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1.
Zhongguo Zhong Yao Za Zhi ; 46(12): 3058-3065, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34467696

RESUMO

In this study, the compound search was completed through SciFinder and CNKI databases, and the drug-like properties were screened in FAFdrugs4 and SEA Search Server databases. In addition, based on the target sets related to acute myocardial ischemia(AMI) searched in disease target databases such as OMIM database, GeneCards database and DrugBank, a network diagram of chemical component-target-pathway-disease was established via Cytoscape to predict the potential active components of Corydalis Herba, a traditional Tibetan herbal medicine which derived from the aerial parts of Corydalis hendersonii and C. mucronifera against AMI. A protein-protein interaction(PPI) network was constructed through the STRING database and the core targets in the network were predicted. And the enrichment analyses of core targets were completed by DAVID database and R software. Furthermore, a molecular docking method was used to verify the binding of the components with core targets using softwares such as Autodock Vina. The present results showed that there were 60 compounds related to AMI in Corydalis Herba, involving 73 potential targets. The GO functional enrichment analysis obtained 282 biological processes(BP), 49 cell components(CC) and 78 molecular functions(MF). KEGG was enriched into 85 pathways, including alcoholism pathway, endocrine resistance pathway, calcium signaling pathway, cAMP signaling pathway, vascular endothelial growth factor signaling pathway and adrenergic signaling transduction pathway of myocardial cells. The results of network topology analysis showed that the key components of anti-AMI of Corydalis Herba might be tetrahydropalmatine, etrahydrocolumbamine, N-trans-feruloyloctopamine, N-cis-p-coumaroyloctopamine, N-trans-p-coumaroylnoradrenline and N-trans-p-coumaroyloctopamine, and their core targets might be CDH23, SCN4 B and NFASC. The results of molecular docking showed that the key components of Corydalis Herba had stable binding activity with the core targets. This study provides reference for further elucidation of the pharmacological effects of Corydalis Herba against AMI, subsequent clinical application, and development.


Assuntos
Corydalis , Medicamentos de Ervas Chinesas , Isquemia Miocárdica , Medicamentos de Ervas Chinesas/farmacologia , Medicina Tradicional Tibetana , Simulação de Acoplamento Molecular , Isquemia Miocárdica/tratamento farmacológico , Fator A de Crescimento do Endotélio Vascular
2.
J Pharm Biomed Anal ; 198: 114014, 2021 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-33765511

RESUMO

Jinlingzi San (JLZS), composed of Fructus Toosendan (FT) and Rhizoma Corydalis (RC), is a classical traditional Chinese medicine prescription for regulating Qi to relieve pain. The present study investigated the pharmacokinetic compatibility of FT and RC in JLZS. A fast, selective and sensitive UPLC-MS/MS method for simultaneous determination of one limonoid (toosendanin), four tertiary alkaloids (corydaline, tetrahydropalmatine, tetrahydrocoptisine, tetrahydroberberine) and two quaternary alkaloids (palmatine, dehydrocorydaline) in rat plasma was established and fully validated. The plasma samples were pretreated by a fast protein precipitation and chromatographed using a 1.7-µm C18 column and 0.1 % formic acid-water and acetonitrile via gradient elution with a run time of 3.7 min. Multiple reaction monitoring mode with positive electrospray ionization was adopted to detect the analytes and internal standard (diphenhydramine). The lower limits of quantification were 0.08-3.09 ng/mL using only 50 µL of plasma sample. Using the proposed method, the pharmacokinetic differences of seven bioactive components in rats after administration of JLZS and the single herb (FT or RC) were investigated. The results showed that the elimination of toosendanin and alkaloids decreased significantly in the JLZS group (p < 0.05) compared with the single herb group, and the exposure of the alkaloids increased in some degree. The study demonstrated the synergistic effect of combining FT with RC on the pharmacokinetics of seven bioactive components and provided new information for a better understanding of the compatibility mechanism of JLZS.


