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1.
Food Chem ; 355: 129624, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-33799268

RESUMO

A choline chloride + lactic acid (1:1) natural deep eutectic solvent (NADES) is used as an adjuvant to ethanol/triacetin mixtures to solubilize and extract curcumin from Curcuma Longa. The obtained NADES/ethanol/triacetin mixtures are homogeneous, transparent and of low viscosity even in the absence of water. Dynamic light scattering revealed significant nanostructures, typical of surfactant-free microemulsions. A twofold increase of curcumin solubility and remarkable extraction power (yield of ~90%) can be achieved in the ternary system including the NADES, although curcumin is hydrophobic and the used NADES are very polar. Due to the elevated solubility of curcumin, more extraction cycles can be made than in the previously published aqueous systems with the same amount of solution. As a result, less solvent is required to achieve the same extraction yield.


Assuntos
Curcuma/química , Curcumina/química , Extratos Vegetais/química , Difusão Dinâmica da Luz , Interações Hidrofóbicas e Hidrofílicas , Solubilidade , Solventes/química , Tensoativos/química , Viscosidade
2.
Molecules ; 26(6)2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33802053

RESUMO

Curcuma root (Curcuma longa L.) is a very important plant in gastronomy and medicine for its unique antiseptic, anti-inflammatory, antimicrobial and antioxidant properties. Conventional methods for the extraction of curcuma oil require long extraction times and high temperatures that can degrade the active substances. Therefore, the objectives of the present study were: (i) first, to optimize the extraction yield of curcuma oil by applying a Box-Behnken experimental design using surface response methodology to the microwave-assisted extraction (MAE) technique (the independent variables studied were reaction time (10-30 min), microwave power (150-200 W) and curcuma powder/ethanol ratio (1:5-1:20; w/v); and, (ii) second, to assess the total phenolic content (TPC) and their antioxidant activity of the oil (at the optimum conditions point) and compare with the conventional Soxhlet technique. The optimum conditions for the MAE were found to be 29.99 min, 160 W and 1:20 w/v to obtain an optimum yield of 10.32%. Interestingly, the oil extracted by microwave-assisted extraction showed higher TPC and better antioxidant properties than the oil extracted with conventional Soxhlet technique. Thus, it was demonstrated that the method applied for extraction influences the final properties of the extracted Curcuma longa L. oil.


Assuntos
Curcuma/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Anti-Inflamatórios , Antioxidantes , Etanol/química , Micro-Ondas , Fenóis
3.
Molecules ; 26(5)2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33652694

RESUMO

Colorectal cancer (CRC) is the third most common type of cancer worldwide and a leading cause of cancer death. According to the Malaysian National Cancer Registry Report 2012-2016, colorectal cancer was the second most common cancer in Malaysia after breast cancer. Recent treatments for colon cancer cases have caused side effects and recurrence in patients. One of the alternative ways to fight cancer is by using natural products. Curcumin is a compound of the rhizomes of Curcuma longa that possesses a broad range of pharmacological activities. Curcumin has been studied for decades but due to its low bioavailability, its usage as a therapeutic agent has been compromised. This has led to the development of a chemically synthesized curcuminoid analogue, (2E,6E)-2,6-bis(2,3-dimethoxybenzylidine) cyclohexanone (DMCH), to overcome the drawbacks. This study aims to examine the potential of DMCH for cytotoxicity, apoptosis induction, and activation of apoptosis-related proteins on the colon cancer cell lines HT29 and SW620. The cytotoxic activity of DMCH was evaluated using the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) cell viability assay on both of the cell lines, HT29 and SW620. To determine the mode of cell death, an acridine orange/propidium iodide (AO/PI) assay was conducted, followed by Annexin V/FITC, cell cycle analysis, and JC-1 assay using a flow cytometer. A proteome profiler angiogenesis assay was conducted to determine the protein expression. The inhibitory concentration (IC50) of DMCH in SW620 and HT29 was 7.50 ± 1.19 and 9.80 ± 0.55 µg/mL, respectively. The treated cells displayed morphological features characteristic of apoptosis. The flow cytometry analysis confirmed that DMCH induced apoptosis as shown by an increase in the sub-G0/G1 population and an increase in the early apoptosis and late apoptosis populations compared with untreated cells. A higher number of apoptotic cells were observed on treated SW620 cells as compared to HT29 cells. Human apoptosis proteome profiler analysis revealed upregulation of Bax and Bad proteins and downregulation of Livin proteins in both the HT29 and SW620 cell lines. Collectively, DMCH induced cell death via apoptosis, and the effect was more pronounced on SW620 metastatic colon cancer cells, suggesting its potential effects as an antimetastatic agent targeting colon cancer cells.


