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1.
Analyst ; 144(22): 6681-6688, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31599280

RESUMO

The anticancer mechanism of NO is difficult to study owing to its short lifetime and high reactivity. Thus, a theranostic anticancer NO donor assembled with NO on-demand release abilities, accurate lysosome location capabilities and signal feedback behavior was developed. Profiting from the theranostic properties, the specific mechanism was comprehensively studied. Spectral and cell imaging studies revealed that the as prepared NO donors could release NO in solution or within cancer cells. Fluorescence co-dyeing experiments demonstrated that Mo-Nap-NO entered lysosomes specifically and disrupted them after being triggered by light. Upon irradiation with 460 nm visible light, both the donors demonstrated considerable in vitro anticancer effects. A further mechanistic study showed that after entering the lysosome and being triggered by 460 nm irradiation, NO ruptured the lysosome, resulting in the release of cathepsin D into the cytosol, which activated the caspase3 mediated apoptosis pathway.


Assuntos
Antineoplásicos/farmacologia , Corantes Fluorescentes/farmacologia , Lisossomos/metabolismo , Naftalimidas/farmacologia , Doadores de Óxido Nítrico/farmacologia , Nitrosaminas/farmacologia , Antineoplásicos/efeitos da radiação , Linhagem Celular Tumoral , Fluorescência , Corantes Fluorescentes/efeitos da radiação , Humanos , Luz , Naftalimidas/efeitos da radiação , Doadores de Óxido Nítrico/efeitos da radiação , Nitrosaminas/efeitos da radiação , Nanomedicina Teranóstica/métodos
2.
Chem Commun (Camb) ; 55(73): 10920-10923, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31441463

RESUMO

The development of new NIR-II fluorophores, particularly those with facile syntheses, high fluorescence quantum yields, and stable and tunable photophysical properties, is challenging. Herein, we report a new class of small molecular NIR-II fluorophores based on aza-dipyrromethene boron difluoride (aza-BODIPY) dyes. We demonstrate promising photophysical properties of these dyes, such as large Stokes shift, superior photostability, and good fluorescence brightness as nanoparticles in aqueous solution. Because of these properties and high resolution and deep penetration NIR-II imaging ability, the aza-BODIPY based dyes show great potential as NIR-II imaging agents.


Assuntos
Compostos de Boro/química , Corantes Fluorescentes/química , Imagem Óptica/métodos , Pirróis/química , Animais , Compostos de Boro/síntese química , Compostos de Boro/efeitos da radiação , Compostos de Boro/toxicidade , Linhagem Celular Tumoral , Teoria da Densidade Funcional , Desenho de Drogas , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Corantes Fluorescentes/toxicidade , Raios Infravermelhos , Camundongos , Modelos Químicos , Nanopartículas/química , Poloxâmero/química , Pirróis/síntese química , Pirróis/efeitos da radiação , Pirróis/toxicidade
4.
Nat Commun ; 10(1): 3089, 2019 07 12.
Artigo em Inglês | MEDLINE | ID: mdl-31300649

RESUMO

Dual-emissive systems showing color-specific photoswitching are promising in bioimaging and super-resolution microscopy. However, their switching efficiency has been limited because a delicate manipulation of all the energy transfer crosstalks in the systems is unfeasible. Here, we report a perfect color-specific photoswitching, which is rationally designed by combining the complete off-to-on fluorescence switching capability of a fluorescent photochromic diarylethene and the frustrated energy transfer to the other fluorescent dye based on the excited-state intramolecular proton transfer (ESIPT) process. Upon alternation of UV and visible light irradiations, the system achieves 100% switching on/off of blue emission from the diarylethene while orange emission from the ESIPT dye is unchanged in the polymer film. By fabricating this system into biocompatible polymer nanoparticles, we demonstrate microscopic imaging of RAW264.7 macrophage cells with reversible blue-color specific fluorescence switching that enables super-resolution imaging with a resolution of 70 nm.


