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1.
Braz J Med Biol Res ; 52(9): e8224, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31482975

RESUMO

Leishmaniasis is a neglected disease that affects a large part of the world population. Knowing the sand fly fauna of a region is of fundamental importance for guiding health surveillance actions related to the prevention and control of leishmaniasis. A total of 86 specimens of sand flies (60 females and 26 males) were collected. Using the classification proposed by Galati (2003), the following species were identified: Lutzomyia longipalpis (Lutz & Neiva, 1912), Migonemyia migonei (França, 1920), Evandromyia cortelezzi (Brethes, 1923), Ev. sallesi (Galvão & Coutinho, 1939), Nyssomyia whitmani (Atunes & Coutinho, 1939), Psathyromyia lutziana (Costa Lima, 1932), Ev. lenti (Mangabeira, 1938), Brumptomyia sp. (França and Parrot, 1921), and Pressatia sp. (Mangabeira, 1942). Using PCR with internal transcribed spacer target to identify infected sand flies, five Lu. longipalpis females were infected with Leishmania spp. Despite the small number of specimens collected, considerable species diversity was found in the study area.


Assuntos
Insetos Vetores/classificação , Insetos Vetores/parasitologia , Leishmania/isolamento & purificação , Psychodidae/classificação , Psychodidae/parasitologia , Animais , Brasil , DNA Espaçador Ribossômico/genética , Feminino , Leishmania/genética , Leishmaniose/transmissão , Masculino , Reação em Cadeia da Polimerase , RNA de Protozoário/genética
2.
Phytopathology ; 109(11): 1900-1907, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31369362

RESUMO

Floricolous downy mildews (Peronospora, oomycetes) are a small, monophyletic group of mostly inconspicuous plant pathogens that induce symptoms exclusively on flowers. Characterization of this group of pathogens, and information about their biology, is particularly sparse. The recurrent presence of a disease causing flower malformation which, in turn, leads to high production losses of the medicinal herb Matricaria chamomilla in Serbia has enabled continuous experiments focusing on the pathogen and its biology. Peronospora radii was identified as the causal agent of the disease, and morphologically and molecularly characterized. Diseased chamomile flowers showed severe malformations of the disc and ray florets, including phyllody and secondary inflorescence formation, followed by the onset of downy mildew. Phylogeny, based on internal transcribed spacer and cox2, indicates clustering of the Serbian P. radii with other P. radii from chamomile although, in cox2 analyses, they formed a separate subcluster. Evidence pointing to systemic infection was provided through histological and molecular analyses, with related experiments validating the impact of soilborne and blossom infections. This study provides new findings in the biology of P. radii on chamomile, thus enabling the reconstruction of this floricolous Peronospora species' life cycle.


Assuntos
Camomila , Peronospora , Camomila/microbiologia , DNA Espaçador Ribossômico/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Genes Fúngicos/genética , Peronospora/classificação , Peronospora/genética , Peronospora/fisiologia , Filogenia , Doenças das Plantas/microbiologia
3.
Rev Inst Med Trop Sao Paulo ; 61: e37, 2019 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-31411267

RESUMO

Candida glabrata complex includes three species identified through molecular biology methods: C. glabrata sensu stricto , C. nivariensis and C. bracarensis . In Mexico, the phenotypic methods are still used in the diagnosis; therefore, the presence of C. nivariensis and C. bracarensis among clinical isolates is still unknown. The aim of this study was to evaluate the utility of a multiplex PCR for the identification of the C. glabrata species complex. DNA samples from 92 clinical isolates that were previously identified through phenotypic characteristics as C. glabrata were amplified by four oligonucleotides (UNI-5.8S, GLA-f, BRA-f, and NIV-f) that generate amplicons of 397, 293 and 223-bp corresponding to C. glabrata sensu stricto , C. nivariensis , and C. bracarensis , respectively. The amplicon sequences were used to perform a phylogenetic analysis through the Maximum Likelihood method (MEGA6), including strains and reference sequences of species belonging to C. glabrata complex. In addition, recombination and linkage disequilibrium were estimated (DnaSP version 5.0) for C. glabrata sensu stricto isolate s . Eighty-eight isolates generated a 397-bp fragment and only in one isolate a 223-bp amplicon was observed. In the phylogenetic tree, the sequences of 397-bp were grouped with C. glabrata reference sequences , and the sequence of 223-bp was grouped with C. bracarensis reference sequences, corroborating the PCR identification. The number of recombination events for the isolates of C. glabrata sensu stricto was zero, suggesting a clonal population structure. Three isolates that did not amplify any of the expected fragments were identified as Saccharomyces cerevisiae through the sequencing of the D1/D2 domain region within the 28S rDNA gene. The multiplex PCR is a fast, cost-effective and reliable tool that can be used in clinical laboratories to identify C. glabrata complex species.