Assuntos
Antineoplásicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Neoplasias do Colo/tratamento farmacológico , Curcumina/farmacologia , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Proteínas Reguladoras de Apoptose/genética , Sobrevivência Celular/efeitos dos fármacos , Neoplasias do Colo/patologia , Curcuma/química , Curcumina/análogos & derivados , Curcumina/química , Diarileptanoides/química , Diarileptanoides/farmacologia , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Células HT29 , Humanos , Extratos Vegetais/química , Extratos Vegetais/farmacologia
4.
Molecules ; 26(3)2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-33573219

RESUMO

Bis(demethoxy)curcumin (BDMC) is one of the main active components found in turmeric. Major drawbacks for its usage are its low aqueous solubility, and the challenging separation from other curcuminoids present in turmeric. Co-crystallization can be applied to alter the physicochemical properties of BDMC in a desired manner. A co-crystal screening of BDMC with four hydroxybenzenes was carried out using four different methods of co-crystal production: crystallization from solution by slow solvent evaporation (SSE), and rapid solvent removal (RSR), liquid-assisted grinding (LAG), and crystallization from the melt phase. Two co-crystal phases of BDMC were obtained with pyrogallol (PYR), and hydroxyquinol (HYQ). PYR-BDMC co-crystals can be obtained only from the melt, while HYQ-BDMC co-crystals could also be produced by LAG. Both co-crystals possess an equimolar composition and reveal an incongruent melting behavior. Infrared spectroscopy demonstrated the presence of BDMC in the diketo form in the PYR co-crystals, while it is in a more stable keto-enol form in the HYQ co-crystals. Solubility measurements in ethanol and an ethanol-water mixture revealed an increase of solubility in the latter, but a slightly negative effect on ethanol solubility. These results are useful for a prospective development of crystallization-based separation processes of chemical similar substances through co-crystallization.


Assuntos
Curcuma/química , Curcumina/química , Diarileptanoides/química , Pirogalol/química , Cristalização , Curcumina/síntese química , Diarileptanoides/síntese química , Etanol , Pirogalol/síntese química , Técnicas de Síntese em Fase Sólida , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Água
5.
Ecotoxicol Environ Saf ; 208: 111739, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396067

RESUMO

Ultraviolet-B is an important fraction of sunlight which influences the plant performance either positively or adversely in terms of growth, physiology, biochemistry, and major active compounds. The static nature of plants constrains them to be subjected to various adverse environmental conditions. Several studies performed with plants and UV-B with fewer reports are available on medicinal plants having rhizome. The present study focuses on transformation induced in two Curcuma spp. (C. caesia and C. longa) under the influence of elevated UV-B (eUV-B) (ambient ±9.6 kJ m-2 d-1) under natural field conditions to analyse the changes in physiological, biochemical and essential oil of the test plants. eUV-B significantly reduced the photosynthetic activities such as photosynthetic rate (Ps), stomatal conductance (gs), transpiration (Tr), internal CO2 (Ci), and photochemical efficiency (Fv/Fm) with higher reductions in C. longa as compared to C. caesia. The enzymatic activities of PAL, CHI, and CAD showed higher stimulation in C. caesia whereas C. longa showed increment only in CAD. The essential oil content was increased by 16% and 9% in C. caesia and C. longa, respectively. C. caesia showed increased monoterpenes than sesquiterpenes, whereas almost equal increase of both the terpenoid found in C. longa. C. caesia showed induction of aromatic compounds (epiglobulol, germacrene, 4-terpineol), whereas anticancerous compounds; aphla-terpinolene (61%), beta-caryophyllene (60%), and beta-sesquiphellandrene (32%) were increased in C. longa. C. caesia acted well in terms of both physiology and major active compound (1, 8-cineole), but overall most of the compounds increased in C. longa under eUV-B.


