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1.
Food Chem ; 298: 125014, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260973

RESUMO

Natural red purple dye, Betanin, was extracted from the beetroot, purified by aqueous two- phase extraction and gel permeation column chromatography, and used as a reducing agent for the synthesis of silver-betanin core-shell triangular nanodisks for the first time. Spectroscopic data show that the nanoparticle structure is core@shell like with Ag as core and betanin as shell. Langmuir monolayer model (qm = 32.4 mg/g, RL = 0.99 and R2 = 0.997) was the best fit adsorption isotherm for the dye removal. Adsorption kinetics is well explained by pseudo-second-order equation. Gibbs free energy (ΔG0 = -2.59 kJ mol-1), enthalpy (ΔH0 = 18.05 kJ mol-1), and entropy (ΔS0 = 68.92 J/K/mol) were calculated and discussed. The antibacterial activity of betanin-AgNPs were determined against Escherichia coli MTCC-450 (E. coli) and Staphylococcus aureus MTCC-3160 (S. aureus) bacteria.


Assuntos
Antibacterianos/farmacologia , Beta vulgaris/química , Betacianinas/farmacologia , Corantes/farmacologia , Nanopartículas Metálicas/química , Prata/química , Adsorção , Antibacterianos/química , Betacianinas/química , Betacianinas/isolamento & purificação , Corantes/química , Corantes/isolamento & purificação , Entropia , Escherichia coli/efeitos dos fármacos , Cinética , Staphylococcus aureus/efeitos dos fármacos , Termodinâmica
2.
Photochem Photobiol Sci ; 18(8): 2003-2011, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31268087

RESUMO

Here we report the activatable photosensitizer BromoAcroB, a brominated BODIPY dye incorporating a reactive acrolein warhead. The acrolein moiety serves as an intramolecular switch, deactivating the BODIPY dye in its singlet and triplet excited states via internal conversion. Thiolate addition to this moiety disables the intramolecular quenching mechanism restoring the photosensitizing properties of the parent dye, characterized by a quantum yield of singlet oxygen photosensitization of 0.69 ± 0.02. In cell cultures, and upon thiol adduct formation, BromoAcroB induced light-dependent cell death in MRC-5 and HeLa cell lines. Using fluorescence microscopy and upon measuring the low yet non-negligible emission of the activated compound, we show that the phototoxicity of the dormant photosensitizer correlated with the quantity of BromoAcroB adducts generated. BromoAcroB thus serves as a dormant photosensitizer sensitive to intracellular electrophile response. Our results highlight the effective control of a triplet state process by modulation of an unsaturated moiety on the BODIPY scaffold and underscore the mechanistic opportunities arising for controlled singlet oxygen production in cells specifically sensitive to electrophile stress.


Assuntos
Acroleína/farmacologia , Compostos de Boro/farmacologia , Corantes/farmacologia , Cisteína/farmacologia , Fármacos Fotossensibilizantes/farmacologia , Oxigênio Singlete/farmacologia , Acroleína/química , Compostos de Boro/química , Morte Celular/efeitos dos fármacos , Corantes/síntese química , Corantes/química , Cisteína/química , Células HeLa , Humanos , Luz , Microscopia de Fluorescência , Estrutura Molecular , Fármacos Fotossensibilizantes/síntese química , Fármacos Fotossensibilizantes/química , Teoria Quântica , Oxigênio Singlete/química
3.
Photochem Photobiol Sci ; 18(8): 2012-2022, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31282525

RESUMO

Organic-metal complexes are promising molecules for use in photodynamic therapy (PDT). The aim of this study was to investigate in vitro effects of novel Ru(ii) and Ir(iii) BODIPY complexes for PDT. These hybrid organic-metal molecules (Ru-BD and Ir-BD) have been synthesized via reactions of a BODIPY precursor (BD) with a phenanthroline unit bearing Ru(ii) (3) and novel Ir(iii) (4) compounds. The crystal structures of the new distyryl BODIPY (BD) and Ru(ii) complex (3) are also reported. The photophysical and singlet oxygen generation properties of Ru-BD and Ir-BD were investigated in comparison with unsubstituted BODIPY (BD). Moreover, Ru-BD and Ir-BD have been biologically evaluated in vitro in chronic myeloid leukemia and cervical cancer cell lines in terms of photodynamic therapy efficacy in the presence of BD control. These complexes were not toxic in the dark but red light was needed to induce cell death. These data support the fact that Ru-BD could be accepted as a valuable photosensitizer-drug for further PDT treatment.