Assuntos
Candida glabrata/genética , Candidíase/microbiologia , DNA Fúngico/genética , Técnicas de Tipagem Micológica/métodos , Candida glabrata/isolamento & purificação , DNA Ribossômico/genética , DNA Espaçador Ribossômico/genética , Feminino , Humanos , Masculino , México , Reação em Cadeia da Polimerase Multiplex , Filogenia , Análise de Sequência de DNA
4.
Vet Parasitol ; 273: 80-85, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31446257

RESUMO

Visceral leishmaniasis is a zoonotic disease caused by Leishmania infantum for which dogs are the main reservoir. In South America, presence of this disease is expanding along with increasing dispersion of its principal vector, the sand-fly Lutzomyia longipalpis. Feline leishmaniasis is an emerging disease in domestic cats, but epidemiological studies in endemic areas of the Amazon region of Brazil are scarce and the role of cats as reservoirs of L. infantum has been debated. The aim of this study was to investigate L. infantum infection in cats living in the Amazon biome region, using serological and molecular methods. A total of 105 cats were subjected to clinical examination and blood samples were taken for immunofluorescent-antibody (IFAT) serological evaluation, to determine anti-Leishmania antibody titers. Conventional PCR and Sanger's sequencing targeting L. infantum chitinase and Leishmania species ribosomal internal transcribed spacer (ITS-1) encoding genes were performed on conjunctival swabs from these cats. Seropositivity was detected in 32 animals (30.48%), thus confirming that contact between these cats and the parasite was occurring. PCR followed by amplicon sequencing showed that three samples (2.86%) were positive for a chitinase gene and six (5.71%) were positive for the ITS-1 gene. Parasite-positive diagnoses presented a statistically significant association with free access to the streets (p = 0.0111), cohabitation with dogs affected previously by VL (p = 0.0006) and absence of backyard cleaning and garbage collection (p = 0.00003). These results emphasize that cats should be included in epidemiological surveys of leishmaniasis, especially in endemic areas, if not as the reservoir host (unproven), at least as a "sentinel host" that is useful for revealing situations of endemic circulation of L. infantum. Moreover, in these areas, feline leishmaniasis needs to be considered in the differential diagnosis among domestic cats presenting alopecia, rarefied hair, lacerations and ulcerative dermatitis.


Assuntos
Doenças do Gato/diagnóstico , Doenças do Gato/epidemiologia , Leishmania infantum , Leishmaniose Visceral/veterinária , Animais , Anticorpos Antiprotozoários/sangue , Brasil/epidemiologia , Doenças do Gato/parasitologia , Gatos , DNA Espaçador Ribossômico/genética , Cães , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Leishmaniose Visceral/diagnóstico , Leishmaniose Visceral/epidemiologia , Reação em Cadeia da Polimerase , Prevalência , Fatores de Risco
5.
Int J Syst Evol Microbiol ; 69(9): 2823-2827, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31259676

RESUMO

Strains of yeast were isolated under a nitrogen-depleted culture condition from decaying tree bark (strain N-12.1) and from mangrove forest water (strain 1-7W.1) sampled at different locations within a mangrove forest site in Ranong province, Thailand. They were found to be genetically and phenotypically different from any currently recognised yeast species. Phylogenetic analysis of nucleotide sequence of three genes, the internal transcribed spacer region 1 and 2 plus 5.8S ribosomal RNA gene (ITS), D1/D2 domain of the large subunit ribosomal RNA gene (LSU D1/D2) and the small subunit of the ribosomal RNA gene (SSU), revealed that these two strains were related to but distinguished from Heterocephalacriaarrabidensis. Several distinct physiological characteristics of these two strains were detected, namely inability to assimilate glycerol, dl-lactic acid, succinic acid, citric acid, d-gluconic acid, and ability to grow well at 25 °C, which were different from those of H. arrabidensis. Accordingly, the name Heterocephalacria mucosa sp. nov., is proposed to accommodate this novel species. The MycoBank number is MB 828624.


