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1.
Molecules ; 26(5)2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33652694

RESUMO

Colorectal cancer (CRC) is the third most common type of cancer worldwide and a leading cause of cancer death. According to the Malaysian National Cancer Registry Report 2012-2016, colorectal cancer was the second most common cancer in Malaysia after breast cancer. Recent treatments for colon cancer cases have caused side effects and recurrence in patients. One of the alternative ways to fight cancer is by using natural products. Curcumin is a compound of the rhizomes of Curcuma longa that possesses a broad range of pharmacological activities. Curcumin has been studied for decades but due to its low bioavailability, its usage as a therapeutic agent has been compromised. This has led to the development of a chemically synthesized curcuminoid analogue, (2E,6E)-2,6-bis(2,3-dimethoxybenzylidine) cyclohexanone (DMCH), to overcome the drawbacks. This study aims to examine the potential of DMCH for cytotoxicity, apoptosis induction, and activation of apoptosis-related proteins on the colon cancer cell lines HT29 and SW620. The cytotoxic activity of DMCH was evaluated using the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) cell viability assay on both of the cell lines, HT29 and SW620. To determine the mode of cell death, an acridine orange/propidium iodide (AO/PI) assay was conducted, followed by Annexin V/FITC, cell cycle analysis, and JC-1 assay using a flow cytometer. A proteome profiler angiogenesis assay was conducted to determine the protein expression. The inhibitory concentration (IC50) of DMCH in SW620 and HT29 was 7.50 ± 1.19 and 9.80 ± 0.55 µg/mL, respectively. The treated cells displayed morphological features characteristic of apoptosis. The flow cytometry analysis confirmed that DMCH induced apoptosis as shown by an increase in the sub-G0/G1 population and an increase in the early apoptosis and late apoptosis populations compared with untreated cells. A higher number of apoptotic cells were observed on treated SW620 cells as compared to HT29 cells. Human apoptosis proteome profiler analysis revealed upregulation of Bax and Bad proteins and downregulation of Livin proteins in both the HT29 and SW620 cell lines. Collectively, DMCH induced cell death via apoptosis, and the effect was more pronounced on SW620 metastatic colon cancer cells, suggesting its potential effects as an antimetastatic agent targeting colon cancer cells.


Assuntos
Antineoplásicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Neoplasias do Colo/tratamento farmacológico , Curcumina/farmacologia , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Proteínas Reguladoras de Apoptose/genética , Sobrevivência Celular/efeitos dos fármacos , Neoplasias do Colo/patologia , Curcuma/química , Curcumina/análogos & derivados , Curcumina/química , Diarileptanoides/química , Diarileptanoides/farmacologia , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Células HT29 , Humanos , Extratos Vegetais/química , Extratos Vegetais/farmacologia
2.
Zhongguo Zhong Yao Za Zhi ; 46(6): 1393-1400, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33787137

RESUMO

L~*, a~* and b~* values of prepared slices of Curcumae Rhizoma were measured by spectrophotometer. SPSS 21.0 was used for discriminant analysis to establish the color range and mathematical prediction model of prepared slices of Curcumae Rhizoma. The values of L~*, a~* and b~* of kwangsiensis ranged from 58.09-62.40, 4.53-5.66 and 23.61-24.29, while the values of L~*, a~* and b~* of phaeocaulis were between 64.02-70.71,-0.89-4.13 and 44.59-54.52, respectively. The values of L~*, a~* and b~* of wenyujin were 68.55-70.99,-0.11-1.47 and 28.26-32.19, respectively. The mathematical prediction model was proved to be able to realize 100% identification of Curcumae Rhizome of different origins through original and cross validation and external samples validation. A dual wavelength HPLC was established; the contents of 9 sesquiterpenoids and 3 Curcumae Rhizomes were determined simultaneously; and the contents of Curcumae Rhizome of different origins were determined. The results showed that kwangsiensis had higher contents of neocurdione, ß-elemene and isocurcumaenol, phaeocaulis curcumin, furadienone, demethoxycurcumin and curcumin; and wenyujin mainly contained curdione, furadienes and guimarone. Pearson correlation analysis on L~*, a~*, b~* value and content of 12 components showed that curcumin, furadienone, demethoxycurcumin and curcumin had a significant positive correlation with b~* value(P<0.01). There was a significant negative correlation between neocurdione, ß-elemene and isocurcumaenol and L~* value(P<0.01). Curdione, furadienes and guimarone were significantly correlated with L~* value(P<0.01),indicating that the appearance co-lor of Curcumae Rhizoma could reflect the change of the content of the internal components. This study provided reference for the rapid recognition of Curcumae Rhizoma and the establishment of quality evaluation system.


