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1.
Environ Pollut ; 256: 113389, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31685327

RESUMO

The boreoatlantic gonate squid (Gonatus fabricii) represents important prey for top predators-such as marine mammals, seabirds and fish-and is also an efficient predator of crustaceans and fish. Gonatus fabricii is the most abundant cephalopod in the northern Atlantic and Arctic Ocean but the trace element accumulation of this ecologically important species is unknown. In this study, trace element concentrations (Ag, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn) were analysed from the mantle muscle and the digestive gland tissue of juveniles, adult females, and adult males that were captured south of Disko Island off West-Greenland. To assess the feeding habitat and trophic position of this species, stable isotopes of carbon (δ13C) and nitrogen (δ15N) were measured in their muscle tissue. Mercury concentrations were positively correlated with size (mantle length) and trophic position. The Hg/Se ratio was assessed because Se has been suggested to play a protective role against Hg toxicity and showed a molar surplus of Se relative to Hg. Cadmium concentrations in the digestive gland were negatively correlated with size and trophic position (δ15N), which suggested a dietary shift from Cd-rich crustaceans towards Cd-poor fish during ontogeny. This study provides trace element concentration data for G. fabricii from Greenlandic waters, which represents baseline data for a northern cephalopod species. Within West-Greenland waters, G. fabricii appears to be an important vector for the transfer of Cd in the Arctic pelagic food web.


Assuntos
32418 , Decapodiformes/metabolismo , Monitoramento Ambiental/métodos , Metais Pesados/análise , Oligoelementos/análise , Poluentes Químicos da Água/análise , Animais , Regiões Árticas , Oceano Atlântico , Clima Frio , Ecossistema , Feminino , Cadeia Alimentar , Masculino , Metais Pesados/metabolismo , Alimentos Marinhos/análise , Oligoelementos/metabolismo , Poluentes Químicos da Água/metabolismo
2.
Carbohydr Polym ; 228: 115370, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31635728

RESUMO

Nanofiber-based materials have recently gained increasing attention in food packaging, drug delivery, and biomedical applications. In this study, a multi-nanofibers composite film was developed based on bacterial cellulose nanofiber (BCNF)/chitin nanofiber (CNF) hybridization. The nanofibers were responsible for the formation of well-dispersed curcumin (Cur) micro/nanoparticles in the nanocomposite films. The release of Cur from the films were affected by CNF and the sizes of Cur particles formed in situ. The Cur particles reduced tensile strength and increased water vapor permeability of BCNF film. However, CNF improved the mechanical strength and barrier property of the Cur/BCNF/CNF composite film. Moreover, the multi-nanofibers composite film showed excellent dynamic antioxidant capacity and antibacterial activity, as well as was capable to monitor pH change and trace amount of boric acid. Results of this study suggested that the Cur/BCNF/CNF composite film can be used as a smart and active food packaging material.


Assuntos
Celulose/química , Quitina/química , Nanofibras/química , Animais , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Curcumina/farmacologia , Decapodiformes/metabolismo , Portadores de Fármacos , Liberação Controlada de Fármacos , Escherichia coli/efeitos dos fármacos , Embalagem de Alimentos , Microesferas , Nanocompostos/química , Nanopartículas/química , Staphylococcus aureus/efeitos dos fármacos , Resistência à Tração
3.
Anim Reprod Sci ; 208: 106107, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31405483

RESUMO

The membrane progestin receptor (mPR) family has been characterized in several species, including fish, frogs, rats, and humans. Results of previous studies indicate mPRs mediate the rapid, nongenomic action of progestins. In this study, the full-length cDNA of Sepiella japonica mPR-beta (mPRß) and mPR-gamma (mPRγ) were characterized. Furthermore, sjmPR mRNA relative abundances were assessed for different tissues. There was also determination of the subcellular localization of mPRs, and investigation of the effect of sjmPRs on ovarian development via proximate actions on the brain and ovary of S. japonica. Results of tissue distribution assays indicated mPRß and mPRγ transcripts were present predominantly in the brain and ovary. As ovaries developed, the abundance of SjmPRs mRNA transcripts increased and peaked during the interstitial growth phase (III), followed by a marked decrease afterward in both the brain and ovary. In addition, confocal microscopy evaluations of HEK293 T cells expressing the mPRs-EGFP gene indicated both SjmPRß and SjmPRγ were localized in the plasma membrane of HEK293 T cells. Taken together, these findings indicate S. japonica protein is a membrane progestin receptor capable of inducing ovary maturation in cephalopods.