Assuntos
Basidiomycota/classificação , Filogenia , Casca de Planta/microbiologia , Basidiomycota/isolamento & purificação , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , RNA Ribossômico 5,8S , Análise de Sequência de DNA , Tailândia , Áreas Alagadas
6.
Zhongguo Zhong Yao Za Zhi ; 44(10): 2046-2050, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31355559

RESUMO

The endophytic fungi from root,main stem,branch and leaf of Scrophularia ningpoensis were isolated from Zhejiang,whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer( ITS) between r DNAs,the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples,which were classified into 9 orders,13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as Alternaria alternate and ZJ25 was identified as A.gaisen by its morphology and authenticated by ITS( ITS4 and ITS5 regions and the intervening 5. 8 S rDNA region).


Assuntos
Fungos/classificação , Glicosídeos/biossíntese , Glicosídeos Iridoides/metabolismo , Piranos/metabolismo , Scrophularia/microbiologia , China , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Endófitos/classificação , Endófitos/metabolismo , Fungos/metabolismo
7.
Int J Syst Evol Microbiol ; 69(10): 3087-3092, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31329532

RESUMO

Four strains, NYNU 15610, NYNU 15612, NYNU 15613 and NYNU 15615, of a novel ascomycetous yeast were isolated from the gut of Allomyrina dichotoma (Coleoptera: Scarabeidae) collected from two different localities in Henan Province, Central PR China. The four strains shared identical sequences in both of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions. Sequence analyses revealed that this novel species represents a member of the genus Metschnikowia. It differed from its closest known species Metschnikowia zobellii, Metschnikowiaaustralis and Metschnikowia bicuspidata, by 8.4-9.2 % sequence divergence (33-40 nt substitutions and 7-12 gaps over 509 bases) in the D1/D2 sequences. The formation of ascospores was not observed on various sporulation media. In contrast to M. zobellii, M. australis and M. bicuspidata, the novel yeast species was unable to assimilate succinate, ethanol, ethylamine, cadaverine and 10 % NaCl plus 5 % glucose, but was able to grow in vitamin-free medium. The name Metschnikowia baotianmanensis f.a., sp. nov. is proposed to accommodate these strains, with NYNU 15613 as the holotype.


Assuntos
Besouros/microbiologia , Metschnikowia/classificação , Filogenia , Animais , China , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Metschnikowia/isolamento & purificação , Técnicas de Tipagem Micológica , Análise de Sequência de DNA , Esporos Fúngicos
8.
Vet Parasitol ; 271: 76-79, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31303209

RESUMO

The equine Parascaris spp. is large, parasitic nematodes, and predominantly focuses on the intestine of foals and young weanlings. There are two roundworms, Parascaris equorum and Parascaris univalens, recognized among equine hosts. In this study, all fifty-nine Parascaris worms were harvested from three different equine hosts (twenty specimens from Equus zebra, twenty specimens from E. caballus, and nineteen specimens from E. asinus). The ribosomal gene (ITS) and mitochondrial genes (cox1 and nadh1) were amplified to identify and genetically characterize these worms. Analysis of ITS sequences revealed five genotypes among the fifty-nine worms, and the sequence similarity among the worms from E. zebra and E. caballus was at a high level (99.87%), while the one of E. asinus worms showed an apparent difference from the worms either from the E. zebra or from the E. caballus (sequence similarity ranging from 93.04 to 93.42%). Analysis of mitochondrial genes revealed that twenty-one (cox1 gene) and thirteen (nadh1 gene) unique haplotypes were defined among the fifty-nine worms. The shared haplotypes (four cox1 haplotypes and one nadh1 haplotype) only occurred between the worm populations from E. zebra and E. caballus. The cox1 and nadh1 haplotype sequences were respectively applied to construct phylogenetic trees. Although the topologies showed that E. asinus worm population had an obvious boundary with the worm populations of the E. zebra and the E. caballus, however, no noticeable boundary was found within the two later worm populations. Meanwhile, the E. asinus worm population showed an obvious genetic differentiation and an extremely low gene flow (close to zero) with the worm populations from E. zebra and E. caballus, indicating that the genetic characteristics of the worms from the E. asinus have an obvious difference with the one from E. zebra and E. caballus.