Assuntos
Curcumina , Rizoma , Cromatografia Líquida de Alta Pressão , Cor , Curcuma
3.
Trials ; 22(1): 105, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-33516238

RESUMO

BACKGROUND: To compare the efficacy and safety of bioavailable turmeric extract versus paracetamol in patients with knee osteoarthritis (OA). METHODS: In this randomized, non-inferiority, controlled clinical study, patients of knee OA were randomized to receive bioavailable turmeric extract (BCM-95®) 500 mg capsule two times daily or paracetamol 650 mg tablet three times daily for 6 weeks. The primary outcome measure was Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain subscale. The secondary outcome measures were WOMAC total, WOMAC stiffness, and WOMAC physical function scores. Responder analysis of individual patients at different levels (≥ 20%, ≥ 50%, and ≥ 70%) for WOMAC score was calculated. TNF alpha and CRP levels were evaluated and adverse events (AE) were also recorded. RESULTS: Seventy-one and seventy-three knee OA patients, respectively in bioavailable turmeric extract and paracetamol groups, completed the study. Non-inferiority (equivalence) test showed that WOMAC scores were equivalent in both the groups (p value < 0.05) in all the domains within the equivalence limit defined by effect size (Cohen's d) of 0.5 whereas CRP and TNF-α were better reduced with turmeric extract than paracetamol. After 6 weeks of treatment, WOMAC total score, pain, stiffness, and function scores got a significant improvement of 23.59, 32.09, 28.5, and 20.25% respectively with turmeric extract. In the turmeric extract group, 18% of patients got more than 50% improvement and 3% of patients got more than 70% improvement in WOMAC pain and function/stiffness score and none of the patients in the paracetamol group met the criteria. CRP and TNF-α got significantly reduced (37.21 and 74.81% respectively) in the turmeric extract group. Adverse events reported were mild and comparatively less in the turmeric extract group (5.48%) than in the paracetamol group (12.68%). CONCLUSION: The results of the study suggest that bioavailable turmeric extract is as effective as paracetamol in reducing pain and other symptoms of knee osteoarthritis and found to be safe and more effective in reducing CRP and TNF-α. TRIAL REGISTRATION: Clinical Trials Registry - India CTRI/2017/02/007962 . Registered on 27 February 2017.


Assuntos
Acetaminofen/uso terapêutico , Osteoartrite do Joelho/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Acetaminofen/efeitos adversos , Adulto , Curcuma/efeitos adversos , Método Duplo-Cego , Feminino , Humanos , Índia , Masculino , Pessoa de Meia-Idade , Medição da Dor , Extratos Vegetais/efeitos adversos , Estudos Prospectivos , Resultado do Tratamento
4.
Ecotoxicol Environ Saf ; 208: 111739, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396067

RESUMO

Ultraviolet-B is an important fraction of sunlight which influences the plant performance either positively or adversely in terms of growth, physiology, biochemistry, and major active compounds. The static nature of plants constrains them to be subjected to various adverse environmental conditions. Several studies performed with plants and UV-B with fewer reports are available on medicinal plants having rhizome. The present study focuses on transformation induced in two Curcuma spp. (C. caesia and C. longa) under the influence of elevated UV-B (eUV-B) (ambient ±9.6 kJ m-2 d-1) under natural field conditions to analyse the changes in physiological, biochemical and essential oil of the test plants. eUV-B significantly reduced the photosynthetic activities such as photosynthetic rate (Ps), stomatal conductance (gs), transpiration (Tr), internal CO2 (Ci), and photochemical efficiency (Fv/Fm) with higher reductions in C. longa as compared to C. caesia. The enzymatic activities of PAL, CHI, and CAD showed higher stimulation in C. caesia whereas C. longa showed increment only in CAD. The essential oil content was increased by 16% and 9% in C. caesia and C. longa, respectively. C. caesia showed increased monoterpenes than sesquiterpenes, whereas almost equal increase of both the terpenoid found in C. longa. C. caesia showed induction of aromatic compounds (epiglobulol, germacrene, 4-terpineol), whereas anticancerous compounds; aphla-terpinolene (61%), beta-caryophyllene (60%), and beta-sesquiphellandrene (32%) were increased in C. longa. C. caesia acted well in terms of both physiology and major active compound (1, 8-cineole), but overall most of the compounds increased in C. longa under eUV-B.