Assuntos
Transferência de Energia/efeitos da radiação , Corantes Fluorescentes/química , Microscopia Intravital/métodos , Imagem Molecular/métodos , Nanopartículas/química , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/efeitos da radiação , Cor , Fluorescência , Corantes Fluorescentes/efeitos da radiação , Luz , Camundongos , Microscopia Eletrônica de Transmissão/métodos , Microscopia de Fluorescência/métodos , Nanopartículas/efeitos da radiação , Polímeros/química , Polímeros/efeitos da radiação , Células RAW 264.7
5.
Chem Commun (Camb) ; 55(58): 8402-8405, 2019 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-31257385

RESUMO

A proof-of-principle for the application of hemi-indigo derivatives as RNA binders with photocontrollable fluorescence is presented. The photoswitch binds to the human immunodeficiency virus type 1 (HIV-1) RNA with a significant light-up effect. The fluorescence of the RNA-bound ligand can be reversibly switched ON and OFF by light without destroying the ligand-RNA associates.


Assuntos
Corantes Fluorescentes/metabolismo , HIV-1/genética , Indóis/metabolismo , RNA/metabolismo , Fluorescência , Corantes Fluorescentes/química , Corantes Fluorescentes/efeitos da radiação , Indóis/química , Indóis/efeitos da radiação , Ligantes , Luz , Estudo de Prova de Conceito , RNA/genética , Elementos de Resposta , Estereoisomerismo
6.
Chem Commun (Camb) ; 55(59): 8583-8586, 2019 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-31274135

RESUMO

We present a near-infrared (NIR) fluorescent probe, NR-HNO, which was successfully applied to visualizing H2S/NO "crosstalk" by the fluorescence detection of nitroxyl with a fast response time (5 min) and a large Stokes shift (131 nm) in living cells and tissue; it was also used to image nitroxyl in live mice.


Assuntos
Compostos de Benzilideno/química , Corantes Fluorescentes/química , Sulfeto de Hidrogênio/metabolismo , Óxido Nítrico/metabolismo , Óxidos de Nitrogênio/análise , Animais , Compostos de Benzilideno/efeitos da radiação , Linhagem Celular Tumoral , Corantes Fluorescentes/efeitos da radiação , Humanos , Rim/metabolismo , Luz , Limite de Detecção , Fígado/metabolismo , Camundongos , Microscopia de Fluorescência/métodos , Nitritos/química , Óxidos de Nitrogênio/metabolismo , Espectrometria de Fluorescência/métodos
7.
Analyst ; 144(14): 4371-4379, 2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31197299

RESUMO

SO2 has been recently identified as an essential gas messenger followed by NO, CO and H2S. However, abnormal concentrations of SO2 in our bodies can cause many diseases. Thus, the real-time monitoring of SO2 to well define the generation, physiological and pathological functions of SO2 is urgently needed. In this work, we developed a novel SO2 fluorescent probe on the basis of the conjugation of semi-cyanine and coumarin derivate dyes with superior features, such as near-infrared (NIR) and two-photon dual-mode monitoring, a large Stokes shift (175 nm), ultrafast response towards SO2 (within 10 s), high selectivity and photostability. Furthermore, this probe could sense SO2 by dual colorimetric and fluorescence means. In biological imaging, the probe was able to trace exogenous and endogenous SO2 in living cells, mitochondria, E. coli, zebrafish and mice under an NIR and two-photon dual-mode. These results demonstrated that the probe has strong potential for studying the physiological and pathological functions of SO2in vitro and in vivo.


Assuntos
Cumarínicos/química , Corantes Fluorescentes/química , Indóis/química , Dióxido de Enxofre/análise , Animais , Colorimetria/métodos , Cumarínicos/síntese química , Cumarínicos/efeitos da radiação , Cumarínicos/toxicidade , Escherichia coli , Feminino , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Corantes Fluorescentes/toxicidade , Células Hep G2 , Humanos , Indóis/síntese química , Indóis/efeitos da radiação , Indóis/toxicidade , Camundongos Endogâmicos BALB C , Microscopia de Fluorescência/métodos , Imagem Óptica/métodos , Fótons , Peixe-Zebra
8.
Methods Appl Fluoresc ; 7(3): 034004, 2019 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-31174198

