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1.
Int J Radiat Oncol Biol Phys ; 106(3): 462, 2020 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-32014144
2.
Bratisl Lek Listy ; 121(1): 73-78, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31950843

RESUMO

AIM: The aim of the present study was to investigate the immunohistochemical expression of selected collagen types, namely collagen types I and V and procollagen type III in the renal parenchyma and interstitium and in the myocardium of spontaneously hypertensive rats. MATERIAL AND METHODS: For the present study, we used two age groups of 6- and 12-month-old spontaneously hypertensive rats. An immunohistochemical analysis was conducted with monoclonal antibodies against collagen types I and V and procollagen type III. A semi-quantitative analysis of immunostaining intensity was conducted with the Image J software. RESULTS: In the kidney, all three molecules showed higher expression at the age of 12 months, which was particularly notable for procollagen type III and collagen type V, which stained as highly-positive. In the myocardium, the immunoreactivity of collagen types I and V was stronger in 12-month-old animals, while that of procollagen type III did not change substantially. CONCLUSION: The present study suggests a role of collagen types III and V in hypertensive kidney disease, while also establishing the role of increased expression of collagen types I and V in adverse myocardial remodeling (Tab. 1, Fig. 2, Ref. 48).


Assuntos
Hipertensão , Rim , Miocárdio , Animais , Colágeno/metabolismo , Coração , Hipertensão/metabolismo , Hipertensão/fisiopatologia , Rim/metabolismo , Miocárdio/metabolismo , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Remodelação Vascular
3.
Life Sci ; 244: 117324, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31958420

RESUMO

AIMS: The aim of the present study was to evaluate the possible antioxidant role of oleic acid (OA) against Cd-induced injuries in the heart and liver tissues of male Wistar rats. MAIN METHODS: Rats were treated with either vehicle (control), or OA (10 mg/kg b.w., fed orally), or Cd (0.44 mg/kg b.w., s.c.), or both (OA + Cd) for 15 days. Following completion of the treatment period, biomarkers of organ damage and oxidative stress including ROS, activities of antioxidant enzymes and their level, activities of Krebs cycle enzymes and respiratory chain enzymes were measured. Levels of interleukins (IL-1ß, IL-6, IL-10), tumor necrosis factor (TNF-α) and nuclear factor kappa B (NFκB) were estimated to evaluate the state of inflammation. In addition, changes in mitochondrial membrane potential and status of cytochrome c (Cyt c) were also studied. KEY FINDINGS: Pre-treatment of rats with OA significantly protected against Cd-induced detrimental changes possibly by decreasing endogenous ROS through regulation of antioxidant defense system, inflammatory responses and activities of metabolic enzymes. Moreover, OA was also found to restore mitochondrial membrane potential possibly by regulating Cyt c leakage thereby increasing mitochondrial viability. SIGNIFICANCE: Our results for the first time demonstrated systematically that OA provided protection against Cd-induced oxidative stress mediated injuries in rat heart and liver tissues through its antioxidant mechanism. The results raise the possibility of using OA singly or in combination with other antioxidants or diet in the treatment of situations arising due to oxidative stress and may have future therapeutic relevance.


Assuntos
Ácido Oleico/metabolismo , Ácido Oleico/farmacologia , Animais , Antioxidantes/farmacologia , Biomarcadores/metabolismo , Cádmio/efeitos adversos , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Coração/efeitos dos fármacos , Traumatismos Cardíacos/prevenção & controle , Inflamação/patologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Miocárdio/metabolismo , Miocárdio/patologia , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Wistar
4.
Rev Med Suisse ; 16(679): 218, 2020 Jan 29.
Artigo em Alemão | MEDLINE | ID: mdl-31995312
5.
J Zoo Wildl Med ; 50(4): 903-909, 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31926522

RESUMO

Most of the agouti species are kept in captivity, including the species Dasyprocta azarae. These animals are of zootechnical interest and, in addition, they can potentially be used as experimentation models because of their physical characteristics and possibility of manipulation. Therefore, the purpose of this study was to investigate the feasibility of the echocardiographic exam in nonsedated agoutis and to determine the normal reference ranges for the standard transthoracic echocardiographic parameters in healthy, adult, free-ranging agoutis found in an urban wood and intended for scientific investigations. Most of the echocardiographic parameters evaluated were similar to what has already been described for other rodent species such as rabbits or the Dasyprocta primnolopha agoutis. Based on the information compiled in this study, echocardiographic examination is feasible in awake adult, free-ranging agoutis. The results obtained from the morphologic and hemodynamic evaluation of the heart can help in future studies, either involving the clinical aspects or considering the potential use of these animals as an experimental model.