Assuntos
Curcuma/efeitos da radiação , Raios Ultravioleta , Curcuma/química , Curcuma/fisiologia , Óleos Voláteis/farmacologia , Fotossíntese/efeitos dos fármacos , Extratos Vegetais , Plantas/efeitos dos fármacos , Rizoma/química , Terpenos
6.
Carbohydr Polym ; 254: 117401, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33357890

RESUMO

Low bioavailability and poor water solubility have limited the utilization of curcumin in conventional dosing methods. As an alternative, microemulsions as drug carrier can improve curcumin delivery. A cetyltrimethylammonium bromide-nanocrystalline cellulose (CTAB-NCC)-based microemulsion was developed and its potential use as a topical delivery method for curcumin was investigated. The effect of microemulsion's particle size and its microstructure as well as the presence of the CTAB-NCC nanoparticle on the topical delivery of curcumin was studied. In vitro permeation studies showed higher penetration rate of curcumin from the oil-in-water type-microemulsions. The skin permeation profile of curcumin followed Higuchi release kinetics. Furthermore, use of the (CTAB-NCC)-based microemulsion enhanced curcumin accumulation in the skin and these system showed non cytotoxicity effect on L929 cell line. These results showed the potential of (CTAB-NCC)-based microemulsions as controlled-release topical systems for the delivery of curcumin and potentially other lipophilic drugs.


Assuntos
Administração Tópica , Celulose/química , Cetrimônio/química , Curcuma/química , Curcumina/administração & dosagem , Portadores de Fármacos/química , Emulsões/química , Nanopartículas/química , Extratos Vegetais/administração & dosagem , Animais , Disponibilidade Biológica , Linhagem Celular , Curcuma/metabolismo , Curcumina/metabolismo , Liberação Controlada de Fármacos , Feminino , Cinética , Masculino , Camundongos , Nanopartículas/metabolismo , Tamanho da Partícula , Permeabilidade , Extratos Vegetais/metabolismo , Ratos , Ratos Sprague-Dawley , Pele/efeitos dos fármacos , Pele/metabolismo , Solubilidade
7.
Food Chem ; 336: 127660, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32771898

RESUMO

Curcumin is a powerful coloring agent widely used in the food industry. Its extraction from the plant Curcuma longa is commonly done with aqueous solvent solutions. In contrast to the conventional extraction methods, the present study aimed to compare two different green and bio-based surfactant-free microemulsion (SFME) extraction systems, which are approved for food and yield a higher extracting power of curcuminoids. Two SFMEs, water/ethanol/triacetin and water/diacetin/triacetin, were investigated via dynamic light scattering. Curcumin solubility in binary mixtures consisting of ethanol/triacetin or diacetin/triacetin was studied both experimentally and theoretically using UV-Vis measurements and COSMO-RS. The SFMEs were further examined and compared to a common ethanol/water (80/20) extraction mixture with respect to their extracting ability using high performance liquid chromatography. The SFMEs containing ethanol were found to extract ~18% more curcuminoids than the SFMEs containing diacetin and ~53% more than the ordinary ethanol/water mixture.


