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1.
Gene ; 766: 145022, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32758579

RESUMO

BACKGROUND: A better understanding of the mechanism(s) underlying cardioembolic stroke can promote recovery and reduce the risk of recurrent embolisms. METHODS: Peripheral blood mononuclear cell (PBMC) gene expression datasets from cardioembolic patients and healthy controls were obtained from the Gene Expression Omnibus (GEO) database (GSE58294). The Limma software package was utilized to identify differentially-expressed genes (DEGs). Protein-protein interaction (PPI) analysis of the DEGs was performed using STRING. A weighted gene co-expression network analysis (WGCNA) was used to build a gene co-expression network. In vitro experiments assessed the effects on neutrophils exposed to oxygen and glucose-deprived (OGD) cortical neurons. An in vivo murine model of thromboembolic stroke was constructed through thrombin injection to examine effects on circulating neutrophils. Mechanistic in vitro studies were conducted using the proteasome inhibitor MG132, the p53-Mdm2 binding inhibitor Nutlin-3a, Mdm2 small-interfering RNA (siRNA), and Ctnnb1 siRNA. RESULTS: DEG analysis identified 44 upregulated and 66 downregulated genes in cardioembolic stroke PBMCs. PPI analysis of these DEGs yielded one eight-node protein module with ß-catenin (CTNNB1) as the central hub protein. Integration of the DEGs with WGCNA-derived hub genes revealed the key hub DEGs CTNNB1 and mouse double minute 2 (MDM2). Follow-up experiments revealed Mdm2, p53, and phospho-ß-catenin upregulation in neutrophils exposed to OGD neurons in vitro and following thromboembolic stroke in vivo. Mechanistic studies revealed that neutrophils transcriptionally upregulate Ctnnb1 expression to compensate for Mdm2/p53-mediated ß-catenin degradation induced by exposure to OGD neurons, thereby promoting neutrophil survival. CONCLUSION: Compensatory Ctnnb1 transcriptional upregulation in neutrophils induced by ischemic neuron exposure may be involved in promoting neutrophil survival following cardioembolic stroke.


Assuntos
Neutrófilos/metabolismo , Proteínas Proto-Oncogênicas c-mdm2/genética , Acidente Vascular Cerebral/genética , Acidente Vascular Cerebral/metabolismo , Proteína Supressora de Tumor p53/genética , Regulação para Cima/genética , beta Catenina/genética , Animais , Células Cultivadas , Regulação para Baixo/genética , Expressão Gênica/genética , Redes Reguladoras de Genes/genética , Coração/fisiopatologia , Humanos , Leucócitos Mononucleares/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Neurônios/metabolismo , Transcrição Genética/genética , Ativação Transcricional/genética
2.
Gene ; 766: 145154, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32949699

RESUMO

CircRNA serves a crucial role in the development of heart failure (HF). Nevertheless, the regulatory mechanisms of circ_0062389 in HF are unknown. This study aims to examine the effect and mechanism of circ_0062389 on cardiomyocyte apoptosis in HF rats and H9C2 cells. Rats were divided into 5 groups (n = 8/group): the Control group, Sham group, HF group, HF + si-NC group, and HF + si-circRNA group. The echocardiography was used to examine the cardiac function, including LVIDd, LVIDs, IVSd, and IVSs. The apoptosis of myocardial tissue was detected through TUNEL method. H9C2 cells were randomly assigned into Control group (untransfected H9C2 cells), H/R group (untransfected H/R H9C2 cells), H/R + si-NC group (transfected si-NC) and H/R + si-circRNA group (transfect si-circ_0062389). Cell apoptosis was assessed through flow cytometry. The expression of circ_0062389 in myocardial tissues of HF rats was significantly higher than that of Control group and Sham group. Silencing circ_0062389 significantly reduced the levels of LVIDd, LVIDs, IVSd, and IVSs. Additionally, silencing circ_0062389 could significantly reduce the apoptosis rate of rat cardiomyocytes. Besides, silencing circ_0062389 significantly reduced the expression of TGF-ß1 and Smad3 protein. Silencing circ_0062389 could alleviate cardiomyocyte apoptosis in HF rats via modulating TGF-ß1/Smad3 signaling pathway, which might be a promising target for the treatment of HF.