Assuntos
Dasyproctidae/anatomia & histologia , Ecocardiografia/veterinária , Coração/diagnóstico por imagem , Animais , Dasyproctidae/fisiologia , Feminino , Coração/fisiologia , Masculino
6.
J Zoo Wildl Med ; 50(4): 976-982, 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31926531

RESUMO

Reptiles can suffer from infectious and noninfectious cardiac pathologies, requiring the need for standardized diagnostic approaches and reference intervals. Despite the popularity of ball pythons (Python regius) as pets, echocardiographic measurements are unknown in this species. Twenty healthy adult ball pythons were evaluated to identify imaging planes, establish reference intervals for cardiac assessment by two-dimensional echocardiography, and study the effects of sex, body length, and body mass on heart rate, fractional shortening, and vascular, atrial, and ventricular dimensions. Echocardiography was performed under manual restraint. Most cardiac measurements were positively correlated with body length and mass, with the strongest correlation between ventricular end-systolic measurements and body length. The only significant difference found between sexes was for right and left atrial lengths. This study provides guidelines and reference intervals for two-dimensional echocardiographic measurements in adult healthy ball pythons.


Assuntos
Boidae/anatomia & histologia , Ecocardiografia/veterinária , Coração/diagnóstico por imagem , Animais , Feminino , Masculino , Valores de Referência
7.
Life Sci ; 242: 117239, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31901444

RESUMO

AIMS: Reactive oxygen species (ROS) and pro-inflammatory cytokines play a critical role in organ damage induced by ethanol consumption. Interleukin (IL)-10 maintain tissue homeostasis through restriction of excessive inflammatory responses and inhibition of ROS generation. These responses limit unnecessary tissue damage in the cardiorenal system. We hypothesized that IL-10 would limit the deleterious effects induced by ethanol consumption in the cardiorenal system. MATERIALS AND METHODS: Male C57BL/6J wild-type (WT) or IL10-deficient mice (IL-10-/-) were treated with ethanol (20% v/v) for 6 weeks. KEY FINDINGS: IL-10 deficiency was associated with an increased mortality rate. Ethanol consumption decreased plasma levels of IL-10 in WT mice. Increased levels of IL-6 were detected in the aorta from IL-10-deficient mice, but not WT mice. No alterations in the levels of urea, creatinine, sodium, potassium or creatine kinase (CK)-MB were found after treatment with ethanol. Augmented concentration of thiobarbituric acid reactive substances (TBARS) was found in the left ventricle (LV) of IL-10-deficient mice, but not WT mice. Increased levels of superoxide anion (O2-) were found in the renal cortex of both WT and IL-10-deficient mice. Renal cortex from WT mice chronically treated with ethanol showed decreased levels of H2O2. No changes in the expression of Nox1, Nox4 or catalase were found in the renal cortex from ethanol-treated mice. SIGNIFICANCE: IL-10 limited the production of ROS and the synthesis of pro-inflammatory cytokines induced by ethanol in the cardiorenal system. These findings provided novel evidence that IL-10 counteracted the initial mechanisms whereby ethanol induces its cardiorenal damages.


Assuntos
Etanol/efeitos adversos , Coração/efeitos dos fármacos , Interleucina-10/metabolismo , Rim/efeitos dos fármacos , Lesão Renal Aguda/induzido quimicamente , Animais , Western Blotting , Creatina Quinase Forma MB/sangue , Creatinina/sangue , Interleucina-10/sangue , Interleucina-10/fisiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Potássio/sangue , Sódio/sangue , Ureia/sangue
9.
Life Sci ; 240: 117107, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31785241