Assuntos
Corydalis , Medicamentos de Ervas Chinesas , Animais , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem
3.
Zhongguo Zhong Yao Za Zhi ; 46(5): 1117-1119, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33787104

RESUMO

Based on the investigation of wild medicinal plant resources in Dexing city, Jiangxi province, and the collected plant specimens, which were identified by taxonomy, two new record species of geographical distribution were found, which are Meehania zheminensis A. Takano, Pan Li & G.-H. Xia and Corydalis huangshanensis L.Q.Huang & H.S.Peng. The voucher specimens are kept in Dexing museum of traditional Chinese medicine. In this paper, the new distribution species were reported, which provides valuable information for further enriching and supplementing the species diversity of medicinal plant resources in Jiangxi province.


Assuntos
Corydalis , Lamiaceae , Plantas Medicinais , China , Humanos , Medicina Tradicional Chinesa , Museus
5.
Biosci Biotechnol Biochem ; 85(1): 61-68, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33577669

RESUMO

Corydalis ambigua (Japanese name, Ezoengosaku) flowers bloom with blue to purplish petals in early spring in Hokkaido prefecture. In this study, a mechanism for blue petal coloration by ferric ions and keampferol glycoside was elucidated. Blue petals and cell sap exhibited similar visible (Vis) spectra, with λmax at approximately 600 nm and circular dichroism (CD) with positive exciton-type Cotton effects in the Vis region. Analysis of the organic components of the petals confirmed cyanidin 3-O-sambubioside and kaempferol 3-O-sambubioside as the major flavonoids. Mg, Al, and Fe were detected in petals using atomic emission spectroscopy. Color, Vis absorption, and CD consistent with those of blue petals were reproduced by mixing cyanidin 3-O-sambubioside, kaempferol 3-O-sambubioside, and Fe3+ in a buffered aqueous solution at pH 6.5. Both Fe3+ and flavonol were essential for blue coloration.


Assuntos
Corydalis/metabolismo , Compostos Férricos/metabolismo , Flores/metabolismo , Glicosídeos/química , Quempferóis/química , Quempferóis/metabolismo , Pigmentação
6.
Phytochemistry ; 183: 112644, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33429352

RESUMO

The genus Corydalis is a botanical source of various pharmaceutically active components. Its member species have been widely used in traditional medicine systems in Southeast Asia, especially in China for thousands of years. They have been administered to treat the common cold, hypertension, hepatitis, hemorrhage, edema, gastritis, cardiovascular and cerebrovascular diseases, and neurological disorders. Analgesia is the most important effect of Corydalis products, which are relatively non-addictive and associated with low tolerance compared with other analgesics. Certain Corydalis species are rich in alkaloids, which have strong biological activity, and also contain coumarins, flavonoids, steroids, organic acids and other chemical components. These constituents have pharmacological efficacy against diseases of the nervous, cardiovascular and digestive systems. Numerous investigations have been performed on these plants and their components. Here, we systemically summarized the chemical constituents of important medicinal member species of Corydalis that have been reported since 1962. A total 381 alkaloids were enumerated, including 117 quaternary isoquinoline type, 60 Benzophenanthridine type, 37 aporphine type, 10 protopine type, 59 phthalide isoquinoline type, 52 simple isoquinoline-type, 25 lignin amides and 21 other alkaloids. Thus, we have provided a basis for further explorations into the pharmacologically active constituents of Corydalissp.(Papaveraceae) to develop medicines that exert strong effects, are relatively non-addictive, and result in few side effects.