Assuntos
Ascaridoidea/classificação , Ascaridoidea/genética , Equidae/parasitologia , Cavalos/parasitologia , Filogenia , Animais , DNA Espaçador Ribossômico/genética , Genes Mitocondriais/genética
9.
Plant Dis ; 103(9): 2237-2245, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31306089

RESUMO

Phakopsora pachyrhizi, the causal agent of soybean rust (SBR), is a global threat to soybean production. Since the discovery of SBR in the continental United States, quantitative polymerase chain reaction assays based on the internal transcribed spacer (ITS) ribosomal DNA locus were established for its rapid detection. However, insufficient data were initially available to test assays against factors that could give rise to misidentification. This study aimed to reevaluate current assays for (i) the potential for false-positive detection caused by nontarget Phakopsora species and (ii) the potential for false-negative detection caused by intraspecific variation within the ITS locus of P. pachyrhizi. A large amount of intraspecific and intragenomic variation in ITS was detected, including the presence of polymorphic ITS copies within single leaf samples and within single rust sori. The diagnostic assays were not affected by polymorphisms in the ITS region; however, current assays are at risk of false positives when screened against other species of Phakopsora. This study raises caveats to the use of multicopy genes (e.g., ITS) in single-gene detection assays and discusses the pitfalls of inferences concerning the aerobiological pathways of disease spread made in the absence of an evaluation of intragenomic ITS heterogeneity.


Assuntos
Variação Genética , Phakopsora pachyrhizi , Doenças das Plantas , DNA Espaçador Ribossômico/genética , Técnicas de Diagnóstico Molecular/normas , Patologia Molecular , Phakopsora pachyrhizi/genética , Doenças das Plantas/microbiologia , Soja/microbiologia , Estados Unidos
10.
Int J Syst Evol Microbiol ; 69(9): 2828-2833, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31274406

RESUMO

Strain SYSU-17, representing a novel acid-tolerant yeast species which can grow at pH 2.0 weakly, was isolated from acid mine drainage collected in a tailing impoundment of the Fankou Lead/Zinc Mine, Guangdong Province, PR China. Phylogenetic analysis of strain SYSU-17 based on the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit ribosomal RNA (LSU rRNA) gene suggested that strain SYSU-17 was a novel species belonging to the genus Spencerozyma (class Microbotryomycetes, subphylum Pucciniomycotina). It differed from the type strain of the closest related species, Spencerozyma crocea CBS 2029T, by 0.7 % sequence divergence (three gaps and one nucleotide substitution out of 594 bp) in the D1/D2 domains of the LSU rRNA gene and 7.6 % sequence divergence (32 gaps and 22 nucleotide substitutions out of 714 bp) in the ITS region. In contrast to the physiological properties of S. crocea, the novel yeast species was unable to assimilate galactose, d-ribose, xylitol, succinate, d-xylose, ethanol, nitrate and nitrite. The name Spencerozyma acididurans sp. nov. is proposed and SYSU-17 is designated as the holotype.


Assuntos
Basidiomycota/classificação , Mineração , Filogenia , Microbiologia da Água , Ácidos , Basidiomycota/isolamento & purificação , China , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Análise de Sequência de DNA
11.
DNA Res ; 26(3): 273-286, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31231763

RESUMO

The intergenic spacer (IGS) of rDNA is frequently built of long blocks of tandem repeats. To estimate the intragenomic variability of such knotty regions, we employed PacBio sequencing of the Cucurbita moschata genome, in which thousands of rDNA copies are distributed across a number of loci. The rRNA coding regions are highly conserved, indicating intensive interlocus homogenization and/or high selection pressure. However, the IGS exhibits high intragenomic structural diversity. Two repeated blocks, R1 (300-1250 bp) and R2 (290-643 bp), account for most of the IGS variation. They exhibit minisatellite-like features built of multiple periodically spaced short GC-rich sequence motifs with the potential to adopt non-canonical DNA conformations, G-quadruplex-folded and left-handed Z-DNA. The mutual arrangement of these motifs can be used to classify IGS variants into five structural families. Subtle polymorphisms exist within each family due to a variable number of repeats, suggesting the coexistence of an enormous number of IGS variants. The substantial length and structural heterogeneity of IGS minisatellites suggests that the tempo of their divergence exceeds the tempo of the homogenization of rDNA arrays. As frequently occurring among plants, we hypothesize that their instability may influence transcription regulation and/or destabilize rDNA units, possibly spreading them across the genome.