RESUMO

In this work, we report a sensitive and selective turn-on fluorescence detection of cysteine with glutathione protected silver nanoclusters (GSH-Ag NCs). The glutathione stabilized silver nanoclusters were synthesized by the boiling water method. When excited at 380 nm, the GSH-Ag NCs exhibited a weak emission at about 680 nm, which could be enhanced by cysteine. The proposed method allows evaluation of cysteine in the range of 2-3000 µM with a detection limit of 0.51 µM. The recoveries were found to be 95.07%-101.38% when detecting cysteine contents in fetal bovine serum samples. In addition, we also discussed the possible mechanism for the fluorescence enhancement of GSH-Ag NCs by addition of cysteine. It might be the formation of cysteine and glutathione co-capped Ag NCs. This work reported a fluorimetric method for the assay of cysteine and provided a strategy for the synthesis of dual ligand-protected Ag nanoclusters.


Assuntos
Cisteína/sangue , Corantes Fluorescentes/química , Glutationa/química , Nanopartículas Metálicas/química , Prata/química , Animais , Bovinos , Cisteína/química , Fluorescência , Corantes Fluorescentes/efeitos da radiação , Limite de Detecção , Nanopartículas Metálicas/efeitos da radiação , Prata/efeitos da radiação , Espectrometria de Fluorescência/métodos , Raios Ultravioleta
9.
Analyst ; 144(11): 3546-3551, 2019 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-31041945

RESUMO

A turn-on two-photon fluorescent probe HCA-Green for hypochlorous acid (HOCl) was synthesized using 4-methylamino-1,8-naphthalimide (MNA) as a two-photon fluorophore and p-hydroxyaniline as a leaving-recognition domain. Both the probe and the fluorophore were investigated under one- and two-photon excitation modes. The fluorescence intensity of the probe was enhanced by ∼229-fold and ∼193-fold under one-photon and two-photon excitation, respectively, after reacting with HOCl. A maximal two-photon action cross-section of 50 GM was obtained under excitation at 810 nm. The probe exhibited high sensitivity with a detection limit of 42.3 nM, as well as high selectivity, low cytotoxicity, and good photostability. Two-photon microscopy (TPM) was conducted to visualize HOCl levels in living cells and tissues. The production of endogenous HOCl induced by lipopolysaccharide-mediated inflammation was successfully monitored with this probe.


Assuntos
Corantes Fluorescentes/química , Ácido Hipocloroso/análise , Inflamação/metabolismo , Animais , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Corantes Fluorescentes/toxicidade , Células HeLa , Humanos , Concentração de Íons de Hidrogênio , Inflamação/induzido quimicamente , Luz , Limite de Detecção , Lipopolissacarídeos , Camundongos Nus , Microscopia de Fluorescência/métodos , Ratos
10.
Analyst ; 144(12): 3807-3816, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31116194

RESUMO

Over the past decades, gallium (Ga) compounds have gained importance in the field of cancer treatment. Gallium acts as an iron mimic and disturbs iron-dependent propagation and other processes in tumor cells. However, the toxicity of gallium was also well documented in vitro and in vivo in animals. Though the oral administration of gallium in humans is less toxic, it has also been shown that a long period of administration could induce tumor fibrosis. Chromium (Cr), a naturally occurring heavy metal, is commonly used in industrial processes and can cause severe health problems in humans. It has been found to be closely involved in the metabolism of nucleic acids, proteins and fats in humans. Cr(iii) salts can be used as micronutrients and dietary supplements. However, similar to gallium (Ga3+), chromium (Cr3+) can build up to an excessive degree that is harmful to the human body. Therefore, it would be of great interest to develop chemosensing for the selective and sensitive detection of gallium and chromium ions in vitro and in vivo. Herein, we reported that an NBD-based (4-chloro-7-nitrobenzo-2-oxa-1,3-diazole) fluorescent probe (NBDT) was fabricated with demonstrated extraordinary specificity and sensitivity. A swift response toward Ga3+ and Cr3+ ions was discovered using fluorescence enhancement over a wide pH range and with cycle stability. Furthermore, lighted up by Ga3+ and Cr3+ ions in vitro, this NBDT sensor was successfully applied to detect exogenous Ga3+ and Cr3+ ions in MDA-MB-231 and HepG2 cells. Additionally, using zebrafish as the in vivo model, we demonstrated the capability of this NBDT for detecting and imaging Ga3+ and Cr3+ ions in zebrafish. Taken together, this NBDT has indicated great potential for detecting and monitoring Ga3+ and Cr3+ ions in vitro and in vivo.