Assuntos
Decapodiformes/metabolismo , Regulação da Expressão Gênica/fisiologia , RNA Mensageiro/metabolismo , Receptores de Progesterona/metabolismo , Animais , Feminino , Masculino , RNA Mensageiro/genética
4.
J Agric Food Chem ; 67(37): 10470-10480, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31469565

RESUMO

Foodborne nanoparticles (FNPs) produced by roasting have attracted the attention of people, owing to their safety risk to body health. Herein, we reported the formation, physicochemical properties, elemental composition, biodistribution, and binding with human serum albumin (HSA) of FNPs extracted from roast squid. The results showed that the FNP size gradually decreased from 4.1 to 2.3 nm as the roasting temperature changed from 190 to 250 °C. The main component elements of FNPs are carbon, oxygen, and nitrogen, and the carbon and nitrogen contents of FNPs increased with the roasting temperature rising. The surface of FNPs contained hydroxyl, amino, and carboxyl functional groups. The FNPs can emit fluorescence in ultraviolet light and show excitation-dependent emission behavior. Furthermore, it was found that the FNPs derived from roast squid could be accumulated in the stomach, intestine, and brain of BALB/c mice after oral feeding. Static fluorescence quenching of HSA was found by the Stern-Volmer equation and ultraviolet-visible spectrum analysis after interaction with the FNPs. After the addition of FNPs, the α-helix content of HSA decreased and the morphological height of HSA increased, which indicated that the FNPs could cause structural changes in HSA. The atomic force microscopy characterization showed the formation of nanocorona between FNPs and HSA.


Assuntos
Decapodiformes/química , Nanopartículas/química , Nanopartículas/metabolismo , Albumina Sérica Humana/química , Animais , Culinária , Decapodiformes/metabolismo , Fluorescência , Temperatura Alta , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Microscopia de Força Atômica , Tamanho da Partícula , Ligação Proteica , Conformação Proteica , Albumina Sérica Humana/metabolismo , Distribuição Tecidual
5.
Int J Mol Sci ; 20(17)2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31454889

RESUMO

In order to rapidly and efficiently excavate antihypertensive ingredients in Todarodes pacificus, its myosin heavy chain was hydrolyzed in silico and the angiotensin-converting enzyme (ACE) inhibitory peptides were predicted using integrated bioinformatics tools. The results showed the degree of hydrolysis (DH) theoretically achieved 56.8% when digested with papain, ficin, and prolyl endopeptidase (PREP), producing 126 ACE inhibitory peptides. By predicting the toxicity, allergenicity, gastrointestinal stability, and intestinal epithelial permeability, 30 peptides were finally screened, of which 21 had been reported and 9 were new. Moreover, the newly discovered peptides were synthesized to evaluate their in vitro ACE inhibition, showing Ile-Ile-Tyr and Asn-Pro-Pro-Lys had strong effects with a pIC50 of 4.58 and 4.41, respectively. Further, their interaction mechanisms and bonding configurations with ACE were explored by molecular simulation. The preferred conformation of Ile-Ile-Tyr and Asn-Pro-Pro-Lys located in ACE were successfully predicted using the appropriate docking parameters. The molecular dynamics (MD) result indicated that they bound tightly to the active site of ACE by means of coordination with Zn(II) and hydrogen bonding and hydrophobic interaction with the residues in the pockets of S1 and S2, resulting in stable complexes. In summary, this work proposed a strategy for screening and identifying antihypertensive peptides from Todarodes pacificus.