Assuntos
Curcuma/efeitos da radiação , Raios Ultravioleta , Curcuma/química , Curcuma/fisiologia , Óleos Voláteis/farmacologia , Fotossíntese/efeitos dos fármacos , Extratos Vegetais , Plantas/efeitos dos fármacos , Rizoma/química , Terpenos
5.
Ecotoxicol Environ Saf ; 209: 111785, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33348254

RESUMO

Heavy metals are considered contaminants that hazardously influence the healthy life of humans and animals as they are widely used in industry. Contact of youngsters and women at ages of parturition with lead (Pb+2) is a main related concern, which passes through the placental barricade and its better absorption in the intestine leads to flaws in the fetal developfment. However, the metals threaten animal and human life, in particular throughout developmental stages. Products existing in the nature have a major contribution to innovating chemo-preventives. As a naturally available polyphenol and necessary curcuminoid, curcumin (Cur) is a derivative of the herb Curcuma longa (L.) rhizome, which globally recognized as "wonder drug of life"; however, Cur has a limited clinical use as it is poorly dissolved in water. Therefore, to enhance its clinically relevant parameters, curcumin-loaded calcium carbonate (CaCO3@Cur) was synthesized by one step coprecipitation method as a newly introduced in this research. Initially, its structure was physio chemically characterized using FT-IR, FESEM and DLS equipment and then the cytotoxicity of lead when it was pretreated with Cur/CaCO3@Cur were assessed by MTT assay. Both Cur and CaCO3@Cur diminished the toxic effects of Pb+2 while the most protective effect on the Pb+2 cytotoxicity was achieved by pre-incubation of cells with CaCO3@Cur. Besides, the morphological changes of Pb+2-treated cells that were pre-incubated with or without Cur/CaCO3@Cur were observed by normal and florescent microscopes. A non-pharmacologic method that lowers the hazard of brain damage is exercise training that is capable of both improving and alleviating memory. In the current study, the role of regular aerobic training and CaCO3@Cur was assessed in reducing the risk of brain damage induced by lead nitrate contact. To achieve the mentioned goal, pregnant Balb/C mice were assigned to five groups (six mice/group) at random: negative and positive controls, aerobic training group and Cur and CaCO3@Cur treated (50 mg/kg/b.wt) trained groups that exposed to Pb+2 (2 mg/kg) by drinking water during breeding and pregnancy. With the completion of study, offspring were subjected to the behavioral tasks that was tested by step-through ORT, DLB, MWM and YM tests. As a result, having regular aerobic training and CaCO3@Cur co-administration with lead nitrate could reverse the most defected behavioral indicators; yet, this was not visible for both sexes and it seems that gender can also be a source of different effects in the animal's body. In fact, having regular aerobic training along with CaCO3@Cur supplementation during pregnancy may be encouraging protecting potential agents towards the toxicity of Pb+2 that could be recommended in the areas with high pollution of heavy metals.


Assuntos
Carbonato de Cálcio , Disfunção Cognitiva/prevenção & controle , Curcumina , Suplementos Nutricionais , Substâncias Perigosas/toxicidade , Chumbo/toxicidade , Animais , Curcuma/efeitos dos fármacos , Feminino , Humanos , Masculino , Camundongos , Nitratos , Extratos Vegetais , Gravidez , Espectroscopia de Infravermelho com Transformada de Fourier
6.
J Ethnopharmacol ; 269: 113689, 2021 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33309584

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Curcuma wenyujin is a multifunctional medicinal plant belonging to the ginger family (Zingiberaceae). It has been used to treat blood stasis, promote the flow of qi, dredge the meridians, and relieve pain for more than 1500 years. Its raw rhizomes, steamed rhizomes, and steamed roots constitute three herbal medicines currently listed in the Chinese Pharmacopoeia: pian-jiang-huang (), wen-e-zhu () and wen-yu-jin (), respectively. AIM OF THE REVIEW: The aim of this review was to comprehensively summarize the traditional use, phytochemistry, and pharmacology of C. wenyujin in order to provide theoretical support for its further investigation and utilization. MATERIALS AND METHODS: Multiple databases (Scifinder, CNKI, Web of Science, PubMed, Google Scholar, and Baidu Scholar) were searched. Some information was also obtained from the literatures on traditional Chinese medicine. RESULTS: A total of 169 compounds have been isolated from C. wenyujin so far. Sesquiterpenoids are the major constituents and are crucial chemotaxonomic markers. Modern pharmacological studies and clinical trials have demonstrated that the extracts or active compounds from C. wenyujin have anti-inflammatory, antitumor, antioxidant, antibacterial, antiviral, and hepatoprotective properties. CONCLUSIONS: Until now, significant progress has been witnessed in phytochemistry and pharmacology of C. wenyujin. Some traditional uses of C. wenyujin have been supported by modern pharmacological studies. However, the establishment of quality control standards and additional clinical studies are warranted.


Assuntos
Curcuma , Medicamentos de Ervas Chinesas/uso terapêutico , Etnofarmacologia/métodos , Medicina Tradicional Chinesa/métodos , Compostos Fitoquímicos/uso terapêutico , Animais , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Antineoplásicos Fitogênicos/uso terapêutico , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico
7.
Food Chem ; 345: 128772, 2021 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-33310255

RESUMO

We developed a whey protein admixture of turmeric extract by spray drying (TWPC-SD) and by foam mat drying (TWPC-FMD) and compared its bioactive compounds and nutrients contents. TWPC samples were evaluated for preference and acceptability. Vitamins and carotenoids were determined by high-performance liquid chromatography. Total phenolics, curcumin and antioxidant capacity were determined by spectrophotometry. Centesimal composition was performed according to the Association of Official Analytical Chemists. Chemical elements were determined by inductively coupled plasma optical emission spectrometry. TWPC containing 3.6 mg of curcumin showed good acceptability. Carotenoids and riboflavin were not detected in either TWPC. Vitamin C content was maintained, and antioxidant capacity was increased in both products (p < 0.05). TWPC-SD showed higher total phenolic and curcumin contents compared to TWPC-FMD (p < 0.05). Thus, the TWPC-SD is a good alternative for human consumption since it showed good sensory acceptability and its nutrients and bioactive compounds can contribute to human health.