Assuntos
Curcuma/química , Curcumina/química , Curcumina/isolamento & purificação , Emulsões/química , Cromatografia Líquida de Alta Pressão/métodos , Curcumina/análise , Diarileptanoides/química , Difusão Dinâmica da Luz , Etanol/química , Química Verde , Extratos Vegetais/química , Solubilidade , Solventes/química , Espectrofotometria Ultravioleta , Tensoativos/química , Triacetina/química , Água/química
8.
Food Chem ; 339: 128121, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33152891

RESUMO

We investigated how various drying methods affect the chemical components and microstructure of turmeric. We used freeze drying (FD), hot-air drying (HD) at 50 °C and sun drying (SD). Compared to all dried samples, fresh turmeric had greater amounts of chemical components (curcuminoids, phenolic acids, flavonoids and amino acids) andgreater antioxidantactivity (DPPH and FRAP) except for ABTS.+ values. SD caused a severe decline of three major curcuminoids, resulting from UV and light degradation. Curcumin was decreased the most by SD (72%), followed by HD (61%) and FD (55%), respectively. Curcumin degradation to ferulic acid and vanillin was explored by FTIR analysis. The amino acids were decreased by all drying methods. FD was considered to be most suitable for drying turmeric. Microstructure changes were least after freeze drying, but were still evident compared with fresh turmeric.


Assuntos
Curcuma/química , Diarileptanoides/química , Liofilização/métodos , Aminoácidos/análise , Antioxidantes/análise , Antioxidantes/química , Temperatura Baixa , Curcumina/química , Flavonoides/análise , Fenóis/análise , Luz Solar , Temperatura
9.
Food Chem ; 339: 128140, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33152894

RESUMO

Curcumin was extracted from Curcuma Longa employing a green, bio-based, and food-agreed surfactant-free microemulsion (SFME) consisting of water, ethanol, and triacetin. Concerning the high solubility of curcumin in the examined ternary mixtures, it was attempted to produce highly concentrated tinctures of up to a total of ~130 mg/mL curcuminoids in the solvent by repeatedly extracting fresh rhizomes in the same extraction mixture. The amount of water had a significant influence on the number of cycles that could be performed as well as on the extraction of the different curcuminoids. In addition, the purity of single extracts was enhanced to 94% by investigating several purification steps, e.g. vacuum distillation and lyophilization. Through purification before extraction, the water insoluble curcumin extract could be solubilized indefinitely in an aqueous environment. Additional stability tests showed that solutions of curcumin can be stable up to five months when concealed from natural light.


Assuntos
Curcuma/química , Curcumina/química , Emulsões/química , Extratos Vegetais/química , Curcumina/isolamento & purificação , Diarileptanoides/química , Etanol/química , Liofilização , Rizoma/química , Solventes/química , Tensoativos/química , Água/química
10.
Food Chem ; 338: 127794, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32798827

RESUMO

In this research, a three-step strategy was utilized for discriminating turmeric samples from different provinces and regions in China. Firstly, MRM-based UPLC-MS/MS method for chemical profiling of curcuminoids in turmeric samples was established. Then, response surface methodology was applied for optimizing the extraction process of targeted curcuminoids. Finally, multivariate analysis was conducted for systematic characterization of 66 curcuminoids in turmeric. Principal component analysis (PCA) and orthogonal projection to latent structure-discriminant analysis (OPLS-DA) revealed that turmeric samples from Sichuan and other regions could be classified into two distinct groups. Turmeric samples from the same group had similar curcuminoids content distribution. 25 differential curcuminoids were discovered through OPLS-DA, among which most curcuminoids were more abundant in Sichuan. Furthermore, turmeric samples from different provinces could be clearly discriminated based on hierarchical cluster analysis (HCA) using the screened differential curcuminoids.


Assuntos
Cromatografia Líquida de Alta Pressão , Curcuma/química , Diarileptanoides/química , Espectrometria de Massas em Tandem , China , Análise por Conglomerados , Curcuma/classificação , Análise Discriminante , Análise Multivariada , Análise de Componente Principal
11.
Food Chem ; 345: 128772, 2021 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-33310255