Assuntos
Inibidores da Enzima Conversora de Angiotensina/química , Inibidores da Enzima Conversora de Angiotensina/farmacologia , Decapodiformes/química , Peptídeos/química , Peptídeos/farmacologia , Animais , Decapodiformes/metabolismo , Descoberta de Drogas , Avaliação Pré-Clínica de Medicamentos , Ativação Enzimática/efeitos dos fármacos , Hidrólise , Conformação Molecular , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Cadeias Pesadas de Miosina/química , Cadeias Pesadas de Miosina/metabolismo , Relação Quantitativa Estrutura-Atividade
6.
Food Chem ; 297: 124941, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31253326

RESUMO

The effect of a hydroxyl radical generating system (HRGS), which contained FeCl3, sodium ascorbate, and different concentrations of H2O2, on the physiochemical properties of myofibrillar protein (MP) from squid mantles, has been investigated. The effect of different exposure times to HRGS was also considered. Compared to non-oxidized MP, a significant (p < 0.05) increase in carbonyl content (more than 50% of its original content) and protein solubility, as well as in surface hydrophobicity, was observed in the oxidative MP. With different treatment times, a sharp decrease (p < 0.05) in sulfhydryl content was detected. In addition, hydroxyl radical treatment significantly reduced the MP gel's texture properties, whiteness and water holding capacity, especially at higher concentrations of H2O2. This observation could be attributed to extensive disorderly and less compact structure of MP gels. The results demonstrate the negative effect of HRGS on the structural and functional properties of MP from squid mantles.


Assuntos
Decapodiformes/metabolismo , Proteínas de Frutos do Mar/química , Animais , Ácido Ascórbico/química , Géis/química , Interações Hidrofóbicas e Hidrofílicas , Radical Hidroxila/química , Oxirredução , Reologia , Proteínas de Frutos do Mar/metabolismo , Solubilidade , Compostos de Sulfidrila/análise , Água/química
7.
Environ Sci Pollut Res Int ; 26(12): 11975-11987, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30825127

RESUMO

Cephalopods are important in the diets of humans and many other apex predators, and can play an important role in the bioaccumulation of metals. In this study, metal concentrations were analysed in the commercially and ecologically important southern arrow squid, Nototodarus sloanii (Gray 1849), from a heavily targeted fisheries area on New Zealand's Chatham Rise. A variety of tissue types were compared in order to assess the bioaccumulation in edible tissues (mantle and arms) and other organs (digestive gland, kidney, and hearts). Although metal concentrations varied among tissue types, the highest concentrations were found in the digestive gland (for Cd, Cu, Fe, Ni, Zn) and branchial hearts (for Cr, U). We report the first Cd concentrations for N. sloanii from the Chatham Rise, with mean values of 3.11 µg. g-1 in the mantle and 102.53 µg. g-1 in the digestive gland. Our data suggest that concentrations observed in the mantle tissue (which forms the majority of the muscle tissue) and digestive gland (the primary organ for metal accumulation) can be used to estimate the animal's total body burden for all metals analysed. The toxicological and dietary consequences for predators (including humans) feeding on arrow squid can be inferred from measurements of mass and metal concentration in these tissues. Arrow squid represent an important vector for Cd transfer within the pelagic Chatham Rise food web. These are the first recorded baseline data for metal concentrations for any squid in this oceanic region.


Assuntos
Decapodiformes/metabolismo , Monitoramento Ambiental , Metais/metabolismo , Poluentes Químicos da Água/metabolismo , Animais , Exposição Dietética/estatística & dados numéricos , Pesqueiros , Cadeia Alimentar , Contaminação de Alimentos/estatística & dados numéricos , Humanos , Metais/análise , Metais Pesados/análise , Nova Zelândia , Oceanos e Mares , Alimentos Marinhos/estatística & dados numéricos , Poluentes Químicos da Água/análise
8.
Environ Pollut ; 248: 159-165, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30784834

RESUMO

Plastic pollution is prevalent worldwide and affects marine wildlife from urbanized beaches to pristine oceanic islands. However, the ecological basis and mechanisms that result in marine animal ingestion of plastic debris are still relatively unknown, despite recent advances. We investigated the relationship between scavenging behavior and plastic ingestion using green turtles, Chelonia mydas, as a model. Diet analysis of C. mydas showed that sea turtles engaging in scavenging behavior ingested significantly more plastic debris than individuals that did not engage in this foraging strategy. We argue that opportunistic scavenging behavior, an adaptive behavior in most marine ecosystems, may now pose a threat to a variety of marine animals due to the current widespread plastic pollution found in oceans.