Assuntos
Misturas Complexas/química , Curcuma/química , Nutrientes/análise , Extratos Vegetais/química , Proteínas do Soro do Leite/química , Antioxidantes/análise , Ácido Ascórbico/análise , Carotenoides/análise , Cromatografia Líquida de Alta Pressão , Dessecação , Humanos , Fenóis/análise , Vitaminas/análise
8.
Food Chem ; 339: 128121, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33152891

RESUMO

We investigated how various drying methods affect the chemical components and microstructure of turmeric. We used freeze drying (FD), hot-air drying (HD) at 50 °C and sun drying (SD). Compared to all dried samples, fresh turmeric had greater amounts of chemical components (curcuminoids, phenolic acids, flavonoids and amino acids) andgreater antioxidantactivity (DPPH and FRAP) except for ABTS.+ values. SD caused a severe decline of three major curcuminoids, resulting from UV and light degradation. Curcumin was decreased the most by SD (72%), followed by HD (61%) and FD (55%), respectively. Curcumin degradation to ferulic acid and vanillin was explored by FTIR analysis. The amino acids were decreased by all drying methods. FD was considered to be most suitable for drying turmeric. Microstructure changes were least after freeze drying, but were still evident compared with fresh turmeric.


Assuntos
Curcuma/química , Diarileptanoides/química , Liofilização/métodos , Aminoácidos/análise , Antioxidantes/análise , Antioxidantes/química , Temperatura Baixa , Curcumina/química , Flavonoides/análise , Fenóis/análise , Luz Solar , Temperatura
9.
Food Chem ; 339: 128140, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33152894

RESUMO

Curcumin was extracted from Curcuma Longa employing a green, bio-based, and food-agreed surfactant-free microemulsion (SFME) consisting of water, ethanol, and triacetin. Concerning the high solubility of curcumin in the examined ternary mixtures, it was attempted to produce highly concentrated tinctures of up to a total of ~130 mg/mL curcuminoids in the solvent by repeatedly extracting fresh rhizomes in the same extraction mixture. The amount of water had a significant influence on the number of cycles that could be performed as well as on the extraction of the different curcuminoids. In addition, the purity of single extracts was enhanced to 94% by investigating several purification steps, e.g. vacuum distillation and lyophilization. Through purification before extraction, the water insoluble curcumin extract could be solubilized indefinitely in an aqueous environment. Additional stability tests showed that solutions of curcumin can be stable up to five months when concealed from natural light.


Assuntos
Curcuma/química , Curcumina/química , Emulsões/química , Extratos Vegetais/química , Curcumina/isolamento & purificação , Diarileptanoides/química , Etanol/química , Liofilização , Rizoma/química , Solventes/química , Tensoativos/química , Água/química
10.
Food Chem ; 336: 127660, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32771898

RESUMO

Curcumin is a powerful coloring agent widely used in the food industry. Its extraction from the plant Curcuma longa is commonly done with aqueous solvent solutions. In contrast to the conventional extraction methods, the present study aimed to compare two different green and bio-based surfactant-free microemulsion (SFME) extraction systems, which are approved for food and yield a higher extracting power of curcuminoids. Two SFMEs, water/ethanol/triacetin and water/diacetin/triacetin, were investigated via dynamic light scattering. Curcumin solubility in binary mixtures consisting of ethanol/triacetin or diacetin/triacetin was studied both experimentally and theoretically using UV-Vis measurements and COSMO-RS. The SFMEs were further examined and compared to a common ethanol/water (80/20) extraction mixture with respect to their extracting ability using high performance liquid chromatography. The SFMEs containing ethanol were found to extract ~18% more curcuminoids than the SFMEs containing diacetin and ~53% more than the ordinary ethanol/water mixture.