Assuntos
4-Cloro-7-nitrobenzofurazano/análogos & derivados , Cromo/análise , Corantes Fluorescentes/química , Gálio/análise , 4-Cloro-7-nitrobenzofurazano/síntese química , 4-Cloro-7-nitrobenzofurazano/efeitos da radiação , Animais , Linhagem Celular Tumoral , Teoria da Densidade Funcional , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Humanos , Microscopia de Fluorescência/métodos , Modelos Químicos , Peixe-Zebra
11.
Talanta ; 201: 111-118, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31122400

RESUMO

Thiophenols as high toxic environmental pollutants are poisonous for animals and aquatic organisms. Therefore, it is indispensable to monitor thiophenols in the environment. Herein, a novel near-infrared fluorescent probe was developed for the detection of thiophenols, which was easily prepared by one-step coupling of 2,4-dinitrobenzenesulfonyl chloride with Nile blue. The probe showed a significant near infrared (∼675 nm) fluorescence "turn-on" response to thiophenols with some good features including chromogenic reaction, high sensitivity and selectivity, fast response, near-infrared emission along with low detection limit (1.8 nM). The probe was employed to rapidly and visually determine thiophenols in several industrial wastewaters with good recoveries (90-110%). Moreover, this probe has been demonstrated good capability for imaging thiophenol in HeLa cells.


Assuntos
Corantes Fluorescentes/química , Oxazinas/química , Fenóis/análise , Compostos de Sulfidrila/análise , Sulfonamidas/química , Águas Residuárias/análise , Poluentes Químicos da Água/análise , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Corantes Fluorescentes/toxicidade , Células HeLa , Humanos , Concentração de Íons de Hidrogênio , Raios Infravermelhos , Limite de Detecção , Microscopia Confocal/métodos , Modelos Químicos , Oxazinas/síntese química , Oxazinas/efeitos da radiação , Oxazinas/toxicidade , Espectrometria de Fluorescência/métodos , Sulfonamidas/síntese química , Sulfonamidas/efeitos da radiação , Sulfonamidas/toxicidade
12.
Talanta ; 201: 330-334, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31122431

RESUMO

A novel ratiometric fluorescence probe for hypochlorous acid was constructed by coumarin and pyridinium fluorophore based on the Forster resonance energy transfer (FRET) and intramolecular charge transfer (ICT) platform. In this ICT/FRET system, the energy transfer efficiency is high to 94.3%. Moreover, the probe could respond to hypochlorous acid with high selectivity and sensitivity, and exhibited a large Stokes shift. It was interesting to find that the probe could recognize hypochlorous acid via a new mechanism, in which the α-position of carbonyl group was oxidized to form a diketone derivative. More importantly, the probe was successfully applied to the ratiometric imaging of both exogenous and endogenous hypochlorous acid in living RAW 264.7 cells, with low toxicity and high photo-stability.


Assuntos
Cumarínicos/química , Corantes Fluorescentes/química , Ácido Hipocloroso/análise , Ácido Hipocloroso/metabolismo , Compostos de Piridínio/química , Animais , Cumarínicos/síntese química , Cumarínicos/efeitos da radiação , Desenho de Drogas , Fluorescência , Transferência Ressonante de Energia de Fluorescência/métodos , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Concentração de Íons de Hidrogênio , Luz , Limite de Detecção , Camundongos , Imagem Óptica/métodos , Compostos de Piridínio/síntese química , Compostos de Piridínio/efeitos da radiação , Células RAW 264.7
14.
Inorg Chem ; 58(13): 8379-8387, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31063357