Assuntos
Apoptose/genética , Insuficiência Cardíaca/genética , Miócitos Cardíacos/patologia , Interferência de RNA/fisiologia , RNA Circular/genética , Transdução de Sinais/genética , Proteína Smad3/genética , Fator de Crescimento Transformador beta1/genética , Animais , Linhagem Celular , Coração/fisiologia , Insuficiência Cardíaca/patologia , Masculino , Miocárdio/patologia , Ratos , Ratos Sprague-Dawley
3.
Kyobu Geka ; 73(10): 749-756, 2020 Sep.
Artigo em Japonês | MEDLINE | ID: mdl-33130760

RESUMO

Managing patients with concurrent malignant neoplasms and cardiovascular disease is an important issue, especially with aging populations;however, the optimal treatment strategy in these patients remains controversial. We report 27 patients with simultaneous cardiac and malignant disease in our institution over the past 12 years;23 patients underwent cardiovascular surgery, 4 patients underwent cardiovascular surgery after treatment for malignant disease, and 3 patients died of malignant disease. The treatment strategy for patients with concurrent cardiac and malignant disease should be chosen according to the severity of the cardiovascular disease and the expected prognosis of the malignant disease.


Assuntos
Neoplasias , Coração , Humanos , Neoplasias/complicações , Neoplasias/cirurgia , Prognóstico
4.
Kyobu Geka ; 73(10): 757-763, 2020 Sep.
Artigo em Japonês | MEDLINE | ID: mdl-33130761

RESUMO

The lower preoperative left ventricular ejection fraction( LVEF), the more postoperative death. The perioperative management for cardiovascular patients with heart failure (LVEF<40%) is of great importance in cardiac surgery. The failing heart is characterized by intracellular Ca2+ handling abnormalities during excitation/contraction coupling( i.e., less amount of cytosolic Ca2+ recruitment in systole and insufficient cytosolic Ca2+ extrusion in diastole), which are caused by increased reverse-mode Na+/ Ca2+ exchange activity and abnormal sarcoplasmic reticular Ca2+ channels (ryanodine receptors) and Ca2+ pumps (adenosine triphosphatase:ATPases). Myocardial ischemia/reperfusion (I/R) damage is characterized by intracellular acidosis followed by Ca2+ overload during I/R. The failing/hypertrophied myocardium has a low coronary vascular density, leading to low oxygen supply to the cardiomyocyte, and is vulnerable to Ca2+ load during I/R. Based on those abnormalities, hypothermic cardioplegia is recommended to suppress myocardial oxygen demand in open heart surgery for patients with heart failure(low LVEF). Optimal medical managements using adrenergic stimulators, vasodilators, antiarrhythmics, cardiac pacing, NO inhalation, or myocardial Ca2+ sensitizers under preload adjustment may be essential for hemodynamic improvement of postoperative low cardiac output syndrome. On a case-bycase basis, mechanical circulatory support systems should be utilized before the development of multiple organ failure.


Assuntos
Procedimentos Cirúrgicos Cardíacos , Insuficiência Cardíaca , Cálcio/metabolismo , Coração , Insuficiência Cardíaca/complicações , Insuficiência Cardíaca/terapia , Humanos , Retículo Sarcoplasmático/metabolismo , Trocador de Sódio e Cálcio , Volume Sistólico , Função Ventricular Esquerda
7.
Monaldi Arch Chest Dis ; 90(4)2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33169595