Assuntos
Curcuma/química , Curcumina/química , Curcumina/isolamento & purificação , Emulsões/química , Cromatografia Líquida de Alta Pressão/métodos , Curcumina/análise , Diarileptanoides/química , Difusão Dinâmica da Luz , Etanol/química , Química Verde , Extratos Vegetais/química , Solubilidade , Solventes/química , Espectrofotometria Ultravioleta , Tensoativos/química , Triacetina/química , Água/química
11.
Food Chem ; 338: 127794, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32798827

RESUMO

In this research, a three-step strategy was utilized for discriminating turmeric samples from different provinces and regions in China. Firstly, MRM-based UPLC-MS/MS method for chemical profiling of curcuminoids in turmeric samples was established. Then, response surface methodology was applied for optimizing the extraction process of targeted curcuminoids. Finally, multivariate analysis was conducted for systematic characterization of 66 curcuminoids in turmeric. Principal component analysis (PCA) and orthogonal projection to latent structure-discriminant analysis (OPLS-DA) revealed that turmeric samples from Sichuan and other regions could be classified into two distinct groups. Turmeric samples from the same group had similar curcuminoids content distribution. 25 differential curcuminoids were discovered through OPLS-DA, among which most curcuminoids were more abundant in Sichuan. Furthermore, turmeric samples from different provinces could be clearly discriminated based on hierarchical cluster analysis (HCA) using the screened differential curcuminoids.


Assuntos
Cromatografia Líquida de Alta Pressão , Curcuma/química , Diarileptanoides/química , Espectrometria de Massas em Tandem , China , Análise por Conglomerados , Curcuma/classificação , Análise Discriminante , Análise Multivariada , Análise de Componente Principal
12.
Int J Mol Sci ; 21(24)2020 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-33322413

RESUMO

Current standard-of-care treatment for glioblastoma, the most common malignant primary central nervous system (CNS) tumor, consists of surgical resection followed by adjuvant chemotherapy and radiation (Stupp protocol), providing an overall median survival of 15 months. With additional treatment using tumor-treating fields (Optune® therapy, Novocure Ltd., Haifa, Israel), survival can be extended up to 20 months. In spite of significant progress in our understanding of the molecular pathogenesis, the prognosis for patients with malignant gliomas remains poor and additional treatment modalities are critically needed. Curcumin is a bright yellow pigment found in the rhizome of the widely utilized spice, turmeric (Curcuma longa). It has long been used in South Asian traditional medicines and has been demonstrated to have in vitro antioxidant, anti-inflammatory, and antiproliferative effects. Curcumin has been demonstrated to induce multiple cytotoxic effects in tumor cells including cell cycle arrest, apoptosis, autophagy, changes in gene expression, and disruption of molecular signaling. Additionally, curcumin has been shown to potentiate the effect of radiation on cancer cells, while exhibiting a protective effect on normal tissue. Curcumin's positive safety profile and widespread availability make it a promising compound for future clinical trials for high-grade gliomas.


Assuntos
Antineoplásicos/uso terapêutico , Curcumina/uso terapêutico , Glioblastoma/tratamento farmacológico , Animais , Anti-Inflamatórios/uso terapêutico , Curcuma , Humanos , Extratos Vegetais/uso terapêutico
13.
Nutrients ; 12(10)2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33053656

RESUMO

The severe acute respiratory syndrome coronavirus (SARS-CoV)-2 disease (COVID)-19 is having profound effects on the global economy and food trade. Limited data are available on how this pandemic is affecting our dietary and lifestyle-related behaviors at the global level. Google Trends was used to obtain worldwide relative search volumes (RSVs) covering a timeframe from before the COVID-19 pandemic 1 June 2019 to 27 April 2020. Spearman's rank-order correlation coefficients were used to measure relationships between daily confirmed cases and aforementioned RSVs between 31 December 2019 and 15 April 2020. RSV curves showed increased interest in multiple keywords related to dietary and lifestyle behaviors during the COVID-19 lockdown period in March and April 2020. Spearman's correlation analysis showed that the strongest variables in each keyword category were (1) food security (food shortage: r = 0.749, food bank: r = 0.660, and free food: r = 0.555; all p < 0.001), (2) dietary behaviors (delivery: r = 0.780, restaurant: r = -0.731, take-away: r = 0.731, and food-delivery: r = 0.693; all p < 0.001), (3) outdoor-related behaviors (resort: r = -0.922, hotel: r = -0.913, cinema: r = -0.844, park: r = -0.827, fitness: r = -0.817, gym: r = -0.811; plant: r = 0.749, sunbathing: r = 0.668, and online: r = 0.670; all p < 0.001), and (4) immune-related nutrients/herbs/foods (vitamin C: r = 0.802, vitamin A: r = 0.780, zinc: r = 0.781, immune: r = 0.739, vitamin E: r = 0.707, garlic: r = 0.667, omega-3 fatty acid: r = -0.633, vitamin D: r = 0.549, and turmeric: r = 0.545; all p < 0.001). Restricted movement has affected peoples' dietary and lifestyle behaviors as people tend to search for immune-boosting nutrients/herbs and have replaced outdoor activities with sedentary indoor behaviors.