RESUMO

Modulating the local environment around the emitting ions with component screening to increase the quantum yield and thermal stability is an effective and promising strategy for the design of high-performance fluorescence materials. In this work, smaller Lu3+ was introduced into the La3+ site in a Mn4+-activated LaAlO3 phosphor with the expectation of improving the luminescence properties via lattice contraction induced by cation substitution. Finally, a La1- xLu xAlO3:Mn4+ ( x = 0-0.04) perovskite phosphor with a high quantum yield of 86.0% and satisfactory thermal stability was achieved, and the emission peak at 729 nm well matches with the strongest absorption peak of the Phytochrome PFR. The favorable performances could be attributed to the suppressed cell volume and superior lattice rigidity after the substitution of Lu3+. This work not only obtains a highly efficient La1- xLu xAlO3:Mn4+ ( x = 0.02) phosphor, which holds great potential for application in plant-cultivation light-emitting diodes, but also provides an applicable strategy for further investigation of far-red-emitting phosphors.


Assuntos
Compostos de Alumínio/química , Equipamentos e Provisões Elétricas , Corantes Fluorescentes/química , Lantânio/química , Lutécio/química , Manganês/química , Agricultura/instrumentação , Compostos de Alumínio/síntese química , Compostos de Alumínio/efeitos da radiação , Cor , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Lantânio/efeitos da radiação , Lutécio/efeitos da radiação , Manganês/efeitos da radiação , Raios Ultravioleta
15.
Inorg Chem ; 58(13): 8587-8595, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31117633

RESUMO

A new N,O-based BODIPY ligand was synthesized and further utilized to develop highly fluorescent and photostable Ru(II), Rh(III), and Ir(III) metal complexes. The complexes were fully characterized by different analytical techniques including single-crystal XRD studies. The photostabilities and live cell imaging capabilities of the complexes were investigated via confocal microscopy. The complexes localized specifically in the mitochondria of live cells and showed negligible cytotoxicities at a concentration used for imaging purposes. They also exhibited high photostabilities, with fluorescence intensities remaining above 50% after 1800 scans.


Assuntos
Compostos de Boro/metabolismo , Complexos de Coordenação/metabolismo , Corantes Fluorescentes/metabolismo , Mitocôndrias/metabolismo , Transporte Biológico , Compostos de Boro/síntese química , Compostos de Boro/efeitos da radiação , Compostos de Boro/toxicidade , Complexos de Coordenação/síntese química , Complexos de Coordenação/efeitos da radiação , Complexos de Coordenação/toxicidade , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Corantes Fluorescentes/toxicidade , Células HeLa , Humanos , Irídio/química , Ligantes , Microscopia Confocal , Fotodegradação , Ródio/química , Rutênio/química
17.
Chem Commun (Camb) ; 55(36): 5279-5282, 2019 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-30993268

RESUMO

Under the synergistic effects of protonation and deprotonation, a light-activated fluorescent probe (UV-SP) exhibited "cycle-reversible intramolecular charge transfer (ICT)" for different pH after activation by UV light, resulting in emission of multiple ratio fluorescent signals (FI563/FI595 and FI664/FI595). Based on these kinds of response signals, UV-SP can specifically monitor the cycle-reversible trace change of intracellular pH caused by UV radiation. More importantly, according to the stable and invariant multiple ratio fluorescent signals, UV-SP can sort cells entering programmed death.


Assuntos
Apoptose/fisiologia , Benzopiranos/química , Corantes Fluorescentes/química , Compostos de Espiro/química , Animais , Benzopiranos/síntese química , Benzopiranos/efeitos da radiação , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Células Hep G2 , Humanos , Concentração de Íons de Hidrogênio , Camundongos , Microscopia Confocal/métodos , Células NIH 3T3 , Sensibilidade e Especificidade , Compostos de Espiro/síntese química , Compostos de Espiro/efeitos da radiação , Raios Ultravioleta
18.
Analyst ; 144(10): 3381-3388, 2019 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-30984924

RESUMO

Hydrogen sulfide (H2S) exists in the cytosol and mitochondria of mammalian cells as a signaling molecule. Scientists have explored many important physiological functions of H2S, such as regulating vasodilator relaxation, protecting living cells and avoiding damage. The measurement of H2S is therefore necessary for exploring the biological function of cells and tissues. Herein, we report the design and synthesis of a new aggregation-induced emission luminogen (AIEgen) with greater conjugation and more positive charges, based on previous research on mitochondrial-targeted luminogens. The Indo-TPE-Indo can enter cells rapidly, as compared with an AIEgen with only one indolium (TPE-indo), and can selectively recognize HS- in mitochondria with the nucleophilic reaction of indolium and HS-. The linear range (1-100 µM) of HS- sensing can satisfy the requirement for HS- concentration in living cells and tumors.