RESUMO

We developed a whey protein admixture of turmeric extract by spray drying (TWPC-SD) and by foam mat drying (TWPC-FMD) and compared its bioactive compounds and nutrients contents. TWPC samples were evaluated for preference and acceptability. Vitamins and carotenoids were determined by high-performance liquid chromatography. Total phenolics, curcumin and antioxidant capacity were determined by spectrophotometry. Centesimal composition was performed according to the Association of Official Analytical Chemists. Chemical elements were determined by inductively coupled plasma optical emission spectrometry. TWPC containing 3.6 mg of curcumin showed good acceptability. Carotenoids and riboflavin were not detected in either TWPC. Vitamin C content was maintained, and antioxidant capacity was increased in both products (p < 0.05). TWPC-SD showed higher total phenolic and curcumin contents compared to TWPC-FMD (p < 0.05). Thus, the TWPC-SD is a good alternative for human consumption since it showed good sensory acceptability and its nutrients and bioactive compounds can contribute to human health.


Assuntos
Misturas Complexas/química , Curcuma/química , Nutrientes/análise , Extratos Vegetais/química , Proteínas do Soro do Leite/química , Antioxidantes/análise , Ácido Ascórbico/análise , Carotenoides/análise , Cromatografia Líquida de Alta Pressão , Dessecação , Humanos , Fenóis/análise , Vitaminas/análise
12.
Arch Microbiol ; 202(8): 2269-2277, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32535791

RESUMO

Finding an effective alternative way to aid defense cells to fight Streptococcus mutans was the main goal of this study. The effect of plant extracts from Curcuma longa L. (turmeric), Rosmarinus officinalis L. (rosemary), and Thymus vulgaris L. (thyme) was evaluated on murine macrophages (RAW 264.7) infected by S. mutans. Minimum inhibitory concentration (MIC) of the extracts was determined. Macrophages were infected by S. mutans and treated with each extract. From the supernatants, it was measured nitric oxide (NO) level. Posteriorly, RAW 264.7 were lysed to expose living and phagocytosed bacteria. Cytotoxicity was checked by lysosomal activity analysis, using neutral red assay. Each extract helped RAW 264.7 to eliminate S. mutans during infection, as observed by a significant bacterial reduction. Significant cell viability was also found. Besides, an increased production of NO was verified using R. officinalis L. and T. vulgaris L. extracts. The evaluated extracts demonstrated an effective action to assist RAW 264.7 to fight S. mutans during infection.


Assuntos
Macrófagos/efeitos dos fármacos , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Infecções Estreptocócicas/tratamento farmacológico , Streptococcus mutans , Animais , Curcuma/química , Camundongos , Testes de Sensibilidade Microbiana , Fagocitose/efeitos dos fármacos , Rosmarinus/química , Thymus (Planta)/química
13.
Food Chem ; 331: 127281, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32559596

RESUMO

Curcuminoids are the major bioactive constituents of turmeric, the application of which are limited by the poor bioavailability. In this study, turmeric was fermented by the Monascus purpureus and Eurotium cristatum fungi to enhance its bioavailability. To explore the variations in the curcuminoids contents in fermented turmeric, a targeted predict-verify strategy was established. For targeted analysis of curcuminoids, a compound library containing all possible curcuminoids based on their structural skeleton was predicted and built for targeted scanning. Then, the MS data were automatically matched with the predicted library to verify the corresponding curcuminoids. As a result, 115 curcuminoids (48 novel compounds and 14 compounds reported in turmeric for the first time) were fully characterized in crude and fermented turmeric. Among these curcuminoids, 31 were newly generated in fermented turmeric. The established predict-verify strategy allows for an efficient and automatic metabolomic analysis to screen for curcuminoids with potentially better bioavailability.


Assuntos
Curcuma/química , Diarileptanoides/metabolismo , Alimentos e Bebidas Fermentados/análise , Metabolômica/métodos , Disponibilidade Biológica , Curcuma/metabolismo , Curcuma/microbiologia , Curcumina/química , Curcumina/farmacocinética , Diarileptanoides/farmacocinética , Eurotium/metabolismo , Fermentação , Espectrometria de Massas , Monascus/metabolismo , Extratos Vegetais/química , Software
14.
Nutrients ; 12(4)2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-32344708