Assuntos
Decapodiformes/metabolismo , Ingestão de Alimentos , Comportamento Alimentar , Plásticos/metabolismo , Tartarugas/metabolismo , Poluentes da Água/metabolismo , Animais , Oceano Atlântico , Ecossistema , Cadeia Alimentar , Conteúdo Gastrointestinal/química , Resíduos
9.
Food Chem ; 283: 324-330, 2019 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-30722879

RESUMO

Relationship among pH, generation of free amino acids (FAAs), and Maillard browning was investigated in Japanese common squid during air drying at 40 °C to prevent its discoloration. In the surface color measurement, increase in the b* value which is an indicator for Maillard browning of the dried squid was mitigated at pH 4.0-6.0 and accelerated at pH 7.5-9.0. Adjusting the pH to 5.5 effectively suppressed increasing of the b* value (p < 0.05). Arginine (Arg) generation involved in the Maillard reaction, was inhibited in the dried squid at pH 5.5 (p < 0.05). Maillard reactivities between ribose and amino acids were lower at pH 4.0 than at pH 5.5 (p < 0.05). These results indicate that browning of the dried squid is significantly suppressed at pH 5.5. Moreover, suppression of Arg generation is more effective than weakening of Maillard reactivity between ribose and Arg, in mitigating Maillard browning of the dried squid at an acidic pH.


Assuntos
Aminoácidos/metabolismo , Decapodiformes/metabolismo , Alimentos Marinhos/análise , Aminoácidos/química , Animais , Arginina/metabolismo , Cor , Concentração de Íons de Hidrogênio , Reação de Maillard , Ribose/química , Ribose/metabolismo , Temperatura
10.
Mol Neurobiol ; 56(1): 56-60, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29675577

RESUMO

The synthesis of brain metabolic DNA (BMD) is modulated by learning and circadian oscillations and is not involved in cell division or DNA repair. Data from rats have highlighted its prevalent association with the mitochondrial fraction and its lack of identity with mtDNA. These features suggested that BMD could be localized in synaptosomes that are the major contaminants of brain mitochondrial fractions. The hypothesis has been examined by immunochemical analyses of the large synaptosomes of squid optic lobes that are readily prepared and identified. Optic lobe slices were incubated with 5-bromo-2-deoxyuridine (BrdU) and the isolated synaptosomal fraction was exposed to the green fluorescent anti-BrdU antibody. This procedure revealed that newly synthesized BrdU-labeled BMD is present in a significant percent of the large synaptosomes derived from the nerve terminals of retinal photoreceptor neurons and in synaptosomal bodies of smaller size. Synaptosomal BMD synthesis was strongly inhibited by actinomycin D. In addition, treatment of the synaptosomal fraction with Hoechst 33258, a blue fluorescent dye specific for dsDNA, indicated that native DNA was present in all synaptosomes. The possible role of synaptic BMD is briefly discussed.


Assuntos
DNA/metabolismo , Decapodiformes/metabolismo , Sinaptossomos/metabolismo , Animais , DNA/biossíntese , Dactinomicina/farmacologia , Sinaptossomos/efeitos dos fármacos
11.
J Exp Biol ; 221(Pt 19)2018 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-30111556

RESUMO

Squid are thought to obtain a large portion of their oxygen via simple diffusion across the skin in addition to uptake at the gills. Although this hypothesis has support from indirect evidence and is widely accepted, no empirical examinations have been conducted to assess the validity of this hypothesis. In this study, we examined cutaneous respiration in two squid species, Doryteuthis pealeii and Lolliguncula brevis, using a divided chamber to physically separate the mantle cavity and gills from the outer mantle surface. We measured oxygen consumption and ammonia excretion rates in the two compartments and found that, at rest, squid only obtain enough oxygen cutaneously to meet the demand of the skin tissue locally (12% of total) and excrete little ammonia across the skin. The majority of oxygen is obtained via the traditional branchial pathway. In light of these findings, we re-examine and discuss the indirect evidence that has supported the cutaneous respiration hypothesis.