Assuntos
Infecções por Coronavirus , Dieta , Comportamento Alimentar , Abastecimento de Alimentos , Estilo de Vida , Pandemias , Pneumonia Viral , Isolamento Social , Betacoronavirus , Coronavirus , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Curcuma , Ácidos Graxos Ômega-3 , Alho , Comportamentos Relacionados com a Saúde , Humanos , Comportamento de Busca de Informação , Nutrientes , Pneumonia Viral/epidemiologia , Pneumonia Viral/imunologia , Pneumonia Viral/virologia , Restaurantes , Ferramenta de Busca , Comportamento Sedentário , Síndrome Respiratória Aguda Grave , Oligoelementos , Vitaminas
14.
Arq. Ciênc. Vet. Zool. UNIPAR (Online) ; 23(2, cont.): e2311, jul-dez. 2020. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1141381

RESUMO

Currently, a large number of consumers seek to include functional foods in their diets, aiming beyond the nutritional value prevent health problems, among these foods are probiotic products and vegetables containing bioactive compounds. The objective of this work was to develop and physical-chemically evaluate fresh Shanklish cheeses with the addition of kefir and turmeric extract in order to develop a functional cheese. Shanklish cheese was manufactured and submitted to three different treatments: with the addition of kefir, turmeric extract and both of them. The results obtained for cheese composition were close to the results found in the literature and the cheeses showed stable pH values during the 21 days of storage at 8ºC. It concluded that the addition of kefir and turmeric extract in Shanklish cheese is a way to develop a functional cheese.(AU)


Atualmente é grande o número de consumidores que buscam incluir alimentos funcionais em suas dietas visando além do valor nutricional a prevenção de problemas de saúde, entre esses alimentos encontram-se os produtos probióticos e os vegetais contendo compostos bioativos. O objetivo deste trabalho foi desenvolver e avaliar físico-quimicamente queijos Chancliche frescos com adição de kefir e extrato de cúrcuma buscando o desenvolvimento de um queijo funcional. Foram fabricados queijos Chancliche e submetidos a três tratamentos diferentes: com adição de kefir, extrato de cúrcuma e ambos. Os resultados obtidos para a composição dos queijos estavam próximos dos resultados encontrados na literatura e os queijos mostraram pH estável durante os 21 dias de armazenamento a 8ºC. Concluiu-se que a adição de kefir e extrato de cúrcuma em queijo Chancliche é uma maneira de desenvolver um queijo funcional.(AU)


Actualmente, una gran cantidad de consumidores buscan incluir alimentos funcionales en sus dietas, apuntando más allá del valor nutricional para prevenir problemas de salud, entre estos alimentos se encuentran los productos probióticos y los vegetales que contienen compuestos bioactivos. El objetivo de este trabajo fue desarrollar y evaluar químicamente quesos Chancliche frescos con la adición de kéfir y extracto de cúrcuma para desarrollar un queso funcional. Los quesos Chancliche fueron elaborados y sometidos a tres tratamientos diferentes: con la adición de kéfir, extracto de cúrcuma y ambos. Los resultados obtenidos para la composición de los quesos estaban cercanos a los resultados encontrados en la literatura y los quesos mostraron pH estable durante los 21 días de almacenamiento a 8ºC. Se concluyó que la adición de kéfir y extracto de cúrcuma en queso Chancliche es una forma de desarrollar un queso funcional.(AU)


Assuntos
Queijo , Físico-Química , Curcuma , Alimento Funcional , Kefir , Valor Nutritivo , Probióticos
15.
Zhongguo Zhong Yao Za Zhi ; 45(16): 3863-3870, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893582

RESUMO

This study aimed to establish a rapid and accurate method for identification of raw and vinegar-processed rhizomes of Curcuma kwangsiensis, in order to predict the content of curcumin compounds for scientific evaluation. A complete set of bionics recognition mode was adopted. The digital odor signal of raw and vinegar-processed rhizomes of Curcuma kwangsiensis were obtained by e-nose, and analyzed by back propagation(BP) neural network algorithm, with the accuracy, the sensitivity and specificity in discriminant model, correlation coefficient as well as the mean square error in regression model as the evaluation indexes. The experimental results showed that the three indexes of the e-nose signal discrimination model established by the neural network algorithm were 100% in training set, correction set and prediction set, which were obviously better than the traditional decision tree, naive bayes, support vector machine, K nearest neighbor and boost classification, and could accurately differentiate the raw and vinegar products. Correlation coefficient and mean square error of the regression model in prediction set were 0.974 8 and 0.117 5 respectively, and could well predict curcumin compounds content in Curcuma kwangsiensis, and demonstrate the superiority of the simulation biometrics model in the analysis of traditional Chinese medicine. By BP neural network algorithm, e-nose odor fingerprint could quickly, conveniently and accurately realize the discrimination and regression, which suggested that more bionics information acquisition and identification patterns could be combined in the field of traditional Chinese medicine, so as to provide ideas and methods for the rapid evaluation and stan-dardization of the quality of traditional Chinese medicine.