Assuntos
Antineoplásicos/farmacologia , Compostos de Boro/farmacologia , Corantes/farmacologia , Compostos Organometálicos/farmacologia , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Antineoplásicos/síntese química , Antineoplásicos/química , Compostos de Boro/síntese química , Compostos de Boro/química , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Corantes/síntese química , Corantes/química , Cristalografia por Raios X , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Irídio/química , Irídio/farmacologia , Modelos Moleculares , Estrutura Molecular , Compostos Organometálicos/síntese química , Compostos Organometálicos/química , Fármacos Fotossensibilizantes/síntese química , Fármacos Fotossensibilizantes/química , Rutênio/química , Rutênio/farmacologia , Oxigênio Singlete/análise , Oxigênio Singlete/metabolismo , Células Tumorais Cultivadas
4.
Ann Thorac Surg ; 108(2): e141-e143, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30951697

RESUMO

Intravenous indocyanine green injection is useful for the identification of the intersegmental border by infrared thoracoscopy during anatomic segmentectomy. However, surgeons encounter cases in which visualization of the intersegmental border is difficult. In particular, intravenous indocyanine green fluorescence in the upper lobe is occasionally obscured by to the relatively lesser blood flow in the upper lobe pulmonary arteries. This report describes an interlobar pulmonary artery compression method that is a simple and effective technique for clearly visualizing the intersegmental border through infrared thoracoscopy with intravenous indocyanine green during upper lobe segmentectomy.


Assuntos
Verde de Indocianina/farmacologia , Neoplasias Pulmonares/cirurgia , Pneumonectomia/métodos , Artéria Pulmonar/cirurgia , Toracoscopia/métodos , Idoso , Corantes/farmacologia , Feminino , Humanos , Neoplasias Pulmonares/diagnóstico , Masculino
5.
Nano Lett ; 19(5): 3229-3237, 2019 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-30957499

RESUMO

To develop a highly efficient strategy against tumors, here, a nanocombination (PDC/P@HCuS) was designed and constructed to actualize chemo-phototherapy with the assistance of fluorescence (FL) and photoacoustic (PA) images. First, a type of organic-inorganic hybrid nanosystem (P@HCuS) was engineered by coupling the fluorescence-labeled amphiphilic fPEDC copolymer on the surface of hollow mesoporous copper sulfide nanoparticle (HCuS), in which HCuS was used as a photothermal and PA agent; fPEDC as a stabilizer, chromophore, and redox/pH-sensitive gatekeeper; and both of them as drug carriers. Then, a peptide-drug conjugate (cRGD-SMCC-DM1, PDC), as a molecular targeted maytansinoid, was loaded inside of P@HCuS to form PDC/P@HCuS. Next, the PDC/P@HCuS was investigated carefully with or without near-infrared (NIR) laser irradiation. In vitro, the nanocombination exhibited stimuli-responsive drug release, obvious cellular uptake, strong cytotoxicity to tumor cells, significant impact on cell cycle, and cytoskeleton and cellular proteomics as well as evident permeability into the tumor sphere, most of which could be boosted by NIR laser irradiation. In vivo, the nanocombinaiton exerted good FL/PA imaging features and photothermal efficiency, achieved the best antitumor efficacy in the presence of NIR laser irradiation, and showed excellent biosafety. Together, it demonstrated that the PDC/P@HCuS, representing a chemo-phototherapy based on a nanocombination associated with peptide-drug conjugate, could achieve the highly efficient antitumor effect.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Peptídeos/farmacologia , Técnicas Fotoacústicas , Antineoplásicos/síntese química , Antineoplásicos/química , Antineoplásicos/farmacologia , Neoplasias da Mama/patologia , Corantes/química , Corantes/farmacologia , Cobre/química , Cobre/farmacologia , Doxorrubicina/química , Doxorrubicina/farmacologia , Liberação Controlada de Fármacos , Feminino , Humanos , Hipertermia Induzida , Células MCF-7 , Maitansina/química , Maitansina/farmacologia , Nanopartículas , Peptídeos/química , Fototerapia , Sulfetos/química , Sulfetos/farmacologia
6.
Parasitol Int ; 71: 177-179, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31004805

RESUMO

Giardia cysts stained with hot carbolfuchsin were used as internal controls in a concentration method for surface water samples. The morphological integrity of stained cysts and the stain's stability and intensity were tested with each of the chemical reagents used in the aluminum sulfate flocculation method. No alterations in morphology or color were noted. The stained cyst preparation has a low cost, high stability, and suitability for both light and immunofluorescent microscopy, making it affordable to researchers in low- and middle-income countries.