RESUMO

Novel coronaviruses (CoV) have emerged periodically around the world in recent years. The recurrent spreading of CoVs imposes an ongoing threat to global health and the economy. Since no specific therapy for these CoVs is available, any beneficial approach (including nutritional and dietary approach) is worth investigation. Based on recent advances in nutrients and phytonutrients research, a novel combination of vitamin C, curcumin and glycyrrhizic acid (VCG Plus) was developed that has potential against CoV infection. System biology tools were applied to explore the potential of VCG Plus in modulating targets and pathways relevant to immune and inflammation responses. Gene target acquisition, gene ontology and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment were conducted consecutively along with network analysis. The results show that VCG Plus can act on 88 hub targets which are closely connected and associated with immune and inflammatory responses. Specifically, VCG Plus has the potential to regulate innate immune response by acting on NOD-like and Toll-like signaling pathways to promote interferons production, activate and balance T-cells, and regulate the inflammatory response by inhibiting PI3K/AKT, NF-κB and MAPK signaling pathways. All these biological processes and pathways have been well documented in CoV infections studies. Therefore, our findings suggest that VCG Plus may be helpful in regulating immune response to combat CoV infections and inhibit excessive inflammatory responses to prevent the onset of cytokine storm. However, further in vitro and in vivo experiments are warranted to validate the current findings with system biology tools. Our current approach provides a new strategy in predicting formulation rationale when developing new dietary supplements.


Assuntos
Ácido Ascórbico/uso terapêutico , Infecções por Coronavirus/tratamento farmacológico , Curcumina/uso terapêutico , Ácido Glicirrízico/uso terapêutico , Imunidade Inata/efeitos dos fármacos , Inflamação/tratamento farmacológico , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Ácido Ascórbico/farmacologia , Coronavirus , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/metabolismo , Infecções por Coronavirus/virologia , Curcuma/química , Curcumina/farmacologia , Citocinas/metabolismo , Combinação de Medicamentos , Sistemas de Liberação de Medicamentos , Ontologia Genética , Glycyrrhiza/química , Ácido Glicirrízico/farmacologia , Humanos , Interferons/metabolismo , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Transdução de Sinais , Biologia de Sistemas , Linfócitos T/metabolismo , Vitaminas/farmacologia , Vitaminas/uso terapêutico
15.
Yakugaku Zasshi ; 140(4): 471-477, 2020.
Artigo em Japonês | MEDLINE | ID: mdl-32238626

RESUMO

Chronic heart failure is the final stage of such heart diseases as hypertension, cardiomyopathy, and myocardial infarction. Since the incidence of heart failure has increased in recent decades, heart failure is now a major public health problem in developed countries, including Japan. Recently, some studies have demonstrated that natural products, used as nutritional supplements, play an important role in preventing the development of heart failure in animal studies. In our previous study, we showed that curcumin, a natural polyphenol compound derived from Curcuma longa, exhibits therapeutic potency against heart failure. To establish the pharmacological therapeutic value of curcumin in heart failure, we have investigated the translational research of curcumin. This report reviews our basic studies and clinical trials using curcumin therapeutically to prevent heart failure, as well as the possibility of clinical applications of curcumin.


Assuntos
Produtos Biológicos/uso terapêutico , Insuficiência Cardíaca/tratamento farmacológico , Insuficiência Cardíaca/prevenção & controle , Fitoterapia , Polifenóis/farmacologia , Polifenóis/uso terapêutico , Animais , Disponibilidade Biológica , Curcuma/química , Modelos Animais de Doenças , Humanos , Polifenóis/isolamento & purificação , Polifenóis/farmacocinética , Ratos , Pesquisa Médica Translacional
16.
Phytother Res ; 34(8): 2067-2073, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32180294