Assuntos
Amônia/metabolismo , Decapodiformes/metabolismo , Consumo de Oxigênio/fisiologia , Animais , Especificidade da Espécie
12.
J Exp Biol ; 221(Pt 19)2018 10 10.
Artigo em Inglês | MEDLINE | ID: mdl-30111559

RESUMO

Ocean acidification is hypothesized to limit the performance of squid owing to their exceptional oxygen demand and pH sensitivity of blood-oxygen binding, which may reduce oxygen supply in acidified waters. The critical oxygen partial pressure (P crit), the P O2  below which oxygen supply cannot match basal demand, is a commonly reported index of hypoxia tolerance. Any CO2-induced reduction in oxygen supply should be apparent as an increase in P crit In this study, we assessed the effects of CO2 (46-143 Pa; 455-1410 µatm) on the metabolic rate and P crit of two squid species - Dosidicus gigas and Doryteuthis pealeii - through manipulative experiments. We also developed a model, with inputs for hemocyanin pH sensitivity, blood P CO2  and buffering capacity, that simulates blood oxygen supply under varying seawater CO2 partial pressures. We compare model outputs with measured P crit in squid. Using blood-O2 parameters from the literature for model inputs, we estimated that, in the absence of blood acid-base regulation, an increase in seawater P CO2  to 100 Pa (≈1000 µatm) would result in a maximum drop in arterial hemocyanin-O2 saturation by 1.6% at normoxia and a P crit increase of ≈0.5 kPa. Our live-animal experiments support this supposition, as CO2 had no effect on measured metabolic rate or P crit in either squid species.


Assuntos
Dióxido de Carbono/efeitos adversos , Decapodiformes/metabolismo , Oxigênio/sangue , Água do Mar/química , Equilíbrio Ácido-Base , Animais , Decapodiformes/efeitos dos fármacos , Modelos Biológicos , Especificidade da Espécie
13.
Food Chem ; 269: 212-219, 2018 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-30100426

RESUMO

To control discoloration of dried Japanese common squid (Todarodes pacificus) product, influence of 0-1% organic salts, including sodium gluconate (Na-gluconate), sodium citrate (Na-citrate), sodium phytate (Na-phytate), sodium benzoate (Na-benzoate) and sodium tartrate (Na-tartrate), on Maillard browning in the squid meat during air-drying was investigated. Changes in surface color of the dried squid were mitigated by the addition of organic salts. Organic salts also showed inhibitory effects on autolysis and generation of free amino acids (FAAs), especially arginine, in both the dried squid meat and the model solution. Moreover, Maillard reaction degree of the dried squid was decreased by using organic salts. The addition of 1% Na-citrate or 1% Na-phytate well suppressed the browning of the dried squid (p < 0.05). The results indicated that organic salts having strong chelating ability, including Na-citrate and Na-phytate, can prevent the browning of the dried squid product by inhibiting the generation of FAAs.


Assuntos
Decapodiformes/metabolismo , Conservação de Alimentos/métodos , Alimentos Marinhos/análise , Animais , Reação de Maillard , Carne , Sais
14.
J Mol Biol ; 430(21): 4102-4118, 2018 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-30120952