Assuntos
Alcaloides , Corydalis , Plantas Medicinais , Alcaloides/farmacologia , China , Medicina Tradicional
7.
Fitoterapia ; 147: 104776, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33166598

RESUMO

Three pairs of enantiomers mucroniferals A-C (1-3), with a novel skeleton of 1,4-epoxynaphthalene-2,3-dicarboxylic acid first reported from nature source, were isolated from Corydalis mucronifera. Their structures were elucidated based on extensive spectroscopic data analysis of MS, 1D and 2D NMR, and their absolute configurations were confirmed by single-crystal X-ray diffraction analysis and comparison of the experimental and calculated ECD data. Mucroniferals A-C showed broad-spectrum inhibitory activities on seedling growth of all plants tested (Lepidium apetalum, Raphanus sativus, Lactuca sativa, and Arabidopsis thaliana) with a dose-dependent relationship. Additionally, mucroniferals A and B exhibited significant inhibitory effects on germination of most seeds at concentration of 80 µg/mL, and the inhibition was reversible.


Assuntos
Corydalis/química , Germinação/efeitos dos fármacos , Reguladores de Crescimento de Plantas/farmacologia , Plântula/efeitos dos fármacos , Arabidopsis/efeitos dos fármacos , Lepidium/efeitos dos fármacos , Alface/efeitos dos fármacos , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Reguladores de Crescimento de Plantas/isolamento & purificação , Raphanus/efeitos dos fármacos , Tibet
8.
Bioorg Chem ; 104: 104251, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32920361

RESUMO

Two novel Diels-Alder [4 + 2] cycloadducts of quaternary protoberberine alkaloids and fumaric acid monoanion, corydecumbenines A and B (1 and 2), and six known isoquinoline analogues (3-8) were isolated from the rhizomes of Corydalis decumbens. The planar structures of 1 and 2 were elucidated by extensive spectroscopic analysis including UV, IR, HRESIMS, 1D and 2D NMR. Chiral chromatography of 1 and 2 afforded two pairs of enantiomers (+)-corydecumbenine A (1a), (-)-corydecumbenine A (1b), (+)-corydecumbenine B (2a), and (-)-corydecumbenine B (2b), respectively, and their absolute configurations were determined by single-crystal X-ray crystallography and comparison of experimental and calculated electronic circular dichroism (ECD) spectra. Compounds 1b and 2b exhibited significant nitric oxide (NO) inhibitory activities in lipopolysaccharide (LPS)-stimulated BV-2 cells with IC50 values of 11.6 and 16.2 µM, respectively, comparable to the positive control indomethacin (IC50 = 10.3 µM), and they could also decrease the level of interleukin (IL)-1ß in BV-2 cells in a dose-dependent manner. Most of the isolates showed neuroprotective effects against the injury of OGD/R-induced PC12 cells at 20 µM.


Assuntos
Anti-Inflamatórios/farmacologia , Corydalis/química , Fármacos Neuroprotetores/farmacologia , Rizoma/química , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Glucose/metabolismo , Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Camundongos , Estrutura Molecular , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/isolamento & purificação , Óxido Nítrico/antagonistas & inibidores , Óxido Nítrico/biossíntese , Oxigênio/metabolismo , Células PC12 , Ratos , Estereoisomerismo , Relação Estrutura-Atividade
9.
Molecules ; 25(18)2020 09 07.
Artigo em Inglês | MEDLINE | ID: mdl-32906783

RESUMO

With the increase in demand, artificially planting Chinese medicinal materials (CHMs) has also increased, and the ensuing pesticide residue problems have attracted more and more attention. An optimized quick, easy, cheap, effective, rugged and safe (QuEChERS) method with multi-walled carbon nanotubes as dispersive solid-phase extraction sorbents coupled with surface-enhanced Raman spectroscopy (SERS) was first proposed for the detection of deltamethrin in complex matrix Corydalis yanhusuo. Our results demonstrate that using the optimized QuEChERS method could effectively extract the analyte and reduce background interference from Corydalis. Facile synthesized gold nanoparticles with a large diameter of 75 nm had a strong SERS enhancement for deltamethrin determination. The best prediction model was established with partial least squares regression of the SERS spectra ranges of 545~573 cm-1 and 987~1011 cm-1 with a coefficient of determination (R2) of 0.9306, a detection limit of 0.484 mg/L and a residual predictive deviation of 3.046. In summary, this article provides a new rapid and effective method for the detection of pesticide residues in CHMs.