RESUMO

Osteoarthritis is the most common articular disease that can lead to chronic pain and severe disability. Curcumin-an effective ingredient in turmeric with anti inflammatory property-plays an important role in protecting the joints against destructive factors. Gingerols and piperine, are the effective ingredients of ginger and black pepper, which may potentially enhance and sustain the effect of curcumin in this direction. To determine the effect of cosupplementation with turmeric extract, black pepper, and ginger on prostaglandin E2 (PGE2 ) in patients with chronic knee osteoarthritis, compared with Naproxen. Sixty patients with two different levels of knee osteoarthritis (Grade 2 and 3) were studied. Individuals were randomly assigned to receive daily turmeric extract, ginger, and black pepper together or Naproxen capsule for 4 weeks. PGE2 was evaluated by ELISA method. 24-hr recall was also assessed. All of participants completed the study. PGE2 decreased significantly in both groups (p < .001), but there was no significant differences between groups. The results of this study indicated that intake of the selected herbs twice a day for 4 weeks may improve the PGE2 levels in patients with chronic knee osteoarthritis similar to Naproxen drug.


Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Curcuma/química , Curcumina/química , Gengibre/química , Naproxeno/uso terapêutico , Osteoartrite do Joelho/tratamento farmacológico , Piper nigrum/química , Extratos Vegetais/química , Anti-Inflamatórios não Esteroides/farmacologia , Doença Crônica , Método Duplo-Cego , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Naproxeno/farmacologia
17.
Food Chem ; 320: 126567, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32203830

RESUMO

Deliberate chemical contamination of food powders has become a major food safety concern worldwide. This study used Raman imaging and FT-IR spectroscopy to detect Sudan Red and white turmeric adulteration in turmeric powder. While Sudan Red Raman spectral peaks were identifiable in turmeric-Sudan Red samples, Sudan Red false positive detection was observed in binary Raman images, limiting effective quantitative detection. In addition, white turmeric Raman spectral peaks were unidentifiable in turmeric-white turmeric mixtures. However, IR spectra of turmeric-Sudan Red and turmeric-white turmeric samples provided discrete identifier peaks for both the adulterants. Partial least squares regression models were developed using IR spectra for each mixture type. The models estimated Sudan Red and white turmeric concentrations with correlation coefficients of 0.97 and 0.95, respectively. Priority should be given to developing an IR imaging system and incorporating it with Raman system to simultaneously measure of food samples for detection of adulterants.


Assuntos
Compostos Azo/análise , Curcuma/química , Contaminação de Alimentos/análise , Preparações de Plantas/química , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Análise Espectral Raman/métodos , Pós/química
18.
Biomed Res Int ; 2020: 6892961, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32149121

RESUMO

Inflammatory mediators and inflammatory cells in the inflammatory microenvironment promote the transformation of normal cells to cancer cells in the early stage of cancer, promote the growth and development of cancer cells, and induce tumor immune escape. The monomeric active ingredient ß-elemene is extracted from the traditional Chinese medicine Curcuma wenyujin and has been proven to have good anti-inflammatory and antitumor activities in clinical applications for more than 20 years in China. Recent studies have found that this traditional Chinese medicine plays a vital role in macrophage infiltration and M2 polarization, as well as in regulating immune disorders, and it even regulates the transcription factors NF-κB and STAT3 to alter inflammation, tumorigenesis, and development. In addition, ß-elemene regulates not only different inflammatory factors (such as TNF-α, IFN, TGF-ß, and IL-6/10) but also oxidative stress in vivo and in vitro. The excellent anti-inflammatory and antitumor effects of ß-elemene and its ability to alter the inflammatory microenvironment of tumors have been gradually elaborated. Although the study of monomeric active ingredients in traditional Chinese medicines is insufficient in terms of quality and quantity, the pharmacological effects of more active ingredients of traditional Chinese medicines will be revealed after ß-elemene.