RESUMO

Photoreceptors of the squid Loligo pealei contain a G-protein-coupled receptor (GPCR) signaling system that activates phospholipase C in response to light. Analogous to the mammalian visual system, signaling of the photoactivated GPCR rhodopsin is terminated by binding of squid arrestin (sArr). sArr forms a light-dependent, high-affinity complex with squid rhodopsin, which does not require prior receptor phosphorylation for interaction. This is at odds with classical mammalian GPCR desensitization where an agonist-bound phosphorylated receptor is needed to break stabilizing constraints within arrestins, the so-called "three-element interaction" and "polar core" network, before a stable receptor-arrestin complex can be established. Biophysical and mass spectrometric analysis of the squid rhodopsin-arrestin complex indicates that in contrast to mammalian arrestins, the sArr C-tail is not involved in a stable three-element interaction. We determined the crystal structure of C-terminally truncated sArr that adopts a basal conformation common to arrestins and is stabilized by a series of weak but novel polar core interactions. Unlike mammalian arrestin-1, deletion of the sArr C-tail does not influence kinetic properties of complex formation of sArr with the receptor. Hydrogen-deuterium exchange studies revealed the footprint of the light-activated rhodopsin on sArr. Furthermore, double electron-electron resonance spectroscopy experiments provide evidence that receptor-bound sArr adopts a conformation different from the one known for arrestin-1 and molecular dynamics simulations reveal the residues that account for the weak three-element interaction. Insights gleaned from studying this system add to our general understanding of GPCR-arrestin interaction.


Assuntos
Arrestina/química , Arrestina/metabolismo , Decapodiformes/metabolismo , Domínios e Motivos de Interação entre Proteínas , Rodopsina/química , Rodopsina/metabolismo , Animais , Cinética , Modelos Moleculares , Simulação de Dinâmica Molecular , Fosforilação , Ligação Proteica , Conformação Proteica , Análise Espectral , Relação Estrutura-Atividade
15.
Artigo em Inglês | MEDLINE | ID: mdl-30165287

RESUMO

Marine phospholipids (MPLs) are valuable components that can be applied within diverse areas like nutrition, pharmacy, and medicine as well as basic scientific research, particularly due to their high concentration of long chain polyunsaturated fatty acids. In this work, lipidomics approach by UPLC-Q-Exactive Orbitrap/MS was used for the identification, quantification, comparison, and characterization of phospholipids (PLs) from three different marine resources (shrimp head, codfish roe, and squid gonad). In total, 310 PL molecular species containing 34 different structures of fatty acid chains were identified simultaneously by Lipidsearch software. Significant differences between three groups in the PL classes, compositions and contents were revealed. A list of characteristic PL species that represent significant differences among groups was determined by lipidomics analysis. Until now, the information about the deep comprehensive description of PL from marine sources is limited. Thus, this study will give a very potential starting point to develop MPL products and establish the quality standards for different marine raw materials.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Biologia Computacional/métodos , Gadiformes/metabolismo , Espectrometria de Massas/métodos , Penaeidae/metabolismo , Fosfolipídeos/análise , Animais , Decapodiformes/metabolismo , Ácidos Graxos/análise , Ácidos Graxos/química , Ácidos Graxos/metabolismo , Limite de Detecção , Modelos Lineares , Fosfolipídeos/química , Fosfolipídeos/metabolismo , Análise de Componente Principal , Reprodutibilidade dos Testes
16.
Bull Environ Contam Toxicol ; 101(1): 42-48, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29881941

RESUMO

We measured total mercury (Hg) and selenium (Se) concentrations as well as stable nitrogen (N) isotopic composition in flyingfish and squid muscle tissues from the eastern Indian Ocean and western South China Sea. The results showed that the mean Hg concentration in squid muscle from the western South China Sea was lower than that in the eastern Indian Ocean. The Hg concentrations in flyingfish and squid muscle samples were positively correlated with organism size (length and weight) and δ15N in all the study areas. Furthermore, we found a negative correlation between Se and Hg in molar content of flyingfish and squid muscle from the western South China Sea. The Se:Hg molar ratio was significantly negative correlated with fish weight and δ15N, suggesting that the Se:Hg molar ratio decreases with the increase of fish size and trophic level in the food web.