Assuntos
Curcumina , Nariz Eletrônico , Ácido Acético , Teorema de Bayes , Curcuma , Redes Neurais de Computação , Rizoma
16.
Zhongguo Zhong Yao Za Zhi ; 45(13): 3079-3084, 2020 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-32726014

RESUMO

The systematic breeding method was adopted to breed a new good cultivar of Curcuma longa, named "Chuanjianghuang 1". From 2014 to 2015, two consecutive years of multi-point test were carried out in Shuangliu, Chongzhou and Wenjiang. The biological characters, phenology, agronomic characters, yield and quality indexes of "Chuanjianghuang 1" were comprehensively evaluated. The results showed that compared with local traditional species, the rhizome yield of the new cultivar "Chuanjianghuang 1" increased by 20.61%.The average content of volatile oil was higher than 24.17% and the average content of curcumin in root tuber was higher than 26.62%. The yield of root tuber increased by 54.59%.The average content of volatile oil is higher than 36.28% and the average content of curcuminoids is higher than 25.31%. Compared with "Huangsi Yujin 1", "Chuanjianghuang 1" increased the average yield of rhizome by 123.68%,the volatile oil increased by an average of 7.69%and the curcumin content increased by an average of 58.23%. The average content of volatile oil is higher than 52.82% and the average content of curcuminoids in root tuber was higher than 38.34%. The new variety "Chuanjianghuang 1" has better yield than the local traditional species, and the internal quality of rhizome and root tuber is better. Compared with "Huangsi Yujin 1", the yield of rhizome is significantly increased, and the internal quality of rhizome and root tuber is better, especially the content of curcumin in rhizome and curcuminoids in root tuber is significantly higher than that of "Huangsi Yujin 1". "Chuanjianghuang 1" is high yield, good quality, good stability and strong adaptability, which is suitable for cultivation and promotion in Chengdu Jinma River Basin, such as Shuangliu, Chongzhou, Wenjiang.


Assuntos
Diarileptanoides , Óleos Voláteis , Cruzamento , Curcuma , Rizoma
17.
Medicine (Baltimore) ; 99(28): e20373, 2020 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-32664057

RESUMO

BACKGROUND: Acetaminophen (paracetamol) is one of the most commonly used over-the-counter for pain relief. Management of acute pain with plant-based nutrients has remained suboptimal due to an absence of data supporting acute relief of pain. In the present study, it was hypothesized that high-dissolution liquid treatment of black sesame extract oil, Curcuma longa and Boswellia serrata may provide pain relief in people with acute musculoskeletal pain as quickly as acetaminophen. METHODS: In this randomized active controlled open label study, 88 healthy subjects with acute musculoskeletal pain were randomized to receive treatment capsule (Rhuleave-K; 1,000 mg/d) or 1,000 mg/d acetaminophen for 7 days. Change in pain intensity and pain relief at first 6 hours, 3 days, and 7 days were measured. The onset of analgesia was measured by perceptible pain relief and meaningful pain relief. Other measures were McGill Pain Questionnaire and Patient Global Impression Change. RESULTS: The treatment formulation resulted in average magnitude of pain relief comparable to the acetaminophen. Sixty-six percent of subjects in the treatment group reported positive response in pain relief (≥50% max TOTPAR; total pain relief) after 6 hours, compared to 73% of control. Seventy-three percent of subjects on treatment were considered positive responders, compared to 80% in the control group. The average time of onset of analgesia was 1 hour for the treatment group, versus 0.83 hour for control. At the end of day 3 and 7, there was significant improvement (P < .001 for day 3 and day 7) in the pain condition of treatment group and was comparable to control (P = .436 for day 3 and P = .529 for day 7). The total McGill Pain score showed significant reduction in pain with the treatment irrespective of the pain intensity statistically equal (P = .468) to control. Both the groups were equal in providing sensory pain relief (P = .942), but the treatment was 8.57 times significantly better (P = .027) than acetaminophen in reducing the unpleasantness and emotional aspects (affective domain) involved with acute pain. CONCLUSION: The results showed that the treatment used in the study may act as a natural, fast acting, and safe alternative for acute pain relief comparable to acetaminophen.