Assuntos
Anti-Inflamatórios/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Sesquiterpenos/farmacologia , Microambiente Tumoral/efeitos dos fármacos , Animais , Carcinogênese/efeitos dos fármacos , Linhagem Celular Tumoral , China , Curcuma/química , Humanos , Doenças do Sistema Imunitário/tratamento farmacológico , Macrófagos/efeitos dos fármacos , Medicina Tradicional Chinesa , NF-kappa B , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase/metabolismo , Estresse Oxidativo , Fator de Transcrição STAT3/efeitos dos fármacos , Sesquiterpenos/química , Fatores de Transcrição/efeitos dos fármacos
19.
Curr Pharm Biotechnol ; 21(11): 1006-1015, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32133961

RESUMO

Curcumin, isolated from the rhizome of Curcuma longa, is one of the most extensively studied phytochemicals. This natural compound has a variety of pharmacological effects including antioxidant, anti-inflammatory, anti-tumor, cardio-protective, hepato-protective and anti-diabetic. Wnt signaling pathway, one of the potential targets of curcumin through upregulation and/or downregulation, plays a significant role in many diseases, even in embryogenesis and development of various organs and systems. In order to exert an anti-tumor activity in the organism, curcumin seems to inhibit the Wnt pathway. The downstream mediators of Wnt signaling pathway such as c-Myc and cyclin D1 are also modified by curcumin. This review demonstrates how curcumin influences the Wnt signaling pathway and is beneficial for the treatment of neurological disorders (Alzheimer's and Parkinson's diseases), cancers (melanoma, lung cancer, breast cancer, colon cancer, endothelial carcinoma, gastric carcinoma and hepatocellular carcinoma) and other diseases, such as diabetes mellitus or bone disorders.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Curcuma/química , Curcumina/farmacologia , Fármacos Neuroprotetores/farmacologia , Via de Sinalização Wnt/efeitos dos fármacos , Doença de Alzheimer/tratamento farmacológico , Doença de Alzheimer/metabolismo , Linhagem Celular Tumoral , Humanos , Neoplasias/tratamento farmacológico , Neoplasias/metabolismo , Fármacos Neuroprotetores/isolamento & purificação , Doença de Parkinson/tratamento farmacológico , Doença de Parkinson/metabolismo
20.
Pharm Biol ; 58(1): 257-264, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32208946

RESUMO

Context: Curcumin, the active component of Curcuma longa L. (Zingiberaceae), exhibits a wide variety of biological activities including vasodilation and anti-inflammation.Objective: The relaxant effect of curcumin in tracheal smooth muscle (TSM) was not examined so far, thus, this study was designed to assess the relaxant effect of curcumin on rat TSM and examine the underlying mechanism(s) responsible for this effect.Materials and methods: TSM was contracted by KCl (60 mM) or methacholine (10 µM), and cumulative concentrations of curcumin (12.5, 25, 50, and 100 mg/mL) or theophylline (0.2, 0.4, 0.6, and 0.8 mM, as positive control) were added to organ bath. The relaxant effect of curcumin was examined in non-incubated or incubated tissues with atropine (1 µM), chlorpheniramine (1 µM), indomethacin (1 µM), and papaverine (100 µM).Results: In non-incubated TSM, curcumin showed significant relaxant effects on KCl-induced contraction in a concentration-dependent manner (p < 0.001 for all concentrations). The relaxant effects of curcumin 12.5, 25, and 50 mg/mL were significantly lower in atropine-incubated tissue compared to non-incubated TSM (p < 0.05 to p < 0.001). A significant difference was observed in EC50 between atropine-incubated (48.10 ± 2.55) and non-incubated (41.65 ± 1.81) tissues (p < 0.05). Theophylline showed a significant relaxant effect on both KCl and methacholine-induced contraction in a concentration-dependent manner (p < 0.001 for all cases).Conclusions: The results indicated a relatively potent relaxant effect of curcumin on TSM, which was less marked than the effect of theophylline. Calcium channel blocking and/or potassium channel opening properties of curcumin may be responsible for TSM relaxation.


Assuntos
Canais de Cálcio/metabolismo , Curcumina/farmacologia , Relaxamento Muscular/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Canais de Potássio/metabolismo , Traqueia/efeitos dos fármacos , Animais , Curcuma/química , Curcumina/isolamento & purificação , Relação Dose-Resposta a Droga , Técnicas In Vitro , Masculino , Músculo Liso/metabolismo , Músculo Liso/fisiopatologia , Ratos Wistar , Traqueia/metabolismo , Traqueia/fisiopatologia
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