Assuntos
Corantes/farmacologia , Água Doce/parasitologia , Giardia/isolamento & purificação , Oocistos/isolamento & purificação , Compostos de Alúmen , Fezes/parasitologia , Floculação , Giardia/efeitos dos fármacos , Microscopia de Fluorescência , Oocistos/efeitos dos fármacos , Parasitologia/economia , Parasitologia/métodos , Corantes de Rosanilina/farmacologia , Coloração e Rotulagem/economia , Coloração e Rotulagem/métodos
7.
ACS Appl Mater Interfaces ; 11(14): 13079-13090, 2019 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-30844230

RESUMO

Nanoemulsions (NEs) are biocompatible lipid nanoparticles composed of an oily core stabilized by a surfactant shell. It is acknowledged that the surface decoration with poly(ethylene glycol), through the use of nonionic surfactants, confers high stealth in biological medium with reduced nonspecific interactions. Tracking individual NE by fluorescence microscopy techniques would lead to a better understanding of their behavior in cells and thus require the development of bright single particles with enhanced photostability. However, the understanding of the relationship between the physicochemical properties and chemical composition of the NEs, on the one hand, and its fluorescence properties of encapsulated dyes, on the other hand, remains limited. Herein, we synthesized three new dioxaborine barbituryl styryl (DBS) dyes that displayed high molar extinction coefficients (up to 120 000 M-1 cm-1) with relatively low quantum yields in solvents and impressive fluorescence enhancement when dissolved in viscous oils (up to 0.98). The reported screening of nine different oils allowed disclosing a range of efficient "oil/dye" couples and understanding the main parameters that lead to the brightest NEs. We determine vitamin E acetate/DBS-C8 as the representative most efficient couple, combining high dye loading capabilities and low aggregation-induced quenching, leading to <50 nm ultrabright NEs (with brightness as high as 30 × 106 M-1 cm-1) with negligible dye leakage in biological media. Beyond a comprehensive optical and physicochemical characterization of fluorescent NEs, cellular two-photon excitation imaging was performed with polymer-coated cell penetrating NEs. Thanks to their impressive brightness and photostability, NEs displaying different charge surfaces were microinjected in HeLa cells and were individually tracked in the cytosol to study their relative velocity.


Assuntos
Rastreamento de Células , Emulsões/farmacologia , Nanopartículas/química , Polímeros/farmacologia , Corantes/química , Corantes/farmacologia , Emulsões/química , Fluorescência , Células HeLa , Humanos , Lipídeos/química , Nanopartículas/administração & dosagem , Polietilenoglicóis/química , Polietilenoglicóis/farmacologia , Polímeros/química
8.
Medicine (Baltimore) ; 98(11): e14831, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30882669

RESUMO

Thoracoscopic resection of small subsolid nodules is challenging and requires preoperative localization. We investigated the efficacy, safety, and factors affecting accuracy in localizing pulmonary nodules with electromagnetic navigation bronchoscopy (ENB)-guided dye marking.Patients with small subsolid nodule(s) who underwent thoracoscopic resection after ENB-guided dye marking were retrospectively reviewed. Dye marking was performed at the nearest pleura and the localized nodule(s) was resected thoracoscopically. Efficacy was evaluated by success rates of dye marking and resection of nodules. Navigation accuracy was represented by target distance, which was the closest distance between target and the tip of locatable guide. Factors affecting target distance were evaluated by linear regression analyses.Twenty-nine ENB-guided dye markings were done for 24 nodules in 20 patients. The success rate of the dye marking and nodule localization were 93.1% (27/29) and 95.8% (23/24), respectively. Twenty-three nodules were completely resected thoracoscopically without conversion. There were no ENB-related complications: pneumothorax or bronchopulmonary hemorrhage. Nine targets were in the upper, 14 in the middle, and 6 in the lower zone. Even though navigation time was longer in the upper zone, target distance showed no significant inter-zone difference. Approach angle was the only significant predictor for target distance (0-45°, estimate = -1.24, P = .01; 45-90°, estimate = -1.26, P = .006; reference = ≥90°).Localization with ENB-guided dye marking is effective and safe for thoracoscopic resection of small subsolid nodules. For better performance, a pathway with smaller approach angle (<90°) should be selected to increase the navigation accuracy.