Assuntos
Corydalis/química , Nanotubos de Carbono/química , Nitrilas/análise , Resíduos de Praguicidas/análise , Piretrinas/análise , Análise Espectral Raman , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/química , Modelos Moleculares , Estrutura Molecular , Nanotubos de Carbono/ultraestrutura , Nitrilas/química , Nitrilas/isolamento & purificação , Resíduos de Praguicidas/química , Resíduos de Praguicidas/isolamento & purificação , Piretrinas/química , Piretrinas/isolamento & purificação , Reprodutibilidade dos Testes
10.
Molecules ; 25(16)2020 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-32784618

RESUMO

Corydalis and Pseudofumaria are two closely related genera from the Papaveraceae subfamily Fumarioideae with Corydalis solida (C. solida) and Pseudofumaria lutea (P. lutea) as two representative species. Phytochemical analysis revealed significant differences in the quality and quantity of isoquinoline alkaloids, phenolic compounds and non-phenolic carboxylic acids between aerial and underground parts of both species. Using the Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) technique, 21 compounds were identified: five protoberberine derivatives, three protopine derivatives, four phenanthridine derivatives, as well as three carboxylic acids, two hydroxycinnamic acids, one chlorogenic acid, one phenolic aldehyde, and two flavonoids. Moroever, significant differences in the content of individual compounds were observed between the two studied species. The phytochemical profile of C. solida showed a higher variety of compounds that were present in lower amounts, whereas P. lutea extracts contained fewer compounds but in larger quantities. Protopine was one of the most abundant constituents in C. solida (440-1125 µg/g d.w.) and in P. lutea (1036-1934 µg/g d.w.). Moreover, considerable amounts of coptisine (1526 µg/g) and quercetin (3247 µg/g) were detected in the aerial parts of P. lutea. Extracts from aerial and underground parts of both species were also examined for the antimicrobial potential against S. aureus, P. aeruginosa and C. albicans. P. lutea herb extract was the most effective (MIC at 0.39 mg/L) against all three pathogens.


Assuntos
Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Corydalis/química , Compostos Fitoquímicos/análise , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Candida albicans/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos
11.
J Chromatogr A ; 1625: 461294, 2020 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-32709337

RESUMO

It is significant to precisely isolate potential active compounds from medicinal herbs containing multiple compounds. Herein, a new strategy for precise separation of lysine-specific demethylase 1 (LSD1) inhibitors from the rhizome of Corydalis yanhusuo (RCY) using counter-current chromatography (CCC) guided by molecular docking and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis was established. First, representative alkaloids from RCY were docked with LSD1 for screening active skeleton compounds. Simultaneously, the crude extract of RCY was preliminarily separated via pH-zone refining CCC. Subsequently, guided by LC-MS/MS analysis of the fragmentation pathways, three potential active fractions were obtained, followed by further online-storage and recycling CCC separation. Finally, three high-purity target quaternary alkaloids compound 3 (dehydrocorydaline), 7 (coptisine), and 8 (columbamine) were successfully isolated as a new class of potential natural LSD1 inhibitors by only one CCC instrument with multiple modes. Compound 3, with the highest LSD1 inhibition ratio of 2.44 µM, was tested for its ability to inhibit tumor invasion and metastasis in U2OS cells. Therefore, the CCC separation guided by virtual screening is a promising method for the targeted isolation of enzyme inhibitors from medicinal herbs.