Assuntos
Beloniformes/metabolismo , Decapodiformes/metabolismo , Monitoramento Ambiental/métodos , Mercúrio/análise , Selênio/análise , Poluentes Químicos da Água/análise , Animais , China , Cadeia Alimentar , Oceano Índico , Mercúrio/metabolismo , Músculos/química , Alimentos Marinhos/análise , Selênio/metabolismo , Poluentes Químicos da Água/metabolismo
17.
Mater Sci Eng C Mater Biol Appl ; 89: 283-294, 2018 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-29752100

RESUMO

Collagen from marine organisms has a broad prospect in biomedical field, yet the knowledge on marine-derived type II collagen is rare. Herein, a novel type II collagen was successfully isolated from squid cartilage for the first time. After being characterized, the immunogenicity of squid type II collagen (SCII) was evaluated and compared with that of bovine type II collagen (BCII). Then investigations were further conducted for the impacts of SCII on pro-inflammatory macrophages and macrophage chemotaxis. The degenerative osteoarthritis (OA) -relieving effects of SCII were explored using OA rat model in vivo. Our results demonstrated that the isolated SCII maintained triple-superhelical structure of native collagen with high purity. Different from BCII, SCII presented no immunogenicity since it neither induced abnormal proliferation of lymphocytes in vitro nor changed the basic levels of IgM, IgG, anti-type II collagen IgG and CD4+/CD8+ lymphocytes ratio in vivo. Additionally, SCII also exerted prominent anti-inflammatory effects. SCII significantly reduced the production of pro-inflammatory cytokines by enhancing the activity of TCPTP and subsequently prompting the dephosphorylation of p-STAT1 in pro-inflammatory macrophages. Besides, it indirectly prevented hypertrophic changes of chondrocytes, and markedly impeded chemotaxis of macrophages. Moreover, inflammation condition in OA rats was significantly alleviated under treatment with SCII. These data suggested that the newly developed SCII could not only avoid the immunogenic risks of collagen derived from terrestrial animals, but more importantly, provide new choice for the control and treatment of OA.


Assuntos
Materiais Biocompatíveis/farmacologia , Colágeno Tipo II/farmacologia , Decapodiformes/metabolismo , Fator de Transcrição STAT1/metabolismo , Transdução de Sinais/efeitos dos fármacos , Animais , Anticorpos/sangue , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/uso terapêutico , Linfócitos T CD4-Positivos/citologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD8-Positivos/citologia , Linfócitos T CD8-Positivos/imunologia , Linfócitos T CD8-Positivos/metabolismo , Cartilagem/efeitos dos fármacos , Cartilagem/metabolismo , Cartilagem/patologia , Sobrevivência Celular/efeitos dos fármacos , Colágeno Tipo II/imunologia , Colágeno Tipo II/metabolismo , Modelos Animais de Doenças , Macrófagos/citologia , Macrófagos/imunologia , Macrófagos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Osteoartrite/tratamento farmacológico , Osteoartrite/patologia , Ratos , Ratos Sprague-Dawley , Membrana Sinovial/efeitos dos fármacos , Membrana Sinovial/metabolismo , Membrana Sinovial/patologia
18.
Biochim Biophys Acta Gene Regul Mech ; 1861(6): 586-593, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29729419

RESUMO

At night, Humboldt squid (Dosidicus gigas) rise to the ocean's surface to feed, but come morning, they descend into the ocean's oxygen minimum zone where they can avoid predators but must deal with severe hypoxia, high pressure, and very cold water. To survive this extreme environment, squid use various adaptations to enter a hypometabolic state characterized by metabolic rate suppression by 35-52%, relative to normoxic conditions. The molecular mechanisms facilitating this metabolic flexibility have yet to be elucidated in hypometabolic squid. Herein, we report the first investigation of the role of microRNAs, a rapid and reversible post-transcriptional master regulator of virtually all biological functions, in cephalopods. We examined expression levels of 39 highly-conserved invertebrate microRNAs in D. gigas brain, mantle muscle, and branchial heart, comparing hypoxic and normoxic conditions. Hypoxia-inducible microRNAs are potentially involved in facilitating neuroprotection, anti-apoptosis, and regenerative mechanisms in brain; inhibiting apoptosis and cell proliferation while conserving energy in heart; and limiting damage by reactive oxygen species and apoptosis in muscle. Rather than orchestrate global metabolic rate depression, the majority of hypoxia-inducible microRNAs identified are involved in promoting cytoprotective mechanisms, suggesting a regulatory role for microRNA in hypoxic marine invertebrates that sets the stage for mechanistic analyses.