Assuntos
Broncoscopia/métodos , Neoplasias Pulmonares , Nódulos Pulmonares Múltiplos , Coloração e Rotulagem/métodos , Cirurgia Assistida por Computador/métodos , Cirurgia Torácica Vídeoassistida/métodos , Corantes/farmacologia , Feminino , Humanos , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/cirurgia , Masculino , Pessoa de Meia-Idade , Nódulos Pulmonares Múltiplos/patologia , Nódulos Pulmonares Múltiplos/cirurgia , República da Coreia , Estudos Retrospectivos , Resultado do Tratamento , Carga Tumoral
9.
Jpn J Ophthalmol ; 63(3): 243-254, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30806869

RESUMO

PURPOSE: To evaluate the efficacy of indocyanine-green angiography (ICGA)-guided navigated focal laser photocoagulation for diabetic macular edema (DME). STUDY DESIGN: Prospective, interventional case series. METHODS: Six patients (8 eyes) were enrolled in this study. Fluorescein angiography (FA) and ICGA were performed using the Heidelberg Retina Angiogram 2 (Heidelberg Engineering). Navigated focal laser photocoagulation was delivered to the microaneurysms on ICGA using Navilas® (OD-OS GmbH, Germany). Central retinal thickness (CRT) and macular volume (MV) were measured by Cirrus HD-OCT (Carl Zeiss Meditec). At 6 months, the best-corrected visual acuity (BCVA), CRT and MV were compared to the values measured on day 0. The distances from the center of fovea to the closest microaneurysms (MAs) were measured on the pre-planned Navilas® image. RESULTS: All eyes had previous treatment history. At 6 months, ICGA-guided navigated focal laser photocoagulation significantly reduced the CRT and the MV (p<0.05), and there was improvement in the BCVA (p<0.05). At 3 months, 5 out of the 8 eyes (63%) underwent additional ICGA-guided navigated focal laser photocoagulation due to remnants of MAs that had been confirmed by ICGA. There was no observed recurrence of edema after the ICGA-guided navigated focal laser photocoagulation during the 6-month follow-up. The mean distance from the center of fovea to the closest MAs was 624.8 ± 377.7 µm (range 336.0-1438.9 µm). CONCLUSION: Our data suggest ICGA-guided navigated focal laser photocoagulation may be effective for the treatment of DME.


Assuntos
Retinopatia Diabética/cirurgia , Angiofluoresceinografia/métodos , Verde de Indocianina/farmacologia , Terapia a Laser/métodos , Macula Lutea/patologia , Edema Macular/cirurgia , Cirurgia Assistida por Computador/métodos , Idoso , Corantes/farmacologia , Retinopatia Diabética/complicações , Retinopatia Diabética/diagnóstico , Feminino , Seguimentos , Fundo de Olho , Humanos , Edema Macular/diagnóstico , Edema Macular/etiologia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Tomografia de Coerência Óptica , Resultado do Tratamento , Acuidade Visual
10.
Dis Colon Rectum ; 62(4): 422-428, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30730457

RESUMO

BACKGROUND: Correctly predicting the depth of tumor invasion in the colorectal wall is crucial for successful endoscopic resection of superficial colorectal neoplasms. OBJECTIVE: The aim of this study was to assess the accuracy of magnifying chromoendoscopy in a Western medical center to predict the depth of invasion by the pit pattern classification in patients with colorectal neoplasms with a high risk of submucosal invasion. DESIGN: This single-center retrospective study, from a prospectively collected database, was conducted between April 2009 and June 2015. SETTINGS: The study was conducted at a single academic center. PATIENTS: Consecutive patients with colorectal neoplasms with high risk of submucosal invasion were included. These tumors were defined by large (≥20 mm) sessile polyps (nonpedunculated), laterally spreading tumors, or depressed lesions of any size. INTERVENTIONS: Patients underwent magnifying chromoendoscopy and were classified according to the Kudo pit pattern. The therapeutic decision, endoscopic or surgery, was defined by the magnification assessment. MAIN OUTCOME MEASURES: Sensitivity, specificity, and positive and negative predictive values of magnifying chromoendoscopy for assessment of these lesions were determined. RESULTS: A total of 123 lesions were included, with a mean size of 54.0 ± 37.1 mm. Preoperative magnifying chromoendoscopy with pit pattern classification had 73.3% sensitivity, 100% specificity, 100% positive predictive value, 96.4% negative predictive value, and 96.7% accuracy to predict depth of invasion and consequently to guide the appropriate treatment. Thirty-three rectal lesions were also examined by MRI, and 31 were diagnosed as T2 lesions. Twenty two (70.1%) of these lesions were diagnosed as noninvasive by magnifying colonoscopy, were treated by endoscopic resection, and met the curative criteria. LIMITATIONS: This was a single-center retrospective study with a single expert endoscopist experience. CONCLUSIONS: Magnifying chromoendoscopy is highly accurate for assessing colorectal neoplasms suspicious for submucosal invasion and can help to select the most appropriate treatment. See Video Abstract at http://links.lww.com/DCR/A920.