RESUMO

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection continues to be a public health emergency and a pandemic of international concern. As of April 31st,  the reported cases of COVID-19 are three million in 186 countries. Reported case fatality has crossed 200 thousand among which more than fifty thousand has been in the USA. Most patients present with symptoms of fever, cough, and shortness of breath following exposure to other COVID-19 patients. Respiratory manifestations predominate in patients with mild, moderate, severe illness. Imaging of patients with COVID-19 consistently reports various pulmonary parenchymal involvement. In this article we wanted to reinforce and review the various reported imaging patterns of cardiac and mediastinal involvement in COVID-19 patients. Among patients with COVID 19 who underwent various imaging of chest various cardiac findings including pericardial effusion, myocarditis, cardiomegaly has been reported. Most of these findings have been consistently reported in patients with significant acute myocardial injury, and fulminant myocarditis. Acute biventricular dysfunction has also been reported with subsequent improvement of the same following clinical improvement. Details of cardiac MRI is rather limited. In a patient with clinical presentation of acute myocarditis, biventricular myocardial interstitial edema, diffuse biventricular hypokinesia, increased ventricular wall thickness, and severe LV dysfunction has been reported. Among patients with significant clinical improvement in LV structure and function has also been documented. With increasing number of clinical cases, future imaging studies will be instrumental in identifying the various cardiac manifestations, and their relation to clinical outcome.


Assuntos
Cardiomegalia/diagnóstico por imagem , Infecções por Coronavirus/diagnóstico por imagem , Coração/diagnóstico por imagem , Isquemia Miocárdica/diagnóstico por imagem , Miocardite/diagnóstico por imagem , Derrame Pericárdico/diagnóstico por imagem , Pneumonia Viral/diagnóstico por imagem , Disfunção Ventricular Esquerda/diagnóstico por imagem , Betacoronavirus , Cardiomegalia/fisiopatologia , Angiografia Coronária , Infecções por Coronavirus/fisiopatologia , Ecocardiografia , Edema/diagnóstico por imagem , Edema/fisiopatologia , Coração/fisiopatologia , Humanos , Imagem por Ressonância Magnética , Isquemia Miocárdica/fisiopatologia , Miocardite/fisiopatologia , Pandemias , Derrame Pericárdico/fisiopatologia , Pneumonia Viral/fisiopatologia , Radiografia Torácica , Recuperação de Função Fisiológica , Tomografia Computadorizada por Raios X , Disfunção Ventricular/diagnóstico por imagem , Disfunção Ventricular/fisiopatologia , Disfunção Ventricular Esquerda/fisiopatologia
11.
Nat Commun ; 11(1): 5555, 2020 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-33144559

RESUMO

It is highly debated how cyclic adenosine monophosphate-dependent regulation (CDR) of the major pacemaker channel HCN4 in the sinoatrial node (SAN) is involved in heart rate regulation by the autonomic nervous system. We addressed this question using a knockin mouse line expressing cyclic adenosine monophosphate-insensitive HCN4 channels. This mouse line displayed a complex cardiac phenotype characterized by sinus dysrhythmia, severe sinus bradycardia, sinus pauses and chronotropic incompetence. Furthermore, the absence of CDR leads to inappropriately enhanced heart rate responses of the SAN to vagal nerve activity in vivo. The mechanism underlying these symptoms can be explained by the presence of nonfiring pacemaker cells. We provide evidence that a tonic and mutual interaction process (tonic entrainment) between firing and nonfiring cells slows down the overall rhythm of the SAN. Most importantly, we show that the proportion of firing cells can be increased by CDR of HCN4 to efficiently oppose enhanced responses to vagal activity. In conclusion, we provide evidence for a novel role of CDR of HCN4 for the central pacemaker process in the sinoatrial node.