Assuntos
Decapodiformes/metabolismo , Metabolismo Energético , Regulação da Expressão Gênica , Hipóxia/metabolismo , MicroRNAs/biossíntese , Animais , Apoptose , Decapodiformes/genética , Hipóxia/genética , MicroRNAs/genética , Especificidade de Órgãos/genética , Espécies Reativas de Oxigênio/metabolismo
19.
Molecules ; 23(4)2018 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-29570647

RESUMO

The peptide FMRFamide is one of the well-known peptides involved in multiple physiological processes in the phylum Mollusca. In this study, a FMRFamide gene (GenBank accession No. KJ933411) was identified in a cuttlefish species called Sepiella japonica and was designated as SjFMRFamide. The total length of the SjFMRFamide sequence was found to be 1880 bp while the open reading frame contained 996 bp encoding a protein of 331 amino acid residues with a predicted isoelectric point (pI) and molecular weight (MW) of 9.18 and 38.8 kDa along with a 333 bp 5'-untranslated region (UTR) and 551 bp 3'-UTR. The deduced SjFMRFamide precursor protein contains one signal peptide and expresses four kinds FMRFamide-related peptides including a single copy of FLRFamide, ALSGDAFLRFamide, and FIRFamide and multiple copies of FMRFamide. Results of phylogenetic relation analysis strongly indicated that the sequence of this gene shares high identity with the genes of known FMRFamides. Spatial expression analysis indicated the highest mRNA expression of SjFMRFamide in the brain of male and female cuttlefishes among the eight tissues analyzed. An in situ hybridization assay of the brain indicated that SjFMRFamide was transcribed in several functional lobes, which suggests that it might be related to many physiological regulatory mechanisms. This is the first study describing FMRFamide in S. japonica and the results may contribute to future studies of neuropeptide evolution or may prove useful for the development of aquaculture methods for this cuttlefish species.


Assuntos
Decapodiformes/metabolismo , FMRFamida/metabolismo , Peptídeos/metabolismo , Regiões 3' não Traduzidas/genética , Animais , Decapodiformes/genética , FMRFamida/genética , Moluscos/metabolismo , Neuropeptídeos/genética , Neuropeptídeos/metabolismo , Peptídeos/genética
20.
Mar Environ Res ; 133: 45-48, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29223596

RESUMO

Domoic acid (DA) is a neurotoxin that causes degenerative damage to brain cells and induces permanent short-term memory loss in mammals. In cephalopod mollusks, although DA is known to accumulate primarily in the digestive gland, there is no knowledge whether DA reaches their central nervous system. Here we report, for the first time, the presence of DA in brain tissue of the common octopus (Octopus vulgaris) and the European cuttlefish (Sepia officinalis), and its absence in the brains of several squid species (Loligo vulgaris, L. forbesi and Todarodes sagittatus). We argue that such species-specific differences are related to their different life strategies (benthic/nektobenthic vs pelagic) and feeding ecologies, as squids mainly feed on pelagic fish, which are less prone to accumulate phycotoxins. Additionally, the temporal persistence of DA in octopus' brain reinforces the notion that these invertebrates can selectively retain this phycotoxin. This study shows that two highly-developed invertebrate species, with a complex central nervous system, where glutamatergic transmission is involved in vertebrate-like long-term potentiation (LTP), have the ability of retaining and possibly tolerating chronic exposure to DA, a potent neurotoxin usually acting at AMPA/kainate-like receptors. Here, we filled a gap of information on whether cephalopods accumulated this neurotoxin in brain tissue, however, further studies are needed to determine if these organisms are neurally or behaviourally impaired by DA.


Assuntos
Encéfalo/metabolismo , Decapodiformes/metabolismo , Ácido Caínico/análogos & derivados , Toxinas Marinhas/metabolismo , Octopodiformes/metabolismo , Animais , Monitoramento Ambiental , Ácido Caínico/metabolismo , Intoxicação por Frutos do Mar
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