Assuntos
Colonoscopia/métodos , Neoplasias Colorretais , Corantes/farmacologia , Ressecção Endoscópica de Mucosa , Aumento da Imagem/métodos , Mucosa Intestinal , Idoso , Brasil , Neoplasias Colorretais/patologia , Neoplasias Colorretais/cirurgia , Ressecção Endoscópica de Mucosa/efeitos adversos , Ressecção Endoscópica de Mucosa/métodos , Feminino , Humanos , Mucosa Intestinal/diagnóstico por imagem , Mucosa Intestinal/patologia , Masculino , Invasividade Neoplásica , Estadiamento de Neoplasias , Valor Preditivo dos Testes , Prognóstico , Estudos Retrospectivos , Sensibilidade e Especificidade
11.
Dis Colon Rectum ; 62(4): 470-475, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30640315

RESUMO

BACKGROUND: Almost no prospective data on endoscopy in MUTYH monoallelic carriers are available. OBJECTIVE: This study aimed to define the prevalence of colorectal and duodenal adenomas in a population of people presenting with a single mutation of the MUTYH gene and being first-degree relatives of biallelic MUTYH mutation carriers. DESIGN: This study is a prospective cohort evaluation. PATIENTS: Patients were first-degree relatives of a patient who had polyposis with biallelic MUTYH mutation and carrying a single gene mutation of the gene from 12 French centers. SETTINGS: This is a multicenter study. INTERVENTION: Detailed data on life habits (tobacco, alcohol, and nonsteroidal anti-inflammatory drugs), extraintestinal manifestations, and germline analysis were recorded. Complete endoscopic evaluation (colonoscopy and upper endoscopy) with chromoendoscopy was performed. RESULTS: Sixty-two patients were prospectively included (34 women (55%), mean age of 54, range 30-70 years). Thirty-two patients (52%) presented with colorectal polyps at colonoscopy. Of these patients with polyps, 15 (25%) had only adenomas, 8 (13%) had only hyperplastic polyps, 1 (1%) had sessile serrated adenomas, and 8 (13%) had adenomas and/or sessile serrated adenomas. We detected, in total, 29 adenomas with low-grade dysplasia, 5 adenomas with high-grade dysplasia, and 6 sessile serrated adenomas. Fourteen patients (23%) presented with a single adenoma, and 10 (16%) had 1 to 5 adenomas. No patient had more than 5 adenomas. At upper endoscopy, 3 had a limited number of fundic gland polyps; none had duodenal adenomas. The 2 main missense mutations c.1145G>A, p.Gly382Asp and c.494A>G, p.Tyr165Cys were associated with the development of colorectal adenomas/serrated polyps in these monoallelic carriers. LIMITATIONS: This study was limited by the small number of patients. CONCLUSIONS: This prospective study provides unique prospective data suggesting that monoallelic mutation carriers related to patients with polyposis show no colorectal polyposis and have very limited upper GI manifestations justifying an endoscopic follow-up. See Video Abstract at http://links.lww.com/DCR/A862.


Assuntos
Adenoma , Polipose Adenomatosa do Colo , Neoplasias Colorretais , DNA Glicosilases/genética , Neoplasias Duodenais , Endoscopia do Sistema Digestório/métodos , Adenoma/genética , Adenoma/patologia , Polipose Adenomatosa do Colo/diagnóstico , Polipose Adenomatosa do Colo/epidemiologia , Polipose Adenomatosa do Colo/genética , Adulto , Idoso , Estudos de Coortes , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Corantes/farmacologia , Neoplasias Duodenais/genética , Neoplasias Duodenais/patologia , Saúde da Família , Feminino , França/epidemiologia , Humanos , Aumento da Imagem/métodos , Masculino , Pessoa de Meia-Idade , Mutação , Avaliação de Resultados (Cuidados de Saúde) , Estudos Prospectivos
12.
Graefes Arch Clin Exp Ophthalmol ; 257(3): 517-528, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30607553

RESUMO

PURPOSE: To test the applicability of the acai dye at a 25% concentration for identifying the posterior hyaloids and internal limiting membranes (ILMs) during pars plana vitrectomy (PPV) in human eyes with macular holes (MHs). METHODS: This study included 25 patients with chronic idiopathic MHs. The exclusion criteria included glaucoma, previous significant ocular conditions, and previous ocular surgeries except uncomplicated cataract. Ten surgeons performed 23-gauge four-port PPV, phacoemulsification, posterior hyaloid detachment, ILM peeling guided by dye staining, and perfluoropropane injection. The patients remained prone for 5 days postoperatively. The patients were evaluated postoperatively after 1, 30, and 180 days. The surgeons completed a questionnaire regarding the dye's staining abilities. RESULTS: The posterior hyaloids and ILMs stained purple in all eyes. The final best-corrected visual acuity improved significantly (p < 0.001) from preoperatively (1.37 ± 0.29) to 180 days postoperatively (1.05 ± 0.43). The MHs closed in 76% of eyes. CONCLUSION: The acai dye at a 25% concentration identified posterior hyaloids and ILMs during PPVs in humans. Dye toxicity was unlikely.