Assuntos
Relógios Biológicos , AMP Cíclico/metabolismo , Canais Disparados por Nucleotídeos Cíclicos Ativados por Hiperpolarização/metabolismo , Nó Sinoatrial/patologia , Potenciais de Ação/efeitos dos fármacos , Animais , Arritmias Cardíacas/complicações , Arritmias Cardíacas/patologia , Relógios Biológicos/efeitos dos fármacos , Pressão Sanguínea/efeitos dos fármacos , Bradicardia/complicações , Bradicardia/patologia , Carbacol/farmacologia , Eletrocardiografia , Feminino , Células HEK293 , Coração/efeitos dos fármacos , Coração/fisiopatologia , Frequência Cardíaca/efeitos dos fármacos , Humanos , Camundongos Endogâmicos C57BL , Subunidades Proteicas/metabolismo , Reprodutibilidade dos Testes , Nó Sinoatrial/fisiopatologia , Nervo Vago/efeitos dos fármacos , Nervo Vago/fisiopatologia
13.
Stud Health Technol Inform ; 273: 255-257, 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-33087622

RESUMO

Smart devices, including the popular smart watches, often collect information on the heart beat rhythm and transmit it to a central server for storage or further processing. A factor introducing important limitations in the amount of data collected, transmitted and finally processed is the life of the mobile device or smart watch battery. Some devices choose to transmit the mean heart rate over relatively long periods of time, to save power. Heart Rate Variability (HRV) analysis gives useful information about the human heart, by only examining the heart rate time series. Its discriminating capability is affected by the amount of available information to process. Ideally, the whole RR interval time series should be used. We investigate here how this discriminating capability is affected, when the analysis is based on mean heart rate values transmitted over relatively long time periods. We show that we still can get useful information and the discriminating power is still remarkable, even when the amount of the available data is relatively small.


Assuntos
Coração , Frequência Cardíaca , Humanos
14.
Wiad Lek ; 73(8): 1712-1716, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33055339

RESUMO

OBJECTIVE: The aim: Study of the patterns of structural changes in the left ventricular myocardial capillaries of rats with arterial hypertension with combined pharmacotherapy with Bisoprolol and Thiotriazolinum. PATIENTS AND METHODS: Materials and methods: Experiments were conducted on 30 line rats with congenital stress-induced arterial hypertension: 10 animals without treatment and 10 animals with treatment. Pharmacological correction of spontaneous arterial hypertension was performed with 20 mg / kg of Bisoprolol and 50 mg / kg of Thiotriazolinum per os once a day. Pharmacotherapy began at 5 months of age, that is, at a time when compensated heart failure was formed in rats with arterial hypertension. Animals were withdrawn from the experiment 100 days after the start of the correction. Control was provided by intact animals (10 rats) of the corresponding age. While extracted from the experiment rats of all experimental groups had their arterial pressure measured using a plethysmograph, electron microscopic examination of the left ventricular myocardium and morphometric study of volumetric and quantitative densities, cross-section area and form factor of micropinocytotic vesicles were conducted. RESULTS: Results: In rats with arterial hypertension after application of Bisoprolol and Thiotriazolinum, arterial pressure significantly decreases in experimental rats compared to animals without correction. The number of capillaries in the myocardium after pharmacotherapy increases up to control values, which shows their reparation. In most endothelial cells, organelles retain their integrity and presence that are characteristic of intact rats. The well-expressed processes of transcytosis are shown by the statistical similarity of the quantitative density and the size of the micropinocytotic vesicles in the endothelial cells of the myocardium capillaries of compared experimental animals. CONCLUSION: Conclusions: In rats with arterial hypertension, the combination of Bisoprolol and Thiotriazolinum prevents the decrease in the number of capillaries in the myocardium of the left ventricle, promotes the preservation of the ultrastructure of their endothelial cells and maintains the processes of transedothelial transfer of substances at the level of intact animals.