Assuntos
Corantes Fluorescentes/química , Sulfeto de Hidrogênio/análise , Indóis/química , Mitocôndrias/metabolismo , Estilbenos/química , Animais , Estabilidade de Medicamentos , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Células Endoteliais da Veia Umbilical Humana , Humanos , Sulfeto de Hidrogênio/metabolismo , Indóis/síntese química , Indóis/efeitos da radiação , Luz , Células MCF-7 , Camundongos , Microscopia Confocal/métodos , Microscopia de Fluorescência/métodos , Neoplasias/metabolismo , Estilbenos/síntese química , Estilbenos/efeitos da radiação
19.
Analyst ; 144(10): 3422-3427, 2019 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-31011741

RESUMO

Mitochondria as essential organelles play critical roles in cellular metabolism. Mitochondrial pH is a vital parameter that directly affects the unique function of mitochondria. Herein, we present a new ratiometric fluorescent probe M-pH for monitoring the pH within the mitochondria. M-pH consists of a stable and large π-electron conjugated merocyanine system. The lipophilic cationic benzyl group will facilitate the accumulation of M-pH in mitochondria. The phenol unit is the recognition moiety, achieving the ratiometric sensing of pH changes. The experimental results indicate that M-pH displays ratiometric fluorescence response to different pH values. Meanwhile, M-pH shows negligible response to common species, and has high stability and low cytotoxicity. In biological experiments, M-pH can solely accumulate in mitochondria and visualize the pH changes during mitophagy and cell apoptosis. We thus believe that M-pH has great potential as a practical tool for real-time monitoring of pH changes of mitochondria, contributing to revealing the pathogenesis of mitochondrial pH associated diseases.


Assuntos
Corantes Fluorescentes/química , Indóis/química , Mitocôndrias/metabolismo , Fenóis/química , Apoptose/fisiologia , Linhagem Celular Tumoral , Colorimetria/métodos , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Corantes Fluorescentes/toxicidade , Humanos , Concentração de Íons de Hidrogênio , Indóis/síntese química , Indóis/efeitos da radiação , Indóis/toxicidade , Luz , Microscopia Confocal/métodos , Microscopia de Fluorescência/métodos , Degradação Mitocondrial/fisiologia , Fenóis/síntese química , Fenóis/efeitos da radiação , Fenóis/toxicidade
20.
Nat Commun ; 10(1): 1232, 2019 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-30874551

RESUMO

Photoswitchable molecules have multiple applications in the physical and life sciences because their properties can be modulated with light. Fluxional molecules, which undergo rapid degenerate rearrangements in the electronic ground state, also exhibit switching behavior. The stochastic nature of fluxional switching, however, has hampered its application in the development of functional molecules and materials. Here we combine photoswitching and fluxionality to develop a fluorophore that enables very long (>30 min) time-lapse single-molecule localization microscopy in living cells with minimal phototoxicity and no apparent photobleaching. These long time-lapse experiments allow us to track intracellular organelles with unprecedented spatiotemporal resolution, revealing new information of the three-dimensional compartmentalization of synaptic vesicle trafficking in live human neurons.


Assuntos
Corantes Fluorescentes/química , Microscopia Intravital/métodos , Sondas Moleculares/química , Neurônios/química , Vesículas Sinápticas/química , Corantes Fluorescentes/efeitos da radiação , Células HeLa , Humanos , Isomerismo , Luz , Microscopia de Fluorescência/métodos , Sondas Moleculares/efeitos da radiação , Neurônios/citologia , Neurônios/metabolismo , Fotodegradação , Imagem Individual de Molécula/métodos , Espectrometria de Fluorescência , Vesículas Sinápticas/metabolismo , Imagem com Lapso de Tempo/métodos
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