Assuntos
Antocianinas/farmacologia , Euterpe , Angiofluoresceinografia/métodos , Perfurações Retinianas/diagnóstico , Tomografia de Coerência Óptica/métodos , Vitrectomia/métodos , Corpo Vítreo/diagnóstico por imagem , Idoso , Doença Crônica , Corantes/farmacologia , Feminino , Seguimentos , Fundo de Olho , Humanos , Período Intraoperatório , Masculino , Perfurações Retinianas/cirurgia , Coloração e Rotulagem/métodos , Fatores de Tempo , Corpo Vítreo/cirurgia
13.
Cornea ; 38(1): 54-61, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30222713

RESUMO

PURPOSE: To report the use of different superficial keratopigmentation (KTP) techniques for restoring or enhancing cosmetic appearance of eyes impaired for several reasons. METHODS: This is a retrospective, consecutive, noncomparative interventional series of cases, in which 136 cosmetically disabled eyes (due to different corneal abnormalities) of 130 patients were included. A total of 222 procedures of superficial manual KTP and superficial automated KTP were performed. The procedures were divided into 2 groups: first and second-stage procedures. The patients' cosmetic appearance and satisfaction were evaluated and graded as excellent, good, or poor. RESULTS: A total of 222 procedures were performed, 57.2% were superficial automated KTP, 22.5% were superficial manual KTP, and 20.3% included the combination of superficial KTP with other KTP techniques. Six procedures were purely cosmetic, 16 therapeutic functional, and 200 therapeutic cosmetic. Superficial KTP, as a second-stage procedure, was performed in 13 eyes that were previously pigmented by intrastromal techniques. Superficial KTP as an initial indication (first-stage procedure) alone or in combination with another KTP technique in the same surgery was performed in 123 eyes, in which 44.7% of them were reoperated once or more. Best-corrected visual acuity changed insignificantly, and the intraocular pressure decreased significantly in all the studied groups. No intraoperative complications were observed, 11.2% of eyes with a follow-up time of more than 3 months developed postoperative complications. Most of the patients (98.5%) were satisfied. CONCLUSIONS: The modern superficial KTP procedure provides the possibility to improve the cosmetic appearance of impaired eyes, as an alternative to evisceration, enucleation, prosthetic contact lenses, or keratoplasty.


Assuntos
Corantes/farmacologia , Córnea/cirurgia , Opacidade da Córnea/cirurgia , Previsões , Satisfação do Paciente , Tatuagem/métodos , Acuidade Visual , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Seguimentos , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
15.
J Laparoendosc Adv Surg Tech A ; 29(4): 476-483, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30589374

RESUMO

BACKGROUND: Anastomotic leakage is a severe complication after gastric cancer surgery. Inadequate blood supply is regarded as an important risk factor. The aim of the study was to evaluate the feasibility and usefulness of intraoperative assessment of anastomotic vascular perfusion in gastric cancer surgery using near-infrared (NIR) camera imaging with indocyanine green (ICG)-enhanced fluorescence technique. MATERIALS AND METHODS: From March 2015 to 2016, 30 patients undergoing laparoscopic gastrectomy for gastric cancer were prospectively evaluated. After completing the anastomosis, 2.5-5.0 mg of ICG was injected via peripheral veins. All anastomoses and resection margins were investigated using NIR camera to assess anastomotic perfusion. The assessment was performed using the adopted perfusion score of fluorescence activity, which ranged from 1 to 5 (1 = no uptake, and 5 = iso-fluorescent to all other segments). RESULTS: Twenty-six distal gastrectomy (20 gastroduodenostomies, 6 gastrojejunostomies), 3 total gastrectomies (TG), and 1 pylorus-preserving gastrectomy were performed. The gap of visualization was 4.1 ± 3.2 minutes (range, 2-15) after ICG injection. Twenty-three of 30 patients (76.7%) showed technically successful ICG visualization. Among gastroduodenostomies, the average scores for gastric and duodenal sides were 3.5 and 3.7. Among gastrojejunostomies, the average scores for gastric, jejunal, and duodenal stump sides were 3.5, 4.0, and 3.8 (jejunojejunostomy, 3.5). Among TG, the average scores for esophagojejunostomy, duodenal stump, and jejunojejunostomy were 3.7, 4.0, 4.0, and 4.7. One case of leakage occurred in this study. Other complications included fluid collection and stenosis in 1 patient each. CONCLUSIONS: This study showed intraoperative ICG angiography using NIR camera is feasible and provides imaging of anastomotic blood flow. Further studies are needed for practice.


