method can be used for early and mid-term wound age estimation.


Assuntos
Contusões/metabolismo , Músculo Esquelético/lesões , Músculo Esquelético/metabolismo , RNA Mensageiro/genética , Animais , Ratos , Ratos Sprague-Dawley , Fatores de Tempo
20.
Ann Thorac Cardiovasc Surg ; 25(4): 185-191, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31068507

RESUMO

OBJECTIVE: To compare the effectiveness of surgical versus nonsurgical treatment for multiple rib fractures accompanied with pulmonary contusion. METHODS: The clinical records of consecutive 167 patients with multiple rib fractures accompanied with pulmonary contusion, who were treated from June 2014 to June 2017, were retrospectively analyzed. Of them, 75 and 92 underwent surgery (surgery group) and non-surgical treatment (non-surgery group), respectively. Patient pain score, complications, length of hospital stay, cost of hospitalization, and post-treatment 3-month follow-up results were compared. RESULTS: The mean number of days and moderate pain in the surgery group was significantly lower than that of the non-surgery group (p <0.01). The incidence of post-treatment complications was significantly lower in the surgery group than in the non-surgery group. The length of hospital stay of the surgery group was also significantly shorter than that of the non-surgery group (p <0.01). The cost of hospitalization was significantly higher in the surgery group than in the non-surgery group (p <0.01). The chest computed tomography (CT) scan which was performed 3 months after the treatment revealed that the surgery group had a better recovery than the non-surgery group. Physical recovery of the surgery group was also significantly better than that of the non-surgery group. CONCLUSION: Surgery to treat multiple rib fractures (≥ 4 fractures) accompanied with pulmonary contusion is safe and effective.


Assuntos
Contusões/terapia , Fixação de Fratura/métodos , Consolidação da Fratura , Fraturas Múltiplas/terapia , Lesão Pulmonar/terapia , Fraturas das Costelas/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Pesquisa Comparativa da Efetividade , Contusões/diagnóstico por imagem , Contusões/economia , Contusões/fisiopatologia , Feminino , Fixação de Fratura/efeitos adversos , Fixação de Fratura/economia , Fraturas Múltiplas/diagnóstico por imagem , Fraturas Múltiplas/economia , Fraturas Múltiplas/fisiopatologia , Custos Hospitalares , Humanos , Tempo de Internação , Lesão Pulmonar/diagnóstico por imagem , Lesão Pulmonar/economia , Lesão Pulmonar/fisiopatologia , Masculino , Pessoa de Meia-Idade , Dor Pós-Operatória/etiologia , Estudos Retrospectivos , Fraturas das Costelas/diagnóstico por imagem , Fraturas das Costelas/mortalidade , Fraturas das Costelas/fisiopatologia , Fatores de Tempo , Resultado do Tratamento , Adulto Jovem
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