Assuntos
Cucurbita/genética , DNA Espaçador Ribossômico/genética , Variação Genética , Repetições Minissatélites , Conformação de Ácido Nucleico , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/metabolismo , Análise de Sequência de DNA
12.
Int J Syst Evol Microbiol ; 69(10): 2992-3000, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31166162

RESUMO

The Arabian Gulf surrounding Qatar is distinct from other marine ecosystems due to its high salinity (35-75 PSU) and extreme water temperature fluctuations (11-40 °C). Furthermore, in the last decade, Qatar has been witnessing an industrial boom as well as extensive infrastructure construction activities. Marine micro-organisms, including fungi, remain largely unexplored in the Arabian Gulf. During a 3 year study, we investigated the diversity of marine fungi in coastal waters around Qatar. As a result, two new Toxicocladosporium species were isolated from the Qatari marine environment. Molecular and phylogenetic analyses of rRNA gene sequences of five loci, namely the internal transcribed spacer 1 and 2 regions and the D1/D2 domains of the large subunit rRNA, actin, RNA polymerase second largest subunit and beta-tubulin genes, were used to confirm the identity of the novel species for which we propose the names Toxicocladosporium aquimarinum sp. nov. and Toxicocladosporium qatarense sp. nov.


Assuntos
Ascomicetos/classificação , Filogenia , Água do Mar/microbiologia , Ascomicetos/isolamento & purificação , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Ecossistema , Técnicas de Tipagem Micológica , Catar , Salinidade , Análise de Sequência de DNA
13.
Int J Syst Evol Microbiol ; 69(10): 3014-3021, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31219416

RESUMO

During an extensive survey of marine fungi in coastal marine environments from Portugal, a collection of Penicillium isolates were obtained from sea water, macroalgae and driftwood. Sixteen distinct Penicillium species were identified with Penicillium terrigenum and Penicillium brevicompactum being the most frequent. A Penicillium species isolated from sea water could not be affiliated to any known species. Phylogenetic analyses based on the ITS region of the rDNA and the beta-tubulin (benA) gene placed it into Penicillium section Ramosa, distinct from all currently known species and with Penicillium tunisiense as its closest relative. Although having similar morphological characteristics, these species differ in micromorphological and molecular characters. Thus, Penicillium lusitanum sp. nov. is proposed as a novel species.


Assuntos
Penicillium/classificação , Filogenia , Água do Mar/microbiologia , Biodiversidade , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Penicillium/isolamento & purificação , Portugal , Alga Marinha/microbiologia , Análise de Sequência de DNA , Tubulina (Proteína)/genética , Madeira/microbiologia
14.
Parasitol Res ; 118(7): 2203-2211, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31154527

RESUMO

Mitochondrial (mt) DNA has been useful in revealing the phylogenetic relationship of eukaryotic organisms including flatworms. Therefore, the use of mitogenomic data for the comparative and phylogenetic purposes is needed for those families of digenetic trematodes for which the mitogenomic data are still missing. Molecular data with sufficiently rich informative characters that can better resolve species identification, discrimination, and membership in different genera is also required for members of some morphologically difficult families of trematodes bearing few autapomorphic characters among its members. Here, the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (rDNA) and the complete mt genome of the trematode Uvitellina sp. (Cyclocoelidae: Haematotrephinae) was determined and annotated. The mt genome of this avian trematode is 14,217 bp in length, containing 36 genes plus a single non-coding region. The ITS rDNA sequences were used for the pairwise sequence comparison of Uvitellina sp. with European cyclocoelid species, and the mitochondrial 12 protein-coding genes (PCGs) and two ribosomal RNA genes were used to evaluate the position of the family within selected trematodes. The ITS rDNA analysis of Uvitellina sp. showed less nucleotide differences with Hyptiasmus oculeus (16.77%) than with other European cyclocoelids (18.63-23.58%). The Bayesian inference (BI) analysis using the 12 mt PCGs and two rRNA genes supported the placement of the family Cyclocoelidae within the superfamily Echinostomatoidea (Plagiorchiida: Echinostmata). The availability of the mt genome sequences of Uvitellina sp. provides a novel resource of molecular markers for phylogenetic studies of Cyclocoelidae and other trematodes.