Assuntos
Gastrectomia/métodos , Verde de Indocianina/farmacologia , Intestinos/irrigação sanguínea , Laparoscopia/métodos , Artérias Mesentéricas/fisiopatologia , Imagem Óptica/métodos , Imagem de Perfusão/métodos , Anastomose Cirúrgica/métodos , Fístula Anastomótica/prevenção & controle , Corantes/farmacologia , Feminino , Fluorescência , Humanos , Masculino , Artérias Mesentéricas/diagnóstico por imagem , Pessoa de Meia-Idade , Monitorização Intraoperatória/métodos , Estudos Prospectivos , Fatores de Risco , Circulação Esplâncnica/fisiologia
16.
Int J Nanomedicine ; 13: 8025-8035, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30568443

RESUMO

Background: Carbon quantum dots (CDots) have recently been reported as a new class of visible light activated antimicrobial nanomaterials. This study reports the synergistic photoactivated antimicrobial interactions of CDots with photosensitizers on bacterial cells. Methods: The antimicrobial effects of the CDots with surface passivation molecules 2,2'-(ethylenedioxy)bis(ethylamine) in combination with photosensitizer methylene blue (MB) or toluidine blue (TB) at various concentrations were evaluated against Escherichia coli cells with and without 1-hour visible light illumination. The broth microdilution checkerboard method and isobologram analysis were used for determining if synergistic effect existed between CDots and MB or TB. Results: The results showed that CDots alone at a concentration of 5 µg/mL did not display antimicrobial effects, 1 µg/mL MB alone only decreased 1.86 log of viable cell numbers, but the combination treatment with 5 µg/mL CDots combined with 1 µg/mL MB completely inhibited bacteria growth, resulted in 6.2 log viable cell number reduction, suggesting synergistic interaction between the two. The antimicrobial effects of CDots/TB combination exhibited similarly synergistic effects on E. coli cells. These synergistic effects between CDots and MB or TB were further confirmed using the checkerboard microdilution methods, where the fractional inhibitory concentration index value (0.5) and the isobologram analyses. The synergistic interactions were also correlated to the increased generation of intracellular reactive oxygen species in E. coli cells upon the combination treatments of CDots/MB or CDots/TB. Conclusion: The study demonstrated the synergistic photoactivated antimicrobial effects of CDots in combination with other photosensitizers. Such synergistic effect may open new strategies for developing highly effective antimicrobial methods.


Assuntos
Anti-Infecciosos/farmacologia , Carbono/farmacologia , Corantes/farmacologia , Escherichia coli/efeitos dos fármacos , Fármacos Fotossensibilizantes/farmacologia , Pontos Quânticos/química , Antibacterianos/farmacologia , Escherichia coli/ultraestrutura , Fluorescência , Azul de Metileno/farmacologia , Testes de Sensibilidade Microbiana , Viabilidade Microbiana/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Cloreto de Tolônio/farmacologia
17.
Chemosphere ; 210: 968-976, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30208557

RESUMO

In situ phytoremediation of dyes from textile wastewater was carried out in a high rate transpiration system ridges (91.4 m × 1.0 m) cultivated independently with Tagetes patula, Aster amellus, Portulaca grandiflora and Gaillardia grandiflora which reduced American Dye Manufacturers Institute color value by 59, 50, 46 and 73%, respectively within 30 d compared to dye accumulated in unplanted ridges. Significant increase in microbial count and electric conductivity of soil was observed during phytoremediation. Reduction in the contents of macro (N, P, K and C), micro (B, Cu, Fe and Mn) elements and heavy metals (Cd, As, Pb and Cr) was observed in the soil from planted ridges due to phyto-treatment. Root tissues of these plants showed significant increase in the specific activities of oxido-reductive enzymes such as lignin peroxidase, laccase, veratryl alcohol oxidase, tyrosinase and azo reductase during decolorization of textile dyes from soil. Anatomical studies of plants roots revealed the occurrence of textile dyes in tissues and subsequent degradation. A minor decrease in plant growth was also observed. Overall surveillance suggests that the use of garden ornamental plants on the ridges of constructed wetland for the treatment of dyes from wastewater along with the consortia of soil microbial flora is a wise and aesthetically pleasant strategy.