Assuntos
Células Endoteliais , Hipertensão , Animais , Bisoprolol/uso terapêutico , Coração , Hipertensão/complicações , Hipertensão/tratamento farmacológico , Miocárdio , Ratos
17.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 221-224, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33017969

RESUMO

Non-invasive health monitoring has the potential to improve the delivery and efficiency of medical treatment. OBJECTIVE: This study was aimed at developing a neural network to classify the lung volume state of a subject (i.e. high lung volume (HLV) or low lung volume (LLV), where the subject had fully inhaled or exhaled, respectively) by analyzing cardiac cycles extracted from vibrational cardiography (VCG) signals. METHODS: A total of 15619 cardiac cycles were recorded from 50 subjects, of which 9989 cycles were recorded in the HLV state and the remaining 5630 cycles were recorded in the LLV state. A 1D convolutional neural network (CNN) was employed to classify the lung volume state of these cardiac cycles. RESULTS: The CNN model was evaluated using a train/test split of 80/20 on the data. The developed model was able to correctly classify the lung volume state of 99.4% of the testing data. CONCLUSION: VCG cardiac cycles can be classified based on lung volume state using a CNN. SIGNIFICANCE: These results provide evidence of a correlation between VCG and respiration volume, which could inform further analysis into VCG-based cardio-respiratory monitoring.


Assuntos
Algoritmos , Redes Neurais de Computação , Coração/diagnóstico por imagem , Pulmão/diagnóstico por imagem , Vibração
18.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 485-488, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018033

RESUMO

Utilizing Impulse Radio Ultra-WideBand (IR-UWB) radar for vital sign monitoring has attracted growing interest due to the noncontact measurement without privacy concerns. Most of existing researches assume that the subject's chest is directed to the radar antenna, to ensure the strength of backscattered signals from chest movement. However, a large angle between the antenna and the subject's chest caused by the body orientation badly affects the monitoring accuracy. Multiple observations of the same cardiopulmonary activity from different orientations provide more available measurements. This paper addresses the challenge by using an IR-UWB radar network instead of a single radar. Three IR-UWB radars are placed as endpoints of an equilateral triangle to collect vital sign information of a subject sitting at the center. A Conditional Generative Adversarial Network (CGAN) method is proposed to fuse multisensory data. First, the body orientation is classified by combining signal features and a random forest classifier. Then the impact of different angles on vital sign monitoring results is discussed and validated in each orientation. The data fusion process is modelled as an extended generative network with orientation based condition to produce the enhanced vital signal. This signal is optimized with the discriminator network to a fitted sinusoidal wave with heartbeat and respiratory information. Experimental results on measuring Heartbeat Rate (HR) in different orientations reveal the effectiveness and stability of the proposed method.


Assuntos
Radar , Processamento de Sinais Assistido por Computador , Coração , Frequência Cardíaca , Respiração
19.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 549-552, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018048

RESUMO

Quantification of directed (nonlinear) brain-heart interactions has turned to be an emerging topic of research and is important for the better understanding of central autonomic processing during specific diseases such as schizophrenia. Convergent Cross Mapping (CCM) was able to provide directed, frequency-selective and topographic views on existent interaction pattern of those patients. Investigations of the influence of individual heart rate (HR) on CCM estimations may further contribute to this topic. Relationship of mean HR and CCM was analyzed in a group of schizophrenic patients (N=17) and healthy controls (N=21). Influence of individual HR values was most pronounced for patients, for interactions from brain to heart and for the subgroup of patients with highest mean HR values.


Assuntos
Encéfalo , Esquizofrenia , Sistema Nervoso Autônomo , Coração , Frequência Cardíaca , Humanos
20.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 557-560, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018050

RESUMO

We propose a novel computational framework for the estimation of functional directional brain-to-heart interplay in an instantaneous fashion. The framework is based on inhomogeneous point-process models for human heartbeat dynamics and employs inverse-Gaussian probability density functions characterizing the timing of R-peak events. The instantaneous estimation of the functional directional coupling is based on the definition of point-process transfer entropy, which is here retrieved from heart rate variability (HRV) and Electroencephalography (EEG) power spectral series gathered from 12 healthy subjects undergoing significant sympathovagal changes induced by a cold-pressor test. Results suggest that EEG oscillations dynamically influence heartbeat dynamics with specific time delays in the 30-60s and 90-120s ranges, and through a functional activity over specific cortical regions.


Assuntos
Encéfalo , Coração , Eletroencefalografia , Frequência Cardíaca , Humanos , Projetos Piloto
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