Assuntos
Antioxidantes/uso terapêutico , Dor Musculoesquelética/tratamento farmacológico , Fitoterapia , Extratos Vegetais/uso terapêutico , Óleo de Gergelim/uso terapêutico , Adulto , Boswellia , Curcuma , Humanos , Pessoa de Meia-Idade , Sesamum , Resultado do Tratamento
18.
Life Sci ; 257: 117658, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32621921

RESUMO

BACKGROUND: Curcumin (Cur) is a hydrophobic polyphenol compound derived from the rhizome of the herb Curcuma longa. Cur has a wide spectrum of biological and pharmacological activities. It has been shown that human cytomegalovirus (HCMV) infection was an important risk factor for atherosclerosis (AS) and Cur exhibited an outstanding anti-HCMV effect. However, anti-AS effects of Cur remain unclear when HCMV infected endothelial cells. AIMS: This study will investigate the anti-AS activities and mechanism of Cur,when HCMV infected in vivo and in vitro. MATERIALS AND METHODS: Cur (0.5, 1, and 2 µM) was used to explore the anti-AS activities and mechanism after HCMV infected endothelial cells in vitro. ApoE-/- mice were fed a high fat and cholesterol diet (HD) and given 4000,000 copies/mouse MCMV infection by intraperitoneal and treated with ganciclovir (5 mg/kg/d), Cur (25, 15 mg/kg/d) for 10 weeks in vivo. KEY FINDINGS: As our results showed that Cur inhibited CMV replication and proliferation, reduced the intracellular ROS overproduction, decreased the release of inflammatory cytokines, down-regulated the level of HMGB1-TLRS-NF-κB signaling pathway-related proteins in vitro experiments. Cur reduced the serum levels of LDL-C, TC and TG, significantly decreased the formation of atherosclerotic plaque in the aorta, reduced the lipid deposition in liver and inflammatory damage in heart, lung and kidney in vivo experiments. SIGNIFICANCE: This study showed that Cur prevent AS progression by inhibiting CMV activity and CMV-induced HMGB1-TLRS-NF-κB signaling pathway.


Assuntos
Aterosclerose/tratamento farmacológico , Curcumina/farmacologia , Citomegalovirus/efeitos dos fármacos , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Apolipoproteínas E/genética , Apolipoproteínas E/metabolismo , Aterosclerose/metabolismo , Curcuma/metabolismo , Curcumina/metabolismo , Citocinas/metabolismo , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Células Endoteliais da Veia Umbilical Humana , Humanos , Masculino , Camundongos , Camundongos Transgênicos , NF-kappa B/metabolismo , Extratos Vegetais/farmacologia , Placa Aterosclerótica/metabolismo , Transdução de Sinais/efeitos dos fármacos
19.
Arch Microbiol ; 202(8): 2269-2277, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32535791

RESUMO

Finding an effective alternative way to aid defense cells to fight Streptococcus mutans was the main goal of this study. The effect of plant extracts from Curcuma longa L. (turmeric), Rosmarinus officinalis L. (rosemary), and Thymus vulgaris L. (thyme) was evaluated on murine macrophages (RAW 264.7) infected by S. mutans. Minimum inhibitory concentration (MIC) of the extracts was determined. Macrophages were infected by S. mutans and treated with each extract. From the supernatants, it was measured nitric oxide (NO) level. Posteriorly, RAW 264.7 were lysed to expose living and phagocytosed bacteria. Cytotoxicity was checked by lysosomal activity analysis, using neutral red assay. Each extract helped RAW 264.7 to eliminate S. mutans during infection, as observed by a significant bacterial reduction. Significant cell viability was also found. Besides, an increased production of NO was verified using R. officinalis L. and T. vulgaris L. extracts. The evaluated extracts demonstrated an effective action to assist RAW 264.7 to fight S. mutans during infection.


Assuntos
Macrófagos/efeitos dos fármacos , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Infecções Estreptocócicas/tratamento farmacológico , Streptococcus mutans , Animais , Curcuma/química , Camundongos , Testes de Sensibilidade Microbiana , Fagocitose/efeitos dos fármacos , Rosmarinus/química , Thymus (Planta)/química
20.
Artigo em Inglês | MEDLINE | ID: mdl-32521782

RESUMO

The circadian rhythm of biological systems is an important consideration in developing health interventions. The immune and oxidative defense systems exhibit circadian periodicity, with an anticipatory increase in activity coincident with the onset of the active period. Spice consumption is associated with enhanced oxidative defense. The objective of this study was to test the feasibility of a protocol, comparing the effects of morning vs. evening consumption of turmeric on urine markers of oxidative stress in obese, middle-aged adults. Using a within-sample design, participants received each of four clock time x treatment administrations, each separated by one week: morning turmeric; evening turmeric; morning control; evening control. Participants prepared for each lab visit by consuming a low-antioxidant diet for two days and fasting for 12 h. Urine was collected in the lab at baseline and one-hour post-meal and at home for the following five hours. The results showed that the processes were successful in executing the protocol and collecting the measurements and that participants understood and adhered to the instructions. The findings also revealed that the spice treatment did not elicit the expected antioxidant effect and that the six-hour post-treatment urine collection period did not detect differences in urine endpoints across treatments. This feasibility study revealed that modifications to the spice treatment and urine sampling timeline are needed before implementing a larger study.


Assuntos
Curcuma , Obesidade , Estresse Oxidativo/fisiologia , Adulto , Biomarcadores/urina , Ritmo Circadiano , Estudos de Viabilidade , Humanos , Pessoa de Meia-Idade
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