Assuntos
Corydalis/química , Distribuição Contracorrente/métodos , Avaliação Pré-Clínica de Medicamentos , Inibidores Enzimáticos/isolamento & purificação , Histona Desmetilases/antagonistas & inibidores , Interface Usuário-Computador , Bioensaio , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Inibidores Enzimáticos/química , Histona Desmetilases/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Simulação de Acoplamento Molecular , Solventes , Espectrometria de Massas em Tandem
12.
Zhongguo Zhong Yao Za Zhi ; 45(11): 2586-2594, 2020 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-32627493

RESUMO

Corydalis Bungeanae Herba is often used to treat a variety of inflammatory diseases in traditional Chinese medicine. In order to determine its chemical material basis, the components of Corydalis Bungeanae Herba were isolated by automated purification system. Flavonoids and alkaloids were prepared, and all such components were identified by mass spectrometry. The effects of the components on the production of inflammatory mediators and pharmacological mechanisms in the lipopolysaccharide(LPS)-induced RAW264.7 cell inflammation model were examined. Mouse macrophages(RAW264.7) were first treated with LPS. The relationship between cell viability and LPS concentration was observed. Then, the effects of flavonoids components and alkaloid components with different administration concentrations on cell viability were detected to determine the maximum administration concentration. Secondly, 2.5, 5, 10 and 20 µg·mL~(-1) flavonoids components and alkaloid components were added respectively to observe the effects and mechanism of different concentrations of flavonoids components and alkaloid components on LPS-induced inflammation of RAW264.7 macrophages. Griess reagent assay was used to detect NO content in cell supernatant. The inflammatory cytokines(TNF-α, IL-1ß and IL-6) in cell supernatant were determined by ELISA method. Western blot method was used to detect the intracellular nuclear factor(NF-κB) IκBα phosphorylation(p-IκBα), p65 phosphorylation(p-p65) and protein expression of TLR4, TLR2. The results showed that the alkaloid components inhibited the production of NO, TNF-α, IL-1ß and IL-6 in a dose-dependent mannerin the concentration range of 2.5-20 µg·mL~(-1). In inflammation upstream pathways, the inhibitory effect of the alkaloid components on the TLR2 expression level was weaker than that of TLR4. In inflammation downstream, alkaloid components significantly inhibited phosphorylation of IκBα and p65 in a dose-dependent manner. These data suggested that the alkaloid components were the material basis components of Corydalis Bungeanae Herba, and its anti-inflammatory mechanism might be related to inhibiting the transmission of inflammatory signals in TLRs/NF-κB signaling pathways dominated by TLR4, interfering with the activation of inflammatory genes and inhibiting their over expression, and down-regulating the secretion level of inflammatory factors.


Assuntos
Corydalis , Animais , Anti-Inflamatórios/uso terapêutico , Inflamação/tratamento farmacológico , Lipopolissacarídeos , Camundongos , NF-kappa B , Células RAW 264.7
13.
Artigo em Inglês | MEDLINE | ID: mdl-32531643

RESUMO

Enantioselective analysis is critically important in the pharmaceutical and agricultural industries. However, most of the methods reported were developed for the analysis of pure racemates acquired from chemical synthesis or purification. Direct analysis of chiral enantiomers in complex matrices has rarely been reported. This work demonstrated capillary electrophoresis-mass spectrometry (CE-MS) for the enantioselective analysis of botanical drugs for the first time, using a widely used botanical drug, Corydalis Rhizoma, as an example. The method was used for the simultaneous enantioselective analysis of dl-tetrahydropalmatine and (RS)-tetrahydroberberine (canadine) in Corydalis Rhizoma extract. Using (2-hydroxypropyl)-ß-cyclodextrin as the chiral selector, a partial filling technique was used to avoid signal suppression and contamination of the MS detector. Post column organic modifier was used to assist with ionization in the flow through microvial CE-MS interface, therefore, organic solvents was not used in the background electrolyte. The completely aqueous background electrolyte contributed to better chiral separations. The CE-MS method established here can directly determine the analytes in their complex matrix without any pre-purification steps, while also offering high sensitivity and low operational costs (including sample, chiral selector and solvent). In the method validation process, good linearity (r > 0.993), sensitivity and accuracy (recoveries within 89.1-110.0%) were demonstrated. The CE-MS technique was shown to be able to provide good selectivity for the simultaneous chiral separation of multiple pairs of enantiomers in complex matrices.