Assuntos
DNA Mitocondrial/genética , Echinostomatidae/genética , Genoma Mitocondrial/genética , Mitocôndrias/genética , Animais , Sequência de Bases , Teorema de Bayes , Aves/parasitologia , DNA Ribossômico/genética , DNA Espaçador Ribossômico/genética , Echinostomatidae/classificação , Filogenia , Análise de Sequência de DNA
15.
Parasitol Int ; 72: 101939, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31201922

RESUMO

Adult Cryptocotyle lata sp. nov. worms were obtained from experimental studies. In the Russian southern Far East, the life cycle of this parasite is carried out using freshwater snails (Boreoelona ussuriensis), freshwater fish, and birds as the first intermediate, second intermediate, and definitive hosts, respectively. The morphological indices of C. lata sp. nov. are closest to Cryptocotyle concava; however, these two species differ in terms of their sizes of body, oral and ventral suckers, eggs, and the shape of their testes and ovaries. Analysis of the life cycles of the Cryptocotyle representatives suggested that C. concava were at least two cryptic species, one of which circulates using brackish water Hydrobia snails, and the other using freshwater Amnicola snails as the first intermediate hosts. Molecular data (i.e., the 28S gene and the second internal transcribed spacer (ITS2) of rDNA) were used to analyze the phylogenetic relationships of C. lata sp. nov. and other representatives of Opisthorchioidea. The long repeats and secondary structure of the ITS1 region were studied. Representatives of the Opisthorchiidae and several species from Heterophyidae (including the genus Cryptocotyle) were found to have molecular features that suggested that these species belonged to Opisthorchiidae. At the same time, the genetic relatedness of worms, which are united in common clusters on phylogenetic trees, is consistent with the use of the first intermediate hosts from different taxonomic groups in their life cycles; namely, snails of the Truncatelloidea are hosts of trematodes from a cluster with Opisthorchiidae and a number species of the family Heterophyidae, while snails of the Cerithioidea are hosts of worms from a cluster that includes only the Heterophyidae. In addition, the results of genetic studies indicate that Clonorchis sinensis, Metorchis ussuriensis, Metorchis bilis, Metorchis xanthosomus, and Metorchis orientalis should be included in the genus Opisthorchis.


Assuntos
Opisthorchidae/classificação , Filogenia , Caramujos/parasitologia , Animais , DNA de Helmintos/genética , DNA Espaçador Ribossômico/genética , Peixes/parasitologia , Estágios do Ciclo de Vida , Opisthorchidae/anatomia & histologia , Opisthorchidae/isolamento & purificação , RNA Ribossômico 28S/genética , Federação Russa
16.
Int J Syst Evol Microbiol ; 69(9): 2658-2661, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31162007

RESUMO

Three strains of a novel yeast species were isolated from rotting wood in the Xishuangbanna Tropical Rainforest, Yunnan Province, PR China. Sequence analysis of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions showed that the novel species represents a member of the genus Saturnispora. It differed from its closest known species, Saturnispora sekii CBS 10931T, by 1.3 % nucleotide substitutions in the D1/D2 domains and by 2.2 % nucleotide substitutions in the ITS regions, respectively. In contrast to Saturnispora sekii, the novel yeast species was unable to assimilate glycerol, dl-lactate, succinate and citrate, and grow at 37 °C. The name Saturnispora galanensis sp. nov. is proposed to accommodate these strains, with NYNU 1797 as the holotype.


Assuntos
Filogenia , Floresta Úmida , Saccharomycetales/classificação , Madeira/microbiologia , China , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA
17.
Int J Syst Evol Microbiol ; 69(9): 2674-2680, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31166159

RESUMO

Three strains, representing a novel anamorphic and d-xylose-fermenting yeast species, were isolated from moss (ST-302T), seawater (ST-1169) and peat (DMKU-XE12) collected from the southern part of Thailand. The three strains had identical sequences of the D1/D2 regions of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions. Candida flosculorum CBS 10566T and Candida sharkiensis CBS 11368T were the most closely related species with 7.9 % nucleotide substitutions in the D1/D2 regions of the LSU rRNA gene, and 10.3 and 12.6% nucleotide substitutions in the ITS regions, respectively. Phylogenetic analysis based on the concatenated sequences of the ITS and the D1/D2 regions confirmed that the three strains represented a distinct anamorphic species in the Clavispora clade. Therefore, the three strains were described as a novel species, for which we propose the name Candida xylosifermentans sp. nov.