Assuntos
Asteraceae/crescimento & desenvolvimento , Biodegradação Ambiental , Corantes/metabolismo , Desenvolvimento Vegetal/efeitos dos fármacos , Solo/química , Têxteis/análise , Águas Residuárias/química , Asteraceae/efeitos dos fármacos , Asteraceae/fisiologia , Corantes/farmacologia , Jardins , Metais Pesados/análise
18.
Middle East Afr J Ophthalmol ; 25(2): 108-110, 2018 Apr-Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30122857

RESUMO

Over recent years, hand-held optical coherence tomography (HH-OCT) has become critical for retinoblastoma diagnosis and management. We report precise HH-OCT findings in a case of sub-millimeter retinoblastoma treated with foveal-sparing indocyanine green-enhanced transpupillary thermotherapy (ICG-TTT). A 2-month-old Caucasian female with bilateral Group B retinoblastoma showed two recurrent macular tumors in the right eye, demonstrating 88 µm and 37 µm of growth to 344 µm and 413 µm in thickness, respectively, on HH-OCT. Each was treated with additional intravenous chemotherapy and foveal-sparing ICG-TTT. Tumor regression to 154 µm and 224 µm was documented on HH-OCT and maintained on follow-up. HH-OCT is vital in confirming clinical findings and influencing management decisions in retinoblastoma. In this case, HH-OCT precisely documented submillimeter retinoblastoma recurrence and treatment response.


Assuntos
Hipertermia Induzida/métodos , Verde de Indocianina/farmacologia , Neoplasias da Retina/diagnóstico , Retinoblastoma/diagnóstico , Tomografia de Coerência Óptica/instrumentação , Corantes/farmacologia , Desenho de Equipamento , Feminino , Humanos , Lactente , Pupila , Neoplasias da Retina/terapia , Retinoblastoma/terapia
19.
Chem Biodivers ; 15(10): e1800302, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30074284

RESUMO

A quantitative analysis by confocal fluorescence microscopy of the entry into HEK293 and MCF-7 cells by fluorescein-labeled octaarginine (1) and by three octa-Adp derivatives (2 - 4, octamers of the ß-Asp-Arg-dipeptide, derived from the biopolymer cyanophycin) is described, including the effects of the membrane dye R18 and of DMSO on cell penetration.


Assuntos
Proteínas de Bactérias/farmacocinética , Permeabilidade da Membrana Celular/efeitos dos fármacos , Corantes/farmacologia , Dimetil Sulfóxido/farmacologia , Guanidina/farmacocinética , Oligopeptídeos/farmacocinética , Proteínas de Bactérias/química , Guanidina/química , Células HEK293 , Humanos , Células MCF-7 , Oligopeptídeos/química
20.
Jpn J Ophthalmol ; 62(5): 592-597, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30030722

RESUMO

PURPOSE: A surgical technique is described that facilitates membrane peeling in patients with macular diseases by using small amounts of perfluorocarbon liquid (PFCL) and brilliant blue G (BBG) dye. STUDY DESIGN: Retrospective cohort study. METHODS: After placing about 1.0-1.5 cc PFCL on the macular area, BBG was applied with a 27-gauge blunt needle at the interface of PFCL and retina. The membrane peeling was performed under PFCL. RESULTS: The amount of BBG dye with PFCL-assisted staining technique has the potential to be smaller than in conventional staining method. Since the displacement angle of the membrane during peeling procedures is considerably increased by PFCL, combined with its high specific gravity and interfacial tension, the risk of tearing the membrane during manipulation is reduced to a great extent. The postoperative visual function in patients with PFCL-assisted peeling was comparable to that of patients without PFCL-assisted peeling. CONCLUSION: The PFCL-assisted technique enables sufficient membrane staining with minimal amounts of BBG dye, with tearing of the epiretinal membrane or internal limiting membrane being less likely than with the conventional method.


Assuntos
Membrana Basal/cirurgia , Corantes/farmacologia , Membrana Epirretiniana/cirurgia , Fluorcarbonetos/administração & dosagem , Perfurações Retinianas/cirurgia , Coloração e Rotulagem/métodos , Vitrectomia/métodos , Idoso , Idoso de 80 Anos ou mais , Membrana Basal/diagnóstico por imagem , Membrana Epirretiniana/diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Perfurações Retinianas/diagnóstico , Estudos Retrospectivos , Acuidade Visual
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