Assuntos
Corydalis/química , Eletroforese Capilar/métodos , Espectrometria de Massas/métodos , Extratos Vegetais/análise , Berberina/análogos & derivados , Berberina/análise , Berberina/química , Alcaloides de Berberina/análise , Alcaloides de Berberina/química , Limite de Detecção , Modelos Lineares , Modelos Químicos , Extratos Vegetais/química , Reprodutibilidade dos Testes , Estereoisomerismo
14.
Zhongguo Zhong Yao Za Zhi ; 45(10): 2411-2416, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32495600

RESUMO

Two new phenylpropanoid amide glycosides and ten analogues were isolated from the CH_2Cl_2 layer of 95% ethanol extract of the whole plants of Corydalis racemosa by using various chromatographic techniques, including silica gel, Sephadex LH-20, ODS column chromatographies, and semi-preparative HPLC. Their structures were identified on the basis of physicochemical properties, MS, NMR, and IR spectroscopic data as N-cis-sinapoyltyramine-4'-O-ß-glucoside(1), N-cis-sinapoyl-3-methoxytyramine-4'-O-ß-glucoside(2), N-cis-sinapoyltyramine(3), N-cis-feruloyltyramine(4), N-trans-cinnamoyltyramine(5), N-trans-feruloylphenethylamine(6), N-trans-p-methoxycinnamoyl-3-hydoxyoctopamine(7), N-cis-feruloyl-3-methoxytyramine(8), N-trans-feruloyltyramine(9), N-trans-feruloyl-3-methoxytyramine(10), N-trans-sinapoyltyramine(11), and N-trans-p-coumaroyltyramine(12). Compounds 1 and 2 are new compounds. Compounds 3-7 are obtained from the plants of Papaveraceae for the first time, and compounds 8-12 are firstly isolated from C. racemosa.


Assuntos
Corydalis , Amidas , Cromatografia Líquida de Alta Pressão , Glucosídeos , Glicosídeos
15.
Ying Yong Sheng Tai Xue Bao ; 31(3): 769-777, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-32537971

RESUMO

We examined the effects of five shading treatments (0, 20%, 40%, 60% and 80% shading) on chlorophyll content, photosynthetic characteristics, and chlorophyll fluorescence chara-cteristics of four Corydalis species (C. incisa, C. decumbens, C. edulis and C. pallida) in a pot experiment. The results showed that the contents of chlorophyll a, chlorophyll b and chlorophyll (a+b) increased with the increment of shading, with that of C. incisa reaching the maximum under 80% shading treatment and that of C. decumbens, C. edulis and C. pallida reaching a maximum under 60% shading treatment. In contrast, chlorophyll a/b, light saturation point, light compensation point and dark respiration rate decreased with increasing shading. Among the four Corydalis species, C. incisa reached up to the maximum chlorophyll fluorescence parameters under 80% shading treatment, and C. decumbens, C. edulis and C. pallida reached the maximum at 60% shading treatment. The shade tolerance of four species was as follows: C. incisa > C. decumbens > C. edulis > C. pallida. C. incisa under 80% shading treatment and C. decumbens, C. edulis, C. pallida at 60% shading treatment had the highest light energy utilization and photosynthetic capacity, which would facilitate their growth.