RESUMO

BACKGROUND: Toll-like receptor 4 (Tlr4) is recognized due to its role in the immune response. Also, this protein can participate in the signaling pathway of events triggered by physical exercise such as apoptosis, inflammation, and endoplasmic reticulum (ER) stress. The main objective of this study was to evaluate the role of Tlr4 in the markers of these events in the myocardium of mice submitted to acute physical exercise (APE) protocols at different intensities. METHODS: Echocardiogram, RT-qPCR, and immunoblotting technique were used to evaluate the left ventricle of wild-type (WT) and Tlr4 knockout (Tlr4 KO) submitted to APE protocols at 45, 60, and 75% of their maximal velocity. Also, we performed the bioinformatics analysis to establish the connection of heart mRNA levels of Tlr4 with heart genes of inflammation and ER stress of several isogenic strains of BXD mice. RESULTS: Under basal conditions, the Tlr4 deletion diminished the performance, and expression of inflammation and ER stress genes in the left ventricle, but increased the serum levels of CK, Il-17, and Tnf-alpha. Under the same exercise conditions, the Tlr4 deletion reduced the glycemia, serum levels of CK, Il-17, and Tnf-alpha, as well as genes and/or proteins related to apoptosis, inflammation and ER stress in the left ventricle, but increased the levels of CK-mb and LDH, as well as other genes related to apoptosis, inflammation, and ER stress in the left ventricle. CONCLUSION: Altogether, the current findings highlighted the effects of different acute exercise intensities were attenuated in the heart of Tlr4 KO mice.


Assuntos
Apoptose/fisiologia , Estresse do Retículo Endoplasmático/fisiologia , Coração/fisiologia , Inflamação , Esforço Físico/fisiologia , Receptor 4 Toll-Like/fisiologia , Animais , Apoptose/genética , Biologia Computacional , Creatina Quinase/sangue , Ecocardiografia , Estresse do Retículo Endoplasmático/genética , Coração/diagnóstico por imagem , Interleucina-17/sangue , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Esforço Físico/genética , Transdução de Sinais/fisiologia , Receptor 4 Toll-Like/genética , Fator de Necrose Tumoral alfa/sangue
10.
Chem Commun (Camb) ; 56(3): 415-418, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31821393

RESUMO

We report a novel 1,2-catechol based radioiodinated precursor for radioiodination of bicyclo[6.1.0]nonyne (BCN) installed biologically active molecules using a strain-promoted oxidation-controlled cyclooctyne-1,2-quinone cycloaddition reaction (SPOCQ) under ambient conditions. Compared to the reported methodologies, the new strategy demonstrates some clear advantages, including high in vitro and in vivo stability, high radiochemical yield, and exceptionally fast reaction kinetics at micro-molar concentration.


Assuntos
Reação de Cicloadição , Quinonas/química , Compostos Radiofarmacêuticos/química , Animais , Compostos Bicíclicos com Pontes/síntese química , Compostos Bicíclicos com Pontes/química , Catecóis/química , Coração/diagnóstico por imagem , Radioisótopos do Iodo/química , Marcação por Isótopo , Cinética , Camundongos , Camundongos Endogâmicos ICR , Oxirredução , Compostos Radiofarmacêuticos/síntese química , Tomografia Computadorizada com Tomografia Computadorizada de Emissão de Fóton Único
12.
Mar Genomics ; 49: 100701, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31451352

RESUMO

The zebrafish has proven to be an excellent organism for manipulation of its genome from a long history of transcript down-regulation using morpholino oligimers to more recent genome editing tools such as CRISPR-Cas9. Early forward and reverse genetic screens significantly benefited from the transparency of zebrafish embryos, allowing cardiac development as a function of genetics to be directly observed. However, gradual loss of transparency with subsequent maturation limited many of these approaches to the first several days post-fertilization. As many genes are developmentally regulated, the immature phenotype is not entirely indicative of that of the mature zebrafish. For accurate phenotyping, subsequent developmental stages including full maturation must also be considered. In adult zebrafish, cardiac function can now be studied in great detail due both to the size of the hearts as well as recent technological improvements. Because of their small size, zebrafish are particularly amenable to high frequency echocardiography for detailed functional recordings. Although relatively small, the hearts are easily excised and contractile parameters can be measured from whole hearts, heart slices, individual cardiomyocytes and even single myofibrils. Similarly, electrical activity can also be measured using a variety of techniques, including in vivo and ex vivo electrocardiograms, optical mapping and traditional microelectrode techniques. In this report, the major advantages and technical considerations of these physiological tools are discussed.