Assuntos
Candida/classificação , Filogenia , Xilose/metabolismo , Briófitas/microbiologia , Candida/isolamento & purificação , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Fermentação , Técnicas de Tipagem Micológica , Água do Mar/microbiologia , Análise de Sequência de DNA , Solo , Microbiologia do Solo , Tailândia
18.
Int J Syst Evol Microbiol ; 69(9): 2775-2780, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31237537

RESUMO

During studies on the yeast communities associated with rotting wood in the Xishuangbanna Tropical Rainforest in PR China, four novel yeast strains were found. Phylogenetic analysis based on the concatenated sequences of the D1/D2 domains of the large subunit rRNA gene and the ITS regions showed that these strains represented two novel species in the Candida albicans/Lodderomyces clade. The novel species, represented by strains NYNU 17948 and NYNU 17981, formed a clade with Candida maltosa and Candida baotianmanensis, with 1-1.8% sequence divergence in the D1/D2 domains and 8.9-10% sequence divergence in the ITS regions. The other novel species, represented by NYNU 17105 and NYNU 17763, is most closely related to Candida blackwelliae with 0.7 % sequence divergence in the D1/D2 domains and 6.9 % sequence divergence in the ITS regions. The two novel species could be distinguished from their closest described species in terms of physiological traits. The two novel species are described as Candida yunnanensis sp. nov. (holotype NYNU 17948) and Candida parablackwelliae sp. nov. (holotype NYNU 17763).


Assuntos
Candida/classificação , Filogenia , Floresta Úmida , Madeira/microbiologia , Candida/isolamento & purificação , China , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Fenótipo , Análise de Sequência de DNA
19.
Vet Parasitol ; 270: 20-24, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31213237

RESUMO

Neosporosis is a common cause of abortion in cattle worldwide but is rare in horses. Here, the first case of histologically, ultrastructurally, immunohistochemically, and molecularly confirmed equine abortion caused by neosporosis is reported. Samples of lung, heart, liver, skeletal muscle, tongue, brain, and the placenta from a female fetus aborted at 280 days of gestation were fixed in formalin and submitted for diagnosis. Histologically, there was disseminated neosporosis with severe lesions in lungs, liver and the heart. Protozoal tachyzoites in all tissues reacted with polyclonal anti-Neospora caninum rabbit antibodies. Transmission electron microscopic observation on lung tissue revealed tachyzoites consistent with Neospora, including many rhoptries. Polymerase-chain reaction (PCR) using primers designed to amplify the rRNA gene internal transcribed spacer 1 (ITS1) of the Sarcocystidae was performed on DNA extracted from fetal tissues. Comparison of the ITS1 amplified from the foal tissue to sequences available in GenBank revealed 100% sequence identity to the ITS1 from three isolates of Neospora hughesi.


Assuntos
Feto Abortado/parasitologia , Aborto Animal/parasitologia , Coccidiose/veterinária , Doenças dos Cavalos/parasitologia , Feto Abortado/ultraestrutura , Animais , Anticorpos Antiprotozoários/metabolismo , Coccidiose/diagnóstico , Coccidiose/parasitologia , DNA Espaçador Ribossômico/genética , Feminino , Doenças dos Cavalos/diagnóstico , Cavalos , Imuno-Histoquímica , Microscopia Eletrônica de Transmissão , Neospora/genética , Neospora/ultraestrutura
20.
Rev Bras Parasitol Vet ; 28(2): 303-305, 2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31215604

RESUMO

Leishmania spp. are important agents of human and animal leishmaniases that have an important impact on public health. In this study, we aimed to detect the circulation of Leishmania spp. in cattle from a visceral leishmaniasis non-endemic area of the state of São Paulo, Brazil. DNA was extracted from blood samples from 100 heifers in the municipality of Pirassununga and was amplified using primers specific for the first internal transcriber spacer (ITS1), to assess the presence of trypanosomatids. The assays revealed that one sample presented bands of between 300 and 350 base pairs. In GenBank, this sample matched 100% with Leishmania infantum (314 base pairs). The results suggest that cattle can be infected by Leishmania infantum in Brazil.


Assuntos
Doenças dos Bovinos/diagnóstico , DNA Espaçador Ribossômico/genética , Leishmania infantum/genética , Leishmaniose Visceral/veterinária , Animais , Bovinos , Leishmaniose Visceral/diagnóstico , Reação em Cadeia da Polimerase/veterinária
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