Assuntos
Corydalis , Clorofila , Clorofila A , Fluorescência , Luz , Fotossíntese , Folhas de Planta
16.
Microb Pathog ; 147: 104270, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32446872

RESUMO

BACKGROUND: The Mycoplasma pneumoniae (M.pneumoniae) was accounted to 3-10% of total pneumonia incidences. In recent decades, metallic nanoparticles were extensively examined as nano-antibiotics. OBJECTIVE: In this investigation, we intended to inspect the therapeutic potential of Zinc oxide nanoparticles (ZnONPs) from (Corydalis yanhusuo) C. yanhusuo against the mycoplasma infected pneumonia in mice. METHODOLOGY: The ZnONPs were formulated via green route technique and characterized by UV-vis spectroscopy, transmission electron microscopy, Fourier transform infrared technique, and atomic force microscopy. The antimicrobial activity of formulated ZnONPs was tested by well diffusion method. The total protein, interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor alpha (TNF-α) and transforming growth factor (TGF) status in the BALF of M. pneumonia infected animals were investigated via kit method. The expressions of ERK1/2, JNK1/2, and NF-κB were examined through the Western blotting. The Histopathological analysis of lung tissues of experimental animals was done. RESULTS: The UV-vis spectroscopy and TEM examinations were proved the existence of CY-ZnONPs. The formulated CY-ZnONPs were displayed the potential antimicrobial activity. The supplementation of CY-ZnONPs were noticeably diminished the total protein and IL-6, IL-8, and TNF-α levels in the BALF of pneumonia mice. The ERK1/2, JNK1/2, and NF-κB expressions were appreciably diminished in the CY-ZnONPs supplemented mice. It also reduced the inflammatory cells penetration, and exhibited normal tissue arrangements in the lung tissues of pneumonia mice. CONCLUSION: The findings of this investigation were proved that the synthesized CY-ZnONPs has the potential to ameliorate the M. pneumoniae infected pneumonia in investigational mice.


Assuntos
Corydalis , Nanopartículas Metálicas , Nanopartículas , Pneumonia por Mycoplasma , Óxido de Zinco , Animais , Sistema de Sinalização das MAP Quinases , Camundongos , Pneumonia por Mycoplasma/tratamento farmacológico , Transdução de Sinais
17.
J Sep Sci ; 43(13): 2521-2528, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32240569

RESUMO

Positively charged reversed-phase liquid chromatography was employed for the efficient preparative separation of isoquinoline alkaloids from Corydalis impatiens. Ten commercially available columns were compared for isoquinoline alkaloids analysis. While tailing, overloading, lower resolution, and buffer salts limited the application in purification of isoquinoline compounds of many of these columns, one positively charged reversed-phase C18 column (XCharge C18) overcame these drawbacks, allowing for favorable separation resolution, even when loading isoquinoline compounds on a larger, preparative scale. The general separation process is as follows. First, isoquinoline alkaloids are enriched with Corydalis impatiens extract via a middle chromatogram isolated gel column. After column selection, separation is performed on an XCharge C18 analytical column, from which two evident chromatographic peaks are readily obtained. Finally, two isoquinoline alkaloids (protopine and corydamine) are selectively purified on the XCharge C18 preparative column. These results demonstrate that a middle chromatogram isolated gel column coupled with positively charged reversed-phase liquid chromatography is effective for the preparative separation of isoquinoline alkaloids from Corydalis impatiens.


Assuntos
Alcaloides/isolamento & purificação , Corydalis/química , Isoquinolinas/isolamento & purificação , Alcaloides/química , Cromatografia de Fase Reversa , Isoquinolinas/química
18.
Pharm Biol ; 58(1): 265-275, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32223481