Assuntos
Coração/fisiologia , Peixe-Zebra/fisiologia , Animais , Células Cultivadas , Ecocardiografia , Técnicas In Vitro , Microeletrodos , Miócitos Cardíacos/fisiologia , Fenótipo , Imagens com Corantes Sensíveis à Voltagem
17.
Life Sci ; 242: 117225, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31881229

RESUMO

AIMS: Data concerning the influence of statin lipophilicity on the myotoxic and pleiotropic effects of statins is conflicting, and mechanistic head-to-head comparison studies evaluating this parameter are limited. In order to address the disparity, this mechanistic investigation aimed to assess the effects of two short-acting statins with different lipophilic indices on skeletal, cardiac and vascular smooth muscle physiology. MATERIALS AND METHODS: Young female Wistar rats were randomised to simvastatin (80 mg kg-1 day-1), pravastatin (160 mg kg-1 day-1) or control treatment groups. Changes in functional muscle performance were assessed, as well as mRNA levels of genes relating to atrophy, hypertrophy, mitochondrial function and/or oxidative stress. KEY FINDINGS: There were no significant differences in the mRNA profiles of isolated skeletal muscles amongst the treatment groups. In terms of skeleletal muscle performance, simvastatin reduced functionality but treatment with pravastatin significantly improved force production. Rodents given simvastatin demonstrated comparable myocardial integrity to the control group. Conversely, pravastatin reduced left ventricular action potential duration, diastolic stiffness and Mhc-ß expression. Pravastatin improved endothelium-dependent relaxation, particularly in muscular arteries, but this effect was absent in the simvastatin-treated rats. The responsiveness of isolated blood vessels to noradrenaline also differed between the statin groups. The findings of this study support that the effects of statins on skeletal, cardiac and vascular smooth muscle vary with their lipophilic indices. SIGNIFICANCE: The results of this work have important implications for elucidating the mechanisms responsible for the myotoxic and pleiotropic effects of statins.


Assuntos
Coração/efeitos dos fármacos , Inibidores de Hidroximetilglutaril-CoA Redutases/farmacologia , Músculo Esquelético/efeitos dos fármacos , Músculo Liso Vascular/efeitos dos fármacos , Pravastatina/farmacologia , Sinvastatina/farmacologia , Animais , Feminino , Microeletrodos , Contração Muscular/efeitos dos fármacos , Músculo Esquelético/metabolismo , Músculo Liso Vascular/metabolismo , Miocárdio/metabolismo , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transcriptoma/efeitos dos fármacos , Função Ventricular Esquerda/efeitos dos fármacos
19.
Br J Radiol ; 93(1105): 20190596, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31625759

RESUMO

OBJECTIVE: This study aimed to compare the plan quality of non-coplanar partial arc (NPA) volumetric modulated arc therapy (VMAT) to that of coplanar partial arc (CPA) VMAT for stereotactic ablative radiotherapy (SABR) for lung cancer. METHODS: A total of 20 patients treated for lung cancer with the SABR VMAT technique and whose lung tumors were close to the heart were retrospectively selected for this study. For the CPA VMAT, three coplanar half arcs were used while two coplanar half arcs and one noncoplanar arc rotating 315°-45° with couch rotations of 315° ± 5° were used for the NPA VMAT. For each patient, identical CT image sets and identical structures were used for both the CPA and NPA VMAT plans. Dose-volumetric parameters of each plan were analyzed. RESULTS: For the planning target volume and both lungs, no statistically significant differences between the CPA and NPA VMAT plans were observed in general. For the heart, average values of D0.1cc of the CPA and NPA VMAT plans were 29.42 ± 13.37 and 21.71 ± 9.20 Gy, respectively (p < 0.001). For whole body, the mean dose and the gradient index of the CPA VMAT plans were 1.2 ± 0.5 Gy and 4.356 ± 0.608 while those of the NPA VMAT plans were 1.1 ± 0.5 Gy and 4.111 ± 0.480, respectively (both with p < 0.001). CONCLUSION: The NPA VMAT proposed in this study showed more favorable plan quality than the CPA VMAT plans for lung SABR with tumors located close to the heart. ADVANCES IN KNOWLEDGE: For lung SABR, NPA VMAT can reduce doses to the heart as well as whole-body irradiation.


Assuntos
Coração/efeitos da radiação , Neoplasias Pulmonares/radioterapia , Radiocirurgia/métodos , Planejamento da Radioterapia Assistida por Computador/métodos , Radioterapia de Intensidade Modulada/métodos , Algoritmos , Anisotropia , Fracionamento da Dose de Radiação , Humanos , Órgãos em Risco/efeitos da radiação , Dosagem Radioterapêutica , Estudos Retrospectivos , Tomografia Computadorizada por Raios X
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