RESUMO

ABSRACTContext: Rhizoma Corydalis (RC) is the dried tubers of Corydalis yanhusuo (Y. H. Chou and Chun C. Hsu) W. T. Wang ex Z. Y. Su and C. Y. Wu (Papaveraceae). Traditionally, RC is used to alleviate pain such as headache, abdominal pain, and epigastric pain. Modern medicine shows that it has analgesic, anti-arrhythmia, and other effects.Objective: We provided an overview of the phytochemical and pharmacological properties of RC as a foundation for its clinical application and further research and development of new drugs.Methods: We collected data of various phytochemical and pharmacological effects of RC from 1982 to 2019. To correlate with existing scientific evidence, we used Google Scholar and the journal databases Scopus, PubMed, and CNKI. 'Rhizoma Corydalis', 'phytochemistry', and 'pharmacological effects' were used as key words.Results: Currently, more than 100 chemical components have been isolated and identified from RC, among which alkaloid is the pimary active component of RC. Based on prior research, RC has antinociceptive, sedative, anti-epileptic, antidepressive and anti-anxiety, acetylcholinesterase inhibitory effect, drug abstinence, anti-arrhythmic, antimyocardial infarction, dilated coronary artery, cerebral ischaemia reperfusion (I/R) injury protection, antihypertensive, antithrombotic, antigastrointestinal ulcer, liver protection, antimicrobial, anti-inflammation, antiviral, and anticancer effects.Conclusions: RC is reported to be effective in treating a variety of diseases. Current pharmacological studies on RC mainly focus on the nervous, circulatory, digestive, and endocrine systems, as well as drug withdrawal. Although experimental data support the beneficial effects of this drug, its physiological activity remains a concern. Nonetheless, this review provides a foundation for future research.


Assuntos
Corydalis/química , Compostos Fitoquímicos/farmacologia , Preparações de Plantas/farmacologia , Rizoma/química , Animais , Humanos , Medicina Tradicional Chinesa , Compostos Fitoquímicos/isolamento & purificação , Preparações de Plantas/isolamento & purificação
19.
Bioorg Chem ; 99: 103795, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32240871

RESUMO

Eight new alkaloids, including five isoquinoline alkaloids, a benzoazepine alkaloid, two isoindole alkaloids, and three synthetic alkaloids firstly obtained from the natural sources, together with three known ones were isolated from the bulbs of Corydalis decumbens. The structures were determined by analysis of their spectroscopic data and single-crystal X-ray diffraction. This is the first report of isoindole alkaloid and benzoazepine alkaloid from the genus Corydalis. Full NMR data for 9-11 are reported here for the first time. Moreover, the ability to modulate neuronal Ca2+ mobilization of the isolated alkaloids was tested in primary cultured neocortical neurons. Compound 7 inhibited spontaneous Ca2+ oscillations in primary neocortical neuron cultures at low micromolar concentrations.


Assuntos
Alcaloides/farmacologia , Produtos Biológicos/farmacologia , Corydalis/química , Neurônios/efeitos dos fármacos , Alcaloides/química , Alcaloides/isolamento & purificação , Animais , Produtos Biológicos/química , Produtos Biológicos/isolamento & purificação , Cálcio/metabolismo , Cristalografia por Raios X , Relação Dose-Resposta a Droga , Camundongos , Camundongos Endogâmicos C57BL , Modelos Moleculares , Estrutura Molecular , Neurônios/metabolismo , Relação Estrutura-Atividade
20.
Zhongguo Zhong Yao Za Zhi ; 45(3): 579-583, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32237516

RESUMO

This study is to investigate the chemical constituents from the whole plant Corydalis edulis. The chemical constituents were separated and purified by macroporous resin D101, silica gel, Sephadex LH-20, ODS, and semi-preparative HPLC. Their structures were determined by physicochemical properties and spectroscopic data. Four compounds were isolated from the dichloromethane and water extracts of the whole plant C. edulis, and identified as 6'-ß-D-xylosylicariside B2(1),(3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one(2), loliolide(3), and 5,5'-dimethoxybiphenyl-2,2'-diol(4), respectively. Compound 1 is a new compound, of which the absolute configuration was established by electronic circular dichroism(ECD) calculations. Compound 4 is obtained from the plants of Papaveraceae family for the first time. Compounds 2 and 3 are firstly isolated from the Corydalis genus.


Assuntos
Corydalis/química , Glicosídeos/isolamento & purificação , Sesquiterpenos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação
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